Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| stabilization of a broadly neutralizing anti-chikungunya virus single domain antibody. | a single domain antibody (clone cc3) previously found to neutralize a vaccine strain of the chikungunya virus (prnt50 = 2. 5 ng/ml) was found to be broadly neutralizing. clone cc3 is not only able to neutralize a wild-type (wt) strain of chikungunya virus (chikv), but also neutralizes wt strains of mayaro virus (mayv) and ross river virus (rrv); both arthralgic, old world alphaviruses. interestingly, cc3 also demonstrated a degree of neutralizing activity against the new world alphavirus, venezu ... | 2021 | 33585527 |
| high frequency of mayaro virus igm among febrile patients, central brazil. | mayaro virus (mayv), an aedes mosquito-borne alphavirus, is endemic to brazil and other south america countries. we investigated dengue- and chikungunya-negative febrile patients visiting rural areas near goiânia, goiás, and found a high proportion (55%) of mayv igm. our findings suggest the presence of highly endemic foci of mayv in central brazil. | 2017 | 28518022 |
| oxidative stress in mayaro virus infection. | mayaro virus (mayv) is a neglected tropical arbovirus that causes a febrile syndrome that is sometimes accompanied by incapacitating arthritis/arthralgia. the pathogenesis of mayv has not been completely defined and oxidative stress mediated by an increase in reactive oxygen species (ros) and/or depletion of antioxidant defences has been found to contribute to several aspects of viral disease. to investigate whether mayv induced oxidative stress in host cells, we monitored ros production, oxidat ... | 2017 | 28455127 |
| on the entry of an emerging arbovirus into host cells: mayaro virus takes the highway to the cytoplasm through fusion with early endosomes and caveolae-derived vesicles. | mayaro virus (mayv) is an emergent sylvatic alphavirus in south america, related to sporadic outbreaks of a chikungunya-like human febrile illness accompanied by severe arthralgia. despite its high potential for urban emergence, mayv is still an obscure virus with scarce information about its infection cycle, including the corresponding early events. even for prototypical alphaviruses, the cell entry mechanism still has some rough edges to trim: although clathrin-mediated endocytosis is quoted a ... | 2017 | 28462045 |
| emergence of recombinant mayaro virus strains from the amazon basin. | mayaro virus (mayv), causative agent of mayaro fever, is an arbovirus transmitted by haemagogus mosquitoes. despite recent attention due to the identification of several cases in south and central america and the caribbean, limited information on mayv evolution and epidemiology exists and represents a barrier to prevention of further spread. we present a thorough spatiotemporal evolutionary study of mayv full-genome sequences collected over the last sixty years within south america and haiti, re ... | 2017 | 28821712 |
| will mayaro virus be responsible for the next outbreak of an arthropod-borne virus in brazil? | mayaro virus (mayv) is an alphavirus from the togaviridae family and is transmitted mainly by hemagogus mosquitoes. this virus circulates in high-density tropical forests or rural areas of central and south america causing a disease characterized by high-grade fever, maculopapular skin rash and marked arthralgia that, in some patients, can persist for long periods after infection and may be misinterpreted as chikungunya. although only a few outbreaks involving this virus have been reported, in t ... | 2017 | 28688628 |
| multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of mayaro, oropouche, and oropouche-like viruses. | we describe a sensitive method for simultaneous detection of oropouche and oropouche-like viruses carrying the oropouche s segment, as well as the mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (rt-qpcr). a chimeric plasmid containing both mayaro and oropouche targets was designed and evaluated for the in vitro production of transcribed rna, which could be easily used as a non-infectious external control. to track false-negative results due t ... | 2017 | 28591313 |
| characterization of mitochondrial genome of haemagogus janthinomys (diptera: culicidae). | haemagogus janthinomys is a mosquito of high importance in public health due its involvement on natural wild cycles of two important arboviruses in the brazilian amazon region: yellow fever virus (flaviviridae, flavivirus) and mayaro virus (togaviridae, alphavirus). here, we have sequenced and described all the mitochondrial genes for the hg. janthinomys species. the complete coding sequence is14 937 bp long and includes 37 functional genes, of which 13 codes for proteins, 22 for trna and 2 for ... | 2017 | 26709451 |
| thieno[2,3-b]pyridine derivatives: a new class of antiviral drugs against mayaro virus. | mayaro virus (mayv) is an arthropod-borne virus and a member of the family togaviridae, genus alphavirus. its infection leads to an acute illness accompanied by long-lasting arthralgia. to date, there are no antiviral drugs or vaccines against infection with mayv and resources for the prevention or treatment of other alphaviruses are very limited. mayv has served as a model to study the antiviral potential of several substances on alphavirus replication. in this work we evaluated the antiviral e ... | 2017 | 28213871 |
| mayaro virus: a forest virus primed for a trip to the city? | mayaro virus (mayv) is an emerging arthropod-borne virus (arbovirus). infection by mayv can produce mayaro virus disease (mayvd) which is usually a clinically diagnosed, acute, febrile illness associated with prolonged and painful joint inflammation and swelling. mayvd may be clinically indistinguishable from dengue, chikungunya fever, malaria, rabies, measles or other arboviral diseases. the full spectrum of disease, sequelae, routes of infection, virus shedding and any rarer means of transmiss ... | 2016 | 27989728 |
| mayaro fever in an hiv-infected patient suspected of having chikungunya fever. | arboviruses impose a serious threat to public health services. we report a case of a patient returning from a work trip to the amazon basin with myalgia, arthralgia, fever, and headache. during this travel, the patient visited riverside communities. both dengue and chikungunya fevers were first suspected, tested for, and excluded. mayaro fever was then confirmed by reverse transcription polymerase chain reaction followed by next-generation sequencing and phylogenetic reconstruction. the increase ... | 2016 | 27812665 |
| macrophages as target cells for mayaro virus infection: involvement of reactive oxygen species in the inflammatory response during virus replication. | alphaviruses among the viruses that cause arthritis, consisting in a public health problem worldwide by causing localized outbreaks, as well as large epidemics in humans. interestingly, while the old world alphaviruses are arthritogenic, the new world alphaviruses cause encephalitis. one exception is mayaro virus (mayv), which circulates exclusively in south america but causes arthralgia and is phylogenetically related to the old world alphaviruses. although mayv-induced arthritis in humans is w ... | 2016 | 27627069 |
| a case of mayaro virus infection imported from french guiana. | emergence of arboviruses is a rising problem in several areas in the world. here we report a case of mayaro virus infection that was diagnosed in a french citizen presenting a dengue-like syndrome with prolonged arthralgia following a travel in french guiana. diagnosis was based on serological testing, a newly developed specific rt-pcr and sequencing. the real incidence of this viral infection among travelers is poorly known but this case is the first reported in a european area where aedes albo ... | 2016 | 26921736 |
| chikungunya: epidemiology. | chikungunya virus is a mosquito-borne alphavirus that causes fever and debilitating joint pains in humans. joint pains may last months or years. it is vectored primarily by the tropical and sub-tropical mosquito, aedes aegypti, but is also found to be transmitted by aedes albopictus, a mosquito species that can also be found in more temperate climates. in recent years, the virus has risen from relative obscurity to become a global public health menace affecting millions of persons throughout the ... | 2016 | 26918158 |
| global research trends of middle east respiratory syndrome coronavirus: a bibliometric analysis. | middle east respiratory syndrome coronavirus (mers-cov) is a virus that causes severe viral pneumonia in humans, known to have a high mortality rate and a similarity in clinical symptoms with severe acute respiratory syndrome coronavirus. it was first isolated in kingdom of saudi arabia (ksa) in 2012 and after that, mers-cov exhibited outbreaks in several regions of the world. this study aimed to assess the characteristics of publications involving mers-cov at global level by using a bibliometri ... | 2016 | 27267256 |
| an extract from taxodium distichum targets hemagglutinin- and neuraminidase-related activities of influenza virus in vitro. | influenza virus remains an emerging virus and causes pandemics with high levels of fatality. after screening different plant extracts with potential anti-influenza activity, a water extract of taxodium distichum stems (tdswex) showed excellent activity against influenza viruses. the ec50 of tdswex was 0.051 ± 0.024 mg/ml against influenza virus a/wsn/33. tdswex had excellent antiviral efficacy against various strains of human influenza a and b viruses, particularly oseltamivir-resistant clinical ... | 2016 | 27796330 |
| dna microarray platform for detection and surveillance of viruses transmitted by small mammals and arthropods. | viruses transmitted by small mammals and arthropods serve as global threats to humans. most emergent and re-emergent viral agents are transmitted by these groups; therefore, the development of high-throughput screening methods for the detection and surveillance of such viruses is of great interest. in this study, we describe a dna microarray platform that can be used for screening all viruses transmitted by small mammals and arthropods (smaviruschip) with nucleotide sequences that have been depo ... | 2016 | 27654806 |
| chikungunya virus transmission between aedes albopictus and laboratory mice. | chikungunya virus (chikv) is a mosquito-borne alphavirus associated with epidemics of acute and chronic arthritic disease in humans. aedes albopictus has emerged as an important new natural vector for chikv transmission; however, mouse models for studying transmission have not been developed. | 2016 | 27760560 |
| pyruvate dehydrogenase kinase regulates hepatitis c virus replication. | during replication, hepatitis c virus (hcv) utilizes macromolecules produced by its host cell. this process requires host cellular metabolic reprogramming to favor elevated levels of aerobic glycolysis. therefore, we evaluated whether pyruvate dehydrogenase kinase (pdk), a mitochondrial enzyme that promotes aerobic glycolysis, can regulate hcv replication. levels of c-myc, hypoxia-inducible factor-1α (hif-1α), pdk1, pdk3, glucokinase, and serine biosynthetic enzymes were compared between hcv-inf ... | 2016 | 27471054 |
| searching for putative avian malaria vectors in a seasonally dry tropical forest in brazil. | haemosporidian parasites of the genera plasmodium and haemoproteus can have detrimental effects on individual birds and populations. despite recent investigations into the distribution and richness of these parasites and their vertebrate hosts, little is known about their dipteran vectors. the neotropics has the highest diversity of mosquitoes in the world, but few studies have tried to identify vectors in this area, hampering the understanding of the ecology of avian malaria in the highly diver ... | 2016 | 27852326 |
| serosurvey of selected arboviral pathogens in free-ranging, two-toed sloths (choloepus hoffmanni) and three-toed sloths (bradypus variegatus) in costa rica, 2005-07. | we screened for antibodies to 16 arboviruses in four populations of free-ranging sloths in costa rica. blood samples were taken from 16 hoffman's two-toed sloths (htss; choloepus hoffmanni ) and 26 brown-throated sloths (btss; bradypus variegatus ) over a 3-yr period. we used serologic assays to detect antibodies against 10 arboviruses previously described in sloths (st. louis encephalitis [slev], changuinola, venezuelan equine encephalitis, ilheus [ilhv], oropouche, mayaro, utinga, murutucu, pu ... | 2016 | 27479900 |
| rickettsial disease in the peruvian amazon basin. | using a large, passive, clinic-based surveillance program in iquitos, peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. blood specimens from humans and animals were assayed for spotted fever group rickettsiae (sfgr) and typhus group rickettsiae (tgr) by elisa and/or pcr; ectoparasites were screened by pcr. logistic regression was used ... | 2016 | 27416029 |
| dengue research: a bibliometric analysis of worldwide and arab publications during 1872-2015. | dengue is an important emerging and re-emerging arboviral infection globally as a rapidly growing and widespread public health problem, with transmission occurring in more than 128 countries in asia, americas, southeast africa, western pacific, and eastern mediterranean regions. therefore, the aim of this study was to characterize and quantify the scientific output of dengue research in arab countries relative to that worldwide by using a bibliometric analysis. | 2016 | 27154247 |
| the viral capping enzyme nsp1: a novel target for the inhibition of chikungunya virus infection. | the chikungunya virus (chikv) has become a substantial global health threat due to its massive re-emergence, the considerable disease burden and the lack of vaccines or therapeutics. we discovered a novel class of small molecules ([1,2,3]triazolo[4,5-d]pyrimidin-7(6h)-ones) with potent in vitro activity against chikv isolates from different geographical regions. drug-resistant variants were selected and these carried a p34s substitution in non-structural protein 1 (nsp1), the main enzyme involve ... | 2016 | 27545976 |
| celebration of 60th anniversary of brazilian society for microbiology. | 2016 | 27865633 | |
| mosquito-transmitted viruses - the great brazilian challenge. | arboviruses pose a serious threat to public health worldwide, overloading the healthcare system and causing economic losses. these viruses form a very diverse group, and in brazil, arboviruses belonging to the families flaviviridae and togaviridae are predominant. unfortunately, the number of arboviruses increases in proportion with factors such as deforestation, poor sanitation, climate changes, and introduction of new viruses like chikungunya virus and zika virus. in brazil, dengue is endemic, ... | 2016 | 27818091 |
| a field-deployable reverse transcription recombinase polymerase amplification assay for rapid detection of the chikungunya virus. | chikungunya virus (chikv) is a mosquito-borne virus currently transmitted in about 60 countries. chikv causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. detection of the infection is mostly done using rt-rcr or elisa, which are not suitable for point-of-care diagnosis. | 2016 | 27685649 |
| hierarchical cluster analysis of three-dimensional reconstructions of unbiased sampled microglia shows not continuous morphological changes from stage 1 to 2 after multiple dengue infections in callithrix penicillata. | it is known that microglial morphology and function are related, but few studies have explored the subtleties of microglial morphological changes in response to specific pathogens. in the present report we quantitated microglia morphological changes in a monkey model of dengue disease with virus cns invasion. to mimic multiple infections that usually occur in endemic areas, where higher dengue infection incidence and abundant mosquito vectors carrying different serotypes coexist, subjects receiv ... | 2016 | 27047345 |
| an rna trapping mechanism in alphavirus mrna promotes ribosome stalling and translation initiation. | during translation initiation, eukaryotic initiation factor 2 (eif2) delivers the met-trna to the 40s ribosomal subunit to locate the initiation codon (augi) of mrna during the scanning process. stress-induced eif2 phosphorylation leads to a general blockade of translation initiation and represents a key antiviral pathway in mammals. however, some viral mrnas can initiate translation in the presence of phosphorylated eif2 via stable rna stem-loop structures (dlp; downstream loop) located in thei ... | 2016 | 26984530 |
| mrna capping by venezuelan equine encephalitis virus nsp1: functional characterization and implications for antiviral research. | alphaviruses are known to possess a unique viral mrna capping mechanism involving the viral nonstructural protein nsp1. this enzyme harbors methyltransferase (mtase) and nsp1 guanylylation (gt) activities catalyzing the transfer of the methyl group from s-adenosylmethionine (adomet) to the n7 position of a gtp molecule followed by the formation of an m(7)gmp-nsp1 adduct. subsequent transfer of m(7)gmp onto the 5' end of the viral mrna has not been demonstrated in vitro yet. here we report the bi ... | 2015 | 26041283 |
| bovine lactoferrin inhibits toscana virus infection by binding to heparan sulphate. | toscana virus is an emerging sandfly-borne bunyavirus in mediterranean europe responsible for neurological diseases in humans. it accounts for about 80% of paediatric meningitis cases during the summer. despite the important impact of toscana virus infection-associated disease on human health, currently approved vaccines or effective antiviral treatments are not available. in this research, we have analyzed the effect of bovine lactoferrin, a bi-globular iron-binding glycoprotein with potent ant ... | 2015 | 25643293 |
| genetic diversity of venezuelan alphaviruses and circulation of a venezuelan equine encephalitis virus subtype iab strain during an interepizootic period. | several species of alphaviruses have been previously described in the americas, some of which are associated with encephalitis and others are associated with arthralgia. venezuelan equine encephalitis virus (veev) and eastern equine encephalitis virus (eeev) are endemic to venezuela, with the former being responsible for major outbreaks of severe and often fatal disease in animals and humans. the aim of this study was to analyze the genetic diversity of venezuelan alphaviruses isolated during tw ... | 2015 | 25940191 |
| syndromic approach to arboviral diagnostics for global travelers as a basis for infectious disease surveillance. | arboviruses have overlapping geographical distributions and can cause symptoms that coincide with more common infections. therefore, arbovirus infections are often neglected by travel diagnostics. here, we assessed the potential of syndrome-based approaches for diagnosis and surveillance of neglected arboviral diseases in returning travelers. | 2015 | 26372010 |
| long-term arthralgia after mayaro virus infection correlates with sustained pro-inflammatory cytokine response. | mayaro virus (mayv), an alphavirus similar to chikungunya virus (chikv), causes an acute debilitating disease which results in the development of long-term arthralgia in more than 50% of infected individuals. currently, the immune response and its role in the development of mayv-induced persistent arthralgia remain unknown. in this study, we evaluated the immune response of individuals with confirmed mayv infection in a one-year longitudinal study carried out in loreto, peru. we report that mayv ... | 2015 | 26496497 |
| global emergence of alphaviruses that cause arthritis in humans. | arthropod-borne viruses (arboviruses) may cause severe emerging and re-emerging infectious diseases, which pose a significant threat to human and animal health in the world today. these infectious diseases range from mild febrile illnesses, arthritis, and encephalitis to haemorrhagic fevers. it is postulated that certain environmental factors, vector competence, and host susceptibility have a major impact on the ecology of arboviral diseases. presently, there is a great interest in the emergence ... | 2015 | 26689654 |
| broadly neutralizing alphavirus antibodies bind an epitope on e2 and inhibit entry and egress. | we screened a panel of mouse and human monoclonal antibodies (mabs) against chikungunya virus and identified several with inhibitory activity against multiple alphaviruses. passive transfer of broadly neutralizing mabs protected mice against infection by chikungunya, mayaro, and o'nyong'nyong alphaviruses. using alanine-scanning mutagenesis, loss-of-function recombinant proteins and viruses, and multiple functional assays, we determined that broadly neutralizing mabs block multiple steps in the ... | 2015 | 26553503 |
| extended preclinical safety, efficacy and stability testing of a live-attenuated chikungunya vaccine candidate. | we recently described a new, live-attenuated vaccine candidate for chikungunya (chik) fever, chikv/ires. this vaccine was shown to be well attenuated, immunogenic and efficacious in protecting against chik virus (chikv) challenge of mice and nonhuman primates. to further evaluate its preclinical safety, we compared chikv/ires distribution and viral loads in interferon-α/β receptor-incompetent a129 mice to another chik vaccine candidate, 181/clone25, which proved highly immunogenic but mildly rea ... | 2015 | 26340754 |
| divergent cerebrospinal fluid cytokine network induced by non-viral and different viral infections on the central nervous system. | meningoencephalitis is one of the most common disorders of the central nervous system (cns) worldwide. viral meningoencephalitis differs from bacterial meningitis in several aspects. in some developing countries, bacterial meningitis has appropriate clinical management and chemotherapy is available. virus-associated and virus not detected meningoencephalitis are treatable, however, they may cause death in a few cases. the knowledge of how mediators of inflammation can induce disease would contri ... | 2015 | 26286516 |
| utilization of an eilat virus-based chimera for serological detection of chikungunya infection. | in december of 2013, chikungunya virus (chikv), an alphavirus in the family togaviridae, was introduced to the island of saint martin in the caribbean, resulting in the first autochthonous cases reported in the americas. as of january 2015, local and imported chikv has been reported in 50 american countries with over 1.1 million suspected cases. chikv causes a severe arthralgic disease for which there are no approved vaccines or therapeutics. furthermore, the lack of a commercially available, se ... | 2015 | 26492074 |
| proteomics profiling of chikungunya-infected aedes albopictus c6/36 cells reveal important mosquito cell factors in virus replication. | chikungunya virus (chikv) is the only causative agent of chikv fever with persistent arthralgia, and in some cases may lead to neurological complications which can be highly fatal, therefore it poses severe health issues in many parts of the world. chikv transmission can be mediated via the aedes albopictus mosquito; however, very little is currently known about the involvement of mosquito cellular factors during chikv-infection within the mosquito cells. unravelling the neglected aspects of mos ... | 2015 | 25738640 |
| neutralising antibodies for mayaro virus in pantanal, brazil. | the pantanal hosts diverse wildlife species and therefore is a hotspot for arbovirus studies in south america. a serosurvey for mayaro virus (mayv), eastern (eeev), western (weev) and venezuelan (veev) equine encephalitis viruses was conducted with 237 sheep, 87 free-ranging caimans and 748 equids, including 37 collected from a ranch where a neurologic disorder outbreak had been recently reported. sera were tested for specific viral antibodies using plaque-reduction neutralisation test. from a t ... | 2015 | 25742272 |
| emergence and potential for spread of chikungunya virus in brazil. | in december 2013, an outbreak of chikungunya virus (chikv) caused by the asian genotype was notified in the caribbean. the outbreak has since spread to 38 regions in the americas. by september 2014, the first autochthonous chikv infections were confirmed in oiapoque, north brazil, and in feira de santana, northeast brazil. | 2015 | 25976325 |
| new records of mosquitoes (diptera: culicidae) from bolívar state in south eastern venezuela, with 27 new species for the state and 5 of them new in the country. | this is the first part of a series of studies related to mosquito ecological and biogeographic aspects. a total of 69 mosquito species (diptera: culicidae) was collected in 16 localities sampled in the gran sabana municipality, canaima national park, and venezuela. twenty-seven mosquito species are recorded for the first time from bolívar state, venezuela. five of them species are reported for the first time in venezuela: anopheles malefactor dyar and knab (1907); chagasia bonneae root (1927); c ... | 2015 | 25853113 |
| dengue virus ns1 protein modulates cellular energy metabolism by increasing glyceraldehyde-3-phosphate dehydrogenase activity. | dengue is one of the main public health concerns worldwide. recent estimates indicate that over 390 million people are infected annually with the dengue virus (denv), resulting in thousands of deaths. among the denv nonstructural proteins, the ns1 protein is the only one whose function during replication is still unknown. ns1 is a 46- to 55-kda glycoprotein commonly found as both a membrane-associated homodimer and a soluble hexameric barrel-shaped lipoprotein. despite its role in the pathogenic ... | 2015 | 26378175 |
| complete genome sequence of mayaro virus (togaviridae, alphavirus) strain bear 20290 from brazil. | we report here the complete genome sequence of mayaro virus strain bear 20290 isolated from haemagogus mosquitoes in 1960. the sequence presented here includes all nonstructural and structural proteins and the 5'- and 3'-untranslated (utr) regions. | 2015 | 26679574 |
| complete genome sequence of mayaro virus imported from the amazon basin to são paulo state, brazil. | mayaro (mayv) is a neglected arbovirus from the tropical americas. here, we report the complete genome of an mayv isolate from a patient returning from the amazon basin and complaining of arthralgia, high fever, and headache, who was attended at an emergency service of são josé do rio preto, são paulo state, brazil. | 2015 | 26607884 |
| emerging alphaviruses in the americas: chikungunya and mayaro. | chikungunya virus (chikv) and mayaro virus (mayv) are emergent arthropod-borne viruses that produce outbreaks of acute febrile illness with arthropathy. despite their different continental origins, chikv and mayv are closely related and are components of the semliki forest complex of the alphavirus (togaviridae). mayv and, more recently, chikv, which are both transmitted by aedes mosquitoes, have resulted in severe public health problems in the americas, including brazil. in this review, we pres ... | 2015 | 25626645 |
| detection of mayaro virus infections during a dengue outbreak in mato grosso, brazil. | arboviruses are common agents of human febrile illness worldwide. in dengue-endemic areas illness due to other arboviruses have been misdiagnosed as dengue based only on clinical-epidemiological data. in this study we investigated the presence of brazilian arboviruses in sera of 200 patients presenting acute febrile illness, during a dengue outbreak in sinop, mt, brazil. the results showed that 38 samples were positive to dengue virus (denv) type 1, two samples to denv type 4, and six to mayaro ... | 2015 | 25817238 |
| peptide motif analysis predicts alphaviruses as triggers for rheumatoid arthritis. | rheumatoid arthritis (ra) develops in response to both genetic and environmental factors. the strongest genetic determinant is hla-dr, where polymorphisms within the p4 and p6 binding pockets confer elevated risk. however, low disease concordance across monozygotic twin pairs underscores the importance of an environmental factor, probably infectious. the goal of this investigation was to predict the microorganism most likely to interact with hla-dr to trigger ra under the molecular mimicry hypot ... | 2015 | 26476978 |
| neutralising antibodies for mayaro virus in pantanal, brazil. | the pantanal hosts diverse wildlife species and therefore is a hotspot for arbovirus studies in south america. a serosurvey for mayaro virus (mayv), eastern (eeev), western (weev) and venezuelan (veev) equine encephalitis viruses was conducted with 237 sheep, 87 free-ranging caimans and 748 equids, including 37 collected from a ranch where a neurologic disorder outbreak had been recently reported. sera were tested for specific viral antibodies using plaque-reduction neutralisation test. from a t ... | 2015 | 25650766 |
| nmr study of non-structural proteins--part i: (1)h, (13)c, (15)n backbone and side-chain resonance assignment of macro domain from mayaro virus (mayv). | macro domains are adp-ribose-binding modules present in all eukaryotic organisms, bacteria and archaea. they are also found in non-structural proteins of several positive strand rna viruses such as alphaviruses. here, we report the high yield expression and preliminary structural analysis through solution nmr spectroscopy of the macro domain from new world mayaro alphavirus. the recombinant protein was well-folded and in a monomeric state. an almost complete sequence-specific assignment of its ( ... | 2015 | 25217003 |
| persisting arthralgia due to mayaro virus infection in a traveler from brazil: is there a risk for attendants to the 2014 fifa world cup? | the 2014 fifa world cup and the 2016 olympic games will attract large groups of visitors to brazil. these visitors will be at risk for different arboviral infections, some of which not well known outside endemic areas. we report a case of a 52-year-old dutch woman who presented with persistent arthralgia due to a mayaro virus (mayv) infection which she contracted in the amazon basin in brazil. mayv is a mosquito-borne alphavirus which primarily circulates in humid tropical forests of south ameri ... | 2014 | 24856445 |
| hepatitis a and e seroprevalence and associated risk factors: a community-based cross-sectional survey in rural amazonia. | hepatitis a virus (hav) and hepatitis e virus (hev) are both transmitted by the faecal-oral route, and represent common causes of acute hepatitis in developing countries. the endemicity of hav infection has shifted from high to moderate in brazil. human cases of hev infection seem to be rare, although the virus has been detected in swine livestock and effluents of slaughterhouses. this study was to determine the epidemiology of hepatitis a and e in one of the largest agricultural settlements in ... | 2014 | 25149658 |
| molecular detection of mayaro virus during a dengue outbreak in the state of mato grosso, central-west brazil. | mayaro virus (mayv) is frequently reported in pan-amazonia. the aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of mato grosso, brazil. serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptase polymerase chain reaction for 11 flaviviruses and five alphaviruses, to nucleotide sequencing and to viral isolation. mayv was detected in 15 (2.5%) of 604 patients. twelve were co-infected with den ... | 2014 | 25141284 |
| isolation and characterization of mayaro virus from a human in acre, brazil. | mayaro virus (mayv) is widely distributed throughout south america and is the etiologic agent of mayaro fever, an acute febrile illness often presenting with arthralgic manifestations. the true incidence of mayv infection is likely grossly underestimated because the symptomatic presentation is very similar to that of dengue fever and other acute febrile tropical diseases. we report the complete genome sequence of a mayv isolate detected from an acrelândia patient presenting with fever, chills, a ... | 2014 | 25510721 |
| inhibition of mayaro virus infection by bovine lactoferrin. | mayaro virus (mayv) is an arbovirus linked to several sporadic outbreaks of a highly debilitating febrile illness in many regions of south america. mayv is on the verge of urbanization from the amazon region and no effective antiviral intervention is available against human infections. our aim was to investigate whether bovine lactoferrin (blf), an iron-binding glycoprotein, could hinder mayv infection. we show that blf promotes a strong inhibition of virus infection with no cytotoxic effects. m ... | 2014 | 24606707 |
| detection of arboviruses of public health interest in free-living new world primates (sapajus spp.; alouatta caraya) captured in mato grosso do sul, brazil. | a sero-epidemiological survey was undertaken to detect the circulation of arboviruses in free-living non-human primates. | 2014 | 24474008 |
| quercetin and quercetin 3-o-glycosides from bauhinia longifolia (bong.) steud. show anti-mayaro virus activity. | the arthropod-borne mayaro virus (mayv) causes 'mayaro fever', a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical south america. recently, mayv has attracted attention due to its likely urbanization. currently, there are no licensed drugs against most mosquito-transmitted viruses. here, we investigated the in vitro anti-mayv activity of the flavonoids quercetin and its derivatives from the brazilian shrub bauhinia longifolia (b ... | 2014 | 24678592 |
| anti-mayaro virus activity of cassia australis extracts (fabaceae, leguminosae). | the arthropod-borne mayaro virus (mayv) causes 'mayaro fever', a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical south america. studies showed that the virus could also be transmitted by the mosquito aedes aegypti. recently, mayv has attracted attention due to its likely urbanization. to date, there are no drugs that can treat this illness. | 2014 | 25428163 |
| phospholipase a2 isolated from the venom of crotalus durissus terrificus inactivates dengue virus and other enveloped viruses by disrupting the viral envelope. | the flaviviridae family includes several virus pathogens associated with human diseases worldwide. within this family, dengue virus is the most serious threat to public health, especially in tropical and sub-tropical regions of the world. currently, there are no vaccines or specific antiviral drugs against dengue virus or against most of the viruses of this family. therefore, the development of vaccines and the discovery of therapeutic compounds against the medically most important flaviviruses ... | 2014 | 25383618 |
| seroprevalence of alphavirus antibodies in a cross-sectional study in southwestern tanzania suggests endemic circulation of chikungunya. | to date, alphavirus infections and their most prominent member, chikungunya fever, a viral disease which first became apparent in tanzania in 1953, have been very little investigated in regions without epidemic occurrence. few data exist on burden of disease and socio-economic and environmental covariates disposing to infection. | 2014 | 25079964 |
| occurrence and correlates of symptom persistence following acute dengue fever in peru. | dengue virus (denv) infection causes an acute febrile illness generally considered to result in either complete recovery or death. some reviews describe persistent symptoms after the febrile phase, although empirical data supporting this phenomenon is scarce. we evaluated symptom persistence in acute febrile denv-infected and denv-negative (controls) individuals from peru. self-reported solicited symptoms were evaluated at an acute and a follow-up visit, occurring 10-60 days after symptom onset. ... | 2014 | 24470564 |
| clinical and virological descriptive study in the 2011 outbreak of dengue in the amazonas, brazil. | dengue is a vector-borne disease in the tropical and subtropical region of the world and is transmitted by the mosquito aedes aegypti. in the state of amazonas, brazil during the 2011 outbreak of dengue all the four dengue virus (denv) serotypes circulating simultaneously were observed. the aim of the study was to describe the clinical epidemiology of dengue in manaus, the capital city of the state of the amazonas, where all the four denv serotypes were co-circulating simultaneously. | 2014 | 24978469 |
| development of an algorithm for production of inactivated arbovirus antigens in cell culture. | arboviruses are medically important pathogens that cause human disease ranging from a mild fever to encephalitis. laboratory diagnosis is essential to differentiate arbovirus infections from other pathogens with similar clinical manifestations. the arboviral diseases branch (adb) reference laboratory at the cdc division of vector-borne diseases (dvbd) produces reference antigens used in serological assays such as the virus-specific immunoglobulin m antibody-capture enzyme-linked immunosorbent as ... | 2014 | 25102428 |
| a novel live-attenuated vaccine candidate for mayaro fever. | mayaro virus (mayv) is an emerging, mosquito-borne alphavirus that causes a dengue-like illness in many regions of south america, and which has the potential to urbanize. because no specific treatment or vaccine is available for mayv infection, we capitalized on an ires-based approach to develop a live-attenuated mayv vaccine candidate. testing in infant, immunocompetent as well as interferon receptor-deficient mice demonstrated a high degree of attenuation, strong induction of neutralizing anti ... | 2014 | 25101995 |
| diagnosis of arboviruses using indirect sandwich igg elisa in horses from the brazilian amazon. | the amazon as a whole is the largest reservoir of arboviruses worldwide, while the brazilian amazon hosts the largest variety of arboviruses isolated to date. in this study, the results of an indirect sandwich igg elisa, standardized for 19 arbovirustypes circulating among horses in brazilian amazon, were compared to results of the hemagglutination inhibition test. a screening test assessed the conditional probability distribution and a pearson linear correlation test determined the correlation ... | 2014 | 25013410 |
| ccr2 deficiency promotes exacerbated chronic erosive neutrophil-dominated chikungunya virus arthritis. | chikungunya virus (chikv) is a member of a globally distributed group of arthritogenic alphaviruses that cause weeks to months of debilitating polyarthritis/arthralgia, which is often poorly managed with current treatments. arthritic disease is usually characterized by high levels of the chemokine ccl2 and a prodigious monocyte/macrophage infiltrate. several inhibitors of ccl2 and its receptor ccr2 are in development and may find application for treatment of certain inflammatory conditions, incl ... | 2014 | 24696480 |
| closing the gap between viral and noninfectious arthritis. | 2014 | 24733902 | |
| attenuating mutations in nsp1 reveal tissue-specific mechanisms for control of ross river virus infection. | ross river virus (rrv) is one of a group of mosquito-transmitted alphaviruses that cause debilitating, and often chronic, musculoskeletal disease in humans. previously, we reported that replacement of the nonstructural protein 1 (nsp1) gene of the mouse-virulent rrv strain t48 with that from the mouse-avirulent strain dc5692 generated a virus that was attenuated in a mouse model of disease. here we find that the six nsp1 nonsynonymous nucleotide differences between strains t48 and dc5692 are det ... | 2014 | 24429363 |
| chikungunya virus-vector interactions. | chikungunya virus (chikv) is a mosquito-borne alphavirus that causes chikungunya fever, a severe, debilitating disease that often produces chronic arthralgia. since 2004, chikv has emerged in africa, indian ocean islands, asia, europe, and the americas, causing millions of human infections. central to understanding chikv emergence is knowledge of the natural ecology of transmission and vector infection dynamics. this review presents current understanding of chikv infection dynamics in mosquito v ... | 2014 | 25421891 |
| multiple immune factors are involved in controlling acute and chronic chikungunya virus infection. | the recent epidemic of the arthritogenic alphavirus, chikungunya virus (chikv) has prompted a quest to understand the correlates of protection against virus and disease in order to inform development of new interventions. herein we highlight the propensity of chikv infections to persist long term, both as persistent, steady-state, viraemias in multiple b cell deficient mouse strains, and as persistent rna (including negative-strand rna) in wild-type mice. the knockout mouse studies provided evid ... | 2014 | 25474568 |
| evolutionary genetics and vector adaptation of recombinant viruses of the western equine encephalitis antigenic complex provides new insights into alphavirus diversity and host switching. | western equine encephalitis virus (weev), highlands j virus (hjv), and fort morgan virus (fmv) are the sole representatives of the wee antigenic complex of the genus alphavirus, family togaviridae, that are endemic to north america. all three viruses have their ancestry in a recombination event involving eastern equine encephalitis virus (eeev) and a sindbis (sin)-like virus that gave rise to a chimeric alphavirus that subsequently diversified into the present-day weev, hjv, and fmv. here, we pr ... | 2014 | 25463613 |
| in- silico exploration of thirty alphavirus genomes for analysis of the simple sequence repeats. | the compilation of simple sequence repeats (ssrs) in viruses and its analysis with reference to incidence, distribution and variation would be instrumental in understanding the functional and evolutionary aspects of repeat sequences. present study encompasses the analysis of ssrs across 30 species of alphaviruses. the full length genome sequences, assessed from ncbi were used for extraction and analysis of repeat sequences using imex software. the repeats of different motif sizes (mono- to penta ... | 2014 | 25606453 |
| chikungunya fever in canada: fever and polyarthritis in a returned traveller. | 2014 | 24566646 | |
| dendritic cell immunoreceptor regulates chikungunya virus pathogenesis in mice. | chikungunya virus (chikv) is a mosquito-borne alphavirus responsible for recent epidemic outbreaks of debilitating disease in humans. alphaviruses are known to interact with members of the c-type lectin receptor family of pattern recognition proteins, and given that the dendritic cell immunoreceptor (dcir) is known to act as a negative regulator of the host inflammatory response and has previously been associated with rheumatoid arthritis, we evaluated dcir's role in response to chikv infection. ... | 2013 | 23487448 |
| a tyrosine-to-histidine switch at position 18 of the ross river virus e2 glycoprotein is a determinant of virus fitness in disparate hosts. | arthritogenic alphaviruses are human pathogens maintained in nature through alternating replication in vertebrates and mosquitoes. using chimeric viruses, we previously reported that replacement of the pe2 coding region of the t48 strain of ross river virus (rrv-t48) with that from the attenuated dc5692 strain, which differ by 7 amino acids, resulted in an attenuated disease phenotype in a mouse model of rrv-induced rheumatic disease. here, we demonstrate that introduction of one of these amino ... | 2013 | 23514884 |
| prolonged polyarthralgia in a german traveller with mayaro virus infection without inflammatory correlates. | mayaro virus is endemic in south america and sporadic outbreaks have been described. it causes a dengue-like febrile illness accompanied by severe and long-lasting polyarthralgias. outside endemic regions, however, the disease is not well known and can be misdiagnosed as dengue. international travellers are at risk to acquire mayaro virus and due to increased worldwide travel infectious disease specialists need to be aware of such rare clinical entities. | 2013 | 23927600 |
| full inactivation of human influenza virus by high hydrostatic pressure preserves virus structure and membrane fusion while conferring protection to mice against infection. | whole inactivated vaccines (wivs) possess greater immunogenicity than split or subunit vaccines, and recent studies have demonstrated that wivs with preserved fusogenic activity are more protective than non-fusogenic wivs. in this work, we describe the inactivation of human influenza virus x-31 by high hydrostatic pressure (hhp) and analyze the effects on the structure by spectroscopic measurements, light scattering, and electron microscopy. we also investigated the effects of hhp on the glycopr ... | 2013 | 24282553 |
| performance of the tourniquet test for diagnosing dengue in peru. | the tourniquet test (tt) is a physical examination maneuver often performed on patients suspected of having dengue. it has been incorporated into dengue diagnostic guidelines and is used in clinical studies. however, little is known about tt performance characteristics in different patient types or epidemiologic conditions. in the dengue-endemic city of iquitos, peru, we performed tts and dengue laboratory assays on 13,548 persons with febrile disease, recruited through either active (n = 1,095) ... | 2013 | 23716410 |
| identification of novel compounds inhibiting chikungunya virus-induced cell death by high throughput screening of a kinase inhibitor library. | chikungunya virus (chikv) is a mosquito-borne arthrogenic alphavirus that causes acute febrile illness in humans accompanied by joint pains and in many cases, persistent arthralgia lasting weeks to years. the re-emergence of chikv has resulted in numerous outbreaks in the eastern hemisphere, and threatens to expand in the foreseeable future. unfortunately, no effective treatment is currently available. the present study reports the use of resazurin in a cell-based high-throughput assay, and an i ... | 2013 | 24205414 |
| a genome-wide analysis of rna pseudoknots that stimulate efficient -1 ribosomal frameshifting or readthrough in animal viruses. | programmed -1 ribosomal frameshifting (prf) and stop codon readthrough are two translational recoding mechanisms utilized by some rna viruses to express their structural and enzymatic proteins at a defined ratio. efficient recoding usually requires an rna pseudoknot located several nucleotides downstream from the recoding site. to assess the strategic importance of the recoding pseudoknots, we have carried out a large scale genome-wide analysis in which we used an in-house developed program to d ... | 2013 | 24298557 |
| a fully attenuated recombinant salmonid alphavirus becomes pathogenic through a single amino acid change in the e2 glycoprotein. | a recombinant sleeping disease virus (rsdv) was previously shown to be totally attenuated and provide long-term protection in trout (c. moriette, m. leberre, a. lamoureux, t. l. lai, m. brémont, j. virol. 80:4088-4098, 2006). sequence comparison of the rsdv to wild-type genomes exhibited a number of nucleotide changes. in the current study, we demonstrate that the virulent phenotype of sdv was essentially associated with two amino acid changes, v8a and m136t, in the e2 glycoprotein, with the v8a ... | 2013 | 23449806 |
| molecular mechanisms involved in the pathogenesis of alphavirus-induced arthritis. | arthritogenic alphaviruses, including ross river virus (rrv), chikungunya virus (chikv), sindbis virus (sinv), mayaro virus (mayv), o'nyong-nyong virus (onnv), and barmah forest virus (bfv), cause incapacitating and long lasting articular disease/myalgia. outbreaks of viral arthritis and the global distribution of these diseases point to the emergence of arthritogenic alphaviruses as an important public health problem. this review discusses the molecular mechanisms involved in alphavirus-induced ... | 2013 | 24069610 |
| differential unfolded protein response during chikungunya and sindbis virus infection: chikv nsp4 suppresses eif2α phosphorylation. | chikungunya (chikv) and sindbis (sinv) are arboviruses belonging to the alphavirus genus within the togaviridae family. they cause frequent epidemics of febrile illness and long-term arthralgic sequelae that affect millions of people each year. both viruses replicate prodigiously in infected patients and in vitro in mammalian cells, suggesting some level of control over the host cellular translational machinery that senses and appropriately directs the cell's fate through the unfolded protein re ... | 2013 | 23356742 |
| global mapping of infectious disease. | the primary aim of this review was to evaluate the state of knowledge of the geographical distribution of all infectious diseases of clinical significance to humans. a systematic review was conducted to enumerate cartographic progress, with respect to the data available for mapping and the methods currently applied. the results helped define the minimum information requirements for mapping infectious disease occurrence, and a quantitative framework for assessing the mapping opportunities for all ... | 2013 | 23382431 |
| interleukin-27 treated human macrophages induce the expression of novel micrornas which may mediate anti-viral properties. | interleukin-27 (il-27) is a pleiotropic cytokine which plays important and diverse roles in the immune system. we have previously demonstrated that il-27 induces potent anti-viral effects against hiv-1, hiv-2, siv, hsv-2, kshv and influenza viruses in macrophages. this induction occurred in an interferon (ifn) independent manner and involved down regulation of sptbn1. micrornas (mirnas) are critical regulators of mrna translation and turnover. there have been reports that some mirnas inhibit vir ... | 2013 | 23535375 |
| assessment of plaque assay methods for alphaviruses. | viruses from the alphavirus genus are responsible for numerous arboviral diseases impacting human health throughout the world. confirmation of acute alphavirus infection is based on viral isolation, identification of viral rna, or a fourfold or greater increase in antibody titers between acute and convalescent samples. in convalescence, the specificity of antibodies to an alphavirus may be confirmed by plaque reduction neutralization test. to identify the best method for alphavirus and neutraliz ... | 2013 | 23085307 |
| arthritogenic alphaviruses--an overview. | mosquito-transmitted alphaviruses causing human rheumatic disease are globally distributed and include chikungunya virus, ross river virus, barmah forest virus, sindbis virus, o'nyong-nyong virus and mayaro virus. these viruses cause endemic disease and, occasionally, large epidemics; for instance, the 2004-2011 chikungunya epidemic resulted in 1.4-6.5 million cases, with imported cases reported in nearly 40 countries. the disease is usually self-limiting and characterized by acute and chronic s ... | 2012 | 22565316 |
| [mayaro: a re-emerging arbovirus in venezuela and latin america]. | mayaro virus produces nonspecific, sublethal disease symptoms, often confused with dengue, but with symptoms of arthalgias that can cause incapacitating disability. outbreaks have been localized and sporadic in the pan-amazonia forest since its first isolation in 1954 (trinidad and tobago). the literature available is scarce, diverse and dispersed. mayaro virus is an alphavirus, phylogenetically related to the semliki forest antigenic complex. una and mayaro viruses are the only viruses of this ... | 2012 | 23242303 |
| viruses acquired abroad: what does the primary care physician need to know? | viral infections are imported by travelers and immigrants from tropical or subtropical regions. the primary care physician should be able to include these diseases in the differential diagnosis of various clinical conditions. | 2012 | 23264811 |
| adaptive changes in alphavirus mrna translation allowed colonization of vertebrate hosts. | members of the alphavirus genus are arboviruses that alternate replication in mosquitoes and vertebrate hosts. in vertebrate cells, the alphavirus resists the activation of antiviral rna-activated protein kinase (pkr) by the presence of a prominent rna structure (downstream loop [dlp]) located in viral 26s transcripts, which allows an eif2-independent translation initiation of these mrnas. this article shows that dlp structure is essential for replication of sindbis virus (sinv) in vertebrate ce ... | 2012 | 22761388 |
| cell-based analysis of chikungunya virus e1 protein in membrane fusion. | chikungunya fever is a pandemic disease caused by the mosquito-borne chikungunya virus (chikv). e1 glycoprotein mediation of viral membrane fusion during chikv infection is a crucial step in the release of viral genome into the host cytoplasm for replication. how the e1 structure determines membrane fusion and whether other chikv structural proteins participate in e1 fusion activity remain largely unexplored. | 2012 | 22520648 |
| pathogenic characterization of a cervical lymph node derived from a patient with kawasaki disease. | kawasaki disease (kd) is the most common cause of multisystem vasculitis in childhood. although cervical lymphadenitis is one of the major symptoms in kd, lymph node biopsy is rarely performed, because kd is usually diagnosed by clinical symptoms. a cervical lymph node biopsy was taken from a girl aged 1 year and 8 months who had suspected lymphoma, but she was diagnosed with kd after the biopsy. the cervical lymph node specimen was analyzed with multivirus real-time pcr that can detect >160 vir ... | 2012 | 23071864 |
| genetic ablation of arginase 1 in macrophages and neutrophils enhances clearance of an arthritogenic alphavirus. | chikungunya virus (chikv) and ross river virus (rrv) cause a debilitating, and often chronic, musculoskeletal inflammatory disease in humans. macrophages constitute the major inflammatory infiltrates in musculoskeletal tissues during these infections. however, the precise macrophage effector functions that affect the pathogenesis of arthritogenic alphaviruses have not been defined. we hypothesized that the severe damage to musculoskeletal tissues observed in rrv- or chikv-infected mice would pro ... | 2012 | 22972923 |
| genomic characterization of a novel virus of the family tymoviridae isolated from mosquitoes. | the family tymoviridae comprises three plant virus genera, including tymovirus, marafivirus, and maculavirus, which are found in most parts of the world and cause severe agricultural losses. we describe a putatively novel member of the family tymoviridae, which is isolated from mosquitoes (culex spp.), referred to as cutlv. | 2012 | 22848363 |
| mayaro virus infection in amazonia: a multimodel inference approach to risk factor assessment. | arboviral diseases are major global public health threats. yet, our understanding of infection risk factors is, with a few exceptions, considerably limited. a crucial shortcoming is the widespread use of analytical methods generally not suited for observational data--particularly null hypothesis-testing (nht) and step-wise regression (swr). using mayaro virus (mayv) as a case study, here we compare information theory-based multimodel inference (mmi) with conventional analyses for arboviral infec ... | 2012 | 23071852 |
| drivers, dynamics, and control of emerging vector-borne zoonotic diseases. | emerging vector-borne diseases are an important issue in global health. many vector-borne pathogens have appeared in new regions in the past two decades, while many endemic diseases have increased in incidence. although introductions and emergence of endemic pathogens are often considered to be distinct processes, many endemic pathogens are actually spreading at a local scale coincident with habitat change. we draw attention to key differences between dynamics and disease burden that result from ... | 2012 | 23200503 |
| mayaro virus infection in traveler returning from amazon basin, northern peru. | 2012 | 22469145 | |
| serological evidence of flaviviruses and alphaviruses in livestock and wildlife in trinidad. | seroprevalence rates of selected arboviruses in animal populations in trinidad were determined using serum samples collected between 2006 and 2009 from horses (n=506), cattle (n=163), sheep (n=198), goats (n=82), pigs (n=184), birds (n=140), rodents (n=116), and other vertebrates (n=23). the sera were screened for antibodies to west nile virus (wnv), st. louis encephalitis virus (slev), ilheus virus (ilhv), bussuquara virus (bsqv), venezuelan equine encephalitis virus (veev), eastern equine ence ... | 2012 | 22989182 |
| mitochondrial bioenergetic alterations in mouse neuroblastoma cells infected with sindbis virus: implications to viral replication and neuronal death. | the metabolic resources crucial for viral replication are provided by the host. details of the mechanisms by which viruses interact with host metabolism, altering and recruiting high free-energy molecules for their own replication, remain unknown. sindbis virus, the prototype of and most widespread alphavirus, causes outbreaks of arthritis in humans and serves as a model for the study of the pathogenesis of neurological diseases induced by alphaviruses in mice. in this work, respirometric analys ... | 2012 | 22485151 |