Publications

molecular genetic analysis and evolution of begomoviruses and betasatellites causing yellow mosaic disease of bhendi.	in india, bhendi yellow vein mosaic disease (byvmd) is one of the most economically important diseases of bhendi/okra and is caused by a complex of monopartite begomovirus (bhendi yellow vein mosaic virus-byvmv) and betasatellite (bhendi yellow vein betasatellite-byvb). in this study, we have analyzed the role of possible evolutionary factors involved in the evolution of byvmv and byvb isolates. evidence of inter-species and inter-strain recombination events was detected among the viral isolates ...	2017	27891562
association of tomato leaf curl new delhi virus dna-b with bhendi yellow vein mosaic virus in okra showing yellow vein mosaic disease symptoms.	okra samples showing yellow vein mosaic, vein twisting and bushy appearance were collected from different locations of india during the surveys conducted between years 2005-2009. the dot blot and pcr detection revealed that 75.14% of the samples were associated with monopartite begomovirus and remaining samples with bipartite virus. whitefly transmission was established for three samples representing widely separated geographical locations which are negative to betasatellites and associated with ...	2015	26104329
development of a vigs vector based on the β-satellite dna associated with bhendi yellow vein mosaic virus.	bhendi yellow vein mosaic virus (bymv) is a monopartite begomovirus with an associated β-satellite. βc1 orf encoded by the β-satellite is the symptom determinant and a strong suppressor of post transcriptional gene silencing. to create a virus induced gene silencing vector based upon the β-satellite associated with byvmv the βc1 orf was replaced with multiple cloning sites. gfp transgene and plant endogenous genes su, pds, pcna and ago1 were cloned into β-satellite based vigs vector. gfp express ...	2015	25169741
genetic variability of cotton leaf curl betasatellite in northern india.	cotton is an important crop and its production is affected by various disease pathogens. monopartite begomovirus associated betasatellites cause cotton leaf curl disease (clcud) in northern india. in order to access the occurrence and genetic variability of cotton leaf curl betasatellites, an extensive field survey was conducted in states of rajasthan, punjab and haryana. we selected the betasatellite sequence for analysis as they are reported as important for disease severity and sequence varia ...	2014	25473373
the c2 protein of bhendi yellow vein mosaic virus plays an important role in symptom determination and virus replication.	bhendi yellow vein mosaic virus (byvmv) that causes bhendi yellow vein mosaic disease is a monopartite begomovirus with an associated betasatellite. previous studies have shown that c2 protein of byvmv acts as a suppressor of post transcriptional gene silencing, activates transcription, localizes to nucleus, and interacts with karyopherin α. to probe the role of c2 in symptom determination and virus replication, the infectious clones of byvmv containing two stop codons in the c2 orf were created ...	2014	24122068
begomovirus characterization, and development of phenotypic and dna-based diagnostics for screening of okra genotype resistance against bhendi yellow vein mosaic virus.	the leaf sample from okra plants showing the yellow vein mosaic disease symptoms was collected in karnataka state, india. the genome of the virus was amplified, cloned and sequenced. sequence analysis revealed that the viral genome (gu112065) is 2,741 bp in length and genome is similar to that of monopartite begomoviruses originating from the old world, with seven conserved orfs. further nucleotide (nts) sequence comparisons showed that the genome has the highest sequence identities of 96.1 % wi ...	2013	28324417
association of a recombinant cotton leaf curl bangalore virus with yellow vein and leaf curl disease of okra in india.	a begomovirus isolate (oy136a) collected from okra plants showing upward leaf curling, vein clearing, vein thickening and yellowing symptoms from bangalore rural district, karnataka, india was characterized. the sequence comparisons revealed that, this virus isolate share highest nucleotide identity with isolates of cotton leaf curl bangalore virus (clcubv) (ay705380) (92.8 %) and okra enation leaf curl virus (81.1-86.2 %). this is well supported by phylogentic analysis showing, close clustering ...	2013	24426275
molecular characterization of distinct bipartite begomovirus infecting bhendi (abelmoschus esculentus l.) in india.	yellow vein mosaic disease of okra is a whitefly transmitted begomovirus causing heavy economic loss in different parts of india. the okra isolate (oy131) of this virus from a bhendi plant [(abelmoschus esculentus l.) moench] showing yellow vein mosaic, vein twisting, reduced leaves, and a bushy appearance in the palem region, new delhi, india, was characterized in the present study. the complete dna-a and dna-b sequences have been determined and are comprised of 2,746 and 2,703 nucleotides, res ...	2012	22447131
mapping of functional region conferring nuclear localization and karyopherin α-binding activity of the c2 protein of bhendi yellow vein mosaic virus.	bhendi yellow vein mosaic disease is caused by a complex consisting of a monopartite begomovirus associated with a β-satellite. the c2 protein of bhendi yellow vein mosaic virus (byvmv) is a suppressor of post-transcriptional gene silencing and also functions as a transcriptional activator. to explore the molecular mechanisms of its nuclear trafficking and self-interaction, fusion proteins of fluorescent proteins with wild-type or mutated constructs of byvmv c2 were expressed in tobacco protopla ...	2012	22357749
differential roles of c4 and betac1 in mediating suppression of post-transcriptional gene silencing: evidence for transactivation by the c2 of bhendi yellow vein mosaic virus, a monopartite begomovirus.	bhendi yellow vein mosaic disease (byvmd) is caused by the association of a dna beta satellite with a begomovirus component. the begomovirus component has two promoters, one in the virion sense (v-sense) and the other in the complementary sense (c-sense) in the intergenic region (ir). to study the promoter activities of v-sense and c-sense promoters, mgfp gene fusion was made downstream to the promoters. transient and stable expressions in n. benthamiana leaves showed significant gfp expression ...	2007	16949698
protein-protein interactions and nuclear trafficking of coat protein and betac1 protein associated with bhendi yellow vein mosaic disease.	bhendi yellow vein mosaic disease (byvmd) is caused by a complex consisting of a monopartite begomovirus byvmv and a satellite dna beta component. byvmv represents a new member of the emerging group of monopartite begomoviruses requiring a satellite component for symptom induction. here we report the results of the transient expression of green fluorescent protein (gfp) fused with the betac1 and coat protein (cp) coding regions, in the epidermal cells of nicotiana benthamiana. gfpcp was found to ...	2006	16934356
bhendi yellow vein mosaic disease in india is caused by association of a dna beta satellite with a begomovirus.	yellow vein mosaic disease is the major limitation in the production of bhendi or okra (abelmoschus esculentus), an important vegetable crop of india. this disease is caused by a complex consisting of the monopartite begomovirus bhendi yellow vein mosaic virus (byvmv, family: geminiviridae) and a small satellite dna beta component. byvmv can systemically infect bhendi upon agroinoculation but produces only mild leaf curling in this host. dna beta induces typical symptoms of bhendi yellow vein mo ...	2003	12573576