| [diagnosis and therapeutic control of gonorrhea. effects and costs]. | | 2011 | 3787810 |
| brain death sans frontières. | | 2011 | 3127708 |
| melanoma antigen expression and metastatic ability of mutant b16 melanoma clones. | the biological functions of murine melanoma-associated antigens recognized by monoclonal antibodies (mabs) (m562, m622 and m2590) were examined by using mutant clones which differed in their degree of expression of these antigens. four clones of high expressors of 3 types of antigen (mea group), 5 clones of low or non-expressors of m562- and m622-recognizing antigens (meb group) and 4 clones of non-expressor of gm3 recognized by m2590 (mec group) were used. attachment of these clones to componen ... | 2011 | 3053466 |
| dermal, subcutaneous, and tendon xanthomas: diagnostic markers for specific lipoprotein disorders. | many patients with lipoprotein disorders are at increased risk for the development of premature atherosclerosis and, less commonly, other disorders that cause systemic morbidity. in some of these patients, xanthomas also develop and provide cutaneous markers for the lipoprotein disorder. as advances in molecular biology refine our understanding of lipoprotein metabolism, it has become increasingly clear that several types of xanthomas are associated with specific disease states. this article pre ... | 2011 | 3042820 |
| [multilevel structure isolation using modules based on the example of thoracic radiography]. | conventional chest radiographs were digitized and processed by multilevel band pass filtering. by this procedure different structural components are isolated according to their local spatial frequencies. in chest radiographs a textural component can be separated from a skeletal component. evaluation of radiological images is facilitated because changes can be looked for at relevant levels. a reconstruction of the image is possible, using different weighting factors to enhance interesting structu ... | 2011 | 3025963 |
| [dental aspects of bidloo's "anatomy" (1685), illustrated by lairesse and plagerized by cowper (iii)]. | | 2012 | 3531781 |
| inflammatory bowel disease: one condition or two? | demographic data obtained from epidemiological studies of crohn's disease and ulcerative proctocolitis in regio leiden (leiden health care region) were compared. ulcerative colitis (6.8 cases/10(5) population/year) had an incidence almost twice that of crohn's disease (3.9/10(5)/year; p less than 0.001). each disease occurred with equal frequency in both sexes and diagnosis of the diseases occurred at similar ages (spearman's correlation coefficient = 0.84; p less than 0.01). where ulcerative co ... | 2012 | 3443227 |
| women in a changing profession. | | 2012 | 3434574 |
| [the use of the large saphenous vein in-situ in the treatment of ischemia of the leg and foot]. | | 2012 | 3406073 |
| sinus arrest associated with clonidine therapy. | a 65-year-old male with diabetes, hypertension, and mild renal failure developed dizziness and syncope one week after starting clonidine 0.45 mg/day. a continuous ecg recording revealed sinus bradycardia, nodal rhythm, and multiple episodes of sinus arrest lasting up to 4.5 seconds. upon discontinuation of clonidine, serial continuous ecg recordings revealed gradual decrease in the number and duration of the sinus arrest episodes, until their complete disappearance shortly after the third day of ... | 2012 | 3349661 |
| effects of increased cell mass and altered gene dosage on the timing of initiation of macronuclear dna synthesis in paramecium tetraurelia. implications for cell cycle regulation. | in paramecium, cell mass and macronuclear dna content can vary substantially, and both variables affect the timing of initiation of macronuclear dna synthesis. cells normally begin macronuclear dna synthesis at 0.25 in the cell cycle when the mean cell mass is about 119% of the initial value. gene mutations were used to alter cell size by temporarily blocking cell cycle progression and to change dna content by altering the segregation pattern of macronuclear material to daughter nuclei at fissio ... | 2013 | 3709689 |
| the role of the clinical environment in the translation of research into practice. | | 2013 | 3636224 |
| [congress 86. there is nothing too small to be discussed]. | | 2013 | 3638012 |
| hypertrophy of heart with outflow tract obstruction and myocardial necrosis in an infant with multiple congenital malformations. | | 2014 | 4252909 |
| identification of the bicarbonate secretory cell of the turtle bladder. | the turtle bladder consists primarily of two mucosal cell types, mitochondrial rich (mr) and granular (g) cells. the mr cells secrete h+. in addition, the bladder secretes hco3-, an energy-requiring cl(-)-dependent transport system. these studies were designed to identify the cell type responsible for hco3- secretion. specific hco3- and h+ secretory inhibitors were added to alter o2 consumption of mr and g cells. cells were separated by ficoll density centrifugation. ouabain (10(-4) m) was used ... | 2014 | 4073269 |
| toxicity in hela cells mediated by plastic wraps. | | 2015 | 4690021 |
| [anti-arrhythmia agents. prehospital therapy and prevention of arrhythmia in acute myocardial infarct. what advice should the general practitioner get?]. | | 2015 | 4691029 |
| [necrosing scleritis]. | | 2015 | 4690012 |
| [clinical results with amikacin (bb-k8) in surgical field (author's transl)]. | | 2015 | 4546598 |
| [smallpox]. | | 2015 | 4483649 |
| [guarding the health of the soviet people]. | | 2015 | 4495192 |
| [effect of 3,4-dihydroxyphenylalanine (levodopa) in hepatic coma]. | | 2016 | 5037838 |
| [clinico-genetic considerations on basilar impression. ii. the role of the hereditary factor in basilar impression]. | | 2016 | 4978197 |
| oral lesions. | | 2016 | 4931041 |
| [ideas of lenin and bulgarian medicine]. | | 2016 | 4921010 |
| dental changes in burkitt's lymphoma. | | 2016 | 4718558 |
| innervation of the hand: an electromyographic study. | | 2016 | 4732560 |
| newer diagnostic methods. | | 2015 | 4453578 |
| dual-ended, multifunction tools for microsurgery. | a new concept in microsurgical instrumentation is herein described and the rationale for its design presented in the context of surgical efficiency. this new concept employs bipolarity or dual-endedness as a means to enable the microsurgeon to have access to various instruments without the need to exchange tools. the elimination of instrument exchange has increased the speed with which a microsurgeon can perform a variety of procedures as well as decrease fatigue associated with continuous visua ... | 2006 | 4088033 |
| successful pregnancy in primary glomerular disease. | the course of 66 pregnancies was studied in 48 women with primary glomerular diseases. in all cases diagnoses were established by biopsy before pregnancy. they were: membranoproliferative glomerulonephritis in 16 patients, focal glomeruloesclerosis in 13, iga nephropathy in 10, membranous nephropathy in seven and focal glomerulonephritis in two women. the clinical status of the nephropathy before conception was that 43 had only mild renal dysfunction, five had moderate renal insufficiency, serum ... | 2013 | 3820941 |
| [treatment of diseases of permanent teeth with unformed roots. 2. treatment of diseases of the pulp]. | | 2015 | 4508803 |
| [la fontaine and the apothecaries]. | | 2016 | 4929537 |
| editorial: the heavy chain diseases. | | 2016 | 4844731 |
| is hypertension treated adequately? | in hypertensive patients, morbidity and mortality from the malignant phase, stroke, left ventricular failure, coronary disease and progressive renal failure are closely linked to the level of blood pressure. this relationship between blood pressure and risk can be demonstrated also when blood pressure is lowered with antihypertensive agents. thus, it appears that the treated blood pressure level is a much stronger indicator of prognosis than is the initial untreated value. however, in the last 2 ... | 2012 | 3443056 |
| the t cell receptor: its repertoire and role in thymocyte development. | | 2012 | 3498291 |
| fifty years of penicillin. | | 1973 | 380755 |
| quality of care on a psychogeriatric nursing unit in finland: focus on interaction skills. | | 1979 | 1319942 |
| [orthodontic and surgical approach to mandibular prognathia. prevention of recurrences]. | the authors emphasize the necessity for a combined orthodontic and surgical approach to the problems posed by the treatment of mandibular prognathia. they feel that the major source of recurrences is related to the alveolar changes which accompany the new basal maxillo-mandibular relationship and the resultant modifications in muscular equilibrium. the prevention of recurrences must therefore take into account the prediction of adaptive remodelling of the alveolar processes. | 1983 | 272713 |
| neonatal shock in the first postnatal day. | advances in neonatal medicine have markedly reduced mortality in preterm infants. current emphasis should be placed on early detection and treatment of neonatal hypotension and shock. ideal clinical management includes perinatal recognition of low birth weight infants at risk for hypotension. all critically ill preterm infants should be closely monitored for tachycardia, hypotension, and/or decreased urinary output. by use of noninvasive blood pressure screening techniques, hypotensive infants m ... | 1983 | 347923 |
| [recent research on amalgams]. | | 1983 | 272596 |
| new relief for menstrual discomfort. | | 1991 | 257720 |
| [a comparative study of acid mucopolysaccharides of paranasal sinus mucous membrane in normal and chronic sinusitis (author's transl)]. | | 2003 | 162040 |
| [the significance of the determination of catecholamines and their metabolites in neuroblastomas]. | a method for the determination of catecholamines and their metabolites in the urine was established. the specimens were previously cleaned by ion exchangers and the components were separated by a 'reversed phase' ionic pair chromatography, determined by an electrochemical 'wall-jet'-detector. this method was applied in order to find a diagnostic marker in case of neuroblastoma diseases. in 85 per cent of the patients (n = 13) we stated an increased ejection of dopamine. | 2006 | 2095056 |
| iron status markers in hereditary haemochromatosis: distinction between individuals being homozygous and heterozygous for the haemochromatosis allele. | iron status markers, serum iron, serum transferrin, transferrin saturation and serum ferritin were analysed in 162 homozygous patients with clinical haemochromatosis, in 12 homozygous relatives with preclinical haemochromatosis, in 84 heterozygous, and in 9 normal subjects. in the distinction between homozygous patients with clinical haemochromatosis and heterozygotes, transferrin saturation and serum ferritin showed the highest diagnostic efficiencies. a diagnostic efficiency of 0.97 was obtain ... | 2007 | 1954989 |
| [socioeconomic aspects of depression]. | | 2009 | 2629567 |
| the history of the use of vacuum extraction. | | 2010 | 3047531 |
| impact of pharmacist intervention on oral theophylline therapy in adult inpatients. | the impact of clinical pharmacy services on the utilization of oral theophylline therapy was evaluated in a ten-week study involving 138 adult inpatients. the study initially involved an independent prospective five-week audit of theophylline use, in which clinical pharmacists monitored theophylline therapy and any interventions were designed so as not to influence future actions taken by medical officers with regard to oral theophylline therapy. the second part of the study involved active inte ... | 2011 | 3428141 |
| metasternal gland secretion of the locust tree borer, megacyllene robiniae (coleoptera: cerambycidae). | 1. the volatile components of metasternal gland extracts of male and female megacyllene robiniae have been analyzed by gas chromatography-mass spectroscopy. 2. the major component was identified as 2-(1,3-hexadien-1-yl)-5-methyltetrahydrofuran, a new natural product. 3. 1-phenylethanol is present only in male extracts. 4. acetates of hexadecanol and octadecanol are also present. | 2011 | 3224513 |
| [basic trends and tasks of applied anatomical research today]. | | 2012 | 3435260 |
| sociological and medical aspects of nutrition. | | 2012 | 3376489 |
| hypothermia and the action of neuromuscular blocking agents. | | 2012 | 3354819 |
| depression and immunity. lymphocyte function in ambulatory depressed patients, hospitalized schizophrenic patients, and patients hospitalized for herniorrhaphy. | mitogen-induced lymphocyte stimulation responses in ambulatory patients with major depressive disorder did not differ from those of matched controls. lymphocyte responses in hospitalized schizophrenic patients and in patients hospitalized for elective herniorrhaphy similarly did not differ from responses of controls. the number of peripheral-blood t cells was decreased among the ambulatory depressed patients but not in the schizophrenic patients. these findings, together with previously reported ... | 2012 | 3872111 |
| the official opening of the s.a. nurses memorial centre. | | 2013 | 4498805 |
| the official opening of the s.a. nurses memorial centre. | | 2013 | 4498805 |
| "what is," and "what should be?". | | 2013 | 3870712 |
| [medical and technologic aspects of the manifestation of silicosis in workers in the prvomajska foundry, zagreb]. | | 2013 | 3842066 |
| [research--the source of medical progress]. | | 2013 | 3842698 |
| [the erythrocyte as a potential container for directed transport of drugs to damaged parts of the circulatory bed]. | | 2013 | 3707723 |
| theory on thermal probe arrays for the distinction between the convective and the perfusive modalities of heat transfer in living tissues. | recent suggestions for an improved model of heat transfer in living tissues emphasize the existence of a convective mode due to flowing blood in addition to, or even instead of, the perfusive mode, as proposed in pennes' "classic" bioheat equation. in view of these suggestions, it might be beneficial to develop a technique that will enable one to distinguish between these two modes of bioheat transfer. to this end, a concept that utilizes a multiprobe array of thermistors in conjunction with a r ... | 2013 | 3695437 |
| [medical care problems of patients with delayed consolidation]. | | 2013 | 3587953 |
| [choice of a calcium antagonist in chronic stable angina]. | | 2013 | 4093052 |
| [thyrotoxic crisis]. | | 2014 | 4193806 |
| an unusual cause of neck swelling in an elderly female. | | 2014 | 4083241 |
| [the 58th annual meeting of the japanese orthopedic association. gifu, april 7-9, 1985. abstracts]. | | 2014 | 4078426 |
| introduction to panel: perspectives on the nature of psychic reality. | | 2014 | 4056297 |
| effects of hydralazine on pressure-volume and stress-volume relations in congestive heart failure secondary to idiopathic dilated cardiomyopathy. | the mechanism by which hydralazine improves cardiac function in patients with heart failure is not well characterized. hydralazine may improve left ventricular (lv) function by decreasing afterloading wall stress or by increasing myocardial contractility. the effect of intravenous hydralazine was assessed in 8 patients with severe idiopathic dilated cardiomyopathy. hydralazine increased stroke volume index (from 24 +/- 8 to 40 +/- 9 ml/m2, p less than 0.01) and decreased systemic vascular resist ... | 2014 | 4050708 |
| prison-psychiatry: medical or correctional discipline? | | 2014 | 4010493 |
| [a case of primary adenocarcinoma of the cervix of probable origin from an area of endometriosis]. | a case of primary adenocarcinoma of the cervix of probable endometrial origin is described in a 37 year-old woman. | 2014 | 3974935 |
| low doses of progestogen as an approach to fertility control. | | 2014 | 4158852 |
| what is visual training: a patient education handout. | | 2014 | 3968385 |
| receptive-field symmetry probed using converging gratings. | contrast-sensitivity functions (csf's) for converging and diverging gratings were obtained under voluntary fixation at several field widths and under retinal stabilization at one field width. in general, these types of gratings had similar csf's at all temporal frequencies tested, a result that can be explained by plausible spatiotemporal receptive-field models possessing spatial antisymmetry but not by models possessing pure (even) spatial symmetry. phase condition at the central line in conver ... | 2014 | 3973760 |
| [mechanisms of adaptation to physical exertion in patients with an expiratory obstructive syndrome]. | | 2014 | 4096445 |
| toxicokinetics of lithium intoxication treated by hemodialysis. | a 66-year old female on long-term lithium therapy, developed neurologic toxicity with coma and convulsions. the treatment consisted artificial ventilation, fluid and electrolyte infusions and a six hour hemodialysis (hd) on days 2, 3 and 4. the convulsions disappeared on day 4; the patient regained consciousness on day l2 and recovered with slight sequelae. toxicokinetic studies were conducted for a period of 12 days. the lithium (li) serum concentration fell from 4.4 mmol/l on admission to 2.16 ... | 2014 | 3938485 |
| measurement of color thresholds. | it is generally believed that some degree of defective color vision is frequently acquired along with certain ocular and systemic disorders. precise definition of the nature and extent of the color defects has not been possible because of the limitations inherent in the tests currently available for clinical use. we undertook to define the defects in terms of thresholds of discrimination to each color, plotted on a color circle similar to munsell's uniform chromaticity scale diagram. furthermore ... | 2014 | 3942184 |
| agglutination of an edta blood sample caused by an edta-dependent panagglutinin. | a novel example of an edta-dependent panagglutinin is described. a blood sample drawn into edta for a routine hematologic workup demonstrated strong agglutination due to edta-dependent panagglutinins. previous examples have been detected because of discrepant abo results. this is the first report of an edta-dependent panagglutinin that caused agglutination in the edta sample collection tube and a false positive direct antiglobulin test. | 2014 | 3920896 |
| [sequential angio-urography (sau) with photographic image subtraction]. | urography was performed in 250 patients: the technique of bolus injection of a large quantity of contrast medium was used in every case. by means of rapid seriography the early vascular phase was shown and reproduced, when necessary, with image subtraction. the abdominal aorta and the renal arteries were demonstrated in 97.6% of cases. altogether, in 34.4% of cases the existence of pathological conditions non demonstrable by conventional urography was ascertained; in 12.4% of cases extrarenal pa ... | 2014 | 3911305 |
| [medical education in the 18th century. on the 325th anniversary of the birth of friedrich hoffmann (1660-1742)]. | the 325th anniversary of friedrich hoffmann's birthday gave rise to remind of the early high period of the faculty of medicine of halle university. several significant scientific achievements are connected with hoffmann's name, but at the same time also the organisation of studies and examinations in halle. the educational practices of his time were demonstrated with the help of preserved documentations. | 2014 | 3904240 |
| [simulation studies on the behavior of various automatic diagnostic procedures]. | | 2015 | 4617105 |
| [china 1971]. | | 2015 | 4481286 |
| behavioral studies of noise-induced hearing loss in primates: loudness recruitment. | | 2015 | 4351038 |
| comment on the method of determining the trapped volume of plasma after centrifugation, based on the correlation between the trapped volume and the electrical conductivity. | | 2016 | 4964894 |
| the particulate graft in tumor surgery. | | 2016 | 4942608 |
| [racemization during peptide synthesis]. | | 2016 | 4881545 |
| [bibliographic tudy of nursing. present publications in nursing. 2]. | | 2016 | 4877474 |
| toxoplasma testing. | | 2016 | 4739210 |
| type iii secretion system translocon component eseb forms filaments on and mediates autoaggregation of and biofilm formation by edwardsiella tarda. | the type iii secretion system (t3ss) of edwardsiella tarda plays an important role in infection by translocating effector proteins into host cells. eseb, a component required for effector translocation, is reported to mediate autoaggregation of e. tarda. in this study, we demonstrate that eseb forms filamentous appendages on the surface of e. tarda and is required for biofilm formation by e. tarda in dulbecco's modified eagle's medium (dmem). biofilm formation by e. tarda in dmem does not requir ... | 2015 | 26116669 |
| rna-seq liver transcriptome analysis reveals an activated mhc-i pathway and an inhibited mhc-ii pathway at the early stage of vaccine immunization in zebrafish. | zebrafish (danio rerio) is a prominent vertebrate model of human development and pathogenic disease and has recently been utilized to study teleost immune responses to infectious agents threatening the aquaculture industry. in this work, to clarify the host immune mechanisms underlying the protective effects of a putative vaccine and improve its immunogenicity in the future efforts, high-throughput rna sequencing technology was used to investigate the immunization-related gene expression pattern ... | 2012 | 22805612 |
| edwardsiella tarda mlic, a lysozyme inhibitor that participates in pathogenesis in a manner that parallels ivy. | edwardsiella tarda, a bacterial pathogen to farmed fish as well as humans, possesses the genes of two lysozyme inhibitors, ivy and mlic (ivy(et) and mlic(et)). we recently studied ivyet and found it to be implicated in e. tarda virulence. in the present study, we characterized mlic(et) in comparison with ivy(et) in a turbot model. mlic(et) contains the fwskg motif and two cysteines (c33 and c98) that are highly conserved in subgroup 1 mlics but are of unknown functional importance. to examine th ... | 2014 | 25404031 |
| edwardsiella tarda hfq: impact on host infection and global protein expression. | hfq is an rna-binding protein that plays an important role in many cellular processes. in this study, we examined the biological effect of the hfq of edwardsiella tarda, a severe fish pathogen with a broad host range that includes humans. to facilitate the study, a markerless hfq in-frame deletion wild type, txhfq, was constructed. compared to the wild type tx01, txhfq exhibited (i) retarded planktonic and biofilm growth, (ii) decreased resistance against oxidative stress, (iii) attenuated overa ... | 2014 | 24568370 |
| edwardsiella tarda esce (orf13 protein) is a type iii secretion system-secreted protein that is required for the injection of effectors, secretion of translocators, and pathogenesis in fish. | the type iii secretion system (t3ss) of edwardsiella tarda is crucial for its intracellular survival and pathogenesis in fish. the orf13 gene (esce) of e. tarda is located 84 nucleotides (nt) upstream of esrc in the t3ss gene cluster. we found that esce is secreted and translocated in a t3ss-dependent manner and that amino acids 2 to 15 in the n terminus were required for a completely functional t3ss in e. tarda. deletion of esce abolished the secretion of t3ss translocators, as well as the secr ... | 2015 | 26459509 |
| edwardsiella tarda ivy, a lysozyme inhibitor that blocks the lytic effect of lysozyme and facilitates host infection in a manner that is dependent on the conserved cysteine residue. | edwardsiella tarda is a gram-negative bacterial pathogen with a broad host range that includes fish and humans. in this study, we examined the activity and function of the lysozyme inhibitor ivy (named ivyet) identified in the pathogenic e. tarda strain tx01. ivyet possesses the ivy signature motif ckphdc in the form of (82)cqphnc(87) and contains several highly conserved residues, including a tryptophan (w55). for the purpose of virulence analysis, an isogenic tx01 mutant, txivy, was created. t ... | 2013 | 23817616 |
| intramacrophage infection reinforces the virulence of edwardsiella tarda. | edwardsiella tarda is an important pathogenic bacterium that can replicate in macrophages. however, how the intramacrophage infection process affects the virulence of this bacterium is essentially unknown. here, we show that e. tarda replicates and induces a caspase-1-dependent cell pyroptosis in a murine macrophage model. via pyroptosis, intracellular e. tarda escapes to the extracellular milieu, forming a unique bacterial population. being different from the bacteria cultured alone, this uniqu ... | 2016 | 26953340 |
| edwardsiella tarda-induced inhibition of apoptosis: a strategy for intracellular survival. | edwardsiella tarda is a gram-negative bacterial pathogen that can infect a wide range of freshwater and marine fish. one salient feature of e. tarda is the ability to survive and replicate in various host cells. in this study, we observed that e. tarda replicated robustly in the zebrafish cell line zf4, and that e. tarda-infected cells exhibited no detectable signs of apoptosis. global transcriptome analysis and quantitative real-time rt-pcr revealed that e. tarda infection generally significant ... | 2016 | 27471679 |
| sialic acid utilization by cronobacter sakazakii. | the cronobacter genus is composed of seven species, and can cause infections in all age groups. of particular concern is c. sakazakii, as this species is strongly associated with severe and often fatal cases of necrotizing enterocolitis and meningitis in neonates and infants. whole genome sequencing has revealed that the nanakt gene cluster required for the utilisation of exogenous sialic acid is unique to the c. sakazakii species (esa_03609-13).sialic acid is found in breast milk, infant formul ... | 2013 | 23706082 |
| identification and functional characterization of the novel edwardsiella tarda effector esej. | edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and gastro- and extraintestinal infections in humans. the type iii secretion system (t3ss) of e. tarda has been identified as a key virulence factor that contributes to pathogenesis in fish. however, little is known about the associated effectors translocated by this t3ss. in this study, by comparing the profile of secreted proteins of the wild-type ppd130/91 and its t3ss atpase δesan mutant, we ide ... | 2015 | 25667268 |
| near-drowning-associated pneumonia with bacteremia caused by coinfection with methicillin-susceptible staphylococcus aureus and edwardsiella tarda in a healthy white man: a case report. | edwardsiella tarda is an enterobacteriaceae found in aquatic environments. extraintestinal infections caused by edwardsiella tarda in humans are rare and occur in the presence of some risk factors. as far as we know, this is the first case of near-drowning-associated pneumonia with bacteremia caused by coinfection with methicillin-susceptible staphylococcus aureus and edwardsiella tarda in a healthy patient. | 2016 | 27423181 |
| antimicrobial and herbal drug resistance in enteric bacteria isolated from faecal droppings of common house lizard/gecko (hemidactylus frenatus). | from 194 faecal dropping samples of common house geckos collected from offices (60), houses (88), integrated farm units (ifs,18) and hostels, guest houses, and dining rooms of different canteen/mess (hgm, 28), 326 bacterial isolates of enteric bacteria belonging to 17 genera and 34 species were detected. escherichia coli were the most frequently (39) isolated followed by citrobacter freundii (33), klebsiella pneumonia (27), salmonella indica (12), enterobacter gergoviae (12), and ent. agglomeran ... | 2013 | 24223595 |
| simultaneous detection of multiple fish pathogens using a naked-eye readable dna microarray. | we coupled 16s rdna pcr and dna hybridization technology to construct a microarray for simultaneous detection and discrimination of eight fish pathogens (aeromonas hydrophila, edwardsiella tarda, flavobacterium columnare, lactococcus garvieae, photobacterium damselae, pseudomonas anguilliseptica, streptococcus iniae and vibrio anguillarum) commonly encountered in aquaculture. the array comprised short oligonucleotide probes (30 mer) complementary to the polymorphic regions of 16s rrna genes for ... | 2012 | 22736973 |
| enterobacteria identification and detection of diarrheagenic escherichia coli in a port complex. | the port complex of maranhão (pcm) is the second largest port complex in brazil, receiving ships with large volumes of ballast water. to evaluate the microbiological quality of its waters, physicochemical parameters (ph and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic escherichia coli strains were analyzed. in order to identify the presence of e. coli virulence genes target regions of the stx, elt, est, aggr, cvd432, ipah ... | 2014 | 25477930 |
| comparative genomics reveals that a fish pathogenic bacterium edwardsiella tarda has acquired the locus of enterocyte effacement (lee) through horizontal gene transfer. | edwardsiella tarda is an enterobacterium which causes edwardsiellosis, a fatal disease of cultured fishes such as red sea bream, eel, and flounder. preventing the occurrence of e. tarda infection has thus been an important issue in aquaculture. e. tarda has been isolated from other animals and from many environments; however, the relationship between the genotype and evolutionary process of this pathogen is not fully understood. to clarify this relationship, we sequenced and compared the genomes ... | 2013 | 24053667 |