| immunodiagnosis of human anisakiasis by use of larval excretory-secretory antigen. | l3 larvae of anisakis simplex, isolated from freshly caught herrings were cultured in medium 199 at different temperatures to obtain excretory-secretory (es) antigen. larvae have been cultured in vitro for a period of 11 months. a crude extract of a. simplex larvae was compared with es antigen obtained under 4 different culture conditions for the determination of igg and ige antibodies by elisa and a modified rast, respectively. 1 serum from a patient with histopathologically proved anisakiasis ... | 1989 | 2711789 |
| [anisakiasis (herring worm disease). pathway of transmission--clinical picture--therapeutic consequences]. | | 1989 | 2687676 |
| electrophoretic identification of anisakis sp. larvae (ascaridida: anisakidae) from clupea harengus l. in baltic sea. | on the basis of electrophoretic studies carried out on 15 gene-enzyme systems, 80 anisakis sp. larvae from the herring clupea harengus, fished in south baltic sea and gdanisk bay, were identified as anisakis simplex b. this is the first record of anisakis simplex b in the baltic sea. the spawning migration of the herrings from the north sea to the baltic sea and the distribution of a. simplex b are briefly discussed. | 1989 | 2487893 |
| [infestation by larvae of anisakis simplex a and anisakis physeteris in fish species of the italian seas]. | data on the occurrence of larvae of anisakis simplex a and anisakis physeteris in marine fishes from italian waters are reported. the larvae have been identified by multilocus electrophoresis using biochemical keys. considerations on the life-history pattern of these species in the mediterranean sea are advanced. | 1989 | 2487892 |
| a serodiagnostic assay by microenzyme-linked immunosorbent assay for human anisakiasis using a monoclonal antibody specific for anisakis larvae antigen. | a serodiagnostic assay system to detect human anisakiasis is described. this assay uses a microenzyme-linked immunosorbent assay with monoclonal antibody an2 that recognizes the antigen specifically found in anisakis simplex larvae. the data showed that sera from infected patients reacted strongly with the a. simplex larvae antigen that was immobilized with monoclonal antibody an2 on microplates. patients' igg, iga, igm, and ige reacted strongly with an2-defined antigen at 4 or 5 weeks and at 1 ... | 1990 | 2324550 |
| immunoblot analysis of serum igg, iga and ige responses against larval excretory-secretory antigens of anisakis simplex in patients with gastric anisakiasis. | to increase our understanding of the immune response to anisakis infection, antigen specific igg, iga and ige responses were identified using an immunoblot technique after polyacrylamide gel electrophoresis of excretory-secretory products from the larval stage of anisakis simplex. nine sera were drawn from proven cases of gastric anisakiasis within 3 days after symptoms had developed. the molecular weight of the major antigenic bands were distributed between 50 kda and 120 kda of the antigens. i ... | 1990 | 2283472 |
| serine proteases from nematode and protozoan parasites: isolation of sequence homologs using generic molecular probes. | serine proteases are one of the biologically most important and widely distributed families of enzymes. isolation of serine protease genes from organisms of widely diverged phylogenetic groups would provide a basis for studying their biological function, the relationship between structure and function, and the molecular evolution of these enzymes. serine proteases for which little structural information is known are those that are important in the pathogenesis of parasitic nematode and protozoan ... | 1989 | 2662185 |
| human anisakiasis transmitted by marine food products. | seafood-transmitted parasitic diseases represent an emerging area of interest to the u.s. food and drug administration. human infections with marine parasites are generally the result of ingesting uncooked seafood products. over 50 species of helminthic parasites are known to infect humans worldwide. recently, the number of infections with one of these helminths, the juvenile stage of the marine nematode, anisakis simplex, has increased in the united states. raw fish dishes such as lomi lomi sal ... | 1991 | 2022472 |
| [seroepidemiological data on anisakiasis: prophylactic consequences in fish products]. | the western blotting method allowed us to confirm the diagnosis in 7 cases of acute anisakiasis, to assess it in 5 cases of chronic anisakiasis, and to follow up the evolution. these chronic frames according to the question of their long term evolution, and the part played by anisakis simplex in fish nutritional allergy increase the negative score of that parasite. this situation should leave to discuss preventive rules. | 1991 | 1863866 |
| anisakis simplex, a co-factor of gastric cancer? | | 1990 | 1976977 |
| [geographic distribution and hosts of anisakis simplex and anisakis simplex b (ascaridida: anisakidae)]. | | 1986 | 3752809 |
| identification of the secreted neutral proteases from anisakis simplex. | ingestion of larval nematodes (family: anisakidae) can cause the human disease known as anisakiasis. after ingestion, anisakis larvae can be invasive, penetrating host stomach or intestinal wall. observation of larvae penetrating the tissue layers of human stomach in vitro by sem showed tunnels and burrows were formed in the mucosa and submucosa. based on these observations, we hypothesized that secreted proteases may be involved in the degradation of host tissue macromolecules to allow tunnel f ... | 1990 | 2213405 |
| anisakidae larvae found in marine fishes and squids from the gulf of tongking, the east china sea and the yellow sea. | marine fishes and squids caught in the gulf of tongking, the east china sea and the yellow sea were dissected, and many anisakidae larvae were found in them. the larvae of anisakis simplex (anisakis type i larvae), the most important causative agent of anisakiasis, was found in about 30% of fishes and squids caught in the gulf of tongking and in about 60% of those caught in the east china sea and the yellow sea. larvae belonging to three other genera than anisakis--raphidascaris or raphidascaroi ... | 1991 | 1784084 |
| [seroepidemiologic data on anisakiasis. prophylactic consequences for fish products]. | the western blotting method allowed us to confirm the diagnosis in 7 cases of acute anisakiasis, to assess it in 5 cases of chronic anisakiasis, and to follow up the evolution. these chronic frames according to the question of their long term evolution, and the part played by anisakis simplex in fish nutritional allergy increase the negative score of that parasite. this situation should lead to discuss preventive rules. | 1991 | 1772241 |
| biological tags for stock separation in pacific herring clupea harengus pallasi in california. | a survey of the parasites of pacific herring (clupea harengus pallasi) off northern california identified 1 species of acanthocephala, 1 species of cestoda, 2 species of copepoda, 1 species and 1 family of digenea, 3 species of nematoda, and 3 species of protozoa. from this survey, lacistorhynchus dollfusi (cestoda), parahemiurus merus (digenea), and anisakis simplex, contracaecum sp., hysterothylacium sp. (nematoda) were selected as potential tags. herring were collected in tomales, san francis ... | 1992 | 1738068 |
| anisakis simplex: histopathological changes in experimentally infected cba/j mice. | third-stage juveniles (l3) of anisakis simplex, surgically implanted into the abdominal cavity of cba/j mice and necropsied at 7, 14, or 21 days postinfection (pi), embedded in the gut mesentery and only rarely invaded viscera. histologically, intense aggregations of neutrophils adjacent to the parasites were noted at day 7 pi. at day 14 pi, mature granulomata consisting mostly of eosinophils and large numbers of fibroblasts and associated collagen were observed. granulocytes and occasionally mu ... | 1990 | 2311712 |
| a case of abdominal syndrome caused by the presence of a large number of anisakis larvae. | a 58-year-old woman living in shizuoka prefecture, japan, complained of severe epigastric pain and nausea about 8 h after having eaten 'sashimi'. a gastrocamera examination undertaken as a result of her clinical history of gastric anisakiasis indicated that a large number of worms had penetrated the posterior walls. fifty-six larval nematodes were directly removed from the greater curvature of the stomach with a gastroendoscopic biopsy clipper, and were identified as the larvae of anisakis simpl ... | 1992 | 1587691 |
| [morphological and taxonomical studies on anisakidae larvae found in marine fishes of china. ii. gulf of tong king]. | a survey on anisakidae larvae in 29 species (134 specimens) of marine fishes in the gulf of tong king has been carried out. anisakidae larvae were detected in 15 out of 29 species. the detected specimens were identified as larvae of anisakis simplex, hysterothylacium and pseudoterranova. the parasitization rate of anisakis simplex larvae, the main pathogen of anisakiasis, in fishes was 30.6% (41/134), while the parasitization rates of hysterothylacium and pseudoterranova larvae were comparativel ... | 1992 | 1394905 |
| radiolabelling of the excretory-secretory and somatic antigens of anisakis simplex larvae. | anisakis simplex larvae were cultured in vitro in medium containing 35s-methionine for ten days. the medium and the larval tissues were analysed for biosynthetically labelled polypeptide by sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography. immunoprecipitates with positive and negative human antisera were similarly analysed, using staphylococcus aureus to absorb immuno-complexes. es products of anisakis larvae contained many polypeptides with molecular weights of les ... | 1992 | 1293198 |
| [detection of antibodies against the larva of anisakis simplex in the pollock pollachius virens using elisa]. | from the fact that older saithes (more than 5 years old) are showing significantly lower prevalence of attack by anisakis larvae in lateral muscle than younger saithes (3-4 years old), the question arises if this phenomenon is based on a specific immune response. therefore we have investigated serum samples from saithes of different ages by using an indirect elisa to estimate the antibody-titer against excretory-secretory anisakis antigen. results showing a moderate correlation (r = 0.66) betwee ... | 1991 | 1858459 |
| [pseudotumor form of herring worm disease]. | | 1975 | 1220077 |
| anisakis simplex and anisakis physeteris: physicochemical properties of larval and adult hemoglobins. | | 1979 | 573208 |
| response of mink (mustela vison) to larval anisakis simplex (nematoda: ascaridida). | | 1977 | 562861 |
| [the herring worm disease (anisakiasis)]. | | 1979 | 452757 |
| [herring-worm disease in 1965]. | | 1966 | 5932968 |
| prevalence of larval anisakis simplex in pen-reared and wild-caught salmon (salmonidae) from puget sound, washington. | the abundance of parasites of public health significance in pen-reared salmon and wild-caught salmon was compared. two hundred eighty-seven salmon from puget sound, washington, were examined for third-stage larvae of anisakis simplex. of these fish, 237 atlantic salmon (salmo salar), coho salmon (oncorhynchus kisutch) and chinook salmon (o. tshawytscha) were reared in commercial salmon pens and 50 sockeye salmon (o. nerka) were caught during their spawning migration. all wild-caught salmon were ... | 1989 | 2761015 |
| anisakis simplex: uncoupling of oxidative phosphorylation in the muscle mitochondria of infected fish. | adenosine triphosphatase activity stimulated by mg2+ was greater in muscle mitochondria of fish infected with larval anisakis simplex nematodes than in uninfected fish. when muscle mitochondria were isolated in a sucrose ethylene-glycol bis(beta-aminoethyl ether)n,n'-tetraacetic acid medium from fresh uninfected fish, they were loosely coupled, and their adenosine triphosphatase activity was comparable to that of mitochondria from rat tissue. activity in infected fish was dose dependent, increas ... | 1986 | 2937649 |
| the secreted and somatic antigens of the third stage larva of anisakis simplex, and antigenic relationship with ascaris suum, ascaris lumbricoides, and toxocara canis. | the in vitro-released 'excretory/secretory' (es) and somatic antigens of the third stage (infective) larva of anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. oral infection with the parasite elicited a substantial igg antibody response to es in infected rabbits and humans, with a minimal response to somatic materials. serial serum sampling in experimental infection showed that there was a sequential reco ... | 1988 | 3054540 |
| [anisakids and human anisakiasis. 2. investigation of the anisakids of commercial fish in the district of paris]. | 1,173 fish belonging to 13 species were examined during 1986-1987, during a survey conducted among commercial marine fish in the paris area (france). anisakis simplex l3 were found in 10 different species, and most frequently in herring (clupea harengus) 82.55% of 682 fish, red fish (sebastes marinus) 86.11% of 36 fish, and hake (merluccius merluccius) 88.57% of 35 fish, and in high number (average 8.8, 18.4 and 31 l3 per fish). pseudoterranova decipiens l3 were recovered from 7 different specie ... | 1988 | 3190121 |
| nematode infestation of fillets from atlantic cod, gadus morhua, off eastern canada. | the prevalence and intensity of larval nematodes in fillets of atlantic cod, gadus morhua, were examined and compared with similar data from a previous survey conducted about 30 yr ago. anisakis simplex occurred more often in the nape of the fillet, whereas pseudoterranova decipiens was the predominant species in napeless fillets. the results suggest an increase in both the prevalence and intensity of p. decipiens in fillets of cod, especially those originating from the gulf of st. lawrence and ... | 1988 | 3193325 |
| [intestinal anisakiasis (herring worm disease). report of 8 acute disease courses]. | | 1988 | 3211862 |
| [ileus of the small intestine caused by intestinal anisakiasis (herring worm disease)]. | two cases of anisaciasis caused by eating of improperly prepared herrings which were infested with larval nematodes are reported. the acute type with a stenosing process in the small intestinal wall existed in both cases which resulted in an ileus. therapy was resection of the affected intestinal part with end-to-end-anastomosis. the morphological proof of larval nematodes, which penetrate from lumen into the eosinophilic granulomata, pseudotumours and eosinophilic microabscesses are important h ... | 1988 | 3215066 |
| [occurrence of parasites, bacteria, viruses and fungi in fish that are pathogenic to men and fish]. | parasitological examinations comprised above 20,000 fish which were searched for parasitic nematoda of anisakidae. it was evidenced that herring were infected with anisakid larvae in 8%, cod and flatfish in 4% and the eelpouts in as many as in 52%. the species that prevails in these fish were: anisakis simplex, contracaecum osculatum c, hysterothylacium auctum and pseudoterranova decipiens, respectively. in direction of the bacteria pathogenic to man 765 fish were examined; in 38% there were fou ... | 1995 | 7571631 |
| anisakiasis caused by herring in vinegar: a little known medical problem. | six cases of intestinal anisakiasis, or herring worm disease, diagnosed over a two year period in a belgian gastroenterology unit are described. they presented mainly as intestinal obstructions and larvae of this marine nematode were found in the intestinal wall of two of the four patients who were operated on. in two other patients awareness of the diagnosis permitted conservative treatment and spontaneous healing. in five patients symptoms developed after they had eaten herring marinated in vi ... | 1988 | 3384369 |
| commercial blast-freezing of third-stage anisakis simplex larvae encapsulated in salmon and rockfish. | sixty-four fish were blast-frozen to -35 c for 15 hr to determine the effects of commercial blast-freezing on the viability of third-stage larvae of anisakis simplex encapsulated in the muscle and viscera of sockeye salmon (oncorhynchus nerka) and canary rockfish (sebastes pinniger). parallel tests were conducted on larval nematodes in 16 whole (round) salmon, 16 dressed salmon (heads and viscera removed), and 32 whole (round) rockfish. after blast-freezing, 4 in-the-round salmon, 4 dressed salm ... | 1988 | 3397820 |
| a simple technique for recovering larval ascaridoid nematodes from the flesh of marine fish. | this paper describes and evaluates the efficiency of a simple technique for recovering larval ascaridoid nematodes (anisakis simplex and pseudoterranova decipiens) from the flesh of marine fish. the technique involves mechanical disintegration of the flesh in a domestic food processor, followed by visual inspection of diluted portions of the resulting homogenate under short-wave ultraviolet light. the nematodes, which remain intact, fluorescence brightly and are easily detected, particularly if ... | 1988 | 3397837 |
| anisakis simplex: larval excretory secretory protein production and cytostatic action in mammalian cell cultures. | excretory secretory proteins produced in vitro by anisakis simplex larvae incubated in medium 199 or phosphate buffered saline with dextrose are similar with respect to protein content and biological activity. analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis suggested that the molecular weight of the component(s) responsible for inhibition of mitogen induced lymphocyte blastogenesis is between 66,000 and 95,000. in vitro production of excretory secretory protein, approximate ... | 1986 | 3720903 |
| genetic structure of anisakis physeteris, and its differentiation from the anisakis simplex complex (ascaridida: anisakidae). | the genetic structure of anisakis physeteris from the mediterranean sea has been analysed electrophoretically at 22 enzyme loci. the samples studied, although differing in the life-stage (larvae and adults), and in the host (the fishes micromesistius poutassou and trachurus trachurus, and the sperm whale physeter macrocephalus) were genetically homogeneous. of these loci 11 (ldh, sod, np, adk-2, pgm-1, est-1, est-2, acph-1, acph-2, lap-2 and ca) were found to be monomorphic, while the other 11 ( ... | 1986 | 3785976 |
| electrophoretic studies on the anisakis simplex complex (ascaridida:anisakidae) from the mediterranean and north-east atlantic. | | 1986 | 3804573 |
| invasive anisakiasis. a case report from hawaii. | a confirmed case of penetration into the stomach wall by an anisakine nematode, to our knowledge the first in the united states, is reported. a viable third-stage larva of anisakis simplex was removed from a woman in hawaii who had eaten fresh, raw salmon. a serodiagnostic test showed the serum of the patient to be positive for a. simplex-specific ige at days 11 and 60 postinfection. our results indicate that eating fresh, raw salmon exposes consumers to a significant health risk. | 1986 | 3949105 |
| hyaluronidases of the gastrointestinal invasive nematodes ancylostoma caninum and anisakis simplex: possible functions in the pathogenesis of human zoonoses. | the gastrointestinal invasive stages of two parasitic nematodes, ancylostoma caninum and anisakis simplex, were each found to release in vitro a hydrolytic enzyme that degrades the glycosaminoglycan hyaluronic acid. the parasite hyaluronidases were partially purified by ion-exchange chromatography and biochemically characterized. the hyaluronidase from a. caninum adult worms had a molecular weight of 65,000 and a ph optimum of 6 with activity at neutral ph, while the hyaluronidase from a. simple ... | 1994 | 7930737 |
| eosinophilic colitis. | eosinophilic gastroenteritis, a condition of unknown etiology, has frequently been reported involving the stomach and small bowel. the colon is rarely cited as a site for the condition; however, a review of the literature reveals over 20 cases (including ours). the findings indicate that the colon is now a recognized, but rare, site of involvement and that the condition can be diagnosed via the colonoscope. no clear-cut etiology can be defined, but the presence of allergy (33 percent) and of the ... | 1985 | 4017826 |
| [from anisakis simplex invasiology (author's transl)]. | | 1974 | 4445747 |
| [studies on the survival of nematode anisakis simplex larvae (author's transl)]. | | 1974 | 4475477 |
| gastrointestinal helminths of the cetaceans phocoena spinipinnis (burmeister, 1865) and cephalorhynchus eutropia (gray, 1846) from the southern coast of chile. | gastrointestinal helminths of the cetaceans cephalorhynchus eutropia and phocoena spinipinnis accidentally entangled in gillnets off the coast of queule, chile, were identified from 1989 to 1990. pseudoterranova sp., polymorphus (polymorphus) cetaceum and synthesium tursionis occurred in both cetaceans. additionally, anisakis sp. and braunina cordiformis were found in c. eutropia, and anisakis simplex was identified from p. spinipinnis. the species with the highest prevalence and mean intensity ... | 1992 | 1602588 |
| [developmental cycle of anisakis simplex (rudolphi, 1809) in the light of recent studies]. | | 1973 | 4714754 |
| [a case of herring worm disease]. | | 1969 | 5352503 |
| [herring worm disease (anisakiasis)]. | | 1970 | 5446182 |
| [herring worm disease (anisakiasis)]. | | 1968 | 5691355 |
| [herring worm disease (anisakiasis)]. | | 1969 | 5768665 |
| [the herring worm anisakis marina as a human parasite outside the wall of the gastrointestinal tract]. | | 1966 | 5914948 |
| [herring worm disease (anisakiasis)]. | | 1967 | 6044066 |
| molecular cloning of the cdna encoding a 42 kda antigenic polypeptide of anisakis simplex larvae. | the gene encoding an antigenic polypeptide of anisakis simplex larvae was studied using recombinant dna techniques. cdna synthesized from poly(a)-rich mrna from a. simplex larvae was ligated into phage vector lambda gtll dna and packaged in vitro. the phages were propagated on escherichia coli and a lambda gtll expression library was constructed. a cdna clone encoding a 42 kda antigenic polypeptide was selected by immunoscreening of the library and identified by the epitope selection method. a c ... | 1992 | 1281853 |
| changes of igm and igg antibody levels in experimental rabbit anisakiasis as observed by elisa and sds-page/immunoblot. | antibody changes in experimental anisakiasis were observed in 10 rabbits which were infected each with 10 anisakis simplex larvae. the sera were collected before and on the 6th to the 95th day after the infection. using crude saline extract of anisakis larvae as antigen, specific igm and igg antibody levels were observed by elisa and sds-polyacrylamide gel electrophoresis/immunoblot. levels of specific-igm antibody were elevated from the 6th day, reached their peaks on the 11th day after the inf ... | 1991 | 1804303 |
| anisakis simplex (rudolphi, 1809, det. krabbe, 1878) (nematoda: ascaridoidea): morphology and morphometry of larvae from euphausiids and fish, and a review of the life-history and ecology. | larval anisakis 7.7 to 23.6 mm long from euphausiids, and larvae 18.0 to 21.9 mm long from a teleost fish were morphologically and morphometrically alike and fitted the description by beverley-burton, nyman & pippy (1977) of a. simplex l3. three small larvae, 4.2 to 5.9 mm long, from euphasiids resembled l3 in anterior but not in posterior morphology; their tails lacked a mucron but, in two of them, cuticular separation at the posterior extremity revealed clearly the mucron characteristic of l3 ... | 1983 | 6685155 |
| anisakis simplex and terranova sp.: inhibition by larval excretory-secretory products of mitogen-induced rodent lymphoblast proliferation. | excretory-secretory products were collected from supernatants of in vitro cultures of larval nematodes, anisakis simplex (type i) and terranova sp. (hawaii type a). these materials were found to be more potent inhibitors of rodent lymphocyte blast transformation induced by concanavalin a and bacterial lipopolysaccaride than whole worm extracts of the same parasites. inhibition of blast transformation was a result of cytostatic rather than toxic effects on proliferating lymphoid cells. the materi ... | 1983 | 6852167 |
| [gastric anisakiasis cases in cheju-do, korea]. | human anisakiasis may occur after ingestion of raw marine fish infected with nematode larvae of anisakidae. anisakiasis caused by the migration of the larva into the wall of stomach, small intestine and other portion has been reported in korea. this prospective study was made of all cases referred to parasitological laboratory in cheju-do between june 1989 and june 1992. gastric anisakiasis was confirmed if larvae invading the gastric wall were observed by gastrofiberscopy. one hundred and seven ... | 1995 | 8528624 |
| specific antibody patterns of wistar rats inoculated with third stage larvae of anisakis simplex. | levels of excretory-secretory (es)-specific antibody were measured in wistar rats inoculated with 1, 5, or 20 third stage larvae (l3) of anisakis simplex. primary inoculation induced es-specific igm and igg titers, paralleling inoculum size. secondary inoculations resulted in an igm and igg titer augmentation in 1 or 5 larvae-inoculated animals to levels comparable to those of animals inoculated with 20 larvae, but titers in high inoculum animals did not increase upon secondary inoculation. prim ... | 1995 | 7623194 |
| [contribution to the morphology and pathogenesis of eosinophilic colitis (author's transl)]. | a 40 year old woman suffered from acute diarrhea and convulsive abdominal pains. because of a palpable tumor of the right lower abdomen, which was thought to be localized in the deeper layers of the colon ascendens, a hemicolectomy on the right side was performed. histologically there was a severe phlegmonous eosinophilic inflammation of the bowel wall and the surrounding fatty tissue. a parasite was found within the strongly oedematous submucosal layer. the different forms of eosinophilic infla ... | 1981 | 7303781 |
| recurrent anaphylaxis caused by anisakis simplex parasitizing fish. | | 1995 | 7560669 |
| [morphological changes of anisakiasis]. | we describe 5 own cases of anisakiasis, sometimes called "herring worm disease", which, in our patients, was acquired by the consumption of raw or undercooked herring containing the larvae of the anisakis nematodes. these cases are apparently the first observed in gdr. clinically, all patients presented acute abdominal syndromes. on account of stenosing eosinophilic intestinal wall phlegmons associated with focal or diffuse peritonitis therapy included resection of 9 to 20 cm long segments of th ... | 1982 | 6891546 |
| [anisakis simplex (larva iii stage) in stickleback gasterosteus aculeatus from the southern baltic region]. | one specimen of iii larval stage of anisakis simplex was found in body cavity of female stickleback gasterosteus aculeatus caught in southern baltic, in koĊobrzeg. in stickleback from baltic sea a. simplex was noted for the first time. it usually occurs in many other species of fish. | 1994 | 7801645 |
| detection of antibodies to anisakis simplex larvae by enzyme-linked immunosorbent assay and immunoelectrophoresis using crude or purified antigens. | the enzyme-linked immunosorbent assay (elisa) and immunoelectrophoresis (iep) were used for the serodiagnosis of larval anisakis simplex infections in man and immunized rabbits. sephacryl s-300 gel filtration was used for separating crude antigen. four fractions were obtained. sera from patients with other helminth infections sometimes cross reacted with anisakis larval antigens. with iep, crude antigen is more sensitive than purified antigens. with elisa, the third fraction is the most sensitiv ... | 1994 | 7706676 |
| anisakiasis ('herring worm disease') as a cause of acute abdominal crisis. | a hazard associated with eating raw fish is presented. the larval nematode anisakis marina ('herring worm') is a recognised public health problem in japan, and cases have been reported in the uk. the intestinal burrowing of the larval form causes acute abdominal symptoms clinically resembling acute appendicitis. operation is required; no antiparasitic agent is available. | 1994 | 7917804 |
| characterization of the serine protease and serine protease inhibitor from the tissue-penetrating nematode anisakis simplex. | a serine protease and a serine protease inhibitor were purified from infective larvae of the parasitic nematode anisakis simplex. the serine protease was found to be trypsin-like and preferentially cleaved substrates with the basic amino acid arginine at the p1 position (z-gly-pro-arg-amc (where z is benzyloxycarbonyl), km = 0.019 mm, and z-phe-pro-arg-amc, km = 0.013 mm) at rates similar to those determined for trypsin (0.002 mm and 0.006 mm, respectively). however, the presence of a bulky hydr ... | 1994 | 7961683 |
| [the problem of anisakiasis]. | presents a brief analysis of the basic findings concerning the pathogenesis, clinical picture, diagnosis, prevention, and epidemiology of anisakiasis in the world. suggests an opinion on a high prevalence of anisakiasis in some regions of russia, where it is erroneously diagnosed as some other gastrointestinal diseases. presents the author's data on the infestation of fish with anisakis simplex and pseudoterranova decipiens larvae in the okhotsk and bering seas. emphasizes the necessity of devel ... | 1993 | 8028570 |
| the radioallergosorbent test (rast) for the serological diagnosis of human anisakiasis. | the serum of patients with parasitologically confirmed and one patient with a clinically presumptive case of anisakiasis were tested by the radioallergosorbent test (rast) and counter-electrophoresis (cep) for ige and igg antibodies, respectively, using antigens prepared from larval (l3) stage anisakis simplex and larval (l2) stage toxocara canis. all sera were rast-positive to the a. simplex antigen and rast-negative to the t. canis antigen. all sera were cep-negative to both antigens. the pres ... | 1985 | 4039859 |
| intestinal parasites of cats purchased in seoul. | fecal samples of cats purchased in seoul were examined for helminth ova or protozoan oocysts from december 1987 to march 1988. out of the 41 samples, 31 (75.6%) were positive and 60 (146.3%) were cumulative positive for parasites. the followings were identified in the samples: eggs of toxocara cati, clonorchis sinensis, metagonimus sp., pharyngostomum cordatum, spirometra erinacei, taenia taeniaeformis and oocysts of isospora sp. from nine autopsied cats, larvae of anisakis simplex, adults of c. ... | 1993 | 8297895 |
| anisakis simplex: antigen recognition and antibody production in experimentally infected mice. | the kinetics of antibody response to intraperitoneal infection of mice with third stage larvae of anisakis simplex was investigated by elisa. maximum antibody response to excretion-secretion (es) antigens was reached before maximum response to somatic (sa) antigens. total immunoglobulin (ig) production (consisting mainly of igm and igg1 isotypes) was very similar in both cases. immunoblotting was used to characterize the antigens recognized by the host in the presence or absence of the metabolic ... | 1993 | 7687339 |
| alterations in polyamine levels of nematode, earthworm, leech and planarian during regeneration, temperature and osmotic stresses. | free-living nematodes, caenorhabditis elegans and dorylaimus fodori, contain putrescine and spermidine. putrescine, spermidine and spermine occur in the parasitic nematoda, ascaris suum, anisakis simplex and dirofilaria immitis. earthworms, eisenia foetida, tubifex hattai and pheretima communissima and the leech, hirudo nipponia (belonging to annelida) and the planarian, dugesia japonica (belonging to platyhelminthes) contain homospermidine and spermine in addition to putrescine and spermidine. ... | 1995 | 7749639 |
| larval anisakid nematodes of japanese common squid (todarodes pacificus) from the sea of japan. | two species of third-stage larvae of the family anisakidae are described from the japanese common squid (todarodes pacificus steenstrup) from the sea of japan. the larvae of 1 of them, anisakis simplex (rudolphi, 1809), commonly occur encapsulated in the viscera of squid, whereas those of another 1, identified as lappetascaris sp., are found free in the mantle musculature. the latter, characterized by the presence of a very long ventricular appendix, a conspicuous esophageal gland, and the struc ... | 1995 | 7876981 |
| anisakis simplex: stage-specific antigens recognized by mice. | to investigate antigenic differences between the developmental stages of anisakis simplex, somatic, excretion-secretion and detergent-solubilized surface antigen preparations of third- and fourth-stage a. simplex larvae, and a somatic antigen preparation of adults, were characterized by sds-page and immunoblotting using mouse immune serum containing antibodies against the two larval stages. excretion-secretion and surface antigen preparations from third-stage larvae behaved very differently from ... | 1995 | 8583126 |
| [stenosis of the small intestine as a late sequela of a herring worm phlegmon]. | | 1967 | 6044048 |
| laboratory determinations in anisakis simplex allergy. | anaphylactic reactions caused by the fish nematode, anisakis simplex, after ingestion of parasitized fish, have been described. | 1996 | 8655894 |
| larval anisakids (nematoda:ascaridoidea) in horse mackerel (trachurus trachurus) from the fish market in granada, spain. | horse mackerel (trachurus trachurus) from the fish market in granada, southern spain, were surveyed for anisakid nematodes. the fish came from fishing ports all over the country. larval anisakids were found in 39.4% of the fish examined; 26.1% were infected with third-stage larvae (l3s) of anisakis simplex; 0.3%, with a. physeteris l3s; 31.1%, with hysterothylacium aduncum l3s; and 1.7%, with fourth-stage larvae (l4s) of hysterothylacium sp. horse mackerel from mediterranean sea coastal ports (s ... | 1996 | 8740547 |
| antigenic cross-reactivity in mice between third-stage larvae of anisakis simplex and other nematodes. | we used elisa and immunoblotting to investigate antigenic cross-reactivity in mice between third-stage larvae of anisakis simplex and five other nematodes: the ascaridoids ascaris suum, toxocara canis and hysterothylacium aduncum, and the nonascaridoids trichinella spiralis and trichuris muris. two sera were raised against each species (including a. simplex, but excluding a. suum), by infection or by immunization with somatic antigens. serum against a. suum was raised by immunization only. the r ... | 1996 | 8740558 |
| cooking and freezing may not protect against allergenic reactions to ingested anisakis simplex antigens in humans. | | 1997 | 9076923 |
| free and bound biotin molecules in helminths: a source of artifacts for avidin biotin-based immunoassays. | the avidin-biotin molecular recognition system is widely used in parasite immunology. however, the presence of biotin and/or biotin-containing molecules (bcms) in samples may lead to erroneous results. in the work reported herein we investigated the extent to which biotin and bcms present in helminth extracts may interfere in avidin/biotin-based immunoassays and developed an enzyme-linked immunosorbent assay (elisa) for quantification of these components. in avidin-based elisa using antinematode ... | 1996 | 8875569 |
| anisakis simplex induces not only anisakiasis: report on 28 cases of allergy caused by this nematode. | anisakis simplex r., parasitizes several sea-fish and cephalopods. this parasite induces not only anisakiasis but also anaphylactic reactions, as has recently been confirmed. we present 28 patients with immediate hypersensitivity to anisakis simplex, developed after parasitized fish ingestion. each case was diagnosed by suggestive anamnesis, skin prick tests with an a. simplex extract, specific ige detection in serum (cap system), and histamine release test. the clinical manifestations were urti ... | 1996 | 8959543 |
| leukocyte parameters in the course of pig infection with iii larval stage of anisakis simplex. | experimental anisakiosis covered 6 pigs infected with iii larval stage of a. simplex. changes in leukocytes were analysed. the blood showed an increased number of eosinophiles after 5th day. the number of high-affinity and eac rosettes decreased after 8th day. | 1996 | 8909179 |
| rheumatic manifestations in the course of anaphylaxis caused by anisakis simplex. | human anisakidosis is a parasitic infection caused by the larvae of anisakis simplex. classical clinical manifestations include epigastric pain, occlusion, diffuse abdominal pain, appendicitis, and anaphylactoid reactions. arthralgias or arthritis have been infrequently reported. we present three patients with proven hypersensitivity to a. simplex together with rheumatologic complaints after ingestion of parasitized fishes. a. simplex must be considered in the differential diagnosis of arthralgi ... | 1998 | 9631754 |
| the use of ige immunoblotting as a diagnostic tool in anisakis simplex allergy. | the fish parasite anisakis simplex is the etiologic agent of anisakiasis and induces ige-mediated reactions. skin prick tests (spts) and the measurement of specific ige to a. simplex were, in our experience, not valid tools with which to discriminate between allergic and nonallergic patients because many control subjects also had positive results. | 1997 | 9111494 |
| the infection status of anisakid larvae in marine fish and cephalopods from the bohai sea, china and their taxonomical consideration. | the infection status of anisakid larvae was examined in 290 marine fish of 25 species and in 108 cephalopods of 3 species purchased in bayuquan region, yingko city nearby the coast of the bohai sea from may to august 1992. a total of 7,327 larvae were collected from 156 fish of 19 species and 8 squids of one species. the 3rd-stage larvae of anisakis simplex were collected from 121 fish (63.4%) of 15 species (n = 191) and from 8 squids (14.8%) of one species (n = 54), and they were total, 5,992 ( ... | 1997 | 9100435 |
| in vitro study on the effect of larval excretory/secretory products and crude extracts from anisakis simplex on blood coagulation. | the anticoagulant action of anisakis simplex larvae on human blood in vitro was examined. anticoagulant activity was assessed by routine screening tests that evaluate the overall competency of the coagulant mechanism. a slight prolongation of the prothrombin time (pt) was observed with the larval crude extracts. prolongation of the pt was seen at a concentration of excretory/secretory (es) products greater than 62.5 micrograms/ml. no prolongation of the activated partial thromboplastin time (ptt ... | 1996 | 9198584 |
| sensitization to anisakis simplex: an unusual presentation. | it has recently been published patients suffering from urticaria or anaphylaxia induced by nematodes usually parasitizing fishs or cephalopode, in whom, anisakis simplex (ak) sensitization prevalence was detected up to 37%. we tried out a prospective study to evaluate the presence of ak specific-ige in an asthmatic population, comparing to other group of patients with urticaria. complaints related to food ingestion were recorded in both, and dietetic measures were advised. thirteen patients (13/ ... | 1997 | 9150840 |
| anisakis simplex, a relevant etiologic factor in acute urticaria. | anisakis simplex, a parasite of fish and cephalopods, can induce ige-mediated reactions. this study aimed to determine the etiologic role of a. simplex in patients affected by urticaria/angioedema (ae) or anaphylaxis. we studied 100 adult subjects suffering acute episodes of urticaria/ae, by anamnesis, prick tests with a. simplex and fish-mix extracts, and total and specific ige to both a. simplex and cod. the following criteria of a. simplex allergy were considered: 1) urticaria/ae within 6 h a ... | 1997 | 9201371 |
| anaphylaxis with anisakis simplex in the gastric mucosa. | | 1997 | 9235506 |
| genetic and ecological data on the anisakis simplex complex, with evidence for a new species (nematoda, ascaridoidea, anisakidae). | isozyme analysis at 24 loci was carried out on anisakid nematodes of the anisakis simplex complex, recovered from various intermediate/paratenic (squid, fish) and definitive (marine mammals) hosts from various parts of the world. a number of samples were found to belong to a. simplex sensu stricto and anisakis pegreffii, widely extending the geographic ranges and the number of hosts of these 2 species. in addition, a new distinct gene pool was detected, showing different alleles with respect to ... | 1997 | 9194819 |
| periodate treatment of anisakis simplex allergens. | anaphylactic reactions after parasitized fish consumption are mediated by an ige response. however, positive skin tests and specific ige can also be found in many asymptomatic subjects who recognize a single medium-mol.-wt. antigen by ige immunoblot. the study aimed to find out whether this unspecificity was due to the carbohydrate moieties of parasite antigens. sixty-two patients with suspected parasite allergy, 51 blood donors, 18 bakers, and 38 atopic patients were studied by blotting. parasi ... | 1997 | 9201369 |
| cross-reactivity between ige-binding proteins from anisakis, german cockroach, and chironomids. | anisakis simplex larvae parasitize animals used as seafood and can produce a specific immune response in man. the ingestion of seafood contaminated with stage three of a. simplex larvae can induce a specific ige response with clinical symptoms, usually urticaria, even if the fish is cooked before ingestion and the invasive infestation power destroyed by heating. our preliminary studies showed a strong association of a. simplex sensitization with ascaris lumbricoides, daphnia, chironomid spp., at ... | 1997 | 9201362 |
| recidivous acute urticaria caused by anisakis simplex. | this study aimed to determine the cause of acute recidivous urticaria in patients who usually eat fish or other seafood. twenty-five patients were studied. the skin prick test with larval anisakis simplex extract was performed; total and specific ige against a. simplex was measured with the cap system; specific antibodies to a. simplex were determined by elisa; and immunorecognition patterns of the sera were studied by western blot. nineteen patients showed specific ige to a. simplex, but specif ... | 1997 | 9360749 |
| isotype-specific immune responses in murine experimental anisakiasis. | a murine experimental model of anisakiasis has been developed in balb/c and c57bl/10 mice orally inoculated with an anisakis simplex living third stage larva (l3) in order to investigate isotype-specific immune responses against excretory-secretory (es) products and crude extracts (ce) from l3. specific antibody production showed similar patterns against both es and ce antigens with higher levels against the latter. the dynamics of the production showed the earliest responses in balb/c, in which ... | 1998 | 9916551 |
| [anisakiasis in the nantes area. from fishmongers' stalls to medical offices]. | 1,103 fish-fillets belonging to 4 species were examined during one year, during a survey conducted among commercial marine fish-fillets in the nantes area (west coast) in france. anisakis simplex l3 were found in 3 different species and most of them are frequently present in coal fish (pollachius virens) and whiting (merlangius merlangus). we report 6 cases of human anisakiasis (3 certain cases and 3 probably cases). diagnosis was established in 3 cases by microscopic analysis of intestin bowel ... | 1995 | 8745739 |
| monoclonal antibodies against diagnostic anisakis simplex antigens. | five monoclonal antibodies (ua2, ua3, ua5, ua6, and ua8) specific for anisakis simplex are described. all are igg1/kappa monoclonal antibodies, except for ua2, which is an antibody igm/kappa. the molecular weights of the major components recognized in immunoblotting are 48 and 67 kda (ua2); 139 kda (ua3 and ua5; same epitope); 35, 38, and 139 kda (ua6); and 205 kda (ua8). ua2 was the only monoclonal antibody to recognize both components of an excretion-secretion antigen preparation and antigens ... | 1997 | 9342739 |
| [anisakidosis of the colon as a cause of acute abdomen]. | we report a case of acute abdomen caused by the invasion of the ascending colon by larvae of anisakis simplex in a 57 year-old-woman whose initial symptoms were colic abdominal pain, diarrhea and fever. the diagnosis was done by the histological study of the surgical specimen in which two parasites were morphologically identified in the submucosa. although a causal relationship of the disease with the ingestion of crude fish could not be established initially, there were antecedents of ingestion ... | 1997 | 9280431 |
| parasites of fish and risks to public health. | a multitude of parasites have been reported in fish, but only a few species are capable of infecting humans. the most important of the helminths acquired by humans from fish are the anisakid nematodes (particularly anisakis simplex and pseudoterranova decipiens), cestodes of the genus diphyllobothrium and digenetic trematodes of the families heterophyidae, opisthorchiidae and nanophyetidae. seafood-associated infections by acanthocephalans are rarely reported in humans. all of the helminths ment ... | 1997 | 9501379 |
| allergic reactions to anisakis simplex parasitizing seafood. | the ingestion of anisakidae ssp larvae parasitized fish can cause anisakiasis. allergic reactions after ingestion of safely cooked but parasitized fish have been reported. | 1997 | 9305232 |
| evidence for arylamine n-acetyltransferase in the nematode anisakis simplex. | n-acetyltransferase activities with p-aminobenzoic acid and 2-aminofluorene were determined in anisakis simplex, a nematode found in the intestine of the salt water fish trichiurus lepturus. the n-acetyltransferase activity was determined using an acetyl coa recycling assay and high pressure liquid chromatography. the n-acetyltransferase activity from a number of anisakis simplex whole tissue homogenizations was found to be 2.89 +/- 0.52 nmol/min per mg for 2-aminofluorene and 2.54 +/- 0.45 nmol ... | 1996 | 8827040 |
| distribution of whaleworm (anisakis simplex, nematoda, ascaridoidea) l3 larvae in three species of marine fish; saithe (pollachius virens (l.)), cod (gadus morhua l.) and redfish (sebastes marinus (l.)) from norwegian waters. | the frequency distribution of anisakis simplex l3 larvae between host tissues was investigated in three host species: saithe, cod and redfish. fish were sampled from norwegian coastal waters and examined for the presence of a. simplex in muscle and viscera. in all three of the host species, a. simplex larvae were most frequently detected in the viscera; the percentages of total infection for saithe, cod and redfish were 99.6%, 97.8% and 88.0%, respectively. in general, the distribution patterns ... | 1998 | 9569092 |
| allergenic cross-reactivity between third stage larvae of hysterothylacium aduncum and anisakis simplex. | | 1998 | 9580257 |