Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| convenient enzymatic resolution of (r,s)-2-methylbutyric acid catalyzed by immobilized lipases. | the application of several immobilized lipases has been explored in the enantioselective esterification of (r,s)-2-methylbutyric acid, an insect pheromone precursor. with the use of candida antarctica b, using hexane as solvent, (r)-pentyl 2-methylbutyrate was prepared in 2 h with c 40%, eep 90%, and e = 35, while thermomyces lanuginosus leads to c 18%, eep 91%, and e = 26. the (s)-enantiomer was obtained by the use of candida rugosa or rhizopus oryzae (2-h reaction, c 34% and 35%, eep 75 and 49 ... | 2018 | 29083057 |
| succinic acid production from fruit and vegetable wastes hydrolyzed by on-site enzyme mixtures through solid state fermentation. | in this study, a novel biorefinery concept of succinic acid (sa) production from fruit and vegetable wastes (fvws) hydrolyzed by crude enzyme mixtures through solid state fermentation was designed. enzyme complex solid mashes from various types of fvws were on-site produced through solid-state fermentation by aspergillus niger and rhizopus oryzae. this solid was then added to fvw suspensions and undergo hydrolysis reaction to generate fermentable sugars and other essential nutrients for bacteria ... | 2018 | 28941663 |
| production of fumaric acid by immobilized rhizopus arrhizus rh 7-13-9# on loofah fiber in a stirred-tank reactor. | fumaric acid is an important building-block chemical. the production of fumaric acid by fermentation is possible. loofah fiber is a natural, biodegradable, renewable polymer material with highly sophisticated and pore structure. this work investigated a new immobilization method using loofah fiber as carrier to produce fumaric acid in a stirred-tank reactor. compared with other carriers, loofah fiber was proven to be efficiently and successfully used in the reactor. after the optimization proces ... | 2017 | 28847082 |
| mucorales spores induce a proinflammatory cytokine response in human mononuclear phagocytes and harbor no rodlet hydrophobins. | mucormycoses are life-threatening infections in immunocompromised patients. this study characterizes the response of human mononuclear cells to different mucorales and ascomycota. pbmc, monocytes, and monocyte derived dendritic cells (modcs) from healthy donors were stimulated with resting and germinated stages of mucorales and ascomycota. cytokine response and expression of activation markers were studied. both inactivated germ tubes and resting spores of rhizopus arrhizus and other human patho ... | 2017 | 28783439 |
| preparation of hydrolytic liquid from dried distiller's grains with solubles and fumaric acid fermentation by rhizopus arrhizus rh 7-13. | fumaric acid production from lignocellulosic materials is an alternative chemicals production system. this work investigated the suitable conditions for hydrolysis of dried distiller's grains with solubles (ddgs). the hydrolytic liquid was subsequently used for the production of fumaric acid. after optimizing the hydrolysis conditions, the most suitable concentration of h2so4 (2%), hydrolysis temperature (120 °c), hydrolysis time (100min) and solid/liquid ratio (1:10) were obtained. the yield of ... | 2017 | 28662421 |
| prophylactic treatment with vt-1161 protects immunosuppressed mice from rhizopus arrhizus var. arrhizus infection. | we compared prophylactic or continuous therapy with the investigational drug vt-1161 to that with posaconazole in treating murine mucormycosis due to rhizopus arrhizus var. arrhizus in the prophylaxis studies, only vt-1161 resulted in improved survival and lowered tissue fungal burden of immunosuppressed infected mice. in the continuous therapy, vt-1161 outperformed posaconazole in prolonging mouse survival time despite its comparable effect in lowering tissue fungal burden. these results suppor ... | 2017 | 28652241 |
| rhizopus arrhizus invasive infection due to self-inflicted scratch injuries in a diabetic patient with non-ketotic acidosis. | mucormycosis is a rare infection caused by members included in the subphylum mucoromycotina. characterized by the histopathological hallmark of angioinvasion, these infections affect most often patients with certain underlying conditions carrying immunosuppression (haematological neoplasias, diabetic ketoacidosis and other forms of acidosis, and iron overload) or immunocompetent patients with traumatic mucocutaneous barriers breakdown and direct inoculation of the mould. a case is presented in w ... | 2017 | 28646276 |
| co-fermentation of a mixture of glucose and xylose to fumaric acid by rhizopus arrhizus rh 7-13-9. | lignocellulose is the most abundant biomass, composed of cellulose, hemicellulose and lignin. it can be converted into glucose and xylose, which could be utilized as carbon source to produce fumaric acid. but glucose and xylose were commonly used separately to produce fumaric acid, while the co-fermentation of glucose and xylose process was not studied so far. in this work, the co-fermentation process was researched through a new strain r. arrhizus rh 7-13-9# isolated from high concentration xyl ... | 2017 | 28258993 |
| cationic surfactant-modified biosorption of anionic dyes by dried rhizopus arrhizus. | the biosorption of an anionic dye remazol black b onto a filamentous, dried fungus, rhizopus arrhizus, was studied in the presence of the cationic surfactant cetyl trimethyl ammonium bromide (ctab), which was used as a ph shifter. without surfactant, the maximum dye uptake capacity was achieved at ph 2.0 and was 434.0 mg dye g-1 dried fungal biomass. again, without surfactant, the highest dye uptake capacity, 27.8 mg dye g-1 dried biomass, occurred at ph 8.0 calculated from the langmuir adsorpti ... | 2017 | 27960635 |
| disseminated infection with multiple cold abscesses caused by rhizopus arrhizusin an immunocompetent girl. | although infections with fungi, such as rhizopus species,usually occur in diabetics or immunocompromised patients, they can also rarely affect immunocompetent individuals. we present a case of disseminated infection with multiple cold abscesses in a 13-year, otherwise healthy girl caused by rhizopus arrhizus. | 2017 | 29056129 |
| co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases. | bioethanol production from sustainable sources of biomass that limit effect on food production are needed and in a biorefinery approach co-products are desirable, obtained from both the plant material and from the microbial biomass. fungal biotransformation of steroids was among the first industrial biotransformations allowing corticosteroid production. in this work, the potential of yeast to produce intermediates needed in corticosteroid production is demonstrated at laboratory scale following ... | 2017 | 29021826 |
| salinity induced effects on the growth rates and mycelia composition of basidiomycete and zygomycete fungi. | soil salinization, as the combination of primary and secondary events, can adversely affect organisms inhabiting this compartment. in the present study, the effects of increased salinity were assessed in four species of terrestrial fungi: lentinus sajor caju, phanerochaete chrysosporium, rhizopus oryzae and trametes versicolor. the mycelial growth and biochemical composition of the four fungi were determined under three exposure scenarios: 1) exposure to serial dilutions of natural seawater (sw) ... | 2017 | 28964607 |
| fast technique for the identification of gilbertella persicaria via optical microscopy. | gilbertella persicaria is an important phytopathogen that is confused with mucor spp. and rhizopus spp. the main distinguishing characteristic of g. persicaria is the presence of appendages in sporangiospores, and their observation by conventional staining techniques generally fails. a technique is described using light microscopy for fast and reliable diagnosis. | 2017 | 28899635 |
| evaluation of pcr-reverse line blot hybridization assay for simultaneous identification of medically important saprophytic fungi. | in immunocompromised patients suffering from invasive fungal infections, rapid identification of fungal species is important since the appropriate treatment is usually related to the responsible species. we describe here, an assay based on combination of pcr and reverse line blot hybridization (pcr/rlb) for differentiation causative agent of fungal infections. | 2017 | 29100947 |
| intrinsic short-tailed azole resistance in mucormycetes is due to an evolutionary conserved aminoacid substitution of the lanosterol 14α-demethylase. | mucormycoses are emerging and potentially lethal infections. an increase of breakthrough infections has been found in cohorts receiving short-tailed azoles prophylaxis (e.g. voriconazole (vcz)). although vcz is ineffective in vitro and in vivo, long-tailed triazoles such as posaconazole remain active against mucormycetes. our goal was to validate the molecular mechanism of resistance to short-tailed triazoles in mucorales. the paralogous cytochrome p450 genes (cyp51 f1 and cyp51 f5) of rhizopus ... | 2017 | 29162893 |
| antimicrobial activity of water and acetone extracts of some eucomis taxa. | to evaluate the antibacterial and antifungal properties of acetone and water extracts of selected eucomis taxa. | 2017 | 29080618 |
| occurrence and species distribution of pathogenic mucorales in unselected soil samples from france. | mucormycosis is a life-threatening invasive fungal disease that affects a variety of patient groups. although mucorales are mostly opportunistic pathogens originating from soil or decaying vegetation, there are currently few data on prevalence of this group of fungi in the environment. the aim of the present study was to assess the prevalence and diversity of species of mucorales from soil samples collected in france. two grams of soil were homogenized in sterile saline and plated on sabouraud d ... | 2017 | 28992337 |
| identification by pcr-dgge, sds-page, and maldi-tof ms of lab and fungi in laru, a fermented starter. | laru samples obtained from the upper part of borneo island were analyzed for their lactic acid bacterial (lab) and fungal diversity using both a culture-independent method (pcr-dgge) and culture-dependent methods (sds-page and maldi-tof ms). pediococcus pentosaceus, lactobacillus brevis, saccharomycopsis fibuligera, hyphopichia burtonii, and kodamaea ohmeri were detected by all three methods. in addition, weissella cibaria, weissella paramesenteroides, leuconostoc citreum, leuconostoc mesenteroi ... | 2017 | 29081085 |
| production of cellulosic organic acids via synthetic fungal consortia. | consolidated bioprocessing (cbp) is a potential breakthrough technology for reducing costs of biochemical production from lignocellulosic biomass. production of cellulase enzymes, saccharification of lignocellulose, and conversion of the resulting sugars into a chemical of interest occur simultaneously within a single bioreactor. in this study, synthetic fungal consortia composed of the cellulolytic fungus trichoderma reesei and the production specialist rhizopus delemar demonstrated conversion ... | 2017 | 29205274 |
| metalaxyl degradation by mucorales strains gongronella sp. and rhizopus oryzae. | in this study, the degradation of metalaxyl was investigated in the presence of two mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the internal transcribed spacer (its) gene region, phenotypic characterisation by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) spectral ... | 2017 | 29240696 |
| primary cutaneous mucormycosis due to rhizopus arrhizus in an 8-year-old girl. | 2017 | 29217228 | |
| fungal-specific cyp51 inhibitor vt-1598 demonstrates in vitro activity against candida and cryptococcus species, endemic fungi, including coccidioides species, aspergillus species and rhizopus arrhizus. | invasive fungal infections, including those caused by yeasts, moulds and endemic organisms, can be significant causes of morbidity and mortality in immunocompromised hosts, those with multiple comorbidities and occasionally immunocompetent hosts. current antifungal agents are often limited by drug toxicities, drug interactions or the development of resistance. vt-1598 is a novel tetrazole that has greater specificity for fungal cyp51 than currently available triazoles and thus the potential for ... | 2017 | 29190379 |
| cutaneous granuloma caused by rhizopus oryzae with a novel mutation in the cybb gene in a monozygotic male twin. | 2017 | 29178191 | |
| mucormycosis is a rare life-threatening opportunistic infection caused by fungi of the order mucorales. we report a case of rhinocerebral mucormycosis caused by rhizopus arrhizus var. tonkinensis, the first formally reported case in the literature. early diagnosis, reversal of predisposing factors, surgical debridement and prompt administration of antifungal therapy are critical for good outcome. | 2017 | 29122529 | |
| cutaneous mucormycosis in advanced hiv disease. | angionvasive mucormycosis is an emerging fungal disease known to affect mainly diabetics or subjects with profound neutropenia. infection usually occurs through the inhalation route, but cutaneous inoculation may occur after trauma or burns. however, mucormycosis remains unusual in hiv infection. we report a fatal case of cutaneous mucormycosis due to rhizopus arrhizus involving the scalp following herpes zoster infection. the patient was a 42-year-old man with advanced aids failing on salvage a ... | 2017 | 27473891 |
| antimicrobial activity, toxicity and anti-inflammatory potential of methanolic extracts of four ethnomedicinal plant species from punjab, pakistan. | the plant species aristolochia indica (ai), melilotus indicus (mi), tribulus terrestris (tt) and cuscuta pedicellata (cp) are widely used in folk medicine in the villages around chowk azam, south punjab, pakistan. the aim of this study was to evaluate the antioxidant activity, phytochemical composition, and the antibacterial, antifungal, cytotoxic and anti-inflammatory potential of the four medicinal plants listed above. for cp stem, this study represents (to the best of our knowledge) the first ... | 2017 | 28595608 |
| evaluation of different pcr primers for denaturing gradient gel electrophoresis (dgge) analysis of fungal community structure in traditional fermentation starters used for hong qu glutinous rice wine. | denaturing gradient gel electrophoresis (dgge) has become a widely used tool to examine microbial community structure. however, when dgge is applied to evaluate the fungal community of traditional fermentation starters, the choice of hypervariable ribosomal rna gene regions is still controversial. in the current study, several previously published fungal pcr primer sets were compared and evaluated using pcr-dgge, with the purpose of screening a suitable primer set to study the fungal community o ... | 2017 | 28595086 |
| disease resistance to multiple fungal and oomycete pathogens evaluated using a recombinant inbred line population in pepper. | incorporating disease resistance into cultivars is a primary focus of modern breeding programs. resistance to pathogens is often introgressed from landrace or wild individuals with poor fruit quality into commercial-quality cultivars. sites of multiple disease resistance (mdr) are regions or "hotspots" of the genome with closely linked genes for resistance to different pathogens that could enable rapid incorporation of resistance. an f2-derived f6 recombinant inbred line population from a cross ... | 2017 | 28762287 |
| green synthesis of conjugated linoleic acids from plant oils using a novel synergistic catalytic system. | a novel and efficient method has been developed for converting plant oil into a specific conjugated linoleic acid (cla) using a synergistic biocatalytic system based on immobilized propionibacterium acnes isomerase (pai) and rhizopus oryzae lipase (rol). pai exhibited the greatest catalytic activity when immobilized on d301r anion-exchange resin under optimal conditions (pai dosage of 12 410 u of pai/g of d301r, glutaraldehyde concentration of 0.4%, and reaction conditions of ph 7.0, 25 °c, and ... | 2017 | 28470063 |
| enzymatic hydrolysis of polyester thin films at the nanoscale: effects of polyester structure and enzyme active-site accessibility. | biodegradable polyesters have a large potential to replace persistent polymers in numerous applications and to thereby reduce the accumulation of plastics in the environment. ester hydrolysis by extracellular carboxylesterases is considered the rate-limiting step in polyester biodegradation. in this work, we systematically investigated the effects of polyester and carboxylesterase structure on the hydrolysis of nanometer-thin polyester films using a quartz-crystal microbalance with dissipation m ... | 2017 | 28538100 |
| documentation of ethnomedicinal information and antimicrobial validation of thespesia populnea used by yanadi tribe of ganugapenta village, chittoor district, andhra pradesh, india. | this study aimed to document the traditional knowledge of medicinal plants and antimicrobial validation of thespesia populnea used by yanadi tribe of chittoor district, andhra pradesh, india. | 2017 | 28512597 |
| one-pot conjugated linoleic acid production from castor oil by rhizopus oryzae lipase and resting cells of lactobacillus plantarum. | conjugated linoleic acid (cla) has attracted as novel type of fatty acids having unusual health-promoting properties such as anticarcinogenic and antiobesitic effects. the present work employed castor oil as substrate for one-pot production of cla using washed cells of lactobacillus plantarum (l. plantarum) and lipases as catalysts. among the screened lipases, the lipase rhizopus oryzae (rol) greatly assisted resting cells to produce cla. mass spectral analysis of the product showed that two maj ... | 2017 | 28752804 |
| characterization of microbial communities in chinese rice wine collected at yichang city and suzhou city in china. | two typical microbial communities from chinese rice wine fermentation collected in yichang city and suzhou city in china were investigated. both communities could ferment glutinous rice to rice wine in 2 days. the sugar and ethanol contents were 198.67 and 14.47 mg/g, respectively, for rice wine from yichang city, and 292.50 and 12.31 mg/g, respectively, for rice wine from suzhou city. acetic acid and lactic acid were the most abundant organic acids. abundant fungi and bacteria were detected in ... | 2017 | 28621110 |
| production of fumaric acid by bioconversion of corncob hydrolytes using an improved rhizopus oryzae strain. | the use of microorganism fermentation for production of fumaric acid (fa), which is widely used in food, medicine, and other fields, can provide technical support for the fa industry. in this study, we aimed to increase the titer of fa production by using an improved rhizopus oryzae wht5, which was domesticated to obtain a furfural-resistant strain in corncob hydrolytes. the metabolic pathways and metabolic network of this strain were investigated, and the related enzymes and metabolic flux were ... | 2017 | 28791562 |
| alternaria toxins in south african sunflower seeds: cooperative study. | sunflower seed samples (n = 80) from different sunflower cultivars originating from different localities in south africa were analyzed for 15 toxins produced by fungi of the genus alternaria by means of a simple one-step extraction dilute-and-shoot hplc-ms/ms approach. references for valine-tenuazonic acid (val-tea), altenusin (alts), and altenuisol (altsoh) were isolated from fungal culture extracts and spectroscopically characterized. additionally, valine-tenuazonic acid was tested regarding i ... | 2017 | 28755328 |
| amylolytic fungi in starter cakes for rice beer production. | two types of starter cakes, viz. amou and perok-kushi, used in the production of rice beer in assam, india, by the bodo and deori communities, respectively, were used for the isolation of amylolytic fungi. based on the sequencing of their internal transcribed spacer (its) regions the fungi were identified as amylomyces rouxii and rhizopus oryzae, and given the strain names tu460 and tu465, respectively. both the strains showed the ability to degrade and saccharify starch. the glucoamylase activi ... | 2017 | 28747585 |
| biochemical characterization and stability assessment of rhizopus oryzae lipase covalently immobilized on amino-functionalized magnetic nanoparticles. | amino-functionalized magnetic nanoparticles (fe3o4) have been investigated as a support for covalent immobilization of lipase. the nanoparticles were prepared by chemical coprecipitation method and subsequently were coated with 3-aminopropyltriethoxysilane (aptes) via silanization reaction. with glutaraldehyde, as the coupling agent, the lipase from rhizopus oryzae was successfully immobilized onto the amino-functionalized magnetic nanoparticles. the synthesized support was characterized by tran ... | 2017 | 28711611 |
| exploring substrate specificities of a recombinant rhizopus oryzae lipase in biodiesel synthesis. | the alcoholysis of triolein was used to explore the specific features of a recombinant rhizopus oryzae lipase (rrol) for biodiesel synthesis. for this purpose, different acylglycerols were compared as substrates in lipase-catalysed transesterification. rrol was shown to exhibit a higher specificity towards 1-monoolein than triolein compared to other r. oryzae lipases, being more than 4-fold more specific; in contrast, rrol did not accept 2-monoolein as substrate, concluding that it is highly 1,3 ... | 2017 | 28711520 |
| higher in vitro proliferation rate of rhizopus oryzae in blood of diabetic individuals in chronic glycaemic control compared with non-diabetic individuals. | metabolic control improves outcomes associated with mucormycosis. the aim of this study was to compare the in vitro proliferation of rhizopus oryzae in blood of individuals with and without diabetes at different glycaemic levels. ninety-five individuals were included. blood samples from each participant were incubated with sporangiospores of r. oryzae. the germination, filamentation and growth of r. oryzae were compared at different time points. four groups were defined, one without (group a, n ... | 2017 | 28685375 |
| n-glycosylation engineering to improve the constitutive expression of rhizopus oryzae lipase in komagataella phaffii. | our previous studies demonstrated that the n-glycans in rhizopus chinensis lipase (rcl) was important for its secretion. in order to improve the secretion of rhizopus oryzae lipase (rol) under the control of the gap promoter in komagataella phaffii, two extra n-glycosylation sites were introduced in rol according to the position of the n-glycosylation sites of rcl by sequence alignment. the results indicated that the secretion level of rol was strongly improved by n-glycosylation engineering, an ... | 2017 | 28681607 |
| semi-continuous production of high-activity pectinases by immobilized rhizopus oryzae using tobacco wastewater as substrate and their utilization in the hydrolysis of pectin-containing lignocellulosic biomass at high solid content. | in this study, highly reactive endo- and exo-polygalacturonases (pgs) were produced from the tobacco industry wastewater using immobilized rhizopus oryzae. compared with free cells, immobilized cells increased enzyme activity 2.8-fold and reduced production time to 24h by shake-flask production. moreover, the immobilized cells enabled the semi-continuous production of enzymes through repeated-batch mode for seven consecutive cycles in a scale-up bioreactor. during the first five cycles, the aver ... | 2017 | 28673517 |
| rhizopus oryzae - ancient microbial resource with importance in modern food industry. | filamentous fungi are microorganisms widely known for their diverse biochemical features. fungi can efficiently invade a wide variety of substrates under operational conditions producing numerous bioproducts of interest, such as enzymes, organic acids, aromatic compounds and colorants. an additional interesting characteristic of some fungi is their safety classification for different uses, which guarantees that the bioproducts obtained from them do not contain any toxic component deleterious to ... | 2017 | 28651077 |
| rhizopus homothallicus causing invasive infections: series of three cases from a single centre in north india. | mucormycoses are opportunistic fungal infections with a high mortality rate. rhizopus oryzae is the most common agent implicated in human infections. although r. homothallicus has been previously reported to be a cause of pulmonary mucormycosis, it is the first time that we are reporting as a causative agent of rhino-orbital and cutaneous mucormycosis. | 2017 | 28623532 |
| moderate expression of sec16 increases protein secretion by saccharomyces cerevisiae. | the yeast saccharomyces cerevisiae is widely used to produce biopharmaceutical proteins. however, the limited capacity of the secretory pathway may reduce its productivity. here, we increased the secretion of a heterologous α-amylase, a model protein used for studying the protein secretory pathway in yeast, by moderately overexpressing sec16, which is involved in protein translocation from the endoplasmic reticulum to the golgi apparatus. the moderate overexpression of sec16 increased α-amylase ... | 2017 | 28476767 |
| biodegradation of thermally treated low density polyethylene by fungus rhizopus oryzae ns 5. | polythene is considered as one of the important object used in daily life. being versatile in nature and resistant to microbial attack, they effectively cause environmental pollution. in the present study, biodegradation of low-density polyethylene (ldpe) have been performed using fungal lab isolate rhizopus oryzae ns5. lab isolate fungal strain capable of adhering to ldpe surface was used for the biodegradation of ldpe. this strain was identified as rhizopus oryzae ns5 (accession no. kt160362). ... | 2017 | 28452021 |
| identification of a novel rhizopus-specific antigen by screening with a signal sequence trap and evaluation as a possible diagnostic marker of mucormycosis. | mucormycosis is the second most common mould infection, often indistinguishable from other invasive mould infections such as aspergillosis. although an appropriate antifungal therapy is effective at an early stage of the infection, there is no reliable diagnostic method for decision making. thus, it is necessary to develop an efficient method that can detect mucormycosis rapidly and accurately. we searched for secreted or membrane-bound proteins of rhizopus oryzae, which is the most common patho ... | 2017 | 28199672 |
| short communication: conversion of lactose and whey into lactic acid by engineered yeast. | lactose is often considered an unwanted and wasted byproduct, particularly lactose trapped in acid whey from yogurt production. but using specialized microbial fermentation, the surplus wasted acid whey could be converted into value-added chemicals. the baker's yeast saccharomyces cerevisiae, which is commonly used for industrial fermentation, cannot natively ferment lactose. the present study describes how an engineered s. cerevisiae yeast was constructed to produce lactic acid from purified la ... | 2017 | 27837988 |
| the impact of rhizopus oryzae cultivation on rice bran: gamma-oryzanol recovery and its antioxidant properties. | this study evaluated the effect of the solid state cultivation (ssc) time of rice bran by rhizopus oryzae on γ-oryzanol recovery and its antioxidant properties. gamma-oryzanol was extracted with organic solvents and its extracts were characterized by gc-fid and hplc-uv. the antioxidant capacity was assessed by dpph and abts(+) assays, β-carotene/linoleic acid system, and reduction of oxidation in lipid system. the biomass showed the γ-oryzanol recovery increased by 51.5% (20.52mg/g), and 5.7% in ... | 2017 | 28317745 |
| increased dosage of aox1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in pichia pastoris. | the methanol-regulated alcohol oxidase promoter (paox1) of pichia pastoris is one of the strongest promoters for heterologous gene expression in this methylotrophic yeast. although increasing gene dosage is one of the most common strategies to increase recombinant protein productivities, the increase of gene dosage of rhizopus oryzae lipase (rol) in p. pastoris has been previously shown to reduce cell growth, lipase production and substrate consumption in high-copy strains. to better assess that ... | 2017 | 28295011 |
| improving bread quality with the application of a newly purified thermostable α-amylase from rhizopus oryzae fsis4. | a new thermostable α-amylase from rhizopus oryzae fsis4 was purified for first time and recovered in a single step using a three-phase partitioning (tpp) system. the fungal α-amylase, at a concentration of 1.936 u per kg of flour, was used in bread-making and compared to the commercial enzyme. the results showed a significant effect of the recovered α-amylase in the prepared bread and allowed us to improve the quality of the bread. the study indicated clearly that the recovered α-amylase is a po ... | 2017 | 28231081 |
| hydrolysis of carotenoid esters from tagetes erecta by the action of lipases from yarrowia lipolytica. | the present study was conducted to evaluate the feasibility of enzymatic hydrolysis of carotenoid esters from tagetes erecta using lipases from the yeast of yarrowia lipolytica, with the aim of obtaining free lutein. the optimal concentrations of seven nutrients, considering the production of lipases relative to biomass (yp/x) as the response variable, were determined in flask fermentations. in addition, we studied the effect on hydrolysis of growing y. lipolytica in the presence of the oleoresi ... | 2017 | 28133596 |
| purification and properties of extracellular lipases with transesterification activity and 1,3-regioselectivity from rhizomucor miehei and rhizopus oryzae. | rhizomucor miehei nrrl 5282 and rhizopus oryzae nrrl 1526 can produce lipases with high synthetic activities in wheat bran-based solid-state culture. in this study, the purification and biochemical characterization of the lipolytic activities of these lipases are presented. sds-page indicated a molecular mass of about 55 and 35 kda for the purified r. miehei and rh. oryzae enzymes, respectively. p-nitrophenyl palmitate (pnpp) hydrolysis was maximal at 40°c and ph 7.0 for the r. miehei lipase, an ... | 2017 | 27780957 |
| skin lesion in a patient with acute myeloid leukemia. | we present the case of a 51-year-old man with acute myeloid leukemia who developed fevers with a skin lesion following the first cycle of induction chemotherapy. skin biopsy showed evidence of invasive fungal infection. cultures remained negative, but polymerase chain reaction on tissue detected rhizopus oryzae complex. the patient was started on liposomal amphotericin b and underwent surgical debridement. he was switched to posaconazole, with plans for allogeneic hematopoetic stem cell transpla ... | 2017 | 27862750 |
| fumaric acid production from alkali-pretreated corncob by fed-batch simultaneous saccharification and fermentation combined with separated hydrolysis and fermentation at high solids loading. | production of fumaric acid from alkali-pretreated corncob (apc) at high solids loading was investigated using a combination of separated hydrolysis and fermentation (shf) and fed-batch simultaneous saccharification and fermentation (ssf) by rhizopus oryzae. four different fermentation modes were tested to maximize fumaric acid concentration at high solids loading. the highest concentration of 41.32 g/l fumaric acid was obtained from 20 % (w/v) apc at 38 °c in the combined shf and fed-batch ssf p ... | 2017 | 27604834 |
| primary cutaneous mucormycosis caused by rhizopus oryzae: a case report and review of literature. | mucormycosis is an invasive infection caused by opportunistic fungi. rhizopus, lichtheimia, mucor and rhizomucor are the most common isolated genera. primary cutaneous mucormycosis is usually related to traumatic injuries, but immunocompromised cases are associated with underlying conditions such as diabetes mellitus and malignancies. the treatment of choice is surgical debridement and liposomal amphotericin b. we present a 40-year-old male with fever and a painful necrotic lesion on the middle ... | 2017 | 27807669 |
| engineering rtca pathway and c4-dicarboxylate transporter for l-malic acid production. | l-malic acid is an important component of a vast array of food additives, antioxidants, disincrustants, pharmaceuticals, and cosmetics. here, we presented a pathway optimization strategy and a transporter modification approach to reconstruct the l-malic acid biosynthesis pathway and transport system, respectively. first, pyruvate carboxylase (pyc) and malate dehydrogenase (mdh) from aspergillus flavus and rhizopus oryzae were combinatorially overexpressed to construct the reductive tricarboxylic ... | 2017 | 28229207 |
| matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens. | filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (frs). current laboratory diagnosis of frs pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. although matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms has been employed to identify various fungi, its efficacy in the identification of frs fungi is less clear. | 2017 | 28100314 |
| in vitro and in vivo antifungal profile of a novel and long acting inhaled azole, pc945, on aspergillus fumigatus infection. | the profile of pc945, a novel triazole antifungal, designed for administration via inhalation, has been assessed in a range of in vitro and in vivo studies. pc945 was characterized as a potent, tight-binding inhibitor of aspergillus fumigatus sterol 14α-demethylase (cyp51a and cyp51b) activity (ic50, 0.23 μm and 0.22 μm, respectively), with characteristic type ii azole binding spectra. against 96 clinically isolated a. fumigatus strains, the mic values of pc945 ranged from 0.032∼>8 μg/ml, whilst ... | 2017 | 28223388 |
| effect of magnesium acetate on the antimold activity of lactobacillus. | the antimold activity of lactic acid bacteria (lab) is used in food biopreservation. the aim of this study was to evaluate the effect of magnesium acetate added to de man rogosa sharpe (mrs) medium on the antimold activity of three lab strains ( lactobacillus plantarum , lactobacillus brevis , and lactobacillus fermentum ) against molds contaminating food ( aspergillus oryzae , aspergillus niger , penicillium chrysogenum , fusarium avenaceum , and rhizopus arrhizus ) and their ability to produce ... | 2017 | 28221876 |
| can fungi compete with marine sources for chitosan production? | chitosan, a β-1,4-linked glucosamine polymer is formed by deacetylation of chitin. it has a wide range of applications from agriculture to human health care products. chitosan is commercially produced from shellfish, shrimp waste, crab and lobster processing using strong alkalis at high temperatures for long time periods. the production of chitin and chitosan from fungal sources has gained increased attention in recent years due to potential advantages in terms of homogenous polymer length, high ... | 2017 | 28143744 |
| analysis of microbial diversity in shenqu with different fermentation times by pcr-dgge. | shenqu is a fermented product that is widely used in traditional chinese medicine (tcm) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. the aim of this study was to investigate microbial diversity in shenqu using different fermentation time periods. dgge (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated that a strain of pediococcus acidilactici (band 9) is the predominant bacteria during fermentation and tha ... | 2017 | 28256389 |
| identification of the potent toxin bongkrekic acid in a traditional african beverage linked to a fatal outbreak. | in january 2015, 75 people died and 177 were hospitalized in the mozambique village of chitima after attending a funeral. the deaths were linked to the consumption of a traditional african beverage called pombe. samples of the suspect pombe were subjected to myriad analyses and compared to a control sample. ultimately, non-targeted liquid chromatography-mass spectrometry screening revealed the presence of the potent toxin bongkrekic acid, and its structural isomer, isobongkrekic acid. quantitati ... | 2017 | 27823840 |
| production of flavor compounds from olive mill waste by rhizopus oryzae and candida tropicalis. | the purpose of this study was to investigate the production of flavor compounds from olive mill waste by microbial fermentation of rhizopus oryzae and candida tropicalis. olive mill waste fermentations were performed in shake and bioreactor cultures. production of flavor compounds from olive mill waste was followed by gas chromatography-mass spectrometry, gas chromatography- olfactometry and spectrum sensory analysis(®). as a result, 1.73-log and 3.23-log cfu/ml increases were observed in the mi ... | 2017 | 28017541 |
| preparation of biodiesel with liquid synergetic lipases from rapeseed oil deodorizer distillate. | to reduce industrial production cost, cheap and easily available rapeseed oil deodorizer distillates were used as feedstock to prepare biodiesel in this study. as a result, liquid forms of candida rugosa lipase and rhizopus oryzae lipase (rol) were functioned as new and effective catalysts with biodiesel yield of 92.63% for 30 h and 94.36% for 9 h, respectively. furthermore, the synergetic effect between the two lipases was employed to enhance biodiesel yield with a result of 98.16% in 6 h under ... | 2017 | 28353044 |
| high-throughput analysis of enzymatic hydrolysis of biodegradable polyesters by monitoring cohydrolysis of a polyester-embedded fluorogenic probe. | biodegradable polyesters have the potential to replace nondegradable, persistent polymers in numerous applications and thereby alleviate plastic accumulation in the environment. herein, we present an analytical approach to study enzymatic hydrolysis of polyesters, the key step in their overall biodegradation process. the approach is based on embedding fluorescein dilaurate (fdl), a fluorogenic ester substrate, into the polyester matrix and on monitoring the enzymatic cohydrolysis of fdl to fluor ... | 2017 | 28140581 |
| rhizopus arrhizus and fusarium solani concomitant infection in an immunocompromised host. | neutropenic patients are at risk of the development of hyalohyphomycosis and mucormycosis. correct identification is essential for the initiation of the specific treatment, but concomitant mold infections are rarely reported. we report one unprecedented case of concomitant mucormycosis and fusariosis in a neutropenic patient with acute myeloid leukemia. the patient developed rhino-orbital infection by rhizopus arrhizus and disseminated infection by fusarium solani. the first culture from a sinus ... | 2016 | 26346377 |
| heterologous expression of an active chitin synthase from rhizopus oryzae. | chitin synthases are highly important enzymes in nature, where they synthesize structural components in species belonging to different eukaryotic kingdoms, including kingdom fungi. unfortunately, their structure and the molecular mechanism of synthesis of their microfibrilar product remain largely unknown, probably because no fungal active chitin synthases have been isolated, possibly due to their extreme hydrophobicity. in this study we have turned to the heterologous expression of the transcri ... | 2016 | 27777036 |
| antibacterial effects of biosynthesized silver nanoparticles on surface ultrastructure and nanomechanical properties of gram-negative bacteria viz. escherichia coli and pseudomonas aeruginosa. | understanding the interactions of silver nanoparticles (agnps) with the cell surface is crucial for the evaluation of bactericidal activity and for advanced biomedical and environmental applications. biosynthesis of agnps was carried out through in situ reduction of silver nitrate (agno3) by cell free protein of rhizopus oryzae and the synthesized agnps was characterized by uv-vis spectroscopy, high resolution transmission electron microscopy (hrtem), dynamic light scattering (dls), ζ-potential ... | 2016 | 26829373 |
| reclamation of dpk hydrocarbon polluted agricultural soil using a selected bulking agent. | in the present study, laboratory scale bioremediation of dual purpose kerosene (dpk) hydrocarbon polluted soil using bulking agent (saw dust) was carried out. the effect of different parameters such as total petroleum hydrocarbon (tph), dehydrogenase activity (dhase) and ph on bioremediation performance were evaluated. studied parameters such as microbial dynamics, percentage degradation (95.20%), dhase (8.20 ± 0.43) were found to be higher in saw dust amended system and significantly differed w ... | 2016 | 26934642 |
| fumarate production by torulopsis glabrata: engineering heterologous fumarase expression and improving acid tolerance. | fumarate is a well-known biomass building block compound. however, the poor catalytic efficiency of fumarase is one of the major factors preventing its widespread production. to address this issue, we selected residues 159hpnd162 of fumarase from rhizopus oryzae as targets for site-directed mutagenesis based on molecular docking analysis. twelve mutants were generated and characterized in detail. kinetic studies showed that the km values of the p160a, p160t, p160h, n161e, and d162w mutants were ... | 2016 | 27711153 |
| co-production of fungal biomass derived constituents and ethanol from citrus wastes free sugars without auxiliary nutrients in airlift bioreactor. | the potential of two zygomycetes fungi, mucor indicus and rhizopus oryzae, in assimilating citrus waste free sugars (cwfs) and producing fungal chitosan, oil, and protein as well as ethanol was investigated. extraction of free sugars from citrus waste can reduce its environmental impact by decreasing the possibility of wild microorganisms growth and formation of bad odors, a typical problem facing the citrus industries. a total sugar concentration of 25.1 g/l was obtained by water extraction of ... | 2016 | 26927089 |
| production and optimization of biodiesel using mixed immobilized biocatalysts in packed bed reactor. | vegetable oils are used as raw materials for biodiesel production using transesterification reaction. several methods for the production of biodiesel were developed using chemical (alkali and acidic compounds) and biological catalysts (lipases). biodiesel production catalyzed by lipases is energy and cost-saving processes and is carried out at normal temperature and pressure. the need for an efficient method for screening larger number of variables has led to the adoption of statistical experime ... | 2016 | 25940482 |
| antioxidant and neuroprotective effects of doenjang prepared with rhizopus, pichia, and bacillus. | a new type of doenjang was manufactured by mixing soaked soybean, koji (rhizopus oryzae), cheonggukjang (bacillus amyloliquefaciens mj1-4 and b. amyloliquefaciens emd17), and pichia farinosa sy80 as a yeast, salt, and water, followed by fermentation with koji that was made by fermenting whole wheat with r. oryzae. the mixed culture doenjang was designed to have a more palatable flavor and stronger biological activities than the conventional product. the extract of mixed culture doenjang showed h ... | 2016 | 27752498 |
| immunoreactivity, sensory and physicochemical properties of fermented soy protein isolate. | the effect of induced liquid state fermentation (bacillus subtilis, rhizopus oryzae, saccharomyces cerevisiae, lactobacillus helveticus) on the immunoreactivity, physicochemical and sensory properties of soy protein isolate (spi) was studied. l. helveticus revealed the most abundant reduction in terms of immunoreactivity within soluble protein fractions, up to 100%, which could be measured by in vitro sandwich elisa using mouse monoclonal anti-glym5 antibodies (mabs). almost no binding was found ... | 2016 | 27006235 |
| pleosporalone a, the first azaphilone characterized with aromatic a-ring from a marine-derived pleosporales sp. fungus. | a fungal strain, pleosporales sp. cf09-1, was isolated from marine sediment collected from the bohai sea. a novel azaphilone derivative, named pleosporalone a (1), along with two known analogues, cohaerins a and b (2 and 3), were obtained and identified from the culture extract of pleosporales sp. cf09-1. their planar structures were elucidated by detailed analysis of spectroscopic data and by comparison with related known compounds. pleosporalone a (1) represents the first azaphilone derivative ... | 2016 | 27385435 |
| synthesis, characterization and biological approach of metal chelates of some first row transition metal ions with halogenated bidentate coumarin schiff bases containing n and o donor atoms. | the impregnation of halogen atoms in a molecule is an emerging trend in pharmaceutical chemistry. the presence of halogens (cl, br, i and f) increases the lipophilic nature of molecule and improves the penetration of lipid membrane. the presence of electronegative halogen atoms increases the bio- activity of core moiety. in the present study, co(ii), ni(ii) and cu(ii) complexes are synthesised using schiff bases (hl(i) and hl(ii)), derived from 8-formyl-7-hydroxy-4-methylcoumarin/3-chloro-8-form ... | 2016 | 26874303 |
| stress response in medically important mucorales. | mucorales are saprobes, ubiquitously distributed and able to infect a heterogeneous population of human hosts. the fungi require robust stress responses to survive in human host. we tested the growth of mucorales in the presence of different abiotic stress. eight pathogenic species of mucorales, including rhizopus arrhizus, rhizopus microsporus, rhizomucor pusillus, apophysomyces elegans, licthemia corymbifera, cunninghamella bertholletiae, syncephalastrum racemosum and mucor racemosus, were exp ... | 2016 | 27292160 |
| antifungal activity of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides: a novel type of compound afforded by azide-enolate (3+2) cycloaddition. | the first report of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides (7a and 7b) is described herein. azide-enolate (3+2) cycloaddition afforded the synthesis of this novel type of compound. antifungal activity was evaluated in vitro against four filamentous fungi (aspergillus fumigatus, trichosporon cutaneum, rhizopus oryzae and mucor hiemalis) as well as nine species of candida spp. as yeast specimens. these pre-clinical studies suggest that compounds 7a and 7b are promising candidates for co ... | 2016 | 27656774 |
| enose technology can detect and classify human pathogenic molds in vitro: a proof-of-concept study of aspergillus fumigatus and rhizopus oryzae. | invasive pulmonary mold disease (ipmd) is often fatal in neutropenic patients. this is because ipmd is difficult to diagnose timely, especially when non-aspergillus molds are the causative agent, as they are usually not associated with a positive galactomannan assay. in 2013 we showed that exhaled breath analysis might be used to diagnose invasive aspergillosis through profiling of patterns in exhaled volatile organic compounds (vocs) by electronic nose (enose) technology. the current study aime ... | 2016 | 27447026 |
| acute invasive fungal rhinosinusitis: our experience with 18 cases. | acute invasive fungal rhinosinusitis (aifrs) is a rapidly progressive life threatening infection that is seen most commonly among immunocompromised patients. we present a case series of 18 patients clinically and histopathologically diagnosed with aifrs with a mean follow-up of 9.11 ± 2.51 months (range 6-17). demographic data, apparent symptoms and signs, underlying disorders, and outcomes are discussed. the mean age was 39.56 ± 20.66 years (range 2-75). the most common underlying diseases were ... | 2016 | 27272179 |
| azide-enolate 1,3-dipolar cycloaddition in the synthesis of novel triazole-based miconazole analogues as promising antifungal agents. | seven miconazole analogs involving 1,4,5-tri and 1,5-disubstituted triazole moieties were synthesized by azide-enolate 1,3-dipolar cycloaddition. the antifungal activity of these compounds was evaluated in vitro against four filamentous fungi, including aspergillus fumigatus, trichosporon cutaneum, rhizopus oryzae, and mucor hiemalis as well as three species of candida spp. as yeast specimens. these pre-clinical studies suggest that compounds 4b, 4d and 7b can be considered as drug candidates fo ... | 2016 | 26890112 |
| identification and characterization of haemofungin, a novel antifungal compound that inhibits the final step of haem biosynthesis. | during recent decades, the number of invasive fungal infections among immunosuppressed patients has increased significantly, whereas the number of effective systemic antifungal drugs remains low and unsatisfactory. the aim of this study was to characterize a novel antifungal compound, cw-8/haemofungin, which we previously identified in a screen for compounds affecting fungal cell wall integrity. | 2016 | 26747101 |
| stimulation with lysates of aspergillus terreus, candida krusei and rhizopus oryzae maximizes cross-reactivity of anti-fungal t cells. | invasive fungal diseases caused by filamentous fungi and yeasts are significant causes of morbidity and mortality in immunosuppressed hematology patients. we previously published a method to expand aspergillus fumigatus-specific t cells for clinical cell therapy. in the present study, we investigated expansion of t cells specific for other fungal pathogens and creation of a broadly reactive panfungal t-cell product. | 2016 | 26552765 |
| discrimination of aspergillosis, mucormycosis, fusariosis, and scedosporiosis in formalin-fixed paraffin-embedded tissue specimens by use of multiple real-time quantitative pcr assays. | in a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (ffpe) tissue specimens with histopathology results were tested. two 4- to 5-μm ffpe tissue sections from each specimen were digested with proteinase k, followed by automated nucleic acid extraction. multiple real-time quantitative pcr (qpcr) assays targeting the internal transcribed spacer 2 (its2) region of ribosomal dna, using fluorescently labeled primers, was performed to identify clinically important genera and spec ... | 2016 | 27605714 |
| new insight into microbial diversity and functions in traditional vietnamese alcoholic fermentation. | the roles of microorganisms in traditional alcoholic fermentation are often assumed based on abundance in the starter and activity in pure culture. there is a serious lack of hard evidence on the behavior and activity of individual microbial species during the actual fermentation process. in this study, microbial succession and metabolite changes during 7days of traditional vietnamese alcoholic fermentation were monitored. special attention was devoted to starch degradation. in total, 22 microbi ... | 2016 | 27236224 |
| omics-based approaches reveal phospholipids remodeling of rhizopus oryzae responding to furfural stress for fumaric acid-production from xylose. | in order to relieve the toxicity of furfural on rhizopus oryzae fermentation, the molecular mechanism of r. oryzae responding to furfural stress for fumaric acid-production was investigated by omics-based approaches. in metabolomics analysis, 29 metabolites including amino acid, sugars, polyols and fatty acids showed significant changes for maintaining the basic cell metabolism at the cost of lowering fumaric acid production. to further uncover the survival mechanism, lipidomics was carried out, ... | 2016 | 27697734 |
| utilization of discard bovine bone as a support for immobilization of recombinant rhizopus oryzae lipase expressed in pichia pastoris. | in this study the possibility of using discard bovine bone as support for immobilization of rhizopus oryzae lipase expressed in pichia pastoris was analyzed. discard bovine bone were milled and then subjected to a chemical treatment with acetone in order to remove lipids and blood traces. two types of supports were evaluated: bovine bone and calcined bovine bone for 2 h at 600°c. supports were characterized by: icp, sem, xrd, ftir, xps, and n2 adsorption isotherms. calcined bovine bone presented ... | 2016 | 27339042 |
| comparison of lipases for in vitro models of gastric digestion: lipolysis using two infant formulas as model substrates. | the aim of this study was to find a lipase suitable as a surrogate for human gastric lipase (hgl), since the development of predictive gastrointestinal lipolysis models are hampered by the lack of a lipase with similar digestive properties as hgl. three potential surrogates for hgl; rhizopus oryzae lipase (rol), rabbit gastric lipase (rgl) and recombinant hgl (rhgl), were used to catalyze the in vitro digestion of two infant formulas (a medium-chain triacylglyceride enriched formula (mc-if) and ... | 2016 | 27711870 |
| compositional changes in (iso)flavonoids and estrogenic activity of three edible lupinus species by germination and rhizopus-elicitation. | the effects of germination and elicitation on (iso)flavonoid composition of extracts from three edible lupine species (lupinus luteus, lupinus albus, lupinus angustifolius) were determined by rp-uhplc-ms(n). the total (iso)flavonoid content of lupine increased over 10-fold upon germination, with the total content and composition of isoflavonoids more affected than those of flavonoids. glycosylated isoflavones were the most predominant compounds found in lupine seedlings. lesser amounts of isofla ... | 2016 | 26749476 |
| biodiesel production from nannochloropsis gaditana lipids through transesterification catalyzed by rhizopus oryzae lipase. | biodiesel (fatty acid methyl esters, fames) was produced from saponifiable lipids (sls) extracted from wet nannochloropsis gaditana biomass using methanolysis catalyzed by rhizopus oryzae intracellular lipase. sls were firstly extracted with ethanol to obtain 31 wt% pure sls. but this low sl purity also gave a low biodiesel conversion (58%). this conversion increased up to 80% using sls purified by crystallization in acetone (95 wt% purity). polar lipids play an important role in decreasing the ... | 2016 | 26735878 |
| impacts of lignocellulose-derived inhibitors on l-lactic acid fermentation by rhizopus oryzae. | inhibitors generated in the pretreatment and hydrolysis of corn stover and corn cob were identified. in general, they inhibited cell growth, lactate dehydrogenase, and lactic acid production but with less or no adverse effect on alcohol dehydrogenase and ethanol production in batch fermentation by rhizopus oryzae. furfural and 5-hydroxymethyl furfural (hmf) were highly toxic at 0.5-1 g l(-1), while formic and acetic acids at less than 4 g l(-1) and levulinic acid at 10 g l(-1) were not toxic. am ... | 2016 | 26724548 |
| droplet digital pcr-aided screening and characterization of pichia pastoris multiple gene copy strains. | pichia (syn. komagataella) pastoris is a widely used yeast platform for heterologous protein production. expression cassettes are usually stably integrated into the genome of this host via homologous recombination. although increasing gene dosage is a powerful strategy to improve recombinant protein production, an excess in the number of gene copies often leads to decreased product yields and increased metabolic burden, particularly for secreted proteins. we have constructed a series of strains ... | 2016 | 26704939 |
| in vitro antifungal susceptibility of clinical species belonging to aspergillus genus and rhizopus oryzae. | among filamentous fungal pathogens, aspergillus spp. and zygomycetes account for highest rates of morbidity and mortality among immunocompromised patients. recently developed antifungal drugs offer the potential to improve management and therapeutic outcomes of fungal infections. the aim of this study was to analyse the in vitro activities of voriconazole, itraconazole, amphotericin b and caspofungin against clinical isolates of aspergillus spp. and rhizopus oryzae. | 2016 | 26852191 |
| assay of antioxidant potential of two filamentous fungi isolated from the indonesian fermented dried cassava. | the antioxidant capacity and antioxidant constituents of two filamentous fungi (acremonium charticola and rhizopus oryzae) isolated from the indonesian fermented dried cassava (gathot) were evaluated in the present study. the antioxidant capacity of the fungal crude extracts was assessed based on the 2,2'-azino-bis(3-ethyl-benzthiazolin-6-sulfonicacid) (abts) method. total phenolics were determined based on the folin-ciocalteu method, while the flavonoids content in the fungal extracts was deter ... | 2016 | 26848695 |
| enhancement and modeling of microparticle-added rhizopus oryzae lactic acid production. | lactic acid has a wide industrial application area and can be produced by fungal strains. however, excessive bulk growth form of fungi during the fermentations is a major problem, which limits the fermentation performance. microparticles are excellent tools to prevent bulk fungal growth and provide homogenized fermentation broth to increase uniformity and the prediction performance of the models. therefore, in this study, addition of aluminum oxide and talcum microparticles into fermentations wa ... | 2016 | 26658984 |
| assessing adsorption of polycyclic aromatic hydrocarbons on rhizopus oryzae cell wall components with water-methanol cosolvent model. | the contribution of different fungal cell wall components in adsorption of polycyclic aromatic hydrocarbons (pahs) is still unclear. we isolated rhizopus oryzae cell walls components with sequential extraction, characterized functional groups with nexafs spectra, and determined partition coefficients of pahs on cell walls and cell wall components with cosolvent model. spectra of nexafs indicated that isolated cell walls components were featured with peaks at ~532.7 and ~534.5ev energy. the lipid ... | 2016 | 26655233 |
| enzymatic hydrolysis of polyester thin films: real-time analysis of film mass changes and dissipation dynamics. | cleavage of ester bonds by extracellular microbial hydrolases is considered a key step during the breakdown of biodegradable polyester materials in natural and engineered systems. here we present a novel analytical approach for simultaneous detection of changes in the masses and rigidities of polyester thin films during enzymatic hydrolysis using a quartz crystal microbalance with dissipation monitoring (qcm-d). in experiments with poly(butylene succinate) (pbs) and the lipase of rhizopus oryzae ... | 2016 | 26599203 |
| variation in accumulation of isoflavonoids in phaseoleae seedlings elicited by rhizopus. | seeds from seven species of tribe phaseoleae, i.e. phaseolus, vigna, lablab and psophocarpus, were investigated for inducibility of isoflavonoids by germination with or without subsequent elicitation with rhizopus oryzae. germination alone poorly induced isoflavonoid production (in the range of 0.2-0.7 mg representative compound equivalents (rce)/g dw), whereas application of rhizopus onto the seedlings increased the isoflavonoid content considerably (in the range of 0.5-3.3 mg rce/g dw). the in ... | 2016 | 26593543 |
| cloning and expression of trehalose-6-phosphate synthase 1 from rhizopus oryzae. | trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. in fungi, trehalose-6-phosphate synthase 1 (tps1) plays a key role in the biosynthesis of trehalose. in this study, a full-length cdna from rhizopus oryzae encoding tps1 (designated as rotps1) was isolated. the rotps1 cdna is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8 kda. the amino acid sequence of rotps1 has a relatively high hom ... | 2016 | 26567772 |
| asymmetric bioconversion of acetophenone in nano-sized emulsion using rhizopus oryzae. | the fungal morphologies and pellet sizes were controlled in acetophenone reduction by rhizopus oryzae. the acetophenone conversion and (s)-phenylethanol enantiomeric excesses (e.e.) reached the peak after 72 h of incubation when using pellets with 0.54 mm diameter, which showed an excellent performance compared with suspended mycelia, clumps, and pellets with 0.65 or 0.75 mm diameter. furthermore, nano-sized acetophenone was used as a substrate to improve the performances of biotransformation wo ... | 2016 | 26403817 |