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antimicrobial metal-based thiophene derived compounds.a novel series of thiophene derived schiff bases and their transition metal- [co(ii), cu(ii), zn(ii), ni(ii)] based compounds are reported. the schiff bases act as tridentate ligands toward metal ions via azomethine-n, deprotonated-n of ammine substituents and s-atom of thienyl moiety. the synthesized ligands along with their metal complexes were screened for their in vitro antibacterial activity against six bacterial pathogens (escherichia coli, shigella flexneri, pseudomonas aeruginosa, salmon ...201727766891
killing of diverse eye pathogens (acanthamoeba spp., fusarium solani, and chlamydia trachomatis) with alcohols.blindness is caused by eye pathogens that include a free-living protist (acanthamoeba castellanii, a. byersi, and/or other acanthamoeba spp.), a fungus (fusarium solani), and a bacterium (chlamydia trachomatis). hand-eye contact is likely a contributor to the spread of these pathogens, and so hand washing with soap and water or alcohol-based hand sanitizers (when water is not available) might reduce their transmission. recently we showed that ethanol and isopropanol in concentrations present in ...201728182670
rifampin enhances the activity of amphotericin b against fusarium solani species complex and aspergillus flavus species complex isolates from keratitis patients.the in vitro activities of amphotericin b in combination with rifampin were assessed against 95 ocular fungal isolates. the interactions between amphotericin b and rifampin at 4, 8, 16, and 32 μg/ml were synergistic for 11.8%, 51.0%, 90.2%, and 94.1%, respectively, of fusarium solani species complex isolates and for 13.6%, 45.5%, 93.2%, and 95.5%, respectively, of aspergillus flavus species complex isolates. antagonism was never observed for the amphotericin b-rifampin combinations.201728115342
ex vivo rabbit and human corneas as models for bacterial and fungal keratitis.in the study of microbial keratitis, in vivo animal models often require a large number of animals, and in vitro monolayer cell culture does not maintain the three-dimensional structure of the tissues or cell-to-cell communication of in vivo models. here, we propose reproducible ex vivo models of single- and dual-infection keratitis as an alternative to in vivo and in vitro models.201727844206
synthesis and biological evaluation of arylpyrazoles as fungicides against phytopathogenic fungi.3-phenol-1h-pyrazoles (2), 4-halogeno-3-phenol-1h-pyrazoles (3) and 2-(1-phenol-1h-pyrazol-5-yl)phenols (4) were prepared by the condensation of (e)-3-(dimethylamino)-1-phenylprop-2-en-1-ones and hydrazine hydrate or phenylhydrazine in good yields. they were evaluated against five phytopathogens fungi, namely cytospora sp., colletotrichum gloeosporioides, botrytis cinerea, alternaria solani and fusarium solani in vitro. most of the above-mentioned compounds exhibited activities. for example, 4-c ...201728247170
urnula sp., an endophyte of dicksonia antarctica, making a fragrant mixture of biologically active volatile organic compounds.urnula sp. was isolated as an endophyte of dicksonia antarctica and identified primarily on the basis of its its sequence and morphological features. the anamorphic state of the fungus appeared as a hyphomyceteous-like fungus as based on its features in culture and scanning electron microscopy examination of its spores. on potato dextrose agar (pda), the organism makes a characteristic fragrance resembling peach pie with vanilla overtones. a gc/ms analysis done on the volatile organic compounds ...201728188331
pathogenicity of conidiobolus coronatus and fusarium solani in mouse models.to study the pathogenicity of conidiobolus coronatus (c. coronatus) and fusarium solani (f. solani) in animal models. immunocompromised mice were treated with cyclophosphamide and prednisolone via intraperitoneal injection before and after inoculation. according to pathogenic characteristics of different fungi, c. coronatus was used to infect mice via intravenous inoculation, intraperitoneal inoculation, gastrointestinal infusion and intradermal inoculation methods. and f. solani was used to inf ...201728239908
trialkylamine derivatives containing a triazole moiety as promising ergosterol biosynthesis inhibitor: design, synthesis, and antifungal activity.as a part of our continuing research on amine derivative antifungal agents, 19 novel target compounds containing 1,2,4-triazole and tertiary amine moieties were designed and synthesized, and their in vitro antifungal activities against six phytopathogenic fungi (magnaporthe grisea, alternaria solani, fusarium solani, curvularia lunata, a. alternata, f. graminearum) were assayed. all target compounds were elucidated by means of (1)h-nmr, (13)c-nmr, high resolution (hr)-ms, and ir analysis. the re ...201728049918
a novel tantalum-containing bioglass. part ii. development of a bioadhesive for sternal fixation and repair.with over a million median sternotomy surgeries performed worldwide every year, sternal wound complications have posed a serious risk to the affected patients. a rigid therapeutic sternal fixation device has therefore become a necessity. in this work, the incorporation of up to 0.5mol% of tantalum pentoxide (ta2o5), in exchange for zinc oxide (zno), into the sio2-zno-cao-sro-p2o5 glass system is presented. the effect of ta incorporation on the physical, chemical and biological properties of the ...201727987724
prospecting fungal parasites of the potato cyst nematode globodera pallida using a rapid screening technique.seven filamentous fungal species were isolated from individual eggs of globodera pallida cysts collected from infested fields in shelley idaho, usa and identified as chaetomium globosum, fusarium oxysporum, fusarium solani, fusarium tricinctum, microdochium bolleyi, purpureocillium lilacinum, and plectosphaerella cucumerina. their ability to reduce infection by g. pallida in planta were assessed in simple, reproducible micro-rhizosphere chambers (micro-rocs). all fungi reduced g. pallida infecti ...201728375550
screening of invasive fungal infections by a real-time panfungal (pan-acf) polymerase chain reaction assay in patients with haematological malignancy.invasive fungal infection (ifi) is a fatal infection in haematology patients. there is an urgent need for reliable screening methods facilitating timely diagnosis and treatment. a real-time panfungal polymerase chain reaction (pcr) assay based on taqman technology targeting 18s ribosomal rna gene was used to screen whole blood specimen obtained from series of haematology malignancy patients for ifis.201728303817
multidrug-resistant fusarium in keratitis: a clinico-mycological study of keratitis infections in chennai, india.in this study, we aimed to present the first molecular epidemiological data from chennai, india, analyse keratitis cases that have been monitored in a university hospital during 2 years, identify the responsible fusarium species and determine antifungal susceptibilities. a total of 10 cases of keratitis were included in the study. fusarium isolates were identified using the second largest subunit of the rna polymerase gene (rpb2) and the translation elongation factor 1 alpha (tef1). antifungal s ...201727766684
rapid and sensitive diagnoses of dry root rot pathogen of chickpea (rhizoctonia bataticola (taub.) butler) using loop-mediated isothermal amplification assay.dry root rot (drr) caused by the fungus rhizoctonia bataticola (taub.) butler, is an emerging disease in chickpea. the disease is often mistaken with other root rots like fusarium wilt, collar rot and black root rot in chickpea. therefore, its timely and specific detection is important. current detection protocols are either based on mycological methods or on protocols involving dna amplification by polymerase chain reaction (pcr). here we report the rapid and specific detection of r. bataticola ...201728218268
[fusarium species associated with basal rot of garlic in north central mexico and its pathogenicity].garlic in mexico is one of the most profitable vegetable crops, grown in almost 5,451ha; out of which more than 83% are located in zacatecas, guanajuato, sonora, puebla, baja california and aguascalientes. blossom-end rot caused by fusarium spp is widely distributed worldwide and has been a limiting factor in onion and garlic production regions, not only in mexico but also in other countries. the presence of fusarium oxysporum has been reported in guanajuato and aguascalientes. fusarium culmorum ...201727590608
comparison of dna microarray, loop-mediated isothermal amplification (lamp) and real-time pcr with dna sequencing for identification of fusarium spp. obtained from patients with hematologic malignancies.the performance of three molecular biology techniques, i.e., dna microarray, loop-mediated isothermal amplification (lamp), and real-time pcr were compared with dna sequencing for properly identification of 20 isolates of fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. dna microarray, lamp and real-time pcr identified 16 (80%) out of 20 samples as fusarium solani species complex (fssc) and four (20%) as fusarium spp. t ...201728324245
a novel peroxidase purified from marsdenia megalantha latex inhibits phytopathogenic fungi mediated by cell membrane permeabilization.an antifungal class iii peroxidase was purified from marsdenia megalantha latex (named mo-pox) using deae-cellulose and gel filtration chromatography on a superose 12 hr 10/30 column. mm-pox has an apparent molecular mass of 67.0kda and a pi of 5.2, shares identity with other peroxidases, and follows michaelis-menten kinetics. it has a high affinity for guaiacol and hydrogen peroxide. the ph and temperature optima for mm-pox were 5.0-7.0 and 60°c, respectively. the catalytic activity of mm-pox w ...201728057569
epidemiological study of fusarium species causing invasive and superficial fusariosis in japan.in japan, fusarium species are known etiological agents of human fungal infection; however, there has been no report of a large-scale epidemiological study on the etiological agents of fusariosis. a total of 73 fusarium isolates from patients with invasive fusariosis (if, n= 36) or superficial fusariosis (sf, n= 37), which were obtained at hospitals located in 28 prefectures in japan between 1998 and 2015, were used for this study. fusarium isolates were identified using fusarium- and fusarium s ...201728250364
agarwood formation induced by fermentation liquid of lasiodiplodia theobromae, the dominating fungus in wounded wood of aquilaria sinensis.agarwood is broadly used in incense and medicine. traditionally, agarwood formation is induced by wounding the trunks and branches of some species of aquilaria spp., including a. sinensis. as recently evidenced, some fungi or their fermentation liquid may have the potential of inducing agarwood formation. the present study aimed to analyze the fungi isolated from an agarwood-producing a. sinensis tree and subsequently identify the fungi capable of promoting agarwood formation. we identified a to ...201728224223
discovery of anti-microbial and anti-tubercular molecules from fusarium solani: an endophyte of glycyrrhiza glabra.glycyrrhiza glabra is a high-value medicinal plant thriving in biodiversity rich kashmir himalaya. the present study was designed to explore the fungal endophytes from g. glabra as a source of bioactive molecules.201728150900
[confocal microscopy as an early relapse marker after keratoplasty due to fusarium solani keratitis].in the case of therapy-resistant keratitis an infection with fusarium solani should be taken into consideration as a rare but very severe eye disease. in the majority of cases fusarium solani keratitis will result in a protracted clinical course despite aggressive medicinal and surgical interventions. we describe the case of a referred patient after intensive topical, intracameral and systemic antibacterial and antimycotic therapy as well as surgical treatment with emergency keratoplasty à chaud ...201727260622
fast activated charcoal prepurification of fusarium solani β-glucosidase for an efficient oleuropein bioconversion.fungal β-glucosidases were extensively studied regarding their various potential biotechnology applications. here, we report the selection of fusarium solani strain producing high yield of β-glucosidase activity. the effect of some factors on β-glucosidase production was studied including: initial ph, medium composition, concentration of carbon and nitrogen sources, and particle size of raw substrates. the optimal enzyme production was obtained with 4 units of ph. the highest β-glucosidase activ ...201727340756
antimicrobial activity and mechanism of action of a thionin-like peptide from capsicum annuum fruits and combinatorial treatment with fluconazole against fusarium solani.many fusarium species are able to cause severe infections in plants as well as in animals and humans. therefore, the discovery of new antifungal agents is of paramount importance. cathi belongs to the thionins, which are cationic peptides with low molecular weights (∼ 5 kda) that have toxic effects against various microorganisms. herein, we study the mechanism of action of cathi and its combinatory effect with fluconazole (flc) against fusarium solani. the mechanism of action of cathi was studie ...201728073158
kinetic behaviour of recombinant fusarium solani lipases using monomolecular films: effect of the heterologous expression.two lipases from fusarium solani, fsl and fsl2, were efficiently expressed in pichia pastoris. to check the influence of the expression on interfacial properties of fsl and to study kinetic properties of fsl2, interfacial parameters of fsl2, native fsl, untagged recombinant and tagged recombinant forms of fsl were compared using the monomolecular film technique. kinetic study on the dependence of the stereoselectivity of these lipases on the surface pressure was performed using three dicaprin is ...201727746351
efficient heterologous expression of fusarium solani lipase, fsl2, in pichia pastoris, functional characterization of the recombinant enzyme and molecular modeling.the gene coding for a lipase of fusarium solani, designated as fsl2, shows an open reading frame of 906bp encoding a 301-amino acid polypeptide with a molecular mass of 30kda. based on sequence similarity with other fungal lipases, fsl2 contains a catalytic triad, consisting of ser144, asp198, and his256. fsl2 cdna was subcloned into the pgapzαa vector containing the saccharomyces cerevisiae α-factor signal sequence and this construct was used to transform pichia pastoris and achieve a high-leve ...201727620466
[clusters of fusarium solani infection in juvenile captive born caretta caretta sea turtles].various yeasts and filamentous fungi are described as the cause of infection in sea turtles. among them, fusarium solani is responsible both for superficial and invasive infection in weakened adults (capture, stranding), and wild nest contamination, causing massive losses during hatching. we illustrate the pathogenicity of this fungus in sea turtles, through our experience with the species caretta caretta (loggerhead turtle) and its reproduction, which was obtained for the first time in 2010 at ...201728043787
high-throughput analysis of enzymatic hydrolysis of biodegradable polyesters by monitoring cohydrolysis of a polyester-embedded fluorogenic probe.biodegradable polyesters have the potential to replace nondegradable, persistent polymers in numerous applications and thereby alleviate plastic accumulation in the environment. herein, we present an analytical approach to study enzymatic hydrolysis of polyesters, the key step in their overall biodegradation process. the approach is based on embedding fluorescein dilaurate (fdl), a fluorogenic ester substrate, into the polyester matrix and on monitoring the enzymatic cohydrolysis of fdl to fluor ...201728140581
formation of 2,4-d bound residues in soils: new insights into microbial metabolism.the microbial contribution to the formation of bound residues in soils is studied by characterizing the metabolic activity of three microorganisms (trametes versicolor, fusarium solani and ralstonia eutropha) on (14)c-2,4-dichlorophenoxyacetic acid (2,4-d) during incubation in synthetic liquid media and soil. a fractionation protocol was applied to quantify the (14)c-2,4-d that was incorporated into the biomass among biomolecular-like fractions. successive fractionation of microbial biomass was ...201728131449
cleavage product accumulation decreases the activity of cutinase during pet hydrolysis.the fusarium solani cutinase (fsc) is a promising candidate for the enzymatic degradation of the synthetic polyester polyethylene terephthalate (pet) but still suffers from a lack of activity. using atomic md simulations with different concentrations of cleavage product ethylene glycol (eg), we show influences of eg on the dynamic of fsc. we observed accumulation of eg in the active site region reducing the local flexibility of fsc. furthermore, we used a coarse-grained mechanical model to inves ...201728128951
trans-glycosylation capacity of a highly glycosylated multi-specific β-glucosidase from fusarium solani.an extracellular β-glucosidase from fusaruim solani cultivated on wheat bran was purified by only two chromatographic steps. the purified enzyme exhibited optimal temperature and ph at 60 °c and ph 5, respectively. the purified β-glucosidase behaves as a very large protein due to its high degree of glycosylation. more interestingly, the endoglycosidase h (endo h) treatment led to 97.55% loss of its initial activity after 24 h of treatment. besides, the addition of tunicamycin (nucleoside antibio ...201727988803
does a triple combination have better activity than double combinations against multiresistant fungi? experimental in vitro evaluation.in this study, the in vitro interactions of amphotericin b (amb), voriconazole (vrc) and anidulafungin (afg) in double and triple combinations against four species of multiresistant fungi (fusarium solani, lomentospora prolificans, scopulariopsis brevicaulis and scopulariopsis brumptii) were evaluated. in general, amb combined with afg was the most synergistic, especially against f. solani (7/8; 87.5%) when low concentrations of amb were used, i.e. 0.125-0.5 µg/ml. the least active combination w ...201728257903
cytotoxic naphthoquinone and azaanthraquinone derivatives from an endophytic fusarium solani.bioactivity-guided fractionation of the ethyl acetate extract obtained from the culture of the endophytic fungus fusarium solani resulted in the isolation of one new naphthoquinone, 9-desmethylherbarine (1), and two azaanthraquinone derivatives, 7-desmethylscorpinone (2) and 7-desmethyl-6-methylbostrycoidin (3), along with four known compounds. their structures were elucidated by spectral analysis, as well as a direct comparison of spectral data with those of known compounds. azaanthraquinones 2 ...201728257197
chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans.the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ...201728725151
two cases of fusarium solani (mart) sacc. infection of human finger nails.fusarium solani, not known to produce onychomycosis, was repeatedly recovered trom two healthy men with'iesions in fingernails. mycelial fragments and microconidia were observed in the diseased nails and the fungus was repeatedly isolated in the cyclo-heximide-free culture medium. the report indicates that many fungi, hitherto considered nonpathogens may still be able to colonize a variety of human tissues.201728193949
antibacterial secondary metabolites from an endophytic fungus, fusarium solani jk10.extensive chemical investigation of the endophytic fungus, fusarium solani jk10, harbored in the root of the ghanaian medicinal plant chlorophora regia, using the osmac (one strain many compounds) approach resulted in the isolation of seven new 7-desmethyl fusarin c derivatives (1-7), together with five known compounds (8-12). the structures of the new compounds were elucidated by analysis of their spectroscopic data including 1d, 2d nmr, hresi-ms(n) and ir data. the relative configuration of co ...201728392268
isolation, identification, optimization, and metabolite profiling of streptomyces sparsus vsm-30.deep sea sediment samples of bay of bengal (visakhapatnam) have been analyzed for actinomycetes as an elite source to screen for the production of bioactive metabolites. the actinomycetes strain vsm-30 has an exciting bioactivity profile and was isolated during our systemic screening of marine actinomycetes. it was identified as streptomyces sparsus based on morphological, physiological, biochemical, and molecular approaches. response surface methodology regression analysis was carried out to fi ...201728669076
isolation and identification of fungi associated with spoilt fruits vended in gwagwalada market, abuja, nigeria.annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. this study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in gwagwalada market, abuja, and recommend appropriate control measure.201728507410
application and comparison in biosynthesis and biodegradation by fusarium solani and aspergillus fumigatus cutinases.in this study, two synthesized cutinase genes from fusarium solani and aspergillus fumigatus were expressed in pichia pastoris x33. the characteristics of these two cutinases were investigated and compared. the results indicated that f. solani and a. fumigatus cutinases hydrolyzed p-nitrophenyl substrates with different carbon chain lengths. a. fumigatus cutinase predominately hydrolyzed p-nitrophenyl butyrate, but f. solani cutinase preferred p-nitrophenyl decanoate. the abilities of polymer sy ...201728673841
fusarium keratitis in germany.fusarium keratitis is a destructive eye infection that is difficult to treat and which results in poor outcome. in the tropical and subtropical areas the infection is relatively common and associated with trauma or chronic eye diseases. however, in recent years, an increased incidence has been reported in temperate climate regions. at the german national reference center, we observed a steady increase in case numbers since 2014. here, we present the first german case series of eye infections wit ...201728747368
a sugarcane pathogenesis-related protein, scpr10, plays a positive role in defense responses under sporisorium scitamineum, srmv, sa, and meja stresses.a pathogenesis-related gene, scpr10 , was isolated from sugarcane and its bio-function was characterized, demonstrating that scpr10 was involved in plant defense responses to sporisorium scitamineum , srmv, sa, and meja stresses. plant fungal and viral diseases are the major concerns in sugarcane industry. many anti-fungal and antivirus components, including pathogenesis-related (pr) proteins, have been identified. the pathogenesis-related protein 10 (pr10) is the dominant group in pr families, ...201728634719
flow cytometry is a powerful tool for assessment of the viability of fungal conidia in metalworking fluids.fungal contamination of metalworking fluids (mwf) is a dual problem in automated processing plants because resulting fungal biofilms obstruct cutting, drilling, and polishing machines. moreover, some fungal species of mwf comprise pathogens such as fusarium solani therefore, the development of an accurate analytical tool to evaluate conidial viability in mwf is important. we developed a flow cytometric method to measure fungal viability in mwf using f. solani as the model organism. to validate t ...201728625992
successful salvage chemotherapy and allogeneic transplantation of an acute myeloid leukemia patient with disseminated fusarium solani infection.disseminated fusarium infection is associated with high mortality in immunocompromised patients. patients with acute myeloid leukemia (aml) often have an extended duration of neutropenia during intensive induction chemotherapy, consolidation chemotherapy, and hematopoietic stem cell transplantation (sct). there is no consensus regarding management of invasive disseminated fusarium infections in the setting of prolonged neutropenia (tortorano et al., 2014) [1]. we report a case of disseminated fu ...201728794968
scmed7, a sugarcane mediator subunit gene, acts as a regulator of plant immunity and is responsive to diverse stress and hormone treatments.the mediator complex, is an essential component of the rna polymerase ii general transcriptional machinery in eukaryotes. mediator subunit 7 (med7), a key subunit in the central module of this complex, plays an important role in gene transcriptional regulation. the present study isolated the full-length cdna of the med7 gene of sugarcane, hereby designated as scmed7, which was characterized to harbor a 525-bp open reading frame that is predicted to encode a 174-amino acid protein with a molecula ...201728785867
melanization of fusarium keratoplasticum (f. solani species complex) during disseminated fusariosis in a patient with acute leukemia.fusarium spp. are recognized as the second most frequently filamentous fungi causing opportunistic infections and particularly important due to the increasing number of immunocompromised patients. f. keratoplasticum (a member of f. solani species complex) is one of the fusarium species commonly associated with human infection, and therefore, studies on the virulence of this fungus are needed. this study aimed to confirm the presence of melanin in f. keratoplasticum from a patient with systemic f ...201728616680
enzymatic hydrolysis of polyester thin films at the nanoscale: effects of polyester structure and enzyme active-site accessibility.biodegradable polyesters have a large potential to replace persistent polymers in numerous applications and to thereby reduce the accumulation of plastics in the environment. ester hydrolysis by extracellular carboxylesterases is considered the rate-limiting step in polyester biodegradation. in this work, we systematically investigated the effects of polyester and carboxylesterase structure on the hydrolysis of nanometer-thin polyester films using a quartz-crystal microbalance with dissipation m ...201728538100
[fusarioses to fusarium solani in an immunocompetent and immunocompromised diagnosed in military hospital of rabat].fusarium are ubiquitous hyalohyphomycoses, usually encountered in the soil. they are the second unusual fungal pathogens after the trichosporon. intertrigo fusarium sp. is a rare achievement. we report a case of intertrigos interorteils in an immunocompetent 45years old and a same case associated with a total onychodystrophy in an immunocompromised 75year-old. laboratory diagnosis has found fusarium solani confirmed with the positivity of a pure culture twice. good progress was noted with terbin ...201728478967
intracellular siderophore detection in an egyptian, cobalt-treated f. solani isolate using sem-edx with reference to its tolerance.an egyptian, plant pathogenic fusarium solani isolate was grown on cobalt concentrations of 0, 50, 200, 500, 800, and 1000 ppm. the isolate survived concentrations up to 800 ppm, however failed to grow at 1000 ppm. morphology and elemental analysis of the isolate under the investigated co concentrations were studied using scanning electron microscopy (sem) and energy dispersive x-ray microanalysis (edx). the isolate reserved its morphology up to a concentration of 200 ppm. morphological distorti ...201728735319
phytochemistry, antioxidant potential and antifungal of byrsonima crassifolia on soil phytopathogen control.the use of chemical defensives to control fungal diseases has by consequence to impact negatively over the environment and human health, this way, the use of plant extracts with antifungal properties along with proper cultural management makes viable an alternative plant production control, specially for familiar and organic cultures. the objective of this study was to perform phytochemical and antioxidant analysis of byrsonima crassifolia (canjiqueira) barks and evaluate its antifungal potentia ...201728699968
in vitro nematophagous activity of predatory fungi on infective nematodes larval stage of strongyloidae family.the main goal of the present research conducted to assess the in vitro activity of the nematophagous fungi duddingtonia flagrans, fusarium solani, verticillium chlamidosporium, and trichoderma harzianum.201728698742
role of pattern recognition receptors in the modulation of antimicrobial peptide expression in the corneal epithelial innate response to f. solani.fusarium solani (f. solani) keratitis is a potentially sight-threatening fungal infection of the cornea. antimicrobial peptides (amps), such as human β-defensins (hbds) and cathelicidins, essential components of the immune system, likely have a protective role against f. solani keratitis. we examined the role of pattern recognition receptors (prrs), dectin-1, and tlr2 in f. solani-induced modulation of amp expression in vitro.201728460048
non-host resistance: dna damage is associated with sa signaling for induction of pr genes and contributes to the growth suppression of a pea pathogen on pea endocarp tissue.salicylic acid (sa) has been reported to induce plant defense responses. the transcriptions of defense genes that are responsible for a given plant's resistance to an array of plant pathogens are activated in a process called non-host resistance. biotic signals capable of carrying out the activation of pathogenesis-related (pr) genes in pea tissue include fungal dnase and chitosan, two components released from fusarium solani spores that are known to target host dna. recent reports indicate that ...201728421088
morphological and molecular characterization of fusarium spp. associated with olive trees dieback in tunisia.dieback and wilting symptoms caused by complex soilborne fungi are nowadays the most serious threatening disease affecting olive trees (olea europaea) in tunisia and presumably in many mediterranean basin countries. fusarium is one of the important phytopathogenic genera associated with dieback symptoms of olive trees. the objective of the present study was to confirm the pathogenicity of fusarium spp. isolated from several olive-growing areas in tunisia. according to the pathogenic test done on ...201728401468
nonsteroidal anti-inflammatory drugs (nsaids) inhibit the growth and reproduction of chaetomium globosum and other fungi associated with water-damaged buildings.indoor mold due to water damage causes serious human respiratory disorders, and the remediation to homes, schools, and businesses is a major expense. prevention of mold infestation of building materials would reduce health problems and building remediation costs. nonsteroidal anti-inflammatory drugs (nsaids) inhibit yeasts and a limited number of filamentous fungi. the purpose of this research was to determine the possible inhibitory activity of nonsteroidal anti-inflammatory drugs (nsaids) on g ...201728795317
engineered humicola insolens cutinase for efficient cellulose acetate deacetylation.cutinases comprise a family of esterases with broad hydrolytic activity for chain and pendant ester groups. this work aimed to identify and improve an efficient cutinase for cellulose acetate (ca) deacetylation. the development of a mild method for ca fiber surface deacetylation will result in improved surface hydrophilicity and reactivity while, when combined with cellulases, a route to the full recycling of ca to acetate and glucose. in this study, the comparative ca deacetylation activity of ...201728488758
biodegradation of benzo[α]pyrene, toluene, and formaldehyde from the gas phase by a consortium of rhodococcus erythropolis and fusarium solani.polycyclic aromatic hydrocarbons (pahs) and volatile organic compounds (vocs) are important indoor contaminants. their hydrophobic nature hinders the possibility of biological abatement using biofiltration. our aim was to establish whether the use of a consortium of fusarium solani and rhodococcus erythropolis shows an improved performance (in terms of mineralization rate and extent) towards the degradation of formaldehyde, as a slightly polar voc; toluene, as hydrophobic voc; and benzo[α]pyrene ...201728685193
inhibition of fusarium solani infection in murine keratocytes by lactobacillus salivarius ssp. salivarius jcm1231 culture filtrate in vitro.to explore the inhibitory activity of lactobacillus salivarius ssp. salivarius jcm1231 (l. salivarius jcm1231) culture filtrate against fusarium solani (f. solani) and its effects on murine keratocytes (mks) infected with f. solani.201728636459
attempt to simultaneously generate three chiral centers in 4-hydroxyisoleucine with microbial carbonyl reductases.a panel of microorganisms was screened for selective reduction ability towards a racemic mixture of prochiral 2-amino-3-methyl-4-ketopentanoate (rac-amkp). several of the microorganisms tested produced greater than 0.5mm 4-hydroxyisoleucine (hil) from rac-amkp, and the stereoselectivity of hil formation was found to depend on the taxonomic category to which the microorganism belonged. the enzymes responsible for the amkp-reducing activity, apar and fsar, were identified from two of these microor ...201728698052
chlorogenic acid is a fungicide active against phytopathogenic fungi.plants synthesize diverse types of secondary metabolites and some of them participate in plant protection against pathogen attack. these compounds are biodegradable and renewable alternatives, which may be envisaged for the control of plant pests and diseases. chlorogenic acid (cga) is a phenolic secondary metabolite which accumulates in diverse plant tissues and can be found in several agro-industrial by-products and waste. the aim of this work was to determine whether cga could control the gro ...201728755691
the antibiotic polymyxin b exhibits novel antifungal activity against fusarium species.the genus fusarium comprises many species, including fusarium oxysporum, fusarium solani, fusarium graminearum and fusarium verticillioides, and causes severe infections in plants and humans. in clinical settings, fusarium is the third most frequent mould to cause invasive fungal infections after aspergillus and the mucorales. f. solani and f. oxysporum are the most prevalent fusarium spp. causing clinical disease. however, few effective antifungal drugs are available to treat human and plant fu ...201728433743
endophytic fungi harbored in panax notoginseng: diversity and potential as biological control agents against host plant pathogens of root-rot disease.endophytic fungi play an important role in balancing the ecosystem and boosting host growth. in the present study, we investigated the endophytic fungal diversity of healthy panax notoginseng and evaluated its potential antimicrobial activity against five major phytopathogens causing root-rot of p. notoginseng.201728701877
genetic diversity and antifungal susceptibility of fusarium isolates in onychomycosis.fusarium species have emerged as an important human pathogen in skin disease, onychomycosis, keratitis and invasive disease. onychomycosis caused by fusarium spp. the infection has been increasingly described in the immunocompetent and immunosuppressed hosts. considering onychomycosis is a difficult to treat infection, and little is known about the genetic variability and susceptibility pattern of fusarium spp., further studies are necessary to understand the pathogenesis and better to define th ...201728657120
cluster of fusarium solani isolations in a bronchoscopy unit. 201626417853
cd34+ highly enriched allogeneic stem cell transplantation in a patient with mixed phenotype acute leukemia and fusarium solani sepsis. 201626382275
reprint of: effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte fusarium solani.volumetric productivity of camptothecin from the suspension culture of the endophyte fusarium solani was enhanced up to ∼152 fold (from 0.19μgl(-1)d(-1) to 28.9μgl(-1)d(-1)) under optimized fermentation conditions including initial ph (6.0), temperature (32°c) and agitation speed (80rpm) with (5% (v/v)) ethanol as medium component. among various elicitors and precursors studied, tryptamine (0.5mm) as precursor and bovine serum albumin (bsa) (0.075mm) as an elicitor added on day 6 of the cultivat ...201627189536
time-dependent matrix metalloproteinases and tissue inhibitor of metalloproteinases expression change in fusarium solani keratitis.to investigate matrix metalloproteinases (mmps) and tissue inhibitor of metalloproteinases (timps) expression during the progress of fusarium solani (f.solani) keratitis in a rat model.201627162721
raman spectroscopy analysis of the biochemical characteristics of experimental keratomycosis.to investigate the biochemical characteristics in experimental keratomycosis by raman spectroscopy analysis in vitro and in vivo.201627158983
production of naphthoquinones and phenolics by a novel isolate fusarium solani psc-r of palk bay and their industrial applications.the present study was attempted to enhance the production of naphthoquinones and phenolics by fusarium solani psc-r of palk bay origin, which exhibited potent antibacterial, antioxidant and dyeing activity. maximum productivity of naphthoquinones and phenolics was achieved in potato infusion medium supplemented with 2% sucrose. addition of nitrogen sources to the medium adversely affected the production of both naphthoquinones and phenolics. an initial ph of 5 and incubation at 31°c for six days ...201627156595
ag doped hollow tio2 nanoparticles as an effective green fungicide against fusarium solani and venturia inaequalis phytopathogens.chemical-based pesticides are widely used in agriculture to protect crops from insect infestation and diseases. however, the excessive use of highly toxic pesticides causes several human health (neurological, tumor, cancer) and environmental problems. therefore nanoparticle-based green pesticides have become of special importance in recent years. the antifungal activities of pure and ag doped (solid and hollow) tio2 nanoparticles are studied against two potent phytopathogens, fusarium solani (wh ...201626808118
fusarium solani endocarditis in an acute leukemia patient. 201626706407
fusarium paranaense sp. nov., a member of the fusarium solani species complex causes root rot on soybean in brazil.isolates of fusarium obtained from soybean plants showing symptoms of root rot collected in subtropical southern and tropical central brazil were characterized based on phylogenetic analyses, sexual crossing, morphology, and pathogenicity tests. a novel species within the fusarium solani species complex (fssc) causing soybean root rot is formally described herein as fusarium paranaense. this species can be distinguished from the other soybean root rot pathogens in the fssc, which are commonly as ...201626693684
therapeutic effect of intrastromal voriconazole, topical voriconazole, and topical natamycin on fusarium keratitis in rabbit.purpose. evaluating the therapeutic effect of topical and intrastromal voriconazole and topical natamycin on fusarium keratitis. methods. 24 rabbits were selected. the stroma of their corneas was inoculated with suspension of fusarium solani species complex. seven days after injection they were divided into 4 groups randomly: the first group was treated with topical voriconazole (tv) 1% for one week, the second one with one-time intrastromal injection of voriconazole (isv) 50 microgram/0.1 ml, a ...201627298734
fungal associates of the xylosandrus compactus (coleoptera: curculionidae, scolytinae) are spatially segregated on the insect body.studies of symbioses have traditionally focused on explaining one-to-one interactions between organisms. in reality, symbioses are often much more dynamic. they can involve many interacting members, and change depending on context. in studies of the ambrosia symbiosis-the mutualism between wood borer beetles and fungi-two variables have introduced uncertainty when explaining interactions: imprecise symbiont identification, and disregard for anatomical complexity of the insects. the black twig bo ...201627357160
genotyping and in vitro antifungal susceptibility testing of fusarium isolates from onychomycosis in india.onychomycosis refers to fungal infection of the nail and is commonly caused by dermatophytes, while yeasts and non-dermatophytic molds (ndm) are increasingly recognized as pathogens in nail infections. the present study was done to delineate molecular epidemiology of fusarium onychomycosis in india. five hundred nail samples of indian patients clinically suspected of onychomycosis were subjected to direct microscopy and fungal culture. representative fusarium isolates were further identified to ...201627138574
identification of fusarium solani species complex from infected zebrafish (danio rerio).although fusarium sp. infections have been reported in aquatic invertebrates, studies of fusarium spp. as fish pathogens remain very limited. in our study, a fungus was isolated from diseased zebrafish (danio rerio). dna sequence analysis of the fungus, based on a partial region of the translation elongation factor 1α gene (ef-1α), the internal transcribed spacer region and domains d1 and d2 of the large subunit of the ribosomal rna gene (its plus lsu), and the rna polymerase ii subunit gene (rp ...201627664098
molecular and pathological characterization of fusarium solani species complex infection in the head and lateral line system of sphyrna lewini.a severe fungal infection affecting the head and lateral line system was diagnosed in 7 captive scalloped hammerhead sharks sphyrna lewini in an aquarium in thailand. extensive and severe necrotizing cellulitis was consistently observed microscopically along the cephalic and lateral line canals in conjunction with positive fungal cultures for fusarium sp. molecular phylogenetic analysis was performed from 3 isolates based on the nucleotide sequences containing internally transcribed spacer (its) ...201627503915
genome sequence of fusarium isolate mya-4552 from the midgut of anoplophora glabripennis, an invasive, wood-boring beetle.the fusarium solani species complex (fssc) is a clade of environmentally ubiquitous fungi that includes plant, animal, and insect associates. here, we report the draft genome sequence of the undescribed species fssc 6 (isolate mya-4552), housed in the gut of the wood-boring cerambycid beetle anoplophora glabripennis.201627445364
identifying the role of reactive oxygen species (ross) in fusarium solani spores inactivation.the inactivation mechanism of photocatalytic disinfectants on bacteria is well known. in contrast, the potential inactivation of fungal spores by visible-light induced photocatalysis has been recognized, but the inactivation mechanism is poorly understood. we hypothesize that photocatalytically generated reactive oxygen species (ross) are directly involved in this mechanism. to test this hypothesis, we identified the roles of ross in the inactivation of fusarium solani spores. as the photocataly ...201627696305
elucidating the key role of the fungal mycelium on the biodegradation of n-pentane as a model hydrophobic voc.the role of the aerial mycelium of the fungus fusarium solani in the biodegradation of n-pentane was evaluated in a continuous fungal bioreactor (fb) to determine the contribution of the aerial (hyphae) and non-aerial (monolayer) fungal biomass. the experimental results showed that although the aerial biomass fraction represented only 25.9(±3)% on a dry weight basis, it was responsible for 71.6(±4)% of n-pentane removal. the fb attained a maximum elimination capacity (ecmax) of 680(±30) g m(-3)  ...201627209557
fusarium solani subretinal abscess in a patient with acute myeloid leukemia.to report on a case of fusarium solani subretinal abscess in a patient with acute myeloid leukemia treated with an allogenic bone marrow transplant.201627828901
endophytic filamentous fungi from a catharanthus roseus: identification and its hydrolytic enzymes.this paper reported on the various filamentous fungi strains that were isolated from a wild grown catharanthus roseus. based on the morphological characteristics and molecular technique through a polymerase chain reaction and dna sequencing method using internal transcribed spacer (its), these fungi had been identified as a colletotrichum sp., macrophomina phaseolina, nigrospora sphaerica and fusarium solani. the ultrastructures of spores and hyphae were observed under a scanning electron micros ...201627275114
photodynamic treatment with phenothiazinium photosensitizers kills both ungerminated and germinated microconidia of the pathogenic fungi fusarium oxysporum, fusarium moniliforme and fusarium solani.the search for alternatives to control microorganisms is necessary both in clinical and agricultural areas. antimicrobial photodynamic treatment (apdt) is a promising light-based approach that can be used to control both human and plant pathogenic fungi. in the present study, we evaluated the effects of photodynamic treatment with red light and four phenothiazinium photosensitizers (ps): methylene blue (mb), toluidine blue o (tbo), new methylene blue n (nmbn) and the phenothiazinium derivative s ...201627623154
genotyping of fusarium isolates from onychomycoses in colombia: detection of two new species within the fusarium solani species complex and in vitro antifungal susceptibility testing.fusariosis have been increasing in colombia in recent years, but its epidemiology is poorly known. we have morphologically and molecularly characterized 89 isolates of fusarium obtained between 2010 and 2012 in the cities of bogotá and medellín. using a multi-locus sequence analysis of rdna internal transcribed spacer, a fragment of the translation elongation factor 1-alpha (tef-1α) and of the rna-dependent polymerase subunit ii (rpb2) genes, we identified the phylogenetic species and circulatin ...201626943726
detection of human pathogenic fusarium species in hospital and communal sink biofilms by using a highly specific monoclonal antibody.the fungus fusarium is well known as a plant pathogen, but has recently emerged as an opportunistic pathogen of humans. habitats providing direct human exposure to infectious propagules are largely unknown, but there is growing evidence that plumbing systems are sources of human pathogenic strains in the fusarium solani species complex (fssc) and fusarium oxysporum species complex (fosc), the most common groups infecting humans. here, a newly developed fusarium-specific monoclonal antibody (mab ...201626914362
rapid identification of moulds and arthroconidial yeasts from positive blood cultures by maldi-tof mass spectrometry.moulds and arthroconidial yeasts are potential life-threatening agents of fungemia in immunocompromised patients. fast and accurate identification (id) of these pathogens hastens initiation of targeted antifungal therapy, thereby improving the patients' prognosis. we describe a new strategy that enabled the identification of moulds and arthroconidial yeasts directly from positive blood cultures by maldi-tof mass spectrometry (ms). positive blood cultures (bcs) with gram staining showing hyphae a ...201627317582
a case of cutaneous fusariosis of the scrotum as a complication of acute myeloid leukemia.fusarium, a hyphomyocetous fungus, is often isolated from the environment as a laboratory contaminant, but is also known as a pathogen causing keratomycosis, onychomycosis, and opportunistic infection of the skin and viscera. we report a 67-year-old man with localized cutaneous fusariosis of the scrotum, as a complication of acute myeloid leukemia (aml) under chemotherapy. an induration of 25 mm in diameter, which was covered by necrosis and black crust and with pain upon pressure, was found on ...201627251318
multilocus phylogeny reveals an association of agriculturally important fusarium solani species complex (fssc) 11, and clinically important fssc 5 and fssc 3 + 4 with soybean roots in the north central united states.the fusarium solani species complex (fssc) includes important root pathogens of soybean in the united states, but the evolutionary lineages associated with soybean root rot are unknown. a multilocus phylogeny based on 93 isolates from soybean and pea roots from north dakota and minnesota revealed that root rot was associated with three known phylogenetic species, fssc 3 + 4 (=fusarium falciforme) (3 % of isolates), fssc 5 (60 %), fssc 11 (34 %), and one unknown species, fssc x (2 %). of these sp ...201626671414
molecular phylogenetics and anti-pythium activity of endophytes from rhizomes of wild ginger congener, zingiber zerumbet smith.zingiber zerumbet, a perennial rhizomatous herb exhibits remarkable disease resistance as well as a wide range of pharmacological activities. towards characterizing the endophytic population of z. zerumbet rhizomes, experiments were carried out during two different growing seasons viz., early-june of 2013 and late-july of 2014. a total of 34 endophytes were isolated and categorized into 11 morphologically distinct groups. fungi were observed to predominate bacterial species with colonization fre ...201626867602
effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte fusarium solani.volumetric productivity of camptothecin from the suspension culture of the endophyte fusarium solani was enhanced up to ∼152 fold (from 0.19 μg l(-1) d(-1) to 28.9 μg l(-1) d(-1)) under optimized fermentation conditions including initial ph (6.0), temperature (32 °c) and agitation speed (80 rpm) with (5% (v/v)) ethanol as medium component. among various elicitors and precursors studied, tryptamine (0.5 mm) as precursor and bovine serum albumin (bsa) (0.075 mm) as an elicitor added on day 6 of th ...201626851893
rhizopus arrhizus and fusarium solani concomitant infection in an immunocompromised host.neutropenic patients are at risk of the development of hyalohyphomycosis and mucormycosis. correct identification is essential for the initiation of the specific treatment, but concomitant mold infections are rarely reported. we report one unprecedented case of concomitant mucormycosis and fusariosis in a neutropenic patient with acute myeloid leukemia. the patient developed rhino-orbital infection by rhizopus arrhizus and disseminated infection by fusarium solani. the first culture from a sinus ...201626346377
epitypification of fusisporium (fusarium) solani and its assignment to a common phylogenetic species in the fusarium solani species complex.fusisporium solani was described as the causal agent of a dry rot of potato in germany in the mid 19th century. as fusarium solani, the species became known as a plurivorous plant pathogen, endophyte, decomposer, and opportunistic pathogen of humans and nutritional symbiont of insects. in parallel, it became evident that the morphologically defined species f. solani represents a phylogenetically and biologically complex group of often morphologically cryptic species that has come to be known in ...201627055573
etiology and epidemiological conditions promoting fusarium root rot in sweetpotato.sweetpotato production in the united states is limited by several postharvest diseases, and one of the most common is fusarium root rot. although fusarium solani is believed to be the primary causal agent of disease, numerous other fusarium spp. have been reported to infect sweetpotato. however, the diversity of fusarium spp. infecting sweetpotato in north carolina is unknown. in addition, the lack of labeled and effective fungicides for control of fusarium root rot in sweetpotato creates the ne ...201627050570
fusariosis in a patient with acute myeloid leukemia: a case report and review of the literature.fusarium spp. causes infections mostly in patients with prolonged neutropenia. we describe the case of a disseminated fusarium solani infection in a patient with acute myeloid leukemia which never reached complete remission during its clinical course. the patient had profound neutropenia and developed skin nodules and pneumonia in spite of posaconazole prophylaxis. f. solani was isolated from blood and skin biopsy, being identified from its morphology and by molecular methods. by broth dilution ...201627008433
two new polyhydroxysterols produced by fusarium solani, an endophytic fungus from chloranthus multistachys.a highly antagonistic endophytic fungus, designated strain cl39, was originated from the leaves of chloranthus multistachys collected in wulong of chongqing municipality of china in november 2015. the strain was identified as fusarium solani based on morphological characteristics, 5.8s gene and internal transcribed spacer sequence analysis. two new compounds, 2β, 9α-dihydroxy-5α-methoxyergosta-7, 22-diene (1), 2β, 6β-dihydroxy-5α-methoxyergosta-7, 22-diene (2) have been isolated from the culture ...201627000714
challenges in fusarium, a trans-kingdom pathogen.fusarium species are emerging human pathogens, next to being plant pathogens. problems with fusarium are in their diagnostics and in their difficult treatment, but also in what are actual fusarium species or rather fusarium-like species. in this issue guevara-suarez et al. (mycopathologia. doi: 10.1007/s11046-016-9983-9 , 2016) characterized 89 isolates of fusarium from colombia showing especially lineages within the fusarium solani and oxysporum species complexes to be responsible for onychomyc ...201626966007
roles of triolein and lipolytic protein in the pathogenesis and survival of mycobacterium tuberculosis: a novel therapeutic approach.discovery of novel secreted enzymes and proteins in mycobacterium tuberculosis (m. tuberculosis) are imperative to understanding the pathogenic system for pathogenesis requires attention. till date, the groups of these secreted enzymes are not meaningfully characterized in terms of m. tuberculosis. in this way, cutinase, a small lipolytic protein, exists in both bacteria and fungi as well which have a potential catalytic activity. during our search, we have found a few genes of m. tuberculosis d ...201626679705
heterologous overexpression and biochemical characterization of the (galactophospho)lipase from fusarium solani in pichia pastoris that is expressed in planta.high-level extracellular production of fusarium solani (galactophospho)lipase, named fsl, was achieved using a pichia pastoris x33 expression system. the (galactophospho) lipase encoding gene was cloned into pgapzαa with the saccharomyces cerevisiae α-factor signal sequence by two different ways. the two constructs consist of an additional sequence of a (his)6-tag of the vector fused to the n-terminus of this enzyme (tfsl) while the other expression vector was constructed without any additional ...201626675137
response surface optimization for decaffeination and theophylline production by fusarium solani.coffee processing industries generate caffeine-containing waste that needs to be treated and decaffeinated before being disposed. five fungal isolates obtained on caffeine-containing mineral media were tested for their ability to utilize caffeine at high concentrations. an isolate identified as fusarium solani could utilize caffeine as a sole source of carbon and nitrogen up to 5 g/l and could degrade it to an extent of 30-53 % in 120 h. sucrose that was added as an auxiliary substrate (5 g/l) e ...201626419659
germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants.controlled transgene expression via a promoter is particularly triggered in response to pathogen infiltration. this is significant for eliciting disease-resistant features in crops through genetic engineering. the germins and germin-like proteins (glps) are known to be associated with plant and developmental stages. the 1107-bp oryza sativa root glp2 (osrglp2) gene promoter fused to a β-glucuronidase (gus) reporter gene was transformed into potato plants through an agrobacterium-mediated transfo ...201626277722
keratitis caused by moulds in santa lucía ophthalmology hospital in buenos aires, argentina.mycotic keratitis by moulds (mkm) is an important cause for corneal blindness and usually carries an unfavorable prognosis.201625999088
secretome analysis of the mycoparasitic fungus trichoderma harzianum all 42 cultivated in different media supplemented with fusarium solani cell wall or glucose.trichoderma harzianum is a fungus well known for its potential as a biocontrol agent against many fungal phytopathogens. the aim of this study was to characterize the proteins secreted by t. harzianum all42 when its spores were inoculated and incubated for 48 h in culture media supplemented with glucose (glu) or with cell walls from fusarium solani (fscw), a phytopathogen that causes severe losses in common bean and soy crops in brazil, as well as other crop diseases around the world. trichoderm ...201626631988
fatal cases of bloodstream infection by fusarium solani and review of published literature.fusarium species are ubiquitously present in environment and are well known as human pathogens with high mortality rate in immunocompromised patients. we report here two cases where immunocompromised patients developed fatal bloodstream infections by this organism. isolates were further identified by its1 region sequencing which confirmed them as fusarium solani. antifungal susceptibility testing was done following clsi m38-a2 guidelines to amphotericin b, fluconazole, itraconazole, voriconazole ...201626541869
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