Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
canine leishmaniosis caused by leishmania major and leishmania tropica: comparative findings and serology.infection and clinical disease associated with leishmania major and leishmania tropica, two common agents of human cutaneous leishmaniosis, have rarely been reported in dogs. this study describes dogs infected with these leishmania spp. prevalent in the middle east and north africa, and compares the serological response of dogs infected with leishmania infantum, l. major or l. tropica to whole promastigote antigen enzyme-linked immunosorbent assay (elisa) of each species and to rk39 dipstick.201728285601
first detection of tick-borne pathogens of dogs from malta.the knowledge about the vector-borne infections in domestic dogs has been increasing worldwide. however no studies have been done on hard tick infestation and tick-borne pathogens (tbps) of dogs present in malta. therefore, a total of 99 dogs was selected and inspected between march and july 2013 in 18 urban and 17 rural areas on the islands of malta and gozo. all ticks were removed from the dogs and identified. blood samples were taken and tested for protozoa (babesia spp. and hepatozoon spp.) ...201728143697
canine tick-borne pathogens in cyprus and a unique canine case of multiple co-infections.canine tick-borne pathogens such as ehrlichia canis and hepatozoon canis are widespread in the mediterranean basin but have never been reported or investigated in cyprus. we describe herein the presence of canine tick-borne pathogens in three dogs with clinical signs compatible with vector-borne diseases from paphos area of cyprus. molecular and phylogenetic analysis revealed the presence of e. canis, anaplasma platys, h. canis, babesia vogeli and mycoplasma haemocanis in cyprus. one dog co-infe ...201728089651
first molecular detection of tick-borne pathogens in dogs from jiangxi, china.in this study, blood samples obtained from 162 dogs in jiangxi, china, were employed in molecular screening of canine tick-borne pathogens by pcr and sequencing. babesia spp. gene fragment was detected in 12 (7.41%) dogs. all samples were negative for hepatozoon spp., ehrlichia canis, coxiella spp., borrelia spp., rickettsia spp. and anaplasma platys. species-specific pcr analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by babesia canis vogeli and babesia gibsoni, respe ...201727890889
black-backed jackals (canis mesomelas) are natural hosts of babesia rossi, the virulent causative agent of canine babesiosis in sub-saharan africa.babesia rossi, which is transmitted by haemaphysalis spp. and is highly virulent to domestic dogs, occurs only in sub-saharan africa. since dogs are not native to the region, it has been postulated that the natural host of b. rossi is an indigenous african canid. although various attempts at artificial infection indicated that black-backed jackals (canis mesomelas) could become subclinically infected with b. rossi, data on occurrence of b. rossi in free-ranging jackals was lacking. a long-term b ...201728285591
is the free-ranging jaguar (panthera onca) a reservoir for cytauxzoon felis in brazil?this study investigated the occurrence of cytauxzoon felis and babesia spp. in free-ranging jaguars (panthera onca), domestic dogs (canis lupus familiaris) and domestic cats (felis catus) from the cerrado, amazon and pantanal biomes of brazil. blood samples were collected from 30 jaguars, 129 dogs and 22 cats for detection of the 18s rrna genes of piroplasmids. all of the jaguars from the pantanal (n=22) and cerrado (n=4) and three of four jaguars from the amazon were positive for c. felis, but ...201728196774
detection and molecular identification of hepatozoon canis and babesia vogeli from domestic dogs in palestine.dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. this study aimed to observe tick borne pathogens in dogs from palestine including hepatozoon canis and babesia species. the prevalence of both h. canis and babesia species infections in apparently healthy dogs, from ten districts of the west bank was surveyed. dna was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). a primer set that amplifi ...201727938422
acute phase proteins response in cats naturally infected with hepatozoon felis and babesia vogeli.the measurement of acute phase proteins (app) is being increasingly used in human and veterinary medicine in diagnosis, prognosis, treatment monitoring, and in general health screening. however, information about the app response in cats infected with agents of vector-borne diseases is lacking.201728160310
a new pcr assay for the detection and differentiation of babesia canis and babesia vogeli.babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. diagnosis of babesiosis is often impeded by the transient presence of the parasites in peripheral blood, as well as by their pleomorphic nature. given the reports of an expanding and, in some cases, sympatric geographical distribution of babesia canis and babesia vogeli in dogs and associated vectors, in europe, the development of time-effici ...201728739301
a pcr survey of vector-borne pathogens in different dog populations from turkey.in the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in konya district, turkey, and tested by specific pcr for the presence of vector-borne pathogens. several pathogens were identified, most of which can cause substantial morbidity in dogs. pcr results revealed that 54 (28.1%) dogs were infected with one or more pathogens. positive results were obtained for babesia spp. in 4 dogs (2.1%), hepatozoon spp. in 8 dogs (4.2%) and mycoplasma spp ...201728682765
a survey of canine haemoprotozoan parasites from turkey, including molecular evidence of an unnamed babesia.canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. a reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as hepatozoon canis. ten samples hybridized to the theileria/babesia and babesia catch all probes but did not hybridize to any species-specific probe tested, suggesting the presence of an unrec ...201728673460
first molecular detection of piroplasm infection in pet dogs from gansu, china.babesiosis, the hemolytic disease caused by babesia, which is a tick-transmitted obligate intraerythrocytic protozoan parasite. this disease is responsible for significant mortality and morbidity rates and enormous economic losses to the livestock industry in tropical and subtropical regions worldwide. in this study, blood samples were collected from 141 pet dogs from gansu, china, and analyzed for babesia or theileria spp. infection using specific pcr and sequencing based on 18s rrna gene fragm ...201728638376
molecular detection of vector-borne pathogens in dogs and cats from qatar.vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries.201728633666
molecular identification of babesia spp. in naturally infected dogs of kerala, south india.canine babesiosis is a tick borne haemoprotozoan disease caused by large and small intraerythrocytic apicomplexan piroplasms of the genus babesia spp. the clinical manifestations of the disease vary from inapparent subclinical form to hyperacute shock related haemolytic crisis. microscopic examination of blood smears from suspected dogs revealed babesia canis and b. gibsoni in 3.45 and 25.86 % of samples respectively. a seminested pcr based on previously published species specific primers target ...201728615859
first report on babesia vogeli infection in dogs in the philippines.babesia vogeli is a tick-borne protozoal pathogen that infects erythrocytes. in southeast asia, this pathogen has only been reported in thailand. in this study, nine dogs presented at three different veterinary clinics in cebu city, philippines were found positive for b. vogeli. dna was extracted from blood samples and tested using a pcr for genus babesia and a pcr specific for b. vogeli (both based on the 18s rrna gene). blood smears (triplicate) from each sample were found negative. all positi ...201727713098
vector-borne and zoonotic diseases of dogs in north-west new south wales and the northern territory, australia.vector-borne diseases of dogs in australian aboriginal communities are relatively unexplored. these dogs represent a unique group with variable ecto- and endo-parasitic burdens, nutritional stresses and a general lack of veterinary intervention. we investigated haemoprotozoal and bacterial pathogen prevalences in relation to erythrocyte and platelet numbers in dogs from north-west new south wales (n-w nsw) and the northern territory (nt; central australia).201728810870
an elisa for the early diagnosis of acute canine babesiosis detecting circulating antigen of large babesia spp.babesia canis is the predominant babesia species in dogs in europe and is responsible for a severe and fatal disease. an increase in global pet tourism and a widening of the geographic distribution of the tick vector has led to the emergence of infections in areas where previously only imported cases have been reported. due to the potential for rapid and serious disease progression, direct parasite detection by stained blood smears and light microscopy or dna-based methods have traditionally bee ...201728807287
molecular detection of vector-borne pathogens in wild and domestic carnivores and their ticks at the human-wildlife interface.urbanization of natural areas is considered one of the causes of the current apparent emergence of infectious diseases. carnivores are among the species that adapt well to urban and periurban environments, facilitating cross-species disease transmission with domestic dogs and cats, and potentially with their owners. the prevalence of vector-borne pathogens (vbp) of zoonotic and veterinary interest was studied in sympatric wild and domestic carnivores into barcelona metropolitan area (ne spain). ...201626643497
first detection and molecular identification of babesia vogeli from rhipicephalus sanguineus (acari: ixodidae) in taiwan.a total of 578 rhipicephalus sanguineus ticks collected from dogs in taiwan were examined for babesia by species-specific polymerase chain reaction assay based on the 18s small subunit ribosomal rna (ssrrna) gene. babesia dna was detected in 1.04 % (6/578) of rh. sanguineus ticks. phylogenetic analysis revealed that these babesia spp. were genetically linked to the same clade within the genospecies of babesia vogeli and could be discriminated from other genospecies of babesia. intra-species anal ...201626796569
molecular prevalence and genetic characterization of piroplasms in dogs from tunisia.in this study, the prevalence of piroplasms in dogs was assessed using polymerase chain reaction (pcr) to identify babesia and theileria species in 200 dogs from northern and central tunisia between spring and autumn 2014. the overall molecular prevalence for piroplasms was 14·5% ± 0·05 (29/200); pcr detected 2 species, namely babesia vogeli and theileria annulata with an overall prevalence of 12·5 ± 0·04 and 2% ± 0·02, respectively. no differences in the molecular prevalences of b. vogeli were ...201627417681
molecular analysis of rhipicephalus sanguineus (acari: ixodidae), an incriminated vector tick for babesia vogeli in taiwan.the genetic identity of rhipicephalus sanguineus tick was determined for the first time in taiwan. the phylogenetic relationships were analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 32 strains of ticks representing six species of rhipicephalus, two species of dermacentor and two outgroup species (haemaphysalis inermis and ixodes ricinus). seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony ...201627815655
rangelia vitalii, babesia spp. and ehrlichia spp. in dogs in passo fundo, state of rio grande do sul, brazil.pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents babesia vogeli and ehrlichia canis and conventional pcr for babesia spp. (gene 18s rr ...201627334817
rangelia vitalii, babesia spp. and ehrlichia spp. in dogs in passo fundo, state of rio grande do sul, brazil.pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents babesia vogeli and ehrlichia canis and conventional pcr for babesia spp. (gene 18s rr ...201627304518
identification of vector-borne pathogens in dogs and cats from southern brazil.dogs and cats are often infected with vector-borne pathogens and play a crucial role as reservoirs and hosts in their life cycles. the aim of the present study was to investigate the occurrence of vector-borne pathogens among dogs and cats in the northwestern region of rio grande do sul (rs) state, brazil. one hundred and ten blood samples were collected from dogs (n=80) and cats (n=30). laboratory analysis were carried out through stained blood smears, indirect enzyme-linked immunosorbent assay ...201627266811
mitochondrial genome sequences and structures aid in the resolution of piroplasmida phylogeny.the taxonomy of the order piroplasmida, which includes a number of clinically and economically relevant organisms, is a hotly debated topic amongst parasitologists. three genera (babesia, theileria, and cytauxzoon) are recognized based on parasite life cycle characteristics, but molecular phylogenetic analyses of 18s sequences have suggested the presence of five or more distinct piroplasmida lineages. despite these important advancements, a few studies have been unable to define the taxonomic re ...201627832128
withdrawn:first report on babesia canis vogeli infection in dogs in the philippines.the publisher regrets that this article is an accidental duplication of an article that has already been published, http://dx.doi.org/10.1016/j.parint.2016.10.001.the duplicate article has therefore been withdrawn. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy.201627815229
confirmation of occurrence of <i>babesia vogeli</i> in a dog in windhoek, central namibia.although there is evidence of high seroprevalence of antibodies to babesia spp. in dogs in central namibia, clinical babesiosis is rarely diagnosed. rhipicephalus sanguineus sensu lato, the vector of babesia vogeli, is common in namibia while haemaphysalis elliptica, the vector of the highly virulent but morphologically indistinguishable babesia rossi, has rarely been recorded, mainly in northern namibia. on the basis of vector occurrence, clinical cases of canine babesiosis in windhoek, central ...201627796110
development of recombinant bgp12 based enzyme linked immunosorbent assays for serodiagnosis of babesia gibsoni infection in dogs.indirect elisa and dot-elisa using recombinant bgp12 (rbgp12) were developed for the diagnosis of babesia gibsoni infected dogs. the complete open reading frame of bgp12 gene (378bp) was cloned in pet-32a(+) expression vector and expressed in escherichia coli as a soluble thioredoxin (trx) fusion protein. the purified rbgp12 was used for production of anti-rbgp12 rabbit serum, which recognized a native 12-kda protein in b. gibsoni infected erythrocyte by western blot analysis. to evaluate the po ...201626827835
critical analysis of vector-borne infections in dogs: babesia vogeli, babesia gibsoni, ehrlichia canis and hepatozoon canis in punjab, india.there are few published studies on various vector borne diseases of dogs in india and most depict clinical infection in dogs, diagnosed by observation of the haemopathogens in stained blood smears. this study provides the first report regarding molecular confirmation and ancestral relationship analysis of blood smears positive cases of assorted haemopathogens in punjab province of india. on blood smear examination, haemopathogens were observed in 124 out of 778 (15.95%, 95% ci: 13.53- 18.68) blo ...201627787221
molecular detection of theileria, babesia, and hepatozoon spp. in ixodid ticks from palestine.ixodid ticks transmit various infectious agents that cause disease in humans and livestock worldwide. a cross-sectional survey on the presence of protozoan pathogens in ticks was carried out to assess the impact of tick-borne protozoa on domestic animals in palestine. ticks were collected from herds with sheep, goats and dogs in different geographic districts and their species were determined using morphological keys. the presence of piroplasms and hepatozoon spp. was determined by pcr amplifica ...201626969489
clinical and laboratory features of canine anaplasma platys infection in 32 naturally infected dogs in the mediterranean basin.since the first description of anaplasma platys infection (api), the disease has been sporadically reported worldwide. whereas it is considered a subclinical disease in the united states or in australia, severe cases are reported in europe. thus far, little information is available regarding the clinical and laboratory findings associated with the disease and the implication of co-infections with other vector-borne pathogens (vbps) in southern europe. the purpose of the study was to describe cli ...201627426437
detection of ehrlichia canis, babesia vogeli, and toxoplasma gondii dna in the brain of dogs naturally infected with leishmania infantum.the aims of this study were to investigate the presence of leishmania infantum and possible co-infection with anaplasma platys , babesia vogeli, ehrlichia canis , and toxoplasma gondii in the brain of 24 dogs naturally infected by l. infantum . a total of 24 mongrel adult dogs (22 clinically affected, 2 with neurological signs, and 2 subclinically infected) aged between 2 and 5 yr, naturally infected by visceral leishmaniasis, were selected. fragments from meninges, frontal cortex, thalamus, cer ...201626765523
a survey of tick-borne pathogens in dogs and their ticks in the pantanal biome, brazil.tick and blood samples collected from domestic dogs in the brazilian pantanal were tested by molecular methods for the presence of tick-borne protozoa and bacteria. among 320 sampled dogs, 3.13% were infected by babesia vogeli (piroplasmida: babesiidae), 8.75% by hepatozoon canis (eucoccidiorida: hepatozoidae), 7.19% by anaplasma platys (rickettsiales: anaplasmataceae), and 0.94% by an unclassified anaplasma sp. in three tick species collected from dogs, the following tick-borne agents were dete ...201626467462
detection of mycoplasma haemocanis, mycoplasma haematoparvum, mycoplasma suis and other vector-borne pathogens in dogs from córdoba and santa fé, argentina.in argentina, only very few reports are available for canine tick-borne diseases where most are related to parasitic diseases. the objective of this survey was to investigate the prevalence of tick-borne pathogens in 70 dogs from santa fé and córdoba, argentina.201627978844
prevalence of vector-borne pathogens in dogs from haiti.canine vector-borne pathogens are common on some caribbean islands, but survey data in haiti are lacking. to determine the prevalence of selected vector-borne pathogens in dogs from haiti, we tested blood samples collected from 210 owned dogs, 28 (13.3%) of which were infested with rhipicephalus sanguineus ticks at the time of blood collection. no other tick species were identified on these dogs. a commercially available elisa identified antibodies to ehrlichia spp. in 69 (32.9%), antibodies to ...201627270383
parasites and vector-borne pathogens in client-owned dogs in albania. blood pathogens and seroprevalences of parasitic and other infectious agents.knowledge on the epidemiology of parasitic and vector-borne infections is still very limited for albania, a country located in the balkan peninsula in southeast europe. recent publications indicated prevalence rates of up to 52% for vector-borne infections in less-cared dogs in albania. to provide data on the epidemiological situation in dogs under veterinary care, a total of 602 client-owned dogs presented to four small animal clinics between march 2010 and april 2011 in tirana, albania, were s ...201626453093
detection of vector-borne pathogens in cats and their ectoparasites in southern italy.vector-borne pathogens are the subject of several investigations due to the zoonotic concern of some of them. however, limited data are available about the simultaneous presence of these pathogens in cats and their ectoparasites. the aim of the present study was to define the species of ectoparasites found on cats as well as to investigate vector-borne pathogens in cats and their ectoparasites in southern italy.201627160725
molecular investigation of tick-borne pathogens in dogs from luanda, angola.no molecular data have been available on tick-borne pathogens that infect dogs from angola. the occurrence of agents from the genera anaplasma, babesia, ehrlichia and hepatozoon was assessed in 103 domestic dogs from luanda, by means of the polymerase chain reaction (pcr) and dna sequence analysis.201627160839
canine vector-borne pathogens in semi-domesticated dogs residing in northern cambodia.in southeast asia, the canine vector-borne pathogens babesia spp., ehrlichia canis, anaplasma platys, hepatozoon canis, haemotropic mycoplasmas and dirofilaria immitis cause significant morbidity and mortality in dogs. moreover, dogs have also been implicated as natural reservoirs for rickettsia felis, the agent of flea-borne spotted fever, increasingly implicated as a cause of undifferentiated fever in humans in southeast asia. the objective of this study was to determine the prevalence and div ...201627161452
comparative speed of kill of sarolaner (simparica) and afoxolaner (nexgard) against induced infestations of rhipicephalus sanguineus s.l. on dogs.the brown dog tick, rhipicephalus sanguineus sensu lato, commonly infests dogs globally, is the major vector of the pathogen that causes canine monocytic ehrlichiosis and also transmits babesia vogeli. a rapid speed of kill of a parasiticide is essential to reduce the direct deleterious effects of tick infestation and the risk of tick-borne pathogen transmission. the speed of kill of a novel orally administered isoxazoline parasiticide, sarolaner (simparica), against r. sanguineus sensu lato on ...201626896456
acute febrile illness is associated with rickettsia spp infection in dogs.rickettsia conorii is transmitted by rhipicephalus sanguineus ticks and causes mediterranean spotted fever (msf) in humans. although dogs are considered the natural host of the vector, the clinical and epidemiological significance of r. conorii infection in dogs remains unclear. the aim of this prospective study was to investigate whether rickettsia infection causes febrile illness in dogs living in areas endemic for human msf.201525886403
detection of dirofilaria immitis and other arthropod-borne filarioids by an hrm real-time qpcr, blood-concentrating techniques and a serological assay in dogs from costa rica.canine filarioids are important nematodes transmitted to dogs by arthropods. diagnosis of canine filariosis is accomplished by the microscopic identification of microfilariae, serology or pcr for filarial-dna. the aim of this study was to evaluate a molecular assay for the detection of canine filariae in dog blood, to compare its performance to other diagnostic techniques, and to determine the relationship between microfilarial concentration and infection with other vector-borne pathogens.201525851920
ticks and associated pathogens collected from cats in sicily and calabria (italy).limited information is available about the species of ticks infesting the cat and the pathogens that they harbor. the aims of the present study were to identify the species of ticks removed from cats living in sicily and calabria (italy) and to detect dna of vector-borne pathogens in the same ticks.201526445916
molecular detection of vector-borne agents in dogs from ten provinces of china.although many vector-borne agents are potential zoonoses and cause substantial morbidity and mortality in dogs worldwide, there are limited data on these organisms in dogs of china.201526428085
tick-borne diseases (borreliosis, anaplasmosis, babesiosis) in german and austrian dogs: status quo and review of distribution, transmission, clinical findings, diagnostics and prophylaxis.tick-borne diseases (tbd) in dogs have gained in significance in german and austrian veterinary practices. the widespread european tick species ixodes ricinus represents an important vector for spirochaetes of the borrelia burgdorferi sensu lato group and rickettsiales such as anaplasma phagocytophilum. the meadow or ornate dog tick (dermacentor reticulatus) is an important vector for babesia canis, as is the brown dog tick (rhipicephalus sanguineus) for babesia vogeli in the mediterranean regio ...201526152408
spatial distribution of anaplasma phagocytophilum and hepatozoon canis in red foxes (vulpes vulpes) in hungary.in recent years, ehrlichia canis and hepatozoon canis transmitted by rhipicephalus sanguineus were reported from hungary. the aim of the present study was to reveal the spatial distribution pattern of pathogens transmitted by r. sanguineus in a sentinel species, red fox (vulpes vulpes) in hungary and to analyse the relationship of these patterns with landscape and climate by geographical information systems. fox carcasses, representing 0.5% of the total fox population were randomly selected out ...201526065623
tick-borne agents in domesticated and stray cats from the city of campo grande, state of mato grosso do sul, midwestern brazil.anaplasmataceae agents, piroplasmids and hepatozoon spp. have emerged as important pathogens among domestic and wild felines. the present work aimed to detect the presence of species belonging to the anaplasmataceae family, piroplasmas and hepatozoon spp. dna in blood samples of domesticated and stray cats in the city of campo grande, state of mato grosso do sul, midwestern brazil. between january and april 2013, whole blood samples were collected from 151 cats (54 males, 95 females and two with ...201526187416
vector-borne diseases of small companion animals in namibia: literature review, knowledge gaps and opportunity for a one health approach.namibia has a rich history in veterinary health but little is known about the vector-borne diseases that affect companion dogs and cats. the aim of this review is to summarise the existing published and available unpublished literature, put it into a wider geographical context, and explore some significant knowledge gaps. to date, only two filarial pathogens (dirofilaria repens and acanthocheilonema dracunculoides) and three tick-borne pathogens (babesia canis vogeli, hepatozoon canis and ehrlic ...201526824340
molecular detection of ehrlichia canis, hepatozoon canis and babesia canis vogeli in stray dogs in mahasarakham province, thailand.canine tick borne diseases showing distribution worldwide have caused morbidity and mortality in dogs. this study observed the mainly tick borne pathogens described for dogs in thailand, ehrlichia canis, hepatozoon canis and babesia canis vogeli. from may to july 2014, blood samples were collected from 79 stray dogs from 7 districts of mahasarakham province to molecular surveyed for 16s rrna gene of e. canis and 18s rrna gene of h. canis and b. canis vogeli. twenty eight (35.44%) of stray dogs s ...201526568991
leishmania, babesia and ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?the present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-leishmania spp., -babesia canis vogeli and -ehrlichia canis antibodies in urban dogs from an area endemic to these parasites.201525860466
detection of babesia canis vogeli and hepatozoon canis in canine blood by a single-tube real-time fluorescence resonance energy transfer polymerase chain reaction assay and melting curve analysis.a real-time fluorescence resonance energy transfer polymerase chain reaction (qfret pcr) coupled with melting curve analysis was developed for detection of babesia canis vogeli and hepatozoon canis infections in canine blood samples in a single tube assay. the target of the assay was a region within the 18s ribosomal rna gene amplified in either species by a single pair of primers. following amplification from the dna of infected dog blood, a fluorescence melting curve analysis was done. the 2 s ...201525776544
development of loop-mediated isothermal amplification (lamp) for detection of babesia gibsoni infection in dogs.diagnosis of canine babesiosis, caused by babesia gibsoni is difficult, especially in chronically infected dogs. a loop mediated isothermal amplification (lamp) assay was developed and standardized by using four oligonucleotide primers targeting the hypervariable region of 18s rrna gene (genbank acc. no. kc461261). the primers specifically amplified b. gibsoni dna, while no amplification was detected with dna from non-infected dogs as well as from dogs infected with babesia canis vogeli, hepatoz ...201525749021
seroprevalence of tick-borne pathogens and tick infestation in dogs from tapirapé and karajá indigenous communities, brazil.with the aim of studying some tick-borne diseases, a total of 327 dogs (114 from tapirapé and 213 from karajá indigenous ethnicity, mato grosso, mt, brazil) were sampled. serum samples were submitted to the indirect fluorescence antibody test (ifat) to detect antibodies against babesia vogeli, ehrlichia canis, and rickettsia spp. possible associations of risk factors and the occurrence of seroreativity to tick-borne agents and tick infestations were analyzed through chi-squared tests. among 327 ...201526186512
survey of ehrlichia canis, babesia spp. and hepatozoon spp. in dogs from a semiarid region of brazil.this study assessed the occurrence of ehrlichia spp., babesia spp. and hepatozoon spp. infections in 100 tick-harboring dogs from a semiarid region of the state of paraíba, northeastern brazil. blood samples and ticks were collected from the animals, and a questionnaire was submitted to dog owners to obtain general data. blood samples were used to perform hemogram, direct blood smear and immunological and molecular hemoparasite detection. the 1,151 ticks collected were identified as rhipicephalu ...201525909253
a serological and molecular survey of babesia vogeli, ehrlichia canis and rickettsia spp. among dogs in the state of maranhão, northeastern brazil.this study evaluated exposure and infection by tick-borne agents (babesia vogeli, ehrlichia canis and rickettsia spp.) in 172 dogs in rural areas and 150 dogs in urban areas of the municipality of chapadinha, state of maranhão, northeastern brazil, using molecular and serological methods. overall, 16.1% of the sampled dogs (52/322) were seroreactive to b. vogeli, with endpoint titers ranging from 40 to 640. for e. canis, 14.6% of the dogs (47/322) were seroreactive, with endpoint titers from 80 ...201525909250
molecular characterization of babesia vogeli in dogs from belém, northern brazil.babesiosis is an infectious hemolytic disease that occurs worldwide, and is caused by a protozoan of the babesia genus (apicomplexa). little is known about this genus; therefore, this study conducted a molecular characterization of babesia spp in naturally infected dogs in northern brazil. blood samples were collected from 172 dogs from metropolitan belém, and screened for babesia spp using semi-nested polymerase chain reactions. subsequently, 18s rdna gene fragments were amplified and sequenced ...201526662431
investigation of the 8-hydroxy-2'-deoxyguanosine, total antioxidant and nitric oxide levels of serum in dogs infected with babesia vogeli.oxidative stress was defined as corruption of balance between oxidant-antioxidant states in favor of oxidants. in this study, it was aimed to determine oxidative stress in naturally infected dogs with babesia vogeli. the 8-hydroxy-2'-deoxyguanosine (8-ohdg), nitric oxide (no) and total antioxidant capacity (tac) levels in the sera were analyzed by elisa. 8-ohdg levels increased in b. vogeli infected dogs compared to control group (p<0.05). also, no levels increased while toc levels decreased in ...201424882747
development and evaluation of serodiagnostic assays with recombinant bgsa1 of babesia gibsoni.indirect elisa, dot-elisa and double antibody sandwich elisa (das-elisa) using truncated recombinant bgsa1 (rbgsa1) were developed for detecting babesia gibsoni infection in naturally infected dogs. truncated bgsa1 gene of 858 bp, encoding 32 kda protein was cloned in pet-32a(+) expression vector, expressed in escherichia coli and the recombinant protein was purified under native conditions. to evaluate the ability of the truncated rbgsa1as serodiagnostic reagent for b. gibsoni infection, a pane ...201425241636
optimization of a molecular method for the diagnosis of canine babesiosis.babesiosis is a hemolytic disease caused by protozoans of the genus babesia (apicomplexa). this disease occurs worldwide and is transmitted by ticks to a variety of mammals, including humans. the objective of the present study was to optimize a molecular approach for the detection of a fragment of 18s rdna of babesia canis, babesia vogeli, babesia rossi or babesia gibsoni based on a single semi-nested polymerase chain reaction (pcr), and compare the efficiency of this approach with that of a sim ...201424728371
bacterial and protozoal agents of feline vector-borne diseases in domestic and stray cats from southern portugal.feline vector-borne diseases (fvbd) have emerged in recent years, showing a wider geographic distribution and increased global prevalence. in addition to their veterinary importance, domestic cats play a central role in the transmission cycles of some fvbd agents by acting as reservoirs and sentinels, a circumstance that requires a one health approach. the aim of the present work was to molecularly detect feline vector-borne bacteria and protozoa with veterinary and zoonotic importance, and to a ...201424655431
clinical, hematologic, and molecular findings in naturally occurring babesia canis vogeli in egyptian dogs.background. canine babesiosis is a clinically important hemoprotozoan parasite affecting dogs. the goal of this present study was to determine the clinical symptoms and to establish its hematological and microscopic detection and compare it with the pcr findings attained from dogs infected with babesia canis vogeli. methodology/principal findings. 13-pcr confirmed babesia-infected dogs were examined; seminested pcr was used to discover the precise type of babesia and babesia canis vogeli was the ...201424693460
incidence of cercopithifilaria bainae in dogs and probability of co-infection with other tick-borne pathogens.cercopithifilaria bainae is a filarioid parasite that infects dogs, being transmitted by rhipicephalus sanguineus group ticks in many countries of the mediterranean basin. this study assessed the incidence density rate (idr) of infection by c. bainae in dogs and the probability of co-infection with other tick-borne pathogens (i.e., anaplasma platys, babesia vogeli and hepatozoon canis), in an area of high endemicity in southern italy.201424498437
arthropod-borne pathogens circulating in free-roaming domestic cats in a zoo environment in brazil.recently, tick and flea-borne pathogens have been detected in wild carnivores maintained in captivity in brazilian zoos. since free-roaming cats are frequently found in brazilian zoos, they could act as reservoirs for arthropod-borne pathogens, which could be transmitted to endangered wild carnivores maintained in captivity in these institutions. on the other hand, stray cats in zoos may play a role as sentinels to pathogens that circulate among wild animals in captivity. the present work aimed ...201424889035
molecular detection of bacterial and parasitic pathogens in hard ticks from portugal.ticks are important vector arthropods of human and animal pathogens. as information about agents of disease circulating in vectors in portugal is limited, the aim of the present study was to detect bacteria and parasites with veterinary and zoonotic importance in ticks collected from dogs, cats, and field vegetation. a total of 925 ticks, comprising 888 (96.0%) adults, 8 (0.9%) nymphs, and 29 (3.1%) larvae, were collected in 4 geographic areas (districts) of portugal. among those, 620 (67.0%) we ...201424745731
evaluation of blood and bone marrow in selected canine vector-borne diseases.bone marrow (bm) is a major hematopoietic organ that can harbour a variety of vector-borne pathogens; however, knowledge of bm pathological changes in dogs infected with vector-borne pathogens is limited. thus, the aim of the present study was to assess the pathological changes in canine bm associated with natural infections by four vector-borne pathogens, as well as to determine the relationships between such changes and abnormalities of the peripheral blood.201425441458
high throughput pyrosequencing technology for molecular differential detection of babesia vogeli, hepatozoon canis, ehrlichia canis and anaplasma platys in canine blood samples.canine babesiosis, hepatozoonosis, ehrlichiosis, and anaplasmosis are tick-borne diseases caused by different hemopathogens. these diseases are causes of morbidity and mortality in dogs. the classic method for parasite detection and differentiation is based on microscopic observation of blood smears. the limitations of the microscopic method are that its performance requires a specially qualified person with professional competence, and it is ineffective in differentiating closely related specie ...201424704311
molecular and serological detection of tick-borne pathogens in dogs from an area endemic for leishmania infantum in mato grosso do sul, brazil.tick-borne pathogens affect a wide range of vertebrate hosts. to identify tick-borne pathogens among dogs from campo grande, ms, brazil testing seropositive for leishmania infantum (syn. l. chagasi), a serological and molecular study was conducted to detect ehrlichia canis, anaplasma platys and babesia vogeli in 60 serum and spleen samples. a confirmatory diagnosis of l. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to ...201424473877
vector-borne pathogens in dogs from costa rica: first molecular description of babesia vogeli and hepatozoon canis infections with a high prevalence of monocytic ehrlichiosis and the manifestations of co-infection.infection with canine vector-borne pathogens was evaluated in dogs from four different regions of costa rica by pcr. demographic data, clinical signs, packed cell volume values, and the presence of tick infestation were recorded for each dog. forty seven percent (69/146) of the dogs were infected with at least one pathogen and 12% were co-infected with two pathogens. ehrlichia canis was detected in 34%, anaplasma platys in 10%, babesia vogeli in 8%, and hepatozoon canis in 7.5% of the blood samp ...201424315693
babesia canis vogeli, ehrlichia canis, and anaplasma platys infection in a dog.a 12-month-old male neutered mixed breed dog was presented with a history of diarrhea, lethargy, emaciation, polydypsia, and sniffling. physical examination findings included pale mucous membranes, increased heart and respiratory rates, and normal rectal temperature (38°c). hematologic abnormalities included anemia and thrombocytopenia. biochemical abnormalities included hypoalbuminemia, hyperbilirubinemia, and elevated alp and alt activities. a snap 4dx test result was positive for ehrlichia ca ...201324138512
tick-borne pathogens and disease in dogs on st. kitts, west indies.between 2009 and 2011, we conducted a case-control study of ticks and tick-associated pathogens affecting dogs on the island of st. kitts, eastern caribbean, including 55 cases of clinically suspected tick-borne disease (tbd) and 110 presumably healthy animals presented for elective surgeries. rhipicephalus sanguineus caused year-round infestations of dogs, and 36% of the dogs in the study were infested at the time of examination. overall, 62% of suspected tbd cases and 24% of presumably healthy ...201323481028
serosurvey of tick-borne pathogens in dogs from urban and rural areas from parana state, brazil.considering the zoonotic potential of tick-borne disease (tbd) agents and the fact that dogs may act as sentinels for human infection, the aim of the present study was to determine the seroprevalence of tbd agents and risk factors for exposure in two different canine populations from parana state, southern brazil. a total of 138 dog serum samples from urban (ua) (n=68) and rural (ra) (n=70) areas were tested with commercial elisa rapid test for anaplasma phagocytophilum, ehrlichia canis and borr ...201324252955
efficacy of an imidacloprid/flumethrin collar against fleas, ticks and tick-borne pathogens in dogs.tick-borne diseases comprise a group of maladies that are of substantial medical and veterinary significance. a range of tick-borne pathogens, including diverse species of bacteria and protozoa, can infect both dogs and humans. hence, the control of tick infestations is pivotal to decrease or prevent tick-borne pathogen transmission. therefore, different commercial products with insecticidal, repellent or both properties have been developed for use on dogs. recently, a collar containing a combin ...201323972013
molecular detection and characterization of tick-borne pathogens in dogs and ticks from nigeria.only limited information is currently available on the prevalence of vector borne and zoonotic pathogens in dogs and ticks in nigeria. the aim of this study was to use molecular techniques to detect and characterize vector borne pathogens in dogs and ticks from nigeria.201323505591
feline vector-borne pathogens in the north and centre of portugal.in recent years, several clinical cases and epidemiological studies of feline vector-borne diseases (fvbd) have been reported worldwide. nonetheless, information on fvbd agents and their prevalence in portugal is scarce.201323587366
mortality in virulent canine babesiosis is associated with a consumptive coagulopathy.the inflammatory response to infection can activate the coagulation system via complex interactions. if uncontrolled, this may lead to a consumptive coagulopathy, a major risk factor for a poor clinical outcome. this prospective observational study was conducted to determine whether consumptive coagulopathy in dogs with babesia rossi infection is related to mortality. seventy-two client-owned dogs diagnosed with canine babesiosis were included. diagnosis was confirmed by polymerase chain reactio ...201323098634
serosurvey for tick-borne diseases in dogs from the eastern amazon, brazil.canine ehrlichiosis and babesiosis are the most prevalent tick-borne diseases in brazilian dogs. few studies have focused attention in surveying tick-borne diseases in the brazilian amazon region. a total of 129 blood samples were collected from dogs living in the brazilian eastern amazon. seventy-two samples from dogs from rural areas of 19 municipalities and 57 samples from urban stray dogs from santarém municipality were collected. serum samples were submitted to indirect immunofluorescence a ...201323802236
identification of babesia species infecting dogs using reverse line blot hybridization for six canine piroplasms, and evaluation of co-infection by other vector-borne pathogens.canine infection by vector-borne hemoparasites is frequent in tropical and sub-tropical areas where exposure to hematophageous ectoparasites is intensive. a reverse line blot (rlb) assay was designed to improve the simultaneous detection of all named canine piroplasm species combined with other vector-borne pathogens of dogs including ehrlichia canis, hepatozoon canis and leishmania infantum common in the mediterranean basin. blood samples of 110 dogs from spain (n=21), portugal (n=14) and israe ...201323017370
babesia vogeli in a quarantined dog. 201323457280
detection and molecular characterization of piroplasms species from naturally infected dogs in southeast brazil.rangelia vitalii is a protozoon described from dogs in the south and southeast regions of brazil. it is phylogenetically related to babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. the aim of this work was to detect piroplasm species in dogs in the state of rio de janeiro, brazil, by 18s rrna gene-based pcr assay, restriction fragment length polymorphism (rflp) and sequence analyses. of 103 dogs examined, seven (6.8%) were positive for babesia spp. by pcr. th ...201322832754
apparent tick paralysis by rhipicephalus sanguineus (acari: ixodidae) in dogs.certain tick species including ixodes holocyclus can inoculate neurotoxins that induce a rapid, ascending flaccid paralysis in animals. rhipicephalus sanguineus, the most widespread tick of dogs, is recognized as a vector of several pathogens causing diseases in dogs and humans. a single report suggests its role as cause of paralysis in dogs. this study presents the clinical history of 14 young dogs heavily infested by r. sanguineus (intensity of infestation, 63-328) in an endemic area of southe ...201222546547
first evidence and molecular characterization of babesia vogeli in naturally infected dogs and rhipicephalus sanguineus ticks in southern france.babesiosis is an emerging tick-borne disease of animals and humans caused by intraerythrocytic protozoa of the genera babesia and theileria. in france canine babesiosis has a high prevalence with babesia canis thought to be the main aetiological agent of the disease. babesia vogeli has already been reported to occur in europe and in other countries around the mediterranean sea. the tick rhipicephalus sanguineus, the main known vector of b. vogeli, occurs in southern france. however, only one cas ...201222342131
first description of natural ehrlichia canis and anaplasma platys infections in dogs from argentina.bacteria belonging to the anaplasmataceae family are vector transmitted agents that affect a variety of vertebrate hosts including the tick-borne pathogens ehrlichia canis and anaplasma platys, which cause canine monocytic ehrlichiosis and cyclic thrombocytopenia, respectively. these two infections, typically reported from tropical and sub-tropical regions, have not been previously reported in dogs from argentina. a total of 86 blood samples from dogs with suspected rickettsial disease and 28 no ...201223273677
[investigation on babesia in ticks infested on police dogs in selected areas of china].399 tick specimens were collected from the body surface of police dogs in chongqing municipality, provinces of fujian, guangdong, hainan, guangxi, hebei, henan, shanxi, jiangsu and zhejiang. nested-pcr and sequence testing were taken to investigate the prevalence of babesia sp. in ticks. the results showed that babesia vogeli was found in ticks infested on the body surface of police dogs, with a positive rate of 5.3%. the prevalence in chongqing, guangdong, guangxi, hainan and zhejiang was 4/16, ...201223484282
haemoparasites of free-roaming dogs associated with several remote aboriginal communities in australia.tick-borne haemoparasites babesia vogeli and anaplasma platys are common among the free-roaming canine populations associated with aboriginal communities in australia, whilst the prevalence of haemoplasmas, which are also suspected to be tick-borne, remained unexplored. the aim of this study was to determine the prevalence of haemoplasma infection in these populations, and to identify any correlation with other haemoparasites. blood was collected from 39 dogs associated with four aboriginal comm ...201222584120
molecular detection of ehrlichia canis, anaplasma bovis, anaplasma platys, candidatus midichloria mitochondrii and babesia canis vogeli in ticks from israel.: ticks are vectors of important pathogens of human and animals. therefore, their microbial carriage capacity is constantly being investigated. the aim of this study was to characterize the diversity of domestic animal pathogens in ticks collected from vegetation and the ground, from different parts of israel. non-engorged questing adult ticks were collected from 13 localities. a total of 1196 ticks in 131 pools-83 pools of rhipicephalus turanicus and 48 of rhipicephalus sanguineus (with two to ...201120636417
why is southern african canine babesiosis so virulent? an evolutionary perspective.abstract: canine babesiosis is a common, highly virulent disease in southern africa with even pups and juveniles being severely affected. this contrasts with bovine babesiosis, for example, where host, parasite and vector co-evolved and young animals develop immunity after infection without showing clinical signs. babesia rossi, the main causative organism of canine babesiosis in sub-saharan africa, was first described from a side-striped jackal (canis adustus) in kenya. although data are meagre ...201121489239
babesiosis in dogs and cats-expanding parasitological and clinical spectra.canine babesiosis caused by different babesia species is a protozoal tick-borne disease with worldwide distribution and global significance. historically, babesia infection in dogs was identified based on the morphologic appearance of the parasite in the erythrocyte. all large forms of babesia were designated babesia canis, whereas all small forms of babesia were considered to be babesia gibsoni. however, the development of molecular methods has demonstrated that other babesia species such as ba ...201121571435
babesia canis rossi infection in a texas dog.a 5-month-old intact male boerboel dog, imported from south africa 1 week previously, was presented to a texas veterinarian for lethargy, anorexia, and labored breathing. the dog was febrile, anemic, leukopenic, thrombocytopenic, and slightly azotemic. results of the idexx snap-4dx enzyme immunoassay were negative for dirofilaria immitis antigen and antibodies against ehrlichia canis, borrelia burgdorferi, and anaplasma phagocytophilum. an edta blood sample analyzed at oklahoma state university ...201121790699
a survey of canine tick-borne diseases in india.abstract: background: there are few published reports on canine babesia, ehrlichia, anaplasma, hepatozoon and haemotropic mycoplasma infections in india and most describe clinical disease in individual dogs, diagnosed by morphological observation of the microorganisms in stained blood smears. this study investigated the occurrence and distribution of canine tick-borne disease (tbd) pathogens using a combination of conventional and molecular diagnostic techniques in four cities in india. results: ...201121771313
molecular detection of hepatozoon canis and babesia canis vogeli in domestic dogs from cuiabá, brazil.the objective of this study was to report for the first time infection by hepatozoon spp. and babesia spp. in 10 dogs from the city of cuiabá, state of mato grosso, central-western brazil. a pair of primers that amplifies a 574 bp fragment of the 18s rrna of hepatozoon spp., and a pair of primers that amplifies a 551 bp fragment of the gene 18s rrna for babesia spp. were used. six dogs were positive for babesia spp., and 9 were positive for hepatozoon spp. co‑infection of babesia spp. and hepato ...201121961759
epidemiological aspects on vector-borne infections in stray and pet dogs from romania and hungary with focus on babesia spp.canine arthropod-borne infections are of major interest in small animal practice and have been widely investigated in central and western europe. however, only limited epidemiological data are available from south-eastern european countries, although diseases including babesiosis or dirofilariosis are widely recognised as important canine infections in these countries. a steadily increasing number of dogs imported from south-eastern europe into germany require particular attention by small anima ...201121947342
molecular and serological detection of ehrlichia canis and babesia vogeli in dogs in colombia.ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by ehrlichia canis and babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, rhipicephalus sanguineus. the present work aimed to detect the presence of e. canis and babesia sp. in 91 dog blood samples in colombia, by molecular and serological techniques. we also performed sequence alignment to indicate the identity of the parasite species infecting these animals. the present wo ...201122130333
Canine babesiosis in Romania due to Babesia canis and Babesia vogeli: a molecular approach.Canine babesiosis is a tick-borne disease caused by the protozoa Babesia spp. that affects dogs worldwide. In Romania, canine babesiosis has become quite frequent in the last few years, with a wide variety of clinical signs, ranging from mild, nonspecific illness to peracute collapse, and even death. Traditionally, a Babesia infection in dogs is diagnosed based on the morphologic appearance of the intraerythrocytic piroplasms observed in peripheral blood smears. To date, no data on genetic chara ...201122006189
phylogenetic characterization of babesia canis vogeli in dogs in the state of goiás, brazil.the genus babesia comprises protozoa that cause diseases known as babesiosis. dogs are commonly affected by babesia canis or babesia gibsoni. babesia canis is divided into the subspecies babesia canis canis, babesia canis vogeli and babesia canis rossi. among these, babesia canis vogeli predominates in brazil. the objective of this study was to conduct a phylogenetic analysis on babesia isolates from dogs in goiânia, goiás. blood samples were obtained from 890 dogs presenting clinical signs sugg ...201122166380
detection of babesia vogeli in stray cats of metropolitan bangkok, thailand.the combination of a rapidly growing stray animal population and the lack of animal control in bangkok has resulted in a unique opportunity to evaluate the potential role of companion animals as sentinels and reservoirs of infectious diseases, including several of those caused by vector-borne parasites. the purpose of this study was to determine the prevalence and factors associated with the distribution of babesia species infections among stray cats in bangkok. blood samples were collected from ...201020638794
diagnosis of canine vector-borne diseases in young dogs: a longitudinal study.canine vector-borne diseases (cvbds) pose a diagnostic challenge, particularly when a dog is coinfected with more than one pathogen. the purpose of this study was to generate information about the diagnosis of cvbds in young dogs following their first exposure to flea, tick, sand fly, louse, and mosquito vectors. from march 2008 to may 2009, 10 purpose-bred young naive beagle dogs and a cohort of 48 mixed-breed dogs living in an area to which cvbd is endemic in southern italy were monitored usin ...201020660218
multiplex real-time qpcr for the detection of ehrlichia canis and babesia canis vogeli.ehrlichia canis and babesia canis vogeli are two tick-borne canine pathogens with a worldwide importance. both pathogens are transmitted by rhipicephalus sanguineus, the brown dog tick, which has an increasing global distribution. a multiplex quantitative real-time pcr (qpcr) assay for the simultaneous detection of the tick-borne pathogens e. canis and b. canis vogeli was developed using dual-labeled probes. the target genes were the 16s rrna of e. canis and the heat shock protein 70 (hsp70) of ...201020674177
molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan recife (north-eastern brazil).to identify dna of the main tick-borne pathogens in dogs from recife (brazil), polymerase chain reactions were carried out on blood samples of dogs treated at the veterinary hospital of the universidade federal rural de pernambuco from march 2007 to june 2008. the detection of dna was performed using specific primers. amplicons were analyzed through electrophoresis and sequencing. a phylogenetic tree was constructed using the upgma method, revealing that the sequences were closely related to tho ...201020680344
evolution of clinical, haematological and biochemical findings in young dogs naturally infected by vector-borne pathogens.longitudinal studies evaluating the evolution of clinical, haematological, biochemical findings in young dogs exposed for the first time to multiple vector-borne pathogens have not been reported. with the objective of assessing the evolution of clinical, haematological and biochemical findings, these parameters were serially monitored in naturally infected dogs throughout a 1-year follow-up period. young dogs, infected by vector-borne pathogens based on cytology or polymerase chain reaction, wer ...201021106311
a pcr study of piroplasms in 166 dogs and 111 horses in france (march 2006 to march 2008).positive pcr results for piroplasms were obtained on edta blood samples from 166 dogs and 111 horses between march 2006 and march 2008. the organisms were initially identified using common primers, followed by restriction enzyme profiles to determine the species and types of babesia/theileria. in 27 dogs and eight horses with positive results, the size of the specimen was insufficient to separate species/types. we identified babesia canis canis in 105 of the 166 dogs (63%) and theileria equi in ...201020221639
Refresh
firstprev
12
nextlast
Displaying items 1 - 100 of 136