Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| danger lurking in the "unknowns": structure-to-function studies of hypothetical protein bleg1_2437 from bacillus lehensis g1 alkaliphile revealed an evolutionary divergent b3 metallo-beta-lactamase. | 2017 | 28175318 | |
| use of megaprimer and overlapping extension pcr (oe-pcr) to mutagenize and enhance cyclodextrin glucosyltransferase (cgtase) function. | protein engineering is a very useful tool for probing structure-function relationships in proteins. specifically, site-directed mutagenized proteins can provide useful insights into structural, binding and catalytic mechanisms of a protein, particularly when coupled with crystallization. in this chapter, we describe two protocols for performing site-directed mutagenesis of any protein-coding sequence, namely, megaprimer pcr and overlapping extension pcr (oe-pcr). we use as an example how these t ... | 2017 | 27709591 |
| data for proteome analysis of bacillus lehensis g1 in starch-containing medium. | bacillus lehensis g1 is a cyclodextrin glucanotransferase (cgtase) producer, which can degrade starch into cyclodextrin. here, we present the proteomics data of b. lehensis cultured in starch-containing medium, which is related to the article "proteome-based identification of signal peptides for improved secretion of recombinant cyclomaltodextrin glucanotransferase in escherichia coli" (ling et. al, in press). this dataset was generated to better understand the secretion of proteins involved in ... | 2017 | 28761915 |
| computational docking, molecular dynamics simulation and subsite structure analysis of a maltogenic amylase from bacillus lehensis g1 provide insights into substrate and product specificity. | maltogenic amylase (mag1) from bacillus lehensis g1 displayed the highest hydrolysis activity on β-cyclodextrin (β-cd) to produce maltose as a main product and exhibited high transglycosylation activity on malto-oligosaccharides with polymerization degree of three and above. these substrate and product specificities of mag1 were elucidated from structural point of view in this study. a three-dimensional structure of mag1 was constructed using homology modeling. docking of β-cd and malto-oligosac ... | 2016 | 27155296 |
| danger lurking in the "unknowns": structure-to-function studies of hypothetical protein bleg1_2437 from bacillus lehensis g1 alkaliphile revealed an evolutionary divergent b3 metallo-beta-lactamase. | the effectiveness of β-lactam antibiotics as chemotherapeutic agents to treat bacterial infections is gradually threatened with the emergence of antibiotic resistance mechanism among pathogenic bacteria through the production metallo-β-lactamase (mbl). in this study, we discovered a novel hypothetical protein (hp) termed bleg1_2437 from the genome of alkaliphilic bacillus lehensis g1 which exhibited mbl-like properties of b3 subclass; but evolutionary divergent from other circulating b3 mbls. do ... | 2016 | 27836972 |
| molecular characterization, modeling and docking of cyp107cb2 from bacillus lehensis g1, an alkaliphile. | cytochrome p450s are a superfamily of heme monooxygenases which catalyze a wide range of biochemical reactions. the reactions involve the introduction of an oxygen atom into an inactivated carbon of a compound which is essential to produce an intermediate of a hydroxylated product. the diversity of chemical reactions catalyzed by cytochrome p450s has led to their increased demand in numerous industrial and biotechnology applications. a recent study showed that a gene sequence encoding a cyp was ... | 2015 | 25766878 |
| enhanced degradation of pendimethalin by immobilized cells of bacillus lehensis xju. | a bacterium capable of degrading pendimethalin was isolated from the contaminated soil samples and identified as bacillus lehensis xju based on 16s rrna gene sequence analysis. 6-aminopendimethalin and 3,4-dimethyl 2,6-dinitroaniline were identified as the metabolites of pendimethalin degradation by the bacterium. the biodegradation of pendimethalin by freely suspended and the immobilized cells of b. lehensis on various matrices namely agar, alginate, polyacrylamide, and polyurethane foam was al ... | 2015 | 28324401 |
| purification and characterization of cyclodextrin β-glucanotransferase from novel alkalophilic bacilli. | the discovery of novel bacterial cyclodextrin glucanotransferase (cgtase) enzyme could provide advantages in terms of its production and relative activity. in this study, eight bacterial strains isolated from soils of a biodiversity-rich vegetation in egypt based on their hydrolyzing activity of starch, were screened for cgtase activity, where the most active strain was identified as bacillus lehensis. optimization process revealed that the using of rice starch (25%) and a mixture of peptone/yea ... | 2015 | 25362891 |
| the characterisation of an alkali-stable maltogenic amylase from bacillus lehensis g1 and improved malto-oligosaccharide production by hydrolysis suppression. | a maltogenic amylase (mag1) from alkaliphilic bacillus lehensis g1 was cloned, expressed in escherichia coli, purified and characterised for its hydrolysis and transglycosylation properties. the enzyme exhibited high stability at ph values from 7.0 to 10.0. the hydrolysis of β-cyclodextrin (β-cd) produced malto-oligosaccharides of various lengths. in addition to hydrolysis, mag1 also demonstrated transglycosylation activity for the synthesis of longer malto-oligosaccharides. the thermodynamic eq ... | 2014 | 25221964 |
| a sco protein among the hypothetical proteins of bacillus lehensis g1: its 3d macromolecular structure and association with cytochrome c oxidase. | at least a quarter of any complete genome encodes for hypothetical proteins (hps) which are largely non-similar to other known, well-characterized proteins. predicting and solving their structures and functions is imperative to aid understanding of any given organism as a complete biological system. the present study highlights the primary effort to classify and cluster 1202 hps of bacillus lehensis g1 alkaliphile to serve as a platform to mine and select specific hp(s) to be studied further in ... | 2014 | 24641837 |
| draft genome sequences of cyclodextrin-producing alkaliphilic bacillus strains jcm 19045, jcm 19046, and jcm 19047. | bacillus strains jcm 19045, jcm 19046, and jcm 19047 are alkaliphiles that produce β-cyclodextrin from starch. they are related to bacillus xiaoxiensis and bacillus lehensis. the genome information for these three strains will be useful for studies of the physiological role of cyclodextrin and cyclodextrin production. | 2014 | 24652985 |
| bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil. | a moderately halophilic bacterium, strain hna-14(t), was isolated from a saline-alkali soil sample collected in shache county, xinjiang province. on the basis of the polyphasic taxonomic data, the isolate was considered to be a member of the genus bacillus. the organism grew optimally at 30 °c and ph 8.0. it was moderately halophilic and its optimum growth occurred at 5-10% nacl. the diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid and the predominant menaquinone wa ... | 2014 | 25008165 |
| a comparative genomic analysis of the alkalitolerant soil bacterium bacillus lehensis g1. | bacillus lehensis g1 is a gram-positive, moderately alkalitolerant bacterium isolated from soil samples. b. lehensis produces cyclodextrin glucanotransferase (cgtase), an enzyme that has enabled the extensive use of cyclodextrin in foodstuffs, chemicals, and pharmaceuticals. the genome sequence of b. lehensis g1 consists of a single circular 3.99 mb chromosome containing 4017 protein-coding sequences (cdss), of which 2818 (70.15%) have assigned biological roles, 936 (23.30%) have conserved domai ... | 2014 | 24811681 |
| characteristics and applications of a recombinant alkaline serine protease from a novel bacterium bacillus lehensis. | a highly alkaline protease (blap) from a novel psychrotolerant and alkaliphilic bacterium, bacillus lehensis was cloned and expressed in escherichia coli. blap belongs to subtilase s8 family of proteases, comprising 27 aa secretion signal, 83 aa prosequence and 269 aa mature blap. the amino acids asp 141, his 171 and ser 324 form catalytic triad, while ile 214, leu 233 and asn 267 are other active site moieties. recombinant alkaline protease (rblap) is a monomeric protein of 39.0±1.0kda, and it ... | 2013 | 23340105 |
| bacillus xiaoxiensis sp. nov., a slightly halophilic bacterium isolated from non-saline forest soil. | a novel gram-stain-positive, slightly halophilic, catalase-positive, oxidase-negative, endospore-forming, motile, facultatively anaerobic, rod-shaped bacterium, designated strain jsm 081004t, was isolated from non-saline forest soil in xiaoxi national natural reserve, china. growth occurred with 0.5-20 % (w/v) nacl (optimum 2-4 %) at ph 6.0-10.5 (optimum ph 8.0) and at 5-40 °c (optimum 25-30°c). meso-diaminopimelic acid was present in the cell-wall peptidoglycan. the major cellular fatty acids w ... | 2010 | 20870888 |
| bacillus hunanensis sp. nov., a slightly halophilic bacterium isolated from non-saline forest soil. | a novel gram-stain-positive, slightly halophilic, catalase- and oxidase-positive, endospore-forming, motile, aerobic, rod-shaped bacterium, designated strain jsm 081003(t), was isolated from non-saline forest soil in hunan province, china. growth occurred with 0.5-15% (w/v) nacl (optimum 2-4%) at ph 6.5-10.5 (optimum ph 7.5-8.5) and at 5-40 °c (optimum 30 °c). meso-diaminopimelic acid was present in the cell-wall peptidoglycan. the major cellular fatty acids were iso-c15:0, anteiso-c15:0 and iso ... | 2010 | 20872176 |
| bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil. | a gram-positive, endospore-forming, alkalitolerant bacterial strain, designated mlb2t, was isolated from soil from leh, india, and was subjected to a polyphasic taxonomic study. the strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus bacillus. growth was observed at ph 7.0-11.0, but not at ph 6.0. the dna g+c content was 41.4 mol%. the highest level of 16s rrna gene sequence similarity was with bacillus oshimensis jc ... | 2007 | 17267957 |