Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
ectomycorrhization of tricholoma matsutake with quercus aquifolioides affects the endophytic microbial community of host plant.tricholoma matsutake (s. ito et imai) is an ectomycorrhizal basidiomycete associated with pinaceae and fagaceae trees in the northern hemisphere. it is still unknown whether the symbiotic relationship with this ectomycorrhiza could affect the host plant's endophytic microbial community. in this study, we used high throughput sequencing to analyze the endophytic microbial communities of different quercus aquifolioides tissues with or without t. matsutake partner. about 35,000 clean reads were obt ...201829359810
heavy-ion beam mutagenesis of the ectomycorrhizal agaricomycete tricholoma matsutake that produces the prized mushroom "matsutake" in conifer forests.tricholoma matsutake is an ectomycorrhizal agaricomycete that produces the prized mushroom "matsutake" in pinaceae forests. currently, there are no available cultivars or cultivation methods that produce fruiting bodies. heavy-ion beams, which induce mutations through double-stranded dna breaks, have been used widely for plant breeding. in the present study, we examined whether heavy-ion beams could be useful in isolating t. matsutake mutants. an argon-ion beam gave a suitable lethality curve in ...201829164316
artificial 137cs and natural 40k in mushrooms from the subalpine region of the minya konka summit and yunnan province in china.a study on 137cs pollution and activity concentrations of 40k in mushrooms of the genera cortinarius, leccinum, russula, tricholoma, tylopilus, and xerocomus from two neighboring regions in southwest china in 2010-2013 revealed different patterns of pollution with 137cs, which seemed to be highly dependent on climate conditions. tricholoma matsutake was collected in yunnan before and after the fukushima dai-ichi nuclear accident and showed similar contamination with 137cs. mushrooms from the ele ...201829052148
applied mycology can contribute to sustainable rural livelihoods: building upon china's matsutake management initiatives.matsutake mushrooms are an important part of rural livelihoods and forest ecosystems across large parts of china, as well as elsewhere in east asia, northern europe and north america. mushroom harvesters have developed sophisticated understandings of matsutake ecology and production, and are applying this knowledge in various innovative management strategies. at the same time, chinese government agencies and scientists are promoting matsutake-based livelihoods to support development and conserva ...201729273995
local-level genetic diversity and structure of matsutake mushroom (tricholoma matsutake) populations in nagano prefecture, japan, revealed by 15 microsatellite markers.the annual yield of matsutake mushrooms (tricholoma matsutake) has consistently decreased in japan over the past few decades. we used 15 polymorphic and codominant simple sequence repeat (ssr) markers, developed using next-generation sequencing, to carry out genetic analyses of 10 populations in nagano, japan. using the ssrs, we identified 223 genotypes, none of which was observed in more than one population. the mean expected heterozygosity and standardized allelic richness values were 0.67 and ...201729371541
structure elucidation and antitumor activity of a new polysaccharide from maerkang tricholoma matsutake.a new heteropolysaccharide was isolated from the fruiting bodies of tricholoma matsutake which had a molecular weight of 12078 da. the results of structural features analysis showed that t. matsutake polysaccharide, here named tmp-b, was mainly composed of α - d - glucose and α - d - galactose which ratios were 7:2 and had a backbone of 1, 4 - linked α - d - glucose which branches were mainly composed of two 6 - linked α - d - galactose residue, and the α - d - galactose was 1, 6 - linked. antit ...201728808425
ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from tricholoma matsutake.an ultrasonic-assisted technique was employed to extract crude polysaccharide from tricholoma matsutake fruiting bodies. single-factor tests and orthogonal experimental design (l9(3(3))) were used to obtain the optimal extraction conditions. results showed that the optimal parameters were as follows: ultrasonic temperature, 40 °c; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 khz; and ultrasonic power, 100 w. three novel t. matsutake polysaccharide (tmp) ...201728786242
investigation of the immunomodulatory activity of tricholoma matsutake mycelium in cyclophosphamide-induced immunosuppressed mice.tricholoma matsutake, a popular food and biopharmaceutical resource in asia, possesses various pharmacological activities. although t. matsutake mycelium (tm) may enhance immunity, previous studies, to the best of our knowledge, have been performed on normal animals or cells alone. the present study aimed to evaluate the immunomodulatory activity of tm at doses of 0.3, 1.0 and 2.0 g/kg in cyclophosphamide (ctx)‑induced immunosuppressed mouse models. tm treatment for 2 weeks markedly improved the ...201728765877
matsutakone and matsutoic acid, two (nor)steroids with unusual skeletons from the edible mushroom tricholoma matsutake.matsutakone (1), a novel sterol with an unprecedented polycyclic ring system, together with a new norsteroid matsutoic acid (2) were isolated from the fruiting bodies of tricholoma matsutake. their structures and absolute configurations were assigned by extensive spectroscopic analyses and computational methods. bioassay results showed that compounds 1 and 2 exhibited inhibitory activities against acetylcholinesterase (ic50 20.9 μm for 1).201728691489
north american matsutake: names clarified and a new species described.tricholoma matsutake, known widely as "matsutake," has great commercial and cultural significance in japan. because japanese production is insufficient to meet the high domestic demand, morphologically similar mushrooms, thought by many to belong to t. magnivelare, are imported from western north america. however, molecular data produced since the early 2000s have indicated that more than one species of matsutake occur in north america and this raises the question of correct naming for the diffe ...201728609221
effects of matsutake mushroom scent compounds on tyrosinase and murine b16-f10 melanoma cells.tyrosinase-catalyzed l-tyrosine oxidation is a key step in melanogenesis, and intense melanin formation is often a problem in chemotherapies or food preservation. here we report that methyl cinnamate one of the constituents characterized from mycelium and sporocarp of american matsutake mushroom tricholoma magnivelare inhibits both enzymatic and cellular melanin formation. methyl cinnamate inhibits mushroom tyrosinase-catalyzed l-tyrosine oxidation while the oxidation of l-3,4-dihydroxyphenylala ...201728456625
prediction of the potential geographic distribution of the ectomycorrhizal mushroom tricholoma matsutake under multiple climate change scenarios.effective conservation and utilization strategies for natural biological resources require a clear understanding of the geographic distribution of the target species. tricholoma matsutake is an ectomycorrhizal (ecm) mushroom with high ecological and economic value. in this study, the potential geographic distribution of t. matsutake under current conditions in china was simulated using maxent software based on species presence data and 24 environmental variables. the future distributions of t. m ...201728393865
the (oxalato)aluminate complex as an antimicrobial substance protecting the "shiro" of tricholoma matsutake from soil micro-organisms.tricholoma matsutake, a basidiomycete, forms ectomycorrhizas with pinus densiflora as the host tree. its fruiting body, "matsutake" in japanese, is an edible and highly prized mushroom, and it grows in a circle called a fairy ring. beneath the fairy ring of t. matsutake, a whitish mycelium-soil aggregated zone, called "shiro" in japanese, develops. the front of the shiro, an active mycorrhizal zone, functions to gather nutrients from the soil and roots to nourish the fairy ring. bacteria and spo ...201727691719
a qpcr assay that specifically quantifies tricholoma matsutake biomass in natural soil.tricholoma matsutake is an ectomycorrhizal basidiomycete that produces prized, yet uncultivable, "matsutake" mushrooms along densely developed mycelia, called "shiro," in the rhizosphere of coniferous forests. pinus densiflora is a major host of this fungus in japan. measuring t. matsutake biomass in soil allows us to determine the kinetics of fungal growth before and after fruiting, which is useful for analyzing the conditions of the shiro and its surrounding mycorrhizosphere, predicting fruiti ...201627371100
comparative analysis of macrophage transcriptomes reveals a key mechanism of the immunomodulatory activity of tricholoma matsutake polysaccharide.in the present study, we performed a proliferation assay, phagocytosis assay and cell cycle analysis of macrophages and sequenced the transcriptomes of control group macrophages and tmp-a group macrophages using illumina sequencing technology to identify differentially expressed genes (degs) and determine the molecular mechanisms associated with differences in the immunomodulatory activity of tmp-a in macrophages. the results showed that tmp-a exhibits strong proliferation activity and phagocyto ...201627221808
a tricholoma matsutake peptide with angiotensin converting enzyme inhibitory and antioxidative activities and antihypertensive effects in spontaneously hypertensive rats.hypertension is a major risk factor for cardiovascular disease. a crude water extract of the fruiting bodies of a highly prized mushroom tricholoma matsutakei exerted an antihypertensive action on spontaneously hypertensive rats (shrs) at a dosage of 400 mg/kg. an angiotensin converting enzyme (ace) inhibitory peptide with an ic50 of 0.40 μm was purified from the extract and designated as tmp. its amino acid sequence was elucidated to be walkgyk through lc-ms/ms analysis. the lineweaver-burk plo ...201627052674
tricholoma matsutake aqueous extract induces hepatocellular carcinoma cell apoptosis via caspase-dependent mitochondrial pathway.tricholoma matsutake, one of widely accepted functional mushrooms, possesses various pharmacological activities, and its antitumor effect has become an important research point. our study aims to evaluate the cytotoxicity activities of t. matsutake aqueous extract (tm) in hepg2 and smmc-7721 cells. in in vitro experiments, tm strikingly reduced cell viability, promoted cell apoptosis, inhibited cell migration ability, induced excessive generation of ros, and caused caspases cascade and mitochond ...201628018916
complete mitochondrial genome of the ectomycorrhizal fungus tricholoma matsutake.tricholoma matsutake is an ectomycorrhizal fungus belonging to the class agaricomycetes and the phylum basidiomycota. here, we report the complete mitochondrial genome sequence of t. matsutake. the mitochondrial dna (mtdna) of t. matsutake seems to be concatenated, and harbors 76,037 nucleotides, with an overall gc content of 20.69%. the mtdna comprises 15 protein-coding genes, 28 trna genes, and 2 rrna genes. a comparative mitogenomic analysis between agaricomycetes and other classes (exobasidi ...201625208172
distinctive feature of microbial communities and bacterial functional profiles in tricholoma matsutake dominant soil.tricholoma matsutake, the pine mushroom, is a valuable forest product with high economic value in asia, and plays an important ecological role as an ectomycorrhizal fungus. around the host tree, t. matsutake hyphae generate a distinctive soil aggregating environment called a fairy ring, where fruiting bodies form. because t. matsutake hyphae dominate the soil near the fairy ring, this species has the potential to influence the microbial community. to explore the influence of t. matsutake on the ...201627977803
analysis of bacterial diversity and communities associated with tricholoma matsutake fruiting bodies by barcoded pyrosequencing in sichuan province, southwest china.endophytes play an important role in the growth and development of the host. however, the study of endophytes is mostly focused on plants, and reports on bacteria associated with fungi are relatively rare. we studied the bacteria associated with fruiting bodies of tricholoma matsutake picked from seven main t. matsutake-producing areas in sichuan, china, by barcoded pyrosequencing. about 8,272 reads were obtained per sample, representing 40 phyla, 103 classes, and 495 genera of bacteria and arch ...201626428733
isolation and structural elucidation of a novel homogenous polysaccharide from tricholoma matsutake.a crude polysaccharide possessing antitumour, radiation-resistant and anti-ageing attributes was extracted from tricholoma matsutake by water extraction and alcohol precipitation. from this crude polysaccharide, a homogeneous polysaccharide, tmp-5ii, was successfully purified by sephacryl s-300 column chromatography. the average molecular weight (mw) of tmp-5ii was 15.76 kda. monosaccharide analysis indicated that the homogeneous polysaccharide contained four different residues: d-glucose, d-gal ...201626208132
a fungal α-galactosidase from tricholoma matsutake with broad substrate specificity and good hydrolytic activity on raffinose family oligosaccharides.an acidic α-galactosidase designated as tmg was purified from the fruiting bodies the purification protocol entailed ion exchange chromatography on q-sepharose and of tricholoma matsutake with 136-fold purification and a specific activity of 909 units/mg. mono-q and fast protein liquid chromatography on superdex 75. tmg is a monomeric protein exhibiting a molecular mass of 47 kda in sds-page and gel filtration. the purified enzyme was identified by lc-ms/ms and three inner amino acid sequences w ...201526213909
tricholoma matsutake fruit bodies secrete hydrogen peroxide as a potent inhibitor of fungal growth.tricholoma matsutake is an ectomycorrhizal fungus that dominates the microbial communities in the soil of pine and spruce forests. the mycorrhizas of this fungus have antimicrobial activity, although factors responsible for the antimicrobial activity have not been fully elucidated. the present study shows that fruit bodies of t. matsutake secreted hydrogen peroxide (h2o2), which was produced by pyranose oxidase, and that the h2o2 thus secreted strongly inhibited the growth of mycelia of the phyt ...201525803209
a novel laccase from fresh fruiting bodies of the wild medicinal mushroom tricholoma matsutake.the knowledge about biological activities of constituents from medicinal mushrooms belonging to the genus tricholoma is limited. a 59-kda laccase has now been purified from fresh fruiting bodies of the mushroom tricholoma matsutake. the purification protocol entailed ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on cm-cellulose, affinity chromatography on cona-sepharose, and gel filtration by fast protein liquid chromatog ...201525781157
genetic variability and bottleneck detection of four tricholoma matsutake populations from northeastern and southwestern china.the excessive commercial collection of matsutake mushrooms can lead to extreme reduction of population size, which may cause genetic bottleneck and decrease genetic diversity of tricholoma matsutake. here, six polymorphic microsatellite loci markers were used to examine the genetic diversity of four natural t. matsutake populations from two main producing regions of china. the minimum combinations of four loci were able to discriminate total 86 sampled individuals with distinctive multilocus gen ...201525682708
ectomycorrhizas in vitro between tricholoma matsutake, a basidiomycete that associates with pinaceae, and betula platyphylla var. japonica, an early-successional birch species, in cool-temperate forests.tricholoma matsutake is an ectomycorrhizal basidiomycete that associates with pinaceae in the northern hemisphere and produces prized "matsutake" mushrooms. we questioned whether the symbiont could associate with a birch that is an early-successional species in boreal, cool-temperate, or subalpine forests. in the present study, we demonstrated that t. matsutake can form typical ectomycorrhizas with betula platyphylla var. japonica; the associations included a hartig net and a thin but distinct f ...201525236465
tricholoma matsutake can absorb and accumulate trace elements directly from rock fragments in the shiro.tricholoma matsutake, a highly valued delicacy in japan and east asia, is an ectomycorrhizal fungus typically found in a complex soil community of mycorrhizae, soil microbes, and host-tree roots referred to as the shiro in japan. a curious characteristic of the shiro is an assortment of small rock fragments that have been implicated as a direct source of minerals and trace elements for the fungus. in this study, we measured the mineral content of 14 samples of shiro soil containing live matsutak ...201525355073
cloning and expression analysis of phenylalanine ammonia-lyase gene in the mycelium and fruit body of the edible mushroom flammulina velutipes.phenylalanine ammonia-lyase (pal) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. however, its expression in fungi remains to be explored. to understand its expression in mushroom fungi, the pal gene of the edible mushroom flammulina velutipes (fvpal) was cloned and characterized. the cloned fvpal consists of 2,175 bp, coding for a polypeptide containing 724 amino acids and having 11 introns. the translated amino aci ...201526539050
variation among matsutake ectomycorrhizae in four clones of pinus sylvestris.tricholoma matsutake is an ectomycorrhizal fungus that forms commercially important mushrooms in coniferous forests. in this study, we explored the ability of t. matsutake to form mycorrhizae with pinus sylvestris by inoculating emblings produced through somatic embryogenesis (se) in an aseptic culture system. two months after inoculation, clones with less phenolic compounds in the tissue culture phase formed mycorrhizae with t. matsutake, while clones containing more phenols did not. effects of ...201525179801
antifatigue activity of liquid cultured tricholoma matsutake mycelium partially via regulation of antioxidant pathway in mouse.tricholoma matsutake has been popular as food and biopharmaceutical materials in asian countries for its various pharmacological activities. the present study aims to analyze the antifatigue effects on enhancing exercise performance of tricholoma matsutake fruit body (abm) and liquid cultured mycelia (tm) in mouse model. two-week tricholoma matsutake treatment significantly enhances the exercise performance in weight-loaded swimming, rotating rod, and forced running test. in tm- and abm-treated ...201526697489
the host ranges of conifer-associated tricholoma matsutake, fagaceae-associated t. bakamatsutake and t. fulvocastaneum are wider in vitro than in nature.tricholoma matsutake is the most commercially important edible mushroom in pine forests in japan. tricholoma bakamatsutake and t. fulvocastaneum, species closely related to t. matsutake, occur in fagaceae forests. we examined ectomycorrhizal (em) formation by these tricholoma species by in vitro synthesis among seven strains (two of t. matsutake, four of t. bakamatsutake, one of t. fulvocastaneum) and axenic plants of pine (pinus densiflora) and oak (quercus serrata, q. phillyraeoides). all stra ...201424871598
root endophyte symbiosis in vitro between the ectomycorrhizal basidiomycete tricholoma matsutake and the arbuscular mycorrhizal plant prunus speciosa.we previously reported that tricholoma matsutake and tricholoma fulvocastaneum, ectomycorrhizal basidiomycetes that associate with pinaceae and fagaceae, respectively, in the northern hemisphere, could interact in vitro as a root endophyte of somatic plants of cedrela odorata (meliaceae), which naturally harbors arbuscular mycorrhizal fungi in south america, to form a characteristic rhizospheric colony or "shiro". we questioned whether this phenomenon could have occurred because of plant-microbe ...201424158697
polysaccharides from tricholoma matsutake and lentinus edodes enhance 5-fluorouracil-mediated h22 cell growth inhibition.few studies have investigated the effects produced by combinations of polysaccharides and chemotherapeutic drugs in cancer treatment. we hypothesized that a combination of polysaccharides (cop) from lentinus edodes and tricholoma matsutake would improve the efficacy of 5-fluorouracil (5-fu)-mediated inhibition of h22 cell growth.201424992758
extraction and purification of polysaccharides from pine medicinal mushroom, tricholoma matsutake (higher basidiomycetes) fruit bodies.ultrasound-assisted extraction, purification, and characterization of antioxidant polysaccharides from tricholoma matsutake were carried out. on the basis of the results of a single-factor test, three independent and main variables, including extraction time (x1: 130-170 s), ultrasonic power (x2: 340-380 w), and ratio of water to raw material (x3: 45-55 ml/g) were studied by box-behnken design. the optimum extraction conditions are as follows: extraction time 160 s, ultrasonic power 365 w, and r ...201424941036
comparative analysis of bacterial diversity and communities inhabiting the fairy ring of tricholoma matsutake by barcoded pyrosequencing.comparative analysis of the soil bacterial communities inhabiting the fairy ring of tricholoma matsutake.201424922184
a comparison study on extraction of polysaccharides from tricholoma matsutake by response surface methodology.the tricholoma matsutake polysaccharides (tmp) extraction was optimized using counter-current probe ultrasonic extraction (ccpue) by response surface methodology (rsm). the effect of three factors (liquid-solid ratio, extraction time and pulse duration) on the tmp yield was studied. the results showed that the optimal conditions were liquid-solid ratio (15), extraction time (61 min) and pulse duration (6.0 s). under best conditions, the maximum tmp yield was 8.11 ± 0.35%, which was close with th ...201424507300
detection of the edible ectomycorrhizal fungus lyophyllum shimeji colonising seedlings of cultivated conifer species in new zealand.lyophyllum shimeji is an edible ectomycorrhizal fungus that is widely distributed in east asia and also present in the northern regions of europe. in japan, l. shimeji is a culinary delicacy, considered amongst all edible mushrooms to have the best taste and to be second only to tricholoma matsutake in price. traditionally, fruiting bodies of l. shimeji have been collected from the wild but fruiting of l. shimeji is now relatively uncommon and cannot keep up with increasing consumer demand. as a ...201424445577
terpenoids and sterols from some japanese mushrooms.over the past twenty years, our research group has been studying the chemical constituents of mushrooms. from nineteen species, namely, amanita virgineoides bas (amanitaceae), daedaleopsis tricolor (bull.: fr.) bond. et sing. (polyporaceae), grifolafrondosa (fr.) s. f. gray (polyporaceae), hericium erinaceum (bull.: fr.) pers. (hericiaceae), hypsizigus marmoreus (peck) bigelow (tricholomataceae), lactarius piperatus (scop.: fr.) s. f. gray (russulaceae), lentinula edodes (berk.) sing. (pleurotac ...201424689228
trichoderma songyi sp. nov., a new species associated with the pine mushroom (tricholoma matsutake).a new species, trichoderma songyi, was found to be associated with the pine mushroom (tricholoma matsutake) in korea. this species was isolated from three different substrates: tricholoma matsutake basidiomata, as well as roots of pinus densiflora and soil in the fairy ring. based on its molecular and phenotypic characteristics, we demonstrate that trichoderma songyi is unique and distinguishable from closely related species. we performed phylogenetic analyses based on two molecular markers, the ...201425052534
extract of the mycelium of t. matsutake inhibits elastase activity and tpa-induced mmp-1 expression in human fibroblasts.skin aging is induced through complex biological processes in human skin caused by proteolysis of collagen and elastin, two structural proteins of the dermal extracellular matrix (ecm). collagen and elastin degradation can induce the expression of matrix metalloproteinases (mmps), as well as that of a family of zinc-dependent endopeptidases that play critical roles in skin aging. moreover, elastase is a metalloproteinase which acts on the degradation of elastin in skin aging, and is also involve ...201425319362
[community structure and diversity of entophytic bacteria in tricholoma matsutake in sichuan province, southwest china].in order to understand the diversity and community structure of endophytic bacteria in tricholoma matsutake, 14 tricholoma matsutake samples were collected from 7 main production counties of xiaojin, yajiang, muli, yanyuan, yanbian, huidong and mianning. the entophytic bacterial community structure and diversity were investigated by pcr denaturing gradient gel electrophoresis (pcr-dgge). the results showed that the diversity and community structure of endophytic bacteria in t. matsutake varied s ...201425898631
strategies of functional food for cancer prevention in human beings.functional food for prevention of chronic diseases is one of this century's key global challenges. cancer is not only the first or second leading cause of death in china and other countries across the world, but also has diet as one of the most important modifiable risk factors. major dietary factors now known to promote cancer development are polished grain foods and low intake of fresh vegetables, with general importance for an unhealthy lifestyle and obesity. the strategies of cancer preventi ...201323679240
anti-microorganism, anti-tumor, and immune activities of a novel polysaccharide isolated from tricholoma matsutake.many more fungal polysaccharides have been reported to exhibit a variety of biological activities, including anti-tumor, immunostimulation, anti-oxidation, and so on. the non-starch polysaccharides have emerged as an important class of bioactive natural products.201323930009
cloning and mrna expression analysis of the gene encoding phenylalanine ammonia-lyase of the ectomycorrhizal fungus tricholoma matsutake.the ectomycorrhizal fungus tricholoma matsutake grows symbiotically with pinus densiflora. phenylalanine ammonia-lyase (e.c. 4.3.1.24) catalyzes the conversion of l-phenylalanine to trans-cinnamic acid. the role of fungal phenylalanine ammonia-lyase, however, has not been clear until now. in this study, the gene encoding phenylalanine ammonia-lyase (pal), which was isolated from t. matsutake, was cloned and characterized. the pal gene (tmpal) consists of 2,160 nucleotides, coding for a polypepti ...201323801251
mobile dna distributions refine the phylogeny of "matsutake" mushrooms, tricholoma sect. caligata."matsutake" mushrooms are formed by several species of tricholoma sect. caligata distributed across the northern hemisphere. a phylogenetic analysis of matsutake based on virtually neutral mutations in dna sequences resolved robust relationships among tricholoma anatolicum, tricholoma bakamatsutake, tricholoma magnivelare, tricholoma matsutake, and tricholoma sp. from mexico (=tricholoma sp. mex). however, relationships among these matsutake and other species, such as tricholoma caligatum and tr ...201323440576
identification and analysis of the constituents in an aqueous extract of tricholoma matsutake by hplc coupled with diode array detection/electrospray ionization mass spectrometry.the main constituents in an aqueous extract of tricholoma matsutake (tm) were identified by high-performance liquid chromatography coupled with diode array detection and electrospray ionization time-of-flight mass spectrometry (hplc-dad/tof-ms) and ion trap mass spectrometry (hplc-dad/trap-msn). the main factors in the extraction process which affect the yields of nutrients were optimized by single-factor experiments and orthogonal experiment design. in total, 12 constituents were identified fro ...201323957403
metagenomic analysis of fungal communities inhabiting the fairy ring zone of tricholoma matsutake.tricholoma matsutake, an ectomycorrhiza that has mutual relationships with the rootlet of pinus denisflora, forms a fruiting body that serves as a valuable food in asia. however, the artificial culture of this fungus has not been successful. soil fungi, including t. matsutake, coexist with many other microorganisms and plants; therefore, complex microbial communities have an influence on the fruiting body formation of t. matsutake. here, we report on the structures of fungal communities associat ...201323949332
calcium sensitizers isolated from the edible pine mushroom, tricholoma matsutake (s. ito & imai) sing.three lactam compounds were isolated from the fruiting body of tricholoma matsutake (s. ito & imai) sing., an edible mushroom, and their structures were identified as cyclo-s-proline-r-leucine (1), hexahydro-2h-azepin-2-one (2), and butyl 5-oxo-2-pyrrolidine carboxylate (3) by chemical, physicochemical, and spectral evidence. in in vitro screening tests, compounds 1 and 2 acted as calcium sensitizers in ventricular cells from rat. further studies on compounds 1 and 2 in ex vivo isolated right at ...201323819306
structural characterisation of polysaccharides from tricholoma matsutake and their antioxidant and antitumour activities.in this study, polysaccharides from tricholoma matsutake (tm-p) were purified using a deae-sepharose fast flow column and three polysaccharide fractions (tm-p1, tm-p2 and tm-p3) were obtained. the chemical composition and structural characteristics of tm-p2 were quite different from those of tm-p1 and tm-p3. tm-p2 consisted of glucose, galactose and mannose with a molar ratio of 5.9:1.1:1.0. the glycosidic linkages were mainly composed of 1,6- and 1-linked glucose. furthermore, tm-p2 showed the ...201323497882
purification, chemical characterization and radical scavenging activities of alkali-extracted polysaccharide fractions isolated from the fruit bodies of tricholoma matsutake.tricholoma matsutake, a high-class edible mushroom in china, has been regarded as famous foods and biopharmaceutical materials with a great deal of interest. in the previous investigations, researchers believe the water-soluble polysaccharide β-glucan is the major active component of t. matsutake, which displays various biological activities. in the present study, two novel alkali-extracted polysaccharide fractions, tm-aps-1 and tm-aps-2, were isolated from the fruit bodies of t. matsutake by de ...201323242547
root endophyte interaction between ectomycorrhizal basidiomycete tricholoma matsutake and arbuscular mycorrhizal tree cedrela odorata, allowing in vitro synthesis of rhizospheric "shiro".the ectomycorrhizal basidiomycete tricholoma matsutake associates with members of the pinaceae such as pinus densiflora (red pine), forming a rhizospheric colony or "shiro," which produces the prized "matsutake" mushroom. we investigated whether the host specificity of t. matsutake to conifers is innately determined using somatic plants of cedrela odorata, a tropical broad-leaved tree (meliaceae) that naturally harbors arbuscular mycorrhizal fungi. we found that t. matsutake could form in vitro ...201323064771
detecting nonculturable bacteria in the active mycorrhizal zone of the pine mushroom tricholoma matsutake.the fungus tricholoma matsutake forms an ectomycorrhizal relationship with pine trees. its sporocarps often develop in a circle, which is commonly known as a fairy ring. the fungus produces a solid, compact, white aggregate of mycelia and mycorrhizae beneath the fairy ring, which in japanese is called a 'shiro'. in the present study, we used soil dilution plating and molecular techniques to analyze the bacterial communities within, beneath, and outside the t. matsutake fairy ring. soil dilution ...201222538647
immunomodulatory activities of different solvent extracts from tricholoma matsutake (s. ito et s. imai) singer (higher basidiomycetes) on normal mice.the immunomodulatory activities of different solvent extracts from the culinary-medicinal mushroom tricholoma matsutake were studied in vivo in normal mice. the extracts were prepared using different solvents in an order of increasing polarity. the immunomodulatory activities were investigated by measuring the thymus and spleen index, phagocytic rate of macrophage phagocytosis, delayed-type hypersensitivity, plaque-forming cell, and proliferation of splenocytes. results demonstrated that water e ...201223510248
the ectomycorrhizal fungus tricholoma matsutake is a facultative saprotroph in vitro.tricholoma matsutake is an economically important ectomycorrhizal fungus of coniferous woodlands. mycologists suspect that this fungus is also capable of saprotrophic feeding. in order to evaluate this hypothesis, enzyme and chemical assays were performed in the field and laboratory. from a natural population of t. matsutake in southern finland, samples of soil-mycelium aggregate (shiro) were taken from sites of sporocarp formation and nearby control (pcr-negative) spots. soil organic carbon and ...201222005782
phylogenetic relationship and species delimitation of matsutake and allied species based on multilocus phylogeny and haplotype analyses.tricholoma matsutake (s. ito & s. imai) singer and its allied species are referred to as matsutake worldwide and are the most economically important edible mushrooms in japan. they are widely distributed in the northern hemisphere and established an ectomycorrhizal relationship with conifer and broadleaf trees. to clarify relationships among t. matsutake and its allies, and to delimit phylogenetic species, we analyzed multilocus datasets (its, megb1, tef, gpd) with samples that were correctly id ...201222684294
mineral element levels in wild edible mushrooms from yunnan, china.ten species of wild edible mushrooms (boletus griseus, boletus speciosus, lactarius hygrophoroides, leucopaxillus giganteus, macrocybe gigantea, melanoleuca arcuata, morchella deliciosa, mycena haematopus, pulveroboletus ravenelii, and tricholoma matsutake) collected from yunnan province of china, were analyzed for ten mineral elements (calcium, chromium, cobalt, copper, iron, magnesium, manganese, potassium, sodium, and zinc) contents using icp-aes. the minimum and maximum element contents of m ...201222234825
bak is a key molecule in apoptosis induced by methanol extracts of codonopsis lanceolata and tricholoma matsutake in hsc-2 human oral cancer cells.since the 5-year survival rate of oral cancer remains low, more effective and non-toxic therapeutic and preventive strategies are required. certain natural products possess anti-cancer properties. the present study investigated the effects of the methanol extracts of codonopsis lanceolata (meci) and tricholoma matsutake (metm) and identified the molecular target in hsc-2 human oral cancer cells. the results revealed that meci and metm inhibited growth and induced apoptosis, as demonstrated by po ...201223205139
tricholoma matsutake dominates diverse microbial communities in different forest soils.fungal and actinobacterial communities were analyzed together with soil chemistry and enzyme activities in order to profile the microbial diversity associated with the economically important mushroom tricholoma matsutake. samples of mycelium-soil aggregation (shiro) were collected from three experimental sites where sporocarps naturally formed. pcr was used to confirm the presence and absence of matsutake in soil samples. pcr-denaturing gradient gel electrophoresis (dgge) fingerprinting and dire ...201121984247
purification and properties of a nuclease from the fruit body of tricholoma matsutake.a nuclease was purified from the fruit body of tricholoma matsutake. the molecular mass was 38 kda. the optimum ph of the nuclease was about 8.0. its activity was inhibited by gtp. the nuclease cleaved rna and heat-denatured dna endonucleolytically to produce 5'-mononucleotide, and showed 3'-nucleotidase activity. the amino acid sequence up to seven amino acids from the n-terminus was nh(2)-apppssn.201020057129
phylogeography and evolution in matsutake and close allies inferred by analyses of its sequences and aflps.matsutake are commercially important ectomycorrhizal basidiomycetes in the genus tricholoma. despite their importance, the systematics of this species complex have remained elusive and little is known about their origin and biogeography. using dna analyses on a worldwide sample of matsutake, we present here the first comprehensive definition of natural groupings in this species complex. we infer patterns of migration and propose eocene origins for the group in western north america by a transfer ...201021148894
in vitro ectomycorrhizal specificity between the asian red pine pinus densiflora and tricholoma matsutake and allied species from worldwide pinaceae and fagaceae forests.tricholoma matsutake produces commercially valuable, yet uncultivable, mushrooms (matsutake) in association with pines in the far east and scandinavia and with both pines and oaks in the foothills of tibet. other matsutake mushrooms, such as tricholoma anatolicum from the mediterranean regions and tricholoma magnivelare and tricholoma sp. from the north pacific coast area of canada and north america as well as mexico, respectively, are associated with pines or oaks in their natural habitats. tri ...201019941149
ectomycorrhization of tricholoma matsutake and two major conifers in finland-an assessment of in vitro mycorrhiza formation.this study aimed to test the ability of tricholoma matsutake isolates to form mycorrhizas with aseptic seedlings of pinus sylvestris l. and picea abies (l.) karst. germinated seedlings of scots pine and norway spruce were separately inoculated with either isolates originating from finland or japan. eight months after inoculation, the finnish isolate had formed a sheath and hartig net on both host species. ectomycorrhizal scots pine seedlings inoculated with the finnish isolate showed the same sh ...201020177716
[optimization of ultrasonic-assisted extraction conditions of polysaccharides from tricholoma matsutake in meng mountain by response surface methodology].to obtain the optimal conditions of ultrasonic-assisted extraction of polysaccharides from fruitbody of tricholoma matsutake in meng mountain, and to provide the theoretical foundation for the further processing and utilization of tricholoma matsutake in meng mountain.201021137374
a case of anaphylactic reaction following matsutake mushroom ingestion: demonstration of histamine release reaction of basophils.matsutake mushroom is not recognized as a common food allergen. however, several case reports have suggested that this mushroom can induce anaphylaxis on rare occasions.201020864796
spore dispersion of tricholoma matsutake at a pinus densiflora stand in korea.the spore of tricholoma matsutake is considered to be the starting point of the mushroom growth cycle, but the mechanism of mycelial development from the spore stage is not yet clarified. in this study, we tried to measure how far the spores of t. matsutake disperse from a fruiting body located at a pinus densiflora stand in korea. we established 16 slide glasses coated with glycerin near a fruiting body in four directions separated by four different distance intervals within a mushroom producti ...201023956655
structure elucidation and antioxidant activity of a novel polysaccharide isolated from tricholoma matsutake.in this study, structural features of tricholoma matsutake polysaccharide (tmp-a) were investigated by a combination of infrared (ir) spectra, gas chromatography-mass spectrometry (gc-ms), nuclear magnetic resonance (nmr) spectroscopy. the results indicated that tmp-a had a backbone of 1,4-beta-d-glucopyranose residue which branches at o-6 based on the experimental results. the branches were mainly composed of an (1-->3)-alpha-d-galactopyranose residue, and terminated with alpha-d-xylopyranose r ...201020430053
process development for mycelial growth and polysaccharide production in tricholoma matsutake liquid culture.in this study, the effects of agitation and aeration on mycelial growth and exo-polysaccharide production were examined in batch cultures of tricholoma matsutake. agitation was varied from 100 to 300 rpm and aeration was varied from 0.5 to 1.5 vvm. mycelial growth was 21.87 g/l at 150 rpm, and exo-polysaccharide production was 8.79 g/l at 1.5 vvm. when we analyzed the polysaccharide extractions from the cultured mycelium and the culture broth of t. matsutake, 1.4 g of crude polysaccharide was fo ...201020226376
functional activation of macrophages, monocytes and splenic lymphocytes by polysaccharide fraction from tricholoma matsutake.mushroom-derived polysaccharides (beta-glucans) are considered as a valuable biopharmaceutical principle without displaying side effects. although tricholoma matsutake is well-known mushroom in korea, japan and china, the immunoregulatory roles of t. matsutake-derived polysaccharides were not fully elucidated yet. in this study, we continued to evaluate the immunomodulatory effect of t. matsutake-derived polysaccharide fraction (tmc-2) using functional activation models of macrophages, monocytes ...200920091269
association analysis of food allergens.food allergy patients are known to present with allergic reactions to multiple allergens, but extrapolating these associations is difficult. data mining, a procedure that analyzes characteristic combinations among large amounts of information, is often used to analyze and predict consumer purchasing behaviour. we applied this technique to the extrapolation of food allergen associations in allergy patients. we sent 1510 families our 'questionnaire survey for the prevention of food allergies'. res ...200919538355
development of specific primer for tricholoma matsutake.in this study, in an effort to develop a method for the molecular detection of tricholoma matsutake in korea from other closely related tricholomataceae, a species-specific pcr primer pair, tmf and tmr, was designed using nuclear ribosomal intertranscribed spacer (its) sequences. the dtmf and dtmr sequences were 5'-cctgacgccaatcttttca-3' and 5'-ggagagcagacttgtgagca-3', respectively. the pcr primers reliably amplified only the its sequences of t. matsutake, and not those of other species used in ...200923983557
forest age correlates with fine-scale spatial structure of matsutake mycorrhizas.examining the fine-scale spatial structure of fungal populations can tell us much about how individual species reproduce and disperse throughout natural landscapes. here we study the fine-scale genetic structure of tricholoma matsutake, a prized edible and medicinal mushroom, by systematic sampling of mycorrhizas within fairy rings in 50-y-old and old-growth forests in two villages. using single nucleotide polymorphism dna markers we show that mycorrhizas in both forest age classes in both villa ...200919284979
ergosterol and water changes in tricholoma matsutake soil colony during the mushroom fruiting season.the purpose of this study is to understand spatio-temporal changes of active fungal biomass and water in tricholoma matsutake soil colonies during the mushroom fruiting season. the active fungal biomass was estimated by analyzing ergosterol content at four different points within four replicated locations in a single circular t. matsutake colony at ssanggok valley in the sogri mt. national park in korea during 2003 to 2005. the four points were the ahead of the colony, the front edge of the colo ...200923983500
iridoid glucoside, (3r)-oct-1-en-3-ol glycosides, and phenylethanoid from the aerial parts of caryopteris incana.eleven compounds of interest were isolated from the aerial parts of caryopteris incana, specifically a new acyl derivative (3) of 8-o-acetylharpagide, two new (3r)-oct-1-en-3-ol glycosides (5, 6), and 6-o-caffeoylphlinoside a (11) along with seven known compounds, 8-o-acetylharpagide (1), 6'-o-p-coumaroyl-8-o-acetylharpagide (2), (3r)-oct-1-en-3-ol (matsutake alcohol) o-alpha-l-arabinopyranosyl-(1'' --> 6')-o-beta-d-glucopyranoside (4), apigenin 7-o-neohesperidinoside (7), 6'-o-caffeoylarbutin ( ...200919238325
recombination and genetic differentiation among natural populations of the ectomycorrhizal mushroom tricholoma matsutake from southwestern china.effective conservation and utilization strategies for natural biological resources require a clear understanding of the natural populations of the target organisms. tricholoma matsutake is an ectomycorrhizal mushroom that forms symbiotic associations with plants and plays an important ecological role in natural forest ecosystems in many parts of the world. it is also an economically very important gourmet mushroom. because no artificial cultivation is available, natural populations of this speci ...200818302686
traceability of asian matsutake, specialty mushrooms produced by the ectomycorrhizal basidiomycete tricholoma matsutake, on the basis of retroelement-based dna markers.the ectomycorrhizal basidiomycete tricholoma matsutake produces commercially valuable fruit bodies, matsutake, in forests. here we report a pcr system targeting retroelement integration sites to differentiate among individual asian isolates of t. matsutake based on their geographical origins, such as japan, the area of south korea through north korea, the northeastern provinces of china, and the area of the southwestern provinces of china through bhutan. the overall misjudgment rate of the analy ...200818281433
immunostimulatory activities of polysaccharides from liquid culture of pine-mushroom tricholoma matsutake.mushrooms are regarded as one of the well-known foods and biopharmaceutical materials with a great deal of interest. polysaccharide beta-glucan is the major component of mushrooms that displays various biological activities such as antidiabetic, anticancer, and antihyperlipidemic effects. in this study, we compared the immunostimulatory potency of polysaccharide fractions, prepared from liquid culture of pinemushroom tricholoma matsutake, with a potent immunogen lipopolysaccharide (lps), and the ...200818239423
tricholoma matsutake 1-ocen-3-ol and methyl cinnamate repel mycophagous proisotoma minuta (collembola: insecta).two major volatiles produced by the mycelia and fruiting bodies of tricholoma matsutake (1-octen-3-ol and methyl cinnamate) repel a mycophagous collembolan, proisotoma minuta. aggregation of the collembolans on their diet was significantly inhibited by exposure to 1 ppm methyl cinnamate or 10 to 100 ppm 1-octen-3-ol. the aggregation activity decreased dose-dependently upon exposure to 1-octen-3-ol at concentrations higher than 0.01 ppm. aggregation in the presence of methyl cinnamate exhibited t ...200818066606
absorption and tissue distribution of an immunomodulatory alpha-d-glucan after oral administration of tricholoma matsutake.alpha-d-glucan (mpg-1) separated from tricholoma matsutake (cm6271) has been reported to show immunomodulatory activities. in this study, the plasma concentration and tissue distribution of mpg-1 after cm6271 oral administration were investigated as part of the action mechanism analysis. when cm6271 was orally administered in a single dose to mice, mpg-1 was absorbed via the intestinal tract, appeared in plasma after 16 h, was gradually excreted from the blood, and fell to background level after ...200818680305
development of an enzyme-linked immunosorbent assay to detect an immunomodulatory alpha-d-glucan-protein complex, mpg-1, in basidiomycete tricholoma matsutake and related processed foods.we previously isolated a novel immunomodulatory alpha-(1,4)(1,6)(1,2)- d-glucan-protein complex (mpg-1) from mycelia of tricholoma matsutake in basidiomycetes. in the present study, we raised a polyclonal antibody by immunizing rabbits with mpg-1 and constructed a sandwich enzyme-linked immunosorbent assay (elisa) system to examine the distribution of mpg-1 among edible mushrooms and related processed foods. the system detected mpg-1 quantitatively at concentrations of more than 10 ng/ml, with a ...200717867639
single nucleotide polymorphisms in the ectomycorrhizal mushroom tricholoma matsutake.single nucleotide polymorphisms (snps) are becoming increasingly popular markers for studying a variety of biological phenomena. this paper describes the development and analysis of a set of snp markers for the basidiomycete fungus tricholoma matsutake. t. matsutake is a gourmet mycorrhizal mushroom primarily associated with pine forests. however, little is known about its genetics and genomic variation, including snp variation. to identify and analyse snps in t. matsutake, a genomic library was ...200717600046
proliferation of tricholoma matsutake mycelial mats in pine forest using mass liquid inoculum.two isolates of tricholoma matsutake t-008 and t-034, preserved in entomopathogenic fungal culture collection (efcc) of korea, were used in the present study. the isolates had 100% bootstrap homology with tricholoma matsutake u62964 and t. matsutake ab188557 and af309538 preserved in gene bank of ncbi. mycelial growth of t. matsutake was highest in tmm and mya at 25℃. the highest dry wt. of mycelium was obtained after 65 days of culture, when 6 mycelial discs were inoculated in 100 ml of broth i ...200724015070
differentiation of aroma characteristics of pine-mushrooms (tricholoma matsutake sing.) of different grades using gas chromatography-olfactometry and sensory analysis.two independent approaches, gas chromatography-olfactometry and sensory analysis, were used to evaluate and compare the aroma characteristics of pine-mushrooms (tricholoma matsutake sing.) of four different grades. the aroma-active compounds responsible for the sensory attributes of pine- mushrooms were investigated based on the correlation between instrumental and sensory analyses through partial least-square regression. piny, meaty, and floral attributes were strongly correlated with each othe ...200717323969
metabolomic discrimination of different grades of pine-mushroom (tricholoma matsutake sing.) using 1h nmr spectrometry and multivariate data analysis.metabolomic analysis of raw and cooked pine-mushrooms (tricholoma matsutake sing.) of different grades was performed using (1)h nuclear magnetic resonance (nmr) spectrometry and principal component analysis (pca). pca of the (1)h nmr spectra of aqueous fractions allowed different grades of raw pine-mushroom to be discriminated by a combination of principal component (pc) 1 and pc 2, which accounted cumulatively for 94.1% of the variation in all variables. the major peaks in the (1)h nmr spectra ...200717029671
determination of differences in the nonvolatile metabolites of pine-mushrooms (tricholoma matsutake sing.) according to different parts and heating times using 1h nmr and principal component analysis.the differences in the nonvolatile metabolites of pine-mushrooms (tricholoma matsutake sing.) according to different parts and heating times were analyzed by applying principal component analysis (pca) to 1h nuclear magnetic resonance (nmr) spectroscopy data. the 1h nmr spectra and pca enabled the differences of nonvolatile metabolites among mushroom samples to be clearly observed. the two parts of mushrooms could be easily discriminated based on pc 1, and could be separated according to differe ...200718156785
characterization of aroma-active compounds in raw and cooked pine-mushrooms (tricholoma matsutake sing.).the characteristic aroma-active compounds in raw and cooked pine-mushrooms (tricholoma matsutake sing.) were investigated by gas chromatography-olfactometry using aroma extract dilution analysis. 1-octen-3-one (mushroom-like) was the major aroma-active compound in raw pine-mushrooms; this compound had the highest flavor dilution factor, followed by ethyl 2-methylbutyrate (floral and sweet), linalool (citrus-like), methional (boiled potato-like), 3-octanol (mushroom-like and buttery), 1-octen-3-o ...200616910727
difference in the volatile composition of pine-mushrooms (tricholoma matsutake sing.) according to their grades.the differences in volatile components of pine-mushrooms (tricholoma matsutake sing.) according to their grades were observed by applying multivariate statistical methods to gc-ms data sets. a total of 35 and 37 volatile components were identified in raw and cooked pine-mushrooms, respectively. the volatile components in pine-mushrooms were primarily composed of c8 species, such as 3-octanol, 1-octen-3-ol, 1-octanol, (e)-2-octen-1-ol, 3-octanone, 1-octen-3-one, (e)-2-octenal, and octanoic acid. ...200616787034
syntheses and odor descriptions of cyclopropanated compounds, part 6: analogs of aliphatic monoterpene dienols and non-branched alcohols.analogs of aliphatic monoterpene dienols (geraniol, nerol, linalool, and lavandulol) and non-branched alcohols (norleaf alcohol, matsutake alcohol, etc.) bearing a cyclopropane ring were synthesized, and their odor characteristics were examined. most of the analogs show odor properties different from their parent compounds.200617193290
[applicability of gc, gc/ms and liquid chromatography with tandem mass spectrometry to screening for 140 pesticides in agricultural products].the applicability of gc, gc/ms and liquid chromatography with tandem mass spectrometry (lc/ms/ms) to screening for 140 pesticides in agricultural products was examined. validation of multi-residue screening methods for the determination of 88 pesticides in 12 crops (asparagus, cauliflower, burdock, carrot, broccoli, spinach, matsutake mushroom, orange, soybean, sesame, millet and tea) was done by gc and gc/ms. of the 88 pesticides, 63 were obtained with recoveries in the range from 50 to 150% at ...200617128873
a pediatric case of anaphylaxis caused by matsutake mushroom (tricholoma matsutake) ingestion.anaphylaxis is one of the severest forms of allergic diseases. some kinds of mushroom are known as causative allergens in food anaphylaxis. matsutake mushroom (tricholoma matsutake) is a typical edible mushroom available in autumn in japan. we encountered an 8-year-old japanese girl who developed anaphylaxis after ingesting matsutake mushrooms.200617075291
tricholoma matsutake in a natural pinus densiflora forest: correspondence between above- and below-ground genets, association with multiple host trees and alteration of existing ectomycorrhizal communities.tricholoma matsutake (matsutake) is an ectomycorrhizal (ecm) fungus that produces economically important mushrooms in japan. here, we use microsatellite markers to identify genets of matsutake sporocarps and below-ground ecm tips, as well as associated host genotypes of pinus densiflora. we also studied ecm fungal community structure inside, beneath and outside the matsutake fairy rings, using morphological and internal transcribed spacer (its) polymorphism analysis. based on sporocarp samples, ...200616918553
ectomycorrhizal symbiosis in vitro between tricholoma matsutake and pinus densiflora seedlings that resembles naturally occurring 'shiro'.we established an in vitro ectomycorrhizal symbiosis between tricholoma matsutake and pinus densiflora. mycorrhiza formed in a substrate of modified norkrans' c medium and granite-based soil had features similar to those observed previously only in naturally occurring mycorrhizal system called 'shiro,' and promoted the growth of plants with smaller root/shoot ratios. the in vitro formation of 'shiro' is essential for the development of t. matsutake system to produce mushrooms and is useful for t ...200616273392
isolation and characterization of a novel immunomodulatory alpha-glucan-protein complex from the mycelium of tricholoma matsutake in basidiomycetes.tricholoma matsutake, a high-class edible mushroom in japan, has been reported to have excellent biological activities, but difficulty in cultivating the fruit bodies and limited bulk availability have restricted detailed studies. we have developed a method of culturing in tanks, enabling the bulk supply of the mycelia. the preparation (cm6271) exerts modulative effects on the immune competence of mice and rats. in this study, a sodium hydroxide extract of cm6271 was defatted followed by fractio ...200516277387
[antitumor effects of intratumoral injection of basidiomycetes preparations].the antitumor effects of basidiomycetes preparations in an experimental mouse model, the "double grafted tumor system" were analyzed. some brms prevented metastases by utilizing the anti-tumor immunological cascade reactions, which activates macrophages in the body. the following basidiomycetes preparations were analyzed: psk was a hot water extract of cultured mycelia from coliolus versicolor and a protein bound beta-glucan. matsumax was extracted from mycelia of tricholoma matsutake and was a ...200516315899
highly polymorphic dna markers to specify strains of the ectomycorrhizal basidiomycete tricholoma matsutake based on sigmamary1, the long terminal repeat of gypsy-type retroelement mary1.the ectomycorrhizal basidiomycete tricholoma matsutake produces commercially valuable fruit bodies--matsutake--in pinus sp. forest. here we report that pcr with outward facing primers designed based on sequences comprising sigma(mary1), the long terminal repeat of the gypsy-type retroelement mary1, specifies strains of t. matsutake. pcr with a primer based on the 22-bp sequence conserved at the 5'-end of sigma(mary1) conferred 73 reliable bands overall whose profiles depend upon strains of t. ma ...200515375684
genetic mosaics in the massive persisting rhizosphere colony "shiro" of the ectomycorrhizal basidiomycete tricholoma matsutake.the ectomycorrhizal basidiomycete tricholoma matsutake produces commercially valuable fruit bodies "matsutake" on a massive persisting rhizosphere aggregate of mycelia and mycorrhizas called "shiro." using inter-retrotransposon amplified polymorphism analysis, we attempted to explore the potential diversity within the population of t. matsutake isolated from small pinus densiflora woodlands located in various parts of japan. in general, random phylogenetic relationship was noted among t. matsuta ...200515830210
successful inoculation of mature pine with tricholoma matsutake.our finding demonstrates, for the first time, that the roots of mature pine trees can be successfully inoculated with a symbiotic ectomycorrhizal fungus, the valuable matsutake mushroom. long root segments (ca. 5-10 mm in diameter, ca. 50 cm in length) of 50-year-old pinus densiflora trees were excavated, washed, auxin-treated (2-5 mg indole butyric acid, iba, per root) and incubated in moist spagnum moss. twelve months later, short roots were regenerated, of which approximately 90% were free of ...200515856336
stereochemical correlation between 10-hydroperoxyoctadecadienoic acid and 1-octen-3-ol in lentinula edodes and tricholoma matsutake mushrooms.linoleic acid (la) incubated with a homogenate of lentinula edodes or tricholoma matsutake mushroom significantly increased the amount of (r)-1-octen-3-ol. the alcohol was identified as (s)-10-hode with 90-87% and >99% enantiomeric excess (ee), respectively. during the incubation of la with these homogenates in the presence of glutathione-glutathione peroxidase (gsh-gpx), which can reduce hydroperoxy fatty acids to the corresponding hydroxy acids, the formation of (r)-1-octen-3-ol was significan ...200516116283
intra- and inter-specific variations in the copy number of two types of retrotransposons from the ectomycorrhizal basidiomycete tricholoma matsutake.to explore intra- and inter-specific variations of the ectomycorrhizal basidiomycete tricholoma matsutake that produces the fruit body "matsutake", we carried out real-time pcr analysis based on two types of retrotransposons, one designated mary1, which resembles a retrovirus carrying the long terminal repeat (ltr) and the other mary2n, which resembles mrna carrying the polyadenylated tail. calculation based on the average genome size of homobasidiomycetes (34 mbp) shows that ca. 5.5% of the tot ...200515940467
growth promotion of mycelia of the matsutake mushroom tricholoma matsutake by d-isoleucine.mycelial growth of the matsutake mushroom (tricholama matsutake) was much slower than that of the other mushroom species. we found that the addition of d-isoleucine to the culture medium strikingly promoted mycelia growth. the other amino acids tested had no effect on this growth promotion.200415564685
sigma mary1, the ltr of the gypsy-type retroelement mary1 from the basidiomycete tricholoma matsutake, allows multicopy dna integration in lentinula edodes.sigma mary1 is the ltr of the retroelement mary1 from the homobasidiomycete tricholoma matsutake. upon integration through transformation, plc1-hph carrying a sigma mary1 derivative, sigma* mary1, conferred the hygromycin-resistant phenotype stronger than the vector without sigma* mary1 on lentinula edodes. based on the densitometric analysis after southern hybridization, a copy number of the former construct integrated in the genome is much higher than that of the latter. we conclude that sigma ...200414745187
Refresh
firstprev
12
nextlast
Displaying items 1 - 100 of 127