Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
identification of bacteria in blood cultures from clinically ill captive antillean manatees (trichechus manatus manatus).between september 2001 and march 2013, 62 bacterial cultures (37 aerobic and 25 anaerobic) were performed on 37 blood samples from 23 antillean manatees ( trichechus manatus manatus) that were kept in captivity at the brazilian national center for research and conservation of aquatic mammals (cma) in pernambuco (cma-pe) and alagoas (cma-al), brazil. all of the animals sampled exhibited clinical signs at the time of sampling including abscesses (n = 8), debilitation and anorexia (n = 22), and pro ...201728363079
enantioselective resolution of (r,s)-carvedilol to (s)-(-)-carvedilol by biocatalysts.among the microorganisms employed in the study, aspergillus niger (gufcc5443), escherichia coli (atcc9637), streptomyces halstedii (ckm-2), pseudomonas putida (ncib9494), cunninghamella elegans (ncim689) and sphingomonas paucimobilis (nctc11030) were capable for the enantioselective conversion of racemic carvedilol. immobilization technique enhanced the enantioselectivity of microorganisms and thus increased the enantiomeric purity of the drug. excellent enantiomeric ratios (e) were found in rea ...201728064425
coexistence of free-living amoebae and bacteria in selected south african hospital water distribution systems.pathogenic free-living amoebae (fla), such as naegleria fowleri, balamuthia mandrillaris and acanthamoeba species isolated from aquatic environments have been implicated in central nervous system, eye and skin human infections. they also allow the survival, growth and transmission of bacteria such as legionella, mycobacteria and vibrio species in water systems. the purpose of this study was to investigate the co-occurrence of potentially pathogenic fla and their associated bacteria in hospital w ...201727730363
risk of infection transmission in curvilinear array echoendoscopes: results of a prospective reprocessing and culture registry.the complex design of the elevator mechanism in duodenoscopes has been recognized as a challenge for disinfection and recently implicated as a potential source of persistent bacterial contamination. curvilinear array (cla) echoendoscopes also have an elevator mechanism; however, there are no recommendations or data regarding the risk of persistent bacterial contamination of echoendoscopes. here we hoped to determine the yield of microbial growth with routine bacterial surveillance cultures of re ...201727478029
structure of aryl o-demethylase offers molecular insight into a catalytic tyrosine-dependent mechanism.some strains of soil and marine bacteria have evolved intricate metabolic pathways for using environmentally derived aromatics as a carbon source. many of these metabolic pathways go through intermediates such as vanillate, 3-o-methylgallate, and syringate. demethylation of these compounds is essential for downstream aryl modification, ring opening, and subsequent assimilation of these compounds into the tricarboxylic acid (tca) cycle, and, correspondingly, there are a variety of associated aryl ...201728373573
improving water quality in a dialysis unit using root cause analysis.water quality levels from hemodialysis (hd) and reverse osmosis (ro) machines in dialysis units must meet standards set by the american association of medical instrumentation. researchers used a root cause analysis (rca) approach to identify and address factors affecting water quality in the hd and portable ro machines at our institution.201728302433
the autophagy machinery restrains inkt cell activation through cd1d1 internalization.invariant natural killer t (inkt) cells are innate t cells with powerful immune regulatory functions that recognize glycolipid antigens presented by the cd1d protein. while inkt cell-activating glycolipids are currently being explored for their efficacy to improve immunotherapy against infectious diseases and cancer, little is known about the mechanisms that control cd1d antigen presentation and inkt cell activation in vivo. cd1d molecules survey endocytic pathways to bind lipid antigens in mhc ...201728296542
sphingomonas paucimobilis septicemia in a neonate: a rare case report.sphingomonas paucimobilis , a yellow-pigmented, aerobic, glucose nonfermenting, gram-negative bacilli is a rare cause of human infection. it was first discovered as an infective agent in humans in 1977 and named pseudomonas paucimobilis. it was renamed as s. paucimobilis in 1990 in accordance with phylogenetic data. s. paucimobilis is an aerobic bacterium found in soil and water; it is a rare cause of healthcare associated infections. s. paucimobilis can cause infections in healthy as well as im ...201726960657
[spontaneous bacterial peritonitis due to sphingomonas paucimobilis in a cirrhotic patient]. 201726620600
sphingomonas paucimobilis peritonitis: a case report and review of the literature.sphingomonas paucimobilis, a yellow-pigmented, aerobic, glucose non-fermenting, gram-negative bacillus, is a rare cause of human infection normally associated with immunocompromised hosts. it has been associated with a few cases of continuous ambulatory peritoneal dialysis (pd) and is notorious for its resistance to the commonly used antibiotics. in half of the cases reported so far, the peritonitis was refractory to treatment, necessitating pd catheter removal. we report a case of sphingomonas ...201726022030
sphingomonas paucimobilis causing pacemaker pocket infection in a pediatric patient with a hemangioma. 201626781221
frequency of colonization and isolated bacteria from the tip of epidural catheter implanted for postoperative analgesia.the increased use of epidural analgesia with catheter leads to the need to demonstrate the safety of this method and know the incidence of catheter colonization, inserted postoperatively for epidural analgesia, and the bacteria responsible for this colonization.201625925032
presence of bacterial virulence gene homologues in the dibenzo-p-dioxins degrading bacterium sphingomonas wittichii.sphingomonas wittichii, a close relative of the human pathogen sphingomonas paucimobilis, is a microorganism of great interest to the bioremediation community for its ability of biodegradation to a large number of toxic polychlorinated dioxins. in the present study we investigated the presence of different virulence factors and genes in s. wittichii. we utilized phylogenetic, comparative genomics and bioinformatics analysis to investigate the potentiality of s. wittichii as a potential virulent ...201628197061
post-clear corneal phacoemulsification endophthalmitis: profile and management outcomes at a tertiary eye care center in western india.infectious endophthalmitis is a serious sight threatening intraocular inflammation that results from exogenous or endogenous spread of organisms into the eye.a retrospective case series to study the profile of endophthalmitis following clear corneal phacoemulsification in western india between years 2008 and 2014 was held in the national institute of ophthalmology, pune, india. cases of endophthalmitis post-clear corneal phacoemulsification were reviewed pertaining to demography, clinical histor ...201627896783
degradation of lignin β-aryl ether units in arabidopsis thaliana expressing ligd, ligf and ligg from sphingomonas paucimobilis syk-6.lignin is a major polymer in the secondary plant cell wall and composed of hydrophobic interlinked hydroxyphenylpropanoid units. the presence of lignin hampers conversion of plant biomass into biofuels; plants with modified lignin are therefore being investigated for increased digestibility. the bacterium sphingomonas paucimobilis produces lignin-degrading enzymes including ligd, ligf and ligg involved in cleaving the most abundant lignin interunit linkage, the β-aryl ether bond. in this study, ...201627775869
successful return to peritoneal dialysis after a case of relapsing sphingomonas paucimobilis peritonitis
.sphingomonas paucimobilis is an uncommon source of peritonitis in patients undergoing peritoneal dialysis (pd). although only a small number of cases have been reported, treatment failure rate is extremely high, with removal of the peritoneal dialysis catheter noted in ~ 50% of reported cases. the potential damage to the peritoneal membrane from peritonitis with this organism and the ability to return to pd after infection is unknown. we report a unique case in which a patient was able to succes ...201627730806
recurrent complicated urinary tract infection due to rare pathogen sphingomonas paucimobilis: contamination or real deal?sphingomonas paucimobilis is an aerobic, oxidase-positive, yellow-pigmented, non-fermentative, gram-negative opportunistic pathogen that rarely causes infections in humans. it is commonly found in nosocomial environments and, despite its low clinical virulence, it can be responsible for several different infections especially among patients with underlying disease. here we describe a clinical case of a 46-year-old male paraplegic patient with a history of neurogenic bladder due to insulin-depend ...201627668907
oral ulcer by sphingomonas paucimobilis: first report.the first case of an oral infection caused by sphingomonas paucimobilis is reported. a 73-year-old man presented with a gingival ulcer with bone exposure affecting the attached gingiva in the anterior maxillary region. he reported pain during chewing and the presence of fever. since the first case of s. paucimobilis infection was reported in 1977, involving a leg ulcer, the number of reports related to this organism has been increasing, indicating that the bacterium should be considered an emerg ...201627515851
modulation of dendritic-epithelial cell responses against sphingomonas paucimobilis by dietary fibers.non-fermenting gram-negative bacilli, such as sphingomonas paucimobilis (s.paucimobilis), are among the most widespread causes of nosocomial infections. up to now, no definitive guidelines exist for antimicrobial therapy for s. paucimobilis infections. as we have shown that some dietary fibers exhibit pronounced immune-regulatory properties, we hypothesized that specific immune active dietary fibers might modulate the responses against s. paucimobilis. we studied the immunomodulatory effects of ...201627452116
a tetrahydrofolate-dependent methyltransferase catalyzing the demethylation of dicamba in sphingomonas sp. strain ndbn-20.sphingomonas sp. strain ndbn-20 degrades and utilizes the herbicide dicamba as its sole carbon and energy source. in the present study, a tetrahydrofolate (thf)-dependent dicamba methyltransferase gene, dmt, was cloned from the strain, and three other genes, metf, dhc, and puru, which are involved in thf metabolism, were found to be located downstream of dmt a transcriptional study revealed that the four genes constituted one transcriptional unit that was constitutively transcribed. lysates of c ...201627422839
a lethal case of sphingomonas paucimobilis bacteremia in an immunocompromised patient.sphingomonas paucimobilis is a yellow-pigmented, glucose nonfermenting, aerobic, gram negative bacillus of low pathogenicity. this organism was found in the implantation of indwelling catheters, sterile intravenous fluid, or contaminated hospital environment such as tap and distilled water, nebulizer, ventilator, and hemodialysis device. a 55-year-old female was hospitalized for diabetic foot ulcer in the presence of multiple comorbidities: diabetes mellitus, colonic tuberculosis, end-stage rena ...201627088020
kinetics of bisphenol a degradation by sphingomonas paucimobilis fj-4.a chemostat was operated to characterize degradation of bisphenol a by sphingomonas paucimobilis fj-4. the chemostat at 30°c was fed with a medium containing 150 mg l(-1) of bpa as the sole carbon and energy source. at the short cell retention time of 8 h, the bacterial cells were washed out from the chemostat. at long cell retention times of 12, 16, 24, and 48 h, steady-states of the bacterial growth on bpa degradation were achieved after a lag time of 16-57 h. a mathematical model was applied ...201627038671
[prevalence of sensitization to fungi in patients with respiratory allergy].as part of the etiology of respiratory allergy we have genetics, prenatal factors and sensitivity to various airborne allergens, between these fungi are found. relationship has been found between sensitization to fungal in skin tests and allergy pathogenesis and aggravation. there is a few literature in mexico and in the north of the country it is lacking regarding this problem.201627174757
[prevalence of sensitization to allergens in school children with asthma residents from guadalajara metropolitan area].allergic sensitization is dependent on the geographical and climatic conditions in each region; therefore, identify agents most commonly sensitized children with asthma is important for planning prevention measures.201627174756
[identification of microorganisms related to chronic rhinosinusitis in adult patients with variable common immunodeficiency].the prevalence of chronic rhinosinusitis in adult patients with common variable immunodeficiency (cvid) is 52%. the patients with cvid show higher incidence of chronic rhinosinusitis, which is an inflammatory disease that affects the lining of one or more paranasal sinuses and nasal cavity.201626943827
epizootiological characteristics of viable bacteria and fungi in indoor air from porcine, chicken, or bovine husbandry confinement buildings.microorganisms found in bioaerosols from animal confinement buildings not only foster the risk of spreading diseases among livestock buildings, but also pose health hazards to farm workers and nearby residents. this study identified the various microorganisms present in the air of swine, chicken, and cattle farms with different kinds of ventilation conditions in korea. microbial air samples were collected onto petri dishes with bacterial or fungal growth media using a cascade impactor. endotoxin ...201627456779
sphingomonas paucimobilis bacteremia in an hiv and hepatitis c co-infected patient.prompt diagnosis and treatment of blood stream infections in an immunocompromised host can result in a decrease in mortality with appropriate antibiotic coverage. signs, symptoms, physical examination, cultures and sensitivity findings must be evaluated and interpreted together to accurately diagnose and manage the problem.201625841882
non-oral gram-negative facultative rods in chronic periodontitis microbiota.the subgingival prevalence of gram-negative facultative rods not usually inhabiting or indigenous to the oral cavity (non-oral gnfr), as well as selected periodontal bacterial pathogens, were evaluated by culture in untreated and treated chronic periodontitis patients.201626835659
substrate distortion and the catalytic reaction mechanism of 5-carboxyvanillate decarboxylase.5-carboxyvanillate decarboxylase (ligw) catalyzes the conversion of 5-carboxyvanillate to vanillate in the biochemical pathway for the degradation of lignin. this enzyme was shown to require mn(2+) for catalytic activity and the kinetic constants for the decarboxylation of 5-carboxyvanillate by the enzymes from sphingomonas paucimobilis syk-6 (kcat = 2.2 s(-1) and kcat/km = 4.0 × 10(4) m(-1) s(-1)) and novosphingobium aromaticivorans (kcat = 27 s(-1) and kcat/km = 1.1 × 10(5) m(-1) s(-1)) were d ...201626714575
evaluation of the effectiveness of manual and automated dialyzers reprocessing after multiple reuses.a cross-sectional study was conducted to evaluate the effectiveness of manual and automated dialyzer reprocessing. dialyzers were filled with fluid thioglycollate medium from blood and dialysate chambers after being reprocessed and chemically sterilized with 0.2% peracetic acid. they were incubated for 14 days at 35°c ± 2°c, and microbiologic analysis was performed. microorganisms were identified in 3 of the 11 samples (27.3%) from the blood chambers: sphingomonas paucimobilis (2/3) and penicill ...201626897699
strategies for decreasing contamination of homemade nasal saline irrigation solutions.saline nasal irrigations (sni) are an important adjunct in the treatment of rhinosinusitis, and many patients prepare and store these solutions in their homes without an awareness of the potential for contamination. the objectives of this study were to determine if such contamination occurs and the effect of preparation methods on contamination.201626290494
bacterial and fungal contamination risks in human oocyte and embryo cryopreservation: open versus closed vitrification systems.to study the contamination risk in open and closed vitrification devices for oocyte/embryo cryopreservation by evaluating the contaminants present (bacteria and fungi) in the thaw medium and in liquid nitrogen (ln) storage containers.201627025548
draft genome sequence of sphingomonas paucimobilis strain lct-sp1 isolated from the shenzhou x spacecraft of china.sphingomonas paucimobilis strain lct-sp1 is a glucose-nonfermenting gram-negative, chemoheterotrophic, strictly aerobic bacterium. the major feature of strain lct-sp1, isolated from the chinese spacecraft shenzhou x, together with the genome draft and annotation are described in this paper. the total size of strain lct-sp1 is 4,302,226 bp with 3,864 protein-coding and 50 rna genes. the information gained from its sequence is potentially relevant to the elucidation of microbially mediated corrosi ...201626918090
purification and characterization of a fucoidanase (fnase s) from a marine bacterium sphingomonas paucimobilis pf-1.the search for enzyme activities that efficiently degrade marine polysaccharides is becoming an increasingly important area for both structural analysis and production of lower-molecular weight oligosaccharides. in this study, an endo-acting fucoidanase that degrades miyeokgui fucoidan (mf), a sulfated galactofucan isolated from the sporophyll (called miyeokgui in korean) of undaria pinnatifida, into smaller-sized galactofuco-oligosaccharides (1000-4000 da) was purified from a marine bacterium, ...201526193285
conjunctival flora of clinically normal and diseased turtles and tortoises.in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises.201525889261
[clinical observations of acute-onset endophthalmitis after clear corneal phacoemulsification].to evaluate the incidence of acute-onset endophthalmitis after clear corneal phacoemulsification and to report its clinical features, microbiology, and therapeutic efficacy.201526888274
[preliminary analyses on bacterial diversity and resistance in infection-related skin disorders].to explore the bacterial diversity and resistance in infection-related skin disorders.201526704163
microbiological stability of canned tuna produced in italy and in non-european countries.the results of a study on the microbiological stability of canned tuna produced by italian companies and similar canned products manufactured in countries outside europe are reported herein. the study involved 38 samples of canned tuna of various brands, of which 14 were produced by companies outside europe and 24 by italian companies. qualitative and quantitative microbiological tests were conducted for the following parameters: bacterial colony counts at 30°c, total coliforms, total enterobact ...201527800383
[frequency of colonization and isolated bacteria from the tip of the epidural catheter implanted for postoperative analgesia].the increased use of epidural analgesia with catheter leads to the need to demonstrate the safety of this method and know the incidence of catheter colonization, inserted postoperatively for epidural analgesia, and the bacteria responsible for this colonization.201525435414
posttraumatic sphingomonas paucimobilis endophthalmitis.a rare case of sphingomonas paucimobilis endophthalmitis secondary to a penetrating globe injury with a retained intraocular foreign body is presented. a 30-year-old man presented with severe pain following a penetrating left eye injury. visual acuity (va) was 6/120. slit-lamp examination revealed perforation of the temporal cornea and iris, hypopyon, and a fibrinous membrane covering the pupil. ultrasonography showed dense vitreous infiltration and an orbital ct-scan confirmed the presence of a ...201526839724
sphingomonas paucimobilis meningitis and ventriculitis in an immunocompromised host. 201526671080
combination of glycosphingosomes and liposomal doxorubicin shows increased activity against dimethyl-α-benzanthracene-induced fibrosarcoma in mice.the present study aimed to assess the antitumor effect of glycosphingolipid-incorporated liposomes (glycosphingosomes) in combination with liposomal doxorubicin (lip-dox) in a mouse model of fibrosarcoma. glycosphingosomes were prepared by incorporating glycosphingolipids isolated from sphingomonas paucimobilis into the liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, cholesterol, and cardiolipin. tumors were induced by administering dimethyl-α-benzanthracene, and tumor-bearing mice wer ...201526504383
theoretical study of the hydrolysis mechanism of 2-pyrone-4,6-dicarboxylate (pdc) catalyzed by ligi.2-pyrone-4,6-dicarboxylate lactonase (ligi) is the first identified enzyme from amidohydrolase superfamily that does not require a divalent metal ion for catalytic activity. it catalyzes the reversible hydrolysis of 2-pyrone-4,6-dicarboxylate (pdc) to 4-oxalomesaconate (oma) and 4-carboxy-2-hydroxymuconate (chm) in the degradation of lignin. in this paper, a combined quantum mechanics and molecule mechanics (qm/mm) approach was employed to study the reaction mechanism of ligi from sphingomonas p ...201526188792
sphingomonas zeae sp. nov., isolated from the stem of zea mays.a yellow-pigmented bacterial isolate (strain jm-791t) obtained from the healthy internal stem tissue of 1-month-old corn (zea mays, cultivar 'sweet belle') grown at the plant breeding unit of the e.v. smith research center in tallassee (elmore county), alabama, usa, was taxonomically characterized. the study employing a polyphasic approach, including 16s rna gene sequence analysis, physiological characterization, estimation of the ubiquinone and polar lipid patterns, and fatty acid composition, ...201525944808
biosynthesis and characterisation of silver nanoparticles using sphingomonas paucimobilis sp. bds1.sphingomonas is a novel and abundant microbial resource for biodegradation of aromatic compounds. it has great potential in environment protection and industrial production. the use of microorganisms for the synthesis of nanoparticles is in the limelight of modern nanotechnology, since it is cost effective, non-toxic and friendly to the ever-overwhelmed environment. in this paper, the biosynthesis of silver nanoparticles (agnps) using sphingomonas paucimobilis sp. bds1 under ambient conditions w ...201525829169
optimization of culture medium compositions for gellan gum production by a halobacterium sphingomonas paucimobilis.the effect of culture medium compositions on gellan gum production produced by fermentation with a halobacterium sphingomonas paucimobilis qhzjujw cgmcc2428 was studied. in this work, a fractional factorial design was applied to investigate the main factors that affected gellan gum production by s. paucimobilis qhzjujw cgmcc2428. sucrose was the best carbon source for gellan gum and peptone displayed better inducing effect. central composite design and response surface methodology were adopted t ...201525439950
sphingomonas aeria sp. nov. from indoor air of a pharmaceutical environment.a proteobacterial strain designated r1-3(t) was isolated from indoor air of a pharmaceutical environment. cells were gram-stain-negative, oxidase- and catalase-positive, aerobic, motile and rod-shaped. strain r1-3(t) grew optimally at ph 7, 30 °c and in 0-2 % nacl on r2a agar. the 16s rrna gene sequence analysis indicated that strain r1-3(t) belongs to the genus sphingomonas, and is closely related to sphingomonas paucimobilis atcc 29837(t) (98.4 % sequence similarity). however, the dna-dna rela ...201525318449
sphingomonas paucimobilis bacteraemia and shock in a patient with rheumatic carditis.acute rheumatic fever (arf) carditis is treated with steroids, which can cause changes in the cellular immune response, especially decreased cd3 (+) t cells. nosocomial infections due to steroid use for treatment of arf carditis or secondary to the changes in the cellular immune response have not been reported in the literature. sphingomonas paucimobilis is a gram-negative bacillus causing community- and hospital-acquired infections. it has been reported as causing bacteraemia/sepsis, pneumonia ...201425297038
growth-promoting sphingomonas paucimobilis zjsh1 associated with dendrobium officinale through phytohormone production and nitrogen fixation.growth-promoting sphingomonas paucimobilis zjsh1, associated with dendrobium officinale, a traditional chinese medicinal plant, was characterized. at 90 days post-inoculation, strain zjsh1 significantly promoted the growth of d. officinale seedlings, with increases of stems by 8.6% and fresh weight by 7.5%. interestingly, the polysaccharide content extracted from the inoculated seedlings was 0.6% higher than that of the control. similar growth promotion was observed with the transplants inoculat ...201425142808
a computational study of the dechlorination of β-hexachlorocyclohexane (β-hch) catalyzed by the haloalkane dehalogenase linb.linb, a haloalkane dehalogenase from sphingomonas paucimobilis ut26, is known to metabolize halohydrocarbons to halide ions and the respective alcohols. its broad substrate specificity allowed its consideration for bioremediation. herein, we have shown its catalytic action toward β-hexachlorocyclohexane (β-hch) - an example of large-size substrates that can be accommodated in its active site. we have analyzed the capability of combined qm/mm schemes to describe in detail the sn2 dechlorination r ...201425102308
fever of unknown origin in a young man.sphingomonas paucimobilis bacteraemia is a rare infection typically related to nosocomial outbreaks.201424852662
sphingomonas paucimobilis: an unusual cause of meningitis-case report.sphingomonas paucimobilis is an aerobic gram-negative bacillus. the bacteria can cause infections, which can be devastating and, therefore, the patients need adequate and early antibiotic cover. we are presenting an interesting case of meningitis secondary to an unusual s. paucimobilis infection. this is the second case to our knowledge in the literature on meningitis due to s. paucimobilis. the 31-year-old previously healthy man presented with 2 months' history of weight loss and loss of appeti ...201424201101
enhanced gellan gum production by hydrogen peroxide (h2o 2) induced oxidative stresses in sphingomonas paucimobilis.in this study, the effect of h2o2-induced oxidative stress on gellan gum production and cell growth were investigated. gellan gum production was improved and cell growth was inhibited by h2o2. a multiple h2o2 stresses with different concentrations were developed to optimize gellan gum production. a maximal gellan gum yield (22.52 g/l), which was 35.58 % higher than the control, was observed with 2, 2, 3, 4 mmol/l h2o2 added at 6, 12, 18, 24 h, respectively. moreover, udp-glucose pyrophosphorylas ...201423925529
prevalence of antibacterial resistant bacterial contaminants from mobile phones of hospital inpatients.mobile phones contaminated with bacteria may act as fomites. antibiotic resistant bacterial contamination of mobile phones of inpatients was studied. one hundred and six samples were collected from mobile phones of patients admitted in various hospitals in jazan province of saudi arabia. eighty-nine (83.9%) out of 106 mobile phones were found to be contaminated with bacteria. fifty-two (49.0%) coagulase-negative staphylococcus, 12 (11.3%) staphylococcus aureus, 7 (6.6%) enterobacter cloacae, 3 ( ...201428156258
prevalence of antibacterial resistant bacterial contaminants from mobile phones of hospital inpatients.mobile phones contaminated with bacteria may act as fomites. antibiotic resistant bacterial contamination of mobile phones of inpatients was studied. one hundred and six samples were collected from mobile phones of patients admitted in various hospitals in jazan province of saudi arabia. eighty-nine (83.9%) out of 106 mobile phones were found to be contaminated with bacteria. fifty-two (49.0%) coagulase-negative staphylococcus, 12 (11.3%) staphylococcus aureus, 7 (6.6%) enterobacter cloacae, 3 ( ...201425292217
in silico detection of virulence gene homologues in the human pathogen sphingomonas spp.there is an ongoing debate about the clinical significance of sphingomonas paucimobilis as a virulent bacterial pathogen. in the present study, we investigated the presence of different virulence factors and genes in sphingomonas bacteria. we utilized phylogenetic, comparative genomics and bioinformatics analysis to investigate the potentiality of sphingomonas bacteria as virulent pathogenic bacteria. the 16s ribosomal rna gene (16s rdna) phylogenetic tree showed that the closest bacterial taxon ...201425574122
nonfermenting gram-negative bacilli other than pseudomonas aeruginosa and acinetobacter spp. causing respiratory tract infections in a tertiary care center.nonfermenting gram-negative bacilli have emerged as important healthcare-associated pathogens. it is important to correctly identify all clinically significant nonfermenting gram-negative bacilli considering the intrinsic multidrug resistance exhibited by these bacteria.201324672175
sanitization of an automatic reverse-osmosis watering system: removal of a clinically significant biofilm.during environmental monitoring of our institution's rodent watering systems, one vivarium was found to have high bacterial loads in the reverse-osmosis (ro) automatic water system. these findings prompted evaluation of the entire ro water production and distribution system. investigation revealed insufficient rack and ro system sanitization, leading to heavy biofilm accumulation within the system. approximately 2 wk after discovery in the water system, one of the bacterial organisms isolated in ...201323562105
community acquired bacteremia by sphingomonas paucimobilis: two rare case reports.s.paucimobilis has a diverse nutritional substrate spectrum and found in both environmental and hospital settings. sphingomonas paucimobilis is rarely isolated from clinical specimen. this low virulence organism since has been reported to cause a variety of diseases since 1979. it has been reported to be associated with both community acquired and nosocomial diseases including bacteremia, catheter related sepsis, diarrhoeal diseases, peritonitis, meningitis, cutaneous infections, endopthalmitis, ...201324551684
characterizing the promiscuity of ligab, a lignin catabolite degrading extradiol dioxygenase from sphingomonas paucimobilis syk-6.ligab from sphingomonas paucimobilis syk-6 is the only structurally characterized dioxygenase of the largely uncharacterized superfamily of type ii extradiol dioxygenases (edo). this enzyme catalyzes the oxidative ring-opening of protocatechuate (3,4-dihydroxybenzoic acid or pca) in a pathway allowing the degradation of lignin derived aromatic compounds (ldacs). ligab has also been shown to utilize two other ldacs from the same metabolic pathway as substrates, gallate, and 3-o-methyl gallate; ho ...201323977959
engineering bacteria for bioremediation of persistent organochlorine pesticide lindane (γ-hexachlorocyclohexane).strategies were designed for bioremediation of the highly persistent toxic pesticide γ-hexachlorocyclohexane (γ-hch) or lindane from the environment. lindane caused the loss of stress-protective chaperone groel, and inhibited photosynthesis, respiration and nitrogen-fixation in anabaena, resulting in growth arrest. to alleviate lindane toxicity, the lina2 gene, encoding hch dehydrochlorinase from sphingomonas paucimobilis b90, was knocked-in at an innocuous locus in anabaena genome and over-expr ...201324135568
an evaluation of microbial and chemical contamination sources related to the deterioration of tap water quality in the household water supply system.the predominant microorganisms in samples taken from shower heads in residences in the korean city "n" were stenotrophomonas maltophilia, sphingomonas paucimobilis, acidovorax temperans, and microbacterium lacticum. legionella was not detected in this case. the volatile organic compounds (vocs) vinylacetate, nn-dma, cis-1,2-dichloroethylene, epichlorohydrin, and styrene were measured in five types of plastic pipes: pvc, pb, pp, pe, and cpvc. the rate of multiplication of the heterotrophic plate ...201324018837
[septic monarthritis caused by sphingomonas paucimobilis].sphingomonas paucimobilis, formerly known as pseudomonas paucimobilis, is a rare cause of septic arthritis and is therefore regarded as being of minor clinical interest in rheumatological diagnostics. in this connection the yellow pigmented, aerobic, glucose non-fermenting, gram negative bacillus is usually associated with immunocompromised patients. a case of septic arthritis in a 70-year-old man with chronic obstructive pulmonary disease (copd) initially presenting with right knee pain, swelli ...201323921948
sphingomonas paucimobilis infections in children: 24 case reports.sphingomonas paucimobilis is a causative agent of infection in immunocompromised patients, and healthcare-associated infection. although the infections associated with s.paucimobilis occurs rarely, it has been encountered with increasing frequency in clinical settings. in the current study we reported clinical features of the children with s.paucimobilis infection, and the antimicrobial susceptibilities of the isolated strains among the patients. this study was conducted in dr. behçet uz childre ...201323795278
an insight into the emerging exopolysaccharide gellan gum as a novel polymer.the microbial exopolysaccharides are water-soluble polymers secreted by microorganisms during fermentation. the biopolymer gellan gum is a relatively recent addition to the family of microbial polysaccharides that is gaining much importance in food, pharmaceutical and chemical industries due to its novel properties. it is commercially produced by c.p. kelco in japan and the usa. this article presents a critical review of the available information on the gum synthesized by sphingomonas paucimobil ...201323499110
[sphingomonas paucimobilis]. 201323450409
sphingomonas paucimobilis osteomyelitis in an immunocompetent patient. a rare case report and literature review.sphingomonas paucimobilis occurs widely both in natural and nosocomial environments, including hospital water systems, respiratory therapy equipment, and laboratory instruments. it is an opportunistic pathogen that rarely causes infections in humans. among s. paucimobilis nosocomial infections, osteomyelitis is particularly rare. almost all infections occur in patients with comorbidities such as malignancy, immunosuppressant therapy, diabetes mellitus and acquired immunodeficiency syndrome. we p ...201324177306
the pyridoxal 5'-phosphate (plp)-dependent enzyme serine palmitoyltransferase (spt): effects of the small subunits and insights from bacterial mimics of human hlcb2a hsan1 mutations.the pyridoxal 5'-phosphate (plp)-dependent enzyme serine palmitoyltransferase (spt) catalyses the first step of de novo sphingolipid biosynthesis. the core human enzyme is a membrane-bound heterodimer composed of two subunits (hlcb1 and hlcb2a/b), and mutations in both hlcb1 (e.g., c133w and c133y) and hlcb2a (e.g., v359m, g382v, and i504f) have been identified in patients with hereditary sensory and autonomic neuropathy type i (hsan1), an inherited disorder that affects sensory and autonomic ne ...201324175284
a new strategy to enhance gellan production by two-stage culture in sphingomonas paucimobilis.the effects of different initial sucrose concentrations and temperatures on gellan biosynthesis by sphingomonas paucimobilis atcc 31461 were investigated. lower sucrose concentrations and higher temperatures were favorable for cell growth. higher sucrose concentrations and lower temperatures promoted gellan production but retarded cell growth. based on these results, a two-stage culture strategy was developed to improve gellan production. during the first 24 h, s. paucimobilis was cultured in a ...201323987418
the draft genome sequence of sphingomonas paucimobilis strain her1398 (proteobacteria), host to the giant pau phage, indicates that it is a member of the genus sphingobacterium (bacteroidetes).the draft genome sequence of sphingomonas paucimobilis host index number (her) 1398, host of the giant pau phage isolated from silk moths (bombyx mori), indicates that this isolate belongs within the genus sphingobacterium. we suggest that sphingomonas paucimobilis strain her1398 be reclassified as sphingobacterium paucimobilis strain her1398.201323929486
postpartum fever in the presence of a fibroid: sphingomonas paucimobilis sepsis associated with pyomyoma.pyomyoma is a life-threatening complication of uterine leiomyoma. it may occur in post- menopausal women, during pregnancy and in the postpartum period. fever may be the only manifestation during the early stages of the disease. we detail the first reported case of postpartum pyomyoma-related sepsis due to sphingomonas paucimobilis, a gram-negative bacillus that is gaining recognition as an important human pathogen.201324308831
sphingomonas starnbergensis sp. nov., isolated from a prealpine freshwater lake.a novel type of freshwater bacterium was isolated from the prealpine mesotrophic starnberger see (bavaria, southern germany). cells of strain 382(t) were gram-negative and rod-shaped, motile and creamy-white. the isolate was strictly aerobic, catalase- and oxidase-positive, and grew at ph values of 6-9 (optimum, ph 7) and temperatures of 10-37 °c (optimum, 28 °c). the genomic g+c content of strain 382(t) was 64.1 mol%. based on 16s rrna gene sequence analyses, strain 382(t) belongs to the family ...201322685105
dynamic passive dosing for studying the biotransformation of hydrophobic organic chemicals: microbial degradation as an example.biotransformation plays a key role in hydrophobic organic compound (hoc) fate, and understanding kinetics as a function of (bio)availability is critical for elucidating persistence, accumulation, and toxicity. biotransformation mainly occurs in an aqueous environment, posing technical challenges for producing kinetic data because of low hoc solubilities and sorptive losses. to overcome these, a new experimental approach based on passive dosing is presented. this avoids using cosolvent for introd ...201222458885
septic arthritis caused by sphingomonas paucimobilis in an immunocompetent patient. 201222902983
molecular and microscopic analysis of bacteria and viruses in exhaled breath collected using a simple impaction and condensing method.exhaled breath condensate (ebc) is increasingly being used as a non-invasive method for disease diagnosis and environmental exposure assessment. by using hydrophobic surface, ice, and droplet scavenging, a simple impaction and condensing based collection method is reported here. human subjects were recruited to exhale toward the device for 1, 2, 3, and 4 min. the exhaled breath quickly formed into tiny droplets on the hydrophobic surface, which were subsequently scavenged into a 10 µl rolling de ...201222848436
a biofilm model developed to investigate survival and disinfection of mycobacterium mucogenicum in potable water.water in healthcare environments can be a source for healthcare-associated infections (hai). however, information on the exposure risk to opportunistic pathogens in potable water distribution systems (pwds) is lacking. laboratory studies characterizing the interaction of opportunistic pathogens with biofilms are needed to understand their role in water systems within healthcare facilities. a stable, repeatable, pwds multi-species biofilm model comprising sphingomonas paucimobilis, methylobacteri ...201223082863
liver abscess caused by brevundimonas vesicularis in an immunocompetent patient.invasive infections caused by brevundimonas vesicularis are very rare in humans. we experienced an unusual case of liver abscess due to b. vesicularis in an immunocompetent young male. the patient was successfully treated by liver abscess drainage and with antimicrobial therapy of ceftriaxone followed by ampicillin/sulbactam. the organism found in the aspiration culture of the abscess material was initially reported, by using a vitek 2 system, as sphingomonas paucimobilis. however, later, b. ves ...201222767540
analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary pcr-restriction fragment length polymorphism fingerprint database for identifying isolates.twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. the bacteria identified were brevibacillus laterosporus, leclercia adecarboxylata, peptococcus niger, bacillus circulans, raoultella ornithinolytica, bacillus subtilis, bacillus cereus, bacillus thermobacillus, bacillus choshinensis, bacillus sphaericus, acinetobacter haemolyticus, sphingomonas paucimobilis, bacillus thuringiensis, staphylo ...201223451394
structure and catalytic mechanism of ligi: insight into the amidohydrolase enzymes of cog3618 and lignin degradation.ligi from sphingomonas paucimobilis catalyzes the reversible hydrolysis of 2-pyrone-4,6-dicarboxylate (pdc) to 4-oxalomesaconate and 4-carboxy-2-hydroxymuconate in the degradation of lignin. this protein is a member of the amidohydrolase superfamily of enzymes. the protein was expressed in escherichia coli and then purified to homogeneity. the purified recombinant enzyme does not contain bound metal ions, and the addition of metal chelators or divalent metal ions to the assay mixtures does not a ...201222475079
diversity of bacteria nesting the plant cover of north sinai deserts, egypt.north sinai deserts were surveyed for the predominant plant cover and for the culturable bacteria nesting their roots and shoots. among 43 plant species reported, 13 are perennial (e.g. fagonia spp., pancratium spp.) and 30 annuals (e.g. bromus spp., erodium spp.). eleven species possessed rhizo-sheath, e.g. cyperus capitatus, panicum turgidum and trisetaria koelerioides. microbiological analyses demonstrated: the great diversity and richness of associated culturable bacteria, in particular nitr ...201225685397
monitoring of antibiotic resistance in bacteria isolated from laboratory animals.the drug resistance of microorganisms isolated from laboratory animals never treated with antibiotics is being reported consistently, while the number of laboratory animals used in medicine, pharmacy, veterinary medicine, agriculture, nutrition, and environmental and health science has increased rapidly in korea. therefore, this study examined the development of antimicrobial resistance in bacteria isolated from laboratory animals bred in korea. a total of 443 isolates (7 species) containing 5 s ...201222787489
comparative study of bacterial status from conjunctival sac of the elder qiang minority and han people with dry eye in sichuan, china.to compare the status of bacteria in the conjunctival sac from the elder qiang minority and han people with dry eyes in sichuan, china.201222773985
removal of triphenylmethane dyes by bacterial consortium.a new consortium of four bacterial isolates (agrobacterium radiobacter; bacillus spp.; sphingomonas paucimobilis, and aeromonas hydrophila)-(cm-4) was used to degrade and to decolorize triphenylmethane dyes. all bacteria were isolated from activated sludge extracted from a wastewater treatment station of a dyeing industry plant. individual bacterial isolates exhibited a remarkable color-removal capability against crystal violet (50 mg/l) and malachite green (50 mg/l) dyes within 24 h. interestin ...201222623907
a case of peritoneal dialysis-associated peritonitis caused by sphingomonas paucimobilis.sphingomonas paucimobilis is an aerobic gram-negative bacillus found in soil and water. knowledge regarding the role of this infectious agent is limited because it is rarely isolated from human material. furthermore, it is an unusual pathogen in cases of peritoneal dialysis (pd)-associated peritonitis. the clinical courses and outcomes of peritonitis caused by s. paucimobilis are variable. whereas some patients were cured with appropriate antibiotic therapy, others required catheter removal. cas ...201226877918
[sphingomonas paucimobilis infection with underlying submandibular sialolithiasis].sphingomonas paucimobilis (s. paucimobilis), is a gram-negative, aerobic, non-fermentative, oxidase (+) and catalase (+) bacterium. although s. paucimobilis is isolated very rarely, it can cause both nosocomial and community-acquired infections. a patient admitted to our clinic had a complaint of swelling in the right mandibular region and pain increasing while eating for the previous week. bimanual palpation revealed a painful swelling of 1x1x1 cm in size inside the right wharton's duct. via ma ...201121303318
a rare case of peripartum endogenous bacterial endophthalmitis.we describe the case of a healthy, pregnant female who developed endogenous endophthalmitis at the time of delivery, and discuss the possible mechanism of infection and the management of this case. a 26-year-old asian woman presented with a 3-week history of visual deterioration and pain in the right eye. there was no history of ocular trauma or surgery. the ocular symptoms developed one day after vaginal delivery of a healthy baby. the pregnancy had been uncomplicated until premature rupture of ...201121327678
application of the knowledge-based approach to strain selection for a bioaugmentation process of phenanthrene- and cr(vi)-contaminated soil.aims:  the objective of this study was to apply the knowledge-based approach to the selection of an inoculum to be used in bioaugmentation processes to facilitate phenanthrene degradation in phenanthrene- and cr(vi)-co-contaminated soils. methods and results:  the bacterial community composition of phenanthrene and phenanthrene- and cr(vi)-co-contaminated microcosms, determined by denaturing gradient gel electrophoresis analysis, showed that members of the sphingomonadaceae family were the predo ...201121518157
risk factors associated with sphingomonas paucimobilis infection.background: sphingomonas paucimobilis is rarely isolated from clinical specimens and it is associated with a great variety of infections. the aim of this study is to investigate the microbiological and clinical features of s paucimobilis infection in southern taiwan. methods: s paucimobilis isolates from the microbiology laboratory of chi-mei medical center and their relevant clinical data from october 2005 to october 2009 were retrospectively reviewed. results: a total of 55 patients with docum ...201121524965
synergistic effects and physiological responses of selected bacterial isolates from animal feed to four natural antimicrobials and two antibiotics.abstract in this study, 20 samples from three different sources of animal feed were investigated and six bacterial isolates were identified. the susceptibility of four natural antimicrobials, namely, eugenol, cinnamaldehyde, thymol, and carvacrol, against six of these isolates was determined. carvacrol and eugenol showed better inhibitory effects with larger zones of inhibition. the minimal inhibitory concentration for a range of antibiotics on the susceptibility of two isolates (namely, sphingo ...201121612425
crystal structure of h2o2-dependent cytochrome p450sp{alpha} with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the {alpha} position.cytochrome p450(sp+¦) (cyp152b1) isolated from sphingomonas paucimobilis is the first p450 to be classified as a h(2)o(2)-dependent p450. p450(sp+¦) hydroxylates fatty acids with high +¦-regioselectivity. herein we report the crystal structure of p450(sp+¦) with palmitic acid as a substrate at a resolution of 1.65 ôä½. the structure revealed that the c(+¦) of the bound palmitic acid in one of the alternative conformations is 4.5 ôä½ from the heme iron. this conformation explains the highly selec ...201121719702
outbreak of sphingomonas paucimobilis septicemia in a neonatal intensive care unit.we describe an outbreak of sphingomonas paucimobilis in 13 newborn infants with septicemia and septic shock. fifteen available isolates were obtained from patients and distilled water. pulsed-field gel electrophoresis results pointed out that there was a cross-transmission of s. paucimobilis in eleven patients but these types were not the same the isolate obtained from the distilled water. the outbreak was terminated by taking appropriate control measures for infection and change of source of di ...201121719938
fine tuning by human cd1e of lipid-specific immune responses.cd1e is a member of the cd1 family that participates in lipid antigen presentation without interacting with the t-cell receptor. it binds lipids in lysosomes and facilitates processing of complex glycolipids, thus promoting editing of lipid antigens. we find that cd1e may positively or negatively affect lipid presentation by cd1b, cd1c, and cd1d. this effect is caused by the capacity of cd1e to facilitate rapid formation of cd1-lipid complexes, as shown for cd1d, and also to accelerate their tur ...201121844346
sphingomonas paucimobilis bacteremia related to intravenous human immunoglobulin injections. 201122075255
Sphingomonas alpina sp. nov., a psychrophilic bacterium isolated from alpine soil.An aerobic, Gram-negative, motile, psychrophilic bacterium, designated strain S8-3T, was isolated from alpine soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S8-3T was related to the genus Sphingomonas and had highest 16S rRNA gene sequence similarity with Sphingomonas oligophenolica S213T (98.0 %). 16S RNA gene sequence similarity between strain S8-3T and Sphingomonas paucimobilis ATCC 29837 T (the type species of the genus Sphingomonas) was 93.0 %. Strain S8-3T ...201121856979
[Endogenous postpartum panophthalmitis induced by sphingomonas paucimobili].Endogenous panophthalmitis is a rare eye disease with purulent necrosis of all the ocular structures. It is a rare but serious condition that occurs when bacteria cross the blood-ocular barrier and multiply within the eye. Although rare, endogenous panophthamitis is a potentially devastating intraocular infection resulting in a poor visual and anatomic prognosis. We present a 39-year-old woman, without any previous history, who developed a endogenous panophthalmitis in the left eye secondary to ...201122003763
the effect of sterile versus non-sterile tourniquets on microbiological colonisation in lower limb surgery.surgical tourniquets are commonplace in lower limb surgery. several studies have shown that tourniquets can be a potential source of microbial contamination but have not compared the use of sterile versus non-sterile tourniquets in the same procedures.201122041233
a rare cause of bacteremia in a pediatric patient with down syndrome: sphingomonas paucimobilis.sphingomonas paucimobilis, is a yellow-pigmented, aerobic, non fermentative, gram negative motile bacillus. s. paucimobilis which is widely found in nature and hospital environments rarely cause serious or life threatening infections. in this report, a case of hospital acquired bloodstream infection due to s. paucimobilis in a patient with down syndrome who was on treatment for presumed pneumonia is presented. a one year-old child patient who was a known case of down syndrome and had previously ...201121960744
food-borne bacteremic illnesses in febrile neutropenic children.bacteremia following febrile neutropenia is a serious complication in children with malignancies. preventive measures are currently targeted at antimicrobial prophylaxis, amelioration of drug-induced neutropenia, and nosocomial spread of pathogens, with little attention to community-acquired infections. a retrospective study was conducted at a pediatric oncology center during a 3-year period to identify probable cases of food-borne infections with bacteremia. twenty-one bacteremic illnesses affe ...201122184532
Enzymes involved in the biodegradation of hexachlorocyclohexane: A mini review.The scope of this paper encompasses the following subjects: (i) aerobic and anaerobic degradation pathways of ?-hexachlorocyclohexane (HCH); (ii) important genes and enzymes involved in the metabolic pathways of ?-HCH degradation; (iii) the instrumental methods for identifying and quantifying intermediate metabolites, such as gas chromatography coupled to mass spectrometry (GC-MS) and other techniques. It can be concluded that typical anaerobic and aerobic pathways of ?-HCH are well known for a ...201121992990
[bacterial profiles in conjunctival sac of middle-aged and elderly qiang with dry eyes and normal eyes].to compare the bacteria profiles in conjunctival sac of qiang aged 40 years and over between those with dry eyes and those with normal eyes.201122332548
Refresh
firstprev
12345
nextlast
Displaying items 1 - 100 of 452