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aspergillus awamori-fermented mung bean seed coats enhance the antioxidant and immune responses of weaned pigs.the potential benefits of aspergillus-fermented mung bean seed coats (fmsc) for weaned pigs remain unexplored. both in vitro and in vivo experiments were employed to evaluate the potential of fmsc supplement on the growth, antioxidant and immune responses of weaned pigs. the total polyphenols and dpph scavenging capability of ethanol extract of fmsc exhibited a greater (p < 0.01) increase than those of pre-fermentation. with the addition of the polyphenol of fmsc extract, an increase in phagocyt ...201728063238
bioactive profile, free-radical scavenging potential, dna damage protection activity, and mycochemicals in aspergillus awamori (mtcc 548) extracts: a novel report on filamentous fungi.biomass of aspergillus awamori was investigated for mycochemicals, total phenolic compounds (tpc), condensed tannin content (ctc), free-radical scavenging potential (frsp), and dna damage protection activity. frsp was determined using dpph, abts, frap (ferric reducing antioxidant power), metal chelating activity, and cupric reducing antioxidant capacity) assays. water (aq), aqueous ethanol 50% (aqe), and methanol were used as extraction phase at 44.5 °c for 23.8 min. aqe shows the presence of ma ...201728660460
the role of soybean extracts and isoflavones in hormone-dependent breast cancer: aromatase activity and biological effects.estrogen receptor-positive (er(+)) breast cancer is the most common cause of cancer death in women worldwide. nowadays, the relationship between soya diet and breast cancer is controversial due to the unknown role of its isoflavones, genistein (g) and daidzein (d). in this work, we investigated not only the anti-tumor properties of a soybean extract (nbse) but also whether the biotransformation of extract (bse) by the fungus aspergillus awamori increased its effectiveness. the bse showed a stron ...201728644496
biochemical characterization of the xylan hydrolysis profile of the extracellular endo-xylanase from geobacillus thermodenitrificans t12.endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. hemicellulose degradation by geobacillus spp. is facilitated by the hemicellulose utilization (hus) locus that is present in most strains belonging to this genus. as part of the hus locus, the xyna gene encoding an extracellular endo-xylanase is one of the few secreted enzymes and considered to be the key enzyme to initiate hemicellulose degradation. several geobacillus endo-xylanases have been charact ...201728521816
enhanced biodiesel production through phyco-myco co-cultivation of chlorella minutissima and aspergillus awamori: an integrated approach.algae-fungus co-culture was investigated as an alternative biodiesel feedstock. an oleaginous filamentous fungus aspergillus awamori was co-cultured with chlorella minutissima mcc 27 and chlorella minutissima utex 2219, respectively in n11 medium furnished with different carbon and nitrogen sources. the biomass and lipid production potential of the two c. minutissima-a. awamori co-cultures was compared against the monocultures. a substantial enhancement in biomass and lipid accumulation was obse ...201728475992
production, immobilization and thermodynamic studies of free and immobilized aspergillus awamori amylase.enzyme cost, stability and its thermodynamic characteristics are the main criteria for industrial use. in this study, aspergillus awamori amylase was constitutively produced using various agro-industrial wastes. olive oil cake gave the highest activity (230u/g). the amylase was partially purified to 2.81-fold purification. immobilization was achieved using different carriers by covalent binding. the novel carrier ca(+2) alginate (alg) starch (st)/polyethyleneimine (pei)/glutaraldehyde (ga), show ...201728438682
determination and optimization of vitamin b complex in xylanase enzyme treated polished rice by response surface methodology.the present study provides information about the concentrations of vitamin b (thiamine, riboflavin, pyridoxine and niacin) in polished brown rice treated with xylanase. xylanase enzyme was produced from aspergillus awamori mtcc 9166. brown rice was treated with 60-100% enzyme (40 ml of buffer -undiluted) for 30 to 150 min (with variation of 30 min) at 30 degrees c to 50 degrees c (with variation of 5 degrees c) to attain a saturated moisture level of 35.5 g100(-1)g .the enzyme acted upon selecti ...201627498499
studies on organic and in-organic biostimulants in bioremediation of diesel-contaminated arable soil.in this study, use of inorganic fertilizer (n.p.k) was compared with organic manure (compost) in the bioremediation of diesel-polluted agricultural soil over a two-month period. renewal by enhanced natural attenuation was used as control. the results revealed that total petroleum hydrocarbon removal from polluted soil was 71.40 ± 5.60% and 93.31 ± 3.60% for n.p.k and compost amended options, respectively. the control (natural attenuation) had 57.90 ± 3.98% of total petroleum hydrocarbon removed. ...201627494315
transformation of litchi pericarp-derived condensed tannin with aspergillus awamori.condensed tannin is a ubiquitous polyphenol in plants that possesses substantial antioxidant capacity. in this study, we have investigated the polyphenol extraction recovery and 2,2-diphenyl-1-picrylhydrazyl (dpph) scavenging activity of the extracted polyphenol after litchi pericarp is treated with aspergillus awamori, aspergillus sojae or aspergillus oryzae. we have further explored the activity of a. awamori in the formation of condensed tannin. the treatment of a. awamori appeared to produce ...201627420043
biohydrogen production in the suspended and attached microbial growth systems from waste pastry hydrolysate.waste pastry was hydrolyzed by glucoamylase and protease which were obtained from solid state fermentation of aspergillus awamori and aspergillus oryzae to produce waste pastry hydrolysate. then, the effects of hydraulic retention times (hrts) (4-12h) on hydrogen production rate (hpr) in the suspended microbial growth system (continuous stirred tank reactor, cstr) and attached microbial growth system (continuous mixed immobilized sludge reactor, cmisr) from waste pastry hydrolysate were investig ...201627416509
species distribution and in vitro azole susceptibility of aspergillus section nigri isolates from clinical and environmental settings.aspergillus section nigri includes species of interest for animal and human health, although studies on species distribution are limited to human cases. data on the antifungal susceptibilities and the molecular mechanism of triazole resistance in strains belonging to this section are scant. forty-two black aspergillus strains from human patients (16 isolates), animals (14 isolates), and the environment (12 isolates) were molecularly characterized and their in vitro triazole susceptibilities inve ...201627413191
valorization of bakery waste for biocolorant and enzyme production by monascus purpureus.a concept of utilizing bakery waste as a nutrient source for the fermentative production of bio-colorant by monasucus purpureus has been developed. the proposed ideas provide an innovative approach to establish a system or method to reduce the bakery waste problem encountered by hong kong or other countries. bakery waste collected from bakery store was used in submerged and solid-state fermentation of monascus purpureus to produce bio-colorant, which could potentially be applied in food and text ...201627208753
continuous biohydrogen production from waste bread by anaerobic sludge.in this study, continuous biohydrogen production from waste bread by anaerobic sludge was performed. the waste bread was first hydrolyzed by the crude enzymes which were generated by aspergillus awamori and aspergillus oryzae via solid-state fermentation. it was observed that 49.78g/l glucose and 284.12mg/l free amino nitrogen could be produced with waste bread mass ratio of 15% (w/v). the waste bread hydrolysate was then used for biohydrogen production by anaerobic sludge in a continuous stirre ...201627065225
aspergillus tubingensis and aspergillus niger as the dominant black aspergillus, use of simple pcr-rflp for preliminary differentiation.this work aimed to identify the species distribution of common clinical and environmental isolates of black aspergilli based on simple restriction fragment length polymorphism (rflp) analysis of the β-tubulin gene. a total of 149 clinical and environmental strains of black aspergilli were collected and subjected to preliminary morphological examination. total genomic dnas were extracted, and pcr was performed to amplify part of the β-tubulin gene. at first, 52 randomly selected samples were spec ...201626852194
prospecting for the incidence of genes involved in ochratoxin and fumonisin biosynthesis in brazilian strains of aspergillus niger and aspergillus welwitschiae.aspergillus niger "aggregate" is an informal taxonomic rank that represents a group of species from the section nigri. among a. niger "aggregate" species aspergillus niger sensu stricto and its cryptic species aspergillus welwitschiae (=aspergillus awamori sensu perrone) are proven as ochratoxin a and fumonisin b2 producing species. a. niger has been frequently found in tropical and subtropical foods. a. welwitschiae is a new species, which was recently dismembered from the a. niger taxon. these ...201626803270
optimization of d-lactic acid production using unutilized biomass as substrates by multiple parallel fermentation.this study investigated the optimization of d-lactic acid production from unutilized biomass, specifically banana peel and corncob by multiple parallel fermentation (mpf) with leuconostoc mesenteroides and aspergillus awamori. the factors involved in mpf that were assessed in this study comprised banana peel and corncob, kh2po4, tween 80, mgso4·7h2o, nacl, yeast extract, and diammonium hydrogen citrate to identify the optimal concentration for d-lactic acid production. optimization of these comp ...201628330258
immobilization of halophilic aspergillus awamori em66 exochitinase on grafted k-carrageenan-alginate beads.a novel extreme halophilic exochitinase enzyme was produced by honey isolate aspergillus awamori em66. the enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (ca) with 93 % immobilization yield. the immobilization process significantly improved the enzyme specific activity 2.6-fold compared to the free form. the significant factors influencing the immobilization process such as enzyme protein concentration and loading time were studied. distinguishable characteristics of o ...201628330097
antidiabetic potential of a peptide isolated from an endophytic aspergillus awamori.to exploit the potential of endophytic fungi for pharmaceutically important antidiabetic alpha glycosidase inhibitors.201626544796
biodegradation kinetics of thin-stillage treatment by aspergillus awamori and characterization of recovered chitosan.an attempt has been made to provide solution for distillery wastewater using fungal pretreatment followed by an anaerobic process to achieve higher organic matter removal, which is a challenge at present with currently adopted technologies. submerged growth kinetics of distillery wastewater supernatant by aspergillus awamori was also evaluated. the proposed kinetic models using a logistic equation for fungal growth and the leudeking-piret equation for product formation were validated experimenta ...201626512009
[influence of uv-radiation on physical-chemical and functional properties of free immobilized glucoamylase from aspergillus awamori].the effect of uv light (240-390 nm) at the doses of 151-1510 j/m2 on the catalytic activity of free and im- mobilized glucoamylase from aspergillus awamori was investigated. it was established that the loss of catalytic activity of the enzyme was associated with photochemical transformations of trp-120, which is part of the active site of glucoamylase. it was shown that the use of collagen as a carrier for immobilization of glucoamylase reduces the constant photoinactivation of the enzyme. it wa ...201625507622
[influence of uv-radiation on physical-chemical and functional properties of free immobilized glucoamylase from aspergillus awamori].the effect of uv light (240-390 nm) at the doses of 151-1510 j/m2 on the catalytic activity of free and im- mobilized glucoamylase from aspergillus awamori was investigated. it was established that the loss of catalytic activity of the enzyme was associated with photochemical transformations of trp-120, which is part of the active site of glucoamylase. it was shown that the use of collagen as a carrier for immobilization of glucoamylase reduces the constant photoinactivation of the enzyme. it wa ...201625427372
effects of an aglycone-rich biotransformed soybean extract in human endothelial cells.estrogen replacement therapy is not thought to be a safe treatment for prevention of cardiovascular disease in menopausal women; isoflavones are a possible alternative. estrogen produces beneficial effects on the cardiovascular system by enhancing production of nitric oxide, a vasoprotective and antiatherosclerotic agent. estrogen-like compounds such as isoflavones are also suggested for increasing nitric oxide production. isoflavones are present mainly in soy foods as glucosides, but soy isofla ...201525530207
enhancing organic matter removal, biopolymer recovery and electricity generation from distillery wastewater by combining fungal fermentation and microbial fuel cell.for enhancing organic matter removal from cereal-based distillery stillage two-stage treatment consisting of fermentation by aspergillus awamori followed by microbial fuel cell (mfc) is proposed. considerable reduction in total and soluble chemical oxygen demand (cod) up to 70% and 40%, respectively, along with 98% reduction of suspended solids (ss) has been achieved during fungal pretreatment. the process generated chitosan, a useful fermentation byproduct from fungal mycelia, as 0.6-0.7g/l of ...201525460978
multivariate optimization and supplementation strategies for the simultaneous production of amylases, cellulases, xylanases, and proteases by aspergillus awamori under solid-state fermentation conditions.the production of extracts containing a pool of enzymes for extensive biomass deconstruction can lead to significant advantages in biorefinery applications. in this work, a strain of aspergillus awamori ioc-3914 was used for the simultaneous production of five groups of hydrolases by solid-state fermentation of babassu cake. sequential experimental design strategies and multivariate optimization using the desirability function were first used to study temperature, moisture content, and granulome ...201525413792
xylan degradation improved by a combination of monolithic columns bearing immobilized recombinant β-xylosidase from aspergillus awamori x-100 and grindamyl h121 β-xylanase.synergistic action of exo- and endohydrolazes is preferred for effective destruction of biopolymers. the main purpose of the present work was to develop an efficient tool for degradation of xylan. macroporous lab-made monolithic columns and commercial cim-epoxy disk were used to immobilize the recombinant β-xylosidase from aspergillus awamori and grindamyl β-xylanase. the efficiency of xylan degradation using the low-loaded β-xylosidase column appeared to be four times higher than for the in-sol ...201525367775
valorisation of mixed bakery waste in non-sterilized fermentation for l-lactic acid production by an evolved thermoanaerobacterium sp. strain.in this study, an advanced biorefinery technology that uses mixed bakery waste has been developed to produce l-lactic acid using an adaptively evolved thermoanaerobacterium aotearoense la1002-g40 in a non-sterilized system. under these conditions, mixed bakery waste was directly hydrolysed by aspergillus awamori and aspergillus oryzae, resulting in a nutrient-rich hydrolysate containing 83.6g/l glucose, 9.5 g/l fructose and 612 mg/l free amino nitrogen. t. aotearoense la1002-g40 was evaluated an ...201526363501
the method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolysis of p-nitrophenyl glycosides.in the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters kcat, km and product ki for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. its applicability was demonstrated for three retaining exo-glycoside hydrolases, β-xylosidase from aspergillus awamori, β-galactosidase from penicillium sp. and α-galactosidase from thermotoga maritima (tmgala). during the analysis of ...201526005928
batch dark fermentation from enzymatic hydrolyzed food waste for hydrogen production.a combination bioprocess of solid-state fermentation (ssf) and dark fermentative hydrogen production from food waste was developed. aspergillus awamori and aspergillus oryzae were utilized in ssf from food waste to generate glucoamylase and protease which were used to hydrolyze the food waste suspension to get the nutrients-rich (glucose and free amino nitrogen (fan)) hydrolysate. both glucose and fan increased with increasing of food waste mass ratio from 4% to 10% (w/v) and the highest glucose ...201525978853
[changes in growth patterns and intracellular calcium concentrations in aspergillus awamori treated with amphotericin b].growth patterns and intracellular ca2+ concentrations in the mutant strain aspergillus awamori 66a, containing a recombinant aequorin gene were studied in the presence of a permeabilizing fungicidal agent amphotericin b. the cell response, i.e., changes in the growth and development of the fungus (initiation of spore germination, mycelial growth, and intensity ofsporulation) was dose-dependent. low concentrations of amphotericin b (2.5 microm) stimulated spore germination: the number of germinat ...201525844454
effect of feeding aspergillus awamori and canola seed on the growth performance and muscle fatty acid profile in broiler chicken.the aim of this study was to examine effects of dietary supplementation with aspergillus awamori and feeding canola seed on the growth and fatty acid profile in broilers. twenty-eight chicks (15 days old) were assigned to the following groups: (1) control, fed a basal diet; (2) awamori, fed the basal diet supplemented with 0.05% a. awamori; (3) canola, fed a diet containing 5% canola seed; and (4) canola + awamori, fed the canola diet supplemented with a. awamori (seven birds/group). body weight ...201525773115
untreated chlorella homosphaera biomass allows for high rates of cell wall glucan enzymatic hydrolysis when using exoglucanase-free cellulases.chlorophyte microalgae have a cell wall containing a large quantity of cellulose iα with a triclinic unit cell hydrogen-bonding pattern that is more susceptible to hydrolysis than that of the cellulose iβ polymorphic form that is predominant in higher plants. this study addressed the enzymatic hydrolysis of untreated chlorella homosphaera biomass using selected enzyme preparations, aiming to identify the relevant activity profile for the microalgae cellulose hydrolysis. enzymes from acremonium c ...201525763103
a 3-vinyl cephem derivative, a useful intermediate in the synthesis of cepham antibiotics, from aspergillus awamori associated with banana fruit.aspergillus awamori was isolated from a diseased banana fruit, musa acuminata cv. ambul. the fungus was fermented in potato dextrose broth and on potato dextrose agar media and the fungal media were extracted with etoac. chromatographic separation of the etoac extracts furnished 4-methoxybenzyl 7-phenylacetamido-3-vinyl-3-cephem-4-carboxylate (1), along with three naphtho-γ-pyrones, flavasperone (2), foncesinone a (3) and aurasperone a (4), and three alkaloids, aspernigrin a (5), pestalamide c ( ...201526669099
[introduction of additional thiol groups into glucoamylase in aspergillus awamori and their effect on the thermal stability and catalytic activity of the enzyme].five mutant forms of glucoamylase (ga) from the filamentous fungus aspergillus awamori with artificial disulfide bonds (4d-g137a\a14c, 6d-a14c\y419c\g137a, 10d-v13c\g396c, 11d-v13c\g396c\a14c\y419c\g137a, and 20d-g137a\a246c\a14c) were constructed using computer simulation and experimentally tested for thermostability. the introduction of two additional disulfide bonds between its first and thirteenth alpha-helices and that of the loop located close to a catalytic residue--e400--made it possible ...201425272730
formulation of fermentation media from flour-rich waste streams for microbial lipid production by lipomyces starkeyi.flour-rich waste (frw) and by-product streams generated by bakery, confectionery and wheat milling plants could be employed as the sole raw materials for generic fermentation media production, suitable for microbial oil synthesis. wheat milling by-products were used in solid state fermentations (ssf) of aspergillus awamori for the production of crude enzymes, mainly glucoamylase and protease. enzyme-rich ssf solids were subsequently employed for hydrolysis of frw streams into nutrient-rich ferme ...201425150217
mixed food waste as renewable feedstock in succinic acid fermentation.mixed food waste, which was directly collected from restaurants without pretreatments, was used as a valuable feedstock in succinic acid (sa) fermentation in the present study. commercial enzymes and crude enzymes produced from aspergillus awamori and aspergillus oryzae were separately used in hydrolysis of food waste, and their resultant hydrolysates were evaluated. for hydrolysis using the fungal mixture comprising a. awamori and a. oryzae, a nutrient-complete food waste hydrolysate was genera ...201425149459
structural analysis of cerebrosides from aspergillus fungi: the existence of galactosylceramide in a. oryzae.glucosylceramide and galactosylceramide were detected in three aspergillus species: aspergillus oryzae, aspergillus sojae and aspergillus. awamori, using borate-coated tlc. the cerebrosides from a. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determined the composition of sugar, fatty acid and sphingoid base by gc/ms, maldi-tof/ms and (1)h-nmr. we identified them as β-glucosylceramide and β-galactosylceramide. the ceramide moiety ...201425129050
comparison of species composition and fumonisin production in aspergillus section nigri populations in maize kernels from usa and italy.fumonisin contamination of maize is considered a serious problem in most maize-growing regions of the world, due to the widespread occurrence of these mycotoxins and their association with toxicosis in livestock and humans. fumonisins are produced primarily by species of fusarium that are common in maize grain, but also by some species of aspergillus sect. nigri, which can also occur on maize kernels as opportunistic pathogens. understanding the origin of fumonisin contamination in maize is a ke ...201425087207
biotransformed soybean extract (bse) inhibits melanoma cell growth and viability in vitro: involvement of nuclear factor-kappa b signaling.melanoma is recognized as one of the most aggressive cancers with a relatively high propensity for metastasis. the prognosis of melanoma remains poor in spite of treatment advances, emphasizing the importance of additional preventive measures. isoflavonoids have become not only potential chemopreventive, but also important therapeutic natural agents. we evaluated the antiproliferative and proapoptotic properties of biotransformed soybean extract (bse) in a375 melanoma cells. previous analyses de ...201425072850
upgrading the antioxidant potential of cereals by their fungal fermentation under solid-state cultivation conditions.solid-state fermentation (ssf) at 30°c for 72 h with four generally recognized as safe (gras) filamentous fungi (aspergillus oryzae ncim 1212, aspergillus awamori mtcc no. 548, rhizopus oligosporus ncim 1215 and rhizopus oryzae rck2012) showed high efficiency for the improvement of water-soluble total phenolic content (tpc) and antioxidant properties including abts(●+) [2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid)] and dpph(●) (2,2'-diphenyl-1-picrylhydrazyl) scavenging capacities of ...201424964826
application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification.polygalacturonase and α-amylase play vital role in fruit juice industry. in the present study, polygalacturonase was produced by aspergillus awamori nakazawa mtcc 6652 utilizing apple pomace and mosambi orange (citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from a. oryzae (ifo-30103) using wheat bran by solid state fermentation (ssf) process. these carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charc ...201424948919
integrative effects of feeding aspergillus awamori and fructooligosaccharide on growth performance and digestibility in broilers: promotion muscle protein metabolism.this study was conducted to show the effect of aspergillus awamori (aa), fructooligosaccharide (fos), and combined aspergillus awamori and fructooligosaccharide (aa + fos) on growth, digestibility, blood parameters, and expression of some growth-related genes. a total of 60 broiler chicks at the age of 15 d were divided into a control group (n = 15) and 3 treatment groups. the control group was fed a basal diet, and the treatment groups were fed basal diets supplemented with 0.05% aa, 0.05% fos, ...201424895630
antifungal activity and identification of lactobacilli, isolated from traditional dairy product "katak".filamentous moulds are the main spoilage microorganisms, responsible for significant economic losses and several healthy risks in human food chain. the lactic acid bacteria (lab), especially lactobacilli could be a natural antagonist of these dangerous organisms. in bulgaria, a very limited data exists on the antifungal activity of lab microbiota of fermented dairy products. in the present study, four active strains were isolated from traditional fermented curd/yogurt-like product "katak", produ ...201424887637
operating conditions for the continuous bioremediation of free cyanide contaminated wastewater using aspergillus awamori.generation of cyanide-containing wastewater is a growing problem worldwide as numerous cyanide complexes are highly unstable and degrade to form free cyanide (f-cn), the most toxic form of cyanide. agro-waste materials, such as sweet orange (citrus sinensis) waste from the citrus industry, are rich in readily metabolisable carbohydrates that can supplement microbial activity and thus support biodegradation of toxic compounds in wastewater. this study reports on optimal operating conditions for t ...201424622547
fungal hydrolysis in submerged fermentation for food waste treatment and fermentation feedstock preparation.potential of fungal hydrolysis in submerged fermentation by aspergillus awamori and aspergillus oryzae as a food waste treatment process and for preparation of fermentation feedstock has been investigated. by fungal hydrolysis, 80-90% of the initial amount of waste was reduced and degraded within 36-48 h into glucose, free amino nitrogen (fan) and phosphate. experiments revealed that 80-90% of starch can be converted into glucose and highest concentration of fan obtained, when solid mashes of a. ...201424583214
production of quercetin, kaempferol and their glycosidic derivatives from the aqueous-organic extracted residue of litchi pericarp with aspergillus awamori.our previous work exhibited aspergillus awamori fermentation of the litchi pericarp increased significantly antioxidant activity and dna protection effect. in this present study, the litchi pericarp and its aqueous-organic extracted residues were fermented by a. awamori in order to elucidate the enhanced beneficial effects. the study identified that rutin which present in litchi pericarp could be deglycosylated to form quercetin and quercetin-3-glucoside after the fermentation. application the s ...201424128471
fermentative polyhydroxybutyrate production from a novel feedstock derived from bakery waste.in this study, halomonas boliviensis was cultivated on bakery waste hydrolysate and seawater in batch and fed-batch cultures for polyhydroxybutyrate (phb) production. results demonstrated that bakery waste hydrolysate and seawater could be efficiently utilized by halomonas boliviensis while phb contents between 10 and 30% (w/w) were obtained. furthermore, three methods for bakery waste hydrolysis were investigated for feedstock preparation. these include: (1) use of crude enzyme extracts from as ...201425136626
modern taxonomy of biotechnologically important aspergillus and penicillium species.taxonomy is a dynamic discipline and name changes of fungi with biotechnological, industrial, or medical importance are often difficult to understand for researchers in the applied field. species belonging to the genera aspergillus and penicillium are commonly used or isolated, and inadequate taxonomy or uncertain nomenclature of these genera can therefore lead to tremendous confusion. misidentification of strains used in biotechnology can be traced back to (1) recent changes in nomenclature, (2 ...201424377856
taxonomic re-evaluation of black koji molds.black koji molds including its albino mutant, the white koji mold, have been widely used for making the distilled spirit shochu in northeast asia because they produce citric acid which prevents undesirable contamination from bacteria. since inui reported aspergillus luchuensis from black koji in okinawa in 1901, many fungal names associated with black koji molds were reported. however, some species are similar and differentiation between species is difficult. fungal taxonomists tried to arrange ...201424281756
comparative study of biochemical properties of glucoamylases from the filamentous fungi penicillium and aspergillus.here we report the first isolation to homogeneous forms of two glucoamylases from the fungus penicillium verruculosum and their study in comparison with known glucoamylases from aspergillus awamori and aspergillus niger. genes that encode glucoamylases from p. verruculosum were cloned and expressed in the fungus penicillium canescens, and the recombinant glucoamylases were obtained with subsequent study of their molecular weights, isoelectric points, optimal temperature and ph values, and stabil ...201324237153
efficacy of root-associated fungi and pgpr on the growth of pisum sativum (cv. arkil) and reproduction of the root-knot nematode meloidogyne incognita.the effects of root-associated fungi (aspergillus awamori and glomus mosseae) and plant growth promoting rhizobacteria (pgpr) (pseudomonas putida, pseudomonas alcaligenes and paenibacillus polymyxa) were studied alone and in combination in glasshouse experiments on the growth of pea, enzyme activity (peroxidase and catalase) and reproduction of root-knot nematode meloidogyne incognita. application of a. awamori, g. mosseae and pgpr caused a significant increase in pea growth and enzyme activitie ...201322915081
efficient production of lignocellulolytic enzymes xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by the mutant strain aspergillus awamori 2b.361 u2/1.the production of xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by aspergillus awamori 2b.361 u2/1, a hyper producer of glucoamylase and pectinase, was evaluated using selected conditions regarding nitrogen nutrition. submerged cultivations were carried out at 30 °c and 200 rpm in growth media containing 30 g wheat bran/l as main carbon source and either yeast extract, ammonium sulfate, sodium nitrate or urea, as nitrogen sources; in all cases it was used a fixed molar carbon t ...201324294256
characterization of antifungal activity of the gh-46 subclass iii chitosanase from bacillus circulans mh-k1.we characterized the antifungal activity of the bacillus circulans subclass iii mh-k1 chitosanase (mh-k1 chitosanase), which is one of the most intensively studied glycoside hydrolases (ghs) that belong to gh family 46. mh-k1 chitosanase inhibited the growth of zygomycetes fungi, rhizopus and mucor, even at 10 pmol (0.3 μg)/ml culture probably via its fungistatic effect. the amino acid substitution e37q abolished the antifungal activity of mh-k1 chitosanase, but retained binding to chitotriose. ...201323892828
aspergillus awamori feeding modifies lipid metabolism in rats.in the present study, an experiment was conducted to show that a. awamori modifies lipid metabolism in mammals. a total number of 24 rats at 6 weeks of age were divided into 2 groups (10% and 30% fat dietary groups), and each group was further divided into control and experimental groups (6 rats per group). rats in the experimental groups were given diets containing 0.05% a. awamori. the diets were administered for 3 weeks to evaluate the effects of a. awamori on growth, plasma lipid profile, an ...201323841078
phenol biodegradation by fungal cells immobilized in sol-gel hybrids.the capability of cells of the fungus aspergillus awamori, either free or immobilized in hybrid sol-gel material cells, for phenol biodegradation was demonstrated. phenol was present in the reaction mixture as the sole carbon and energy source, and its decomposition was followed in repeated batch degradation experiments. atomic force microscopy provided information on the development of self-organizing structures in the materials synthesized by the sol-gel method. phenol biodegradation was media ...201323659173
food waste as nutrient source in heterotrophic microalgae cultivation.glucose, free amino nitrogen (fan), and phosphate were recovered from food waste by fungal hydrolysis using aspergillus awamori and aspergillus oryzae. using 100g food waste (dry weight), 31.9 g glucose, 0.28 g fan, and 0.38 g phosphate were recovered after 24h of hydrolysis. the pure hydrolysate has then been used as culture medium and nutrient source for the two heterotrophic microalgae schizochytrium mangrovei and chlorella pyrenoidosa, s. mangrovei and c. pyrenoidosa grew well on the complex ...201323587816
distribution and mycotoxigenic potential of aspergillus section nigri species in naturally contaminated almonds.in a previous study, inedible almond pick-out samples were assayed for aflatoxin and aflatoxigenic aspergillus species. these samples contained high populations of black-spored aspergillus section nigri species. to investigate whether these species may contribute to the total potential mycotoxin content of almonds, aspergillus section nigri strains were isolated from these samples and assayed for ochratoxin a (ota) and fumonisin b2 (fb2). the majority of isolates (117 strains, 68%) were identifi ...201323575138
improved growth of lactobacillus bulgaricus and streptococcus thermophilus as well as increased antioxidant activity by biotransforming litchi pericarp polysaccharide with aspergillus awamori.this study was conducted to increase the bioactivity of litchi pericarp polysaccharides (lpps) biotransformed by aspergillus awamori. compared to the non-a. awamori-fermented lpp, the growth effects of a. awamori-fermented lpp on lactobacillus bulgaricus and streptococcus thermophilus were four and two times higher after 3 days of fermentation, respectively. increased 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and dna protection activity of litchi pericarp polysaccharides were als ...201323484117
burdock fermented by aspergillus awamori elevates cecal bifidobacterium, and reduces fecal deoxycholic acid and adipose tissue weight in rats fed a high-fat diet.this study investigated the effects of dietary supplementation with burdock powder and aspergillus awamori-fermented burdock powder at 5% on the intestinal luminal environment and body fat in rats fed a high-fat (hf) diet. food intake and growth were unaffected by dietary manipulation. consumption of the burdock and fermented burdock diets significantly elevated fecal iga and mucins (indices of intestinal immune and barrier functions) and reduced fecal lithocholic acid (a risk factor for colon c ...201323291748
enhancing the antitumor cell proliferation and cu(2+)-chelating effects of black soybeans through fermentation with aspergillus awamori.in the present study, black soybeans were fermented with aspergillus awamori at 30°c for 3 days. the effect of fermentation on the antiproliferative effect against human colon cancer cells, caco-2 and ht-29 as well as cu(2+)-chelating effect of black soybeans was investigated. it was found that the water, 80% methanol or 80% ethanol extract of fermented black soybeans showed a significantly higher (p < 0.05) antiproliferative and cu(2+)-chelating effect than did the respective extract of non-fer ...201323177213
functional assembly and characterization of a modular xylanosome for hemicellulose hydrolysis in yeast.five trimeric xylanosomes were successfully assembled on the cell surface of saccharomyces cerevisiae. three dockerin-tagged fungal enzymes, an endoxylanase (xynac) from thermomyces lanuginosus, a β-xylosidase (xlndt) from aspergillus niger and an acetylxylan esterase (awaxef) from aspergillus awamori, were displayed for the synergistic saccharification of birchwood xylan. the surface-expression scaffoldins were modular constructs with or without carbohydrate binding modules from thermotoga mari ...201322806709
bioaugmented composting of jatropha de-oiled cake and vegetable waste under aerobic and partial anaerobic conditions.this study was conducted to assess the effect of microbial inoculation in jatropha cake composting with different vegetable waste. the microbial inoculums composed of fungal strains (aspergillus awamori, aspergillus nidulans, trichoderma viride, phanerochaete chrysosporium) and bacterial inoculums (pseudomonas striata as phosphorus solublizer and azotobacter chroococcum as nitrogen fixer) were added to the compost mixture after the thermophilic phase was over for bioaugmenting of jatropha cake u ...201322736484
intensification of fructosyltransferases and fructo-oligosaccharides production in solid state fermentation by aspergillus awamori ghrts.the present work was aimed to investigate the impact of the solid substrates mixture on fructosyltransferases (ftase) and fructo-oligosaccharides (fos) production. an augmented simplex lattice design was used to optimize a three component mixture for ftase production. among selected substrates corn cobs has highest impact on ftase production followed by wheat bran and rice bran. all two substrates and three substrate combinations showed the highest enzyme production than their individual levels. ...201324426134
effect of phosphate-solubilizing fungi aspergillus awamori s29 on mungbean (vigna radiata cv. rmg 492) growth.a phosphate solubilizing fungus, aspergillus awamori s29 was isolated from rhizoshpere of mungbean. the phosphate solubilizing activity of a. awamori s29 in liquid was 1,110 mg/l for tricalcium phosphate (tcp). the organism was able to solubilize various inorganic forms of phosphate at a wide range of temperatures. among various insoluble phosphate sources tested, di-calcium phosphate was solubilized the most, followed by tcp. a. awamori s29 had significant effect (p < 0.05) on mungbean growth, ...201222661080
codon-optimized glucoamylase sgai of aspergillus awamori improves starch utilization in an industrial yeast.the development of a yeast that converts raw starch to ethanol in one step (called consolidated bioprocessing) could yield large cost reductions in the bioethanol industry. the aim of this study was to develop an efficient amylolytic saccharomyces cerevisiae strain suitable for industrial bioethanol production. a native and codon-optimized variant of the aspergillus awamori glucoamylase gene were expressed in the s. cerevisiae y294 laboratory strain. codon optimization resulted to be effective a ...201222450569
the modification of the muscle fatty acid profile by dietary supplementation with aspergillus awamori in broiler chickens.the present study was conducted to show that dietary supplementation with a fungus, aspergillus awamori, modifies muscle fatty acid profiles in broiler chickens. a total of thirty chicks, selected from a group of 100 chicks aged 15 d, were divided into a control group and two treatment groups (ten birds per treatment). the control group was fed a basal diet, and the treatment groups were fed basal diets supplemented with a. awamori at levels of 0·05 and 0·2 %. from the start of the study at 15 d ...201222289635
casein phosphopeptides drastically increase the secretion of extracellular proteins in aspergillus awamori. proteomics studies reveal changes in the secretory pathway.the secretion of heterologous animal proteins in filamentous fungi is usually limited by bottlenecks in the vesicle-mediated secretory pathway.201222234238
enhanced dpph radical scavenging activity and dna protection effect of litchi pericarp extract by aspergillus awamori bioconversion. 201223016522
feeding aspergillus awamori reduces skeletal muscle protein breakdown and stimulates growth in broilers.this study was conducted to show that dietary supplementation of a fungus, aspergillus awamori called koji in japan, reduces skeletal muscle protein breakdown and stimulates growth in broiler chickens. a total of 30 chicks at 15 days of age was divided into control and two treatment groups (10 birds per treatment). control group was fed basal diet and treatment groups were fed the basal diets supplemented with a. awamori at levels of 0.05% and 0.2%. the birds were raised for 12 days from 15 to 2 ...201222862929
xylanase production by aspergillus awamori under solid state fermentation conditions on tomato pomace.in this work, tomato pomace, a waste abundantly available in the mediterranean and other temperate climates agro-food industries, has been used as raw material for the production of some hydrolytic enzymes, including xylanase, exo-polygalacturonase (exo-pg), cellulase (cmcase) and α-amylase. the principal step of the process is the solid state fermentation (ssf) of this residue by aspergillus awamori. in several laboratory experiments, maximum xylanase and exo-pg activities were measured during ...201124031793
garcina cambogia leaf and seawater for tannase production by marine aspergillus awamori btmfw032 under slurry state fermentation.garcinia gummi-gutta (syn. g. cambogia, g. quaesita), known to have medicinal properties, was evaluated as a substrate and inducer for tannase production by a marine aspergillus awamori btmfw032, under slurry state fermentation using czapekdox-minimal medium and sea water as the cultivation medium. among the various natural tannin substrates evaluated, garcinia leaf supported maximal tannase production. the cultivation conditions and components of the cultivation medium were optimized employing ...201122312743
influence of different substrates on the production of a mutant thermostable glucoamylase in submerged fermentation.three mutations, ser54→pro, thr314→ala, and his415→tyr, were identified in aspergillus awamori glucoamylase gene expressed by saccharomyces cerevisiae. the mutant glucoamylase (ga) was substantially more thermostable than a wild-type ga at 70 °c, with a 3.0 kj mol(-1) increase in the free energy of thermo-inactivation. the effect of starch from different botanical sources on the production of this ga was measured in liquid fermentation using commercial soluble starch, cassava, potato, and corn a ...201120414741
propyl gallate synthesis using acidophilic tannase and simultaneous production of tannase and gallic acid by marine aspergillus awamori btmfw032.marine aspergillus awamori btmfw032, recently reported by us, produce acidophilic tannase as extracellular enzyme. here, we report the application of this enzyme for synthesis of propyl gallate by direct transesterification of tannic acid and in tea cream solubilisation besides the simultaneous production of gallic acid along with tannase under submerged fermentation by this fungus. this acidophilic tannase enabled synthesis of propyl gallate by direct transesterification of tannic acid using pr ...201121279470
fermentation with aspergillus awamori enhanced contents of amino nitrogen and total phenolics as well as the low-density lipoprotein oxidation inhibitory activity of black soybeans.a solid fermentation was performed on black soybeans with aspergillus awamori. the effects of fermentation on the contents of total phenolics and amino nitrogen and on the inhibitory effect on low-density lipoprotein (ldl) oxidation of black soybeans were examined. results revealed that fermentation significantly enhanced the ldl oxidation inhibitory activity and total phenolics and amino nitrogen contents of black soybeans. the increased content of amino nitrogen was closely related to the enha ...201121370926
raw starch fermentation to ethanol by an industrial distiller's yeast strain of saccharomyces cerevisiae expressing glucoamylase and α-amylase genes.industrial strains of a polyploid, distiller's saccharomyces cerevisiae that produces glucoamylase and α-amylase was used for the direct fermentation of raw starch to ethanol. strains contained either aspergillus awamori glucoamylase gene (ga1), debaryomyces occidentalis glucoamylase gene (gam1) or d. occidentalis α-amylase gene (amy), singly or in combination, integrated into their chromosomes. the strain expressing both ga1 and amy generated 10.3% (v/v) ethanol (80.9 g l(-1)) from 20% (w/v) ra ...201121479627
lipase from marine aspergillus awamori btmfw032: production, partial purification and application in oil effluent treatment.marine fungus btmfw032, isolated from seawater and identified as aspergillus awamori, was observed to produce an extracellular lipase, which could reduce 92% fat and oil content in the effluent laden with oil. in this study, medium for lipase production under submerged fermentation was optimized statistically employing response surface method toward maximal enzyme production. medium with soyabean meal-0.77% (w/v); (nh(4))(2)so(4)-0.1m; kh(2)po(4)-0.05m; rice bran oil-2% (v/v); cacl(2)-0.05m; peg ...201121549226
synthesis of sugar fatty acid esters using a ß-xylosidase from aspergillus awamori for transxylosylation.aspergillus awamori k4 ß-xylosidase has broad acceptor specificity. it has been used to synthesize a sugar fatty acid ester via its transxylosylation activity. one xylosyl residue was initially transferred to hexamethylene glycol as a linker with a yield of 0.36 g/g xylobiose. linoleic acid was subsequently linked to one terminal hydroxyl side of the transfer product hydroxyhexyl xyloside through an esterification reaction catalyzed by a lipase. the synthesis of hexyl linoleoyl xyloside was conf ...201121805184
[creation of a heterologous gene expression system on the basis of aspergillus awamori recombinant strain].a heterologous gene expression system was created in a domestic aspergillus awamori co-6804 strain, which is a producer of the glucoamylase gene. vector pga was prepared using promoter and terminator areas of the glucoamylase gene, and a. niger phytase, trichoderma reesei endoglucanase, and penicillium canescens xylanase genes were then cloned into pga vector. separation of enzyme samples using fplc showed the amount of the recombinant proteins to be within the 0.6-14% range of total protein.201121790031
Species assignment and antifungal susceptibilities of black aspergilli recovered from otomycosis cases in Iran.Black aspergilli are among the main causative agents of otomycosis worldwide. In this study, the species assignment of black aspergilli isolated from otomycosis cases in Iran was carried out using sequence analysis of part of the calmodulin gene. The results indicate that Aspergillus niger is not the only black Aspergillus species involved in otomycosis cases in Iran: Aspergillus awamori and Aspergillus tubingensis are also able to cause ear infections. Antifungal susceptibility tests were carri ...201121895787
Development of an efficient gene-targeting system in Aspergillus luchuensis by deletion of the non-homologous end joining system.The industrial fungus Aspergillus luchuensis is used to produce a distilled spirit in Okinawa Island, Japan. Recently, the genome sequence of A. luchuensis RIB2604 (Aspergillus awamori NBRC 4314) was revealed and many functional genes are now expected to be analyzed. Gene targeting is necessary for analyzing the function of a gene; however, gene targeting frequencies in A. luchuensis are very low. To develop a highly efficient gene-targeting system for A. luchuensis, we disrupted A. luchuensis l ...201121903470
Aspergillus niger contains the cryptic phylogenetic species A. awamori.Aspergillus section Nigri is an important group of species for food and medical mycology, and biotechnology. The Aspergillus niger 'aggregate' represents its most complicated taxonomic subgroup containing eight morphologically indistinguishable taxa: A. niger, Aspergillus tubingensis, Aspergillus acidus, Aspergillus brasiliensis, Aspergillus costaricaensis, Aspergillus lacticoffeatus, Aspergillus piperis, and Aspergillus vadensis. Aspergillus awamori, first described by Nakazawa, has been compar ...201122036292
multiresponse optimization of inoculum conditions for the production of amylases and proteases by aspergillus awamori in solid-state fermentation of babassu cake.this work aimed at investigating the simultaneous production of amylases and proteases by solid-state fermentation (ssf) of babassu cake using aspergillus awamori ioc-3914. by means of experimental design techniques and the desirability function, optimum inoculum conditions (c/n ratio of propagation medium, inoculum age, and concentration of inoculum added to ssf medium) for the production of both groups of enzymes were found to be 25.8, 28.4 h, and 9.1 mg g(-1), respectively. significant influe ...201121915371
study of conformational changes in glucoamylase of aspergillus awamori nakazawa in presence of denaturants through cd-spectroscopy.in present study, changes in the pattern or motifs of secondary structures of glucoamylase from aspergillus awamori nakazawa in the presence of denaturants such as urea, guanidine-hcl (gdn-hcl), and at different ph, were studied through cd-spectroscopy. it was observed that in native state glucoamylase entirely comprised of beta-sheets. cd-spectra of glucoamylase in the presence of 6 m urea lost its spectral regular characteristic; and ellipticity became zero between 207 and 218 nm. with 0.5 m u ...201020452763
purification and characterization studies of a thermostable β-xylanase from aspergillus awamori.this study presents data on the production, purification, and properties of a thermostable β-xylanase produced by an aspergillus awamori 2b.361 u2/1 submerged culture using wheat bran as carbon source. fractionation of the culture filtrate by membrane ultrafiltration followed by sephacryl s-200 and q-sepharose chromatography allowed for the isolation of a homogeneous xylanase (pxii-1), which was 32.87 kda according to ms analysis. the enzyme-specific activity towards soluble oat spelt xylan, whi ...201020549295
recombinant human lactoferrin: a valuable protein for pharmaceutical products and functional foods.lactoferrin, the main iron-binding protein of milk, has biological activities that are essential for the newborn and are beneficial for adults. given this beneficial effect, there is broad interest in exogenous sources of lactoferrin in human nutrition. consequently, several transgenic approaches to produce lactoferrin have been achieved. however, the activity of heterologous lactoferrin cannot be assumed to identically mimic that of the homologous protein. human lactoferrin obtained from yeast, ...201020624450
sugarcane bagasse enzymatic hydrolysis: rheological data as criteria for impeller selection.the aim of this work was to select an efficient impeller to be used in a stirred reactor for the enzymatic hydrolysis of sugar cane bagasse. all experiments utilized 100 g (dry weight)/l of steam-pretreated bagasse, which is utilized in brazil for cattle feed. the process was studied with respect to the rheological behavior of the biomass hydrolysate and the enzymatic conversion of the bagasse polysaccharides. these parameters were applied to model the power required for an impeller to operate a ...201020844924
acidophilic tannase from marine aspergillus awamori btmfw032.aspergillus awamori btmfw032, isolated from sea water, produced tannase as extracellular enzyme under submerged culture conditions. enzyme with a specific activity of 2761.89 iu/mg protein, a final yield of 0.51 %, and a purification fold of 6.32 was obtained after purification to homogeneity by ultrafiltration and gel filtration. sds-page analyses under non- reducing and reducing conditions yielded a single band of 230 kda and 37.8 kda, respectively, indicating presence of six identical monomer ...201021030825
economic analysis of the production of amylases and other hydrolases by aspergillus awamori in solid-state fermentation of babassu cake.amylases are one of the most important industrial enzymes produced worldwide, with their major application being in ethanol manufacturing. this work investigated the production of amylases by solid-state fermentation of babassu cake, using the filamentous fungus aspergillus awamori ioc-3914. lab-scale experiments were carried out to generate input data for simulations of an industrial plant for amylase production. additionally to the target enzymes, other hydrolases (cellulases, xylanases, and p ...201021048867
[increase in glucoamylase productivity of aspergillus awamori strain by combination of radiating mutagenesis and plasmid transformation methods].increase in the level of amylolytic genes activator protein encoded by amyr gene was shown to result in enhancement of glucoamylase productivity of a. awamori strain by 30%. however, the same effect equal to 30% increase can be achieved by introduction of extra copies of gla gene encoding glucoamylase. these two effects were not additive, which gave the possibility to suggest an additional limitation in the egulation mechanism of glucoamylase gene expression in aspergillus family strains while i ...201021261079
[antibacterial activity of secondary metabolites from aspergillus awamori f12 isolated from rhizospheric soil of rhizophora stylosa griff].to identify aspergillus awamori strain f12 isolated from rhizospheric soil of rhizophora stylosa griff and to characterize antibacterial compounds from the ethyl acetate extracts of its fermentation broth.201021141475
characterization of a chimeric enzyme comprising feruloyl esterase and family 42 carbohydrate-binding module.we engineered a chimeric enzyme (awfaea-cbm42) comprising of type-a feruloyl esterase from aspergillus awamori (awfaea) and family 42 carbohydrate-binding module (akcbm42) from glycoside hydrolase family 54 alpha-l-arabinofuranosidase of aspergillus kawachii. the chimeric enzyme was successfully produced in pichia pastoris and accumulated in the culture broth. the purified chimeric enzyme had an apparent relative molecular mass (m(r)) of 53,000. the chimeric enzyme binds to arabinoxylan; this in ...201019756576
effect of additives on the activity of tannase from aspergillus awamori mtcc9299.tannase from aspergillus awamori mtcc 9299 was purified using ammonium sulfate precipitation followed by ion-exchange chromatography. a purification fold of 19.5 with 13.5% yield was obtained. temperature of 30 degrees c and ph of 5.5 were found optimum for tannase activity. the effects of metals and organic solvents on the activity of tannase were also studied. metal ions mg(+2), mn(+2), ca(+2), na(+), and k(+) stimulated the tannase activity, while cu(+2), fe(+3), and co(+2) acted as inhibitor ...201019844665
new oxidized sterols from aspergillus awamori and the endo-boat conformation adopted by the cyclohexene oxide system.two new oxidized sterols 1 and 2 were obtained from the active fraction of a mangrove fungus aspergillus awamori isolated from the soils around the mangrove plant acrostichum speciosum. their structures were elucidated using spectroscopic methods as 22e-7alpha-methoxy-5alpha,6alpha-epoxyergosta-8(14),22-dien-3beta-ol (1) and 22e-3beta-hydroxy-5alpha,6alpha,8alpha,14alpha-diepoxyergosta-22-en-7-one (2). the nmr data and complete assignments for both dmso-d(6) and cdcl(3) were given. their cytotox ...201019877128
endo- and exo-inulinases: enzyme-substrate interaction and rational immobilization.three-dimensional models of exoinulinase from bacillus stearothermophilus and endoinulinase from aspergillus niger were built up by means of homology modeling. the crystal structure of exoinulinase from aspergillus awamori was used as a template, which is the sole structure of inulinase resolved so far. docking and molecular dynamics simulations were performed to investigate the differences between the two inulinases in terms of substrate selectivity. the analysis of the structural differences b ...201019941325
expression of feruloyl esterase from aspergillus awamori in escherichia coli: characterization and crystal studies of the recombinant enzyme.this paper describes cloning, expression, characterization and crystallization of feruloyl esterase from aspergillus awamori. the gene coding for feruloyl esterase from a. awamori was amplified, cloned, and expressed in escherichia coli. the recombinant enzyme was purified, characterized and used for crystallization. its x-ray crystallographic data were collected and explained using molecular replacement theory. also, the tertiary structure of the recombinant enzyme-ferulic acid complex was also ...201020138908
[the effect of point amino acid substitutions in an internal alpha-helix on thermostability of aspergillus awamori x100 glucoamylase].conformational flexibility of alpha-helices in glucoamylase of the fungus aspergillus awamori was studied by molecular dynamics methods. several amino acid substitutions (g127a, p128a, i136l, g137a, and g139a) optimizing intrinsic interactions in one of the alpha-helices (d) within the hydrophobic core of this protein were constructed and studied. it was found that these point mutations had different effects on the glucoamylase thermal inactivation constant. unlike amino acid substitution p128a ...201020391767
fungal isolates from natural pectic substrates for polygalacturonase and multienzyme production.pectin rich wastes and waste dump yard soils were screened and eighty pectinolytic fungal isolates were obtained by enrichment culturing and ruthenium red plate assay. eight isolates with higher zones of pectin hydrolysis were selected and tested for polygalacturonase production. one isolate identified as aspergillus awamori mtcc 9166 with highest polygalacturonase activity was tested for utilization of raw pectins for enzyme production. polygalacturonase production was high in raw pectin source ...201023100850
purification and characterization of a low molecular weight endo-xylanase from mushroom termitomyces clypeatus.a low molecular weight endo-xylanase (ec 3.2.1.8) was purified from an edible mushroom termitomyces clypeatus grown in submerged medium with oat spelt xylan. xylanase was purified to apparent homogeneity by ammonium sulfate fractionation and gel filtration chromatography. its molecular weight was determined by gel filtration chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 12 kda. the enzyme was found to be most active at 50 degrees c and ph 5.0, being most stab ...201019728166
construction of a direct starch-fermenting industrial strain of saccharomyces cerevisiae producing glucoamylase, alpha-amylase and debranching enzyme.to develop a strain of saccharomyces cerevisiae that produces ethanol directly from starch, two integrative vectors were constructed to allow the simultaneous multiple integration of the aspergillus awamori glucoamylase gene (ga1) and the debaryomyces occidentalis alpha-amylase gene (amy) and glucoamylase with debranching activity gene (gam1) into the chromosomes of an industrial strain of s. cerevisiae. the ga1 and amy genes were constitutively expressed under the adc1 promoter in s. cerevisiae ...201020131079
effect of heat treatment on total phenolic and anthocyanin contents as well as antioxidant activity of the extract from aspergillus awamori-fermented black soybeans, a healthy food ingredient.aspergillus awamori-fermented black soybeans, a healthy food ingredient, were subjected to heating at 40-100 degrees c for 30 min. after heat treatment, the methanol extract of the fermented black soybeans was examined for antioxidant activity including the 2,2-diphenyl-1-picryl-hydrozyl radical-scavenging effect and the fe(2+)-chelating ability. it was found that antioxidant activity exerted by the methanol extract was stable with heating at a temperature up to 80 degrees c for 30 min. the extr ...200919817642
enhanced in vitro and in vivo antioxidant activity and mobilization of free phenolic compounds of soybean flour fermented with different beta-glucosidase-producing fungi.to evaluate the soybean polyphenol glucosides bioconversion to aglycone forms by different beta-glucosidases-producing filamentous fungi to enhance their antioxidant activity.200919200313
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