Publications

transcriptomic and metabolomic profiles of chinese citrus fly, bactrocera minax (diptera: tephritidae), along with pupal development provide insight into diapause program.	the chinese citrus fly, bactrocera minax (enderlein), is a devastating citrus pest in asia. this univoltine insect enters obligatory pupal diapause in each generation, while little is known about the course and the molecular mechanisms of diapause. in this study, the course of diapause was determined by measuring the respiratory rate throughout the pupal stage. in addition, the variation of transcriptomic and metabolomic profiles of pupae at five developmental stages (pre-, early-, middle-, late ...	2017	28704500
rna interference of a trehalose-6-phosphate synthase gene reveals its roles during larval-pupal metamorphosis in bactrocera minax (diptera: tephritidae).	trehalose is the major blood sugar in insects, which plays a crucial role as an instant source of energy and the starting substrate for chitin biosynthesis. in insects, trehalose is synthesized by catalysis of an important enzyme, trehalose-6-phosphate synthase (tps). in the present study, a trehalose-6-phosphate synthase gene from bactrocera minax (bmtps) was cloned and characterized. bmtps contained an open reading frame of 2445 nucleotides encoding a protein of 814 amino acids with a predicte ...	2017	27405007
de novo transcriptome analysis of chinese citrus fly, bactrocera minax (diptera: tephritidae), by high-throughput illumina sequencing.	the chinese citrus fly, bactrocera minax (enderlein), is one of the most devastating pests of citrus in the temperate areas of asia. so far, studies involving molecular biology and physiology of b. minax are still scarce, partly because of the lack of genomic information and inability to rear this insect in laboratory. in this study, de novo assembly of a transcriptome was performed using illumina sequencing technology. a total of 20,928,907 clean reads were obtained and assembled into 33,324 un ...	2016	27331903
small-scale spatio-temporal distribution of bactrocera minax (enderlein) (diptera: tephritidae) using probability kriging.	understanding spatio-temporal distribution of pest in orchards can provide important information that could be used to design monitoring schemes and establish better means for pest control. in this study, the spatial and temporal distribution of bactrocera minax (enderlein) (diptera: tephritidae) was assessed, and activity trends were evaluated by using probability kriging. adults of b. minax were captured in two successive occurrences in a small-scale citrus orchard by using food bait traps, wh ...	2016	26935208
pupal diapause termination in bactrocera minax: an insight on 20-hydroxyecdysone induced phenotypic and genotypic expressions.	the chinese citrus fruit fly, bactrocera minax, is an economically important pest of citrus. it exhibits pupal diapause from november to may to combat harsh environmental conditions. such a long pupal diapause is a barrier for laboratory rearing and development of control strategies against this pest. in the present study, 20-hydroxyecdysone (20e) was used to break pupal diapause of b. minax by topical application. after diapause termination by 20e treated, the pupal ontogenetic processes were o ...	2016	27273028
the effects of rna interference targeting bactrocera dorsalis ds-bdrpl19 on the gene expression of rpl19 in non-target insects.	double-stranded rna (dsrna) designed to target pest genes emerges as a promising strategy for improving pest control. therefore, it is necessary to assess the effects of dsrna on non-target insects, such as native enemies and beneficial insects, to determine the environmental safety of such treatments. in this paper, we investigated the effects of dsrna targeting rpl19 from bactrocera dorsalis on non-target insects in citrus ecological systems by feeding the dsrna to bactrocera minax, apis melli ...	2015	25567188
effect of six insecticides on three populations of bactrocera (tetradacus) minax (diptera: tephritidae).	the chinese citrus fruit fly, bactrocera minax is one of the most economically important and aggressive pests threatening the chinese citrus industry. in order to provide some recommendations for the improvement of integrated pest management of this species, the authors evaluated the toxicity of 6 insecticides on the second stage larvae, fourth stage larvae, and adult flies from multiple geographical b. minax populations. in addition, the influences of each pesticide on pupation and emergence we ...	2015	25564253
effects of juvenile hormone analogue and ecdysteroid on adult eclosion of the fruit fly bactrocera minax (diptera: tephritidae).	the univoltine bactrocera minax (enderlein) is a devastating pest of citrus in nepal, india, bhutan, and china. to better understand the overwintering pupal diapause, we investigated the juvenile hormone iii (jh iii) and ecdysteroid 20-hydroxyecdysone (20e) titers across the developmental stages, and the effects of juvenile hormone analog (jha) and 20e treatments on adult emergence patterns. the results showed that both jh iii and 20e levels fluctuated from the late larval stage to the late pupa ...	2014	25195444
the complete nucleotide sequence of the mitochondrial genome of bactrocera minax (diptera: tephritidae).	the complete 16,043 bp mitochondrial genome (mitogenome) of bactrocera minax (diptera: tephritidae) has been sequenced. the genome encodes 37 genes usually found in insect mitogenomes. the mitogenome information for b. minax was compared to the homologous sequences of bactrocera oleae, bactrocera tryoni, bactrocera philippinensis, bactrocera carambolae, bactrocera papayae, bactrocera dorsalis, bactrocera correcta, bactrocera cucurbitae and ceratitis capitata. the analysis indicated the structure ...	2014	24964138
transcriptome characterization analysis of bactrocera minax and new insights into its pupal diapause development with gene expression analysis.	bactrocera minax is a major citrus pest distributed in china, bhutan and india. the long pupal diapause duration of this fly is a major bottleneck for artificial rearing and underlying mechanisms remain unknown. genetic information on b. minax transcriptome and gene expression profiles are needed to understand its pupal diapause. high-throughput rna-seq technology was used to characterize the b. minax transcriptome and to identify differentially expressed genes during pupal diapause development. ...	2014	25285037
three heat shock protein genes from bactrocera (tetradacus) minax enderlein: gene cloning, characterization, and association with diapause.	bactrocera (tetradacus) minax enderlein is a major pest to wild and cultivated species of citrus. bactrocera minax produces one generation per year with a long pupal diapause period of over 6 months, which hinders efforts to obtain vast numbers of insects under standard room conditions. determining the mechanisms of diapause is significantly important for obtaining large quantities of these insects. to characterize the heat shock protein (hsp) genes of b. minax and to unravel their potential con ...	2014	27193815
bacterial communities in the gut and reproductive organs of bactrocera minax (diptera: tephritidae) based on 454 pyrosequencing.	the citrus fruit fly bactrocera minax is associated with diverse bacterial communities. we used a 454 pyrosequencing technology to study in depth the microbial communities associated with gut and reproductive organs of bactrocera minax. our dataset consisted of 100,749 reads with an average length of 400 bp. the saturated rarefaction curves and species richness indices indicate that the sampling was comprehensive. we found highly diverse bacterial communities, with individual sample containing a ...	2014	25215866
field evaluation of attractive lures for the fruit fly bactrocera minax (diptera: tephritidae) and their potential use in spot sprays in hubei province (china).	the chinese citrus fruit fly, bactrocera minax (enderlein) is a univoltine tephritidae pest that infests citrus species. field trials were conducted in 2010 to determine the potential use of a lure based on enzymatical-hydrolyzed beer yeast as liquid bait (hereafter named h-protein bait) for b. minax in the hubei province, china. in a citrus orchard, we compared the attractiveness among aqueous solutions of h-protein bait, gf-120 fruit fly bait, sugar-vinegar-wine mixture, torula yeast, and jufe ...	2012	22928307
attractiveness of various combinations of colors and shapes to females and males of bactrocera minax (diptera: tephritidae) in a commercial mandarin grove in bhutan.	bactrocera minax (enderlein) (diptera: tephritidae) is a major pest of citrus fruit in the region from nepal through to southwestern china. in tests on wild adult populations of b. minax in a mandarin, citrus reticulata blanco, orchard in western bhutan, both males and females were more attracted to 50-mm-diameter spheres than to 50-mm discs of the same color. furthermore, they were more attracted to spheres colored with orange or green-yellow mixtures than to similar spheres colored red, yellow ...	2006	17066795
seasonal phenology of bactrocera minax (diptera: tephritidae) in western bhutan.	the chinese citrus fruit fly, bactrocera (tetradacus) minax (enderlein), is one of the major citrus pests in bhutan and can cause >50% mandarin (citrus reticulata blanco) fruit drop. as part of the development of a management strategy for the fly in mandarin orchards, population monitoring and experimental manipulations were carried out to determine: (i) adult emergence period; (ii) adult phenology patterns; (iii) period of crop susceptibility; and (iv) period from fruit drop to pupation. in wes ...	2006	17092364