Publications
Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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multiplex pcr and 12s rrna gene sequencing for detection of meat adulteration: a case study in the egyptian markets. | recently, dna-based methods have proved to be accurate, fast and sensitive for meat authentication. according to the european union, the food safety standards require accurate and detailed composition information of the meat products. therefore, an accurate, fast and cost-effective identification methodology is needed. in this study, multiplex pcr coupled with 12s rdna sequencing was employed for the detection of meat adulteration in two red meat products (frozen beef liver and cold cut samples, ... | 2021 | 32860900 |
tissue expression profile, polymorphism of igf1 gene and its effect on body size traits of dezhou donkey. | insulin-like growth factor 1 (igf1) is a multifunctional cell proliferation regulator that plays a critical role in regulating animal growth and development. in this study, the expression level of igf1 gene in different tissues of dezhou donkey in different periods was investigated by rt-qpcr. meanwhile, two mutation sites were identified within the igf1 gene and its effect on body size traits of dezhou donkey was analysed. the results showed that the expression level of the adult donkey igf1 ge ... | 2021 | 32896588 |
response of uk horse, pony and donkey owners to the early stages of the covid-19 pandemic. | in the uk, march 2020 was a time of great uncertainty as covid-19 became increasingly widespread. the government responded by making suggestions about how people could reduce the risk of spread on 16 march, moved swiftly into closing schools on the 18 march before announcing a mandatory lockdown on the 23rd march. this was a challenging time for uk equestrians who had to balance maintaining their equine's routine and daily care alongside the increasing biosecurity measures. a cross-sectional sur ... | 2021 | 33922408 |
a hepatitis b virus causes chronic infections in equids worldwide. | preclinical testing of novel therapeutics for chronic hepatitis b (chb) requires suitable animal models. equids host homologs of hepatitis c virus (hcv). because coinfections of hepatitis b virus (hbv) and hcv occur in humans, we screened 2,917 specimens from equids from five continents for hbv. we discovered a distinct hbv species (equid hbv, eqhbv) in 3.2% of donkeys and zebras by pcr and antibodies against eqhbv in 5.4% of donkeys and zebras. molecular, histopathological, and biochemical anal ... | 2021 | 33723007 |
a scoping review of west nile virus seroprevalence studies among african equids. | west nile virus (wnv) is an emerging and re-emerging zoonotic flavivirus first identified in and endemic to africa. the virus is transmitted between birds by biting mosquitoes, with equids and humans being incidental hosts. the majority of infected incidental hosts display no or only mild clinical signs, but a fraction develop encephalitis. the aim of this scoping review was to identify and evaluate primary research on the presence of antibodies to wnv among african equids. three bibliographic d ... | 2021 | 34358049 |
west nile virus seroprevalence in a selected donkey population of namibia. | west nile virus (wnv) is a mosquito-borne virus enzootically maintained in birds. however, it can incidentally infect other species, leading to sometimes severe clinical consequences like in horses and especially human beings. despite the topic relevance, the presence and distribution of wnv are currently unknown in namibia. several countries implement surveillance systems based on virus detection in birds, mosquitoes, and vertebrate species including horses. the present study aimed to fill this ... | 2021 | 34222404 |
active equine parvovirus-hepatitis infection is most frequently detected in austrian horses of advanced age. | equine parvovirus-hepatitis (eqpv-h) research is in its infancy. information regarding prevalence, geographical distribution, genetic diversity, pathogenesis and risk factors enhances understanding of this potentially fatal infection. | 2021 | 33704819 |
epidemiology and serological detection of rift valley fever disease in farm animals in southern egypt. | in this study, the serological surveillance of rift valley fever virus (rvfv) in southern egypt was carried out for 460 serum samples collected from domestic animals (unvaccinated), including cattle, sheep, goat, camel and donkey reared in three different provinces (qena, luxor and aswan). enzyme linked immunosorbent assay (elisa) was used to detect rvfv antibodies. the results showed that 97 out of 460 animals were positive by using blocking elisa. the percentage of rvfv infection in cattle, sh ... | 2021 | 33567844 |
a reassortant g3p12 rotavirus a strain associated with severe enteritis in donkeys (equus asinus). | in contrast to horses, the only evidence suggesting gastrointestinal disease in neonatal donkeys is associated with group a rotaviruses (rvas) is the detection of viral antigens by elisa in just 1 of 82 symptomatic donkey foals. no additional, more comprehensive investigations have been conducted, and rvas if circulating in donkey populations have not been molecularly characterised. | 2021 | 33455000 |
stabilization of a broadly neutralizing anti-chikungunya virus single domain antibody. | a single domain antibody (clone cc3) previously found to neutralize a vaccine strain of the chikungunya virus (prnt50 = 2. 5 ng/ml) was found to be broadly neutralizing. clone cc3 is not only able to neutralize a wild-type (wt) strain of chikungunya virus (chikv), but also neutralizes wt strains of mayaro virus (mayv) and ross river virus (rrv); both arthralgic, old world alphaviruses. interestingly, cc3 also demonstrated a degree of neutralizing activity against the new world alphavirus, venezu ... | 2021 | 33585527 |
a monovalent and trivalent mva-based vaccine completely protects mice against lethal venezuelan, western, and eastern equine encephalitis virus aerosol challenge. | venezuelan, eastern and western equine encephalitis viruses (eev) can cause severe disease of the central nervous system in humans, potentially leading to permanent damage or death. yet, no licensed vaccine for human use is available to protect against these mosquito-borne pathogens, which can be aerosolized and therefore pose a bioterror threat in addition to the risk of natural outbreaks. using the mouse aerosol challenge model, we evaluated the immunogenicity and efficacy of eev vaccines that ... | 2021 | 33542715 |
evaluation of colostral immunity against equine herpesvirus type 1 (ehv-1) in martina franca's foals. | eight martina franca pregnant jennies were selected in order to evaluate the transfer of colostral antibodies against equine herpesvirus type 1 in their relative foals after immunization with a commercial inactivated vaccine, compared with an unvaccinated group. samples of serum and colostrums/milk were collected from jennies and foals under study starting from 10 min before and up to 21 days after the foaling. specific anti-ehv-1 antibody titers were evaluated by means of a serum neutralization ... | 2020 | 33330699 |
in vitro evaluation of probiotic potential and safety assessment of lactobacillus mucosae strains isolated from donkey's lactation. | the study, for the first time, reports the efficacy, safety and probiotic properties of two lactobacillus mucosae strains, lact. mucosae srv5 and lact. mucosae srv10 isolated from donkey milk. all major in vitro screening assays were employed to evaluate studied strains. both strains displayed good survivability at gastric ph 2.0, 0.3% bile and simulated oro-gastrointestinal fluid (above 88%). also, cultures demonstrated good cell surface hydrophobicity and auto-aggregation ability, clearly indi ... | 2020 | 31713771 |
relation between apgar scoring and physical parameters in 44 newborn amiata donkey foals at birth. | the assessment of the behavior and physiological parameters of neonatal foals is essential in the detection of early signs of illness. modified apgar scoring systems from human medicine exist and have been validated in foals as a guide for assessing neonatal viability after birth. this study evaluated the viability of 44 amiata donkey foals at birth, by assessing the apgar score and comparing the relationship between viability and various physical parameters. a total of 44 amiata donkey foals an ... | 2020 | 31711687 |
comparative metabolomics analysis of donkey colostrum and mature milk using ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry. | donkey milk has been widely shown to be an ideal substitute for human milk because of its similar composition. however, alterations to the composition of donkey milk during lactation have not been well studied. in this study, untargeted metabolomics with ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry were used to analyze and compare the metabolites in donkey colostrum (dc) and mature milk (dmm). two hundred seventy metabolites were characterized i ... | 2020 | 31677837 |
epidemiological study of equine piroplasmosis (theileria equi and babesia caballi) by microscopic examination and competitive-elisa in erbil province north-iraq. | equine piroplasmosis is a major tick-borne disease that can lead to serious health problems and economic losses in horse industry. the aim of the study is to determine the prevalence of theileria equi and babesia caballi in different species of equus namely (horse, mule, donkey and pony) by giemsa stained blood films and competitive elisa. | 2020 | 31673258 |
total and specific activities of superoxide dismutase (sod) in seminal plasma are related with the cryotolerance of jackass spermatozoa. | this study investigated whether the activities of four antioxidant enzymes present in jackass seminal plasma (sp), superoxide dismutase (sod), catalase (cat) and glutathione peroxidase (gpx) and glutathione reductase (gsr), are related to the sperm ability to withstand cryopreservation. eighteen ejaculates from 16 healthy jackasses were collected and split into two aliquots. the first one was centrifuged (3,000×g, 4 °c for 10 min) and used to determine the activities of these four enzymes in sp, ... | 2020 | 31778669 |
evaluation of the sedative and clinical effects of xylazine, detomidine, medetomidine and dexmedetomidine in miniature donkeys. | aim: to evaluate the sedative and clinical effects of i/v xylazine, detomidine, medetomidine and dexmedetomidine in miniature donkeys.methods: seven clinically healthy, male adult miniature donkeys with a mean age of 6 years and weight of 105 kg, were assigned to five i/v treatments in a randomised, cross-over design. they received either 1.1 mg/kg xylazine, 20 μg/kg detomidine, 10 μg/kg medetomidine, 5 μg/kg dexmedetomidine or saline, with a washout period of ≥7 days. the degree of sedation was ... | 2020 | 31778611 |
the cryoprotective effect of ficoll 70 on the post-warming survival and quality of cryotop-vitrified donkey embryos. | many domestic donkey breeds are at risk of extinction, there is a critical urgency for genome resource banking. in the present study, we examined whether the use of ficoll 70 added to the vitrification medium containing ethylene glycol (eg), dimethyl sulfoxide (dmso) and sucrose improves the cryotolerance of donkey in vivo derived embryos. day 7-8, grade 1-2 donkey embryos were measured and morphologically evaluated and then vitrified-warmed using the cryotop technique. before vitrification, emb ... | 2020 | 31757484 |
comparative expression of cell adhesion molecule1 (cadm1) in the testes of experimental mice and some farm animals. | cell adhesion molecule1 (cadm1) is a member of the immunoglobulin superfamily (igsf) that has been found in mammalian testis and plays a substantial role in cell-to-cell interaction via either hemophilic (between spermatogenic cells) or heterophilic (between spermatogenic and somatic sertoli cells) binding. the present study investigated the immunohistochemical localization of cadm1 in the testes of adult mice (mus musculus), as well as sexually mature bull (bos taurus), camel (camelus dromedari ... | 2020 | 31635798 |
genetic characteristics of theileria equi in zebras, wild and domestic donkeys in israel and the palestinian authority. | equine piroplasmosis (ep) is an important tick-borne disease of equids, caused by theileria equi and babesia caballi. it is endemic in most parts of the world, including israel, and has clinical and economic consequences. this study was set to evaluate the presence of ep parasites in domestic donkeys and in wild equids in israel and the palestinian authority (pa). to assess subclinical ep infection in 98 domestic donkeys (equus africanus asinus), 9 asiatic wild donkeys (equus hemionus), 8 zebras ... | 2020 | 31537490 |
dezhou donkey (equus asinus) milk a potential treatment strategy for type 2 diabetes. | donkey (equus asinus) milk has become a medical and nutrient product since ancient times. in addition, donkey milk was regarded as a medicinal food and substitute product for infant formula in some ancient western countries. chinese ancient medical books documented the medicinal value of donkey milk, using donkey milk to treat diabetes, cough and jaundice. | 2020 | 31494203 |
long-term treatment with naproxen changes the chemical coding of the porcine intramural duodenum neurons. | due to numerous therapeutic applications and high availability, non-steroidal anti-inflammatory drugs (nsaids) are the most widely used drugs worldwide. however, long-term use of these drugs can lead to damage to the gastrointestinal mucosa. the enteric nervous system (ens), which is part of the autonomic nervous system, controls most aspects of gastrointestinal activity. enteric neurons are characterized by considerable chemical plasticity and the appearance of a pathological factor results in ... | 2020 | 31610253 |
first report regarding the simultaneous molecular detection of anaplasma marginale and theileria annulata in equine blood samples collected from southern punjab in pakistan. | the present study was designed to check the molecular detection of anaplasma marginale and theileria annulata in blood samples of horses and donkeys collected from dera ghazi khan district in punjab and to document their phylogenetic origin and their association with studied epidemiological factors (sex and age) and complete blood count parameters, if any. | 2020 | 31721059 |
establishment and application of a 10-plex liquid bead array for the simultaneous rapid detection of animal species. | meat fraud and adulteration incidents occur frequently in almost all regions of the globe, especially with the increase in the world's population. to ensure the authenticity of meat products, we developed a 10-plex xmap assay to simultaneously detect ten animal materials: bovine, caprine, poultry, swine, donkey, deer, horse, dog, fox and mink. | 2020 | 31584699 |
factors associated with the prevalence of antibodies against theileria equi in equids of western pará, brazil. | the state of pará has one of the largest herds of equids (horse, donkey and mule) in brazil, most of these animals are found on cattle farms. equine theileriosis is a tick-borne disease caused by the parasite theileria equi and is characterized by fever, anaemia, icterus, intravascular haemolysis, haemoglobinuria, spleen and hepatomegaly, and even death. the present study aimed to determine the prevalence of antibodies against t. equi in equids in the western region of the state of pará, brazil, ... | 2020 | 31286674 |
anthelmintic activity of crude powder and crude aqueous extract of trachyspermum ammi on gastrointestinal nematodes in donkey (equus asinus): an in vivo study. | trachyspermum ammi has been used traditionally as a popular ethnobotanical plant in human and animal parasitic infestations. few scientific studies have been conducted on in vitro anthelmintic activity of t. ammi against various helminths and there is no study on its in vivo/in vitro anthelmintic properties against equine helminths. | 2020 | 31557509 |
prevalence of skin wounds in working donkeys in bukombe, tanzania. | preventable wounds are a common welfare issue in working donkeys in many countries. in the bukombe district of tanzania, there are estimated to be 3000 working donkeys, used primarily to transport loads for direct income generation. for historical reasons, oxen-yoke carts are used; their design is inappropriate for donkeys and results in serious neck wounds. the project aim was to assess the prevalence and nature of wounds in working donkeys. | 2020 | 31554710 |
fatal multiple outbreaks of equine influenza h3n8 in nigeria, 2019: the first introduction of florida clade 1 to west africa. | in december 2018, suspected outbreaks of equine influenza (ei) were observed in donkeys in sokoto state, in the extreme northwest of nigeria bordering the republic of the niger. equine influenza virus (eiv) subtype h3n8 was the etiologic agent identified in the outbreaks using real-time rt-qpcr and sequencing of both the partial haemagglutinin (ha) gene and the complete genome. since then the h3n8 virus spread to 7 of the 19 northern states of nigeria, where it affected both donkeys and horses. ... | 2020 | 32891950 |
fatal donkey bite in children: a case report. | although animal-inflicted injuries are a worldwide major public health problem, lethal donkey bites have very rarely been reported. we describe the case of a 21-month-old girl that was found dead in a farmyard next to a donkey that belonged to the family. examination of the body showed bite marks on the right side of the neck and the upper part of the shoulder. death was attributed to cervical trauma and spine injuries from a donkey attack. | 2020 | 32889631 |
therapeutic efficacy of phage piz sae-01e2 against abortion caused by salmonella enterica serovar abortusequi in mice. | salmonella enterica subsp. enterica serovar abortusequi is a frequently reported pathogen causing abortion in mares. in this study, the preventive and therapeutic effects of phage piz sae-01e2 against s abortusequi in a mouse model of abortion were investigated. phage piz sae-01e2 was stable at different temperatures (4 to 70°c) and ph values (ph 4 to 10) and could lyse the majority of the salmonella serogroup o:4 and o:9 strains tested (25/28). there was no lysogeny-related, toxin, or antibioti ... | 2020 | 32887718 |
an epidemiological survey of theileria equi parasite in donkeys (equus asinus) in egypt. | in the present study, we conducted an epidemiological survey of theileria equi, with sequencing analysis of the pcr product using blood-dna samples collected from donkeys (n = 149) reared in different egyptian provinces in lower egypt (menoufia and mersa matruh) and middle egypt (giza). all animals were tested for the presence of t. equi parasite using species-specific pcr assay targeting the equi merozoite antigen-1 (ema-1). nine- (6.04%) samples were positive for t. equi. the highest positive ... | 2020 | 32862920 |
analysis of theileria equi diversity in the gambia using a novel genotyping method. | theileria equi, one of the primary pathogens causing equine piroplasmosis, has previously been sub-classified into a number of clades on the basis of 18s ssu rrna gene sequence diversity. this partitioning of the parasite population has potential implications for host immunity, treatment and vaccine development. to detect and identify different clade genotypes among and within individual equine blood samples, a novel pcr-based technique was designed and optimized. theileria equi has only recentl ... | 2020 | 31845493 |
examination of jackass (equus asinus) accessory sex glands by b-mode ultrasound and of testicular artery blood flow by colour pulsed-wave doppler ultrasound: correlations with semen production. | the accessory sex glands play a major role in the production of seminal plasma, and testicular artery blood flow seems to strongly influence testicular function. however, very little ultrasound imaging of these organs has been undertaken in donkeys. the present work reports the results of such examinations in five jackasses along the year. the accessory glands were inspected by b-mode ultrasound while the testicular artery blood flow was assessed by colour pulsed-wave doppler ultrasound. the tes ... | 2020 | 31829461 |
design a highly specific sequence for electrochemical evaluation of meat adulteration in cooked sausages. | a specific and unique sequence probe was designed for detection of donkey adulteration in cooked sausages and its species specificity was confirmed bioinformatically in the common software and website (clustalx and ncbi). subsequently, a novel species-specific electrochemical dna probe (locked nucleic acid, lna) was synthesized and implemented in a construction of dna-based electrochemical genosensor for sensitive, convenient and selective detection of donkey adulteration. the electrochemical be ... | 2020 | 31818752 |
laparoscopic ovariectomy in standing mule mares. | mules are hybrids bred from the mating of a jack donkey and a horse mare, known for their strength and resistance and still used to work in agriculture. although they have been for long considered sterile, evidence of estrus cycle has been demonstrated together with abnormal behavior related to ovarian activity. in this study, a bilateral standing laparoscopic ovariectomy technique using the ligasure technology was applied in 10 mare mules for treating unwanted behavioral patterns. the technique ... | 2020 | 31864467 |
leptin receptor gene polymorphisms in some turkish donkey populations. | leptin receptor is a fundamental regulator in physiological functions of the regulation of food intake, energy homeostasis, immune function, and reproduction as well as on ovarian follicular cells on the placenta and lactating mammary glands. the aim of this study was to investigate the lepr gene polymorphism in 60 donkeys reared in thrace region of turkey. a 585 bp long partial intron 6, exon 7, intron 7, and exon 8 regions of lepr gene were amplified, and polymerase chain reaction products ana ... | 2020 | 31864461 |
quantitative lipidomics reveals alterations in donkey milk lipids according to lactation. | the composition of donkey milk is similar to that of human milk. however, the lipid content in donkey milk is lower than that in human milk. thus far, the lipid composition of donkey milk during lactation has not been well-studied. through mass spectroscopy-based quantitative lipidomics, we analyzed lipids in donkey colostrum (dc) and mature milk (dm). thirteen subclasses of 335 lipids were identified in both dc and dm; 60 lipids - 17 upregulated and 43 downregulated - were differentially regula ... | 2020 | 31784068 |
genetic characterization of piroplasms in donkeys and horses from nigeria. | equine piroplasmosis (ep) is a tick-borne disease of equids, caused by the two haemoprotozoal parasites: theileria equi and babesia caballi. nigeria constitutes a major crossroads of animal transport in west africa and may serve as a factor in ep dissemination in the region. the study aim was to characterize ep parasites in donkeys and horses in northern nigeria using a molecular approach. blood was collected from 57 donkeys and 47 horses. ep infection was detected and characterized by polymeras ... | 2020 | 32085574 |
mortality and operational attributes relative to feral horse and burro capture techniques based on publicly available data from 2010-2019. | management of excessive feral horse (equus ferus caballus) and burro (equus asinus) populations in the united states and globally has been a controversial subject for decades. i reviewed all available us federal feral horse and burro daily gather reports from 2010 to 2019 to extract equine species, technique (bait trapping or helicopter gathering), reason (emergency or other), number gathered, number of mortalities, and mortality attributes (acute or chronic/pre-existing condition, specific caus ... | 2020 | 32067672 |
evaluation of a marketed polyherbal dewormer against intestinal strongyles in naturally infected donkeys. | the study evaluated the effectiveness of a commercially available polyherbal dewormer to control intestinal strongyles in naturally infected donkeys. the animals were allotted to two groups: treated with the herbal dewormer (n=8) according to manufacturer recommendations and untreated control group (n=6). fecal samples were taken from each animal on days 0 (day of treatment), 14, 21 (day of second additional treatment), 35, and 42. faecal egg count reduction tests showed very negligible or no re ... | 2020 | 32063744 |
infestation rate of lice on donkeys in northeast tunisia. | the present survey was carried out during autumn 2013 (september, october and november) in five localities of jendouba district. a total number of 300 donkeys were examined and all present ectoparasites found on animals were collected and examined under stereomicroscope. the infestation rate by lice was estimated to 12.7% (38/300) there was no difference between infestation rates in males (11.5%; 22/192) and females (14.8%; 16/108). the highest infestation rate was observed in donkeys aged betwe ... | 2020 | 32057395 |
development and validation of a rapid kit for authenticity of murine meat in meat products with a species-specific pcr assay. | adulteration of meat products with murine meat poses a huge threat to consumer health and leads to serious disruption in food markets. species authentication of murine meat is still technically challenging. we, therefore, developed a species-specific pcr kit consisting of murine meat dna extraction, pcr reaction and identifying systems. we designed novel universal primers targeting highly conserved region on mitochondrial cytochrome b gene (cyt b) from four murines (lab rats, lab mice, wild rat ... | 2020 | 32039648 |
doppler evaluation of renal resistivity index in healthy conscious horses and donkeys. | the renal resistive index (rri) is used as a measurement of downstream resistance in arteries. the aim of this study was to assess the rri of the arcuate arteries by pulsed-wave doppler ultrasonography in healthy conscious horses and donkeys, and to verify any differences related to age, breed, bodyweight (bw) or body condition (bcs). thirty-three healthy conscious horses and nine donkeys had their systolic and diastolic flow velocities at the level of the arcuate arteries estimated by pulsed-wa ... | 2020 | 32027703 |
the recycling of the expired donkey-hide gelatin pulp for n/s co-doped hollow carbon nano-spheres anode in sodium ion battery. | how to reasonably manage and reutilize the waste expired liquid medicines has always been a puzzling public concern. for this reason, the waste expired medicine of donkey-hide gelatin pulp was recycled by hydrothermal carbonization and hard template for n/s co-doped hard carbon material, and its electrochemical na-storage performances were also evaluated. the results showed that the resultant n/s co-doped hard carbon material manifested the morphology of hollow nano-spheres with the mean diamete ... | 2020 | 32026369 |
zoonotic potential of enterocytozoon bieneusi and giardia duodenalis in horses and donkeys in northern china. | limited data are available on infection rates and genetic identity of enterocytozoon bieneusi and giardia duodenalis in horses and donkeys. in this study, 865 fecal specimens were collected from donkeys (n = 540) and horses (n = 325) in three provinces and autonomous regions in northern china during 2015-2019. enterocytozoon bieneusi was detected and genotyped by pcr and sequence analyses of the ribosomal internal transcribed spacer (its) and g. duodenalis was detected and genotyped by pcr and s ... | 2020 | 32006227 |
superstition, misconceptions, and magical beliefs in burns patients-a cross-sectional study of 100 patients. | superstitious beliefs have been frequently encountered in our day-to-day practices among patients and caretakers. though this is a common phenomenon, there is a paucity of data pertaining to these beliefs due to various reasons. many of these beliefs are deep engraved into the culture and mindsets of the population. this is an observational study performed in tertiary burn care center in india during period october 1, 2018 to january 31, 2019. data from 100 patient units were collected through a ... | 2020 | 31996923 |
development of a real-time pcr assay for the detection of donkey (equus asinus) meat in meat mixtures treated under different processing conditions. | in this study, a donkey-specific primer pair and probe were designed from mitochondrial cytochrome b gene for the detection of raw donkey meat and different processed meat mixtures. the pcr product size for donkey dna was 99 bp, and primer specificity was verified using 20 animal species. the limit of detection (lod) was examined by serially diluting donkey dna. using real-time pcr, 0.001 ng of donkey dna could be detected. in addition, binary meat mixtures with various percentages of donkey mea ... | 2020 | 31991914 |
serological and molecular detection of selected hemoprotozoan parasites in donkeys in west omdurman, khartoum state, sudan. | in sudan, donkeys are important animals, providing transportation and income possibilities. however, the prevalence of parasites in donkeys in sudan has not been thoroughly characterized. accordingly, in this study, we aimed to detect selected hemoprotozoan parasites in donkeys in west omdurman, khartoum state, sudan, wherein people depend mainly on donkeys for their daily life. in total, 198 blood samples collected from donkeys in a local market in west omdurman, were screened using serological ... | 2020 | 31969541 |
seminal plasma has limited counteracting effects following induction of oxidative stress in donkey spermatozoa. | the aim of this study was to evaluate the response of donkey spermatozoa to oxidative stress induced by hydrogen peroxide, and to determine whether the presence of seminal plasma modulates the sperm response to that stress. nine ejaculates were collected, extended in skim milk extender and split into two aliquots. seminal plasma was removed from the first but not second aliquot. samples were subsequently split into four aliquots supplemented with different concentrations of commercial hydrogen p ... | 2020 | 31962061 |
comparative effect of epidural administration of xylazine or dexmedetomidine on echocardiographic dimensions and cardiac indices in clinically healthy donkeys (equus asinus). | the aim of the present study was to assess and compare the changes of the echocardiographic dimensions and cardiac function indices after epidural injection of xylazine or dexmedetomidine in clinically healthy donkeys. in an experimental prospective randomized cross-over study, 10 healthy adult donkeys were injected with saline solution, xylazine (0.20 mg kg-1), and dexmedetomidine (0.005 mg kg-1) into the epidural space between the second and third coccygeal vertebrae. echocardiographic dimensi ... | 2020 | 31952632 |
prevalence of trypanosoma evansi in livestock in palestine. | trypanosoma evansi is the causative agent of surra, a disease that occurs in many animal species. the disease is responsible for substantial losses in global production and can be fatal if not diagnosed early. this study aims to determine the prevalence of t. evansi in livestock, equids and dromedary camels in palestine. | 2020 | 31931864 |
molecular and genomic characterization of a novel equine molluscum contagiosum-like virus. | cases of pox-like lesions in horses and donkeys have been associated with poxviruses belonging to different genera of the family poxviridae. these include the orthopoxviruses vaccinia virus (vacv), horsepoxvirus (hpxv) and cowpoxvirus (cpxv), as well as a potentially novel parapoxvirus and molluscum contagiosum virus (mocv). however, with the exception of vacv, hpxv and cpxv, the genomic characterization of the causative agents remains largely elusive with only single short genome fragments avai ... | 2020 | 31922947 |
the continental atlas of tsetse and african animal trypanosomosis in nigeria. | tsetse-transmitted trypanosomosis remains a major animal health problem in nigeria, in a context where changes in land cover, climate and control interventions are modifying its epidemiological patterns. evidence-based decision making for the progressive control of the disease requires spatially-explicit information on its occurrence and prevalence, as well as on the distribution and abundance of the tsetse vector. in the framework of the continental atlas of tsetse and african animal trypanosom ... | 2020 | 31904345 |
diagnostic orientation values for acth and other parameters for clinically healthy donkeys and mules (insulin, triglycerides, glucose, fructosamines, and ɣ-gt). | pituitary pars intermedia dysfunction is the most prevalent endocrine disease in horses. although donkeys and mules may also be affected, only a few data have been published. reference values for diagnostic parameters, such as adrenocorticotropic hormone (acth), are especially scarce or even lacking. therefore, in the present study, available data from the literature have been verified and completed to facilitate a reliable diagnosis. clinical inspections and haematological and biochemical exami ... | 2020 | 31900994 |
structural analysis of glycosaminoglycans from colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry. | colla corii asini (cca) made from donkey-hide has been widely used as a traditional animal-based chinese medicine. chondroitin sulfate (cs), dermatan sulfate (ds) and hyaluronic acid (ha) are structurally complex classes of glycosaminoglycans (gags) that have been implicated in a wide range of biological activities. however, their possible structural characteristics in cca are not clear. in this study, gag fractions containing cs/ds and ha were isolated from cca and their disaccharide compositio ... | 2020 | 31900724 |
comparative analysis of whey proteins in donkey colostrum and mature milk using quantitative proteomics. | donkey milk is attracting increasing attention as a nutritional milk source similar to human milk. in this study, we carried out qualitative and quantitative analysis of the donkey whey proteome using a label-free proteomic approach, combined with parallel reaction monitoring (prm) as a validation method. a total of 300 whey proteins were identified in donkey colostrum (dc) and donkey mature (dm) milk, of which 18 were differentially expressed (p < 0.05) between the two types of milk. gene ontol ... | 2020 | 31882075 |
detection of hepatitis e virus genotypes 3 and 4 in donkeys in northern china. | hepatitis e virus (hev) is the causative agent of acute self-limiting hepatitis in humans in developing countries. hepatitis e virus rna was first detected in donkeys in spain, but little is known about the possible presence of hev in donkeys in china. | 2020 | 31746470 |
potential dual expansion of domesticated donkeys revealed by worldwide analysis on mitochondrial sequences. | molecular studies on donkey mitochondrial sequences have clearly defined two distinct maternal lineages involved in domestication. however, domestication histories of these two lineages remain enigmatic. we therefore compared several population characteristics between these two lineages based on global sampling, which included 171 sequences obtained in this study (including middle asian, east asian, and african samples) plus 536 published sequences (including european, asian, and african samples ... | 2020 | 31709786 |
serological evidence of leptospirosis in iran; a systematic review and meta-analysis. | leptospirosis is a neglected infectious zoonotic disease that affects domestic animals and wildlife as well as humans. although leptospirosis is known as an endemic disease in iran, there is no accurate information on the overall prevalence of this disease in humans and animals. the aim of this systematic review and meta-analysis was to estimate the seroprevalence of leptospirosis among human and domestic and wild animals in iran. a systematic review of english and persian articles (since 1998 t ... | 2020 | 31698052 |
bearing the brunt: mongolian khulan (equus hemionus hemionus) are exposed to multiple influenza a strains. | the majority of influenza a virus strains are hosted in nature by avian species in the orders of anseriformes and charadriformes. a minority of strains have been able to cross species boundaries and establish themselves in novel non-avian hosts. influenza viruses of horses, donkeys, and mules represent such successful events of avian to mammal influenza virus adaptation. mongolia has over 3 million domestic horses and is home to two wild equids, the asiatic wild ass or khulan (equus hemionus hem ... | 2020 | 32122608 |
utility of a fluid library with samples of humans, reservoirs and vectors collected in filter paper, for retrospective diagnosis of american trypanosomiasis in endemic areas of venezuela. | we define a fluid library as a library of samples of different biological fluids (from humans, animals or vectors) collected and properly stored on filter paper, which allows retrospective studies, especially of diagnosis or detection of infectious agents in these samples, using different techniques. the objective of this work was the retrospective diagnosis of american trypanosomiasis by pcr in a venezuelan endemic area using a fluid library. | 2020 | 32930930 |
cloning and expression analysis of the follicle-stimulating hormone receptor (fshr) gene in the reproductive axis of female yaks (bos grunniens). | follicle-stimulating hormone receptor (fshr) plays a central role in promoting follicle maturation through the follicle-stimulating hormone (fsh)-mediated camp pathway in animals. the objectives of the present study were to clone the fshr gene of yaks (bos grunniens) and compare differences in fshr mrna expression in the reproductive axis between yaks and cattle. hypothalamus, anterior pituitary, oviduct, ovary, and uterus tissue samples were collected from adult female yaks (n = 5) and cattle ( ... | 2020 | 31479928 |
tyrosine hydroxylase containing neurons in the thalamic reticular nucleus of male equids. | here we report the unusual presence of thalamic reticular neurons immunoreactive for tyrosine hydroxylase in equids. the diencephalons of one adult male of four equid species, domestic donkey (equus africanus asinus), domestic horse (equus caballus), cape mountain zebra (equus zebra zebra) and plains zebra (equus quagga), were sectioned in a coronal plane with series of sections stained for nissl substance, myelin, or immunostained for tyrosine hydroxylase, and the calcium-binding proteins parva ... | 2020 | 33086098 |
the non-invasive measurement of faecal immunoglobulin in african equids. | eco-immunological research is encumbered by a lack of basic research in a wild context and by the availability of few non-invasive tools to measure the internal state of wild animals. the recent development of an enzyme-linked immunosorbent assay for measuring immunoglobulins in faecal samples from soay sheep prompted us to optimize such an assay to measure immunoglobulin a (iga: an antibody associated with parasitic nematode fecundity) in faecal samples from equids. we measured total iga in dom ... | 2020 | 32528845 |
anthelmintic resistance in strongylids (nematoda: strongylidae) parasitizing wild and domestic equids in the askania nova biosphere reserve, ukraine | resistance of strongylids in domestic horses to benzimidazole anthelmintics (bz) has been detected worldwide; however, information on the presence of bz-resistance in wild equids has not been published to date. the purpose of this study was to analyze the manifestations of the bz resistance in strongylids in domestic and wild equids kept in the askania nova biosphere reserve, ukraine. four species of equids: domestic horses and shetland ponies (equus caballus), donkeys (e. asinus), plains zebras ... | 2020 | 32198995 |
effect of social organisation on interspecific differences in overmarking behaviour of foals in african equids. | overmarking remains an unstudied topic in juvenile mammals. we have previously documented a very high rate of overmarking by foals in four captive african equid species: mountain zebra (equus zebra), plains zebra (equus quagga), grévy's zebra (equus grevyi), and african wild ass (equus africanus). african equids vary interspecifically in their social organisation. since differences in social organisation affect many mammalian behaviours, in this study we investigated interspecific differences in ... | 2020 | 31728806 |
kinematic, bioenergetic and oxidative evaluations of donkey sperm preserved at +4°c. | information on donkey sperm bioenergetics, kinetics and oxidative status is scarce even though crucial for development of reproductive technologies and germplasm conservation. for these reasons, it is interesting to monitor sperm kinetics, bioenergetics, and oxidative status during sperm storage at +4°c and with several sperm extenders and concentrations. donkey semen was collected from three jackasses, three times each. it was diluted with four extenders (kenney, equiplus, inra96 or hippex), se ... | 2020 | 32285764 |
evaluation of xylazine-alfaxalone anesthesia for field castration in donkey foals. | to evaluate the anesthetic and cardiopulmonary effects of xylazine-alfaxalone anesthesia in donkey foals undergoing field castration. | 2020 | 32278647 |
sequence characterization and n-glycoproteomics of secretory immunoglobulin a from donkey milk. | secretory immunoglobulin a (siga) is the major antibody present in the human milk where it confers passive immunity to neonates. other than human, non-ruminants such as equine, swine etc., also possess siga in milk but detailed characterization is limited. in the present study, we characterized siga from donkey milk for amino acid sequence and n-glycosylation through lc-ms/ms analysis. the complete amino acid sequence of alpha chain constant region (ch) was elucidated. the sequence analysis of v ... | 2020 | 32251747 |
effects of red-light irradiation on the function and survival of fresh and liquid-stored donkey semen. | this study sought to determine whether sperm irradiation using a light emission diode (led) at 620-630 nm affects the motility, membrane integrity (viability), mitochondrial activity and intracellular levels of reactive oxygen species (ros) in fresh diluted and liquid-stored donkey semen. with this purpose, sixteen ejaculates (eight fresh diluted and eight cooled-stored) were collected from eight adult jackasses. fresh semen samples were diluted in kenney extender and stimulated with red-light a ... | 2020 | 32247951 |
genotypes and haplotype combination of acsl3 gene sequence variants is associated with growth traits in dezhou donkey. | exploring molecular markers related to economic traits of livestock is of great significance to breeding. long-chain fatty acid coa synthetase (acsl) plays a crucial role in lipid synthesis and metabolism, which may affect animal growth. this study was to investigate the polymorphism of acsl gene and its association with the growth trait of the donkey. three insertions and two deletions were detected on the introns of acsl3 gene in 450 dezhou donkeys using polyacrylamide gel electrophoresis. aft ... | 2020 | 32217139 |
purification of animal immunoglobulin g (igg) using peptoid affinity ligands. | the availability of highly pure animal antibodies is critical in the production of diagnostic tools and biosensors. the peptoid pl16, previously isolated from an ensemble of peptoid variants of the igg-binding peptide hwrgwv, was utilized in this work as affinity ligand on workbeads resin for the purification of immunoglobulin g (igg) from a variety of mammalian sources and chicken immunoglobulin y (igy). the chromatographic protocol initially optimized for murine serum and ascites was subsequen ... | 2020 | 32216050 |
molecular detection and identification of leishmania dna and blood meal analysis in phlebotomus (larroussius) species. | phlebotomus (larroussius) perniciosus and canis familiaris are respectively the only confirmed vector and reservoir for the transmission of leishmania (l.) infantum mon-1 in tunisia. however, the vector and reservoir hosts of the two other zymodemes, mon-24 and mon-80, are still unknown. the aim of this study was to analyze the l. infantum life cycle in a tunisian leishmaniasis focus. for this purpose, we have focused on: i) the detection, quantification and identification of leishmania among th ... | 2020 | 32214313 |
seroprevalence of brucella antibodies in donkeys (equus asinus) in yobe south senatorial zone, northeastern nigeria. | a cross-sectional survey was conducted to determine the seroprevalence and risk factors influencing the presence of brucella spp. antibodies in donkeys in yobe south senatorial zone, nigeria. the study was aimed at determining the importance of brucella spp. infection in donkeys (equus asinus). a total of 200 sera samples from of 105 males and 95 female donkeys were collected and screened for brucellosis using the rose bengal plate test (rbpt) and the indirect enzyme-linked immunosorbent assay ( ... | 2020 | 32206033 |
evaluation of acquired passive immunity in mule foals up to 60 days of age. | ten mule foals were used to investigate serum igg concentration. blood was collected from mares and foals on the day following parturition (d1), at 48 and 72 hr after birth (d2 and d3), and on d7, d14, d21, d28, d35, d42, and d60. serum igg concentration was determined by zinc sulfate turbidity test and was above 400 mg/dl at all time points, except for one foal on d2. mean values were above 800 mg/dl from d1 to d7 and at d60. the difference between mares and foals at each collection time was si ... | 2020 | 32206032 |
variation in disease phenotype is marked in equine trypanosomiasis. | equine trypanosomiasis is a severe and prevalent disease that has the greatest impact globally upon working equids due to its distribution across lower income countries. morbidity and mortality rates are high; disease management strategies in endemic regions are ineffective and cost prohibitive. individual variation in disease phenotype in other species suggests host factors could reveal novel treatment and control targets but has not been investigated in equids. | 2020 | 32199454 |
donkey milk inhibits triple-negative breast tumor progression and is associated with increased cleaved-caspase-3 expression. | donkey milk is considered an ideal substitute for human milk and is considered a potential complementary dairy product for the treatment of a variety of human diseases, including cancer. the purpose of this study was to investigate the inhibitory effect of donkey colostrum (dc) and mature milk (dm) on 4t1 triple-negative breast cancer (tnbc) tumors in mice. metabolomics analyses showed that a total of 476 possible metabolites were found in both types of milk. among them, 34 differential metaboli ... | 2020 | 32191229 |
cross-anatomical, radiographic and computed tomographic study of the stifle joint of donkeys (equus africanus asinus). | the present investigation was conducted to provide a full anatomical description of the stifle joint of donkeys using 3d computed tomography imaging technique, in addition to the classic anatomical methods, such as radiography and cross-anatomical sectioning. the radiography and ct imaging of stifle joint were interpreted in comparison with cross-sectional anatomical sections. volume-rendering reconstruction techniques (3d-ct) were used to describe the anatomical structure of stifle joint. the u ... | 2020 | 32175631 |
banat donkey, a neglected donkey breed from the central balkans (serbia). | the dominant donkey breed in the balkans is the mid-sized balkan donkey with a grey to chocolate coat color. local breeders from serbia, however, still maintain a few larger individuals of a lighter coat color, named banat donkey, and speculate that they are descendants of a spanish donkey heard that had been transferred to the banat region by the hapsburg queen maria theresa in the xviii century for a specific purpose, to work in local vineyards. we have previously found a unique nuclear gene-p ... | 2020 | 32175186 |
a cross-sectional survey of foaling-related parameters of jennies (equus asinus) under smallholder farm conditions in northeast china. | it is important to assess the reproductive efficiency and improve the reproductive management to promote the donkey population development. the overall foaling-related parameters of jennies under smallholder farm conditions in china were hardly under investigation. a cross-sectional survey of randomly selected 694 smallholder farms was conducted in 40 villages in the north, east, and south areas of western liaoning province and eastern inner mongolia autonomous region between march and july, 201 ... | 2020 | 32172918 |
performance of host-associated genetic markers for microbial source tracking in china. | numerous genetic markers have been developed to establish microbial source tracking (mst) assays in the last decade. however, the selection of suitable markers is challenging due to a lack of understanding of fundamental factors such as sensitivity, specificity, and concentration in target/nontarget hosts, especially in east asia. in this study, a total of 506 faecal samples comprised of human and 12 nonhuman hosts were collected from 28 cities across china and tested for marker performance char ... | 2020 | 32171096 |
first highly sensitive and specific competitive elisa for detection of bovine besnoitiosis with potential as a multi-species test. | serological cross-reactions represent a serious problem in some currently available tests to diagnose besnoitia infections in many species including cattle, caribou and donkeys. false-positive results are due to the low positive-predictive value of these serological tests for besnoitiosis. these tests therefore have clear limitations if large herds are screened in areas with low prevalence, since increased numbers of false-positive reactions require confirmatory testing by alternative serologica ... | 2020 | 32169472 |
biochemical characterisation and application of keratinase from bacillus thuringiensis mt1 to enable valorisation of hair wastes through biosynthesis of vitamin b-complex. | this study reports on the exploitation of keratinous hydrolysate by keratinase enzymes to produce vitamin b-complex. toward this end, keratinase enzyme was produced by bacillus thuringiensis strain mt1, newly isolated from cattle-yard utilising donkey hairs. scanning electron microscope (sem) and fourier transform infrared spectrophotometer (ftir) analyses demonstrated hairs disintegration and the disruption of the disulphide bonds of the keratin structure, respectively. the biochemical characte ... | 2020 | 32151720 |
the prevalence of uveitis in a population of donkeys in the uk. | uveitis is reported to be a leading cause of blindness in horses. little work has been undertaken to date to investigate whether donkeys are affected by a similar disease prevalence. | 2020 | 32145036 |
col2a1 and caspase-3 as promising biomarkers for osteoarthritis prognosis in an equus asinus model. | osteoarthritis (oa) is one of the most degenerative joint diseases in both human and veterinary medicine. the objective of the present study was the early diagnosis of oa in donkeys using a reliable grading of the disease based on clinical, chemical, and molecular alterations. oa was induced by intra-articular injection of 25 mg monoiodoacetate (mia) as a single dose into the left radiocarpal joint of nine donkeys. animals were clinically evaluated through the assessment of lameness score, radio ... | 2020 | 32111016 |
childhood horse and donkey bites; a single tertiary health center experience in a rural area. | the aim of this study was to examine the characteristics of patients presenting to a pediatric emergency department in a rural province of turkey due to horse and donkey bites and to analyze whether these features differ from those of more common animal bites in rural areas. | 2020 | 32110542 |
monitoring acute pain in donkeys with the equine utrecht university scale for donkeys composite pain assessment (equus-donkey-compass) and the equine utrecht university scale for donkey facial assessment of pain (equus-donkey-fap). | objective pain assessment in donkeys is of vital importance for improving welfare in a species that is considered stoic. this study presents the construction and testing of two pain scales, the equine utrecht university scale for donkey composite pain assessment (equus-donkey-compass) and the equine utrecht university scale for donkey facial assessment of pain (equus-donkey-fap), in donkeys with acute pain. a cohort follow-up study using 264 adult donkeys (n = 12 acute colic, n = 25 acute orthop ... | 2020 | 32098391 |
equine milk production and valorization of marginal areas-a review. | the equine dairy chain is renewing the interest toward horse and donkey breeding for the production of milk with potential health promoting properties. the dairy equine chain for human consumption could contribute to the rural eco-sustainable development for the micro-economies of those areas threatened by marginalization. as a part of the whole equine industry, and its possible impact in the modern and future society, the main traits of the equine dairy enterprise are reviewed with a special fo ... | 2020 | 32098374 |
genetic characterization of hydatid cysts of different intermediate hosts. | cystic echinococcosis is an important cosmopolitan parasitic zoonosis that causes public health and economic problems in egypt. the present study was undertaken to identify genotypes of hydatid cyst (hc) dna isolated from different animal isolates and to identify the genotype of secondary hydatid cysts (hcs) developed in rabbits experimentally infected with camel hc for detection of any genetic mutation. in the present study, we extracted dna from the germinal layers of 8 hcs collected from 3 ca ... | 2020 | 32855606 |
surgical castration versus chemical castration in donkeys: response of stress, lipid profile and redox potential biomarkers. | castration is a husbandry practice raising important questions on the welfare and physiological status of farm animals. searching for effective castration methods that minimally compromise the body physiology is worthy of attention. therefore, this study aimed to evaluate the differential response of biological systems in donkeys to surgical castration versus the chemical one by cacl2 with special emphasis on stress, lipid profile, and oxidative stress biomarkers. donkeys were divided randomly a ... | 2020 | 32847551 |
peste des petits ruminants virus transmission scaling and husbandry practices that contribute to increased transmission risk: an investigation among sheep, goats, and cattle in northern tanzania. | peste des petits ruminants virus (pprv) causes an infectious disease of high morbidity and mortality among sheep and goats which impacts millions of livestock keepers globally. pprv transmission risk varies by production system, but a deeper understanding of how transmission scales in these systems and which husbandry practices impact risk is needed. to investigate transmission scaling and husbandry practice-associated risk, this study combined 395 household questionnaires with over 7115 cross-s ... | 2020 | 32847058 |
vitrification of donkey sperm: is it better using permeable cryoprotectants? | vitrification by direct exposure of sperm to liquid nitrogen is increasing in popularity as an alternative to conventional freezing. in this study, the effect of permeable cryoprotectant agents for donkey sperm vitrification was compared to an extender containing non-permeable cryoprotectants. first, three different concentrations of sucrose (0.1, 0.2, and 0.3 molar, m) and bovine serum albumin, bsa (1, 5, and 10%) were compared. secondly, the concentration of non-permeable agents producing the ... | 2020 | 32825370 |
development of a donkey grimace scale to recognize pain in donkeys (equus asinus) post castration. | the objectives of this study were to establish a donkey ethogram, followed by a donkey grimace scale to be applied to donkeys pre- and post-castration and to test if there was a notable difference in scores based on observer knowledge, gender, and experience, which could reveal possible discomfort/pain. nine healthy male adult donkeys were surgically castrated. fifty-four photos were selected from frontal, lateral, and body views taken pre- and post-castration. observers ranging from minimal to ... | 2020 | 32823676 |
characterizing the serum proteome of donkeys (equus asinus). | serum and plasma are commonly used in clinical practice considering the widely accepted fact that the "normal" protein expression pattern of a healthy animal changes under disease conditions. we herein used a label-free mass spectrometry-based quantitative proteomics approach to characterize the serum proteome of donkeys. a total of 277 unique proteins were identified from 2,388 unique peptides. gene ontology analyses showed that the most frequent processes were related to metabolic activities a ... | 2020 | 32797796 |
evaluation of brix refractometry for the estimation of colostrum quality in jennies. | donkey placenta does not allow the passage of immunoglobulins; thus, foals are born hypogammaglobulinemic and an adequate intake of high-quality colostrum in the first 24 hours of life is crucial for the surviving. the study aims to assess the relation between colostrum immunoglobulin g (igg) concentration evaluated by the single radial immunodiffusion (srid) test and the brix refractometer in donkeys to establish a cutoff value for high quality of colostrum based on brix refractometry. colostru ... | 2020 | 32797794 |
molecular analysis and risk factors associated with theileria equi infection in domestic donkeys and mules of punjab, pakistan. | theileriosis is an important disease of economic significance which badly affects the equine husbandry of developing countries. the present study was planned to investigate the molecular prevalence of theileriosis, associated risk factors, and alterations in hematological parameters of donkeys and mules from district mianwali, punjab, pakistan. blood samples from 150 equids (n = 75 donkeys; n = 75 mules) were examined microscopically, and the genomic dna from each sample was processed for the am ... | 2020 | 32797787 |
reference values and repeatability of transabdominal ultrasonographic gastrointestinal tract thickness and motility in healthy donkeys (equus asinus). | the present study aimed to provide reference ranges for the wall thickness and motility pattern of the gastrointestinal tract from a sample of donkeys (equus asinus) population using b-mode ultrasonography. in the present study, 30 clinically healthy donkeys (equus asinus) (15 males and 15 females), aged 2-20 year old and weighed 100-280 kg were randomly selected for b-mode ultrasonographic scanning of the abdomen. the wall thickness of the stomach, duodenum, jejunum, left colon, right colon, an ... | 2020 | 32797781 |
effect of the depth of insertion of the thermometer on the rectal temperature of donkeys during the hot-dry season in a tropical savannah. | the objective of the present study was to evaluate the effect of the depth of insertion and environmental parameters on the rectal temperature (rt) in donkeys during the hot-dry season in a tropical savannah zone of nigeria. the experimental subjects were comprised of thirty donkeys divided into three groups based on age: group i, 10 foals (40.67 ± 2.20 kg; 1.50 ± 0.02 months); group ii, 10 yearlings (91.53 ± 0.54 kg; 1.51 ± 0.01 years); and group iii, 10 adults (140 ± 0.71 kg; 8.03 ± 0.06 years ... | 2020 | 32797777 |
comparative efficacy of histrelin acetate and hcg for inducing ovulation in brazilian northeastern jennies (equus africanus asinus). | the goal of this study was to compare the efficiency of histrelin acetate (gnrh analog) and human chorionic gonadotropin (hcg) to hasten ovulation in brazilian northeastern jennies (equus africanus asinus). thirty cycles of ten jennies were randomly assigned in one of the three groups: g0 (control group), saline; g1, 250 μg of histrelin acetate; g2, 2500 iu of hcg. jennies were evaluated by transrectal palpation and ultrasonography, and had the administration of an ovulation-inducing agent when ... | 2020 | 32797776 |