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influence of ephestia kuehniella stage larvae on the potency of bacillus thuringiensis cry1aa delta-endotoxin.the economically important crop pest ephestia kuehniella was tested at two stages of larval development for susceptibility to bacillus thuringiensis cry1aa toxin. bioassays showed that toxicity decreased during the development of larvae stage. in fact, cry1aa toxins from bns3-cry(-) (pht-cry1aa) showed low toxicity against the first-instar larvae (l1) with a lc50 value of about 421.02μg/g of diet and was not toxic against the fifth-instar (l5), comparing to the blb1 toxins used as positive contr ...201728364809
vegetative insecticidal protein of bacillus thuringiensis blb459 and its efficiency against lepidoptera.bacillus thuringiensis strain blb459 supernatant showed a promising activity against lepidopteran pests with extremely damages in the larvae midgut. investigations of the genes that encode secreted toxin demonstrated that this strain harbored a vip3-type gene named vip3(459). based on its original nucleotide and amino acid sequences, this gene was cloned into pet-14b vector and overexpressed in escherichia coli. the expressed protein was purified and tested against different insects and interest ...201728223048
ephestia kuehniella tolerance to bacillus thuringiensis cry1aa is associated with reduced oligomer formation.the basis of the different susceptibility of ephestia kuehniella to the cry1aa and cry1ac δ-endotoxins from bacillus thuringiensis kurstaki bns3 was studied. both toxins bound specifically to the bbmv of e. kuehniella. the result of the ligand blot showed that cry1ac bound to three putative receptors of about 100, 65 and 80 kda and cry1aa interacted only with a 100 kda protein. pronase digestion of the bbmv-bound toxins was used to analyze the toxin insertion. both toxins inserted into the bbmv ...201727888109
isolation and characterization of a new bacillus thuringiensis strain with a promising toxicity against lepidopteran pests.insecticides derived from bacillus thuringiensis are gaining worldwide importance as environmentally desirable alternatives to chemicals for the control of pests in public health and agriculture. isolation and characterization of new strains with higher and broader spectrum of activity is an ever growing field. in the present work, a novel tunisian b. thuringiensis isolate named blb459 was characterized and electrophoresis assay showed that among a collection of 200 b. thuringiensis strains, the ...201727242138
expression of cry1ab gene from a novel bacillus thuringiensis strain sy49-1 active on pest insects.in this study, the cry1ab gene of previously characterized and lepidoptera-, diptera-, and coleoptera-active bacillus thuringiensis sy49-1 strain was cloned, expressed and individually tested on ephestia kuehniella (lepidoptera: pyralidae) and plodia interpunctella (lepidoptera: pyralidae) larvae. pet-cry1ab plasmids were constructed by ligating the cry1ab into pet28a (+) expression vector. constructed plasmids were transferred to an escherichia coli bl21 (de3) strain rendered competent with cac ...201727143037
herbicide toxicity, selectivity and hormesis of nicosulfuron on 10 trichogrammatidae (hymenoptera) species parasitizing anagasta ( = ephestia) kuehniella (lepidoptera: pyralidae) eggs.selective agrochemicals including herbicides that do not affect non-target organisms such as natural enemies are important in the integrated pest management (ipm) programs. the aim of this study was to evaluate the herbicide toxicity, selectivity and hormesis of nicosulfuron, recommended for the corn zea mays l. (poaceae) crop, on 10 trichogrammatidae (hymenoptera) species. a female of each trichogramma spp. or trichogrammatoidea annulata de santis, 1972 was individually placed in plastic test t ...201727726483
relative toxicity of two aphicides to hippodamia convergens (coleoptera: coccinellidae): implications for integrated management of sugarcane aphid, melanaphis sacchari (hemiptera: aphididae).flupyradifurone and sulfoxaflor present novel insecticide chemistries with particular efficacy against aphids, and the recent emergence of sugarcane aphid, melanaphis sacchari (zehntner), as a pest of sorghum in the united states has resulted in their widespread use. we examined their toxicity to hippodamia convergens guerin-meneville, an important aphid biocontrol agent. we exposed beetles to topical applications of the field rate (fr) of these insecticides, fed them contaminated food (eggs of ...201728039423
rearing chrysoperla externa larvae on artificial diets.we tested three artificial diets for rearing larvae of chrysoperla externa (hagen) (neuroptera: chrysopidae), aiming at reducing the production costs of this predator. two of the diets come from studies with other species of lacewings, and the third is a modification described in this paper. all diets were based on animal protein and were supplied to 2nd and 3rd instar larvae, whereas 1st instar larvae received eggs of anagasta kuehniella (zeller) (lepidoptera: pyralidae). we evaluated the preim ...201727530223
an artificial diet containing plant pollen for the mealybug predator cryptolaemus montrouzieri.the specialist predatory ladybird cryptolaemus montrouzieri is an effective natural enemy of mealybugs and plays a key role in the biological control of these pests. however, its mass production is complicated by the dependence on parallel cultures of mealybugs or the need for ephestia kuehniella eggs as an expensive factitious prey.201727146580
impact of factitious foods and prey on the oviposition of the predatory mites gaeolaelaps aculeifer and stratiolaelaps scimitus (acari: laelapidae).the soil-dwelling predatory mites gaeolaelaps aculeifer and stratiolaelaps scimitus (mesostigmata: laelapidae) are important biocontrol agents of several pests (astigmata, thysanoptera, diptera). there is little information regarding the use of factitious foods that potentially improve their mass rearing and population development once released in the field. here we tested the suitability of several types of factitious food and prey for g. aculeifer and s. scimitus. factitious foods included egg ...201627388446
extending the "ecology of fear" beyond prey: reciprocal nonconsumptive effects among competing aphid predators.nonconsumptive effects of predators on prey are well known, but similar effects among competing predators are not. aphidophagous insect larvae are notorious for cannibalism and intraguild predation, as they compete for aggregated but ephemeral prey. we tested for indirect effects of competitors on the development of coleomegilla maculata degeer and hippodamia convergens guerin-meneville (coleoptera: coccinellidae), and a green lacewing, chrysoperla carnea stephens (neuroptera: chrysopidae), with ...201627686817
oviposition model of ephestia kuehniella (lepidoptera: pyralidae).in this study, we examined the fecundity, survival, and adult longevity of ephestia kuehniella (zeller) at different temperatures (15, 17.5, 20, 22.5, 25, 27.5, and 30 °c), and developed a model of oviposition. the fecundity, survival, and adult longevity of e. kuehniella were affected by temperature. longevity reduced from 15.7 d at 15 °c to 8.79 d at 30 °c. total fecundity was lowest (160.54 eggs) at 30 °c and highest (351.75 eggs) at 25 °c. the relationship between temperature and total fecun ...201627567220
can the parasitoid necremnus tutae (hymenoptera: eulophidae) improve existing biological control of the tomato leafminer tuta aboluta (lepidoptera: gelechiidae)?necremnus tutae is native to the mediterranean region where it has been observed in greenhouses parasitizing the invasive tuta absoluta on tomato. the objective of the present study was to determine whether augmentative releases of n. tutae can improve existing biological control of t. absoluta based on predation by nesidicoris tenuis. two experiments were carried out, of which the first evaluated different n. tutae release rates (1 and 2 n. tutae m-2 week-1). the parasitoid reduced plant and fr ...201627121181
extending the "ecology of fear" beyond prey: reciprocal nonconsumptive effects among competing aphid predators.nonconsumptive effects of predators on prey are well known, but similar effects among competing predators are not. aphidophagous insect larvae are notorious for cannibalism and intraguild predation, as they compete for aggregated but ephemeral prey. we tested for indirect effects of competitors on the development of coleomegilla maculata degeer and hippodamia convergens guerin-meneville (coleoptera: coccinellidae), and a green lacewing, chrysoperla carnea stephens (neuroptera: chrysopidae), with ...201628028086
biological activity of two new pyrrole derivatives against stored-product species: influence of temperature and relative humidity.members of the pyrrole group are likely to have interesting properties that merit additional investigation as insecticides at the post-harvest stages of agricultural commodities. in the present work, the insecticidal effect of two new pyrrole derivatives, ethyl 3-(benzylthio)-4,6-dioxo-5-phenyl-2,4,5,6-tetrahydropyrrolo[3,4-c]pyrrole-carboxylate (3i) and isopropyl 3-(benzylthio)-4,6-dioxo-5-phenyl-2,4,5,6-tetrahydropyrrolo[3,4-c]pyrrole-carboxylate (3k) were studied as stored-wheat protectants a ...201627087458
adaptation of habrobracon hebetor (hymenoptera: braconidae) to rearing on ephestia kuehniella (lepidoptera: pyralidae) and helicoverpa armigera (lepidoptera: noctuidae).food characteristics strongly regulate digestive enzymatic activity of insects through direct influences on their midgut mechanisms. insect performance is better on diets that contain nutrients in proportions that fit its digestive enzymes. little is known about the influences of rearing history on parasitism success of habrobracon hebetor say. this research focused on the effect of nutrient regulation on survival, development, and parasitism of h. hebetor. life history and digestive enzyme acti ...201626839317
selectivity of neem to trichogramma pretiosum riley and trichogrammatoidea annulata de santis (hymenoptera: trichogrammatidae).trichogramma pretiosum riley and trichogrammatoidea annulata de santis are commonly found in avocado and persimmon orchards in northern parana state. however, their abundance depends on whether insecticides are used or not to control the key lepidopteran pests stenoma catenifer (wals.) (lepidoptera: elachistidae) and hypocala andremona (stoll) (lepidoptera: noctuidae), respectively. the aim of this work was to evaluate the effects of an aqueous neem seed extract (anse) at 15, 3 and 1.5%, and of ...201621271068
effects of kaolin on lobesia botrana (lepidoptera: tortricidae) and its compatibility with the natural enemy, trichogramma cacoeciae (hymenoptera: trichogrammatidae).lobesia botrana (denis and schiffermüller) (lepidoptera: tortricidae) is an important grapevine pest in europe recently encountered in america. trichogramma cacoeciae marchal (hymenoptera: trichogrammatidae) is amongst the most effective parasitoids for lepidopteran species. studies to evaluate the effect of kaolin, an inert, nontoxic mineral, on oviposition, egg hatch, and neonate mortality of these species were carried out. efficacy on l. botrana neonate larvae, oviposition, and egg hatch was ...201626803817
laboratory performance predicts the success of field releases in inbred lines of the egg parasitoid trichogramma pretiosum (hymenoptera: trichogrammatidae).in this study we assessed the relationship between the laboratory and field performance of different isofemale lines of trichogramma pretiosum riley. in comparative assays, we used three rare mitochondrial haplotypes as genetic markers of the isofemale lines, and by introgressing these mitochondrial haplotypes into each of 15 genetically different nuclear lines, also tested the assumption that mitochondria are neutral markers. in a laboratory trial, 45 isofemale lines (15 nuclear genotypes x thr ...201626730735
two-spotted ladybeetle adalia bipunctata l. (coleoptera: coccinellidae): a commercially available predator to control asian citrus psyllid diaphorina citri (hemiptera: liviidae).the asian citrus psyllid diaphorina citri kuwayama (hemiptera: liviidae) is an economically important pest of citrus because it serves as a vector of the causal pathogens of huanglongbing (hlb) also known as citrus greening disease. the increased use of insecticides for control of d. citri negatively impacts several natural enemies including some effective ladybeetle species which are not available commercially. the two-spotted ladybeetle, adalia bipunctata (coleoptera: coccinellidae) is found i ...201627631730
a promising hd133-like strain of bacillus thuringiensis with dual efficiency to the two lepidopteran pests: spodoptera littoralis (noctuidae) and ephestia kuehniella (pyralidae).isolation and identification of new strains with wide variety of target pests is an ever growing field. in this paper, a screening of 260 strains from tunisian soil samples was conducted by dot-blot and pcr-sequencing analysis. the screening was done on the basis of the possession of cry1d-type genes and was followed by the evaluation of the insecticidal activity against spodoptera littoralis. blb250 strain showed an lc50 value (56.2 μg g(-1)) against s. littoralis lower than those of the two ba ...201627130040
effect of adding amino acids residues in n- and c-terminus of vip3aa16 (l121i) toxin.to study the importance of n- and c-terminus of bacillus thuringiensis vip3aa16 (l121i) toxin (88 kda), a number of mutants were generated. the addition of two (2r: rs) or eleven (11r: rsrpghhhhhh) amino acid residues at the vip3aa16 (l121i) c-terminus allowed to an unappropriated folding illustrated by the abundant presence of the 62 kda proteolytic form. the produced vip3aa16 (l121i) full length form was less detected when increasing the number of amino acids residues in the c-terminus. bioass ...201626876111
cry1ac toxicity enhancement towards lepidopteran pest ephestia kuehniella through its protection against excessive proteolysis.bacillus thuringiensis has been extensively used in agroecosystems for four decades due to its high specific toxicity. strategies based on b. thuringiensis proteins combinations for the improvement of its activity present an important focus for biopesticides development. however, the widespread use of b. thuringiensis δ-endotoxins has often been challenged by a lack of understanding of the target insect physiology as well as its midgut biochemistry. in the present investigation, we have evidence ...201627452929
molecular characterisation of bacillus thuringiensis strain meb4 highly toxic to the mediterranean flour moth ephestia kuehniella zeller (lepidoptera: pyralidae).cry2 proteins play an essential role in current bacillus thuringiensis (bt) applications and in the prevention of insect resistance to cry1a toxins. this paper reports on the screening and characterisation of novel bt strains harbouring effective cry2a-type genes and higher insecticidal activity to ephestia kuehniella.201626103535
life-history traits of macrolophus pygmaeus with different prey foods.macrolophus pygmaeus rambur (hemiptera: miridae) is a generalist predatory mirid widely used in augmentative biological control of various insect pests in greenhouse tomato production in europe, including the invasive tomato leafminer, tuta absoluta (meyrick) (lepidoptera, gelechiidae). however, its biocontrol efficacy often relies on the presence of alternative prey. the present study aimed at evaluating the effect of various prey foods (ephestia kuehniella eggs, bemisia tabaci nymphs, tuta abs ...201627870857
toxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of bacillus thuringiensis crystal protein cry2aa.bacillus thuringiensis δ-endotoxins are the most widely used biopesticides for controlling economically important crop pests and disease vectors. improving their efficacy is of great benefit. here, an improvement in cry2aa δ-endotoxin toxicity was attempted via a cry gene over expression system using p20 from b. thuringiensis israelensis.201626910489
[detection of precipitating antibodies in allergy to ephestia kuehniella]. 20165126424
feeding history affects intraguild interactions between harmonia axyridis (coleoptera: coccinellidae) and episyrphus balteatus (diptera: syrphidae).while the effect of several factors such as predator and prey size, morphology and developmental stage on intraguild predation (igp) is widely investigated, little is known about the influence of diet on the occurrence and outcome of igp. in the present study, the effect of the diet experienced during larval development on igp between the ladybird harmonia axyridis and the syrphid episyrphus balteatus is investigated. four diets were tested for h. axyridis: eggs of the mediterranean flour moth e ...201526030267
development and reproduction of chrysoperla externa (hagen) (neuroptera: chrysopidae) fed on myzus persicae (sulzer) (hemiptera: aphididae) vectoring potato leafroll virus (plrv).the aim of this study was to evaluate the biological parameters of chrysoperla externa (hagen) while feeding on myzus persicae (sulzer) acting as a vector of potato leafroll virus (plrv). in laboratory experiments, three different diets were offered ad libitum to c. externa during the larval period: m. persicae fed on plrv-infected potato leaves, m. persicae fed on uninfected potato leaves, and eggs of the factitious prey anagasta kuehniella (zeller) as the control. the following parameters were ...201526395997
impact of q139r substitution of meb4-cry2aa toxin on its stability, accessibility and toxicity against ephestia kuehniella.the bacillus thuringiensis subsp. kurstaki strain meb4 was previously found to be highly toxic to ephestia kuehniella. sds-page analysis of the recombinant strain dh5α (pbs-cry2aa-meb4) showed that cry2aa-meb4 delta-endotoxins were forming inclusion bodies, and were 2.75 fold more toxic towards e. kuehniella than those of cry2aa-bns3. besides to the 65kda active toxin, proteolysis activation of cry2aa-bns3 protein with e. kuehniella midgut juice generated an extra proteolysis form of 49kda, whic ...201526321422
bowman-birk proteinase inhibitor from clitoria fairchildiana seeds: isolation, biochemical properties and insecticidal potential.herein described is the biochemical characterisation, including in vitro and in vivo assays, for a proteinase inhibitor purified from clitoria fairchildiana seeds (cfpi). purification was performed by hydrophobic interaction and gel filtration chromatography. kinetic studies of the purified inhibitor showed a competitive-type inhibitory activity against bovine trypsin and chymotrypsin, with an inhibition stoichiometry of 1:1 for both enzymes. the inhibition constants against trypsin and chymotry ...201526330217
effects of sublethal concentrations of insecticides on the functional response of two mirid generalist predators.the use of agrochemicals particularly pesticides, can hamper the effectiveness of natural enemies, causing disruption in the ecosystem service of biological control. in the current study, the effects of the insecticides thiacloprid and chlorantraniliprole on the functional response curves were assessed for two mirid predator nymphs, macrolophus pygmaeus rambur and nesidiocoris tenuis reuter. in the absence of insecticides, both predators exhibited a type ii functional response when feeding on eg ...201526641652
eggs of ephestia kuehniella and ceratitis capitata, and motile stages of the astigmatid mites tyrophagus putrescentiae and carpoglyphus lactis as factitious foods for orius spp.several factitious foods were assessed for rearing the anthocorid predators orius thripoborus (hesse) and orius naivashae (poppius) (hemiptera: anthocoridae) in the laboratory. developmental and reproductive traits of both orius species were examined when offered frozen eggs of the mediterranean flour moth, ephestia kuehniella zeller, frozen processed eggs of the medfly, ceratitis capitata wiedemann, or mixed motile stages of the astigmatid mites tyrophagus putrescentiae (schrank) or carpoglyphu ...201526573742
lethal and demographic impact of chlorpyrifos and spinosad on the ectoparasitoid habrobracon hebetor (say) (hymenoptera: braconidae).the appropriate use of biological agents and chemical compounds is necessary to establish successful integrated pest management (ipm) programs. thus, the off-target effects of pesticides on biological control agents are essential considerations of ipm. in this study, the effects of lethal and sublethal concentrations of chlorpyrifos and spinosad on the demographic parameters of habrobracon hebetor (say) (hymenoptera: braconidae) were assessed. bioassays were carried out on immature and adult sta ...201526280986
entomotoxic properties of dioclea violacea lectin and its effects on digestive enzymes of anagasta kuehniella (lepidoptera).entomotoxic plant lectins have been extensively studied in the past two decades, yet the exact mechanisms underlying their toxic effects remain unknown. this study investigated the effects of dioclea violacea lectin (dvl) on larval development in anagasta kuehniella. chronic exposure of larvae (from neonates to the fourth instar) demonstrated that dvl interfered with larval growth, retarding development and decreasing larval mass without affecting survival. dvl decreased trypsin-like, chymotryps ...201526166677
expression of clock genes period and timeless in the central nervous system of the mediterranean flour moth, ephestia kuehniella.homologous circadian genes are found in all insect clocks, but their contribution to species-specific circadian timing systems differs. the aim of this study was to extend research within lepidoptera to gain a better understanding of the molecular mechanism underlying circadian clock plasticity and evolution. the mediterranean flour moth, ephestia kuehniella (pyralidae), represents a phylogenetically ancestral lepidopteran species. we have identified circadian rhythms in egg hatching, adult emer ...201525637625
indigenous aphid predators show high levels of preadaptation to a novel prey, melanaphis sacchari (hemiptera: aphididae).the performance of four aphid predators, hippodamia convergens guerin-meneville, coleomegilla maculata degeer, chrysoperla carnea stephens and orius insidiosus say was compared on three prey species: schizaphis graminum rondani, melanaphis sacchari (zehntner), and ephestia kuehniella zeller eggs. species predatory in both life stages (all except ch. carnea) were reared on e. kuehniella eggs and switched to aphid prey for assessment of reproduction. differences were greater between the e. kuehnie ...201526470381
encapsulated essential oils as an alternative to insecticides in funnel traps.pheromone-lured funnel traps are widely used for pest monitoring and mass trapping in agricultural fields and stores. ddvp vapona (dichlorvos), the insecticide of choice as a killing agent inside traps, has been banned, and research on new products is being pursued. essential oils (eo) could be an alternative if properly formulated. to test their potential, beads of encapsulated coriander and basil eo were tested in funnel traps in stores of almonds and pet foods during 2 mo. the number of adult ...201526470359
egg cannibalism and its life history consequences vary with life stage, sex, and reproductive status in hippodamia convergens (coleoptera: coccinellidae).egg cannibalism is common in coccinellidae, but its biological consequences have not been fully explored. we examined egg cannibalism by neonates, fourth instars, and adults of hippodamia convergens guerin-meneville for effects on development, reproduction, and progeny fitness. we also tested female adults for ability to avoid cannibalizing their own eggs and first-instar larvae, and both sexes for changes in cannibalism propensity following mating, all in the presence of ad libitum food [larvae ...201526470307
fumigant toxicity of essential oils from basil and spearmint against two major pyralid pests of stored products.the fumigant activity of essential oil vapors distilled from sweet basil ocimum basilicum l. and spearmint mentha spicata l. (lamiaceae) were tested against two major stored products pests ephestia kuehniella (zeller) and plodia interpunctella (hübner) (lepidoptera: pyralidae). various oil doses (0.5, 2.5, 5, 50, 250, 500, 1,000, and 1,500 µl/liter air), for an exposure period of 24 h, were tested. the essential oils were subjected to gas chromatography-mass spectrometry analysis and revealed th ...201526470193
sublethal effects of insecticide seed treatments on two nearctic lady beetles (coleoptera: coccinellidae).predatory insects often feed on plants or use plant products to supplement their diet, creating a potential route of exposure to systemic insecticides used as seed treatments. this study examined whether chlorantraniliprole or thiamethoxam might negatively impact coleomegilla maculata and hippodamia convergens when the beetles consumed the extrafloral nectar of sunflowers grown from treated seed. we reared both species on eggs of ephestia kuehniella and then switched adult h. convergens to a die ...201525902962
purification and characterization of a kunitz inhibitor from poincianella pyramidalis with insecticide activity against the mediterranean flour moth.this paper describes the characterization of a trypsin inhibitor from poincianella pyramidalis seeds (ppyti). the partial sequencing of ppyti revealed homology to kunitz inhibitors, clustered as a member of family i03 in merops database. ppyti has a single polypeptide chain of 19,042 da and presents stability at high temperatures (up to 70 °c) and a wide range of ph. in vitro assays showed that disulfide bridges have an important stabilization role of reactive site in ppyti, a characteristic sha ...201525752423
host switching in a generalist parasitoid: contrasting transient and transgenerational costs associated with novel and original host species.parasitoids face challenges by switching between host species that influence survival and fitness, determine their role in structuring communities, influence species invasions, and affect their importance as biocontrol agents. in the generalist parasitoid, venturia canescens (gravenhorst) (hymenoptera: ichneumonidae), we investigated the costs in encapsulation, survival, and body size on juveniles when adult parasitoids switched from their original host, plodia interpunctella (hübner) (lepidoter ...201525691971
supplemental food that supports both predator and pest: a risk for biological control?supplemental food sources to support natural enemies in crops are increasingly being tested and used. this is particularly interesting for generalist predators that can reproduce on these food sources. however, a potential risk for pest control could occur when herbivores also benefit from supplemental food sources. in order to optimize biological control, it may be important to select food sources that support predator populations more than herbivore populations. in this study we evaluated the ...201525349063
purification and characterization of a phenoloxidase in the hemocytes of ephestia kuehniella zeller (lepidoptera: pyralidae): effects of insect growth regulators and endogenous inhibitors.a phenoloxidase was extracted and purified from hemocytes of ephestia kuehniella by using ammonium sulfate, sepharyl g-100 and deae-cellulose fast flow chromatographies. at the final stage of purification, a protein was purified by molecular mass of 78.5 kda, specific activity of 1.17 u/mg protein, recovery of 20.48% and purification fold of 16.71. the purified po showed the highest activity at ph 4-5 and temperatures of 35-40 °c. na(+), k(+), mn(+), zn(2+) and mg(2+) decreased activity of the p ...201525373499
evaluation of various types of supplemental food for two species of predatory mites, amblyseius swirskii and neoseiulus cucumeris (acari: phytoseiidae).although phytoseiids are best known as predators of phytophagous mites and other small arthropods, several species can also feed and reproduce on pollen. in laboratory assays, we assessed the profitability of two types of dietary supplements (three pollen species-cattail, maize and apple-and eggs of the mediterranean flour moth, ephestia kuehniella) for the two species of predatory mites most commonly used as biocontrol agents in horticulture in canada, neoseiulus cucumeris and amblyseius swirsk ...201525430552
flirtation reduces males' fecundity but not longevity.theory predicts that due to limited resources males should strategically adjust their investment in reproduction and survival. based on different conceptual framework, experimental designs, and study species, many studies support while others contradict this general prediction. using a moth ephestia kuehniella whose adults do not feed and thus have fixed resources for their lifetime fitness, we investigated whether males adjusted their investment in various life activities under dynamic socio-se ...201526133013
impact of fluctuating temperatures on development of the koinobiont endoparasitoid venturia canescens.the effect of temperature on the biology of venturia canescens (gravenhorst) (hymenoptera: ichneumonidae) is well understood under constant temperature conditions, but less so under more natural, fluctuating conditions. herein we studied the influence of fluctuating temperatures on biological parameters of v. canescens. parasitized fifth-instar larvae of ephestia kuehniella zeller (lepidoptera: pyralidae) were reared individually in incubators at six fluctuating temperature regimes (15-19.5°c wi ...201525965020
is tetranychus urticae suitable prey for development and reproduction of naïve coleomegilla maculata?the lady beetle coleomegilla maculata de geer is an omnivorous predator that could help suppress aphid and spider mite populations on plants in greenhouses, plantscapes or interiorscapes. we are assessing the nutritional requirements and feeding behavior of c. maculata on target prey (spider mites) and factitious (unnatural) food. our ultimate goal is to develop an efficacious diet to mass produce c. maculata. in this study, we tested the hypothesis that tetranychus urticae koch (two-spotted spi ...201423955814
the trypsin inhibitor from entada acaciifolia seeds affects negatively the development of mediterranean flour moth, anagasta kuehniella.the mediterranean flour moth (anagasta kuehniella) is a pest insect that attacks stored foods. the difficulty in controlling this kind of pest promotes the development of alternatives for pest control, among them the use of proteins with insecticide effect. in this work, we evaluated the role of a trypsin inhibitor purified from entada acaciifolia seeds (eati) on the a. kuehniella development. different concentrations of inhibitor were added to a diet to determine its effects on insect performan ...201424485318
ejaculate economics: an experimental test in a moth.increasing evidence shows that spermatogenesis is costly. as a consequence, males should optimize the use of their sperm to maximize their reproductive outputs in their lifetime. however, experimental evidence on this prediction is largely lacking. here, we examine how a male moth ephestia kuehniella zeller (lepidoptera: pyralidae) responds to the presence of rivals or additional mates and how such response influences his lifetime reproductive fitness. we show that when rival males are present a ...201424429687
a case of ecological specialization in ladybirds: iberorhyzobius rondensis (coleoptera: coccinellidae), potential biocontrol agent of matsucoccus feytaudi (hemiptera: matsucoccidae).specialization is an important attribute of a biological control agent. the maritime pine bast scale, matsucoccus feytaudi ducasse (hemiptera matsucoccidae), is an invasive species in southeast france and the north of italy. iberorhyzobius rondensis eizaguirre (coleoptera: coccinellidae), is a recently described ladybird species. both adults and larvae are predaceous, feeding on egg masses of m. feytaudi, and are strongly attracted to m. feytaudi's sex pheromone. to evaluate the potential of i. ...201424666751
occupational allergy to ephestia kuehniella in the biological control industry. 201425668905
development of chrysoperla carnea (stephens) (neuroptera: chrysopidae) on pollen from bt-transgenic and conventional maize.maize (zea mays) pollen is highly nutritious and can be used by predatory arthropods to supplement or replace a carnivorous diet. we demonstrate that maize pollen can be utilized by larvae of the green lacewing, chrysoperla carnea (neuroptera: chrysopidae) under laboratory conditions. complete development on maize pollen was not possible, but 25% of neonates reached the third instar. when only one instar was fed with pollen and the other two instars with eggs of ephestia kuehniella (lepidoptera: ...201425082074
isolation and characterization of a new bacillus thuringiensis strain lip harboring a new cry1aa gene highly toxic to ephestia kuehniella (lepidoptera: pyralidae) larvae.the aim of this study was to characterize new bacillus thuringiensis strains that have a potent insecticidal activity against ephestia kuehniella larvae. strains harboring cry1a genes were tested for their toxicity, and the lip strain showed a higher insecticidal activity compared to that of the reference strain hd1 (lc50 of lip and hd1 were 33.27 and 128.61 μg toxin/g semolina, respectively). b. thuringiensis lip harbors and expresses cry1aa, cry1ab, cry1ac, cry1ad and cry2a. dna sequencing rev ...201424715255
artificial and factitious foods support the development and reproduction of the predatory mite amblyseius swirskii.the generalist predatory mite amblyseius swirskii athias-henriot (acari: phytoseiidae) was reared on ephestia kuehniella zeller eggs (lepidoptera: pyralidae), decapsulated dry cysts of the brine shrimp artemia franciscana kellogg (anostraca: artemiidae), and on meridic artificial diets (composed of honey, sucrose, tryptone, yeast extract, and egg yolk) supplemented with pupal hemolymph of the chinese oak silkworm antheraea pernyi (guérin-méneville) (lepidoptera: saturniidae) (ad1), with e. kuehn ...201424154947
new bacillus thuringiensis toxin combinations for biological control of lepidopteran larvae.cyt1aa from bacillus thuringiensis israelensis is known by its synergistical activity with b. thuringiensis and bacillus sphaericus toxins. it is able to improve dipteran specific toxins activity and can prevent or overcome larval resistance to those proteins. the objective of the current study was to investigate the possible improvement of larvicidal activity of b. thuringiensis kurstaki expressing heterogeneous proteins cyt1a and p20. cyt1a98 and p20 genes encoding the cytolytic protein (cyt1a ...201424444881
efficacy of olive mill wastewater for protecting bacillus thuringiensis formulation from uv radiations.the effectiveness of 10 low-cost uv-absorbers in protecting bacillus thuringiensis subsp. kurstaki blb1 toxins against inactivation by uv-a and uv-b irradiation was evaluated in this study. among them, two by-products, molasses and olive mill wastewater (omw) were selected for further studies. they were tested at different concentrations of 0.05, 0.1, 0.15 and 0.2% using the para-aminobenzoic acid (paba) as a common uv protectant. interestingly, addition of paba and omw to blb1 formulations was ...201425093915
chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of mediterranean pinus brutia and pinus pinea resin essential oils.essential oils of the resins of pinus brutia and pinus pinea were evaluated for their biological potential. essential oils were characterized using gc-ms and gc/fid. in vitro antimicrobial, phytotoxic, antioxidant, and insecticidal activities were carried out using the direct contact and the fumigant assays, respectively. the chemical profile of the essential oils of the resins of p. pinea and p. brutia included mainly α-pinene (21.39% and 25.40%), β-pinene (9.68% and 9.69%), and caryophyllene ( ...201425556061
factors influencing supercooling capacity of the koinobiont endoparasitoid venturia canescens (hymenoptera: ichneumonidae).venturia canescens is a parthenogenetic koinobiont endoparasitoid of several pyralid moth larvae that are major pests of stored products. low temperatures have been extensively used to control stored-product insects as an alternative method to the application of traditional pesticides. however, most studies have focused on the cold hardiness profile of the major stored-product pests. the objective of this study was to investigate how factors such as age, food, availability of host and acclimatio ...201323913517
response of larval ephestia kuehniella (lepidoptera: pyralidae) to individual bacillus thuringiensis kurstaki toxins mixed with xenorhabdus nematophila.bacillus thuringiensis kurstaki strain bns3 produces parasporal crystals formed by cry1aa, cry1ac and cry2aa delta-endotoxins. in a previous work, we showed that the latter exhibited individually, a weak insecticidal activity against ephestia kuehniella. in order to improve their toxicities, we studied the combined effect of each delta-endotoxin with x. nematophila cells on e. kuehniella larvae growth. xenorhabdus cells were used in combination with spore crystal mixture of the wild strain bns3, ...201323747825
screening and identification of a bacillus thuringiensis strain s1/4 with large and efficient insecticidal activities.the bacterium bacillus thuringiensis was recognized for its entomopathogenic activities related to cry and cyt proteins forming the δ-endotoxins and some extracellular activities like the vegetative insecticidal proteins (vip) and cry1i. these activities may act specifically against diverse organisms and some of them typically characterize each strain. here, we screened a set of 212 b. thuringiensis strains to search the higher insecticidal activities. these strains had bipyramidal and cubic cry ...201322915162
mobility and electric charge of screening pigment granules in the superposition eye of ephestia kuehniella z.the mobility and the electric charge of screening pigment granules of the mealmoth superposition eye were determined electrophoretically in buffer solutions. in potassium phosphate buffer the mobility of the negatively charged granules is linearly dependent on the ph in the range from 4.8 to 7.7, and in veronal buffer from ph = 2.3 to ph = 7.5. at ph = 6.6 the values of the effective charge per granule vary between 9.4 x 10(-17) c and 2.0 x 10(-16) c, those of the real charge between 2.4 x 10(-1 ...20133837106
biological aspects of eriopis connexa (germar) (coleoptera: coccinellidae) fed on different insect pests of maize (zea mays l.) and sorghum [sorghum bicolor l. (moench.)].eriopis connexa (germar) (coleoptera: coccinellidae) occurs in several countries of south america and its mass rearing is important for biological control programmes. this work evaluated biological aspects of e. connexa larva fed on eggs of anagasta kuehniella (zeller) (lepidoptera: pyralidae) and spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae) frozen for one day, fresh eggs of diatraea saccharalis (fabricius) (lepidoptera: pyralidae), s. frugiperda newly-hatched caterpillars, nymph ...201323917572
the effect of rearing in different temperature regimes on the reproduction of anagasta kuehniella (lepidoptera: pyralidae).eggs of anagasta kuehniella (zeller) are widely used in mass rearing of parasitoids, especially trichogramma spp. and predators in many biological-control programs. the objective of this study was to improve the efficiency of mass rearing of a. kuehniella through determining the optimal temperature conditions for rearing, by assessing the effect of temperature during the developmental stages on the reproduction of a. kuehniella. we evaluated 1) the effect of temperature at which a. kuehniella wa ...201323905744
effect of carbon dioxide (co2) on mortality and reproduction of anagasta kuehniella (zeller 1879), in mass rearing, aiming at the production of trichogramma spp.eggs of anagasta kuehniella (zeller 1879) are widely used for mass rearing of trichogramma spp. and other parasitoids and predators, largely commercialized in many countries. the aim of this study is to evaluate the effect of carbon dioxide (co2) originated from larval metabolism on the biological parameters of a. kuehniella. for that purpose, we assess the production of carbon dioxide (co2) per rearing tray of a. kuehniella and the effect of co2 on the viability of egg-to-adult period and ovipo ...201323828359
activity of eight strains of entomopathogenic nematodes (rhabditida: steinernematidae, heterorhabditidae) against five stored product pests.stored product pests are responsible for losses that can amount 10% during cereal storage in the world. aiming to find an alternative method to the chemicals used for the stored-product pests, eight strains of entomopathogenic nematodes (epns) were tested against five species of stored product pests. the bioassays were conducted in microtubes containing paper, inoculated with epns and insect diet. all the insect species were susceptible to the epns strains. anagasta kuehniella and tenebrio molit ...201323567251
insecticidal effect of chlorantraniliprole against major stored-product insect pests in different grain commodities under laboratory tests.chlorantraniliprole belongs to the chemical group of anthranilic diamides. it has selectivity to beneficial arthropods and low mammalian toxicity. laboratory bioassays were conducted to assess chlorantraniliprole as a potential grain protectant against ephestia kuehniella zeller larvae, liposcelis bostrychophila badonnel adults, rhyzopertha dominica (f.) adults, sitophilus oryzae (l.) adults and tribolium confusum jacquelin du val adults and larvae. factors such as dose (0.01, 0.1, 1 and 10 mg c ...201323554236
mating-disruption trials for control of mediterranean flour moth, ephestia kuehniella zeller (lepidoptera: pyralidae), in traditional flour mills.in the present work, 3 years of field trials (from 2007 to 2009) were carried out to evaluate use of the pheromone (9z, 12e)-tetradecadienyl acetate (tda) for mating disruption (md) of the mediterranean flour moth, ephestia kuehniella zeller. dispensers containing the pheromone tda were placed in two traditional flour mills. pheromone-baited funnel traps were used to monitor the population fluctuations of e. kuehniella males throughout the entire experimental period; female oviposition was asses ...201323462083
purification of a kunitz-type inhibitor from acacia polyphylladc seeds: characterization and insecticidal properties against anagasta kuehniella zeller (lepidoptera: pyralidae).anagasta kuehniella is a polyphagous pest that causes economic losses worldwide. this species produces serine proteases as its major enzymes for protein digestion. in this study, a new serine-protease inhibitor was isolated from acacia polyphylla seeds (acki).further analysis revealed that acki is formed by two polypeptide chains with a relative molecular mass of ∼20 kda. the effects of acki on the development, survival, and enzymatic activity of anagasta kuehniella larvae were evaluated, by inc ...201323418748
suitability of the pest-plant system tuta absoluta (lepidoptera: gelechiidae)-tomato for trichogramma (hymenoptera: trichogrammatidae) parasitoids and insights for biological control.the south american tomato leafminer, tuta absoluta meyrick (lepidoptera: gelechiidae), is a major pest that has recently invaded afro-eurasia. biological control, especially by trichogramma parasitoids, is considered to be promising as a management tool for this pest. however, further development of trichogramma-based biocontrol strategies would benefit from assessing the impact of released parasitoid offspring on the pest. under laboratory conditions, we 1) compared the parasitism of five trich ...201324498728
high-throughput sequencing of a single chromosome: a moth w chromosome.y and w chromosomes have mostly been excluded from whole genome sequencing projects. due to the high amount of repetitive sequences they are 'difficult' to assemble and therefore need special treatment in the form of, e.g. adapted assembly programs, a range of different libraries, and accurate maps, if possible. a minimum requirement for these approaches is pure template dna. we therefore microdissected the w chromatin of highly polyploid cells from the flour moth, ephestia kuehniella, and used ...201323949445
inheritance of a temperature-modified phenotype of the short antennae (sa) mutation in a moth, ephestia kuehniella (lepidoptera: pyralidae).the autosomal recessive mutation short antennae (sa) causes considerable shortening of antennae in male and female mediterranean flour moths (ephestia kuehniella zeller). however, the sa phenotype can be suppressed by several physical factors, making sa moths indistinguishable from wild-type moths (sa(wt)). this can be done by subjecting larva and pupa to a higher temperature (25 degrees c), to lithium ions, or to an alternate electric field. the first half of pupal development was found to be t ...201311447238
is the sex communication of two pyralid moths, plodia interpunctella and ephestia kuehniella, under circadian clock regulation?females of the indian meal moth, plodia interpunctella, and females of the mediterranean flour month, ephestia kuehniella (both lepidoptera: pyralidae), exhibit daily rhythms in calling behavior. the peak in p. interpunctella calling occurs at dusk, whereas e. kuehniella calls preferentially at dawn. this behavior turned arrhythmic in p. interpunctella females in constant darkness (dd) and remained arrhythmic in constant light (ll), whereas e. kuehniella females showed a persistent rhythm in dd ...201222653889
insecticidal effect of labramin, a lectin-like protein isolated from seeds of the beach apricot tree, labramia bojeri, on the mediterranean flour moth, ephestia kuehniella.the objective of this work was to study the insecticidal effect of labramin, a protein that shows lectin-like properties. labramin was isolated from seeds of the beach apricot tree, labramia bojeri a. dc ex dubard (ericales: sapotaceae), and assessed against the development of the mediterranean flour moth ephestia kuehniella zeller (lepidoptera: pyralidae), an important pest of stored products such as corn, wheat, rice, and flour. results showed that labramin caused 90% larval mortality when inc ...201222938183
lethal doses of ozone for control of all stages of internal and external feeders in stored products.gaseous ozone (o(3)) has potential for control of insects in stored grain. previous studies have focused on freely exposed insects. immatures of internal pests (e.g. sitophilus spp. and most stages of rhyzopertha dominica f.) are protected within kernels and probably require higher doses and/or longer treatment times for full control. a laboratory study determined the doses of ozone necessary for full control of freely exposed and internal stages of eleven stored-product pest species. test insec ...201222566141
maternal effects shape dynamic trajectories of reproductive allocation in the ladybird coleomegilla maculata.we followed lifetime trajectories of reproductive allocation in coleomegilla maculata females of three different size classes produced by rearing beetles on three different daily larval feeding regimes (30 min, 6 h or ad libitum access to eggs of ephestia kuehniella). we hypothesized that small females would produce fewer and smaller eggs than larger females and that reproductive effort would decline with female age. females were mated with a male from the same treatment and then isolated with a ...201222475542
nectarivory by the plant-tissue feeding predator macrolophus pygmaeus rambur (heteroptera: miridae): nutritional redundancy or nutritional benefit?most predators and parasitoids feed on plant-provided food (nectar, pollen) or engage in herbivory during at least part of their life stages. plant feeding by these insects plays an important role in driving predator-herbivore dynamics. thus, understanding the effects of plant feeding on omnivores could be an important element in improving biological control strategies. the mirid macrolophus pygmaeus is an omnivorous heteropteran predator of whitefly and other pests. unlike other predators that ...201222245441
synthesis method for thiosulfonate and report of its insecticidal activity in anagasta kuehniella (lepidoptera: pyralidae).insect pests have caused economic losses valued at billions of dollars in agricultural production. anagasta kuehniella (zeller), the mediterranean flour moth, is of major economic importance as a flour and grain feeder and is often a severe pest in flourmills. this study provides a suitable route for the direct preparation of thiosulfonates 2 and 3 from thiols, under mild conditions, with good yields; these thiosulfonates were tested for their regulatory effect on insect growth. the chronic inge ...201223203122
organ growth without cell division: somatic polyploidy in a moth, ephestia kuehniella.organ growth depends on cell division and (or) cell growth. here, we present a study on two organs whose growth depends entirely on cell growth, once they are formed in the embryo: malpighian tubules and silk glands of the flour moth, ephestia kuehniella . between first and last larval instar, the volume of malpighian tubule cells increases by a factor of ∼1800 and that of silk gland cells by a factor of ∼3100. we determined the number of endocyles required to reach these stages by feulgen cytom ...201223057509
development of an early-tier laboratory bioassay for assessing the impact of orally-active insecticidal compounds on larvae of coccinella septempunctata (coleoptera: coccinellidae).early-tier studies are the initial step in the environmental risk assessment of genetically engineered plants on nontarget arthropods. they are conducted in the laboratory where surrogate species are exposed to higher concentrations of the arthropod-active compound than those expected to occur in the field. thus, early-tier tests provide robust data and allow to make general conclusions about the susceptibility of the surrogate to the test substance. we have developed an early-tier test for asse ...201223321119
influence of diet on fecundity, immune defense and content of 2-isopropyl-3-methoxypyrazine in harmonia axyridis pallas.food type can affect all functional aspects of an insect's life. we investigated the effects of different diet regimes on life history parameters of the ladybird beetle harmonia axyridis. furthermore, we tested the importance of elytral color, sex, and diet on chemical and immune defense in this species. we also compared hemolymph from cohorts of h. axyridis and coccinella septempunctata (coleoptera: coccinellidae) fed different diets to examine effects on the 2-isopropyl-3-methoxypyrazine (ipmp ...201222648506
moisture source and diet affect development and reproduction of orius thripoborus and orius naivashae, two predatory anthocorids from southern africa.the effect of moisture source and diet on the development and reproduction of the pirate bugs, orius thripoborus (hesse) and orius naivashae (poppius) (hemiptera: anthocoridae) was examined in the laboratory. both species had been collected in and around sugarcane fields in south africa. supplementing eggs of the flour moth ephestia kuehniella (zeller) (lepidoptera: pyralidae) with a green bean pod as a moisture source yielded better nymphal survival and faster development, as compared with free ...201222935002
molecular characterization of ephestia kuehniella (lepidoptera: pyralidae) transferrin and its response to parasitoid venturia canescens (hymenoptera: ichneumonidae gravenhorst).in the present study, we characterized a full-length cdna encoding a putative iron-binding protein transferrin from the lepidopteran mediterranean flour moth (ektrf, 2397 bp). the putative ektrf is 683 amino acids with a molecular mass of approximately 76 kda. the deduced amino acid sequence showed significant homology with other insect transferrins from chilo suppressalis (76%), galleria mellonella (75%), plutella xylostella (72%), manduca sexta (74%), bombyx mori (73%), spodoptera litura and ( ...201222229520
amino acids y229 and f603 are involved in bacillus thuringiensis cry1ac delta-endotoxin stability and toxicity.bacillus thuringiensis cry1ac toxin shares structurally five conserved blocs with the other delta-endotoxins. in order to study the role of some amino acids belonging to these regions, two mutations, y(229) p and f(603) s, have been introduced separately and respectively in blocs 2 and 5. the resulting mutant proteins cry1ac'1 and cry1ac'3 were affected in their stability and crystallization. both of them lost their toxicity to the lepidopteran larvae ephestia kuehniella. unlike cry1ac'1, cry1ac ...201222268917
a trypsin inhibitor from sapindus saponaria l. seeds: purification, characterization, and activity towards pest insect digestive enzyme.the present paper describes the purification, characterization and determination of the partial primary structure of the first trypsin inhibitor isolated from the family sapindaceae. a highly stable, potent trypsin inhibitor (ssti) was purified to homogeneity. sds-page analysis revealed that the protein consists of a two-polypeptide chain with molecular masses of approximately 15 and 3 kda. the purified inhibitor inhibited bovine trypsin at a 1:1 m ratio. kinetic analysis revealed that the prote ...201121127952
overcoming the production limitations of photorhabdus temperata ssp. temperata strain k122 bioinsecticides in low-cost medium.for low-cost production of photorhabdus temperata ssp. temperata strain k122 bioinsecticide, a cheap complex medium was optimized. diluted seawater was used as the source of micronutrients, especially sodium chloride, involved in the improvement of cell density, culturability and oral toxicity of the bacterium p. temperata against ephestia kuehniella larvae. thus, the new formulated medium was composed only of 10 g/l of soya bean meal, used as the carbon and nitrogen main source, mixed in sevenf ...201121656156
insecticide selectivity to two species of trichogramma in three different hosts, as determined by iobc/wprs methodology.background: the use of trichogramma species is a potential key strategy in integrated pest management. however, its effectiveness depends on the use of chemicals that do not interfere with parasitism and parasite population growth. here, a study was made of the effects of synthetic insecticides on trichogramma pretiosum and trichogramma exiguum in different hosts (ephestia kuehniella, plutella xylostella and spodoptera frugiperda) and the influence of international organisation for biological co ...201121858909
three homopteran pests of citrus as prey for the convergent lady beetle: suitability and preference.the convergent lady beetle, hippodamia convergens guérin-méneville (coleoptera: coccinellidae), is an important predator of soft-bodied insect pests in many regions of the united states, but generally uncommon in florida citrus. certain citrus producers in florida recently initiated releases of commercially available h. convergens from california against the asian citrus psyllid diaphorina citri kuwayama, vector of huanglongbing or citrus greening disease. however, there is little information o ...201122217767
integration of a recombinant chitinase into bacillus thuringiensis parasporal insecticidal crystal.chitinases have been successfully used in combination with bacillus thuringiensis delta-endotoxins forming crystals in order to enhance their insecticidal activities. in this context, we opted for promoting the chitinase integration into these crystals. thus, we engineered, for the first time, a fusion protein (cdf) consisting of the chitinase chi255 and the carboxy-terminal half of cry1ac, both from b. thuringiensis subsp. kurstaki. the constructed transcriptional fusion (chi255δsp-ctcry1ac) wa ...201120625731
investigation of the steps involved in the difference of susceptibility of ephestia kuehniella and spodoptera littoralis to the bacillus thuringiensis vip3aa16 toxin.bupm95 is a bacillusthuringiensis subsp. kurstaki strain producing the vip3aa16 toxin with an interesting insecticidal activity against the lepidopteran larvae ephestia kuehniella. study of different steps in the mode of action of this vegetative insecticidal protein on the mediterranean flour moth (e. kuehniella) was carried out in the aim to investigate the origin of the higher susceptibility of this insect to vip3aa16 toxin compared to that of the egyptian cotton leaf worm spodoptera littoral ...201121600212
fumigant toxicity and oviposition deterrency of the essential oil from cardamom, elettaria cardamomum, against three stored–product insects.use of insecticides can have disruptive effects on the environment. replacing the chemical compounds in these insecticides with plant materials, however, can be a safe method with low environmental risk. in the current study, chemical composition and insecticidal activities of the essential oil from cardamom, elettaria cardamomum l. (maton) (zingiberales: zingiberaceae) on the adults of three stored product pests was investigated. results indicated that essential oil of e. cardamomum toxic to th ...201122242564
chemical composition and fumigant toxicity of some essential oils against ephestia kuehniella.the chemical constituents of some essential oils extracted from aromatic plants (savory, satureja thymbra l.; turkish oregano, origanum onites l.; myrtle, myrtus communis l.; marjoram, origanum majorana l.; laurel, laurus nobilis l.; lemon, citrus limon l.; sticky goosefoot, chenopodium botrys l.; and tansy, tanecetum armenum [dc.] suchultz bip.) were analyzed by gas chromatography-mass spectrometry. fumigant toxicity of volatile compounds was tested against mediterranean flour moth, ephestia ku ...201121882685
galactolipase, phospholipase and triacylglycerol lipase activities in the midgut of six species of lepidopteran larvae feeding on different lipid diets.galactolipase, phospholipase and triacylglycerol lipase activities were measured from the midgut of six species of lepidopteran larvae, two folivores, epiphyas postvittana (tortricidae) and helicoverpa armigera (noctuidae); two granivores, plodia interpunctella (pyralidae) and ephestia kuehniella (pyrallidae); a presumptive carnivore, galleria mellonella (pyralidae); and a keratinophage, tineola bisselliella (tineidae). galactolipase has not been previously reported in insects. galactolipase and ...201121704634
effects of food deprivation on the development of coleomegilla maculata (de geer) (coleoptera: coccinellidae).the lady beetle coleomegilla maculata (de geer) is a natural enemy of several insect pests and feeds on pollen and nectar to survive periods when prey is scarce. the effect of the feeding interval on the development, survival, fecundity, and longevity of c. maculata was determined. newly hatched larvae of c. maculata were reared individually and fed with eggs of the mediterranean flour moth anagasta kuehniella (zeller) at intervals of one, two, and three days under controlled conditions (23 ± 1º ...201121437492
seminal fluid reduces female longevity and stimulates egg production and sperm trigger oviposition in a moth.previous studies suggest that a number of factors in relation to mating may reduce female longevity and stimulate egg production and oviposition. however, it is still not clear whether these factors act on these parameters independently or in a collective way. here we carried out a series of experiments including mating trials and seminal fluid injection to determine the factors responsible for reducing female longevity and stimulating egg production and oviposition in relation to mating in the ...201121172356
laboratory studies of blattisocius keegani (fox) (acari: ascidae) reared on eggs of navel orangeworm: potential for biological control.blattisocius keegani (fox) is a predatory mite in the family ascidae (acari), noted for potential biological control of coleopteran stored product pests. performance of b. keegani on eggs of navel orangeworm, amyelois transitella walker (lepidoptera: pyralidae), was investigated. mites completed development from egg to adult in 9.2±0.22 days at 25°c and 50-60% relative humidity, and in 6.33±0.29 days at 32.2°c, 30% relative humidity. mites provisioned with three or five eggs consumed a median of ...201121067628
effect of host change on demographic fitness of the parasitoid, trichogramma brassicae.trichogramma brassicae (bezdenko) is the most important species of trichogramma parasitoids in iran. the cotton bollworm, helicoverpa armigera (hubner) is a polyphagous insect pest that attacks many crops including cotton, maize, soybean, tomato, etc. the bollworm egg is a suitable target for many trichogramma species. factitious hosts such as eggs of the flour moth, anagasta kuehniella (zeller) and cereal moth, sitotroga cerealella (hubner) are used for mass rearing purposes. but a problem that ...201020673197
form and nature of precopulatory sexual selection in both sexes of a moth.sexual selection is a process that operates through intrasexual competition and intersexual choice for reproduction in both sexes. here, we report our work on a polygamous moth, ephestia kuehniella zeller (lepidoptera: pyralidae), aiming to infer the form and nature of precopulatory sexual selection in males and females. we show that, although a number of traits measured in each sex are correlated with mating success, the primary selection trait in females appears to be abdominal thickness and t ...201020473478
occurrence of nosema oryzaephili in cryptolestes ferrugineus and transfer to the genus paranosema.a microsporidium that closely resembles paranosema species at the level of the light microscope was isolated from the rusty grain beetle, cryptolestes ferrugineus. it's identity as nosema oryzaephili (originally described from oryzaephilus surinamensis) was confirmed by comparison with a known isolate of n. oryzaephili based on spore size, small subunit rdna sequence, and relative infectivity to o. surinamensis, tribolium castaneum, and ephestia kuehniella. phylogenetic analysis of the small sub ...201020460127
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