Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| rnai-mediated resistance to rice black-streaked dwarf virus in transgenic rice. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, causes significant economic losses in rice production in china and many other asian countries. development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to rbsdv have not yet been found. one of the most promising methods to confer resistance against rbsdv is the use of rna interference (rnai) technology. rbsdv non-structural pro ... | 2017 | 27900537 |
| characterization of small interfering rnas derived from rice black streaked dwarf virus in infected maize plants by deep sequencing. | rice black streaked dwarf virus (rbsdv) is the casual agent of maize rough dwarf disease, which frequently causes severe yield loss in china. however, the interaction between rbsdv and maize plants is largely unknown. rna silencing is a conserved mechanism against viruses in plants. to understand the antiviral rna interfering response in rbsdv-infected plants, the profile of virus-derived small interfering rnas (vsirnas) from rbsdv in infected maize plants was obtained by deep sequencing in this ... | 2017 | 27888127 |
| structure and components of the globular and filamentous viroplasms induced by rice black-streaked dwarf virus. | viroplasms of members of the family reoviridae are considered to be viral factories for genome replication and virion assembly. globular and filamentous phenotypes have different components and probably have different functions. we used transmission electron microscopy and electron tomography to examine the structure and components of the two viroplasm phenotypes induced by rice black-streaked dwarf virus (rbsdv). immuno-gold labeling was used to localize each of the 13 rbsdv encoded proteins as ... | 2017 | 28359957 |
| the influence of sogatella furcifera (hemiptera: delphacidae) migratory events on the southern rice black-streaked dwarf virus epidemics. | southern rice black-streaked dwarf virus (srbsdv) caused serious rice losses. after the first outbreak in 2009 in northern vietnam and southern china, the virus ravaged crops again on enormous scales in 2010, but infections have decreased sharply since 2011. we presumed that the sudden epidemics and fadeout of srbsdv would be closely related to the migratory events of the insect vector, sogatella furcifera. this study sought the source area of srbsdv using the trajectory analysis method, and rev ... | 2017 | 28334380 |
| evaluation of rice resistance to southern rice black-streaked dwarf virus and rice ragged stunt virus through combined field tests, quantitative real-time pcr, and proteome analysis. | diseases caused by southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) considerably decrease grain yield. therefore, determining rice cultivars with high resistance to srbsdv and rrsv is necessary. in this study, rice cultivars with high resistance to srbsdv and rrsv were evaluated through field trials in shidian and mangshi county, yunnan province, china. sybr green i-based quantitative real-time polymerase chain reaction (qrt-pcr) analysis was used to quantitat ... | 2017 | 28241456 |
| characteristics of sirnas derived from southern rice black-streaked dwarf virus in infected rice and their potential role in host gene regulation. | virus-derived sirnas (vsirnas)-mediated rna silencing plays important roles in interaction between plant viruses and their hosts. southern rice black-streaked dwarf virus (srbsdv) is a newly emerged devastating rice reovirus with ten dsrna genomic segments. the characteristics of srbsdv-derived sirnas and their biological implications in srbsdv-rice interaction remain unexplored. | 2017 | 28183327 |
| interaction between southern rice black-streaked dwarf virus minor core protein p8 and a rice zinc finger transcription factor. | the fijivirus southern rice black-streaked dwarf virus (srbsdv) causes one of the most serious viral diseases of rice in china and vietnam. to better understand the molecular basis of srbsdv infection, a yeast two-hybrid screen of a rice cdna library was carried out using p8, a minor core protein of srbsdv, as the bait. a rice cys2his2-type zinc finger protein (oszfp) was found to interact with srbsdv p8. a strong interaction between srbsdv p8 and oszfp was then confirmed by pull-down assays, an ... | 2017 | 28124144 |
| jasmonic acid-mediated defense suppresses brassinosteroid-mediated susceptibility to rice black streaked dwarf virus infection in rice. | plant hormones play a vital role in plant immune responses. however, in contrast to the relative wealth of information on hormone-mediated immunity in dicot plants, little information is available on monocot-virus defense systems. we used a high-throughput-sequencing approach to compare the global gene expression of rice black-streaked dwarf virus (rbsdv)-infected rice plants with that of healthy plants. exogenous hormone applications and transgenic rice were used to test rbsdv infectivity and p ... | 2017 | 27976810 |
| interaction research on an antiviral molecule that targets the coat protein of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) coat protein (p10) is the key protein required for viral transmission and host plant infection and is thus a promising target for anti-srbsdv agent screening. in this study, p10 was obtained from escherichia coli through cloning, expression, and purification. the antiviral agent ningnanmycin was selected as control, and a series of antiviral compounds based on the structural scaffold of ferulic acid were analyzed. size-exclusion chromatography an ... | 2017 | 28522394 |
| development of a simplified rt-pcr without rna isolation for rapid detection of rna viruses in a single small brown planthopper (laodelphax striatellus fallén). | the small brown planthopper (sbph) is an important pest of cereal crops and acts as a transmission vector for multiple rna viruses. rapid diagnosis of virus in the vector is crucial for efficient forecast and control of viral disease. reverse transcription polymerase chain reaction (rt-pcr) is a rapid, sensitive and reliable method for virus detection. the traditional rt-pcr contains a rna isolation step and is widely used for virus detection in insect. however, using the traditional rt-pcr for ... | 2017 | 28468626 |
| co-infection of two reoviruses increases both viruses accumulation in rice by up-regulating of viroplasm components and movement proteins bilaterally and rna silencing suppressor unilaterally. | synergism between southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) not only aggravates disease symptoms but also enhances their vector acquisition efficiencies by increasing both viruses' titers in co-infected rice plants, which may exacerbate the epidemic of both viruses and cause significant damage to rice production. the molecular mechanism of viral synergism of these two viruses remains unexplored. | 2017 | 28789694 |
| a novel method for transmitting southern rice black-streaked dwarf virus to rice without insect vector. | southern rice black-streaked dwarf virus (srbsdv) has spread from the south of china to the north of vietnam in the past few years, and has severely influenced rice production. however, previous study of traditional srbsdv transmission method by the natural virus vector, the white-backed planthopper (wbph, sogatella furcifera), in the laboratory, researchers are frequently confronted with lack of enough viral samples due to the limited life span of infected vectors and rice plants and low virus ... | 2017 | 28810884 |
| transcriptional changes of rice in response to rice black-streaked dwarf virus. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, causes significant economic losses in rice production in china and many other asian countries. although a great deal of effort has been made to elucidate the interactions among the virus, insect vectors, host and environmental conditions, few rbsdv proteins involved in pathogenesis have been identified, and the biological basis of disease development in rice remains largely unknown. transcriptomic ... | 2017 | 28700950 |
| label-free quantitative proteomic analysis of chitosan oligosaccharide-treated rice infected with southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) has spread from thesouth of china to the north of vietnam in the past few years and severelyinfluenced rice production. its long incubation period and early symptoms are not evident; thus, controlling it is difficult. chitosan oligosaccharide (cos) is a green plant immunomodulator. early studies showed that preventing and controlling srbsdv have a certain effect and reduce disease infection rate, but its underlying controlling and preventing mech ... | 2017 | 28524115 |
| rice black streaked dwarf virus p7-2 forms a scf complex through binding to oryza sativa skp1-like proteins, and interacts with gid2 involved in the gibberellin pathway. | as a core subunit of the scf complex that promotes protein degradation through the 26s proteasome, s-phase kinase-associated protein 1 (skp1) plays important roles in multiple cellular processes in eukaryotes, including gibberellin (ga), jasmonate, ethylene, auxin and light responses. p7-2 encoded by rice black streaked dwarf virus (rbsdv), a devastating viral pathogen that causes severe symptoms in infected plants, interacts with skp1 from different plants. however, whether rbsdv p7-2 forms a s ... | 2017 | 28494021 |
| bacterial microbiota in small brown planthopper populations with different rice viruses. | the small brown planthopper (sbph) is an important virus vector, transmitting rice stripe virus (rsv), and rice black-streaked dwarf virus (rbsdv). insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by sbph. here, we investigated endosymbiont communities in non-viruliferous, rsv-infected, and rbsdv-infected sbph populations using illumina 16s rrna gene miseq sequencing. in total, 281,803 effective sequences ... | 2017 | 28471518 |
| argonaute subfamily genes in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | argonaute (ago) proteins are essential catalytic components of the rna-induced silencing complex and play central roles in rna interference. using a combination of bioinformatics and rapid amplification of cdna ends (race) methods, putative ago subfamily members, ls-ago1 and ls-ago2, were cloned and characterized from the small brown planthopper, laodelphax striatellus. the open reading frame (orf) of ls-ago1 is 2,820 bp long, encoding a putative protein of 939 amino acid residues, and ls-ago2 c ... | 2016 | 26446351 |
| low temperature storage of southern rice black-streaked dwarf virus-infected rice plants cannot sustain virus transmission by the vector. | southern rice black-streaked dwarf virus (srbsdv) is a novel virus transmitted by white-backed planthopper sogatella furcifera (hováth) (hemiptera: delphacidae). due to low virus transmission efficiency by the planthopper, researchers are frequently confronted with shortage of viruliferous vectors or infected rice plants, especially in winter and the following spring. to find new ways to maintain virus-infected materials, viral rice plants were stored at -80°c for 45 or 140 d and evaluated as vi ... | 2016 | 26405060 |
| rice responses and resistance to planthopper-borne viruses at transcriptomic and proteomic levels. | rice (oryza sativa) is one of the most important cereal crops in the world, especially in asian areas. rice virus diseases are considered as the most serious threat to rice yields. most rice viruses are transmitted by hemipteran insects such as planthoppers and leafhoppers. in asia five rice viruses are transmitted mainly by three planthopper species in a persistent manner: rice stripe virus, rice black-streaked dwarf virus, rice ragged stunt virus, rice grassy stunt virus, and southern rice bla ... | 2016 | 26363817 |
| virus altered rice attractiveness to planthoppers is mediated by volatiles and related to virus titre and expression of defence and volatile-biosynthesis genes. | viruses may induce changes in plant hosts and vectors to enhance their transmission. the white-backed planthopper (wbph) and brown planthopper (bph) are vectors of southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv), respectively, which cause serious rice diseases. we herein describe the effects of srbsdv and rrsv infections on host-selection behaviour of vector and non-vector planthoppers at different disease stages. the y-tube olfactometer choice and free-choic ... | 2016 | 27924841 |
| complete genomic sequence of maize rough dwarf virus, a fijivirus transmitted by the small brown planthopper. | the nucleotide sequences of the 10 genomic segments of an italian isolate of maize rough dwarf virus (mrdv) were determined. this first complete genomic sequence of mrdv will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize. | 2016 | 26847903 |
| reference gene selection and evaluation for gene expression studies using qrt-pcr in the white-backed planthopper, sogatella furcifera (hemiptera: delphacidae). | the white-backed planthopper, sogatella furcifera (hemiptera, delphacidae), is one of the most devastating rice pests. for a better control strategy, various genetic studies have been conducted using reverse-transcription quantitative real-time polymerase chain reaction (qrt-pcr). the appropriate application of qrt-pcr requires reliable endogenous controls; however, studies on this aspect of the white-backed planthopper are lacking. in the present study, nine commonly used reference genes, elong ... | 2016 | 26612891 |
| the speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors. | rice black-streaked dwarf virus (rbsdv) and southern rice black-streaked dwarf virus (srbsdv) are two closely related fijiviruses transmitted by the small brown planthopper (sbph) and white-backed planthopper (wbph), respectively. srbsdv has a latent period 4 days shorter than that of rbsdv, implying a more efficient spread in insect vector. currently, the mechanisms underlying this higher efficiency are poorly understood. however, our recent studies have implicated a role of virus induced tubul ... | 2016 | 27760544 |
| asymmetric spread of srbsdv between rice and corn plants by the vector sogatella furcifera (hemiptera: delphacidae). | plant viruses are mostly transmitted by sucking insects via their piercing behaviors, which may differ due to host plant species and their developmental stages. we characterized the transmission of a fijivirus, southern rice black-streaked dwarf virus (srbsdv), by the planthopper vector sogatella furcifera horváth (hemiptera: delphacidae), between rice and corn plants of varying developmental stages. srbsdv was transmitted from infected rice to uninfected corn plants as efficiently as its transm ... | 2016 | 27760223 |
| molecular variation and expansion of a rice black-streaked dwarf virus population based on analysis of segment 1 in jining, china. | to analyze the variation in rice black-streaked dwarf virus (rbsdv) in an area with high incidence of maize rough dwarf disease (mrdd), the rbsdv s1 segment in a collection of 100 maize isolates (sample population a100) from jining, shandong province, was sequenced. an additional 21 maize and rice isolates (subpopulation b21) that were sampled from nine other geographic locations in china in 2012 and 2013 were used as a control. a total of 914 nucleotide mutations, including 239 singleton variab ... | 2016 | 27638775 |
| tolerance and responsive gene expression of sogatella furcifera under extreme temperature stresses are altered by its vectored plant virus. | southern rice black-streaked dwarf virus (srbsdv), a newly emerged fijivirus causing great loss to rice production in eastern and southeastern asian countries in recent years, is efficiently transmitted by a rice pest, white-backed planthopper (wbph, sogatella furcifera) in a persistent, circulative propagative manner and can be considered as an insect virus. in this study, srbsdv infection in wbph was found to increase the vector's death rate under extreme cold stress but improve its survival r ... | 2016 | 27531640 |
| dual transcriptome analysis reveals insights into the response to rice black-streaked dwarf virus in maize. | maize rough dwarf disease (mrdd) is a viral infection that results in heavy yield losses in maize worldwide, particularly in the summer maize-growing regions of china. mrdd is caused by the rice black-streaked dwarf virus (rbsdv). in the present study, analyses of micrornas (mirnas), the degradome, and transcriptome sequences were used to elucidate the rbsdv-responsive pathway(s) in maize. genomic analysis indicated that the expression of three non-conserved and 28 conserved mirnas, representing ... | 2016 | 27493226 |
| epg recordings reveal differential feeding behaviors in sogatella furcifera in response to plant virus infection and transmission success. | plant viruses are primarily transmitted by insect vectors and virus infection may influence on the vectors' feeding behaviors. using an electrical penetration graph, we detected that infection with the southern rice black-streaked dwarf virus (srbsdv) in the white-backed planthopper (wbph) and in rice plants both altered the vector's feeding behavior. when viruliferous wbph (carrying srbsdv) were fed on uninfected plants, they spent more time in salivation and phloem sap ingestion than non-virul ... | 2016 | 27492995 |
| a phloem-limited fijivirus induces the formation of neoplastic phloem tissues that house virus multiplication in the host plant. | a number of phloem-limited viruses induce the development of tumours (enations) in the veins of host plants, but the relevance of tumour induction to the life cycle of those viruses is unclear. in this study, we performed molecular and structural analyses of tumours induced by rice black-streaked dwarf virus (rbsdv, genus fijivirus) infection in maize plants. the transcript level of the maize cdc2 gene, which regulates the cell cycle, was highly elevated in tumour tissues. two-dimensional electr ... | 2016 | 27432466 |
| hairpin rna targeting multiple viral genes confers strong resistance to rice black-streaked dwarf virus. | rice black-streaked dwarf virus (rbsdv) belongs to the genus fijivirus in the family of reoviridae and causes severe yield loss in rice-producing areas in asia. rna silencing, as a natural defence mechanism against plant viruses, has been successfully exploited for engineering virus resistance in plants, including rice. in this study, we generated transgenic rice lines harbouring a hairpin rna (hprna) construct targeting four rbsdv genes, s1, s2, s6 and s10, encoding the rna-dependent rna polyme ... | 2016 | 27187354 |
| identification and characterization of micrornas in the white-backed planthopper, sogatella furcifera. | micrornas (mirnas) are a novel class of small, non-coding endogenous rnas that play critical regulatory roles in many metabolic activities in eukaryotes. reports of the identification of mirnas in sogatella furcifera (white-backed planthopper), the insect that acts as the only confirmed vector of the southern rice black-streaked dwarf virus (srbsdv), are limited. in this study, a total of 382 mirnas were identified in s. furcifera, including 106 conserved and 276 novel mirnas, using high-through ... | 2016 | 27060479 |
| understanding the immune system architecture and transcriptome responses to southern rice black-streaked dwarf virus in sogatella furcifera. | sogatella furcifera, the white-backed planthopper (wbph), has become one of the most destructive pests in rice production owing to its plant sap-sucking behavior and efficient transmission of southern rice black-streaked dwarf virus (srbsdv) in a circulative, propagative and persistent manner. the dynamic and complex srbsdv-wbph-rice plant interaction is still poorly understood. in this study, based on a homology-based genome-wide analysis, 348 immune-related genes belonging to 28 families were ... | 2016 | 27805032 |
| analysis of sogatella furcifera proteome that interact with p10 protein of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) is transmitted efficiently only by white-backed planthopper (wbph, sogatella furcifera) in a persistent propagative manner. here we used a yeast two-hybrid system to investigate the interactions between the srbsdv- p10 and the cdna library of wbph. of 130 proteins identified as putative interactors, 28 were further tested in a retransformation analysis and β-galactosidase assay to confirm the interaction. the full-length gene sequences of 5 candi ... | 2016 | 27653366 |
| maize-pathogen interactions: an ongoing combat from a proteomics perspective. | maize (zea mays l.) is a host to numerous pathogenic species that impose serious diseases to its ear and foliage, negatively affecting the yield and the quality of the maize crop. a considerable amount of research has been carried out to elucidate mechanisms of maize-pathogen interactions with a major goal to identify defense-associated proteins. in this review, we summarize interactions of maize with its agriculturally important pathogens that were assessed at the proteome level. employing diff ... | 2015 | 26633370 |
| small interfering rna pathway modulates initial viral infection in midgut epithelium of insect after ingestion of virus. | numerous viruses are transmitted in a persistent manner by insect vectors. persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. although propagation of viruses in insect vectors can be controlled by the small interfering rna (sirna) antiviral pathway, whether the sirna pathway can control viral dissemination from the midgut epithelium is unknown. infection by a rice virus (southern rice black streaked dwarf vir ... | 2015 | 26537672 |
| [development of transgenic maize with anti-rough dwarf virus artificial mirna vector and their disease resistance]. | maize is one of the most important food crops. rice black-streaked dwarf virus is a maize rough dwarf disease pathogen. the occurrence and transmission of maize rough dwarf disease brings great damage to maize production. the technology of using artificial mirna to build antiviral plant has been proven effective in a variety of plants. however, such trials in maize have not been reported. we designed primers based on the sequence of maize zea-mir159a precursor and sequence of function protein ge ... | 2015 | 26955715 |
| assessment of reference gene stability in rice stripe virus and rice black streaked dwarf virus infection rice by quantitative real-time pcr. | stably expressed reference gene(s) normalization is important for the understanding of gene expression patterns by quantitative real-time pcr (rt-qpcr), particularly for rice stripe virus (rsv) and rice black streaked dwarf virus (rbsdv) that caused seriously damage on rice plants in china and southeast asia. | 2015 | 26497487 |
| relation between the viral load accumulation of southern rice black-streaked dwarf virus and the different developmental stages of sogatella furcifera (hemiptera: delphacidae). | the white-backed planthopper, sogatella furcifera (horvath), is currently the only confirmed vector of southern rice black-streaked dwarf virus (srbsdv), which causes severe rice production losses in china. in this study, an absolute quantification qpcr method was used to detect viral gene mrna expression levels at different developmental stages of white-backed planthoppers fed srbsdv-infected rice plants. a comparison of viral copy numbers of the srbsdv s10 gene at the same developmental stage ... | 2015 | 26470211 |
| identification and expression profiling analysis of calmodulin-binding transcription activator genes in maize (zea mays l.) under abiotic and biotic stresses. | the calmodulin-binding transcription activators (camta) play critical roles in plant growth and responses to environmental stimuli. however, how camtas function in responses to abiotic and biotic stresses in maize (zea mays l.) is largely unknown. in this study, we first identified all the camta homologous genes in the whole genome of maize. the results showed that nine zmcamta genes showed highly diversified gene structures and tissue-specific expression patterns. many zmcamta genes displayed h ... | 2015 | 26284092 |
| genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice. | rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ... | 2015 | 26198760 |
| whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper. | rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ... | 2015 | 26149652 |
| molecular genetic analysis and evolution of segment 7 in rice black-streaked dwarf virus in china. | rice black-streaked dwarf virus (rbsdv) causes maize rough dwarf disease or rice black-streaked dwarf disease and can lead to severe yield losses in maize and rice. to analyse rbsdv evolution, codon usage bias and genetic structure were investigated in 111 maize and rice rbsdv isolates from eight geographic locations in 2013 and 2014. the linear dsrna s7 is a+u rich, with overall codon usage biased toward codons ending with a (a3s, s7-1: 32.64%, s7-2: 29.95%) or u (u3s, s7-1: 44.18%, s7-2: 46.06 ... | 2015 | 26121638 |
| quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. | southern rice black streaked dwarf virus (srbsdv) and rice black streaked dwarf virus (rbsdv) are serious rice-infecting reoviruses, which are transmitted by different planthoppers in a persistent propagative manner. in this study, we quantitatively compared the spatial distribution of srbsdv and rbsdv contents over time in their vector and nonvector insects using real time-pcr. genome equivalent copies (gec) were assessed every 2 days from 0 to 14 days after a 3-days acquisition access period ( ... | 2015 | 26116274 |
| studies of binding interactions between dufulin and southern rice black-streaked dwarf virus p9-1. | southern rice black-streaked dwarf virus (srbsdv) is an important rice virus that can cause considerable crop losses. during infection, srbsdv p9-1 accumulates viroplasms in srbsdv-infected plant and insect cells, which may facilitate the formation of srbsdv particles. our field trial results showed that dufulin inhibited infection activity in srbsdv-infected rice. however it is not clear how dufulin inhibits the infection activity. therefore we studied the interactions between dufulin and srbsd ... | 2015 | 25934228 |
| characterization of the importance of terminal residues for southern rice black-streaked dwarf virus p9-1 viroplasm formations. | southern rice black-streaked dwarf virus (srbsdv) p9-1 is involved in viroplasm formations in the srbsdv-infected plants and insects. during infection, srbsdv p9-1 is an important protein. however, the function characterization of p9-1 octamers in vitro and in yeast is still obscure. in this study, the secondary and 3d structure of srbsdv p9-1 was predicted, then srbsdv p9-1 was expressed and analyzed in vitro, a size exclusion chromatography assay showed that p9-1 had the ability to form octame ... | 2015 | 25870931 |
| quantitative analysis of southern rice black-streaked dwarf virus in sogatella furcifera and virus threshold for transmission. | we investigated southern rice black-streaked dwarf virus (srbsdv) accumulation in a vector insect, the whitebacked planthopper (sogatella furcifera), to elucidate the association of virus accumulation in the vector with virus transmission efficiency. real-time quantitative reverse transcription polymerase chain reaction analysis confirmed that this virus is transmitted in a persistent-propagative manner. srbsdv was successfully transmitted by s. furcifera males in which rna accumulation of the c ... | 2015 | 25870927 |
| presence of poly(a) tails at the 3'-termini of some mrnas of a double-stranded rna virus, southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv), a new member of the genus fijivirus, is a double-stranded rna virus known to lack poly(a) tails. we now showed that some of srbsdv mrnas were indeed polyadenylated at the 3' terminus in plant hosts, and investigated the nature of 3' poly(a) tails. the non-abundant presence of srbsdv mrnas bearing polyadenylate tails suggested that these viral rna were subjected to polyadenylation-stimulated degradation. the discovery of poly(a) tails in differen ... | 2015 | 25835534 |
| interaction research on the antiviral molecule dufulin targeting on southern rice black streaked dwarf virus p9-1 nonstructural protein. | southern rice black streaked dwarf virus (srbsdv) causes severe harm to rice production. unfortunately, studies on effective antiviral drugs against srbsdv and interaction mechanism of antiviral molecule targeting on srbsdv have not been reported. this study found dufulin (dfl), an ideal anti-srbsdv molecule, and investigated the interactions of dfl targeting on the nonstructural protein p9-1. the biological sequence information and bonding characterization of dfl to four kinds of p9-1 protein w ... | 2015 | 25807053 |
| p5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts. | the genome segment s5 of rice black-streaked dwarf virus (genus fijivirus, family reoviridae) is functionally bicistronic in infected plants. it has a conserved second orf (p5-2) partially overlapping the major orf in a different reading frame, but its function remains unknown. p5-2 was detected in infected plants, but not in purified viral particles by western blotting, indicating that it is a non-structural protein. in immunoelectron microscopy, polyclonal antibodies against p5-2 specifically ... | 2015 | 25749897 |
| first discovery of acetone extract from cottonseed oil sludge as a novel antiviral agent against plant viruses. | a novel acetone extract from cottonseed oil sludge was firstly discovered against plant viruses including tobacco mosaic virus (tmv), rice stripe virus (rsv) and southern rice black streaked dwarf virus (srbsdv). gossypol and β-sitosterol separated from the acetone extract were tested for their effects on anti-tmv and analysed by nuclear magnetic resonance (nmr) assay. in vivo and field trials in different geographic distributions and different host varieties declared that this extract mixture w ... | 2015 | 25705894 |
| nac transcription factor family genes are differentially expressed in rice during infections with rice dwarf virus, rice black-streaked dwarf virus, rice grassy stunt virus, rice ragged stunt virus, and rice transitory yellowing virus. | expression levels of the nac gene family were studied in rice infected with rice dwarf virus (rdv), rice black-streaked dwarf virus (rbsdv), rice grassy stunt virus (rgsv), rice ragged stunt virus (rrsv), and rice transitory yellowing virus (rtyv). microarray analysis showed that 75 (68%) osnac genes were differentially regulated during infection with rdv, rbsdv, rgsv, and rrsv compared with the control. the number of osnac genes up-regulated was highest during rgsv infection, while the lowest n ... | 2015 | 26442000 |
| phylogenetic and recombination analysis of rice black-streaked dwarf virus segment 9 in china. | rice black-streaked dwarf virus (rbsdv) is an economically important virus that causes maize rough dwarf disease and rice black-streaked dwarf disease in east asia. to study rbsdv variation and recombination, we examined the segment 9 (s9) sequences of 49 rbsdv isolates from maize and rice in china. three s9 recombinants were detected in baoding, jinan, and jining, china. phylogenetic analysis showed that chinese rbsdv isolates could be classified into two groups based on their s9 sequences, reg ... | 2015 | 25633210 |
| genome-wide characterization of rice black streaked dwarf virus-responsive micrornas in rice leaves and roots by small rna and degradome sequencing. | micrornas (mirnas) are small, non-coding rnas which typically function by guiding cleavage of target mrnas. they play important roles in development, abiotic stress and responses to pathogens. four small rna libraries and four degradome libraries were constructed from the leaves and roots of healthy rice and plants infected with rice black streaked dwarf virus (rbsdv). analysis of the deep sequencing results showed that the expression patterns of 14 mirnas in leaves and 16 mirnas in roots change ... | 2015 | 25535197 |
| a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector. | rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ... | 2015 | 25455902 |
| characterization of homologous and heterologous interactions between viroplasm proteins p6 and p9-1 of the fijivirus southern rice black-streaked dwarf virus. | p6 of southern rice black-streaked dwarf virus (srbsdv) is a multifunctional protein that is involved in the formation of viroplasms by interacting with p5-1. here, we used yeast two-hybrid and bimolecular fluorescence complementation assays to show that there were homologous and heterologous interactions between srbsdv p6 and p9-1 in yeast and plant cells. mutational analysis showed that the n-terminal region (residues 1-93) of p6 was necessary for the interaction between p6 and p9-1. self-inte ... | 2015 | 25377635 |
| infection of rice plants by rice black streaked dwarf virus improves an egg parasitoid, anagrus nilaparvatae (hymenoptera: mymaridae), of rice planthoppers. | the effects of rice plants infected by rice black streaked dwarf virus (rbsdv) on the host preference, duration of immature stages, sex ratio, and adult longevity and parasitic capacity of an egg parasitoid, anagrus nilaparvatae pang et wang, of rice brown planthopper, nilaparvata lugens stål, were evaluated. tests of response to plant volatiles using an olfactometer showed that a. nilaparvatae preferred rice plants harboring rice brown planthopper eggs over plants free of rice brown planthopper ... | 2014 | 25199055 |
| virus-mediated chemical changes in rice plants impact the relationship between non-vector planthopper nilaparvata lugens stål and its egg parasitoid anagrus nilaparvatae pang et wang. | in order to clarify the impacts of southern rice black-streaked dwarf virus (srbsdv) infection on rice plants, rice planthoppers and natural enemies, differences in nutrients and volatile secondary metabolites between infected and healthy rice plants were examined. furthermore, the impacts of virus-mediated changes in plants on the population growth of non-vector brown planthopper (bph), nilaparvata lugens, and the selectivity and parasitic capability of planthopper egg parasitoid anagrus nilapa ... | 2014 | 25141278 |
| a novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host. | rice black-streaked dwarf virus (rbsdv) naturally infects gramineae plants through small brown planthopper (sbph) as a vector. however, rbsdv cannot be transmitted to the sbph offspring through transovarian transmission. wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of rbsdv in farm ecosystem. we developed a novel, in vivo, indoor method to preserve rbsdv in sbph using wheat seedling as a bridge host. the temperature range of 23-27°c w ... | 2014 | 25075933 |
| micrornas responding to southern rice black-streaked dwarf virus infection and their target genes associated with symptom development in rice. | southern rice black-streaked dwarf virus (srbsdv) is a recently emerged rice virus that has spread across asia. this devastating virus causes rice plants to produce a variety of symptoms during different growth stages. micrornas (mirnas) comprise a large group of 21-24-nt rna molecules that are important regulators of plant development processes and stress responses. in this study, we used microarray profiling to investigate rice mirnas responding to srbsdv infection at 3, 9, 15, and 20 days pos ... | 2014 | 25038403 |
| tumours induced by a plant virus are derived from vascular tissue and have multiple intercellular gateways that facilitate virus movement. | structural studies showed that tumours induced by southern rice black-streaked dwarf virus (srbsdv; genus fijivirus, family reoviridae) were highly organized, modified phloem, composed of sclerenchyma, vessels, hyperplastic phloem parenchyma and sieve elements (ses). only parenchyma and ses were invaded by the virus. there was a special region that consisted exclusively of ses without the usual companion cells and a new flexible type of intercellular gateway was observed on all se-se interfaces ... | 2014 | 24987015 |
| virus-induced tubule: a vehicle for rapid spread of virions through basal lamina from midgut epithelium in the insect vector. | the plant reoviruses, plant rhabdoviruses, tospoviruses, and tenuiviruses are transmitted by insect vectors in a persistent propagative manner. these viruses induce the formation of viral inclusions to facilitate viral propagation in insect vectors. the intestines of insect vectors are formed by epithelial cells that lie on the noncellular basal lamina surrounded by visceral muscle tissue. here, we demonstrate that a recently identified plant reovirus, southern rice black-streaked dwarf virus (s ... | 2014 | 24965461 |
| synergism between southern rice black-streaked dwarf virus and rice ragged stunt virus enhances their insect vector acquisition. | southern rice black-streaked dwarf virus (srbsdv), a tentative species in the genus fijivirus, family reoviridae, is a novel rice virus transmitted by the white-backed planthopper (sogatella furcifera). since its discovery in 2001, srbsdv has spread rapidly throughout eastern and southeastern asia and caused large rice losses in china and vietnam. rice ragged stunt virus (rrsv) (genus oryzavirus, family reoviridae) is a common rice virus vectored by the brown planthopper (nilaparvata lugens). rr ... | 2014 | 24915431 |
| southern rice black-streaked dwarf virus infection improves host suitability for its insect vector, sogatella furcifera (hemiptera: delphacidae). | the impact of southern rice black-streaked dwarf virus (srbsdv) on the life cycle of the vector sogatella furcifera (horváth) (hemiptera: delphacidae) was investigated. the adult longevity was significantly shorter when the insect was reared on the srbsdv-infected rice plants than those reared on the noninfected rice treatment. the fecundity of s. furcifera reared on the srbsdv-infected rice plants was slightly higher than that of those reared on the noninfected rice plants both in macropterous ... | 2014 | 24665689 |
| southern rice black-streaked dwarf virus (srbsdv) directly affects the feeding and reproduction behavior of its vector, sogatella furcifera (horváth) (hemiptera: delphacidae). | southern rice black-streaked dwarf virus (srbsdv) is a recently discovered member of the genus fijivirus and it is transmitted by the rice whitebacked planthopper (wbph), sogatella furcifera (horváth). it was found that srbsdv infected vectors might contribute negatively to the wbph population, although the longer nymphal period might benefit viral acquisition, transmission and increase infection rate. the interaction between srbsdv and its vector need to be further explored to gain better under ... | 2014 | 24661747 |
| proteomic analysis of interaction between p7-1 of southern rice black-streaked dwarf virus and the insect vector reveals diverse insect proteins involved in successful transmission. | southern rice black-streaked dwarf virus (srbsdv), transmitted by the white-backed planthopper (sogatella furcifera) in a persistent-propagative manner, has caused serious yield losses in asia. here in a yeast two-hybrid system, protein interactions between srbsdv p7-1 as a bait protein and a cdna library of s. furcifera as prey protein were assessed. of 153 proteins identified as putative interactors, 24 were selected for further analysis. of the 24 proteins, 18 were further confirmed in a chem ... | 2014 | 24650428 |
| southern rice black-streaked dwarf virus alters insect vectors' host orientation preferences to enhance spread and increase rice ragged stunt virus co-infection. | in recent years, southern rice black-streaked dwarf virus (srbsdv), a tentative species in the genus fijivirus (family reoviridae), has spread rapidly and caused serious rice losses in eastern and southeastern asia. with this virus spread, rice ragged stunt virus (rrsv, genus oryzavirus, family reoviridae) became more common in southern china, usually in co-infection with the former. srbsdv and rrsv are transmitted by two different species of planthoppers, white-backed planthopper (wbph, sogatel ... | 2014 | 24047253 |
| rice black-streaked dwarf virus genome segment s5 is a bicistronic mrna in infected plants. | rice black-streaked dwarf virus (rbsdv) is a recognized member of the genus fijivirus, family reoviridae. genome segment s5 has a putative second orf partially overlapping the major orf but in a different reading frame. this putative orf is present in a published sequence and in two chinese isolates now sequenced. antibodies were raised against purified p5-1 and p5-2 fusion proteins expressed in a prokaryotic system. in western blots, these antibodies reacted with proteins of about 106 and 27 kd ... | 2014 | 24013236 |
| influence of rice black streaked dwarf virus on the ecological fitness of non-vector planthopper nilaparvata lugens (hemiptera: delphacidae). | rice black streak dwarf virus (rbsdv) is transmitted by the small brown planthopper (sbph), laodelphax striatellus (fallen). non-vector rice brown planthopper (bph), nilaparvata lugens (stål), shares the same host rice plants with sbph in paddy fields. the changes in nutritional composition of rice plants infected by rbsdv and the ecological fitness of bph feeding on the infected plants were studied under both artificial climate chamber and field conditions. contents of 16 detected amino acids a ... | 2014 | 23956237 |
| interactive effects of southern rice black-streaked dwarf virus infection of host plant and vector on performance of the vector, sogatella furcifera (homoptera: delphacidae). | performance of insect vectors can be influenced by the viruses they transmit, either directly by infection of the vectors or indirectly via infection of the host plants. southern rice black-streaked dwarf virus (srbsdv) is a propagative virus transmitted by the white-backed planthopper, sogatella furcifera (hovath). to elucidate the influence of srbsdv on the performance of white-backed planthopper, life parameters of viruliferous and nonviruliferous white-backed planthopper fed rice seedlings i ... | 2014 | 26309259 |
| development of proteomic technology of shotgun and label free combined with multiple reaction monitoring to simultaneously detect southern rice black-streaked dwarf virus and rice ragged stunt virus. | the co-infection of rice caused by southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) was widely found at many regions, such as yunnan province, china, and north and central vietnam. these rice viral diseases lead to seriously yield loss of rice. in this study, the proteomics technology of shotgun and label free combined with multiple reaction monitoring (mrm) was developed to detect rice sample of a single or/and co-infection. the shotgun assay indicated that s ... | 2014 | 25674599 |
| identification and profiling of conserved and novel micrornas in laodelphax striatellus in response to rice black-streaked dwarf virus (rbsdv) infection. | micrornas (mirnas) are small non-coding endogenous rna molecules that play important roles in various biological processes. this study examined microrna profiles of laodelphax striatellus using the small rna libraries derived from virus free (vf) and rice black-streaked dwarf virus (rbsdv) infected (rb) insects. a total of 59 mature mirnas (46 mirna families) were identified as conserved insect mirnas in both vf and rb libraries. among these conserved mirnas, 24 were derived from the two arms of ... | 2014 | 26484150 |
| selection of reference genes for gene expression studies in virus-infected monocots using quantitative real-time pcr. | both genome-wide transcriptomic surveys of the mrna expression profiles and virus-induced gene silencing-based molecular studies of target gene during virus-plant interaction involve the precise estimation of the transcript abundance. quantitative real-time pcr (qpcr) is the most widely adopted technique for mrna quantification. in order to obtain reliable quantification of transcripts, identification of the best reference genes forms the basis of the preliminary work. nevertheless, the stabilit ... | 2013 | 23954326 |
| simultaneous detection and differentiation of rice black streaked dwarf virus (rbsdv) and southern rice black streaked dwarf virus (srbsdv) by duplex real time rt-pcr. | the diseases caused by rice black streaked dwarf virus (rbsdv) and southern rice black streaked dwarf virus (srbsdv) have been occurring epidemically in china and southeastern asia in recent years. a sensitive, reliable and quantitative method is required to detect and distinguish for rbsdv and srbsdv in rice and vector insects. | 2013 | 23331990 |
| enhanced virus resistance in transgenic maize expressing a dsrna-specific endoribonuclease gene from e. coli. | maize rough dwarf disease (mrdd), caused by several fijiviruses in the family reoviridae, is a global disease that is responsible for substantial yield losses in maize. although some maize germplasm have low levels of polygenic resistance to mrdd, highly resistant cultivated varieties are not available for agronomic field production in china. in this work, we have generated transgenic maize lines that constitutively express rnc70, a mutant e. coli dsrna-specific endoribonuclease gene. transgenic ... | 2013 | 23593318 |
| identification and fine-mapping of a qtl, qmrdd1, that confers recessive resistance to maize rough dwarf disease. | maize rough dwarf disease (mrdd) is a devastating viral disease that results in considerable yield losses worldwide. three major strains of virus cause mrdd, including maize rough dwarf virus in europe, mal de río cuarto virus in south america, and rice black-streaked dwarf virus in east asia. these viral pathogens belong to the genus fijivirus in the family reoviridae. resistance against mrdd is a complex trait that involves a number of quantitative trait loci (qtl). the primary approach used t ... | 2013 | 24079304 |
| screening anti-southern rice black-streaked dwarf virus drugs based on s7-1 gene expression in rice suspension cells. | southern rice black-streaked dwarf virus (srbsdv) is a rice pathogen that had an outbreak in southern china in 2010 and caused significant crop losses. therefore, screening for effective antiviral drugs against srbsdv is very important. this study used rice suspension cells infected with srbsdv by polyethylene glycol-mediated uptake for screening antiviral drugs. srbsdv p7-1, which is coded by the s7-1 gene, has an intrinsic ability to self-interact to form tubules that play an important role in ... | 2013 | 23915352 |
| one-step multiplex reverse transcription-polymerase chain reaction for the simultaneous detection of three rice viruses. | rice stripe virus (rsv), rice black-streaked dwarf virus (rbsdv), and rice dwarf virus (rdv) are major rice-infecting viruses in korea that can cause serious crop losses. a one-step multiplex reverse transcription-polymerase chain reaction (mrt-pcr) was developed for the simultaneous detection of these rice viruses. three sets of specific primers targeted to the capsid protein coding genes of rsv, rbsdv, and rdv were used to amplify fragments that were 703 bp, 485 bp, and 252 bp, respectively. t ... | 2013 | 23850700 |
| genetic structure of rice black-streaked dwarf virus populations in china. | rice black-streaked dwarf virus (rbsdv) is a double-stranded rna (dsrna) virus belonging to the genus fijivirus in the family reoviridae. the genome of rbsdv consists of ten dsrna segments. although rbsdv has caused significant economic losses to rice and maize production in the past few years in china, its molecular diversity and evolution remain largely unknown. to elucidate the factor(s) underlying the evolution of rbsdv, we determined segment 8 (s8; carrying orf8 encoding the minor core caps ... | 2013 | 23807744 |
| characterization of rice black-streaked dwarf virus- and rice stripe virus-derived sirnas in singly and doubly infected insect vector laodelphax striatellus. | replication of rna viruses in insect cells triggers an antiviral defense that is mediated by rna interference (rnai) which generates viral-derived small interfering rnas (sirnas). however, it is not known whether an antiviral rnai response is also induced in insects by reoviruses, whose double-stranded rna genome replication is thought to occur within core particles. deep sequencing of small rnas showed that when the small brown planthopper (laodelphax striatellus) was infected by rice black-str ... | 2013 | 23776591 |
| effects of southern rice black-streaked dwarf virus on the development and fecundity of its vector, sogatella furcifera. | southern rice black-streaked dwarf virus (srbsdv) threatens rice production in china and vietnam. the virus is vectored by the migrating white-backed planthopper (wbph, sogatella furcifera) in a circulative, propagative, and persistent manner. a persistently-transmitted plant virus might affect its vector's development and fecundity directly by infecting the vector itself and/or indirectly altering the host plant. this study evaluated the direct and indirect effects of srbsdv on wbph performance ... | 2013 | 23663428 |
| non-structural protein p6 encoded by rice black-streaked dwarf virus is recruited to viral inclusion bodies by binding to the viroplasm matrix protein p9-1. | like other members of the family reoviridae, rice black-streaked dwarf virus (rbsdv, genus fijivirus) is thought to replicate and assemble within cytoplasmic viral inclusion bodies, commonly called viroplasms. rbsdv p9-1 is the key protein for the formation of viroplasms, but little is known about the other proteins of the viroplasm or the molecular interactions amongst its components. rbsdv non-structural proteins were screened for their association with p9-1 using a co-immunoprecipitation assa ... | 2013 | 23636822 |
| quantitative detection of relative expression levels of the whole genome of southern rice black-streaked dwarf virus and its replication in different hosts. | in recent years, a disease caused by southern rice black-streaked dwarf virus (srbsdv) has resulted in significant loss in rice production in southern china and has spread quickly throughout east and southeast asia. this virus is transmitted by an insect vector, white-backed planthopper (wbph) sogatella furcifera (hemiptera: delphacidae), in a persistent propagative manner. aside from rice, srbsdv can also infect numerous poaceae plants. however, the molecular mechanism of interaction between sr ... | 2013 | 23631705 |
| evidence of recombination and genetic diversity in southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) causes one of the most serious viral diseases of rice in china and vietnam. sequence identities of s10, encoding the major capsid protein, were 98.0 %-100 % and 98.3 %-100 % at the nucleotide and amino acid level, respectively. our results suggest that the codon at position 550 of s10 is under positive selection, while most of the other codons are under neutral evolution. putative recombination events were identified in genomic rna segments s1, 2 ... | 2013 | 23605668 |
| identification and characterization of the interaction between viroplasm-associated proteins from two different plant-infecting reoviruses and eef-1a of rice. | a rice protein homologous to eukaryotic translation elongation factor 1a (eef-1a) was found to interact with the pns6 of rice ragged stunt virus (rrsv), the type member of the genus oryzavirus, family reoviridae, in yeast two-hybrid screening. the interaction between the rice protein, designated oseef-1a, and rrsv pns6 was confirmed by bimolecular fluorescence complementation. besides pns6, oseef-1a also interacted with the viroplasm matrix protein, pns10, of rrsv. when expressed together, oseef ... | 2013 | 23605590 |
| new model for the genesis and maturation of viroplasms induced by fijiviruses in insect vector cells. | plant reoviruses are thought to replicate and assemble within cytoplasmic, nonmembranous structures called viroplasms. here, we established continuous cell cultures of the white-backed planthopper (sogatella furcifera horváth) to investigate the mechanisms for the genesis and maturation of the viroplasm induced by southern rice black-streaked dwarf virus (srbsdv), a fijivirus in the family reoviridae, during infection of its insect vector. electron and confocal microscopy revealed that the virop ... | 2013 | 23576499 |
| development and use of three monoclonal antibodies for the detection of rice black-streaked dwarf virus in field plants and planthopper vectors. | rice black-streaked dwarf virus (rbsdv) causes great losses in rice, maize and wheat production in asian countries. the use of serological methods for rbsdv detection depends on the availability of antibodies. in this study, three highly sensitive and specific murine monoclonal antibodies (mabs) against rbsdv antigens were produced using crude extracts from tumors of rbsdv-infected maize as the immunogen, and two serological assays, antigen-coated-plate enzyme-linked immunosorbent assay (acp-eli ... | 2013 | 23575411 |
| interactions between the p6 and p5-1 proteins of southern rice black-streaked dwarf fijivirus in yeast and plant cells. | southern rice black-streaked dwarf virus (srbsdv) is a recently described member of the genus fijivirus, family reoviridae. the roles of the proteins encoded by the srbsdv genome have rarely been studied. in a yeast two-hybrid (yth) assay in which srbsdv p6, a putatively multifunctional protein, was used as bait and an srbsdv cdna library was used as prey, there was a strong interaction between the p6 and p5-1 proteins. the interaction was confirmed by bimolecular fluorescence complement (bifc) ... | 2013 | 23474918 |
| dynamics of southern rice black-streaked dwarf virus in rice and implication for virus acquisition. | a novel viral disease of rice caused by southern rice black-streaked dwarf virus (srbsdv) has spread throughout east and southeast asia since the mid-2000s. outbreaks of this viral disease occur yearly in southern parts of japan concurrently with overseas migration of the planthopper vector sogatella furcifera from southern china during the rainy season (from late june to early july). we examined the dynamics (changes in titer and localization) of srbsdv on rice using reverse-transcription real- ... | 2013 | 23301813 |
| comparative proteomic analysis reveals the cross-talk between the responses induced by h2o2 and by long-term rice black-streaked dwarf virus infection in rice. | hydrogen peroxide (h2o2) could be produced during the plant-virus compatible interaction. however, the cell responses regulated by the enhanced h2o2 in virus infected plant are largely unknown. to make clear the influence of rice black-streaked dwarf virus (rbsdv) infection on h2o2 accumulation, we measured the content of h2o2 and found the h2o2 level was increased in rice seedlings inoculated with rbsdv. to reveal the responses initiated by the enhanced h2o2 during plant-virus interaction, the ... | 2013 | 24312331 |
| migration of rice planthoppers and their vectored re-emerging and novel rice viruses in east asia. | this review examines recent studies of the migration of three rice planthoppers, laodelphax striatellus, sogatella furcifera, and nilaparvata lugens, in east asia. laodelphax striatellus has recently broken out in jiangsu province, eastern china. the population density in the province started to increase in the early 2000s and peaked in 2004. in 2005, rice stripe virus (rsv) viruliferous rate of l. striatellus peaked at 31.3%. since then, rice stripe disease spread severely across the whole prov ... | 2013 | 24312081 |
| overexpression of rice black-streaked dwarf virus p7-1 in arabidopsis results in male sterility due to non-dehiscent anthers. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, is propagatively transmitted by the small brown planthopper (laodelphax striatellus fallén). rbsdv causes rice black-streaked dwarf and maize rough dwarf diseases, which lead to severe yield losses in crops in china. although several rbsdv proteins have been studied in detail, the functions of the nonstructural protein p7-1 are still largely unknown. to investigate the role of the p7-1 protein in v ... | 2013 | 24260239 |
| rice black-streaked dwarf virus p10 induces membranous structures at the er and elicits the unfolded protein response in nicotiana benthamiana. | endoplasmic reticular (er) membrane modifications play an important role in viral rna replication and virion assembly but little is known about the involvement of er-membrane remodeling in the infection cycle of fijiviruses in plant cells. the subcellular localization of rice black-streaked dwarf virus outer capsid p10 was therefore examined using live-cell imaging. p10 fused to egfp formed vesicular structures associated with er membranes in nicotiana benthamiana epidermal cells and in rice pro ... | 2013 | 24210107 |
| nonstructural protein p7-2 encoded by rice black-streaked dwarf virus interacts with skp1, a core subunit of scf ubiquitin ligase. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus within the family reoviridae, causes severe damage to cereal crops in south east asia. the protein p7-2, encoded by the second open reading frame of segment s7, is conserved among most plant-infecting fijiviruses, but its function is still obscure. | 2013 | 24176102 |
| distribution and genetic diversity of southern rice black-streaked dwarf virus in china. | rice and maize dwarf diseases caused by the newly introduced southern rice black-streaked dwarf virus (srbsdv) have led to severe economic losses in south china in recent years. the distribution and diversity of srbsdv have not been investigated in the main rice and maize growing areas in china. in this study, the distribution of srbsdv in china was determined by using reverse transcription-polymerase chain reaction (rt-pcr). | 2013 | 24131521 |
| viroplasm protein p9-1 of rice black-streaked dwarf virus preferentially binds to single-stranded rna in its octamer form, and the central interior structure formed by this octamer constitutes the major rna binding site. | the p9-1 protein of rice black-streaked dwarf virus (rbsdv) is an essential part of the viroplasm. however, little is known about its nature or biological function in the viroplasm. in this study, the structure and function of p9-1 were analyzed for in vitro binding to nucleic acids. we found that the p9-1 protein preferentially bound to single-stranded versus double-stranded nucleic acids; however, the protein displayed no preference for rbsdv versus non-rbsdv single-stranded ssrna (ssrna). a g ... | 2013 | 24067964 |
| southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in asia. | southern rice black-streaked dwarf virus (srbsdv), a non-enveloped icosahedral virus with a genome of 10 double-stranded rna segments, is a novel species in the genus fijivirus (family reoviridae) first recognized in 2008. rice plants infected with this virus exhibit symptoms similar to those caused by rice black-streaked dwarf virus. since 2009, the virus has rapidly spread and caused serious rice losses in east and southeast asia. significant progress has been made in recent years in understan ... | 2013 | 24058362 |
| methodology for antibody preparation and detection of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) is a member of the genus fijivirus of the family reoviridae that causes a devastating disease in rice. seven polypeptide fragments of the putative uncharacterized protein (pup) and the p10 proteins of srbsdv were designed, synthesized, and used to immunize rabbits. titers of polyclonal antibodies against the seven peptides were examined using indirect enzyme-linked immunosorbent assay (elisa), and their specificities were investigated using weste ... | 2012 | 22899314 |
| restriction of viral dissemination from the midgut determines incompetence of small brown planthopper as a vector of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv), a fijivirus, is transmitted by the white-backed planthopper in a persistent-propagative manner. in this study, we found that another planthopper species, the small brown planthopper (sbph), could acquire srbsdv but not transmit it. to identify the transmission barrier for srbsdv in sbphs, sequential infection by srbsdv in the organs of sbphs was studied with immunofluorescence for viral antigens. srbsdv initially entered the epithelial cells of ... | 2012 | 22683297 |
| dot immunobinding assay method with chlorophyll removal for the detection of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv), a new virus from fiji, has seriously damaged rice crops in southern china and northern vietnam in recent years. this virus is difficult to diagnose in the early stages of infection, and is very destructive at the late stage. in the present study, a dot immunobinding assay (diba) that has a high sensitivity for diagnosing srbsdv was developed. two kinds of treatment for the diba were evaluated to determine the most effective one for removing chlo ... | 2012 | 22669043 |
| mapping quantitative trait loci conferring resistance to rice black-streaked virus in maize (zea mays l.). | maize rough dwarf disease (mrdd) is one of the most serious virus diseases of maize worldwide, and it causes great reduction of maize production. in china, the pathogen was shown to be rice black-streaked virus (rbsdv). currently, mrdd has spread broadly and leads to significant loss in china. however, there has been little research devoted to this disease. our aims were to identify the markers and loci underlying resistance to this virus disease. in this study, segregation populations were cons ... | 2012 | 22562145 |