Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| genotyping of single spore isolates of a pasteuria penetrans population occurring in florida using snp-based markers. | to generate single spore lines of a population of bacterial parasite of root-knot nematode (rkn), pasteuria penetrans, isolated from florida and examine genotypic variation and virulence characteristics exist within the population. | 2017 | 27862724 |
| some aspects of the natural control of plant parasitic nematodes in soil under broad bean vicia faba l. cultivated in crop rotation and long-term monoculture. | observations on population density of plant parasitic nematodes occurring in rhizosphere of broad bean cultivated in the crop rotation and long-term monoculture were performed during 2013-2014. 13 species were observed: trichodorus primitivus, t. viruliferus, paratrichodorus pachydermus, criconema annuliferum, paratylenchus projectus, bitylenchus dubius, merlinius brevidens, pratylenchus fallax, p. flakkensis, p. neglectus, heterodera triffolii, h. goettingiana, and ditylenchus dipsaci. in monoc ... | 2015 | 27145570 |
| pasteuria penetrans for control of meloidogyne incognita on tomato and cucumber, and m. arenaria on snapdragon. | meloidogyne incognita and meloidogyne arenaria are important parasitic nematodes of vegetable and ornamental crops. microplot and greenhouse experiments were conducted to test commercial formulations of the biocontrol agent pasteuria penetrans for control of m. incognita on tomato and cucumber and m. arenaria on snapdragon. three methods of application for p. penetrans were assessed including seed, transplant, and post-plant treatments. efficacy in controlling galling and reproduction of the two ... | 2015 | 26527842 |
| interactions between nematodes and their microbial enemies in coastal sand dunes. | european foredunes are almost exclusively colonised by ammophila arenaria, and both the natural succession and the die-out of this plant have been linked to populations of plant-parasitic nematodes (ppn). the overarching aim of this study was to investigate top-down control processes of ppn in these natural ecosystems through comparative analyses of the diversity and dynamics of ppn and their microbial enemies. our specific aims were, first, to identify and quantify ppn microbial enemies in euro ... | 2012 | 22622874 |
| identification of new snp- based markers for inter and intra-species discrimination of obligate bacterial parasites (pasteuria spp.) of invertebrates. | protein encoding and 16s rrna genes of pasteuria penetrans populations from a wide range of geographic locations were examined. most inter-population single nucleotide polymorphisms (snps) were detected in the 16s rrna gene. however, in order to fully resolve all populations these were supplemented with snps from protein-encoding genes in a multi-locus snp typing approach. examination of individual 16s rrna gene sequences revealed the occurrence of 'cryptic' snps which were not present in the co ... | 2011 | 21803895 |
| a method for release and multiple strand amplification of small quantities of dna from endospores of the fastidious bacterium pasteuria penetrans. | to establish a reliable protocol to extract dna from pasteuria penetrans endospores for use as template in multiple strand amplification, thus providing sufficient material for genetic analyses. to develop a highly sensitive pcr-based diagnostic tool for p. penetrans. | 2010 | 20302597 |
| population dynamics of meloidogyne arenaria and pasteuria penetrans in a long-term crop rotation study. | the endospore-forming bacterium pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.). the primary objective of this study was to determine the effect of crop sequence on abundance of p. penetrans. the experiment was conducted from 2000 to 2008 at a field site naturally infested with both the bacterium and its host meloidogyne arenaria and included the following crop sequences: continuous peanut (arachis hypogaea) (p-p-p) and peanut rotated with either 2 years of ... | 2009 | 22736828 |
| understanding the interaction between an obligate hyperparasitic bacterium, pasteuria penetrans and its obligate plant-parasitic nematode host, meloidogyne spp. | pasteuria penetrans is an endospore-forming bacterium, which is a hyperparasite of root-knot nematodes meloidogyne spp. that are economically important pests of a wide range of crops. the life cycle of the bacterium and nematode are described with emphasis on the bacterium's potential as a biocontrol agent. two aspects that currently prohibit the commercial development of the bacterium as a biocontrol agent are the inability to culture it outside its host and its host specificity. vegetative gro ... | 2009 | 19289196 |
| genetic and immunological comparison of the cladoceran parasite pasteuria ramosa with the nematode parasite pasteuria penetrans. | pasteuria penetrans, an obligate endospore-forming parasite of meloidogyne spp. (root knot nematodes), has been identified as a promising agent for biocontrol of these destructive agricultural crop pests. pasteuria ramosa, an obligate parasite of water fleas (daphnia spp.), has been shown to modulate cladoceran populations in natural ecosystems. selected sporulation genes and an epitope associated with the spore envelope of these related species were compared. the sige and spoiiaa/spoiiab genes ... | 2008 | 17933927 |
| inter- and intra-specific cuticle variation between amphimictic and parthenogenetic species of root-knot nematode (meloidogyne spp.) as revealed by a bacterial parasite (pasteuria penetrans). | specific host-parasite interactions exist between species and strains of plant parasitic root-knot nematodes and the gram-positive bacterial hyperparasite pasteuria penetrans. this bacterium produces endospores that adhere to the cuticle of migrating juveniles, germinate and colonise the developing female within roots. endospore attachment of p. penetrans populations to second-stage juveniles of the root-knot nematode species meloidogyne incognita and meloidogyne hapla showed there were interact ... | 2008 | 18171577 |
| multiple-strand displacement and identification of single nucleotide polymorphisms as markers of genotypic variation of pasteuria penetrans biotypes infecting root-knot nematodes. | pasteuria species are endospore-forming obligate bacterial parasites of soil-inhabiting nematodes and water-inhabiting cladocerans, e.g. water fleas, and are closely related to bacillus spp. by 16s rrna gene sequence. as naturally occurring bacteria, biotypes of pasteuria penetrans are attractive candidates for the biocontrol of various meloidogyne spp. (root-knot nematodes). failure to culture these bacteria outside their hosts has prevented isolation of genomic dna in quantities sufficient for ... | 2007 | 17578522 |
| transfer and development of pasteuria penetrans. | pasteuria penetrans isolate p-20 has been attributed as the cause of soil suppressiveness to peanut root-knot nematode in florida. in this study, p. penetrans was transferred from a suppressive site to a new site and established by growing susceptible hosts to the peanut root-knot nematode during both summer and winter seasons. when two soil fumigants, 1,3-dichloropropene (1,3-d) and chloropicrin, were applied broadcast at the rate of 168 liters/ha and 263 kg/ha, respectively, the bacterium was ... | 2007 | 19259476 |
| a method for isolation of pasteuria penetrans endospores for bioassay and genomic studies. | a rapid method for collection of pasteuria penetrans endospores was developed. roots containing p. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. this method obviates laborious handpicking of pasteuria-infected females and yields endospores competent to attach to and infect nematodes. endospores are suitable for morphology studies and dna preparations. | 2006 | 19259442 |
| distribution and downward movement of pasteuria penetrans in field soil. | endospores of pasteuria penetrans were evaluated for their vertical distribution in field soil and their downward movement through soil in the laboratory. in the field trial, the number of endospores attached to second-stage juveniles (j2) of meloidogyne arenaria race 1 varied greatly in different soil depths. there were higher percentages of j2 with endospores attached in former weed fallow plots during the first 3 years of growing peanut than in former bahiagrass and rhizomal peanut plots (p < ... | 2005 | 19262855 |
| the effect of different initial densities of nematode (meloidogyne javanica) on the build-up of pasteuria penetrans population. | pasteuria penetrans will build-up faster where there is a high initial nematode density and can suppress root-knot nematode populations in the roots of tomato plants. the effect of different initial densities of nematode (meloidogyne javanica) (150, 750, 1500, 3000) and p. penetrans infected females (f1, f3) densities (f0=control and ac=absolute control without nematode or p. penetrans inoculum) on the build-up of pasteuria population was investigated over four crop cycles. two major points of i ... | 2005 | 15633246 |
| development of pasteuria penetrans in meloidogyne javanica females as affected by constantly high vs fluctuating temperature in an in-vivo system. | growth room and glasshouse experiment was conducted to investigate the effect of constant and fluctuating temperatures on the development of pasteuria penetrans a hyperparasite of root-knot nematodes. tomato plants (lycopersicon esculentum mill) were inoculated with meloidogyne javanica second-stage juveniles attached with endospores of p. penetrans and were grown in growth room at 26-29 degrees c and in glasshouse at 20-32 degrees c. the tomato plants were sampled from the growth room after 600 ... | 2005 | 15682497 |
| phylogenetic analysis of pasteuria penetrans by use of multiple genetic loci. | pasteuria penetrans is a gram-positive, endospore-forming eubacterium that apparently is a member of the bacillus-clostridium clade. it is an obligate parasite of root knot nematodes (meloidogyne spp.) and preferentially grows on the developing ovaries, inhibiting reproduction. root knot nematodes are devastating root pests of economically important crop plants and are difficult to control. consequently, p. penetrans has long been recognized as a potential biocontrol agent for root knot nematode ... | 2005 | 16077116 |
| structural analysis of divalent metals binding to the bacillus subtilis response regulator spo0f: the possibility for in vitro metalloregulation in the initiation of sporulation. | the presence of a divalent metal ion in a negatively charged aspartic acid pocket is essential for phosphorylation of response regulator proteins. here, we present metal binding studies of the bacillus subtilis response regulator spo0f using nmr and microesi-ms. nmr studies show that the divalent metals ca(2+), mg(2+) and mn(2+) primarily bind, as expected, in the asp pocket phosphorylation site. however, identical studies with cu(2+) show distinct binding effects in three specific locations: (i ... | 2005 | 16333746 |
| biological control potential of the obligate parasite pasteuria penetransagainst the root-knot nematode, meloidogyne incognita infestation in brinjal. | the efficacy of the obligate bacterial parasite, pasteuria penetrans against the rootknot nematode, meloidogyne incognita infestation was assessed in brinjal. the seedling pans with sterilized soil were inoculated with nematodes and root powder of p. penetrans were applied at different dosages viz., 0 x 10(6), 0.5 x 10(6) spores and 1 x 10(6) spores/pan. seeds of brinjal cv co2 were sown in the pans and seedlings were allowed to grow. the seedlings were transplanted to microplots containing ster ... | 2005 | 16628937 |
| integrated management of root-knot nematode, meloidogyne incognita infestation in tomato and grapevine. | an integrated approach with the obligate bacterial parasite, pasteuria penetrans and nematicides was assessed for the management of the root-knot nematode, meloidogyne incognita infestation in tomato and grapevine. seedlings of tomato cv. co3 were transplanted into pots filled with sterilized soil and inoculated with nematodes (5000 juveniles/pot). the root powder of p. penetrans at 10 mg/pot was applied alone and in combination with carbofuran at 6 mg/pot. application of p. penetrans along with ... | 2005 | 16628938 |
| persistence and suppressiveness of pasteuria penetrans to meloidogyne arenaria race. | the long-term persistence and suppressiveness of pasteuria penetrans against meloidogyne arenaria race 1 were investigated in a formerly root-knot nematode suppressive site following 9 years of continuous cultivation of three treatments and 4 years of continuous peanut. the three treatments were two m. arenaria race 1 nonhost crops, bahiagrass (paspalum notatum cv. pensacola var. tifton 9), rhizomal peanut (arachis glabrata cv. florigraze), and weed fallow. two root-knot nematode susceptible wee ... | 2004 | 19262836 |
| detection of pasteuria penetrans infection in meloidogyne arenaria race 1 in planta by polymerase chain reaction. | we report on the development of a pcr-based assay to detect pasteuria penetrans infection of meloidogyne arenaria in planta using specific primers for recently sequenced sige, spoiiab and atpf genes of p. penetrans biotype p20. amplification of these genes in crude dna extracts of ground tomato root galls using real-time kinetic pcr distinguished infected from uninfected m. arenaria race 1 by analysis of consensus thresholds for single copy genes. fluorescent in situ hybridization (fish) using t ... | 2004 | 19712314 |
| environmental quantification of pasteuria penetrans endospores using in situ antigen extraction and immunodetection with a monoclonal antibody. | abstract pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.) that has attracted significant attention as a promising biocontrol agent. the inability to culture p. penetrans has invoked the need for a quantitative detection capability to facilitate biocontrol studies. a chemical extraction method using urea, dithiothreitol and ches buffer (udc) is shown to release soluble endospore envelope antigen from endospores present in complex matrices, generating an extrac ... | 2003 | 19719647 |
| temporal formation and immunolocalization of an endospore surface epitope during pasteuria penetrans sporogenesis. | the synthesis and localization of an endospore surface epitope associated with the development of pasteuria penetrans was determined using a monoclonal antibody (mab) as a probe. nematodes, uninfected or infected with p. penetrans, were harvested at 12, 16, 24, and 38 days after inoculation (dai) and then examined to determine the developmental stage of the bacterium. vegetative growth of p. penetrans was observed only in infected nematodes harvested at 12 and 16 dai, whereas cells at different ... | 2003 | 19262762 |
| detection and characterization of pasteuria 16s rrna gene sequences from nematodes and soils. | various bacterial species in the genus pasteuria have great potential as biocontrol agents against plant-parasitic nematodes, although study of this important genus is hampered by the current inability to cultivate pasteuria species outside their host. to aid in the study of this genus, an extensive 16s rrna gene sequence phylogeny was constructed and this information was used to develop cultivation-independent methods for detection of pasteuria in soils and nematodes. thirty new clones of paste ... | 2003 | 12656160 |
| 'candidatus pasteuria usgae' sp. nov., an obligate endoparasite of the phytoparasitic nematode belonolaimus longicaudatus. | taxonomically relevant characteristics of a fastidiously gram-positive, obligately endoparasitic prokaryote (strain s-1) that uses the phytoparasitic sting nematode belonolaimus longicaudatus as its host are reviewed. 16s rdna sequence similarity (> or = 93%) confirms its congeneric ranking with other pasteuria species and strains from nematodes and cladocerans and corroborates morphological, morphometric and host range evidence suggesting a novel taxon. the 16s rdna sequence of strain s-1 has g ... | 2003 | 12656173 |
| phylogenetic analysis and confirmation of the endospore-forming nature of pasteuria penetrans based on the spo0a gene. | pasteuria penetrans is an obligate parasite of plant parasitic nematodes and has yet to be grown in vitro. we have cloned the pivotal sporulation gene, spo0a, which is the first whole gene yet to come from this organism. partial spo0a sequences were also obtained from the related bacteria, pasteuria ramosa and alicyclobacillus acidocaldarius. phylogenetic analyses using the spo0a sequence data from this and previous studies confirmed the closeness of the genera pasteuria and members of the super ... | 2003 | 12951249 |
| interactions between bacteria and plant-parasitic nematodes: now and then. | based on genome-to-genome analyses of gene sequences obtained from plant-parasitic, root-knot nematodes (meloidogyne spp.), it seems likely that certain genes have been derived from bacteria by horizontal gene transfer. strikingly, a common theme underpinning the function of these genes is their apparent direct relationship to the nematodes' parasitic lifestyle. phylogenetic analyses implicate rhizobacteria as the predominant group of 'gene donor' bacteria. root-knot nematodes and rhizobia occup ... | 2003 | 13678641 |
| pasteuria spp.: systematics and phylogeny of these bacterial parasites of phytopathogenic nematodes. | pasteuria spp. include endospore-forming bacterial pathogens of cladoceran crustaceans and plant-parasitic nematodes. propagation of these nematode pathogens requires attachment of soilborne endospores to nematode hosts, infection, growth, sporulation, and release of endospores to repeat the cycle of infection and propagation. the ability of these bacteria to suppress the levels of plant-parasitic nematodes in the field has made them particularly promising candidates for biocontrol of nematode d ... | 2003 | 19265995 |
| cuticle heterogeneity as exhibited by pasteuria spore attachment is not linked to the phylogeny of parthenogenetic root-knot nematodes (meloidogyne spp.). | the cuticle is a major barrier prohibiting the infection of nematodes against micro-organisms. the attachment of bacterial spores of the nematode hyperparasite pasteuria penetrans (pp1) to field populations of root-knot nematodes (rkn, meloidogyne spp.) from burkino faso, ecuador, greece, malawi, senegal and trinidad and tobago were assayed in standard attachment tests. the attachment of spore population pp1 to different field populations of root-knot nematode showed that the rates of attachment ... | 2001 | 11197759 |
| the interaction between the gelatin-binding domain of fibronectin and the attachment of pasteuria penetrans endospores to nematode cuticle. | pasteuria penetrans is a gram-positive endospore-producing bacterium that is a parasite of root-knot nematodes. attachment of endospores to the cuticle of the nematode is the first stage in the infection process. western blot analysis with monoclonal and polyclonal antibodies that recognize the 30 kda heparin-binding domain (hbd) and the 45 kda gelatin-binding domain (gbd) fragments of human fibronectin (fn) revealed a series of polypeptides of approximately 40, 45 and 55 kda present in crude cu ... | 2001 | 11578090 |
| apomictic, polyphagous root-knot nematodes: exceptionally successful and damaging biotrophic root pathogens. | most apomictic root-knot nematodes (rkn; meloidogyne spp.) have host ranges that encompass the majority of flowering plants, and m. incognita is possibly the world's most damaging crop pathogen. the ancestors, age, and origins of the polyphagous rkn are obscure, but there is increasing evidence that m. incognita, m. javanica, and m. arenaria are closely related, heterogeneous species with a recent, hybrid (reticulate) origin. if so, they must owe much of their current worldwide distributions to ... | 2001 | 11701859 |
| extraction and purification of pasteuria spp. endospores. | pasteuria penetrans is an endospore-forming bacterial parasite of root-knot nematodes that has potential as a biological control agent. biochemical investigations of p. penetrans are limited because of difficulty in obtaining large quantities of endospores free of plant debris and contaminating microorganisms. our objective was to develop a technique for extraction and purification of p. penetrans endospores from root-knot nematodes. tomato roots infected with meloidogyne arenaria that was paras ... | 2000 | 19270952 |
| effects of fluctuating temperatures and different host plants on development of pasteuria penetrans in meloidogyne javanica. | greenhouse and growth room experiments were conducted to investigate the effect of host plant in relation to different nematode inoculum levels, and temperature fluctuations on the development of pasteuria penetrans. host plant affected the development of p. penetrans indirectly through its effect on nematode development. endospores collected from meloidogyne javanica females reared on different hosts did not show any differences in subsequent attachment and infectivity. the numbers of endospore ... | 1999 | 19270902 |
| phylogenetic analysis of pasteuria penetrans by 16s rrna gene cloning and sequencing. | pasteuria penetrans is an endospore-forming bacterial parasite of meloidogyne spp. this organism is among the most promising agents for the biological control of root-knot nematodes. in order to establish the phylogenetic position of this species relative to other endospore-forming bacteria, the 16s ribosomal genes from two isolates of p. penetrans, p-20, which preferentially infects m. arenaria race 1, and p-100, which preferentially infects m. incognita and m. javanica, were pcr-amplified from ... | 1999 | 19270903 |
| attachment tests of pasteuria penetrans to the cuticle of plant and animal parasitic nematodes, free living nematodes and srf mutants of caenorhabditis elegans. | populations of pasteuria penetrans isolated from root-knot nematodes (meloidogyne spp.) and cyst nematodes (heterodera spp.) were tested for their ability to adhere to a limited selection of sheathed and ex-sheathed animal parasitic nematodes, free living nematodes, including caenorhabditis elegans wild type and several srf mutants, and plant parasitic nematodes. the attachment of spores of pasteuria was restricted and no spores were observed adhering to any of the animal parasitic nematodes eit ... | 1999 | 10431371 |
| observations on a pasteuria isolate parasitic on hoplolaimus galeatus in peru. | a pasteuria isolate associated with a population of the lance nematode hoplolaimus galeatus was discovered in peru. the infective propagules adhered to adult stages and juveniles and were found filling the bodies of males and females. the endospore and central core diameters measured 4.5 +/- 0.4 pm and 1.9 +/- 0.2 mum, respectively, which differed from those reported for other pasteuria isolates found iu north america on the same host. examinations of endospore ultrastructure with scanning elect ... | 1998 | 19274212 |
| review of pasteuria penetrans: biology, ecology, and biological control potential. | pasteuria penetrans is a mycelial, endospore-forming, bacterial parasite that has shown great potential as a biological control agent of root-knot nematodes. considerable progress has been made during the last 10 years in understanding its biology and importance as an agent capable of effectively suppressing root-knot nematodes in field soil. the objective of this review is to summarize the current knowledge of the biology, ecology, and biological control potential of p. penetrans and other past ... | 1998 | 19274225 |
| minimal growth temperature of pasteuria penetrans. | pasteuria penetrans is an obligate, mycelial, and endospore-forming bacterial parasite of meloidogyne spp. with promise for the management of root-knot nematodes. our objective was to use regression analysis of developmental time (days) to various temperatures to determine the minimal temperature for growth and development of p. penetrans in meloidogyne spp. the data set for regression originated from a previously published report. the data fit well to hyperbolic equations. for various developme ... | 1997 | 19274263 |
| suppression mechanisms of meloidogyne arenaria race 1 by pasteuria penetrans. | the biological control of meloidogyne arenaria on peanut (arachis hypogaea) by pasteuria penetrans was evaluated using a six x six factorial experiment in field microplots over 2 years. the main factors were six inoculum levels of second-stage juveniles (j2) of m. arenaria race 1 (0, 40, 200, 1,000, 5,000, and 25,000 j2/microplot, except that the highest level was 20,000 j2/microplot in 1995) and six infestation levels of p. penetrans as percentages of j2 with endospores attached (0, 20, 40, 60, ... | 1997 | 19274127 |
| temperature-dependent development of pasteuria penetrans in meloidogyne arenaria. | pasteuria penetrans is a promising biological control agent of plant-parasitic nematodes. this study was conducted to determine effects of temperature on the bacterium's development in meloidogyne arenaria. developmental stages of p. penetrans were viewed with a compound microscope and verified with scanning electron microscopy within each nematode at 100 accumulated degree-day intervals by tracking accumulated degree-days at three temperatures (21, 28, and 35 degrees c). five predominant develo ... | 1997 | 19274154 |
| antibodies from chicken eggs as probes for antigens from pasteuria penetrans endospores. | the bacteria pasteuria spp. have been identified as among the most promising of several microbial organisms currently under investigation as biological control agents of plant-parasitic nematodes. as part of our goal to develop methods to discriminate isolates of pasteuria penetrans with different host preferences, we investigated the potential of developing antibody probes to identify endospores of different isolates of p. penetrans. polyclonal igy antibodies were raised in chickens against end ... | 1997 | 19274158 |
| estimating incidence of attachment of pasteuria penetrans endospores to meloidogyne spp. with tally thresholds. | pasteuria penetrans has .been identified as an important biological control agent of root-knot nematodes. in this study the use of tally thresholds was evaluated for estimating p. penetrans endospore attachment to second-stage juveniles (j2) of meloidogyne spp. a tally threshold (t) is defined as the maximum number of individuals in a sample unit that may be treated as absent based on binomial sampling. three different data sets that originated from centrifugal bioassay, incubation bioassay, and ... | 1997 | 19274161 |
| temperature effects on the attachment of pasteuria penetrans endospores to meloidogyne arenaria race 1. | pasteuria penetrans is a gram positive bacterium that prevents meloidogyne spp. from reproducing and diminishes their ability to penetrate roots. the attachment of the endospores to the cuticle of the nematodes is the first step in the life cycle of the bacterium and is essential for its reproduction. as a preliminary study to a field solarization test, the effects of temperature on the attachment of p. penetrans on meloidogyne arenaria race 1 were investigated. preexposing second-stage juvenile ... | 1997 | 19274192 |
| effects of monoclonal antibodies, cationized ferritin, and other organic molecules on adhesion of pasteuria penetrans endospores to meloidogyne incognita. | the incidence of adhesion of pasteuria penetrans endospores to meloidogyne incognita second-stage juveniles (j2) was studied after pretreatment of the latter with monoclonal antibodies (mab), cationized ferritin, and other organic molecules in replicated trials. monoclonal antibodies developed to a cuticular epitope of m. incognita second-stage juveniles gave significant reductions in attachment of p. penetrans endospores to treated nematodes. mab bound to the entire length of j2 except for the ... | 1997 | 19274193 |
| ultrastructure, morphology, and sporogenesis of pasteuria penetrans. | abstract pasteuria penetrans is a bacterial parasite of root-knot nematodes that shows great potential as a biocontrol agent. scanning and transmission electron microscopy were used to study the ultrastructure, morphology, and sporogenesis of four isolates of p. penetrans. the effects of different meloidogyne spp. and tobacco cultivars on sporangium size and morphology of endospores attached to the cuticle of second-stage juveniles (j2) of root-knot nematodes also were investigated. the p. penet ... | 1997 | 18945170 |
| [the preparing of bacillus penetrans preparation and their controlling to root-knot nematodes]. | in order to make use of b. penetrans the preparation of these bacteria was prepared successfully. each gram of the specimen contains more than 3.1 x 10(8) spores according to determination by bloodcount plate. the method of its production are: first, infect the larvae of root-knot nematodes with the spores of b. penetrans. second, inoculate infectable plants with the infected larvae. third, beat the root-systems of inoculated plants after adding some water. the flowed liquid from a bronze sieve ... | 1997 | 11189383 |
| diversity and partial characterization of putative virulence determinants in pasteuria penetrans, the hyperparasitic bacterium of root-knot nematodes (meloidogyne spp.). | antigens recognized by monoclonal antibodies (mabs) raised to the surface of the obligate nematode hyperparasite pasteuria penetrans were characterized. using the attachment of spores of the bacterium to host nematodes to determine the biological variability present on the spore surface greatly underestimated the amount of surface heterogeneity present compared with estimates from immunological techniques. this heterogeneity differed not only between different individual spores from the same pop ... | 1997 | 9281826 |
| modulation of spore adhesion of the hyperparasitic bacterium pasteuria penetrans to nematode cuticle. | monoclonal antibodies (mab) raised to the cuticule surface of second-stage juveniles (j2) of the nematode heterodera cajani were partially characterized by immunofluorescence and western blot analysis. five antigens with relative molecular weights (m(r)) 55, 80, 110, 180 and 210 kda were identified with six mab. pasteuria spores, originating from the same population of h. cajani to which the antibodies were raised, were tested for their ability to attach to j2, which had been pretreated with eac ... | 1997 | 9449856 |
| suppression of meloidogyne arenaria race 1 by soil application of endospores of pasteuria penetrans. | the potential of pasteuria penetrans for suppressing meloidogyne arenaria race 1 on peanut (arachis hypogaea) was tested over a 2-year period in a field microplot experiment. endospores of p. penetrans were mass-produced on m. arenaria race 1 infecting tomato plants. endospores were inoculated in the first year only at rates of 0, 1,000, 3,000, 10,000, and 100,000 endospores/g of soil, respectively, into the top 20 cm of microplots that were previously infested with m. arenaria race 1. one peanu ... | 1996 | 19277131 |
| attachment of pasteuria penetrans endospores to the surface of meloidogyne javanica second-stage juveniles. | pasteuria penetrans spore adhesion to meloidogyne javanica second-stage juveniles (j2) was examined following several different pretreatments of the latter. the detergents sodium dodecyl sulfate and triton x-100, the carbohydrates fucose and alpha-methyl-d-mannoside, and the lectins concanavalin a and wheat germ agglutinin reduced spore attachment. spores exposed to m. javanica surface coat (sc) extract exhibited decreased adherence to the j2 surface. second-stage juveniles that had been treated ... | 1996 | 19277150 |
| suppression of meloidogyne incognita and m. javanica by pasteuria penetrans in field soil. | the role of pasteuria penetrans in suppressing numbers of root-knot nematodes was investigated in a 7-year monocuhure of tobacco in a field naturally infested with a mixed population of meloidogyne incognita race 1 and m. javanica. the suppressiveness of the soil was tested using four treatments: autoclaving (ac), microwaving (mw), air drying (dr), and untreated. the treated soil bioassays consisted of tobacco cv. northrup king 326 (resistant to m. incognita but susceptible to m. javanica) and c ... | 1996 | 19277344 |
| quantification of endospore concentrations of pasteuria penetrans in tomato root material. | six methods for quantification of the endospore concentrations of pasteuria penetrans from tomato roots are described. mortar disruption and machine disruption methods gave the highest estimations (endospores per gram of root material) of 83.7 and 79.0 million, respectively. these methods were significantly superior to incubation bioassay (47.7 million), enzymatic disruption (32.1 million), and enzymatic disruption + flotation (25.8 million) methods. a centrifugation bioassay method gave the low ... | 1996 | 19277345 |
| the surface coat of plant-parasitic nematodes: chemical composition, origin, and biological role-a review. | chemical composition, origin, and biological role of the surface coat (sc) of plant-parasitic nematodes are described and compared with those of animal-parasitic and free-living nematodes. the sc of the plant-parasitic nematodes is 5-30 nm thick and is characterized by a net negative charge. it consists, at least in part, of glycoproteins and proteins with various molecular weights, depending upon the nematode species. the lability of its components and the binding of human red blood cells to th ... | 1995 | 19277272 |
| atomic force microscopy of plant-parasitic nematodes. | a simple method for atomic force microscopy (afm) of nematode cuticle was developed to visualize the external topography of helicotylenchus lobus, meloidogyne javanica, m. incognita, and xiphinema diversicaudatum. endospores of two isolates of the nematode parasite, pasteuria penetrans, adhering to m. incognita and x. diversicaudatum were also visualized and measured by this technique. scanning procedures were applied to specimens killed and dehydrated in air or dehydrated and stored in glycerol ... | 1995 | 19277280 |
| occurrence of pasteuria spp. in florida. | two years of data collected from the florida nematode assay laboratory of the florida cooperative extension service and 4 years of data from the florida department of agriculture and consumer services, division of plant industry, were compiled to find out the distribution of pasteuria spp. on nematodes in florida soils. information recorded came from 335 samples and included nematode genera with pasteuria endospores attached, host plants associated with the samples, and the origins of the sample ... | 1994 | 19279936 |
| response of meloidogyne spp. to pasteuria penetrans, fungi, and cultural practices in tobacco. | the response of a mixed population of meloidogyne incognita and m. javanica to three cultural practices, tobacco cultivars (two cultivars, differing in resistance to m. incognita), cover treatments (three treatments), and inorganic nitrogen fertilizer (two treatments), pasteuria penetrans, and soil-borne fungi was investigated in a tobacco field in 1991. on all sampling dates, higher densities of root-knot nematodes were observed on tobacco cv. coker 371 gold than on k-326. initially, forage sor ... | 1994 | 19279937 |
| a centrifugation method for attaching endospores of pasteuria spp. to nematodes. | attachment of relatively low numbers of endospores from two isolates of pasteuria spp. to several species of nematodes was consistently achieved in 2-5 minutes with a centrifugation technique. the rate of attachment of pasteuria penetrans at 10 endospores/0.1 ml/tube to second-stage juveniles (j2) of meloidogyne javanica, m. incognita race 1, m. incognita race 3, and m. arenaria races 1 and 2 in two tests averaged 4.4, 5.2, 0.1, 0.3, and 0 endospores per j2, respectively. the rate of attachment ... | 1993 | 19279840 |
| effect of temperature on attachment, development, and interactions of pasteuria penetrans on meloidogyne incognita. | the effect of temperature (10, 20, 25, 30, and 35 c) on attachment and development of pasteuria penetrans on meloidogyne arenaria race 1 was elevated in growth chambers. the greatest attachment rate of endospores of p. penetrans occurred on second-stage juveniles at 30 c. the bacterium developed more quickly within its host at 30 and 35 c than at 25 c or below. the development of the bacterium within the nematode female was divided into nine recognizable life stages, which ranged from early vege ... | 1992 | 19283029 |
| parasitism of helicotylenchus lobus by pasteuria penetrans in naturally infested soil. | the population density of helicotylenchus lobus and the percentage of the population with spores of pasteuria penetrans were determined for 10 monthly intervals in naturally infested turf grass soil at riverside, california. the percentage of nematodes with attached spores ranged from 40% to 67%. no relationship was found between nematode density and the percentage of nematodes with spores. the mean and maximum numbers of spores adhering per nematode with at least one spore ranged from 2 to 8 an ... | 1992 | 19283198 |
| population development of pasteuria penetrans on meloidogyne arenaria. | a microplot study on the influence of cropping sequences with peanut in summer and bare fallowed or cover crops of rye or vetch in winter on the population development of pasteuria penetrans was initiated in the spring of 1987. the number of spores of p. penetrans attached per second-stage juvenile of meloidogyne arenaria race 1 increased from 0.11 in the fall of 1987 to 7.6, 8.6, and 3.6 in the fall of 1989 in the rye, vetch, and fallowed plots, respectively. higher (p </= 0.05) levels of p. pe ... | 1991 | 19283094 |
| specific gravity of spores of pasteuria penetrans and extraction of spore-filled nematodes from soil. | the specific gravity of spores of pasteuria penetrans collected from meloidogyne arenaria was found to be around 1.28. increasing the sucrose concentration used for the extraction of pratylenchus scribneri from a specific gravity of 1.14 to 1.26 led to the recovery of higher numbers of specimens filled with spores of pasteuria sp. (p </= 0.05). the numbers of spore-filled specimens of hoplolaimus galeatus recovered from field soil were not affected by the concentration of the sucrose solutions. ... | 1991 | 19283193 |
| characterisation of potential adhesins of the bacterium pasteuria penetrans, and of putative receptors on the cuticle of meloidogyne incognita, a nematode host. | pasteuria penetrans spores were fragmented by glass bead vortexing, producing exosporial membranes and spore fragments, which consisted of fibre bundles. both exosporia and spore fragments are capable of host-specific attachment to the cuticle of meloidogyne incognita, a root-knot nematode host. putative m. incognita receptors appear to be soluble in beta-mercaptoethanol (bme) but not sds, and are also sensitive to tryptic digestion and deglycosylation by endoglycosidase f. polyclonal antibodies ... | 1991 | 1808209 |
| pasteuria nishizawae sp. nov., a mycelial and endospore-forming bacterium parasitic on cyst nematodes of genera heterodera and globodera. | this study describes pasteuria nishizawae sp. nov., a fourth species of the genus pasteuria. this mycelial and endospore-forming bacterium parasitizes the adult females of cyst-forming nematodes in the genera heterodera and globodera. the distinct ultrastructural features and unique host range found for this bacterium separate it from two closely related species, pasteuria penetrans, which parasitizes several species of root-knot nematodes of the genus meloidogyne, and pasteuria thornei, which a ... | 1991 | 1947427 |
| host range and ecology of isolates of pasteuria spp. from the southeastern united states. | isolates of pasteuria penetrans were evaluated for ecological characteristics that are important in determining their potential as biological control agents. isolate p-20 survived without loss of its ability to attach to its host nematode in dry, moist, and wet soil and in soil wetted and dried repeatedly for 6 weeks. some spores moved 6.4 cm (the maximum distance tested) downward in soil within 3 days with percolating water. the isolates varied greatly in their attachment to different nematode ... | 1990 | 19287753 |
| isolates of the pasteuria penetrans group from phytoparasitic nematodes in bermudagrass turf. | a survey was conducted between 1985 and 1989 of isolates of the pasteuria penetrans group on phytoparasitic nematodes in bermudagrass (cynodon spp.) turf in southern florida. six different isolates of the p. penetrans group were observed from five different species of phytoparasitic nematode hosts. five of the bacterial isolates were different (p </= 0.01) in sporangium diameter, endospore width, and ratio of sporangium diameter to endospore width. all locations surveyed had one or more isolates ... | 1990 | 19287792 |
| parasitism of the citrus nematode, tylenchulus semipenetrans, by pasteuria penetrans in iraq. | 1989 | 19287633 | |
| pasteuria thornei sp. nov. and pasteuria penetrans sensu stricto emend., mycelial and endospore-forming bacteria parasitic, respectively, on plant-parasitic nematodes of the genera pratylenchus and meloidogyne. | descriptions are presented of two members of the pasteuria penetrans group of mycelial and endospore-forming bacteria, parasitic on plant-parasitic nematodes. in one case, the epithet p. penetrans sensu stricto emend. has now been limited to members of this group with cup-shaped sporangia and ellipsoidal endospores, parasitic primarily on the root-knot nematode meloidogyne incognita. the second organism, with rhomboidal sporangia and nearly spherical endospores, which is parasitic primarily on t ... | 1988 | 3382544 |
| biological control of meloidogyne incognita by paecilomyces lilacinus and pasteuria penetrans. | the root-knot nematode meloidogyne incognita was controlled more effectively and yields of host plants were greater when paecilomyces lilacinus and pasteuria penetrans were applied together in field microplots than when either was applied alone. yields of winter vetch from microplots inoculated with the nematode and with both organisms were not statistically different from yields from uninoculated control plots. | 1987 | 19290133 |
| root penetration by meloidogyne incognita juveniles infected with bacillus penetrans. | bacillus penetrans inhibited penetration by meloidogyne incognita second-stage juveniles (j2) into tomato roots in the laboratory and greenhouse. spores from this florida population of b. penetrans attached to j2 of m. javanica, m. incognita, and m. arenaria. a greater proportion of j2 of m. javanica were infected than were j2 of either m. incognita or m. arenaria, and a greater number of spores attached to m. incognita than to m. arenaria. | 1985 | 19294069 |
| biocontrol: bacillus penetrans and related parasites of nematodes. | bacillus penetrans mankau, 1975, previously described as duboscqia penetrans thorne 1940, is a candidate agent for biocontrol of nematodes. this review considers the life stages of this bacterium: vegetative growth phase, colony fragmentation, sporogenesis, soil phase, spore attachment, and penetration into larvae of root-knot nematodes. the morphology of the microthallus colonies and the unusual external features of the spore are discussed. taxonomic affinities with the actinomycetes, particula ... | 1980 | 19300701 |
| infectivity of bacillus penetrans in plant-parasitic nematodes. | larvae of meloidogyne spp. were readily infected with the endoparasite bacillus penetrans by exposure to an aqueous suspension of spores from infected root-knot nematode females, or by passage of larvae through a shallow layer (50 cm(3)) of spore-infested soil. infection severely reduced motility of second-stage larvae through soil. bacillus penetrans exhibited a distinct host specificity in that only 5 of 16 nematode species tested became infected with the population used. meloidogyne javanica, ... | 1977 | 19305569 |
| sem observations on nematode cuticle penetration by bacillus penetrans. | 1976 | 19308218 |