Publications

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toxicity of bacillus thuringiensis (l.) cry proteins against summer fruit tortrix (adoxophyes orana - fischer von rösslerstamm).the activity of seven cry1, one cry9 and one hybrid cry1 protoxins against neonate larvae of summer fruit tortrix (adoxophyes orana - fischer von rösslerstamm) has been investigated. cry1ia is identified as the most toxic protein, followed by cry1aa and cry1ac. cry1ca, cry1cb, cry1da and cry1fa were less active, while sn19 (cry1 hybrid protein with domain composition 1ba/1ia/1ba) and cry9aa exhibited negligible toxicity against a. orana. in vitro trypsin-activated cry1ac is still less active tha ...201627311897
genome sequencing and analysis of a granulovirus isolated from the asiatic rice leafroller, cnaphalocrocis medinalis.the complete genome of cnaphalocrocis medinalis granulovirus (cnmegv) from a serious migratory rice pest, cnaphalocrocis medinalis (lepidoptera: pyralidae), was sequenced using the roche 454 genome sequencer flx system (gs flx) with shotgun strategy and assembled by roche gs de novo assembler software. its circular double-stranded genome is 111,246 bp in size with a high a+t content of 64.8% and codes for 118 putative open reading frames (orfs). it contains 37 conserved baculovirus core orfs, 13 ...201526712716
isolation of an adoxophyes orana granulovirus (adorgv) occlusion body morphology mutant: biological activity, genome sequence and relationship to other isolates of adorgv.a granulovirus (gv) producing occlusion bodies (obs) with an unusual appearance was isolated from adoxophyes spp. larvae in the field. ultrastructural observations revealed that its obs were significantly larger and cuboidal in shape, rather than the standard ovo-cylindrical shape typical of gvs. n-terminal amino acid sequence analysis of the ob matrix protein from this virus suggested that this new isolate was a variant of adoxophyes orana granulovirus (adorgv). bioassays of this gv (termed ado ...201525524166
mating disruption of spilonota ocellana and other apple orchard tortricids using a multispecies reservoir dispenser.a new mating disruption formulation for population control of a wide range of tortricid pests, including spilonota ocellana, was tested in swedish apple orchards during 2012-2013. owing to the characteristics of the local agricultural landscape, mating disruption was evaluated in isolated orchards rather than through an area-wide approach. parameters such as trap shutdown, communication disruption in field cages, damage level and dispenser emission were measured as efficacy indicators.201524916099
complete genome sequence of agrotis segetum granulovirus shanghai strain.the complete nucleotide sequence of agrotis segetum granulovirus shanghai strain (agsegv-l1) was determined and compared with that of agsegv xinjiang strain (agsegv-xj). the circular genome of agsegv-l1 is 131,442 bp and has a g+c content of 37.27 %. it includes 149 orfs, 24 of which are unique to agsegv (agsegv-l1 and agsegv-xj [genbank accession no. nc_005839]). the average level of amino acid sequence identity between agsegv-l1 and other granulovirus (gv) homologues (except agsegv-xj) ranged ...201424519461
[influence of relative humidity on the adoxophyes orana experimental population].in order to explore the effects of relative humidity (rh) on the population growth of summer fruit tortrix moth adoxophyes orana, the development, survival, fecundity and longevity of a. orana were assessed at 6 different constant humidities (40%, 50%, 60%, 70%, 80% and 90%), (25 +/- 1) degrees c and a photoperiod of 12l:12d in the laboratory. the results showed that immature developmental time was gradually shortened with the increasing humidity at 40%-90% rh, ranged from 30.31 d at 40% rh to 2 ...201425876412
the complete mitochondrial genome of the summer fruit tortrix moth adoxophyes orana (lepidoptera: tortricidae).the complete mitochondrial genome of the summer fruit tortrix moth adoxophyes orana (lepdioptera: tortricidae) was determined (genbank accession no. jx872403). the genome is 15,343 bp long with 37 typical animal mitochondrial genes and an a+t-rich region. as in other sequenced mitochondrial genomes of lepidoptera, trnm was rearranged to the upstream of trni-trnq-trnm cluster compared with the pupative ancestral arrangement of insects. all protein-coding genes start with atn start codon except fo ...201323245567
fitness-related traits of entomopoxviruses isolated from adoxophyes honmai (lepidoptera: tortricidae) at three localities in japan.three entomopoxviruses (epvs) isolated from diseased adoxophyes honmai larvae at different localities (tsukuba, itsukaichi, and miyazaki) in japan were compared for biochemical identity and key parameters of virus fitness, fatal infection, speed of kill, and virus yield. when the structural peptides of occlusion bodies (obs) and occlusion-derived viral particles were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, no difference in banding patterns was observed. however, ...201020447402
sex pheromones and reproductive isolation of three species in genus adoxophyes.we tested differences in female pheromone production and male response in three species of the genus adoxophyes in korea. females of all three species produced mixtures of (z)-9-tetradecenyl acetate (z9-14:oac) and (z)-11-tetradecenyl acetate (z11-14:oac) as major components but in quite different ratios. the ratio of z9-14:oac and z11-14:oac in pheromone gland extracts was estimated to be ca. 100:200 for adoxophyes honmai, 100:25 for adoxophyes orana, and 100:4,000 for adoxophyes sp. field test ...200919221842
the origins of replication of granuloviruses.the genomes of eight granuloviruses (gvs), have been analyzed for the presence of homologous regions (hrs) that may act as origins of replication. thirteen 74-76-bp palindromes within 11 hrs have previously been identified in the cydia pomonella gv (cpgv) genome and found to replicate in an infection-dependent dna replication assay. we report a further palindrome within one of the hrs, which was found to replicate, bringing the total to 14 palindromes. we also report imperfect palindromes, with ...200818612587
biological characterization of an english granulovirus from the summer fruit tortrix moth, adoxophyes orana.adoxophyes orana granulovirus (adorgv) was isolated from overwintering larvae in an orchard in kent, in the uk. the developmental time of each a. orana instar was determined by measuring the size of the head capsule. the susceptibility of the larvae to the english isolate of adorgv was evaluated in laboratory bioassays using inoculation by microdroplet feeding and applied dose assays. a series of bioassays were performed to determine ld(50) and st(50) values for first, fourth and fifth instar la ...200817967464
genomic sequence and biological characterization of a nucleopolyhedrovirus isolated from the summer fruit tortrix, adoxophyes orana.adoxophyes orana nucleopolyhedrovirus (adornpv) was isolated from overwintering larvae from an orchard in the uk. the nucleotide sequence of the adornpv dna genome was determined and analysed. the genome contains 111724 bp and has a g+c content of 35.0 mol%. the analysis predicted 121 orfs of 150 nt or larger. of these putative genes, 118 were homologous to genes identified previously in the adoxophyes honmai nucleopolyhedrovirus (adhonpv) genome (83.3-100 % aa identity), and three adornpv orfs ...200818931089
detection of adoxophyes orana granulovirus in adoxophyes orana by pcr. 200818999895
identification, characterization and phylogenic analysis of conserved genes within the odvp-6e/odv-e56 gene region of choristoneura fumiferana granulovirus.the genes that are located within the odvp-6e/odv-e56 region of the choristoneura fumiferana granulovirus (chfugv) were identified by sequencing the 11 kb bamhi restriction fragment on the chfugv genome. the global gc content that was calculated from the data obtained from this genomic region was 34.96%. the open-reading frames (orfs), located within the odvp-6e/odv-e56 region, are presented and compared to the equivalent orfs that are located at the same region in other gvs. this region is comp ...200415469697
identification, characterization and phylogenic analysis of conserved genes within the p74 gene region of choristoneura fumiferana granulovirus genome.the genes located within the p74 gene region of the choristoneura fumiferana granulovirus (chfugv) were identified by sequencing an 8.9 kb bamhi restriction fragment on the chfugv genome. the global guanine-cytosine (gc) content of this region of the genome was 33.02%. this paper presents the orfs within the p74 gene region along with their transcriptional orientations. this region contains a total of 15 open reading frames (orfs). among those, 8 orfs were found to be homologues to the baculovir ...200415607029
methoxyfenozide, a reliable ipm compatible compound against lepidoptera in pome fruit and vegetables with sterilising, ovicidal and larvicidal efficacy on codling moth.methoxyfenozide (runner 240 sc), a moulting accelerating compound (mac) currently submitted for registration in belgium, is an ipm (integrated pest management) compatible compound with strong, broad spectrum activity against lepidopterous pests in pome frunit and vegetables. field trials have confirmed reliable efficacy against larvae of winter moth o. brumata , both the overwintering and summer generation of the summer fruit tortrix moth, adoxophyes orana and also the tomato looper, chrysodeixe ...200315149109
characterization of adoxophyes honmai single-nucleocapsid nucleopolyhedrovirus: morphology, structure, and effects on larvae.a nucleopolyhedrovirus (npv) was isolated from a diseased larva of the smaller tea tortrix, adoxophyes honmai, collected from a tea field in tsukuba, ibaraki, japan. electron microscopic observations confirmed that a. honmai npv (adhonpv) was a single-nucleocapsid type virus. the genome size of adhonpv was estimated to be 111.6 +/- 0.9kb (mean +/- se) by restriction endonuclease analysis. adhonpv was also infectious to two other adoxophyes species, the summer fruit tortrix adoxophyes orana and a ...200312877827
the complete sequence of the adoxophyes orana granulovirus genome.the nucleotide sequence of the adoxophyes orana granulovirus (adorgv) dna genome was determined and analysed. the genome contains 99,657 bp and has an a + t content of 65.5%. the analysis predicted 119 orfs of 150 nucleotides or larger that showed minimal overlap. of these putative genes, 104 (87%) were homologous to genes identified previously in other baculoviruses. the mean overall amino acid identity of adorgv orfs was highest with cpgv orfs at 48%. sixty-three orfs were conserved among all ...200312842624
overwintering sites and winter mortality of euseius finlandicus (acari: phytoseiidae) in a peach orchard in northern greece.in peach orchards of northern greece euseius finlandicus oudemans overwinters in various sites on the trees, usually in groups of 5-15 females. overwintering females were mostly found in bark crevices near the hibernation cocoons of the peach moth adoxophyes orana (fischer von rosslerstamm), and also in small crevices of the bark and in pedicels left after fruit harvesting. empty scales of dead diaspidids and dead coccids, cocoons of lacewings and mummies of parasitized aphids sporadically found ...200212475073
phylogenetic analysis of conserved genes within the ecdysteroid udp-glucosyltransferase gene region of the slow-killing adoxophyes orana granulovirus.a physical map of the genome of adoxophyes orana granulovirus (aogv) was constructed for the restriction enzymes bamhi, bglii, ecori, psti and saci using restriction endonuclease analysis and dna hybridization techniques. this enabled the size of the aogv genome to be estimated at 100.9 kbp. a plasmid library covering 99.9% of the aogv genome was constructed using five restriction enzymes. the ecdysteroid udp-glucosyltransferase gene (egt) was located by hybridization with the egt gene of cydia ...200111514742
temperature dependent development of the parasitoid colpoclypeus florus (hymenoptera: eulophidae) in the laboratory.development, fecundity, and longevity of colpoclypeus florus (walker), a parasitoid of adoxophyes orana (fischer von röslerstamm) (lepidoptera: tortricidae), were studied under laboratory conditions at different constant temperatures. developmental time from egg to adult was inversely related to temperature and at 25 degrees c was 12.81 +/- 0.19 d. no adults emerged at 30 degrees c. the thermal units required for development from egg to adult were 232.56 +/- 19.5 degree-days. female c. florus ov ...200011142291
a trypsin inhibitor from snail medic seeds active against pest proteases.a protein trypsin inhibitor from seeds of snail medic (medicago scutellata), msti, has been purified by ion-exchange chromatography, gel-filtration chromatography and reverse-phase hplc. the protein inhibits the catalytic activity of bovine beta-trypsin, with an apparent kd of 1.8 x 10(-9), but exhibits no activity towards bovine alpha-chymotrypsin. moreover, msti inhibits the trypsin-like proteinase activity present in larvae of the crop pests adoxophyes orana, hyphantria cunea, lobesia botrana ...19979014368
sex pheromone ofadoxophyes orana: additional components and variability in ratio of (z)-9- and (z)-11-tetradecenyl acetate.twelve products related to the sex pheromone main components (z)-9- and (z)-11-tetradecenyl acetate (z9-14∶ac andz11-14∶ac, respectively), were identified in female pheromone gland extracts of the laboratory-reared summerfruit tortrix moth,adoxophyes orana f.v r. these are the geometric isomers and the alcohols of the main components, (z)-9-dodecenyl acetate, (z)-11-hexadecenyl acetate, and saturated acetates of 12-22 carbons. the ratio ofz9-14∶ac toz11-14∶ac in individuals varied from 3.5∶1 to ...198624306914
range of action and interaction of pheromone traps for the summerfruit tortrix moth,adoxophyes orana (f.v.r.).in a spindle-tree apple orchard, sex pheromone traps for the summerfruit tortrix moth were distributed at different spacings. from the captures, information was derived about influence of prevailing wind direction and of trap spacing on interactions between traps. prevailing wind direction in the experimental area had no appreciable influence on trap interaction, perhaps because of eddying within the planting. the relationship between density and capture of traps was first considered theoretical ...198224414731
characterization of the nuclear polyhedrosis virus dna of adoxophyes orana and of barathra brassicae. 1979373235
temperature-dependent juvenile hormone effects on pupation of adoxophyes orana. 19744443465
reproductive isolation of two tortricid moth species by different ratios of a two-component sex attractant.two tortricid mitoth species, adoxophyes orana and clepsis spectrana, utilize the samne two isomers, cis-9- and cis-l1-tetradecenyl acetate, as their sex attractant. commnlunication between the sexes of each species is separated effectively by a different blendinig of both compounds by the females and a different response to these blends by the males.197317806586
sex pheromones of summer fruit tortrix moth adoxophyes orana: two synergistic isomers.both cis-9-and cis-11-tetradecenyl acetate were isolated from 2500 virgin females of the summer fruit tortrix moth adoxophyes orana (f.v.r.) by gel permeation, column chromatography on silica gel, and gas chromatography. their chemical, physical, and biological properties were identical to those of the synthetic compounds. in contrast to the single compounds, mixtures of these pheromones were highly active in laboratory and field tests. catches, in traps, with the pheromone mixture were comparab ...197217842288
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