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effects of biochar on phytotoxicity and translocation of polybrominated diphenyl ethers in ni/fe bimetallic nanoparticle-treated soil.in this study, soil culture experiments were conducted to explore the effects of biochar-supported ni/fe nanoparticles on the accumulation and translocation of polybrominated diphenyl ethers (pbdes) in soil-plant system and its phytotoxicity to brassica chinensis. compared with those in bde209 contaminated soils (s 1) and ni/fe nanoparticle-treated soil (s 3), the plant biomass, root, and shoot lengths in biochar-supported ni/fe nanoparticle-treated soil (s 4) were increased by 23 mg, 1.35 cm, a ...201829128943
dynamics of multiple elements in fast decomposing vegetable residues.litter decomposition regulates the cycling of nutrients and toxicants but is poorly studied in farmlands. to understand the unavoidable in-situ decomposition process, we quantified the dynamics of c, h, n, as, ca, cd, cr, cu, fe, hg, k, mg, mn, na, ni, pb, and zn during a 180-d decomposition study in leafy lettuce (lactuca sativa var. longifoliaf) and rape (brassica chinensis) residues in a wastewater-irrigated farmland in northwestern china. different from most studied natural ecosystems, the m ...201829100695
comparing the influence of selenite (se4+) and selenate (se6+) on the inhibition of the mercury (hg) phytotoxicity to pak choi.selenite (se (iv)) and selenate (se (iv)) have recently been demonstrated to be equally effective in inhibiting mercury (hg) phytotoxicity to plants. this assertion is still unclear. in this study, we aimed to explore the potential effects of se species (se4+ and se6+) on the inhibition of the mercury (hg) bioavailability to pak choi in dry land. pot experiments with exposure to different dosages of mercuric chloride (hgcl2) and selenite (na2seo3) or selenate (na2seo4) were treated. to compare t ...201828968942
comparison of dgt with traditional extraction methods for assessing arsenic bioavailability to brassica chinensis in different soils.several predictive models and methods have been used for heavy metals bioavailability, but there is no universally accepted approach in evaluating the bioavailability of arsenic (as) in soil. the technique of diffusive gradients in thin-films (dgt) is a promising tool, but there is a considerable debate with respect to its suitability. the dgt method was compared with other traditional chemical extractions techniques (soil solution, nahco3, nh4cl, hcl, and total as method) for estimating as bioa ...201829032263
effects of split applications of nitrogen fertilizers on the cd level and nutritional quality of chinese cabbage.cadmium (cd) contamination in soil is an increasingly serious problem. management of plant nutrients has been proposed as a potentially promising strategy for minimizing cd accumulation in crops grown in contaminated soil. this study investigated the effects of split applications of nitrogen (n) fertilizers on the cd concentration in chinese cabbage (brassica chinensis l.) plants grown in cd-contaminated soil. compared with single applications, split applications of ammonium or urea resulted in ...201728990380
over-expression of mir158 causes pollen abortion in brassica campestris ssp. chinensis.we identified and cloned the two precursors of mir158 and its target gene in brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. further study revealed that over-expression of mir158 caused reduced pollen varbility, which was caused by the degradation of pollen contents from the binucleate microspore stage. these results first suggest the role of mir158 in pollen development of brassica campestris ssp. chinensis. micrornas (mirnas) play crucial role ...201727909970
comparison of dgt with traditional methods for assessing cadmium bioavailability to brassica chinensis in different soils.there is no universally accepted method for evaluating cadmium (cd) bioavailability in soil. the diffusive gradient in thin films (dgt) technique is a promising tool, but there is considerable debate about its suitability. the ability of this technique to estimate cd bioavailability in soils was compared with the abilities of other traditional chemical extraction techniques (soil solution, ethylene diamine tetraacetic acid (edta), acetic acid (hac), calcium chloride (cacl2), and pseudo-total cd ...201729079727
development of est-ssr markers in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) based on de novo transcriptomic assemblies.flowering chinese cabbage is one of the most important vegetable crops in southern china. genetic improvement of various agronomic traits in this crop is underway to meet high market demand in the region, but the progress is hampered by limited number of molecular markers available in this crop. this study aimed to develop est-ssr markers from transcriptome sequences generated by next-generation sequencing. rna-seq of eight cabbage samples identified 48,975 unigenes. of these unigenes, 23,267 we ...201728902884
bioaccessibility and human exposure assessment of cadmium and arsenic in pakchoi genotypes grown in co-contaminated soils.in many countries cadmium (cd) and arsenic (as) commonly coexist in soils contaminated by mining activities, and can easily enter the human body via consumption of leafy vegetables, like the popularly consumed pakchoi (brassica chinensis l.), causing major health concerns. in the present study, bioaccessibility and human exposure of cd and as were assessed in twenty genotypes of pakchoi cultured at two different levels of co-contamination to identify low health risk genotypes. the bioaccessibili ...201728850097
effect of calcium bentonite on zn and cu mobility and their accumulation in vegetable growth in soil amended with compost during consecutive planting.in this study, greenhouse pot experiment was carried out to investigate the cu and zn mobility and their accumulations in pakchoi (brassica chinensis l.) and chinese cabbage (brassica rapachinensis l.) growth in ca-bentonite-pig manure co-compost (cbc)-soil system during three consecutive planting. five composts (0, 2.5, 5, 7.5, and 10% ca-bentonite-pig manure co-composts) amended soil with 5% (w/w) application rate and compared to control without any additive. the results showed that the additi ...201728523619
elevation of secondary metabolites synthesis in brassica campestris ssp. chinensis l. via exogenous inoculation of piriformospora indica with appropriate fertilizer.this work evaluated the impact of exogenous soil inoculation of beneficial fungal strain piriformospora indica on phytochemical changes and the related genes expression of chinese cabbage (brassica campestris ssp. chinensis l.) by greenhouse pot experiments. high performance liquid chromatography (hplc) affirmed that among the different combinations of fungal and organic fertilizer treatments, the phenolic acids and flavonoids were considerably enriched in organic fertilizer and fungi (op) follo ...201728493970
transcriptomic profiling of two pak choi varieties with contrasting anthocyanin contents provides an insight into structural and regulatory genes in anthocyanin biosynthetic pathway.the accumulation of anthocyanin in horticultural crops not only improves their stress tolerances but also their nutritional values. many key regulatory and structural genes in anthocyanin biosynthesis have been identified in model plants, but limited information is available for non-model plant species featured with colored leaves. in this study, two pak choi varieties with green or purple leaves were selected to analyze the anthocyanin biosynthesis through rna-seq.201728399809
plant growth, antibiotic uptake, and prevalence of antibiotic resistance in an endophytic system of pakchoi under antibiotic exposure.antibiotic contamination in agroecosystems may cause serious problems, such as the proliferation of various antibiotic resistant bacteria and the spreading of antibiotic resistance genes (args) in the environment or even to human beings. however, it is unclear whether environmental antibiotics, antibiotic resistant bacteria, and args can directly enter into, or occur in, the endophytic systems of plants exposed to pollutants. in this study, a hydroponic experiment exposing pakchoi (brassica chin ...201729099753
elevated co2 levels enhance the uptake and metabolism of organic nitrogen.the effects of elevated co2 (eco2 ) on the relative uptake of inorganic and organic nitrogen (n) are unclear. the uptake of different n sources by pak choi (brassica chinensis l.) seedlings supplied with a mixture of nitrate, glycine and ammonium was studied using 15 n-labelling under ambient co2 (aco2 ) (350 ppm) or eco2 (650 ppm) conditions. 15 n-labelled short-term uptake and 15 n-gas chromatography mass spectrometry (gc-ms) were applied to measure the effects of eco2 on glycine uptake and me ...201729080266
hexavalent chromium stress enhances the uptake of nitrate but reduces the uptake of ammonium and glycine in pak choi (brassica chinensis l.).chromium (cr) pollution affects plant growth and biochemical processes, so, the relative uptake of glycine, nitrate, and ammonium by pak choi (brassica chinensis) seedlings in treatments with 0mgl-1 and 10mgl-1 cr (vi) were detected by substrate-specific 15n-labelling in a sterile environment. the short-term uptake of 15n-labelled sources and 15n-enriched amino acids were detected by gas chromatography mass spectrometry to explore the mechanism by which cr stress affects glycine uptake and metab ...201728189780
[results of salad machine experiment within the mars-500 project].the salad machine experiment was aimed to fulfill performance testing of a prototype of space conveyor-type cylindrical greenhouse phytocycle-sl, to study growth and development of plants, and to evaluate microbial contamination of equipment in the closed manned environment. crops of leaf cabbage brassica chinensis l., cultivar vesnianka were raised in the time interval between mars-500 days 417 and 515. the greenhouse proved it serviceability demanding 17 min/(man x d) in the normal mode. most ...201723405423
sulfur protects pakchoi (brassica chinensis l.) seedlings against cadmium stress by regulating ascorbate-glutathione metabolism.cadmium (cd) pollution in food chains pose a potential health risk for humans. sulfur (s) is a significant macronutrient that plays a significant role in the regulation of plant responses to diverse biotic and abiotic stresses. however, no information is currently available about the impact of s application on ascorbate-glutathione metabolism (asa-gsh cycle) of pakchoi plants under cd stress. the two previously identified genotypes, namely, aikangqing (a cd-tolerant cultivar) and qibaoqing (a cd ...201728933771
the influence of particle size and feedstock of biochar on the accumulation of cd, zn, pb, and as by brassica chinensis l.biochar produced from rice straw (rc) and maize stalk (mc) was amended to the heavy metal-contaminated soil to investigate the effects of different biochar feedstock and particle size (fine, moderate, coarse) on the accumulation of cd, zn, pb, and as in brassica chinensis l. (chinese cabbage). the concentrations of cd, zn, and pb in shoot were decreased by up to 57, 75, and 63%, respectively, after biochar addition (4%). only mc decreased as concentration in b. chinensis l. shoots by up to 61%. ...201728801768
isolation and characterization of a phosphorus-solubilizing bacterium from rhizosphere soils and its colonization of chinese cabbage (brassica campestris ssp. chinensis).phosphate-solubilizing bacteria (psb) can promote the dissolution of insoluble phosphorus (p) in soil, enhancing the availability of soluble p. thus, their application can reduce the consumption of fertilizer and aid in sustainable agricultural development. from the rhizosphere of chinese cabbage plants grown in yangling, we isolated a strain of psb (yl6) with a strong ability to dissolve p and showed that this strain promoted the growth of these plants under field conditions. however, systemati ...201728798725
abscisic acid treatment alleviates cadmium toxicity in purple flowering stalk (brassica campestris l. ssp. chinensis var. purpurea hort.) seedlings.the aim of this research was to investigate how exogenous abscisic acid (aba) alleviates cadmium (cd) toxicity in purple flowering stalk (brassica campestris l. ssp. chinensis) and evaluate whether it could be a potential choice for phytoremediation. purple flowering stalk seedlings were cultivated in a hydroponic system with cd at various concentrations (0-100 μmol l-1) as controls and cd plus aba as the treatment in the growth media. the soluble proteins, chlorophyll contents and the activity ...201728755629
improvement of pig manure compost lignocellulose degradation, organic matter humification and compost quality with medical stone.the present study aimed to investigate the effect of different concentrations (0%, 2.5%, 5.0%, 7.5% and 10.0%) of medical stone (ms) on the lignocellulose degradation and organic matter humification during pig manure (pm) composting. the results indicated that the addition of ms drastically promoted the organic carbon and lignin degradation. compared to the control, the decomposition rate of hemicellulose and cellulose was increased by 9.64-27.08% and 2.11-12.07% in ms added treatments. meanwhil ...201728711806
effects of an iron-silicon material, a synthetic zeolite and an alkaline clay on vegetable uptake of as and cd from a polluted agricultural soil and proposed remediation mechanisms.economic and highly effective methods of in situ remediation of cd and as polluted farmland in mining areas are urgently needed. pot experiments with brassica chinensis l. were carried out to determine the effects of three soil amendments [a novel iron-silicon material (ism), a synthetic zeolite (sz) and an alkaline clay (ac)] on vegetable uptake of as and cd. sem-eds and xrd analyses were used to investigate the remediation mechanisms involved. amendment with ism significantly reduced the conce ...201727530933
transcriptomic analysis of pak choi under acute ozone exposure revealed regulatory mechanism against ozone stress.ground-level ozone (o3) is one of the major air pollutants, which cause oxidative injury to plants. the physiological and biochemical mechanisms underlying the responses of plants to o3 stress have been well investigated. however, there are limited reports about the molecular basis of plant responses to o3. in this study, a comparative transcriptomic analysis of pak choi (brassica campestris ssp. chinensis) exposed to different o3 concentrations was conducted for the first time.201729216819
identification and fine mapping of a stay-green gene (brnye1) in pakchoi (brassica campestris l. ssp. chinensis).using bulked segregant analysis combined with next-generation sequencing, we delimited the brnye1 gene responsible for the stay-green trait of nye in pakchoi. sequence analysis identified bra019346 as the candidate gene. "stay-green" refers to a plant trait whereby leaves remain green during senescence. this trait is useful in the cultivation of pakchoi (brassica campestris l. ssp. chinensis), which is marketed as a green leaf product. this study aimed to identify the gene responsible for the st ...201729209732
subcellular distribution and biotransformation of phenanthrene in pakchoi after inoculation with endophytic pseudomonas sp. as probed using hrms coupled with isotope-labeling.contamination of food-crops with polycyclic aromatic hydrocarbons (pahs) poses a grave concern to food safety, especially when pahs are internalized. we have demonstrated in our previous study that inoculation of crop with a phenanthrene-degrading endophytic pseudomonas sp. ph6-gfp could overcome this problem. here, the subcellular distribution and biotransformation mechanism of phenanthrene in pakchoi (brassica chinensis l.) seedlings with inoculation of ph6-gfp were further investigated both i ...201729150254
field crops (ipomoea aquatica forsk. and brassica chinensis l.) for phytoremediation of cadmium and nitrate co-contaminated soils via rotation with sedum alfredii hance.phytoremediation coupled with crop rotation (pcc) is a feasible strategy for remediation of contaminated soil without interrupting crop production. the objective of this study was to develop a pcc technology system for greenhouse fields co-contaminated with cd and nitrate using hyperaccumulator sedum alfredii. in this system, endophytic bacterium m002 inoculation, co2 fertilization, and fermentation residue were continuously applied to improve the growth of s. alfredii, and low-accumulator ipomo ...201728669090
pulsed light-emitting diodes for a higher phytochemical level in microgreens.a novel research of pulsed light-emitting diode (led) lighting versus continuous lighting was conducted by analyzing phytochemical levels in microgreens. red pak choi (brassica rapa var. chinensis), mustard (brassica juncea l.), and tatsoi (brassica rapa var. rosularis) were grown indoors under hps lamps supplemented with monochromatic (455, 470, 505, 590, and 627 nm) leds [total photosynthetic photon flux density (ppfd) of 200 ± 10 μmol m-2 s-1, for 16 h day-1]. for pulsed light treatments, the ...201728618783
characterization of bcmf23a and bcmf23b, two putative pectin methylesterase genes related to pollen development in brassica campestris ssp. chinensis.two homologous genes, brassica campestris male fertility 23a (bcmf23a) and brassica campestris male fertility 23b (bcmf23b), encoding putative pectin methylesterases (pmes) were isolated from brassica campestris ssp. chinensis (syn. brassica rapa ssp. chinensis). these two genes sharing high sequence identity with each other were highly expressed in the fertile flower buds but silenced in the sterile ones of genic male sterile line system ('bcajh97-01a/b'). results of rt-pcr and in situ hybridiz ...201727844189
[methods of bio-engineering destruction of root residues in application to a space vitamin greenhouse with ionite artificial soil].regeneration of ionite artificial soil (as) in root modules (rm) of conveyor-type space greenhouses should be proceeded by removal of root residues without disruption of the as capillary-porous structure. the proposed method consists of two stages of root residues destruction. on the first stage, as treatment by 0.7% an alkali water solution with added 0.7% hydrogen peroxide over 3.5 hours in a thermal-insulation container at 95 +/- 4 degrees c reduces root residue mass up to 60%. specific energ ...201723116044
[rna-dependent rna-polymerase in brassica chinensis l]. 20164987649
analysis of genetic diversity of brassica rapa var. chinensis using issr markers and development of scar marker specific for fragrant bok choy, a product of geographic indication.non-heading chinese cabbage [brassica rapa var. chinensis (linnaeus) kitamura] is a popular vegetable and is also used as a medicinal plant in traditional chinese medicine. fragrant bok choy is a unique accession of non-heading chinese cabbage and a product of geographic indication certified by the ministry of agriculture of china, which is noted for its rich aromatic flavor. however, transitional and overlapping morphological traits can make it difficult to distinguish this accession from other ...201627173238
effects of dissolved organic matter on uptake and translocation of lead in brassica chinensis and potential health risk of pb.dissolved organic matter (dom) can affect the bioavailability of heavy metals in soil, especially in soils used for vegetable production, where intensive organic fertilization is applied. the present study examined the effects of dom derived from commercial organic fertilizers (cof), cow manure (com) and chicken manure (chm), on uptake and translocation of lead (pb) in brassica chinensis in a pot experiment. the results indicate that dom derived from chm (domchm) significantly increased pb conce ...201627399753
accumulation of cr, cd, pb, cu, and zn by plants in tanning sludge storage sites: opportunities for contamination bioindication and phytoremediation.tanning sludge enriched with high concentrations of cr and other metals has adverse effects on the environment. plants growing in the metalliferous soils may have the ability to cope with high metal concentrations. this study focuses on potentials of using native plants for bioindication and/or phytoremediation of cr-contaminated sites. in the study, we characterized plants and soils from six tanning sludge storage sites. soil in these sites exhibited toxic levels of cr (averaged 16,492 mg kg(-1 ...201627552994
biochar reduces the bioavailability of di-(2-ethylhexyl) phthalate in soil.a pot experiment was conducted to evaluate the effect of biochars on the bioavailability of di-(2-ethylhexyl) phthalate (dehp) in two soils using brassica chinensis l. as an indicator plant. the residual concentrations of dehp tended to be higher in the biochar-amended soils than in the control soils. they were lower (p<0.05) in the high organic carbon content soil (hoc; 2.2%c) than in the low organic carbon content soil (loc; 0.35%c). the dehp concentrations in plant shoots grown in the hoc soi ...201626037111
light intensity affects the uptake and metabolism of glycine by pakchoi (brassica chinensis l.).the uptake of glycine by pakchoi (brassica chinensis l.), when supplied as single n-source or in a mixture of glycine and inorganic n, was studied at different light intensities under sterile conditions. at the optimal intensity (414 μmol m(-2) s(-1)) for plant growth, glycine, nitrate, and ammonium contributed 29.4%, 39.5%, and 31.1% shoot n, respectively, and light intensity altered the preferential absorption of n sources. the lower (15)n-nitrate in root but higher in shoot and the higher (15 ...201626882864
attempts to improve nitrogen utilization efficiency of aquaponics through nitrifies addition and filler gradation.aquaponics has attracted worldwide attention in recent years and is considered as an alternative technology for conventional aquaculture. in this study, common carp (cyprinus carpio) and pakchoi (brassica chinensis) were cultured in lab-scale aquaponics, and attempts were conducted to enhance its nitrogen utilization efficiency (nue) through two optimization methods, i.e., nitrifies addition (na) and filler gradation (fg). results showed that na and fg could improve the nue of aquaponics by 8.8 ...201626645232
effects of aging on the fraction distribution and bioavailability of selenium in three different soils.aging refers to the processes by which the mobility and bioavailability of metals in soil decline with time. although long-term aging is a key process that needs to be considered in risk assessment of metals, few investigations has been attempted to determine whether and how residence time influences the selenium (se) fractions and bioavailability in soil. in this study, the fractions of se in soils was evaluated, and bioavailability were assessed by measuring se concentration in pak choi (brass ...201626606190
sulfur decreases cadmium translocation and enhances cadmium tolerance by promoting sulfur assimilation and glutathione metabolism in brassica chinensis l.we investigated the ameliorative role of sulfur (s) in protecting plants against cadmium (cd) toxicity by using two pakchoi (brassica chinensis l.) cultivars with different cd tolerance levels. the exposure of pakchoi seedlings to 100μm cd inhibited plant growth, increased superoxide content, enhanced membrane lipid peroxidation, and induced cd accumulation in the roots and shoots. application of s to cd-stressed plants alleviated cd-induced oxidative stress by promoting the capacity of the asco ...201626513528
effect of kelp waste extracts on the growth and development of pakchoi (brassica chinensis l.).to explore the effects of kelp waste extracts (kwe) on the growth and development of brassia chinensis l., germination and greenhouse experiments were carried out under different concentrations of kwe. the results showed that a higher germination percentage (95%), associated with high germination index (8.70), germination energy (71.67%) and seedling vigor index (734.67), was obtained under a lower kwe concentration (2%) compared with the control. the radicle length (4.97 cm), fresh weight (0.32 ...201627934911
toxicity of combined chromium(vi) and phenanthrene pollution on the seed germination, stem lengths, and fresh weights of higher plants.studies of the interaction and toxicity of pollutant combinations such as heavy metals and pahs are of practical importance in the remediation and monitoring of the industrial soil environment. this study investigated the single and combined toxicity of chromium(vi) and phenanthrene on three important higher plants: mung beans (phaseolus aureus), pakchoi cabbage (brassica chinensis), and rice (oryza sativa). in experiments using artificial soil matrix, the ec10 and ec20 of the two pollutants, al ...201627098980
comparative transcriptome analysis between low- and high-cadmium-accumulating genotypes of pakchoi (brassica chinensis l.) in response to cadmium stress.to reduce cadmium (cd) pollution of food chains, screening and breeding of low-cd-accumulating cultivars are the focus of much study. two previously identified genotypes, a low-cd-accumulating genotype (lajk) and a high-cd-accumulating genotype (hajs) of pakchoi (brassica chinesis l.), were stressed by cd (12.5 μm) for 0 h (t0), 3 h (t3) and 24 h (t24). by comparative transcriptome analysis for root tissue, 3005 and 4343 differentially expressed genes (degs) were identified in lajk at t3 (vs t0) ...201627228483
effects of selenite and selenate application on growth and shoot selenium accumulation of pak choi (brassica chinensis l.) during successive planting conditions.selenate and selenite are two main kinds of inorganic selenium (se) sources in soil, but these substances can pose threats to the environment. phytoextraction is an emerging technology to remove se from polluted soils by using a hyper-accumulator. in this study, a pot experiment was conducted to investigate se phytoextraction potential of pak choi (brassica chinensis l.) and to determine the effects of se on growth and se accumulation of pak choi under successive planting conditions (four crops) ...201525794583
accumulation, allocation, and risk assessment of polycyclic aromatic hydrocarbons (pahs) in soil-brassica chinensis system.farmland soil and leafy vegetables accumulate more polycyclic aromatic hydrocarbons (pahs) in suburban sites. in this study, 13 sampling areas were selected from vegetable fields in the outskirts of xi'an, the largest city in northwestern china. the similarity of pah composition in soil and vegetation was investigated through principal components analysis and redundancy analysis (rda), rather than discrimination of pah congeners from various sources. the toxic equivalent quantity of pahs in soil ...201525679782
photocatalytic degradation of acephate in pak choi, brassica chinensis, with ce-doped tio2.the photocatalytic degradation of acephate was investigated using ce-doped tio2 (tio2/ce) hydrosol. in contrast to previous research conducted under artificial light in the laboratory, this study investigated the decomposition of acephate in a field trial. the results show that acephate can be efficiently degraded by the tio2/ce system under natural field conditions; the degradation efficiency was affected by the dosage of the photocatalyst and acephate. the optimum dosage of tio2/ce was 2400 g ...201525826101
interplay between orfamides, sessilins and phenazines in the control of rhizoctonia diseases by pseudomonas sp. cmr12a.we investigated the role of phenazines and cyclic lipopeptides (clps) (orfamides and sessilins), antagonistic metabolites produced by pseudomonas sp. cmr12a, in the biological control of damping-off disease on chinese cabbage (brassica chinensis) caused by rhizoctonia solani ag 2-1 and root rot disease on bean (phaseolus vulgaris l.) caused by r. solani ag 4-hgi. a pseudomonas mutant that only produced phenazines suppressed damping-off disease on chinese cabbage to the same extent as cmr12a, whi ...201526085277
molecular cloning and expression analysis of a cu/zn sod gene (bccsd1) from brassica campestris ssp. chinensis.superoxide dismutases (sods) are a family of metalloproteins extensively exists in eukaryote, which plays an essential role in stress-tolerance of higher plants. a full-length cdna encoding cu/zn sod (bccsd1) was isolated from young seedlings of non-heading chinese cabbage (brassica campestris ssp. chinensis) by rapid amplification of cdna ends (race). bioinformatics analysis revealed that bccsd1 belonged to the plant sod super family and had the closest relationship with sod from brassica napus ...201525976826
genome-wide identification of turnip mosaic virus-responsive micrornas in non-heading chinese cabbage by high-throughput sequencing.turnip mosaic virus (tumv) is the most prevalent viral pathogen infecting most cruciferous plants. micrornas (mirnas) are around 22 nucleotides long non-protein-coding rnas that play key regulatory roles in plants. recent research findings show that mirnas are involved in plant-virus interaction. however we know little about plant defense and viral offense system networks throughout microrna regulation pathway. in this study, two small rna libraries were constructed based on non-heading chinese ...201526115771
[identification of soft rot pathogens on chinese cabbage [brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee] in beijing].this study aimed to identify soft rot pathogens of chinese cabbage [brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee] in beijing.201526939453
assessment of heavy metal pollution in vegetables and relationships with soil heavy metal distribution in zhejiang province, china.there are increasing concerns on heavy metal contaminant in soils and vegetables. in this study, we investigated heavy metal pollution in vegetables and the corresponding soils in the main vegetable production regions of zhejiang province, china. a total of 97 vegetable samples and 202 agricultural soil samples were analyzed for the concentrations of cd, pb, as, hg, and cr. the average levels of cd, pb, and cr in vegetable samples [chinese cabbage (brassica campestris spp. pekinensis), pakchoi ( ...201526013654
use of monoclonal antibodies in an elisa for detecting an invasive pest insect, liriomyza trifolii (diptera: agromyzidae).a monoclonal antibody was prepared by the hybridoma technology. it reacted only with the protein of liriomyza trifolii (burgess) and not with that of chromatomyia horticola goureau or liriomyza sativae blanchard in indirect enzyme-linked immunosorbent assay. it was effective even after being diluted more than 8.192×10(6)-fold. the detection sensitivity of the antibody was 31.3 µg/ml under controlled conditions. positive reaction was achieved with all laboratory-reared l. trifolii samples, includ ...201526470159
effects of inorganic and organic amendments on the uptake of lead and trace elements by brassica chinensis grown in an acidic red soil.a greenhouse study was conducted to investigate the effects of inorganic (phosphate rock, single superphosphate and calcium magnesium phosphate) and organic amendments (peat, straw manure and pig manure) on the uptake of lead (pb) and trace elements by chinese cabbage (brassica chinensis) grown in an acidic red soil. the application of all organic amendments increased the soil ph while inorganic amendments such as single superphosphate did not. both inorganic and organic amendments decreased the ...201524992219
directional transfer of a multiple-allele male sterile line in brassica campestris l. ssp. chinensis (l.) makino var. rosularis tsen et lee.to produce hybrid seeds of wutacai (brassica campestris l. ssp. chinensis (l.) makino var. rosularis tsen et lee), a "directional transfer program" was designed to breed the multiple-allele male sterile line of wutacai. a multiple-allele male sterile line of naibaicai (brassica campestris l. ssp. chinensis l., s01) was used as the male sterile resource, and an inbred line of wutacai (wt01) was used as the target line. recurrent backcrossing was employed to transfer the male sterility and other b ...201424987301
identification of novel and conserved mirnas involved in pollen development in brassica campestris ssp. chinensis by high-throughput sequencing and degradome analysis.micrornas (mirnas) are endogenous, noncoding, small rnas that have essential regulatory functions in plant growth, development, and stress response processes. however, limited information is available about their functions in sexual reproduction of flowering plants. pollen development is an important process in the life cycle of a flowering plant and is a major factor that affects the yield and quality of crop seeds.201424559317
petroleum contamination of soil and water, and their effects on vegetables by statistically analyzing entire data set.aliphatic hydrocarbons have been used to assess total oil concentrations, petroleum sources, and petroleum degradation. in this study, surface soil, groundwater, surface water, and vegetables were collected from the outskirts of xi'an, the largest city in northwestern china, and the samples were analyzed for aliphatic hydrocarbon contents. the concentrations of n-alkanes were 1.06-4.01 μg/g in the soil. the concentrations and the geochemical characteristics of n-alkanes showed that the low carbo ...201424468500
exposure factors for wastewater-irrigated asian vegetables and a probabilistic rotavirus disease burden model for their consumption.many farmers in water-scarce regions of developing countries use wastewater to irrigate vegetables and other agricultural crops, a practice that may expand with climate change. there are a number of health risks associated with wastewater irrigation for human food crops, particularly with surface irrigation techniques common in the developing world. the world health organization (who) recommends using quantitative microbial risk assessment (qmra) to determine if the irrigation scheme meets healt ...201424576153
anthocyanin accumulation and transcriptional regulation of anthocyanin biosynthesis in purple bok choy (brassica rapa var. chinensis).bok choy (brassica rapa var. chinensis) is an important dietary vegetable cultivated and consumed worldwide for its edible leaves. the purple cultivars rich in health-promoting anthocyanins are usually more eye-catching and valuable. fifteen kinds of anthocyanins were separated and identified from a purple bok choy cultivar (zi he) by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. to investigate the molecular mechanisms underlying anthocyanin accumulatio ...201425419600
pectate lyase-like10 is associated with pollen wall development in brassica campestris.pectate lyase-like10 (pll10) was previously identified as one of the differentially expressed genes both in microspores during the late pollen developmental stages and in pistils during the fertilization process in chinese cabbage (brassica campestris ssp. chinensis). here, antisense-rna was used to study the functions of bcpll10 in chinese cabbage. abnormal pollen was identified in the transgenic lines (bcpll10-4, -5, and -6). in fertilization experiments, fewer seeds were harvested when the an ...201424773757
proteomic analysis of non-heading chinese cabbage infected with hyaloperonospora parasitica.downy mildew is a serious fungal disease in non-heading chinese cabbage (brassica campestris ssp. chinensis makino) that is caused by hyaloperonospora parasitica, which infects members of the brassicaceae family. for breeding improvement, researchers must understand the defence mechanisms employed by non-heading chinese cabbage to combat h. parasitica infection. using 2-de protein analysis, we compared the proteomes from leaves of non-heading chinese cabbage seedlings that were infected with h. ...201424334100
variations in the natural ¹⁵n abundance of brassica chinensis grown in uncultivated soil affected by different nitrogen fertilizers.to further investigate the method of using δ(15)n as a marker for organic vegetable discrimination, the effects of different fertilizers on the δ(15)n in different growing stages of brassica chinensis (b. chinensis) grown in uncultivated soil were investigated with a pot experiment. b. chinensis was planted with uncultivated soil and different fertilizer treatments and then harvested three times in three seasons consecutively. for the spring experiments in the years of 2011 and 2012, the δ(15)n ...201425369912
bcpmi2, isolated from non-heading chinese cabbage encoding phosphomannose isomerase, improves stress tolerance in transgenic tobacco.phosphomannose isomerase (pmi) is an enzyme that catalyses the first step of the l-galactose pathway for ascorbic acid (asa) biosynthesis in plants. to clarify the physiological roles of pmi in asa biosynthesis, the cdna sequence of pmi was cloned from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) and overexpressed in tobacco transformed with agrobacterium tumefaciens. the asa and soluble sugar contents were lower in 35s::bcpmi2 tobacco than in wild-type tobacco. howeve ...201424430300
bioactive metabolites isolated from penicillium sp. yy-20, the endophytic fungus from ginkgo biloba.six known metabolites, adenosine (1), methyl β-d-ribofuranoside (2), adenine (3), 2'-deoxyadenosine (4), 3-methylpiperazine-2,5-dione (5) and 2'-deoxyuridine (6), were isolated from the extracts of the endophytic fungus penicillium sp. yy-20 isolated from the root of ginkgo biloba, and their structures were elucidated by spectroscopic methods. the antioxidant and growth-promoting activities of these compounds were first evaluated. the results indicated that compounds 1, 3 and 4 exhibited potenti ...201424144081
persistence and dissipation of chlorpyrifos in brassica chinensis, lettuce, celery, asparagus lettuce, eggplant, and pepper in a greenhouse.the residue behavior of chlorpyrifos, which is one of the extensively used insecticides all around the world, in six vegetable crops was assessed under greenhouse conditions. each of the vegetables was subjected to a foliar treatment with chlorpyrifos. two analytical methods were developed using gas chromatography equipped with a micro-ecd detector (loq = 0.05 mg kg(-1)) and liquid chromatography with a tandem mass spectrometry (loq = 0.01 mg kg(-1)). the initial foliar deposited concentration o ...201424967589
phytoavailability of cadmium (cd) to pak choi (brassica chinensis l.) grown in chinese soils: a model to evaluate the impact of soil cd pollution on potential dietary toxicity.food chain contamination by soil cadmium (cd) through vegetable consumption poses a threat to human health. therefore, an understanding is needed on the relationship between the phytoavailability of cd in soils and its uptake in edible tissues of vegetables. the purpose of this study was to establish soil cd thresholds of representative chinese soils based on dietary toxicity to humans and develop a model to evaluate the phytoavailability of cd to pak choi (brassica chinensis l.) based on soil p ...201425386790
identification of differentially accumulating pistil proteins associated with self-incompatibility of non-heading chinese cabbage.non-heading chinese cabbage (brassica campestris l. ssp. chinensis makino), an important vegetable crop in china, exhibits a typical sporophytic self-incompatibility (si) system. to better understand the mechanism of si response and identify potential candidate proteins involved in the si system of this vegetable crop, the proteomic approach was taken to identify differential accumulating pistil proteins. pistils were collected at 0 h and 2 h after self-pollination at anthesis in self-incompatib ...201423581423
evaluation of phytoavailability of heavy metals to chinese cabbage (brassica chinensis l.) in rural soils.this study compared the extractability of cd, cu, ni, pb, and zn by 8 extraction protocols for 22 representative rural soils in taiwan and correlated the extractable amounts of the metals with their uptake by chinese cabbage for developing an empirical model to predict metal phytoavailability based on soil properties. chemical agents in these protocols included dilute acids, neutral salts, and chelating agents, in addition to water and the rhizon soil solution sampler. the highest concentrations ...201425295297
the effectiveness of spent coffee grounds and its biochar on the amelioration of heavy metals-contaminated water and soil using chemical and biological assessments.spent coffee grounds (scg) and charred spent coffee grounds (scg-char) have been widely used to adsorb or to amend heavy metals that contaminate water or soil and their success is usually assessed by chemical analysis. in this work, the effects of scg and scg-char on metal-contaminated water and soil were evaluated using chemical and biological assessments; a phytotoxicity test using bok choy (brassica campestris l. ssp. chinensis jusl.) was conducted for the biological assessment. when scg and ...201425242543
transformation of heavy metal fractions on soil urease and nitrate reductase activities in copper and selenium co-contaminated soil.this study aims to explore the effects of the distribution, transformation and bioavailability of different fractions of copper (cu) and selenium (se) in co-contaminated soils on soil enzymes, providing references for the phytoremediation of contaminated areas and agriculture environmental protection. pot experiments and laboratory analysis were used to investigate the transformation and bioavailability of additional cu and se for pakchoi (brassica chinensis) in co-contaminated soil. in the unco ...201425193883
expression analysis of self-incompatibility-associated genes in non-heading chinese cabbage.in brassicaceae, a self-incompatibility (si) system mediates pollen-pistil interactions. self-pollen could be recognized and rejected by incompatible pistils. several components involved in the si response have been determined, including s-locus receptor kinase (srk), s-locus cysteine-rich protein/s-locus protein 11, and arm repeat-containing protein 1 (arc1). however, the components involved in the si system of brassicaceae are not fully understood. here, we detected expression patterns of 24 s ...201425062491
molecular cloning and expression of the male sterility-related ctyabby1 gene in flowering chinese cabbage (brassica campestris l. ssp chinensis var. parachinensis).expression of the yabby gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. however, the yabby gene has not been studied in male sterility and fertility restoration. we homologously cloned the ctyabby1 gene of male-sterile tc1 in brassica campestris l. ssp chinensis var. parachinensis; its expression was analyzed by real-time pcr. a 93 ...201425036178
effects of spray application of lanthanum and cerium on yield and quality of chinese cabbage (brassica chinensis l) based on different seasons.the use of single light rare earth elements in agriculture was studied using the application of single rare earth elements (la and ce) to chinese cabbage (brassica chinensis l.) in field plot experiments where the soil conditions remained the same but the season (spring or autumn) in which application occurred was altered. the results showed that the chinese cabbage's nutrition quality between the two seasons had obvious differences. when planted in the autumn, the soluble sugar and vitamin c co ...201425022243
bcmf21 is important for pollen development and germination in brassica campestris ssp. chinensis.brassica campestris male fertility 21 (bcmf21) was previously isolated from the flower buds of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) and expressed specifically in tapetum and microspores during the meiosis stage and the uninucleate stage of microspore development. here, we used antisense rna technology to knock down the expression level of bcmf21 in b. campestris and analyzed the phenotype of the transgenic plants. alexander staining and scan ...201424323195
evaluation of selenium species in selenium-enriched pakchoi (brassica chinensis jusl var parachinensis (bailey) tsen & lee) using mixed ion-pair reversed phase hplc-icp-ms.hplc-icp-ms based on ion-paired reversed phase chromatography for the selenium speciation using the mixture of 1-butanesulfonic acid (ba) and trifluoroacetic acid (tfa) as the mixed ion-pairing reagents was developed and applied to selenium-enriched pakchoi (brassica chinensis jusl var parachinensis (bailey) tsen & lee). several conditions of ion-paired reversed phase hplc-icp-ms, such as ph of the mobile phase, concentration of ion pairing reagents, types and length of analytical column, and fl ...201424128538
antioxidant enzymes regulate reactive oxygen species during pod elongation in pisum sativum and brassica chinensis.previous research has focused on the involvement of reactive oxygen species (ros) in cell wall loosening and cell extension in plant vegetative growth, but few studies have investigated ros functions specifically in plant reproductive organs. in this study, ros levels and antioxidant enzyme activities were assessed in pisum sativum and brassica chinensis pods at five developmental stages. in juvenile pods, the high levels of o2.- and .oh indicates that they had functions in cell wall loosening a ...201424503564
effects of zno nanoparticles and microwave heating on the sterilization and product quality of vacuum-packaged caixin.the sterilization of vacuum-packaged caixin (brassica chinensis l.), which is a green-leafy vegetable and also a low-acid food, remains a difficult problem. in this study, effects of zno nanoparticles and microwave heating on the sterilization and product quality of vacuum-packaged caixin were investigated.201424464779
proteomic analysis of pakchoi leaves and roots under glycine-nitrogen conditions.the physiological and differential proteomic responses of pakchoi leaves and roots to glycine-nitrogen (gly-n) treatments were determined. two pakchoi (brassica campestris ssp. chinensis l. makino. var. communis tsen et lee) cultivars, 'huawang' and 'wuyueman', were grown under sterile hydroponic conditions with different n forms (gly-n and nitrate-n). gly-n-treated pakchoi exhibited decreased fresh weights, total n uptake, leaf areas, and net photosynthetic rates than those treated with nitrate ...201424429133
[differences of selenium uptake pattern of pakchoi and the possible mechanism when amended with selenate and selenite].in order to produce selenium-enriched crops and provide a theoretical basis of phytoremediation to selenium contaminated soil, pot experiment and lab analysis were carried out to examine the differences of selenium uptake pattern and bioavailability to pakchoi (brassica chinensis l.) when amended with selenate and selenite. meanwhile, dynamic changes of soil available se was analyzed to explore the reason. the results showed that no significant differences in pakchoi shoot biomass between selena ...201324191579
comparison of ascorbic acid biosynthesis in different tissues of three non-heading chinese cabbage cultivars.ascorbic acid (l-asa) is an important antioxidant in plants and humans. vegetables are one of the main sources of ascorbic acid for humans. for instance, non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is considered as one of the most important vegetables in south china. to elucidate the mechanism by which asa accumulates, we systematically investigated the expression profiles of d-mannose/l-galactose pathway-related genes. we also investigated the recycling-related genes ...201324157701
identification of gene expression profile during fertilization in brassica campestris subsp. chinensis.fertilization is controlled by a complex gene regulatory network. to study the fertilization mechanism, we determined time courses of the four developmental stages of fertilization in chinese cabbage pak-choi (brassica campestris subsp. chinensis) by cytological observation. we then used the arabidopsis ath1 microarray to characterize the gene expression profiles of pollinated and unpollinated pistils in b. campestris subsp. chinensis. the result showed 44 up-regulated genes and 33 down-regulate ...201323379337
the dynamic growth exhibition and accumulation of cadmium of pak choi (brassica campestris l. ssp. chinensis) grown in contaminated soils.the accumulation of heavy metals, especially cadmium (cd), in leafy vegetables was compared with other vegetables. pak choi (brassica campestris l. ssp. chinensis) is a leafy vegetable consumed in taiwan and its safety for consumption after growing in contaminated soils is a public concern. a pot experiment (50 days) was conducted to understand the dynamic accumulation of cd by pak choi grown in artificially contaminated soils. the edible parts of pak choi were sampled and analyzed every 2-3 day ...201324284350
characterization and co-expression analysis of wrky orthologs involved in responses to multiple abiotic stresses in pak-choi (brassica campestris ssp. chinensis).the wrky transcription factor is an important member of the stress-related transcription factors, which mediate diverse abiotic stresses in many plants. however, up until now, the number of wrky members, and the regulatory mechanisms involved in abiotic stress responses in pak-choi (brassica campestris ssp. chinensis), remained unknown.201324267479
rape (brassica chinensis l.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis(2-ethylhexyl) phthalate.phthalic acid esters (paes) pollution in agricultural soils caused by widely employed plastic products is becoming more and more widespread in china. paes polluted soil can lead to phytotoxicity in higher plants and potential health risks to human being. we evaluated the individual toxicity of di-n-butyl phthalate (dnbp) and bis(2-ethylhexyl) phthalate (dehp), two representative paes, to sown rape (brassica chinensis l.) seeds within 72 h (as germination stage) and seedlings after germination fo ...201323389857
organic nitrogen components in soils from southeast china.to investigate the amounts of extractable organic nitrogen (eon), and the relationships between eon and total extractable nitrogen (ten), especially the amino acids (aas) adsorbed by soils, and a series of other hydrolyzed soil nitrogen indices in typical land use soil types from southeast china. under traditional agricultural planting conditions, the functions of eon, especially aas in the rhizosphere and in bulk soil zones were also investigated.201323549843
chemical stabilization of cadmium in acidic soil using alkaline agronomic and industrial by-products.in situ immobilization of heavy metals using reactive or stabilizing materials is a promising solution for soil remediation. therefore, four agronomic and industrial by-products [wood biochar (wb), crushed oyster shell (os), blast furnace slag (bfs), and fluidized-bed crystallized calcium (fbcc)] and caco3 were added to acidic soil (cd = 8.71 mg kg(-1)) at the rates of 1%, 2%, and 4% and incubated for 90 d. chinese cabbage (brassica chinensis l.) was then planted in the soil to test the cd uptak ...201323947715
identification of micrornas potentially involved in male sterility of brassica campestris ssp. chinensis using microrna array and quantitative rt-pcr assays.micrornas (mirnas) are a class of newly identified, noncoding, small rna molecules that negatively regulate gene expression. many mirnas are reportedly involved in plant growth, development and stress response processes. however, their roles in the sexual reproduction mechanisms in flowering plants remain unknown. pollen development is an important process in the life cycle of a flowering plant, and it is closely related to the yield and quality of crop seeds. this study aimed to identify mirnas ...201323864334
subcellular distribution of metals within brassica chinensis l. in response to elevated lead and chromium stress.differential centrifugation and synchrotron radiation x-ray fluorescence spectroscopy (srxrf) microprobe were used to study the distribution of the elements in tissue cross sections of pakchoi ( brassica chinensis l.) under stress of elevated pb and cr. subcellular fractionation of the different tissues grown in a nutrient solution containing 200 mg l(-1) pb or 5 mg l(-1) cr showed that 86.7 and 76.3% of the pb that accumulated in the roots and shoots, respectively, was contained in the cell wal ...201323621278
reducing the bioavailability of pcbs in soil to plant by biochars assessed with triolein-embedded cellulose acetate membrane technique.coupling with triolein-embedded cellulose acetate membrane (tecam) technique, hydroxypropyl β-cyclodextrins (hpcd) extraction method, and the greenhouse pot experiments, the influences of biochars on polychlorinated biphenyls (pcbs) bioavailability in soil to plant (brassica chinensis l. and daucus carota) were investigated. addition of 2% biochars to soils significantly reduced the uptake of pcbs in plant, especially for di-, tri- and tetra-chlorobiphenyls. pcbs concentrations in the roots of b ...201323279904
uptake of di(2-ethylhexyl) phthalate (dehp) by the plant benincasa hispida and its use for lowering dehp content of intercropped vegetables.uptake of di(2-ethylhexyl) phthalate (dehp) by the plant benincasa hispida and its use for topical phytoremediation were investigated by cultivation of plants in dehp-contaminated environments. the results showed that major plant organs of b. hispida , including leaves, stems, and fruits, readily absorbed dehp from the air. the amount of dehp that accumulated in leaves, stems, and fruits was mainly dependent upon exposure time, and most dehp accumulated in their inner tissues. a single plant of ...201323675826
changes in the relative abundance and movement of insect pollinators during the flowering cycle of brassica rapa crops: implications for gene flow.the potential movement of transgenes from genetically modified crops to non-genetically modified crops via insect-mediated pollen dispersal has been highlighted as one of the areas of greatest concern in regards to genetically modified crops. pollen movement depends sensitively on spatial and temporal variation in the movement of insect pollinators between crop fields. this study tested the degree of variation in the diversity and relative abundance of flower-visiting insects entering versus lea ...201323937538
effect of modified atmosphere packaging on the quality and bioactive compounds of chinese cabbage (brasicca rapa l. ssp. chinensis).the perishability of brassica chinensis poses a major challenge to distribution and marketing. the aim of this work was to select a suitable modified atmosphere packaging to retain the overall quality and bioactive compounds during storage.201323254451
bcmf11, a novel non-coding rna gene from brassica campestris, is required for pollen development and male fertility.key message : bcmf11 as a non-coding rna gene has an essential role in pollen development, and might be useful for regulating the pollen fertility of crops by antisense rna technology. we previously identified a 828-bp full-length cdna of bcmf11, a novel pollen-specific non-coding mrna-like gene from chinese cabbage (brassica campestris l. ssp. chinensis makino). however, little information is known about the function of bcmf11 in pollen development. to investigate its exact biological roles in ...201323064614
microtubule and male sterility in a gene-cytoplasmic male sterile line of non-heading chinese cabbage.microtubules are the basic components of the cytoskeleton in eukaryotic cells and are made up of 13 parallel protofilaments, each composed of α- and β-tubulin unit molecules aligned along the longitudinal axis of the microtubule.201222581783
sequence characterization and expression pattern of bcmf21, a novel gene related to pollen development in brassica campestris ssp. chinensis.in this report a full length cdna, brassica campestris male fertile 21 (bcmf21) was successfully isolated from one of the cdna-amplified fragment length polymorphism (cdna-aflp) transcript-derived fragments (tdfs), bbp10, that was found down-regulated in the flower buds of sterile plants in brassica campestris l. ssp. chinensis makino genic male sterile (gms) ab line system (bcajh 97-01a/b). bcmf21 protein structure analysis showed a signal peptide at the n-terminus; two protein kinase c phospho ...201222311045
effect of some plant growth regulators on lindane and alpha-endosulfan toxicity to brassica chinensis.the effect of indolebutyric acid (iba) and gibberellic acid (ga3), to alleviate the organochlorine phytotoxicity were studied in brassica chinensis. presence of organochlorine decreased brassica chinensis seedlings growth in contaminated alkaline soil. one mg l(-1) iba could enhance 14 and 26% shoot and root length of b. chinensis seedlings grown at 40 mg kg(-1) lindane contaminated soil, respectively. ten mg l(-1) iba also increased 80 and 40% root fresh weight of seedling grown in 40 mg kg(-1) ...201223360012
evaluation of iodide and iodate for adsorption-desorption characteristics and bioavailability in three types of soil.adsorption-desorption of iodine in two forms, viz., iodide (i(-)) and iodate (io (3) (-) ), in three types of soil were investigated. the soils were: red soil developed on quaternary red earths (req)- clayey, kaolintic thermic plinthite aquult, inceptisol soil (is) and alluvial soil (as)-fluvio-marine yellow loamy soil. the isothermal curves of iodine adsorption on soils were described by langmuir and freundlich equation, and the maximum adsorption values (y (m)) were obtained from the simple la ...201222038267
permissible value for vanadium in allitic udic ferrisols based on physiological responses of green chinese cabbage and soil microbes.greenhouse experiments were conducted to study the permissible value of vanadium (v) based on the growth and physiological responses of green chinese cabbage (brassica chinensis l.), and effects of v on microbial biomass carbon (mbc) and enzyme activities in allitic udic ferrisols were also studied. the results showed that biomass of cabbage grown on soil treated with 133 mg v kg(-1) significantly decreased by 25.1% compared with the control (p < 0.05). vanadium concentrations in leaves and root ...201221898106
bcnrt1, a plasma membrane-localized nitrate transporter from non-heading chinese cabbage.a nitrate transporter, bcnrt1, was isolated from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) cultivar 'suzhouqing'. the full-length cdna was obtained using the rapid amplification of cdna ends technique and contains an open reading frame of 1,770 bp that predicts a protein of 589 acid residues that possesses 12 putative transmembrane domains. using the gus marker gene driven by the bcnrt1 promoter, we found bcnrt1 expression to be concentrated in primary and lateral r ...201222539185
food safety assessment of planting patterns of four vegetable-type crops grown in soil contaminated by electronic waste activities.a field experiment was conducted to assess the effect of crop and planting pattern on levels of cadmium (cd), lead (pb), and copper (cu) in crops grown in soil contaminated by electronic waste. the crops were maize (zea mays l. var. shentian-1), tomato (solanum lycopersicum l. var. zhongshu-4), cabbage (brassica oleracea l. var. jingfeng-1), and pakchoi (brassica chinensis (l.) makino. var. youdonger-hangzhou). the planting patterns were crop monoculture, crop co-planted with a legume, and crop ...201222054567
[effects of different applied nitrogen forms on pakchoi (brassica chinensis) growth and its carbon and nitrogen accumulation].a hydroponic experiment was conducted to study the effects of different applied nitrogen forms, i.e., ammonium, nitrate, glycine, glutamine, alanine, bovine serum albumin (bsa), mixture of glycine and nitrate, and mixture of bsa and nitrate, on the growth and carbon and nitrogen accumulation of pakchoi (brassica chinensis). the significant differences were observed in the b. chinensis dry mass, fresh mass, carbon and nitrogen accumulation, and soluble protein, soluble sugar, and free amino acid ...201222803472
[effects of nitrogen regulators on fertilizer nitrogen transformation in meadow cinnamon soil and on pakchoi growth].soil incubation test and pot experiment were conducted to investigate the effects of dicyandiamide (dcd) and its combination with nano-carbon on the transformation of fertilizers (urea and ammonium bicarbonate) nitrogen (n) in meadow cinnamon soil, a typical soil type in north china plain, and on the growth of pakchoi (brassica chinensis). in the first two weeks after applying urea and ammonium bicarbonate, the soil nh4+-n and no3(-)-n contents varied greatly, but little variation was observed s ...201223286007
Altered transfer of heavy metals from soil to Chinese cabbage with film mulching.The influence of film mulching on the migration of metals from soil to cabbage was investigated. Following a 50-day growth in field plots mulched or unmulched, root-zone soils and Chinese cabbage (Brassica chinensis L.) were sampled for metal analysis. Mulching slightly decreased the soil mobile (acid-extractable) Cd, but increased its transfer from root to the cabbage parts. As an essential element, Cu was readily transferred to the cabbage parts. While mulching decreased the soil mobile Zn, re ...201222036267
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