Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| production of zika virus virus-like particles. | zika virus (zikv) is a mosquito-transmitted virus that has caused major outbreaks of disease around the world over the last few years. the infectious zikv consists of a structural protein outer shell surrounding a nucleocapsid. virus-like particles (vlp) consist of the outer structural protein shell, but without the nucleocapsid, and are hence noninfectious. vlp, however, are structurally equivalent to the native virus and thus present a similar antigenic profile. these properties make them good ... | 2021 | 32959245 |
| quality of life of mothers of children with congenital syndrome affected by zika virus. | to investigate how mothers of children with congenital syndrome (microcephaly) associated with zika virus perceive their quality of life. | 2021 | 32844897 |
| an integrated molecular approach to untangling host-vector-pathogen interactions in mosquitoes (diptera: culicidae) from sylvan communities in mexico. | there are ~240 species of culicidae in mexico, of which some are vectors of arthropod-borne viruses such as zika virus, dengue virus, chikungunya virus, and west nile virus. thus, the identification of mosquito feeding preferences is paramount to understanding of vector-host-pathogen interactions that, in turn, can aid the control of disease outbreaks. typically, dna and rna are extracted separately for animal (insects and blood meal hosts) and viral identification, but this study demonstrates t ... | 2021 | 33778029 |
| a bacterially-expressed recombinant envelope protein from usutu virus induces neutralizing antibodies in rabbits. | recently, an emerging flavivirus, usutu virus (usuv), has caused an epidemic among birds in europe, resulting in a massive die-off in eurasian blackbirds. currently found only in europe and africa, it can be envisioned that usutu virus will follow the path of other flaviviruses, like west nile virus and zika virus, and will spread via its mosquito vectors and bird hosts to other parts of the world. several cases of human infections by usutu virus have already been published. anticipating this sp ... | 2021 | 33669414 |
| clinical and preclinical evidence for adverse neurodevelopment after postnatal zika virus infection. | although the zika virus (zikv) typically causes mild or no symptoms in adults, during the 2015-2016 outbreak, zikv infection in pregnancy resulted in a spectrum of diseases in infants, including birth defects and neurodevelopmental disorders identified in childhood. while intense clinical and basic science research has focused on the neurodevelopmental outcomes of prenatal zikv infection, less is known about the consequences of infection during early life. considering the neurotropism of zikv an ... | 2021 | 33445671 |
| transcriptional signatures of zika virus infection in astrocytes. | astrocytes are an early and important target of zika virus (zikv) infection in the developing brain, but the impacts of infection on astrocyte function remain controversial. given that nonhuman primate (nhp) models of zikv infection replicate aspects of neurologic disease seen in human infections, we cultured primary astrocytes from the brain tissue of infant rhesus macaques and then infected the cells with asian or african lineage zikv to identify transcriptional patterns associated with infect ... | 2021 | 33405202 |
| fetal rhesus monkey first trimester zika virus infection impacts cortical development in the second and third trimesters. | zika virus is a teratogen similar to other neurotropic viruses, notably cytomegalovirus and rubella. the goal of these studies was to address the direct impact of zika virus on fetal development by inoculating early gestation fetal rhesus monkeys using an ultrasound-guided approach (intraperitoneal vs. intraventricular). growth and development were monitored across gestation, maternal samples collected, and fetal tissues obtained in the second trimester or near term. although normal growth and a ... | 2021 | 33341889 |
| genome-wide identification of aedes albopictus long noncoding rnas and their association with dengue and zika virus infection. | the asian tiger mosquito, aedes albopictus (ae. albopictus), is an important vector that transmits arboviruses such as dengue (denv), zika (zikv) and chikungunya virus (chikv). long noncoding rnas (lncrnas) are known to regulate various biological processes. knowledge on ae. albopictus lncrnas and their functional role in virus-host interactions are still limited. here, we identified and characterized the lncrnas in the genome of an arbovirus vector, ae. albopictus, and evaluated their potential ... | 2021 | 33481791 |
| the liver x receptor agonist lxr 623 restricts flavivirus replication. | the vector-borne flaviviruses (vbfvs) are well known for causing great misery and death in humans worldwide. the vbfvs include those transmitted by mosquitos, such as zika virus (zikv), dengue virus; and those transmitted by ticks including the tick-borne flavivirus serocomplex and powassan virus (powv). two of our recent reports showed that intracranial powv infection in the reservoir host, peromyscus leucopus, was restricted and caused no overt clinical disease. several modes of analyses sugge ... | 2021 | 34162308 |
| recapitulating zika virus infection in vagina of tree shrew (tupaia belangeri). | sexual transmission of zika virus (zikv) elevates the risk of its dissemination in the female reproductive tract and causes a serious threat to the fetus. however, the available animal models are not appropriate to investigate sexual transmission, dynamics of zikv infection, replication, and shedding. the use of tree shrew as a small animal model of zikv vaginal infection was assessed in this study. a total of 23 sexually mature female tree shrews were infected with zikv gz01 via the intravagina ... | 2021 | 34249779 |
| infection, dissemination, and transmission efficiencies of zika virus in aedes aegypti after serial passage in mosquito or mammalian cell lines or alternating passage in both cell types. | zika virus (zikv) is an arthropod-borne virus (arbovirus) with an urban transmission cycle that primarily involves humans and aedes aegypti. evidence suggests that the evolution of some arboviruses is constrained by their dependency on alternating between disparate (vertebrate and invertebrate) hosts. the goals of this study are to compare the genetic changes that occur in zikv after serial passaging in mosquito or vertebrate cell lines or alternate passaging in both cell types and to compare th ... | 2021 | 34006306 |
| dengue, yellow fever, zika and chikungunya epidemic arboviruses in brazil: ultrastructural aspects. | the impact of arbovirus cocirculation in brazil is unknown. dengue virus (denv) reinfection may result in more intense viraemia or immunopathology, leading to more severe disease. the zika virus (zikv) epidemic in the americas provided pathogenicity evidence that had not been previously observed in flavivirus infections. in contrast to other flaviviruses, electron microscopy studies have shown that zikv may replicate in viroplasm-like structures. flaviviruses produce an ensemble of structurally ... | 2021 | 33566939 |
| subgenomic flavivirus rna (sfrna) associated with asian lineage zika virus identified in three species of ugandan bats (family pteropodidae). | serological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. we hypothesized that prior infection with zikv could be confirmed through the presence of subgenomic flavivirus rna (sfrna) of the 3' untranslated region (utr), which persists in tissues due to xrn-1 stalling during rna decay. we amplified zikv sfrna but not ns5 from three experimentally-infected jamaican fruit bats, sup ... | 2021 | 33863991 |
| detection of virus-related sequences associated with potential etiologies of hepatitis in liver tissue samples from rats, mice, shrews, and bats. | hepatitis is a major global health concern. however, the etiology of 10-20% hepatitis cases remains unclear. some hepatitis-associated viruses, like the hepatitis e virus, are zoonotic pathogens. rats, shrews, and bats are reservoirs for many zoonotic pathogens. therefore, understanding the virome in the liver of these animals is important for the investigation of the etiologies of hepatitis and monitoring the emerging zoonotic viruses. in this study, viral metagenomics and pcr methods were used ... | 2021 | 34177835 |
| systemic inflammation, innate immunity and pathogenesis after zika virus infection in cynomolgus macaques are modulated by strain-specificity within the asian lineage. | zika virus (zikv) is an emerging arbovirus with recent global expansion. historically, zikv infections with asian lineages have been associated with mild disease such as rash and fever. however, recent asian sub-lineages have caused outbreaks in the south pacific and latin america with increased prevalence of neurological disorders in infants and adults. asian sub-lineage differences may partially explain the range of disease severity observed. however, the effect of asian sub-lineage difference ... | 2021 | 34120576 |
| previous exposure to dengue virus is associated with increased zika virus burden at the maternal-fetal interface in rhesus macaques. | concerns have arisen that pre-existing immunity to dengue virus (denv) could enhance zika virus (zikv) disease, due to the homology between zikv and denv and the observation of antibody-dependent enhancement (ade) among denv serotypes. to date, no study has examined the impact of pre-existing denv immunity on zikv pathogenesis during pregnancy in a translational non-human primate model. here we show that macaques with a prior denv-2 exposure had a higher burden of zikv vrna in maternal-fetal int ... | 2021 | 34329306 |
| african-lineage zika virus replication dynamics and maternal-fetal interface infection in pregnant rhesus macaques. | following the zika virus (zikv) outbreak in the americas, zikv was causally associated with microcephaly and a range of neurological and developmental symptoms, termed congenital zika syndrome (czs). the viruses responsible for this outbreak belonged to the asian lineage of zikv. however, in vitro and in vivo studies assessing the pathogenesis of african-lineage zikv demonstrated that african-lineage isolates often replicated to high titers and caused more-severe pathology than asian-lineage iso ... | 2021 | 34076485 |
| a high throughput reporter virus particle microneutralization assay for quantitation of zika virus neutralizing antibodies in multiple species. | zika virus is a flavivirus, transmitted via aedes mosquitos, that causes a range of symptoms including zika congenital syndrome. zika has posed a challenging situation for health, public and economic sectors of affected countries. to quantitate zika virus neutralizing antibody titers in serum samples, we developed a high throughput plate based zika virus reporter virus particle (rvp) assay that uses an infective, non-replicating particle encoding zika virus surface proteins and capsid (cprme) an ... | 2021 | 33891631 |
| zika virus-like particle vaccine protects ag129 mice and rhesus macaques against zika virus. | zika virus (zikv), a mosquito-borne flavivirus, is a re-emerging virus that constitutes a public health threat due to its recent global spread, recurrent outbreaks, and infections that are associated with neurological abnormalities in developing fetuses and guillain-barré syndrome in adults. to date, there are no approved vaccines against zikv infection. various preclinical and clinical development programs are currently ongoing in an effort to bring forward a vaccine for zikv. | 2021 | 33711018 |
| two immunogenic recombinant protein vaccine candidates showed disparate protective efficacy against zika virus infection in rhesus macaques. | zika virus (zikv) infection has caused major public health problems recently. to develop subunit vaccines for zikv, we have previously constructed recombinant zikv envelope protein domain iii (ediii), and the entire ectodomain (e80, which comprises edi, edii and ediii), as vaccine candidates and showed both of them being immunogenic and protective in murine models. in this follow-up study, we compared these vaccine candidates in non-human primates. both of them elicited neutralizing antibody res ... | 2021 | 33451779 |
| early embryonic loss following intravaginal zika virus challenge in rhesus macaques. | zika virus (zikv) is an arthropod-borne virus (arbovirus) and is primarily transmitted by aedes species mosquitoes; however, zikv can also be sexually transmitted. during the initial epidemic and in places where zikv is now considered endemic, it is difficult to disentangle the risks and contributions of sexual versus vector-borne transmission to adverse pregnancy outcomes. to examine the potential impact of sexual transmission of zikv on pregnancy outcome, we challenged three rhesus macaques (m ... | 2021 | 34079560 |
| single amino acid mutations affect zika virus replication in vitro and virulence in vivo. | the 2014-2016 zika virus (zikv) epidemic in the americas resulted in large deposits of next-generation sequencing data from clinical samples. this resource was mined to identify emerging mutations and trends in mutations as the outbreak progressed over time. information on transmission dynamics, prevalence, and persistence of intra-host mutants, and the position of a mutation on a protein were then used to prioritize 544 reported mutations based on their ability to impact zikv phenotype. using t ... | 2020 | 33198111 |
| low aedes aegypti vector competence for zika virus from viremic rhesus macaques. | despite worldwide efforts to understand the transmission dynamics of zika virus (zikv), scanty evaluation has been made on the vector competence of aedes aegypti fed directly on viremic human and non-human primates (nhps). we blood-fed ae. aegypti from two districts in rio de janeiro on six zikv infected pregnant rhesus macaques at several time points, half of which were treated with sofosbuvir (sof). mosquitoes were analyzed for vector competence after 3, 7 and 14 days of incubation. although v ... | 2020 | 33255150 |
| biological characteristics and patterns of codon usage evolution for the african genotype zika virus. | we investigated temporal trends of codon usage changes for different host species to determine their importance in zika virus (zikv) evolution. viral spillover resulting from the potential of codon adaptation to host genome was also assessed for the african genotype zikv in comparison to the asian genotype. to improve our understanding on its zoonotic maintenance, we evaluated in vitro the biological properties of the african genotype zikv in vertebrate and mosquito cell lines. analyses were per ... | 2020 | 33202554 |
| novel hepaci- and pegi-like viruses in native australian wildlife and non-human primates. | the flaviviridae family of positive-sense rna viruses contains important pathogens of humans and other animals, including zika virus, dengue virus, and hepatitis c virus. the flaviviridae are currently divided into four genera-hepacivirus, pegivirus, pestivirus, and flavivirus-each with a diverse host range. members of the genus hepacivirus are associated with an array of animal species, including humans, non-human primates, other mammalian species, as well as birds and fish, while the closely r ... | 2020 | 33240526 |
| zika virus mucosal infection provides protective immunity. | zika virus (zikv) is a major human pathogen. zikv can replicate in female and male reproductive organs, thus facilitating the human-human transmission cycle. viral shedding in the semen can increase the risk of zikv transmission through sexual mode. therefore, the vaginal and anorectal mucosa are relevant sites for zikv infection. however, the pathobiology of zikv transmission through the rectal route is not well understood. here, we utilize a mouse model system to investigate the immunopatholog ... | 2020 | 32051274 |
| zika virus infection. | 2020 | 32047340 | |
| intrinsically disordered protein domains in flavivirus infection. | intrinsically disordered protein regions are at the core of biological processes and involved in key protein-ligand interactions. the flavivirus proteins, of viruses of great biomedical importance such as zika and dengue viruses, exemplify this. several proteins of these viruses have disordered regions that are of the utmost importance for biological activity. disordered proteins can adopt several conformations, each able to interact with and/or bind to different ligands. in fact, such interacti ... | 2020 | 32045581 |
| assessment of contraceptive needs and improving access in the u.s.-affiliated pacific islands in the context of zika. | scientific evidence demonstrated a causal relationship between zika virus infection during pregnancy and neurologic abnormalities and other congenital defects. the u.s. government's zika virus disease contingency response plan recognized the importance of preventing unintended pregnancy through access to high-quality family planning services as a primary strategy to reduce adverse zika-related birth outcomes during the 2016-2017 zika virus outbreak. the u.s.-affiliated pacific islands (usapi) in ... | 2020 | 32045325 |
| a serological point-of-care test for zika virus detection and infection surveillance using an enzyme-free vial immunosensor with a smartphone. | zika virus (zikv) is a mosquito-borne flavivirus (flav) that emerged in brazil in 2015 and has rapidly spread to more than 50 countries worldwide. however, early, accurate, and specific point-of-care (poc) diagnosis of zikv is very difficult because most infected patients are asymptomatic or display nonspecific symptoms similar to those of other viral infections, and most of the analysis also requires instruments. herein, an instrument-free zikv poc test using a drop of blood comprising a vial i ... | 2020 | 31999574 |
| oropouche infection a neglected arbovirus in patients with acute febrile illness from the peruvian coast. | to evaluate the frequency of infection caused by the oropouche virus (orov) in 496 patients with acute febrile disease (afi), whose samples were obtained for the analysis of endemic arboviruses in a previous investigation carried out in 2016. | 2020 | 32041646 |
| zika virus and host interactions: from the bench to the bedside and beyond. | before the emergence of sars-cov-2 (severe acute respiratory syndrome coronavirus 2), the causative agent of the current covid-19 (coronavirus disease 2019) pandemic [...]. | 2020 | 33198165 |
| zika virus and its implications on cord blood banking and transplantation. | umbilical cord blood is an important cellular therapy product used for hematopoietic stem cell transplantation, but the us food and drug administration guidance regarding donor screening to reduce the risk of zika transmission has decreased the number of licensed, eligible cord blood units available for transplantation. there is a crucial need for updated travel risk assessment for zika virus transmission, validated screening tests for zika virus in umbilical cord blood, and further research on ... | 2020 | 32208533 |
| cemeteries in miami-dade county, florida are important areas to be targeted in mosquito management and control efforts. | definable habitats at the neighborhood level provide a wide range of favorable habitats with optimal conditions and environmental resources for mosquito survival. problematic habitats for controlling mosquitoes in urban environments such as tire shops, bromeliad patches, and construction sites must be taken into consideration in the development of effective mosquito management and control in urban areas. cemeteries are often located in highly urbanized areas serving as a haven for populations of ... | 2020 | 32208462 |
| zika virus infection in pregnant women in french guiana: more precarious-more at risk. | a recent study in french guiana suggested that populations living in precarious neighborhoods were more at risk for chikungunya chikv than those living in more privileged areas. the objective of the present study was to test the hypothesis that zika virus (zikv) infection was more frequent in precarious pregnant women than in non-precarious pregnant women, as reflected by their health insurance status. | 2020 | 32208419 |
| comparison of clinical presentation and out-comes of chikungunya and dengue virus infections in patients with acute undifferentiated febrile illness from the sindh region of pakistan. | arboviruses are a cause of acute febrile illness and outbreaks worldwide. recent outbreaks of chikungunya virus (chikv) in dengue endemic areas have alarmed clinicians as unique clinical features differentiating chikv from dengue virus (denv) are limited. this has complicated diagnostic efforts especially in resource limited countries where lab testing is not easily available. therefore, it is essential to analyse and compare clinical features of laboratory confirmed cases to assist clinicians i ... | 2020 | 32203509 |
| codelivery of improved immune complex and virus-like particle vaccines containing zika virus envelope domain iii synergistically enhances immunogenicity. | zika virus (zikv) reemergence poses a significant health threat especially due to its risks to fetal development, necessitating safe and effective vaccines that can protect pregnant women. zika envelope domain iii (ze3) has been identified as a safe and effective vaccine candidate, however it is poorly immunogenic. we previously showed that plant-made recombinant immune complex (ric) vaccines are a robust platform to improve the immunogenicity of weak antigens. in this study, we altered the anti ... | 2020 | 32173095 |
| congenital infections in hong kong: an overview of torch. | congenital infections refer to a group of perinatal infections that may have similar clinical presentations, including rash and ocular findings. torch is the acronym that covers these infections (toxoplasmosis, other [syphilis], rubella, cytomegalovirus, herpes simplex virus). there are, however, other important causes of intrauterine/perinatal infections, including enteroviruses, varicella zoster virus, zika virus, and parvovirus b19. intrauterine and perinatal infections are significant causes ... | 2020 | 32245914 |
| quantitative and selective surface plasmon resonance response based on a reduced graphene oxide-polyamidoamine nanocomposite for detection of dengue virus e-proteins. | dengue viral infection is one of the most common deadliest diseases and has become a recurrent issue for public health in tropical countries. although the spectrum of clinical diagnosis and treatment have recently been established, the efficient and rapid detection of dengue virus (denv) during viremia and the early febrile phase is still a great challenge. in this study, a dithiobis (succinimidyl undecanoate, dsu)/amine-functionalized reduced graphene oxide--polyamidoamine dendrimer (dsu/amine- ... | 2020 | 32245185 |
| a public health systems view of risk communication about zika. | the spread of zika virus throughout latin america and parts of the united states in 2016 and 2017 presented a challenge to public health communicators. the objective of our study was to describe emergency risk communication practices during the 2016-2017 zika outbreak to inform future infectious disease communication efforts. | 2020 | 32243762 |
| serologic surveillance of maternal zika infection in a prospective cohort in leon, nicaragua during the peak of the zika epidemic. | zika virus caused thousands of congenital anomalies during a recent epidemic. because zika emerged in areas endemic for dengue and these related flaviviruses elicit cross-reactive antibodies, it is challenging to serologically monitor pregnant women for zika infection. | 2020 | 32243482 |
| neurological outcomes of congenital zika syndrome in toddlers and preschoolers: a case series. | congenital zika syndrome causes a spectrum of neurological symptoms with varying effects on function that require different therapeutic strategies. to date, this spectrum of effects and its clinical implications have not been completely described. we describe the neurological examination findings in toddlers and preschoolers, including predominant symptom complexes and comorbidities. | 2020 | 32199080 |
| the developmental spectrum of prenatal zika virus exposure. | 2020 | 32199079 | |
| assessment of contraceptive use in puerto rico during the 2016 zika virus outbreak. | the objectives of this analysis were to 1) estimate prevalence of contraceptive use among women at risk for unintended pregnancy and 2) identify correlates of contraceptive use among women with ongoing or potential need for contraceptive services in puerto rico during the 2016 zika virus (zikv) outbreak. | 2020 | 32194040 |
| experience in diagnosing congenital zika syndrome in brazilian children born to asymptomatic mothers. | typical symptoms of primary zika virus infection are not specific and share similarities with other arbovirus infections such as dengue fever and chikungunya. as acute infection can be asymptomatic in up to 73% of cases, infants with microcephaly represent a diagnostic challenge for pediatricians. we describe the frequency of congenital zika syndrome (czs) in brazilian children born to asymptomatic pregnant mothers and its differential diagnosis. | 2020 | 32165128 |
| primary and secondary dengue virus infections elicit similar memory b-cell responses, but breadth to other serotypes and cross-reactivity to zika virus is higher in secondary dengue. | the 4 antigenically distinct serotypes of dengue virus (denv) share extensive homology with each other and with the closely related zika flavivirus (zikv). the development of polyclonal memory b cells (mbcs) to the 4 denv serotypes and zikv during denv infection is not fully understood. | 2020 | 32193549 |
| imported arbovirus infections in spain, 2009-2018. | to determine the epidemiologic and clinical characteristics of patients in spain with imported arbovirus infections, we analyzed 22,655 records from a collaborative network for january 2009-december 2018. among 861 arbovirus infections, 845 were monoinfections (456 [53%] dengue, 280 [32.5%] chikungunya, 109 [12.7%] zika) and 16 (1.8%) were co-infections. most patients were travelers (56.3%) or immigrants returning to spain after visiting friends or relatives (31.3%). median patient age was 37 ye ... | 2020 | 32186486 |
| vector-borne transmission of the zika virus asian genotype in europe. | three autochthonous cases of zika virus occurred in southern france in august 2019. diagnosis relied on serology and transcription-mediated amplification. attempts for virus isolation and zikv genome rt-pcr detection remained negative. since the index case was not identified, we addressed the issue of genotyping and geographical origin by performing hemi-nested pcr and sequencing in the pr gene. analysis of 16 genotype-specific single nucleotides polymorphisms identified the asian genotype and s ... | 2020 | 32182748 |
| topology, antiviral functional residues and mechanism of ifitm1. | interferon-inducible transmembrane proteins (ifitm1/2/3) have been reported to suppress the entry of a wide range of viruses. however, their antiviral functional residues and specific mechanisms are still unclear. here, we firstly resolved the topology of ifitm1 on the plasma membrane where n-terminus points into the cytoplasm and c-terminus resides extracellularly. further, krrk basic residues of ifitm1 locating at 62-67 of the conserved intracellular loop (cil) were found to play a key role in ... | 2020 | 32182730 |
| first report of aedes albopictus infected by dengue and zika virus in a rural outbreak in brazil. | in brazil, dengue (denv) and zika (zikv) viruses are reported as being transmitted exclusively by aedes aegypti in urban settings. this study established the vectors and viruses involved in an arbovirus outbreak that occurred in 2019 in a rural area of espírito santo state, brazil. mosquitoes collected were morphologically identified, sorted in samples, and submitted to molecular analysis for arboviruses detection. phylogenetic reconstruction was performed for the viral sequence obtained. all 39 ... | 2020 | 32163449 |
| the global scientific research response to the public health emergency of zika virus infection. | science studies have been a field of research for different knowledge areas, and they have been successfully used to analyse the construction of scientific knowledge, practice and dissemination. in this study, we aimed to verify how the zika epidemic has moulded the scientific articles published worldwide by analysing international collaborations and the knowledge landscape through time, as well as research topics and country involvement. | 2020 | 32163439 |
| the cyanobacterial saxitoxin exacerbates neural cell death and brain malformations induced by zika virus. | the northeast (ne) region of brazil commonly goes through drought periods, which favor cyanobacterial blooms, capable of producing neurotoxins with implications for human and animal health. the most severe dry spell in the history of brazil occurred between 2012 and 2016. coincidently, the highest incidence of microcephaly associated with the zika virus (zikv) outbreak took place in the ne region of brazil during the same years. in this work, we tested the hypothesis that saxitoxin (stx), a neur ... | 2020 | 32163415 |
| the impact of news exposure on collective attention in the united states during the 2016 zika epidemic. | in recent years, many studies have drawn attention to the important role of collective awareness and human behaviour during epidemic outbreaks. a number of modelling efforts have investigated the interaction between the disease transmission dynamics and human behaviour change mediated by news coverage and by information spreading in the population. yet, given the scarcity of data on public awareness during an epidemic, few studies have relied on empirical data. here, we use fine-grained, geo-ref ... | 2020 | 32163409 |
| computational methodology for peptide vaccine design for zika virus: a bioinformatics approach. | with the increasing frequency of viral epidemics, vaccines to augment the human immune response system have been the medium of choice to combat viral infections. the tragic consequences of the zika virus pandemic in south and central america a few years ago brought the issues into sharper focus. while traditional vaccine development is time-consuming and expensive, recent advances in information technology, immunoinformatics, genetics, bioinformatics, and related sciences have opened the doors t ... | 2020 | 32162248 |
| flavivirus envelope protein glycosylation: impacts on viral infection and pathogenesis. | flaviviruses encode one, two, or no n-linked glycosylation sites on their envelope proteins. glycosylation can impact virus interactions with cell surface attachment factors and also may impact virion stability and virus replication. envelope protein glycosylation has been identified as a virulence determinant for multiple flaviviruses, but the mechanisms by which glycosylation mediates pathogenesis remain unclear. in this gem, we summarize current knowledge on flavivirus envelope protein glycos ... | 2020 | 32161171 |
| a review on zika virus outbreak, epidemiology, transmission and infection dynamics. | zika virus (zikv) is a newly emergent relative of the flaviviridae family and linked to dengue (denv) and chikungunya (chivkv). zikv is one of the rising pathogens promptly surpassing geographical borders. zikv infection was characterized by mild disease with fever, headache, rash, arthralgia and conjunctivitis, with exceptional reports of an association with guillain-barre syndrome (gbs) and microcephaly. however, since the end of 2015, an increase in the number of gbs associated cases and an a ... | 2020 | 32158705 |
| harnessing dengue rapid diagnostic tests for the combined surveillance of dengue, zika, and chikungunya viruses in laos. | recent expansions of vector-borne diseases highlight the need for improved surveillance, especially in resource-poor settings. dengue virus (denv), chikungunya virus (chikv), and zika virus (zikv) share the same vectors as well as similar clinical presentations, suggesting that combined surveillance would be useful. we hypothesized that blood spotted on dengue rapid diagnostic tests (rdts) could be harnessed for sample collection in remote areas for subsequent detection of denv, chikv, and zikv ... | 2020 | 32157991 |
| the mechanism of the zika virus crossing the placental barrier and the blood-brain barrier. | zika virus (zikv) infection causes severe neurological symptoms in adults and fetal microcephaly and the virus is detected in the brain of microcephaly and meningoencephalitis patient. however, the mechanism of zikv crossing the physiological barrier to the central nervous systems (cns) remains elusive. the placental barrier and the blood brain barrier (bbb) protect the fetus from pathogens and ensure healthy brain development during pregnancy. in this study, we used human placenta trophoblasts ... | 2020 | 32153526 |
| adequate placental sampling for the diagnosis and characterization of placental infection by zika virus. | the detection of zika virus (zikv) in immunoprivileged anatomical sites, potential sites for viral persistence, may guide the confirmation of undefined cases of zikv infection and also bring to light unknown pathways of viral transmission. thus, this study aimed to characterize zikv infection in stratified, standardized placental samples in women with exanthematic febrile manifestations during pregnancy and compare findings to the standard investigation protocol of official health agencies. to t ... | 2020 | 32153521 |
| zika virus non-structural protein ns4a restricts eye growth in drosophila through regulation of jak/stat signaling. | to gain a comprehensive view of the changes in host gene expression underlying zika virus (zikv) pathogenesis, we performed whole-genome rna sequencing (rna-seq) of zikv-infected drosophila adult flies. rna-seq analysis revealed that zikv infection alters several and diverse biological processes, including stress, locomotion, lipid metabolism, imaginal disc morphogenesis and regulation of jak/stat signaling. to explore the interaction between zikv infection and jak/stat signaling regulation, we ... | 2020 | 32152180 |
| the broad spectrum antiviral ivermectin targets the host nuclear transport importin α/β1 heterodimer. | infection by rna viruses such as human immunodeficiency virus (hiv)-1, influenza, and dengue virus (denv) represent a major burden for human health worldwide. although rna viruses replicate in the infected host cell cytoplasm, the nucleus is central to key stages of the infectious cycle of hiv-1 and influenza, and an important target of denv nonstructural protein 5 (ns5) in limiting the host antiviral response. we previously identified the small molecule ivermectin as an inhibitor of hiv-1 integ ... | 2020 | 32135219 |
| strong cd4 t cell responses to zika virus antigens in a cohort of dengue virus immune mothers of congenital zika virus syndrome infants. | background: there is an urgent need to understand the complex relationship between cross-reactive anti-viral immunity, disease susceptibility, and severity in the face of differential exposure to related, circulating flaviviruses. co-exposure to dengue virus and zika virus in brazil is a case in point. a devastating aspect of the 2015-2016 south american zika outbreak was the dramatic increase in numbers of infants born with microcephaly to mothers exposed to zika virus during pregnancy. it has ... | 2020 | 32132999 |
| screening and exclusion of zika virus infection in travellers by an ns1-based elisa and qrt-pcr. | zika virus (zikv) infection during pregnancy may cause neurological abnormalities in the foetus, and therefore fast and accurate laboratory assays are critical for rapid diagnosis. elisa based on zikv ns1 protein has been developed and shown to be sensitive and highly specific; however, its negative and positive predictive values have not been tested. in this study we evaluated the ability of the ns1-based elisa to exclude zikv infection and serve as a first-line screening tool for travellers. | 2020 | 32151598 |
| modelling the effective reproduction number of vector-borne diseases: the yellow fever outbreak in luanda, angola 2015-2016 as an example. | the burden of vector-borne diseases (dengue, zika virus, yellow fever, etc.) gradually increased in the past decade across the globe. mathematical modelling on infectious diseases helps to study the transmission dynamics of the pathogens. theoretically, the diseases can be controlled and eventually eradicated by maintaining the effective reproduction number, ( reff ), strictly less than 1. we established a vector-host compartmental model, and derived ( reff ) for vector-borne diseases. the analy ... | 2020 | 32149023 |
| zika, dengue, chikungunya and yellow fever infections in europe? - winter is over, warm days are coming - so hedge your bets. | 2020 | 32145385 | |
| zika virus infection in chemosensory cells. | zika virus (zikv) is an emerging virus belonging to the genus flavivirus. zikv infection is a significant health concern, with increasing numbers of reports of microcephaly cases in fetuses and guillain-barré syndrome (gbs) in adults. interestingly, chemosensory disturbances are also reported as one of the manifestations of gbs. zikv infects several human tissues and cell types in vitro and in vivo. however, there is no study demonstrating zikv infection and replication in chemosensory cells, in ... | 2020 | 32144727 |
| maternal and congenital infections arising from zika, dengue and chikungunya arboviruses in salvador, brazil. | salvador was one of the brazilian cities most affected during the 2015 zika virus (zikv) outbreak. | 2020 | 32142153 |
| ire1α promotes zika virus infection via xbp1. | zika virus (zikv) is an emergent member of the flaviviridae family which causes severe congenital defects and other major sequelae, but the cellular processes that support zikv replication are incompletely understood. related flaviviruses use the endoplasmic reticulum (er) as a membranous platform for viral replication and induce er stress during infection. our data suggest that zikv activates ire1α, a component of the cellular response to er stress. ire1α is an er-resident transmembrane protein ... | 2020 | 32138181 |
| virus like particles (vlp) as multivalent vaccine candidate against chikungunya, japanese encephalitis, yellow fever and zika virus. | mosquito borne viral diseases are an emerging threat as evident from the recent outbreak of zika virus (zikv) as well as repeated outbreaks of chikungunya (chikv), yellow fever (yfv) and japanese encephalitis (jev) virus in different geographical regions. these four arboviruses are endemic in overlapping regions due to the co-prevalence of the transmitting mosquito vector species aedes and culex. thus, a multivalent vaccine that targets all four viruses would be of benefit to regions of the worl ... | 2020 | 32132648 |
| very-long-chain fatty acid metabolic capacity of 17-beta-hydroxysteroid dehydrogenase type 12 (hsd17b12) promotes replication of hepatitis c virus and related flaviviruses. | flaviviridae infections represent a major global health burden. by deciphering mechanistic aspects of hepatitis c virus (hcv)-host interactions, one could discover common strategy for inhibiting the replication of related flaviviruses. by elucidating the hcv interactome, we identified the 17-beta-hydroxysteroid dehydrogenase type 12 (hsd17b12) as a human hub of the very-long-chain fatty acid (vlcfa) synthesis pathway and core interactor. here we show that hsd17b12 knockdown (kd) impairs hcv repl ... | 2020 | 32132633 |
| single cell immune profiling of dengue virus patients reveals intact immune responses to zika virus with enrichment of innate immune signatures. | the genus flavivirus contains many mosquito-borne human pathogens of global epidemiological importance such as dengue virus, west nile virus, and zika virus, which has recently emerged at epidemic levels. infections with these viruses result in divergent clinical outcomes ranging from asymptomatic to fatal. myriad factors influence infection severity including exposure, immune status and pathogen/host genetics. furthermore, pre-existing infection may skew immune pathways or divert immune resourc ... | 2020 | 32150565 |
| c19orf66 interrupts zika virus replication by inducing lysosomal degradation of viral ns3. | the rapidly emerging human health crisis associated with the zika virus (zikv) epidemic and its link to severe complications highlights the growing need to identify the mechanisms by which zikv accesses hosts. interferon response protects host cells against viral infection, while the cellular factors that mediate this defense are the products of interferon-stimulated genes (isgs). although hundreds of isgs have been identified, only a few have been characterized for their antiviral potential, ta ... | 2020 | 32150556 |
| community perspectives on zika virus disease prevention in guatemala: a qualitative study. | zika virus, which is transmitted by aedes aegypti mosquitoes and through sexual transmission, disproportionally affects the human fetus. guatemala experienced a surge of zika cases beginning in 2016. we conducted a qualitative study of community perceptions of the seriousness of zika, as well as the effectiveness, feasibility, and collective efficacy of zika prevention actions. free listing elicited the preventive actions salient for 68 participants comprising pregnant women, men with a pregnant ... | 2020 | 32100677 |
| optimization of recombinant zika virus ns1 protein secretion from hek293 cells. | sensitive, accurate and cost-effective diagnostic tests are urgently needed to detect zika virus (zikv) infection. nonstructural 1 (ns1) glycoprotein is an excellent diagnostic marker since it is released in a hexameric conformation from infected cells into the patient's bloodstream early in the course of the infection. we established a stable rzns1-his-expression system in hek293 cells through lentiviral transduction. a novel optimization approach to enhance rzns1-his protein secretion in the m ... | 2020 | 32095434 |
| correction: zika virus-derived e-diii protein displayed on immunologically optimized vlps induces neutralizing antibodies without causing enhancement of dengue virus infection. vaccines 2019, 7, 72. | the authors wish to make the following correction to their paper [...]. | 2020 | 32093400 |
| adjacent dimer epitope of envelope protein as an important region for zika virus serum neutralization: a computational investigation. | the recent emergence of zika virus (zikv) has affected many countries, with severe clinical manifestations such as fetal microcephaly and guillain-barré syndrome. however, even though it is a major public health concern, there is no approved treatment available. structural knowledge of the main neutralization regions of the envelope (e) protein of zikv and its interactions with neutralizing antibodies (nabs) are crucial for the rational development of subunit vaccines and establishment of antibo ... | 2020 | 32090677 |
| axonal variants of guillain-barré syndrome: an update. | axonal variants of guillain-barré syndrome (gbs) mainly include acute motor axonal neuropathy, acute motor and sensory axonal neuropathy, and pharyngeal-cervical-brachial weakness. molecular mimicry of human gangliosides by a pathogen's lipooligosaccharides is a well-established mechanism for campylobacter jejuni-associated gbs. new triggers of the axonal variants of gbs (axonal gbs), such as zika virus, hepatitis viruses, intravenous administration of ganglioside, vaccination, and surgery, are ... | 2020 | 32140865 |
| ocular manifestations in congenital zika syndrome: about a case of torpedo maculopathy. | to describe pertinent imaging studies and clinical features of a torpedo maculopathy presumably associated with congenital zika syndrome. | 2020 | 32140614 |
| strain-dependent activity of zika virus and exposure history in serological diagnostics. | zika virus (zikv) circulates as two separate lineages, with significant genetic variability between strains. strain-dependent activity has been reported for dengue virus, herpes simplex virus and influenza. strain-dependent activity of subject specimens to a virus could be an impediment to serological diagnosis and vaccine development. in order to determine whether zikv exhibits strain-dependent activity when exposed to antibodies, we measured the neutralizing properties of polyclonal serum and ... | 2020 | 32138262 |
| zika virus infection studies with cd34+ hematopoietic and megakaryocyte-erythroid progenitors, red blood cells and platelets. | to date, several cases of transfusion-transmitted zikv infections have been confirmed. multiple studies detected prolonged occurrence of zikv viral rna in whole blood as compared to plasma samples indicating potential zikv interaction with hematopoietic cells. also, infection of cells from the granulocyte/macrophage lineage has been demonstrated. patients may develop severe thrombocytopenia, microcytic anemia, and a fatal course of disease occurred in a patient with sickle cell anemia suggesting ... | 2020 | 32086956 |
| amp-activated protein kinase restricts zika virus replication in endothelial cells by potentiating innate antiviral responses and inhibiting glycolysis. | viruses are known to perturb host cellular metabolism to enable their replication and spread. however, little is known about the interactions between zika virus (zikv) infection and host metabolism. using primary human retinal vascular endothelial cells and an established human endothelial cell line, we investigated the role of amp-activated protein kinase (ampk), a master regulator of energy metabolism, in response to zikv challenge. zikv infection caused a time-dependent reduction in the activ ... | 2020 | 32086387 |
| researching zika in pregnancy: lessons for global preparedness. | our understanding of congenital infections is based on prospective studies of women infected during pregnancy. the eu has funded three consortia to study zika virus, each including a prospective study of pregnant women. another multi-centre study has been funded by the us national institutes of health. this personal view describes the study designs required to research zika virus, and questions whether funding academics in the eu and usa to work with collaborators in outbreak areas is an effecti ... | 2020 | 32085848 |
| a novel neutralizing antibody targeting a unique cross-reactive epitope on the hi loop of domain ii of the envelope protein protects mice against duck tembusu virus. | the flavivirus e protein induces protective immunity, and its abs cause serious problems for serodiagnosis because of the difficulty in differentiating cross-reactive abs. moreover, cross-reactive abs may increase disease severity after secondary flavivirus infections via ab-dependent enhancement. cross-reactive epitopes are therefore critical for understanding serodiagnosis and improving the general knowledge of flavivirus infections. a minimal epitope, 227gssagtwqn235, was identified by a neut ... | 2020 | 32132180 |
| zika virus infection in the genital tract of non-pregnant females: a systematic review. | this review provides a general overview on the positivity and persistence of zika virus (zikv) in female genital tract (fgt) of non-pregnant women and animals, as well as in cell cultures, and its influence on fgt health. we performed a systematic review based on the prisma statement to identify studies focused on "zika virus" and "non-pregnant female" in pubmed, embase, scopus scholar and web of knowledge databases of full-text papers and abstracts published in english, with no restrictions reg ... | 2020 | 32130356 |
| neurodevelopment of children exposed intra-uterus by zika virus: a case series. | the main goal of this manuscript was to investigate the neurodevelopment of children exposed by zika virus in the intrauterine period who are asymptomatic at birth. newborns with documented zika virus exposure during the intrauterine period who were asymptomatic at birth were followed in the first two years of life for neurodevelopment using bayley iii test. children were classified as having normal or delayed neurodevelopment for age based on most recent bayley iii evaluation results. eighty-fo ... | 2020 | 32109947 |
| neuroimaging findings in congenital zika virus syndrome. | 2020 | 32107965 | |
| characterization of a species e adenovirus vector as a zika virus vaccine. | the development of a safe and efficacious zika virus (zikv) vaccine remains a global health priority. in our previous work, we developed an adenovirus vectored zikv vaccine using a low-seroprevalent human adenovirus type 4 (ad4-prm-e) and compared it to an ad5 vector (ad5-prm-e). we found that vaccination with ad4-prm-e leads to the development of a strong anti-zikv t-cell response without eliciting significant anti-zikv antibodies, while vaccination with ad5-prm-e leads to the development of bo ... | 2020 | 32107394 |
| evaluation of a novel microfluidic immuno-magnetic agglutination assay method for detection of dengue virus ns1 antigen. | dengue virus (denv) is the most important arbovirus worldwide, causing infections in endemic countries and returning travellers from these areas. rapid diagnostic tests are needed to improve patient management and monitor local transmission. the detection of denv non-structural protein 1 (ns1) is a useful tool for the diagnosis, but the currently available methods can be time consuming or lack sensitivity. the objective of our study was to evaluate a new rapid and semi-quantitative microfluidic ... | 2020 | 32069280 |
| zika virus-spread, epidemiology, genome, transmission cycle, clinical manifestation, associated challenges, vaccine and antiviral drug development. | zika virus (zikv) is a flavivirus transmitted primarily via the bite of infected aedes aegypti mosquitoes. globally, 87 countries and territories have recorded autochthonous mosquito-borne transmission of zikv as at july 2019 and distributed across four of the six who regions. outbreaks of zikv infection peaked in 2016 and declined substantially throughout 2017 and 2018 in the americas region. there is the likely risk for zikv to spread to more countries. there is also the potential for the re-e ... | 2020 | 32056845 |
| understanding risk information seeking and processing during an infectious disease outbreak: the case of zika virus. | this study draws on the planned risk information seeking model (prism) to assess zika virus information seeking and systematic processing, paying particular attention to the relationship between perceived knowledge and knowledge insufficiency. novel risks, such as zika, provide an interesting context for examining whether information-seeking models, such as prism, are able to predict information seeking when available information is limited or scarce. a cross-sectional, online study of men and w ... | 2020 | 32067244 |
| mapping interactions among cell-free expressed zika virus proteins. | the rapid spread of arthropod-borne zika virus poses a serious public health threat that calls for effective ways of controlling and treating viral infection. this in turn necessitates better understanding of the mechanisms of virus assembly and its interaction with the host cells. in order to facilitate such efforts, we developed a new multihost expression vector pmcellfree that allows rapid and multiplexed production of zikv proteins in any in vitro translation system as well as in mammalian c ... | 2020 | 32081002 |
| bpifb3 regulates endoplasmic reticulum morphology to facilitate flavivirus replication. | flaviviruses, including dengue virus (denv) and zika virus (zikv), rely heavily on the availability of endoplasmic reticulum (er) membranes throughout their life cycle, and degradation of er membranes restricts flavivirus replication. accordingly, denv and zikv restrict er turnover by protease-mediated cleavage of reticulophagy regulator 1 (retreg1), also known as fam134b, an autophagy receptor responsible for targeted er sheet degradation. given that the induction of autophagy may play an impor ... | 2020 | 32102874 |
| in vitro zika virus infection of human neural progenitor cells: meta-analysis of rna-seq assays. | the zika virus (zikv) is an emergent arthropod-borne virus (arbovirus) responsible for congenital zika syndrome (czs) and a range of other congenital malformations. evidence shows that zikv infects human neural progenitor cells (hnpcs) in the fetal brain, prompting inflammation and tissue damage/loss. despite recent advances, little is known about the pathways involved in czs pathogenesis. we performed a meta-analysis, gene ontology (go), and pathway analysis of whole transcriptome studies with ... | 2020 | 32079323 |
| the effects of japanese encephalitis virus antibodies on zika virus infection. | recently, zika virus (zikv) has become more widespread, thus attracting global attention. the vaccine against japanese encephalitis virus (jev) is currently used in china, being included in planned immunisation regimes. although zikv and jev are closely related mosquito-borne flaviviruses, and a complex cross-immune response within flaviviruses has been demonstrated, the effect of jev vaccination on zikv infection has not been well described. thus, this study aimed to explore the impact of diffe ... | 2020 | 32078028 |
| a lesson learnt from the emergence of zika virus: what flaviviruses can trigger guillain-barré syndrome? | while zika virus outbreaks raised the concern about rare viral infections in human, attention should also be paid to other guillain-barré syndrome (gbs) inducing viruses. this study aims to search for other flaviviruses likely to be associated with gbs. amino acid (aa) sequence matching analysis was conducted to identify viruses molecularly similar to the zika virus and human gbs-related proteins. a systematic review of clinical literature was performed to summarize the clinical findings of the ... | 2020 | 32077499 |
| a review of the economic evidence of aedes-borne arboviruses and aedes-borne arboviral disease prevention and control strategies. | introduction:aedes-borne arboviruses contributes substantially to the disease and cost burden.areas covered: we performed a systematic review of the economic evidence surrounding aedes-borne arboviruses and strategies to prevent and control these diseases to inform disease control policy decisions and research directions. we searched four databases covering an 18-year period (2000-2018) to identify arboviral disease-related cost of illness studies, cost studies of vector control and prevention s ... | 2020 | 32077343 |
| genomic and epidemiological surveillance of zika virus in the amazon region. | zika virus (zikv) has caused an explosive epidemic linked to severe clinical outcomes in the americas. as of june 2018, 4,929 zikv suspected infections and 46 congenital syndrome cases had been reported in manaus, amazonas, brazil. although manaus is a key demographic hub in the amazon region, little is known about the zikv epidemic there, in terms of both transmission and viral genetic diversity. using portable virus genome sequencing, we generated 59 zikv genomes in manaus. phylogenetic analys ... | 2020 | 32075736 |
| family fortresses in zika congenital syndrome according to betty neuman. | to identify elements that contribute to strengthen the family system of children with zika virus congenital syndrome according to betty neuman's theory. | 2020 | 32074238 |
| biological effects of avicennia marina (forssk.) vierh. extracts on physiological, biochemical, and antimicrobial activities against three challenging mosquito vectors and microbial pathogens. | mosquitoes are principal vector of several vector-borne diseases affecting human beings leading to thousands of deaths per year and responsible for transmitting diseases like malaria, dengue, chikungunya, yellow fever, zika virus, japanese encephalitis, and lymphatic filariasis. in the present study, we evaluated the different solvent extracts of mangrove avicennia marina for their toxicity against larvae of three major mosquito vectors, as well as selected microbial pathogens. the larvicidal mo ... | 2020 | 32072409 |
| alanine substitution inactivates cross-reacting epitopes in dengue virus recombinant envelope proteins. | the expansion of the habitat of mosquitoes belonging to the aedes genus puts nearly half of the world's population at risk of contracting dengue fever, and a significant fraction will develop its serious hemorrhagic complication, which can be fatal if not diagnosed properly and treated in a timely fashion. although several diagnostic methods have been approved for dengue diagnostics, their applicability is limited in rural areas of developing countries by sample preparation costs and methodologi ... | 2020 | 32069839 |