Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| new bnyvv p25 variants in belgium. | rhizomania is a widespread viral plant disease of major importance in sugar beet cropping and breeding. it is caused by the beet necrotic yellow vein virus (bnyvv), a benyvirus transmitted by the soil inhabiting plasmodiophorid polymyxa betae. this vector also transmits other sugar beet virus such as beet virus q (bvq) and beet soil-borne virus (bsbv). despite identification of resistance genes, bnyvv remains a major constraint because of resistance-breaking events as well as its ability to surv ... | 2015 | 27141738 |
| full length genome sequence of polish isolate of beet soil-borne virus confirms low level of genetic diversity. | the complete nucleotide sequence of a polish isolate of beet soil-borne virus was determined for the first time. the genome organization was identical with those previously established for isolates from germany and china. a comparison of the polish isolate with others deposited in genbank revealed high level of nucleotide identity, about 98-100%, throughout the genome analyzed. the ratio between non-synonymous and synonymous substitutions was rather low suggesting a negative selective pressure. ... | 2009 | 19997656 |
| a full-length infectious clone of beet soil-borne virus indicates the dispensability of the rna-2 for virus survival in planta and symptom expression on chenopodium quinoa leaves. | for a better understanding of the functionality and pathogenicity of beet soil-borne virus (bsbv), full-length cdna clones have been constructed for the three genomic rnas. with the aim of assessing their effectiveness and relative contribution to the virus housekeeping functions, transcripts were inoculated on chenopodium quinoa and beta macrocarpa leaves using five genome combinations. both rnas-1 (putative replicase) and -3 (putative movement proteins) proved to be essential for virus replica ... | 2009 | 19726609 |
| efficient dsrna-mediated transgenic resistance to beet necrotic yellow vein virus in sugar beets is not affected by other soilborne and aphid-transmitted viruses. | rhizomania caused by beet necrotic yellow vein virus (bnyvv) is one of the most devastating sugar beet diseases. sugar beet plants engineered to express a 0.4 kb inverted repeat construct based on the bnyvv replicase gene accumulated the transgene mrna to similar levels in leaves and roots, whereas accumulation of the transgene-homologous sirna was more pronounced in roots. the roots expressed high levels of resistance to bnyvv transmitted by the vector, polymyxa betae. resistance to bnyvv was n ... | 2008 | 17431806 |
| sequence divergence of four soilborne sugarbeet-infecting viruses. | soilborne viruses are among the most harmful pathogens of sugarbeet (beta vulgaris l.ssp. vulgaris) but most of them lack information on genetic variability due to paucity of sequence data. only one isolate of beet soil borne virus (bsbv; genus pomovirus), beet virus q (bvq; genus pomovirus) and beet soil borne mosaic virus (bsbmv; genus benyvirus) has been characterised for the coat protein (cp) gene. in this study, the cp gene sequences of three isolates each of bsbv and beet necrotic yellow v ... | 2005 | 15965609 |
| development of a highly sensitive nested rt-pcr method for beet necrotic yellow vein virus detection. | a diagnostic test incorporating reverse-transcription polymerase chain reaction (rt-pcr) and nested polymerase chain reaction (npcr) was developed for the detection of beet necrotic yellow vein virus (bnyvv). the rt-pcr used the primers designed by (henry et al., j. virol. methods 54 (1995)15) but refinements were made to the protocol including simplification of the extraction method, the use of standard reagents and adoption of a one-step procedure. none of these changes impaired sensitivity or ... | 2001 | 11377723 |
| complete sequence of rna 1 and the presence of trna-like structures in all rnas of potato mop-top virus, genus pomovirus. | the complete nucleotide sequence (6043 nt) of rna 1 from potato mop-top virus (pmtv-sw), the type member of the genus pomovirus, was determined. the first (5'-terminal) open reading frame (orf 1) encodes a predicted protein of 148 kda. orf 2 extends through the opal stop codon of orf 1 producing a predicted readthrough protein of 206 kda which resembles the rna-dependent rna polymerases (rdrp) of other fungal-transmitted viruses. it includes a methyltransferase, a helicase and a gdd rdrp motif, ... | 1999 | 10573175 |
| transfer rna mimicry in a new group of positive-strand rna plant viruses, the furoviruses: differential aminoacylation between the rna components of one genome. | recent sequencing of the genomes of several furoviruses--fungus-transmitted rod-shaped positive-strand plant viruses--has suggested the presence of trna-like structures (tlss) at the 3' ends of the genomic rnas. we show here that the genomic rnas of soil-borne wheat mosaic virus (sbwmv), beet soil-borne virus (bsbv), potato mop-top virus (pmtv), peanut clump virus (pcv), and indian peanut clump virus (ipcv) all possess functional tlss that are capable of high-efficiency valylation. while the sbw ... | 1998 | 9657004 |
| beet soil-borne virus rna 2: similarities and dissimilarities to the coat protein gene-carrying rnas of other furoviruses. | the complete sequence of the 3454 nt of rna 2 of the ahlum isolate of beet soil-borne furovirus (bsbv) has been determined starting with two short stretches of cloned cdna. unknown parts of the sequence were amplified by means of rt-pcr techniques using combinations of specific and random primers. bsbv rna 2 is more similar in its genetic organization to potato mop top virus (pmtv) rna 3 than to any other furoviral rna, although it is more than 1100 nt longer. its 3'-end, unlike that of pmtv rna ... | 1997 | 9018071 |
| beet soil-borne virus rna 1: genetic analysis enabled by a starting sequence generated with primers to highly conserved helicase-encoding domains. | the complete sequence of the 5834 nucleotides of rna 1 of beet soil-borne furovirus (bsbv, ahlum isolate) was determined using a pcr product obtained with primers to highly conserved coding regions for helicase-like proteins in rna 1 of furo-, hordei- and tobraviruses as a starting sequence. unknown parts of the sequence upstream and downstream of this starting sequence were amplified by means of rt-pcr techniques using combinations of specific and random primers. bsbv rna 1 contains one large o ... | 1997 | 9400965 |
| beet soil-borne virus rna 3--a further example of the heterogeneity of the gene content of furovirus genomes and of triple gene block-carrying rnas. | the complete nucleotide sequence of rna 3 of the ahlum isolate of beet soil-borne virus (bsbv) was determined from cdnas obtained with immunocaptured virus particles and denatured preparations of dsrna. bsbv rna 3 is unique among the plant virus rnas studied so far in containing apparently only the coding sequences of a triple gene block (tgb). the derived amino acid sequences of the three putative tgb-encoded proteins showed the highest level of sequence similarities with those of the correspon ... | 1996 | 8614988 |
| single- and double-stranded rnas associated with an isolate of beet soil-borne virus. | ethidium bromide staining of electrophoretically separated ssrnas and dsrnas as well as northern blot analyses with cdna clones suggested that the genome of the ahlum serotype of beet soil-borne virus (bsbv) consists of two major ssrna species of approximately 3.6 and 3.2 kb, respectively, and possibly a minor ssrna of approximately 6.0 kb. a few of our clones hybridized with both the 3.6-kb and the 3.2-kb rnas, the majority of the clones, however, hybridized only with the 3.2-kb rna. the 3.2-kb ... | 1992 | 1568837 |
| a comparison, using dsrna analysis, between beet soil-borne virus and some other tubular viruses isolated from sugar beet. | double-stranded rna preparations from chenopodium quinoa leaves inoculated with two english isolates of beet soil-borne virus (bsbv), bsbv-n and bsbv-452n, a french isolate of beet necrotic yellow vein virus (bnyvv), a swedish isolate of a tubular beet virus (86-109) or a belgian isolate of a similar virus (1530) were compared following separation on non-denaturing polyacrylamide gels. the dsrnas of bnyvv differed in mobility from those isolated from tissue infected with the other four tubular b ... | 1992 | 1588330 |