| the complete campylobacter jejuni transcriptome during colonization of a natural host determined by rnaseq. | campylobacter jejuni is a major human pathogen and a leading cause of bacterial derived gastroenteritis worldwide. c. jejuni regulates gene expression under various environmental conditions and stresses, indicative of its ability to survive in diverse niches. despite this ability to highly regulate gene transcription, c. jejuni encodes few transcription factors and its genome lacks many canonical transcriptional regulators. high throughput deep sequencing of mrna transcripts (termed rnaseq) has ... | 2013 | 23991199 |
| campylobacter jejuni pdxa affects flagellum-mediated motility to alter host colonization. | vitamin b6 (pyridoxal-5'-phosphate, plp) is linked to a variety of biological functions in prokaryotes. here, we report that the pdxa (putative 4-hydroxy-l-threonine phosphate dehydrogenase) gene plays a pivotal role in the plp-dependent regulation of flagellar motility, thereby altering host colonization in a leading foodborne pathogen, campylobacter jejuni. a c. jejuni pdxa mutant failed to produce plp and exhibited a coincident loss of flagellar motility. mass spectrometric analyses showed a ... | 2013 | 23936426 |
| divergent mechanisms of interaction of helicobacter pylori and campylobacter jejuni with mucus and mucins. | helicobacter pylori and campylobacter jejuni colonize the stomach and intestinal mucus, respectively. using a combination of mucus-secreting cells, purified mucins, and a novel mucin microarray platform, we examined the interactions of these two organisms with mucus and mucins. h. pylori and c. jejuni bound to distinctly different mucins. c. jejuni displayed a striking tropism for chicken gastrointestinal mucins compared to mucins from other animals and preferentially bound mucins from specific ... | 2013 | 23716616 |
| antimicrobial resistance mechanisms among campylobacter. | campylobacter jejuni and campylobacter coli are recognized as the most common causative agents of bacterial gastroenteritis in the world. humans most often become infected by ingesting contaminated food, especially undercooked chicken, but also other sources of bacteria have been described. campylobacteriosis is normally a self-limiting disease. antimicrobial treatment is needed only in patients with more severe disease and in those who are immunologically compromised. the most common antimicrob ... | 2013 | 23865047 |
| adaptive mechanisms of campylobacter jejuni to erythromycin treatment. | macrolide is the drug of choice to treat human campylobacteriosis, but campylobacter resistance to this antibiotic is rising. the mechanisms employed by campylobacter jejuni to adapt to erythromycin treatment remain unknown and are examined in this study. the transcriptomic response of c. jejuni nctc 11168 to erythromycin (ery) treatment was determined by competitive microarray hybridizations. representative genes identified to be differentially expressed were further characterized by constructi ... | 2013 | 23767761 |
| comparison of genotypes and antibiotic resistances of campylobacter jejuni and campylobacter coli on chicken retail meat and at slaughter. | multilocus sequence typing (mlst) and antibiotic resistance patterns of campylobacter jejuni and campylobacter coli from retail chicken meat showed high overlap with isolates collected at slaughterhouses, indicating little selection along the production chain. they also showed significant common sequence types with human clinical isolates, revealing chicken meat as a likely source for human infection. | 2013 | 23584778 |
| campylobacteriosis outbreak associated with consumption of undercooked chicken liver pâté in the east of england, september 2011: identification of a dose-response risk. | a foodborne outbreak with 49 cases (22 culture positive for campylobacter sp.) following a wedding party in the east of england was investigated. a retrospective cohort study identified an association between consumption of chicken liver pâté and infection with campylobacter jejuni/coli. there was a statistically significant association between dose (amount of chicken liver pâté eaten) and the risk of disease ['tasted': odds ratio (or) 1·5, 95% confidence interval (ci) 0·04-∞; 'partly eaten': or ... | 2013 | 23711104 |
| genome-wide association analysis of avian resistance to campylobacter jejuni colonization identifies risk locus spanning the cdh13 gene. | the enteropathogen campylobacter jejuni is a major worldwide health and economic burden, being one of the leading causes of bacterial gastroenteritis and commonly linked to postinfectious onset of autoimmune disease. chickens are a major vector for human infection and even though variation in avian colonization level is heritable, no previous studies have identified regions of the genome associated with colonization resistance. we performed a genome-wide association study of resistance to c. jej ... | 2013 | 23550144 |
| n-glycosylation of campylobacter jejuni surface proteins promotes bacterial fitness. | campylobacter jejuni is the etiologic agent of human bacterial gastroenteritis worldwide. in contrast, despite heavy colonization, c. jejuni maintains a commensal mode of existence in chickens. the consumption of contaminated chicken products is thought to be the principal mode of c. jejuni transmission to the human population. c. jejuni harbors a system for n-linked protein glycosylation that has been well characterized and modifies more than 60 periplasmic and membrane-bound proteins. however, ... | 2013 | 23460522 |
| effects of lipooligosaccharide inner core truncation on bile resistance and chick colonization by campylobacter jejuni. | campylobacter jejuni is the most common bacterium that causes diarrhea worldwide, and chickens are considered the main reservoir of this pathogen. this study investigated the effects of serial truncation of lipooligosaccharide (los), a major component of the outer membrane of c. jejuni, on its bile resistance and intestinal colonization ability in chickens. genes encoding manno-heptose synthetases or glycosyltransferases were inactivated to generate isogenic mutants. serial truncation of the los ... | 2013 | 23437265 |
| practicalities of using non-local or non-recent multilocus sequence typing data for source attribution in space and time of human campylobacteriosis. | in this study, 1208 campylobacter jejuni and c. coli isolates from humans and 400 isolates from chicken, collected in two separate periods over 12 years in the netherlands, were typed using multilocus sequence typing (mlst). statistical evidence was found for a shift of st frequencies in human isolates over time. the human mlst data were also compared to published data from other countries to determine geographical variation. because only mlst typed data from chicken, taken from the same time po ... | 2013 | 23405107 |
| a "successful allele" at campylobacter jejuni contingency locus cj0170 regulates motility; "successful alleles" at locus cj0045 are strongly associated with mouse colonization. | campylobacter jejuni is an important foodborne pathogen of humans and its primary reservoir is the gastrointestinal (gi) tract of chickens. our previous studies demonstrated that phase variation to specific "successful alleles" at c. jejuni contingency loci cj0045 (successful alleles carry 9g or 10g homopolymeric tracts) and cj0170 (successful allele carries a 10g homopolymeric tract) in c. jejuni populations is strongly associated with colonization and enteritis in c57bl/6 il-10 deficient mice. ... | 2013 | 23541212 |
| reduction of campylobacter jejuni in broiler chicken by successive application of group ii and group iii phages. | bacteriophage treatment is a promising tool to reduce campylobacter in chickens. several studies have been published where group ii or group iii phages were successfully applied. however, these two groups of phages are different regarding their host ranges and host cell receptors. therefore, a concerted activity of group ii and group iii phages might enhance the efficacy of a treatment and decrease the number of resistant bacteria. | 2014 | 25490713 |
| reducing campylobacter jejuni colonization of poultry via vaccination. | campylobacter jejuni is a leading bacterial cause of human gastrointestinal disease worldwide. while c. jejuni is a commensal organism in chickens, case-studies have demonstrated a link between infection with c. jejuni and the consumption of foods that have been cross-contaminated with raw or undercooked poultry. we hypothesized that vaccination of chickens with c. jejuni surface-exposed colonization proteins (secps) would reduce the ability of c. jejuni to colonize chickens, thereby reducing th ... | 2014 | 25474206 |
| chicken juice enhances surface attachment and biofilm formation of campylobacter jejuni. | the bacterial pathogen campylobacter jejuni is primarily transmitted via the consumption of contaminated foodstuffs, especially poultry meat. in food processing environments, c. jejuni is required to survive a multitude of stresses and requires the use of specific survival mechanisms, such as biofilms. an initial step in biofilm formation is bacterial attachment to a surface. here, we investigated the effects of a chicken meat exudate (chicken juice) on c. jejuni surface attachment and biofilm f ... | 2014 | 25192991 |
| dynamics of dual infection with campylobacter jejuni strains in chickens reveals distinct strain-to-strain variation in infection ecology. | although multiple genotypes of campylobacter jejuni may be isolated from the same commercial broiler flock, little is known about the infection dynamics of different genotypes within individuals or their colonization sites within the gut. single experimental infections with c. jejuni m1 (sequence type 137, clonal complex 45) and c. jejuni 13126 (sequence type 21, clonal complex 21) revealed that 13126 colonized the ceca at significantly higher levels. the dissemination and colonization sites of ... | 2014 | 25107966 |
| lactobacillus gasseri sbt2055 reduces infection by and colonization of campylobacter jejuni. | campylobacter is a normal inhabitant of the chicken gut. pathogenic infection with this organism in humans is accompanied by severe inflammation of the intestinal mucosal surface. the aim of this study was to evaluate the ability of lactobacillus gasseri sbt2055 (lg2055) to inhibit the adhesion and invasion of campylobacter jejuni in vitro and to suppress c. jejuni colonization of chicks in vivo. pretreatment with lg2055 significantly reduced adhesion to and invasion of a human epithelial cell l ... | 2014 | 25264604 |
| genome analysis of campylobacter jejuni strains isolated from a waterborne outbreak. | waterborne campylobacter jejuni outbreaks are common in the nordic countries, and pfge (pulsed field gel electrophoresis) remains the genotyping method of choice in outbreak investigations. however, pfge cannot assess the clonal relationship between isolates, leading to difficulties in molecular epidemiological investigations. here, we explored the applicability of whole genome sequencing to outbreak investigation by re-analysing three c. jejuni strains (one isolated from water and two from pati ... | 2014 | 25196593 |
| complete genome sequence and annotation of a campylobacter jejuni strain, mtvdscj20, isolated from a naturally colonized farm-raised chicken. | campylobacter jejuni is a major cause of human food-borne illness, with contaminated poultry products serving as a main source of human infection. c. jejuni strain mtvdscj20 was isolated from the cecal contents of a farm-raised chicken that was naturally colonized with campylobacter. we present here the complete annotated genome sequence of mtvdscj20. | 2014 | 25146148 |
| campylobacter jejuni is not merely a commensal in commercial broiler chickens and affects bird welfare. | campylobacter jejuni is the leading cause of bacterial food-borne infection; chicken meat is its main source. c. jejuni is considered commensal in chickens based on experimental models unrepresentative of commercial production. here we show that the paradigm of campylobacter commensalism in the chicken is flawed. through experimental infection of four commercial breeds of broiler chickens, we show that breed has a significant effect on c. jejuni infection and the immune response of the animals, ... | 2014 | 24987092 |
| the interplay between campylobacter and helicobacter species and other gastrointestinal microbiota of commercial broiler chickens. | poultry represent an important source of foodborne enteropathogens, in particular thermophilic campylobacter species. many of these organisms colonize the intestinal tract of broiler chickens as harmless commensals, and therefore, often remain undetected prior to slaughter. the exact reasons for the lack of clinical disease are unknown, but analysis of the gastrointestinal microbiota of broiler chickens may improve our understanding of the microbial interactions with the host. | 2014 | 24940386 |
| identification of possible virulence marker from campylobacter jejuni isolates. | a novel protein translocation system, the type-6 secretion system (t6ss), may play a role in virulence of campylobacter jejuni. we investigated 181 c. jejuni isolates from humans, chickens, and environmental sources in vietnam, thailand, pakistan, and the united kingdom for t6ss. the marker was most prevalent in human and chicken isolates from vietnam. | 2014 | 24856088 |
| campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation. | according to merops peptidase database, campylobacter species encode 64 predicted peptidases. however, proteolytic properties of only a few of these proteins have been confirmed experimentally. in this study we identified and characterised a campylobacter jejuni gene cj0511 encoding a novel peptidase. the proteolytic activity associated with this enzyme was demonstrated in cell lysates. moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine pept ... | 2014 | 24918062 |
| extensive microbial and functional diversity within the chicken cecal microbiome. | chickens are major source of food and protein worldwide. feed conversion and the health of chickens relies on the largely unexplored complex microbial community that inhabits the chicken gut, including the ceca. we have carried out deep microbial community profiling of the microbiota in twenty cecal samples via 16s rrna gene sequences and an in-depth metagenomics analysis of a single cecal microbiota. we recovered 699 phylotypes, over half of which appear to represent previously unknown species. ... | 2014 | 24657972 |
| passive immunization to reduce campylobacter jejuni colonization and transmission in broiler chickens. | campylobacter jejuni is the most common cause of bacterium-mediated diarrheal disease in humans worldwide. poultry products are considered the most important source of c. jejuni infections in humans but to date no effective strategy exists to eradicate this zoonotic pathogen from poultry production. here, the potential use of passive immunization to reduce campylobacter colonization in broiler chicks was examined. for this purpose, laying hens were immunized with either a whole-cell lysate or th ... | 2014 | 24589217 |
| functional characterization of exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (ppx/gppa) of campylobacter jejuni. | the inorganic polyphosphate (poly-p) is a key regulator of stress responses and virulence in many bacterial pathogens including campylobacter jejuni. the role of exopolyphosphatases/guanosine pentaphosphate (pppgpp) phosphohydrolases (ppx/gppa) in poly-p homeostasis and c. jejuni pathobiology remains unexplored. here, we analyzed deletion mutants (∆ppx1, ∆ppx2) and the double knockout mutant (dkppx), all ∆ppx mutants exhibited increased capacity to accumulate poly-p; however only ∆ppx1 and dkppx ... | 2014 | 24569519 |
| genome sequences of campylobacter jejuni 81-176 variants with enhanced fitness relative to the parental strain in the chicken gastrointestinal tract. | campylobacter jejuni is a major cause of food-borne infections in the united states due to its ability to asymptomatically colonize the gastrointestinal tracts of chickens. using competition assays with parental c. jejuni 81-176, variants with consistently improved fitness in chicken ceca relative to the parental strain were identified and sequenced. | 2014 | 24503981 |
| effect of noradrenaline on the virulence properties of campylobacter species. | campylobacter species cause a spectrum of illnesses in humans. the type of illness and the outcome is dependent on the virulence of the infecting pathogen strain and host immune status. acute stress can seriously compromise host immunity and increase susceptibility to infection. noradrenaline (na) is a stress hormone. several studies have shown that it stimulated growth and increased the pathogenicity of organisms including e. coli and campylobacter jejuni. however, the effect of na on other cam ... | 2014 | 24669220 |
| a novel o-linked glycan modulates campylobacter jejuni major outer membrane protein-mediated adhesion to human histo-blood group antigens and chicken colonization. | campylobacter jejuni is an important cause of human foodborne gastroenteritis; strategies to prevent infection are hampered by a poor understanding of the complex interactions between host and pathogen. previous work showed that c. jejuni could bind human histo-blood group antigens (bgags) in vitro and that bgags could inhibit the binding of c. jejuni to human intestinal mucosa ex vivo. here, the major flagella subunit protein (flaa) and the major outer membrane protein (momp) were identified as ... | 2014 | 24451549 |
| superoxide dismutase sodb is a protective antigen against campylobacter jejuni colonisation in chickens. | campylobacter is the leading cause of foodborne diarrhoeal illness in the developed world and consumption or handling of contaminated poultry meat is the principal source of infection. strategies to control campylobacter in broilers prior to slaughter are urgently required and are predicted to limit the incidence of human campylobacteriosis. towards this aim, a purified recombinant subunit vaccine based on the superoxide dismutase (sodb) protein of c. jejuni m1 was developed and tested in white ... | 2015 | 26458797 |
| high prevalence of hyper-aerotolerant campylobacter jejuni in retail poultry with potential implication in human infection. | campylobacter jejuni is a leading cause of foodborne illnesses around the world. since c. jejuni is microaerophilic and sensitive to oxygen, aerotolerance is important in the transmission of c. jejuni to humans via foods under aerobic conditions. in this study, 70 c. jejuni strains were isolated from retail raw chicken meats and were subject to multilocus sequence typing (mlst) analysis. in the aerotolerance testing by aerobic shaking at 200 rpm, 50 (71.4%) isolates survived after 12 h (i.e., ae ... | 2015 | 26617597 |
| heterogeneity in the infection biology of campylobacter jejuni isolates in three infection models reveals an invasive and virulent phenotype in a st21 isolate from poultry. | although campylobacter is the leading cause of bacterial foodborne gastroenteritis in the world and the importance of poultry as a source of infection is well understood we know relatively little about its infection biology in the broiler chicken. much of what we know about the biology of campylobacter jejuni is based on infection of inbred or spf laboratory lines of chickens with a small number of isolates used in most laboratory studies. recently we have shown that both the host response and m ... | 2015 | 26496441 |
| draft genome sequence of the enteropathogenic bacterium campylobacter jejuni strain cj255. | the enteropathogen campylobacter jejuni is a global health disaster, being one of the leading causes of bacterial gastroenteritis. here, we present the draft genome sequence of c. jejuni strain cj255, isolated from a chicken source in islamabad, pakistan. the draft genome sequence will aid in epidemiological studies and quarantine of this broad-host-range pathogen. | 2015 | 26494669 |
| population diversity of campylobacter jejuni in poultry and its dynamic of contamination in chicken meat. | this study aimed to analyse the diversity of the campylobacter jejuni population in broilers and to evaluate the major source of contamination in poultry meat. eight rearing cycles over one year provided samples from three different broiler farms processed at the same slaughterhouse. a total of 707 c. jejuni were isolated from cloacal swabs before slaughter and from the breast skin of carcasses after slaughter and after chilling. all suspected campylobacter colonies were identified with pcr assa ... | 2015 | 26543870 |
| development of multiple-locus variable-number tandem-repeat analysis for molecular subtyping of campylobacter jejuni by using capillary electrophoresis. | campylobacter jejuni is a common cause of the frequently reported food-borne diseases in developed and developing nations. this study describes the development of multiple-locus variable-number tandem-repeat (vntr) analysis (mlva) using capillary electrophoresis as a novel typing method for microbial source tracking and epidemiological investigation of c. jejuni. among 36 tandem repeat loci detected by the tandem repeat finder program, 7 vntr loci were selected and used for characterizing 60 iso ... | 2015 | 26025899 |
| chicken caecal microbiome modifications induced by campylobacter jejuni colonization and by a non-antibiotic feed additive. | campylobacter jejuni is an important zoonotic foodborne pathogen causing acute gastroenteritis in humans. chickens are often colonized at very high numbers by c. jejuni, up to 10(9) cfu per gram of caecal content, with no detrimental effects on their health. farm control strategies are being developed to lower the c. jejuni contamination of chicken food products in an effort to reduce human campylobacteriosis incidence. it is believed that intestinal microbiome composition may affect gut coloniz ... | 2015 | 26161743 |
| genetic basis and functional consequences of differential expression of the cmeabc efflux pump in campylobacter jejuni isolates. | the cmeabc multidrug efflux transporter of campylobacter jejuni plays a key role in antimicrobial resistance and is suppressed by cmer, a transcriptional regulator of the tetr family. overexpression of cmeabc has been observed in laboratory-generated mutants, but it is unknown if this phenotype occurs naturally in c. jejuni isolates and if it has any functional consequences. to answer these questions, expression of cmeabc in natural isolates obtained from broiler chickens, turkeys and humans was ... | 2015 | 26132196 |
| evaluation of a polysaccharide conjugate vaccine to reduce colonization by campylobacter jejuni in broiler chickens. | campylobacter jejuni is a leading bacterial cause of food-borne illness in humans. symptoms range from mild gastroenteritis to dysentery. contaminated chicken meat is the most common cause of infection. broiler chickens become colonized with high numbers of c. jejuni in the intestinal tract, but do not become clinically ill. vaccination of broiler chicks to control colonization by c. jejuni is challenging because immune function is limited in the first 2 weeks post-hatch and immune suppressive m ... | 2015 | 26032784 |
| transducer like proteins of campylobacter jejuni 81-176: role in chemotaxis and colonization of the chicken gastrointestinal tract. | transducer like proteins (tlps), also known as methyl accepting chemotaxis proteins (mcp), enable enteric pathogens to respond to changing nutrient levels in the environment by mediating taxis toward or away from specific chemoeffector molecules. despite recent advances in the characterization of chemotaxis responses in campylobacter jejuni, the impact of tlps on the adaptation of this pathogen to disparate niches and hosts is not fully characterized. the latter is particularly evident in the ca ... | 2015 | 26075188 |
| extensive characterization of campylobacter jejuni chicken isolates to uncover genes involved in the ability to compete for gut colonization. | campylobacter jejuni is responsible for human foodborne enteritis. this bacterium is a remarkable colonizer of the chicken gut, with some strains outcompeting others for colonization. to better understand this phenomenon, the objective of this study was to extensively characterize the phenotypic performance of c. jejuni chicken strains and associate their gut colonizing ability with specific genes. | 2015 | 25958385 |
| predominant campylobacter jejuni sequence types persist in finnish chicken production. | consumption and handling of chicken meat are well-known risk factors for acquiring campylobacteriosis. this study aimed to describe the campylobacter jejuni population in finnish chickens and to investigate the distribution of c. jejuni genotypes on finnish chicken farms over a period of several years. we included 89.8% of the total c. jejuni population recovered in finnish poultry during 2004, 2006, 2007, 2008, and 2012 and used multilocus sequence typing (mlst) and pulsed-field gel electrophor ... | 2015 | 25700264 |
| age-related differences in the luminal and mucosa-associated gut microbiome of broiler chickens and shifts associated with campylobacter jejuni infection. | despite the importance of gut microbiota for broiler performance and health little is known about the composition of this ecosystem, its development and response towards bacterial infections. therefore, the current study was conducted to address the composition and structure of the microbial community in broiler chickens in a longitudinal study from day 1 to day 28 of age in the gut content and on the mucosa. additionally, the consequences of a campylobacter (c.) jejuni infection on the microbia ... | 2016 | 27921008 |
| lipooligosaccharide locus classes and putative virulence genes among chicken and human campylobacter jejuni isolates. | campylobacter cause morbidity and considerable economic loss due to hospitalization and post infectious sequelae such as reactive arthritis, guillain barré- and miller fischer syndromes. such sequelae have been linked to c. jejuni harboring sialic acid structures in their lipooligosaccharide (los) layer of the cell wall. poultry is an important source of human campylobacter infections but little is known about the prevalence of sialylated c. jejuni isolates and the extent of transmission of such ... | 2016 | 27871232 |
| the pas domain-containing protein heur regulates heme uptake in campylobacter jejuni. | campylobacter jejuni is a leading cause of bacterially derived gastroenteritis. a previous mutant screen demonstrated that the heme uptake system (chu) is required for full colonization of the chicken gastrointestinal tract. subsequent work identified a pas domain-containing regulator, termed heur, as being required for chicken colonization. here we confirm that both the heme uptake system and heur are required for full chicken gastrointestinal tract colonization, with the heur mutant being part ... | 2016 | 27935836 |
| differences in host breed and diet influence colonization by campylobacter jejuni and induction of local immune responses in chicken. | chickens are regarded as the main reservoir for human campylobacteriosis. little is known about the interaction between campylobacter jejuni (c. jejuni) and chickens. this interaction may be influenced by the stage of maturation of the immune system, developing gut microbiota composition and other factors including breed and diet. our aim was to investigate the impact of breed, and diet on c. jejuni colonization and host immune responses in chickens. birds were inoculated with 10(4) colony formi ... | 2016 | 27843492 |
| chicken immune response after in ovo immunization with chimeric tlr5 activating flagellin of campylobacter jejuni. | campylobacter jejuni is the main cause of bacterial food-borne diseases in developed countries. chickens are the most important source of human infection. vaccination of poultry is an attractive strategy to reduce the number of c. jejuni in the intestinal tract of chickens. we investigated the immunogenicity and protective efficacy of a recombinant c. jejuni flagellin-based subunit vaccine with intrinsic adjuvant activity. toll-like receptor activation assays demonstrated the purity and tlr5 sti ... | 2016 | 27760175 |
| virulence and genomic feature of multidrug resistant campylobacter jejuni isolated from broiler chicken. | the aim of this study was to reveal the molecular mechanism involved in multidrug resistance and virulence of campylobacter jejuni isolated from broiler chickens. the virulence of six multidrug resistant c. jejuni was determined by in vitro and in vivo methods. the de novo whole genome sequencing technology and molecular biology methods were used to analyze the genomic features associated with the multidrug resistance and virulence of a selected isolate (c. jejuni 1655). the comparative genomic ... | 2016 | 27790202 |
| complete genomic sequence of campylobacter jejuni subsp. jejuni hs:19 strain rm1285 isolated from packaged chicken. | poultry products serve as the main source of campylobacter jejuni subsp. jejuni infections in humans. c. jejuni subsp. jejuni infections are a leading cause of foodborne gastroenteritis and are a prevalent antecedent to guillain-barré syndrome. this study describes the genome of c. jejuni subsp. jejuni hs:19 strain rm1285, isolated from packaged chicken in california. | 2016 | 27795263 |
| complete genome sequences of campylobacter jejuni strains od267 and wp2202 isolated from retail chicken livers and gizzards reveal the presence of novel 116-kilobase and 119-kilobase megaplasmids with type vi secretion systems. | genome sequences of campylobacter jejuni strains od267 and wp2202, isolated from chicken livers and gizzards, showed the presence of novel 116-kb and 119-kb megaplasmids, respectively. the two megaplasmids carry a type vi secretion system and tetracycline resistance genes. these are the largest sequenced campylobacter plasmids to date. | 2016 | 27688318 |
| dna supercoiling regulates the motility of campylobacter jejuni and is altered by growth in the presence of chicken mucus. | campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans, but relatively little is known about the global regulation of virulence factors during infection of chickens or humans. this study identified dna supercoiling as playing a key role in regulating motility and flagellar protein production and found that this supercoiling-controlled regulon is induced by growth in chicken mucus. a direct correlation was observed between motility and resting dna supercoiling levels in ... | 2016 | 27624126 |
| restaurant cooking trends and increased risk for campylobacter infection. | in the united kingdom, outbreaks of campylobacter infection are increasingly attributed to undercooked chicken livers, yet many recipes, including those of top chefs, advocate short cooking times and serving livers pink. during 2015, we studied preferences of chefs and the public in the united kingdom and investigated the link between liver rareness and survival of campylobacter. we used photographs to assess chefs' ability to identify chicken livers meeting safe cooking guidelines. to investiga ... | 2016 | 27314748 |
| detection of zoonotic enteropathogens in children and domestic animals in a semirural community in ecuador. | animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (lmics), where small-scale livestock production is common. in this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in ecuador between june and august 2014. multilocus sequence typing (mlst) was used to assess zoonotic tran ... | 2016 | 27208122 |
| risk factors associated with campylobacter detected by pcr in humans and animals in rural cambodia. | campylobacter are worldwide-occurring zoonotic bacteria, with the species campylobacter jejuni and c. coli commonly associated with diarrhoea in children in low-income countries. in this cross-sectional study, the prevalence of c. jejuni and c. coli in human and livestock faecal samples was detected by pcr and zoonotic risk factors associated with human campylobacter positivity were identified. in total 681 humans and 753 livestock (chickens, ducks, pigs, cattle) from 269 households were sampled ... | 2016 | 27334412 |
| campylobacter jejuni-associated perimyocarditis: two case reports and review of the literature. | campylobacter spp. are among the most common bacterial causes of gastroenteritis world-wide and mostly follow a benign course. we report two cases of campylobacter jejuni-associated perimyocarditis, the first two simultaneous cases published to date and the third and fourth cases over all in sweden, and a review of the literature. | 2016 | 27297408 |
| tracing back clinical campylobacter jejuni in the northwest of italy and assessing their potential source. | food-borne campylobacteriosis is caused mainly by the handling or consumption of undercooked chicken meat or by the ingestion of contaminated raw milk. knowledge about the contributions of different food sources to gastrointestinal disease is fundamental to prioritize food safety interventions and to establish proper control strategies. assessing the genetic diversity among campylobacter species is essential to our understanding of their epidemiology and population structure. we molecularly char ... | 2016 | 27379033 |
| prevalence of virulence/stress genes in campylobacter jejuni from chicken meat sold in qatari retail outlets. | chicken meat from the shelves of supermarkets in qatar was tested for the presence of campylobacter spp. and the presence of five virulence genes (htrb, cdtb, clpp, cadf and ciab) was assessed in isolates. forty eight percent of the chickens provided for supermarkets by saudi (53%) and qatari (45.9%) producers were found to be contaminated and the most important factor affecting the overall prevalence of contaminated chickens was the store from which chicken samples originated. variation in prev ... | 2016 | 27258021 |
| recent advances in screening of anti-campylobacter activity in probiotics for use in poultry. | campylobacteriosis is the most common cause of bacterial gastroenteritis worldwide. campylobacter species involved in this infection usually include the thermotolerant species campylobacter jejuni. the major reservoir for c. jejuni leading to human infections is commercial broiler chickens. poultry flocks are frequently colonized by c. jejuni without any apparent symptoms. risk assessment analyses have identified the handling and consumption of poultry meat as one of the most important sources o ... | 2016 | 27303366 |
| engineering the campylobacter jejuni n-glycan to create an effective chicken vaccine. | campylobacter jejuni is a predominant cause of human gastroenteritis worldwide. source-attribution studies indicate that chickens are the main reservoir for infection, thus elimination of c. jejuni from poultry would significantly reduce the burden of human disease. we constructed glycoconjugate vaccines combining the conserved c. jejuni n-glycan with a protein carrier, glycotag, or fused to the escherichia coli lipopolysaccharide-core. vaccination of chickens with the protein-based or e. coli-d ... | 2016 | 27221144 |
| chicken anti-campylobacter vaccine - comparison of various carriers and routes of immunization. | campylobacter spp, especially the species campylobacter jejuni, are important human enteropathogens responsible for millions of cases of gastro-intestinal disease worldwide every year. c. jejuni is a zoonotic pathogen, and poultry meat that has been contaminated by microorganisms is recognized as a key source of human infections. although numerous strategies have been developed and experimentally checked to generate chicken vaccines, the results have so far had limited success. in this study, we ... | 2016 | 27242755 |
| biochar, bentonite and zeolite supplemented feeding of layer chickens alters intestinal microbiota and reduces campylobacter load. | a range of feed supplements, including antibiotics, have been commonly used in poultry production to improve health and productivity. alternative methods are needed to suppress pathogen loads and maintain productivity. as an alternative to antibiotics use, we investigated the ability of biochar, bentonite and zeolite as separate 4% feed additives, to selectively remove pathogens without reducing microbial richness and diversity in the gut. neither biochar, bentonite nor zeolite made any signific ... | 2016 | 27116607 |
| glycoengineered outer membrane vesicles: a novel platform for bacterial vaccines. | the world health organization has indicated that we are entering into a post-antibiotic era in which infections that were routinely and successfully treated with antibiotics can now be lethal due to the global dissemination of multidrug resistant strains. conjugate vaccines are an effective way to create a long-lasting immune response against bacteria. however, these vaccines present many drawbacks such as slow development, high price, and batch-to-batch inconsistencies. alternate approaches for ... | 2016 | 27103188 |
| the genomic architecture of resistance to campylobacter jejuni intestinal colonisation in chickens. | campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. in the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to campylobacter could potentially reduce its subsequent entry into the food chain. campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. in the present study, two chicken populations wer ... | 2016 | 27090510 |
| draft genome sequence of lactobacillus crispatus jcm5810, which can reduce campylobacter jejuni colonization in chicken intestine. | we present the 2.05-mb draft genome sequence oflactobacillus crispatusjcm5810, a chicken intestinal isolate with the ability to reducecampylobacter jejunicolonization in chickens. the genome sequence will provide insights on the probiotic mechanisms ofl. crispatusjcm5810. | 2016 | 27081134 |
| evaluation of flagellum-related proteins flid and fspa as subunit vaccines against campylobacter jejuni colonisation in chickens. | campylobacter is the leading cause of food-borne diarrhoea in humans in the developed world and consumption of contaminated poultry meat is the main source of infection. vaccination of broilers could reduce carcass contamination and zoonotic infections. towards this aim, we evaluated recombinant anti-campylobacter subunit vaccines based on the flagellum-capping protein flid and the flagellum-secreted protein fspa as they are immunogenic in chickens and the flagellum is vital for colonisation. in ... | 2016 | 26921781 |
| cytokine responses in birds challenged with the human food-borne pathogen campylobacter jejuni implies a th17 response. | development of process orientated understanding of cytokine interactions within the gastrointestinal tract during an immune response to pathogens requires experimentation and statistical modelling. the immune response against pathogen challenge depends on the specific threat to the host. here, we show that broiler chickens mount a breed-dependent immune response to campylobacter jejuni infection in the caeca by analysing experimental data using frequentist and bayesian structural equation models ... | 2016 | 27069644 |
| the efficacy of a commercial competitive exclusion product on campylobacter colonization in broiler chickens in a 5-week pilot-scale study. | the efficacy of the commercial competitive exclusion product broilact against campylobacter jejuni was evaluated in broiler chickens in a 5-week pilot-scale study. newly-hatched broiler chicks were brought from a commercial hatchery. after arrival 50 seeder chicks were challenged orally with approximately 10(3) cfu of c. jejuni, wing marked, and placed back in a delivery box and moved to a separate room. the rest of the chicks (contact chicks) were placed in floor pens, 100 chicks per pen. birds ... | 2016 | 26944963 |
| cell wall anchoring of the campylobacter antigens to lactococcus lactis. | campylobacter jejuni is the most frequent cause of human food-borne gastroenteritis and chicken meat is the main source of infection. recent studies showed that broiler chicken immunization against campylobacter should be the most efficient way to lower the number of human infections by this pathogen. induction of the mucosal immune system after oral antigen administration should provide protective immunity to chickens. in this work we tested the usefulness of lactococcus lactis, the most extens ... | 2016 | 26925040 |
| human campylobacteriosis in luxembourg, 2010-2013: a case-control study combined with multilocus sequence typing for source attribution and risk factor analysis. | campylobacteriosis has increased markedly in luxembourg during recent years. we sought to determine which campylobacter genotypes infect humans, where they may originate from, and how they may infect humans. multilocus sequence typing was performed on 1153 campylobacter jejuni and 136 c. coli human strains to be attributed to three putative animal reservoirs (poultry, ruminants, pigs) and to environmental water using the asymmetric island model. a nationwide case-control study (2010-2013) for do ... | 2016 | 26860258 |
| antibacterial, antiviral, and antifungal properties of wines and winery byproducts in relation to their flavonoid content. | grapes produce organic compounds that may be involved in the defense of the plants against invading phytopathogens. these metabolites include numerous phenolic compounds that are also active against human pathogens. grapes are used to produce a variety of wines, grape juices, and raisins. grape pomace, seeds, and skins, the remains of the grapes that are a byproduct of winemaking, also contain numerous bioactive compounds that differ from those found in grapes and wines. this overview surveys an ... | 2014 | 24945318 |
| purification and characterization of a novel anti-campylobacter bacteriocin produced by lactobacillus curvatus dn317. | the lactic acid bacteria (lab) microbiota of saudi chicken ceca was determined. from 60 samples, 204 isolates of lactic acid bacteria were obtained. three isolates produced antimicrobial activities against campylobacter jejuni, listeria monocytogenes, and bacillus subtilis. the isolate dn317, which had the highest activity against campylobacter jejuni atcc 33560, was identified as lactobacillus curvatus (genbank accession numbers: kx353849 and kx353850). full inhibitory activity was observed aft ... | 2016 | 27812926 |
| targeting motility properties of bacteria in the development of probiotic cultures against campylobacter jejuni in broiler chickens. | campylobacter is the leading cause of gastroenteritis worldwide. campylobacter is commonly present in the intestinal tract of poultry, and one strategy to reduce enteric colonization is the use of probiotic cultures. this strategy has successfully reduced enteric colonization of salmonella, but has had limited success against campylobacter. in an effort to improve the efficacy of probiotic cultures, we developed a novel in vitro screening technique for selecting bacterial isolates with enhanced ... | 2013 | 23531121 |
| application of protective cultures against listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere. | to control the growth, or reduce the numbers, of food pathogens such as listeria monocytogenes and campylobacter jejuni in chicken products packaged under modified atmosphere (map), the effectiveness of protective cultures was evaluated in this study. leuconostoc pseudomesenteroides pck18 reduced the counts of l. monocytogenes by 1.22 log cfu/g in chicken burgers under map after 24 d. furthermore, a reduction of 1.16 log cfu/g in c. jejuni together with a delay in the growth of lactic acid bacte ... | 2013 | 23472035 |
| occurrence of pathogens in wild rodents caught on swedish pig and chicken farms. | a total of 207 wild rodents were caught on nine pig farms, five chicken farms and five non-farm locations in sweden and surveyed for a selection of bacteria, parasites and viruses. lawsonia intracellularia and pathogenic yersinia enterocolitica were only detected in rodents on pig farms (9% and 8% prevalence, respectively) which indicate that these agents are more likely to be transmitted to rodents from pigs or the environment on infected farms. brachyspira hyodysenteriae (1%), brachyspira inte ... | 2013 | 23174339 |
| further characterization and independent validation of a dna aptamer-quantum dot-based magnetic sandwich assay for campylobacter. | previously reported dna aptamers developed against surface proteins extracted from campylobacter jejuni were further characterized by aptamer-based western blotting and shown to bind epitopes on proteins weighing ~16 and 60 kd from reduced c. jejuni and campylobacter coli lysates. proteins of these approximate weights have also been identified in traditional antibody-based western blots of campylobacter spp. specificity of the capture and reporter aptamers from the previous report was further va ... | 2017 | 28342148 |
| variation in campylobacter multilocus sequence typing subtypes from chickens as detected on three plating media. | the objective of this study was to compare subtypes of campylobacter jejuni and campylobacter coli detected on three selective campylobacter plating media to determine whether each medium selected for different subtypes. fifty ceca and 50 carcasses (representing 50 flocks) were collected from the evisceration line in a commercial broiler processing plant. campylobacter was cultured and isolated from cecal contents and carcass rinses on campy-cefex, campy line, and rf campylobacter jejuni/coli ag ... | 2016 | 28221919 |
| genetic diversity and antimicrobial resistance profiles of campylobacter coli and campylobacter jejuni isolated from broiler chicken in farms and at time of slaughter in central italy. | genetic diversity and antimicrobial resistance of campylobacter coli and campylobacter jejuni were investigated along the broiler chicken production chain in central italy. | 2017 | 28194914 |
| prevalence of thermotolerant campylobacter spp. in chicken meat in croatia and multilocus sequence typing of a small subset of campylobacter jejuni and campylobacter coli isolates. | in order to detect thermotolerant campylobacter spp., 241 samples of fresh chicken meat, at retail in croatia, were analysed according to a standard method, followed by biochemical test and molecular polymerase chain reaction/restriction enzyme analysis for exact species determination. campylobacter spp. prevalence was 73.86%. campylobacter jejuni and campylobacter coli were isolated from 53.53 and 15.35% of the samples, respectively. in 4.98% of isolates thermotolerant campylobacter spp. were n ... | 2016 | 28115906 |
| sensitivity of direct culture, enrichment and pcr for detection of campylobacter jejuni and c. coli in broiler flocks at slaughter. | broiler chicken flocks are a significant source of campylobacter jejuni and campylobacter coli that result in the major public health problem of campylobacteriosis. accurate estimates of the prevalence of both c. coli and c. jejuni in flocks would enhance epidemiological understanding, risk assessment and control options. this study combined results from a panel of 10 detection tests (direct culture, enrichment and pcr) on caecal samples from flocks at slaughter. a parallel interpretation approa ... | 2016 | 27770505 |
| occurrence and genotypes of campylobacter species in broilers during the rearing period. | poultry are the main source of campylobacter infection worldwide. to obtain information on campylobacter-infected flocks and create a reference for preventing and controlling campylobacter at farm level, campylobacter isolates were recovered from broilers and the environments of nine chicken flocks in two farms during growth. the genetic relationship between the campylobacter isolates was determined using multilocus sequence typing. flocks were colonized as early as 3 weeks after introduction to ... | 2017 | 27766896 |
| sensitivity to enterocins of thermophilic campylobacter spp. from different poultry species. | campylobacter spp. have been isolated from various animals, including poultry. they are rapidly transmitted throughout broiler sheds by the fecal-oral route. a promising strategy to reduce campylobacter spp. in poultry may be done due to the beneficial properties of probiotic bacteria and their bacteriocins. in this study, inhibition spectrum/activity of different enterocins was evaluated against campylobacter spp. (isolated from different poultry) to indicate further practical use of enterocins ... | 2016 | 27602434 |
| macrolide resistance mechanisms and virulence factors in erythromycin-resistant campylobacter species isolated from chicken and swine feces and carcasses. | resistance to antimicrobials was measured in 73 isolates of campylobacter jejuni (c. jejuni) and 121 isolates of campylobacter coli (c. coli) from chicken and swine feces and carcasses in korea. both bacterial species showed the highest resistance to (fluoro) quinolones (ciprofloxacin and nalidixic acid) out of the nine antimicrobials tested. erythromycin resistance was much higher in c. coli (19.0%, 23/121) than in c. jejuni (6.8%, 5/73). the mutation in the 23s rrna gene was primarily responsi ... | 2017 | 27593510 |
| combined campylobacter jejuni and campylobacter coli rapid testing and molecular epidemiology in conventional broiler flocks. | campylobacter spp. are important causes of bacterial zoonosis, most often transmitted by contaminated poultry meat. from an epidemiological and risk assessment perspective, further knowledge should be obtained on campylobacter prevalence and genotype distribution in primary production. consequently, 15 austrian broiler flocks were surveyed in summer for their thermophilic campylobacter spp. contamination status. chicken droppings, dust and drinking water samples were collected from each flock at ... | 2016 | 27061601 |
| the effects of 405-nm visible light on the survival of campylobacter on chicken skin and stainless steel. | campylobacter spp. are foodborne pathogens responsible for a significant portion of human cases of bacterial-mediated gastrointestinal disease. a primary method for the introduction of campylobacter into the food supply is through poultry products. reducing the number of campylobacter on poultry products may reduce the incidence of human disease. research has been conducted on the use of light to inactivate campylobacter on poultry products and processing environments. more recently, the use of ... | 2016 | 26938455 |
| zoonotic public health hazards in backyard chickens. | backyard poultry has become increasingly popular in industrialized countries. in addition to keeping chickens for eggs and meat, owners often treat the birds as pets. however, several pathogenic enteric bacteria have the potential for zoonotic transmission from poultry to humans but very little is known about the occurrence of zoonotic pathogens in backyard flocks. the occurrence and the antimicrobial resistance of salmonella enterica, campylobacter spp., listeria monocytogenes and enteropathoge ... | 2016 | 26752227 |
| salmonella and campylobacter: antimicrobial resistance and bacteriophage control in poultry. | salmonella and campylobacter are major causes of foodborne related illness and are traditionally associated with consuming undercooked poultry and/or consuming products that have been cross contaminated with raw poultry. many of the isolated salmonella and campylobacter that can cause disease have displayed antimicrobial resistance phenotypes. although poultry producers have reduced on-the-farm overuse of antimicrobials, antimicrobial resistant salmonella and campylobacter strains still persist. ... | 2016 | 26678136 |
| comprehensive analysis of flagellin glycosylation in campylobacter jejuni nctc 11168 reveals incorporation of legionaminic acid and its importance for host colonization. | campylobacter jejuni is the leading cause of bacterial gastroenteritis. it relies on several virulence factors for host colonization, including glycosylated flagella. c. jejuni nctc 11168 modifies its flagellins with pseudaminic acid derivatives. it is also presumed to modify these proteins with legionaminic acid, although no glycopeptide evidence was available at the onset of this study. the enzyme encoded by cj1319 can be used to make legionaminic acid in vitro, but the pathway for legionamini ... | 2016 | 26582606 |
| species shift and multidrug resistance of campylobacter from chicken and swine, china, 2008-14. | the objective of this study was to investigate the prevalence and antimicrobial resistance of campylobacter isolated from broiler chickens and swine during 2008-14. | 2016 | 26568567 |
| [antimicrobial susceptibility and resistance mutations in campylobacter jejuni and c. coli isolates from human and meat sources]. | recently, there has been a marked increase in the number of reports of fluoroquinolone-resistant campylobacter jejuni and campylobacter coli. the aim of this study was to evaluate the prevalence of antimicrobial resistance and its genetic determinants in campylobacter species isolated from meat and human subjects in fukuoka prefecture, japan. between 2011 and 2013, 55 and 64 isolates were collected from meat (chicken meat and beef liver) and humans, respectively, in this prefecture. antimicrobia ... | 2015 | 26552121 |
| risk of escherichia coli o157:h7, non-o157 shiga toxin-producing escherichia coli, and campylobacter spp. in food animals and their products in qatar. | escherichia coli o157:h7, non-o157 e. coli, and campylobacter spp. are among the top-ranked pathogens that threaten the safety of food supply systems around the world. the associated risks and predisposing factors were investigated in a dynamic animal population using a repeat-cross-sectional study design. animal and environmental samples were collected from dairy and camel farms, chicken processing plants, and abattoirs and analyzed for the presence of these pathogens using a combination of bac ... | 2015 | 26408129 |
| rapid detection of campylobacter jejuni, campylobacter coli, and campylobacter lari in fresh chicken meat and by-products in bangkok, thailand, using modified multiplex pcr. | a multiplex pcr assay for simultaneous detection and differentiation of campylobacter jejuni, campylobacter coli, and campylobacter lari was developed and validated to assess the occurrence of these bacteria in fresh chicken meat and by-products in bangkok, thailand, by using a new combination of four previously published pcr primers for c. jejuni, c. coli, c. lari, and a universal 16s rdna gene as an internal control. the specificity was determined by using 13 strains of other bacteria. with pu ... | 2015 | 26197289 |
| comparative analysis of antimicrobial resistance and genetic diversity of campylobacter from broilers slaughtered in poland. | in the current study, the relationship of campylobacter jejuni and campylobacter coli strains isolated at slaughter was investigated using comparative analysis of antimicrobial resistance (amr), virulence gene (vg) and pfge profiling. a total of 254 campylobacter isolates from poultry caeca and corresponding carcasses, including 139 c. jejuni and 115 c. coli strains were tested. the most prevalent resistance profiles observed in c. jejuni were ciprofloxacin, nalidixic acid and tetracycline (46 o ... | 2015 | 26092707 |
| prevalence of campylobacter spp. in retail chicken, turkey, pork, and beef meat in poland between 2009 and 2013. | the purpose of the present study was to determine the prevalence of thermophilic campylobacter in poultry, pork, and beef meat at the retail level and to identify the main categories of meat representing the most significant reservoirs of campylobacter. a monitoring study was conducted throughout poland from 2009 to 2013. a total of 1,700 fresh meat samples were collected from supermarkets, large retail outlets, and smaller stores. thermophilic campylobacter species were detected in 690 (49.3%) ... | 2015 | 25951401 |
| higher resistance of campylobacter coli compared to campylobacter jejuni at chicken slaughterhouse. | in order to compare the prevalence of campylobacter coli and campylobacter jejuni during the processing of broilers at slaughterhouse a total of 848 samples were analyzed during 2012 in southern spain. four hundred and seventy six samples were collected from cloaca, carcass surfaces and quartered carcasses. moreover, 372 environmental swabs from equipment and scalding water were collected. minimum inhibitory concentration (mic) to ciprofloxacin, erythromycin, streptomycin, tetracycline and genta ... | 2015 | 25770597 |
| novel gentamicin resistance genes in campylobacter isolated from humans and retail meats in the usa. | to understand the molecular epidemiology of gentamicin-resistant campylobacter and investigate aminoglycoside resistance mechanisms. | 2015 | 25645207 |
| rapid identification and classification of campylobacter spp. using laser optical scattering technology. | campylobacter jejuni and campylobacter coli are the two important species responsible for most of the campylobacter infections in humans. reliable isolation and detection of campylobacter spp. from food samples are challenging due to the interferences from complex food substances and the fastidious growth requirements of this organism. in this study, a novel biosensor-based detection called bardot (bacterial rapid detection using optical scattering technology) was developed for high-throughput s ... | 2015 | 25583335 |
| [enumeration and antimicrobial resistance of campylobacter species from retail chicken carcasses]. | to determine campylobacter contamination level and antimicrobial resistance patterns from chicken carcasses in supermarkets and farmer's markets of 9 districts in beijing. | 2014 | 25573127 |
| seasonality and antibiotic resistance of campylobacter in turkish chicken meat. | this study investigated the seasonal prevalence and the antimicrobial susceptibility of campylobacter jejuni and campylobacter coli in 264 samples of chicken meat. the samples encompassed wings (n=88), breasts (n=79) and thighs (n=97) and were purchased from different butcheries and markets in elazig province, in eastern turkey, between december 2009 and november 2010. the meat samples were tested for campylobacter presence and the collected isolates were identified as campylobacter jejuni and c ... | 2014 | 25546065 |
| genotypes and antibiotic resistance of bovine campylobacter and their contribution to human campylobacteriosis. | campylobacter jejuni and campylobacter coli are the most important bacterial causes of human gastroenteritis. chicken has been recognized as a major source for human infection, whereas cattle might also contribute to a lesser extent. however, there is a paucity of information available regarding campylobacter in swiss cattle and their role for human campylobacteriosis. to gain more information on genotypes and antibiotic resistance of bovine c. jejuni and c. coli and on their contribution to hum ... | 2015 | 25511436 |
| characterization of campylobacter jejuni and campylobacter coli genotypes in poultry flocks by restriction fragment length polymorphism (rflp) analysis. | we describe a simple, rapid, and discriminatory methodology that allows the routine molecular characterization of campylobacter jejuni and campylobacter coli isolates. the proposed approach is built on one of the earliest and simplest molecular typing methods ever, consisting on the analysis of the fragments of different lengths generated by digestion of homologous dna sequences with specific restriction endonucleases, a process known as restriction fragment length polymorphism (rflp) analysis. ... | 2015 | 25399105 |
| multilocus sequence typing of campylobacter jejuni and campylobacter coli from humans and chickens in north-eastern italy. | this paper reports the multilocus sequence typing (mlst) of 57 c. jejuni and c. coli isolates from humans and chickens in italy and the identification of 17 new sequence types (sts). a high genetic diversity was detected among c. jejuni/c. coli and human/chicken isolates, with a predominance of clonal complexes cc21 and cc828. although human sts were not the same as those found in chickens, 3 ccs overlapped between human and chicken isolates. genotyping of campylobacter strains by mlst should be ... | 2014 | 25387294 |