Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| identification of optimal feedback control rules from micro-quadrotor and insect flight trajectories. | this paper presents "optimal identification," a framework for using experimental data to identify the optimality conditions associated with the feedback control law implemented in the measurements. the technique compares closed loop trajectory measurements against a reduced order model of the open loop dynamics, and uses linear matrix inequalities to solve an inverse optimal control problem as a convex optimization that estimates the controller optimality conditions. in this study, the optimal i ... | 2018 | 29299686 |
| experimental evolution of infectious behaviour in a facultative ectoparasite. | parasitic lifestyles have evolved many times in animals, but how such life-history strategies evolved from free-living ancestors remains a great puzzle. transitional symbiotic strategies, such as facultative parasitism, are hypothesized evolutionary stepping stones towards obligate parasitism. however, to consider this hypothesis, heritable genetic variation in infectious behaviour of transitional symbiotic strategies must exist. in this study, we experimentally evolved infectivity and estimated ... | 2017 | 29282821 |
| colour polymorphic lures exploit innate preferences for spectral versus luminance cues in dipteran prey. | theory predicts that colour polymorphism may be favored by variation in the visual context under which signals are perceived. the context encompasses all environmental determinants of light availability and propagation, but also the dynamics of perception in receivers. color vision involves the neural separation of information into spectral versus luminance channels, which often differentially guide specific tasks. here we explicitly tested whether this discrete perceptual basis contributes to t ... | 2017 | 28806928 |
| mitey costly: energetic costs of parasite avoidance and infection. | parasites reduce host fitness via perturbations to host energy allocation, growth, survival, and reproduction. here, we investigate the independent effects of parasite exposure and infection on host metabolic rate. our study focuses on drosophila hydei and a naturally occurring ectoparasitic mite, macrocheles muscaedomesticae. we use flow-through respirometry to measure the metabolic rate of flies during the period of exposure (preinfection) and during mite attachment. flies were exposed to mite ... | 2017 | 28414262 |
| separating multiple sources of variation on heat resistance in drosophila hydei. | while numerous insect studies have demonstrated the effects environmental conditions, genetic variation and other factors have on thermal resistance, often showing patterns consistent with adaptive plasticity and local adaptation, few experiments have considered the effects of multiple factors simultaneously. here however, we have investigated the impact of sex, rearing conditions, hardening, population, and laboratory rearing period on adult heat resistance in stocks of drosophila hydei, a cosm ... | 2017 | 27816712 |
| nondisjunction of the x chromosomes in females of drosophila hydei. | 2017 | 5883027 | |
| the effect of changes in the respiratory metabolism upon genome activity. a correlation between induced gene activity and an increase in activity of a respiratory enzyme. | the in vitro regression of experimentally induced chromosome puffs was investigated in explanted salivary gland chromosomes of drosophila hydei. it was observed that the regression of the puffs 2-32a, 2-36a, 2-48c, and 4-81b is accelerated if substrates for the respiratory metabolism are supplied to the cells. a similar effect can be produced by addition of kcn or oligomycin to medium in which intact salivary glands are incubated. the acceleration of puff regression by these substances occurs no ... | 2016 | 5076777 |
| mite choice generates sex- and size-biased infection in drosophila hydei. | heterogeneities in parasite infection among conspecific hosts often manifest as sex- or size-biased infections, which are typically attributed to differential host susceptibility and exposure. since parasite fitness is often tied to host quality, host preference by parasites is likely to be under strong selection. we test the hypothesis that host preference is sufficient to generate variability in infection rate among conspecifics. specifically, we ask whether the mite macrocheles muscaedomestic ... | 2016 | 26997035 |
| pollination of specklinia by nectar-feeding drosophila: the first reported case of a deceptive syndrome employing aggregation pheromones in orchidaceae. | the first documented observation of pollination in pleurothallidinae was that of endrés, who noticed that the 'viscid sepals' of specklinia endotrachys were visited by a 'small fly'. chase would later identify the visiting flies as being members of the genus drosophila. this study documents and describes how species of the s. endotrachys complex are pollinated by different drosophila species. | 2015 | 26071932 |
| rapid spread of the defensive endosymbiont spiroplasma in drosophila hydei under high parasitoid wasp pressure. | maternally transmitted endosymbionts of insects are ubiquitous in nature and play diverse roles in the ecology and evolution of their hosts. to persist in host lineages, many symbionts manipulate host reproduction to their advantage (e.g. cytoplasmic incompatibility and male-killing), or confer fitness benefits to their hosts (e.g. metabolic provisioning and defense against natural enemies). recent studies suggest that strains of the bacterial genus spiroplasma protect their host (flies in the g ... | 2015 | 25764546 |
| body saccades of drosophila consist of stereotyped banked turns. | the flight pattern of many fly species consists of straight flight segments interspersed with rapid turns called body saccades, a strategy that is thought to minimize motion blur. we analyzed the body saccades of fruit flies (drosophila hydei), using high-speed 3d videography to track body and wing kinematics and a dynamically scaled robot to study the production of aerodynamic forces and moments. although the size, degree and speed of the saccades vary, the dynamics of the maneuver are remarkab ... | 2015 | 25657212 |
| dna damage in haemocytes and midgut gland cells of steatoda grossa (theridiidae) spiders exposed to food contaminated with cadmium. | the aim of this study was to assess the genotoxic effects of cd on haemocytes and midgut gland cells of web-building spiders, steatoda grossa (theridiidae), exposed to the metal under laboratory conditions. analyzes were conducted on adult females and males, fed for four weeks with cadmium-contaminated drosophila hydei flies, grown on a medium suplemented with 0.25 mm cdcl2. the comet assay, providing a quantitative measure of dna strand breaks, was used to evaluate the dna damage caused by the ... | 2015 | 25531832 |
| [differential radiosensitivity of the germ cells in drosophila hydei males, lethal rates and the relevance of the species-specific genetic organization (author's transl)]. | 2015 | 4469156 | |
| [regulation of ion concentration by drosophila hydei na+, k+, ca2+, and cl-]. | 2015 | 4425097 | |
| minos as a novel tc1/mariner-type transposable element for functional genomic analysis in aspergillus nidulans. | transposons constitute powerful genetic tools for gene inactivation, exon or promoter trapping and genome analyses. the minos element from drosophila hydei, a tc1/mariner-like transposon, has proved as a very efficient tool for heterologous transposition in several metazoa. in filamentous fungi, only a handful of fungal-specific transposable elements have been exploited as genetic tools, with the impala tc1/mariner element from fusarium oxysporum being the most successful. here, we developed a t ... | 2015 | 26021704 |
| differentiation of transplanted larval salivary glands of drosophila hydei in adults of the same species. | 2014 | 4222967 | |
| sex-specific divergence for body size and desiccation-related traits in drosophila hydei from the western himalayas. | sex-specific-differences are a widespread source of genetic variation in various drosophila species. in the present study, we have examined desiccation survival in males and females of drosophila hydei from colder and drier montane conditions of the western himalayas (altitudinal populations; 600-2202 m). in contrast with most other studies in drosophilids, d. hydei males exhibited comparatively higher desiccation resistance despite smaller body size compared to females. accordingly, we tested t ... | 2014 | 25045840 |
| local protein accumulation during gene activation. ii. interferometric measurements of the amount of solid material in temperature induced puffs of drosophila hydei. | 2014 | 4929853 | |
| y chromosome function and spermatogenesis in drosophila hydei. | 2013 | 3887859 | |
| effect of tin and lead chlorotriphenyl analogues on fruit fly drosophila hydei and liposomes membrane. | this article presents the results of a study investigating the biological activity of triphenyltin chloride (tpht) and two metalloorganic compounds, triphenyllead chloride (tphl) and triphenylmethane chloride (tphc), in their interaction with model membranes and the living organisms of fruit flies drosophila hydei. the study of model membranes (sonicated liposomes) was conducted using the electron spin resonance (esr) spin probe technique, whereas the experiment on fruit flies involved investiga ... | 2013 | 22447721 |
| permanent genetic resources added to molecular ecology resources database 1 april 2013-31 may 2013. | this article documents the addition of 234 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: acipenser sinensis, aleochara bilineata, aleochara bipustulata, barbus meridionalis, colossoma macropomum, delia radicum, drosophila nigrosparsa, fontainea picrosperma, helianthemum cinereum, liomys pictus, megabalanus azoricus, pelteobagrus vachelli, pleuragramma antarcticum, podarcis hispanica type 1a, sardinella brasiliensis and scle ... | 2013 | 23937578 |
| the embryonic and post-embryonic development in two drosophila species exposed to the static magnetic field of 60 mt. | in this study, a static magnetic field influence on development and viability in two different species, drosophila melanogaster and drosophila hydei, was investigated. both species completed development (egg-adult), in and out of the static magnetic field induced by double horseshoe magnet. treated vials with eggs were placed in the gap between magnetic poles (47 mm) and exposed to the average magnetic induction of 60 mt, while control ones were kept far enough from magnetic field source. we fou ... | 2011 | 21591895 |
| effect of the drosophila endosymbiont spiroplasma on parasitoid wasp development and on the reproductive fitness of wasp-attacked fly survivors. | in a previous study, we showed that spiroplasma, a maternally transmitted endosymbiotic bacterium of drosophila hydei, enhances larval to adult survival of its host when exposed to oviposition attack by the parasitoid wasp leptopilina heterotoma. the mechanism by which spiroplasma enhances host survival has not been elucidated. to better understand this mechanism, we compared the growth of wasp larvae in spiroplasma-infected and uninfected hosts. our results indicate that wasp embryos in spiropl ... | 2011 | 22912533 |
| evolution of y chromosomal lampbrush loop dna sequences of drosophila. | the evolutionary conservation of y chromosomal dna sequences of drosophila hydei in different species of the genus drosophila was studied by in situ hybridization and on genomic dna blots of restriction enzyme digested dna. we demonstrated that y specific dna sequences, which form major parts of lampbrush loops related to the male fertility genes, are only retained in a few closely related species during evolution. other y chromosomal dna sequences, also present in lampbrush loops but with homol ... | 2010 | 2881740 |
| spiroplasma bacteria enhance survival of drosophila hydei attacked by the parasitic wasp leptopilina heterotoma. | maternally-transmitted associations between endosymbiotic bacteria and insects are ubiquitous. while many of these associations are obligate and mutually beneficial, many are facultative, and the mechanism(s) by which these microbes persist in their host lineages remain elusive. inherited microbes with imperfect transmission are expected to be lost from their host lineages if no other mechanisms increase their persistence (i.e., host reproductive manipulation and/or fitness benefits to host). in ... | 2010 | 20730104 |
| mutation, recombination and genetic organization in drosophila hydei, compared to other drosophila species. | 2010 | 829893 | |
| microsatellite markers isolated from drosophila hydei. | we isolated and characterized 10 polymorphic microsatellite loci in drosophila hydei. the number of alleles per locus ranged from 3 to 8 (n = 23 individuals). polymorphic information content ranged from 0.316 to 0.750 and observed heterozygosity from 0.261 to 0.913. these markers will be valuable in studies of sexual selection and parental investment in d. hydei. | 2009 | 21564754 |
| drosophila melanogaster kl-3 and kl-5 y-loops harbor triple-stranded nucleic acids. | primary spermatocyte nuclei of drosophila melanogaster contain three prominent lampbrush-like loops. the development of these structures has been associated with the transcription of three fertility factors located on the y chromosome, named kl-5, kl-3 and ks-1. these loci have huge physical dimensions and contain extremely long introns. in addition, kl-3 and kl-5 were shown to encode two putative dynein subunits required for the correct assembly of the sperm axoneme. here, we show that both the ... | 2008 | 18430782 |
| negative effects of low temperatures on the vertical transmission and infection density of a spiroplasma endosymbiont in drosophila hydei. | maternally transmitted endosymbionts of the genus spiroplasma infecting several species of drosophila are known to cause selective death of male offspring (male killing). the male-killing trait is considered to be advantageous for maternally transmitted endosymbionts. however, a non-male-killing spiroplasma is present in japanese populations of drosophila hydei at high frequencies (23-66%). this spiroplasma is phylogenetically closely related to the male-killing spiroplasma infecting other droso ... | 2008 | 18618173 |
| spermatogenesis of drosophila hydei. | 2008 | 2289847 | |
| germline transgenesis and insertional mutagenesis in the ascidian ciona intestinalis. | stable transgenesis is a splendid technique that is applicable to the creation of useful marker lines, enhancer/gene traps, and insertional mutagenesis. recently, transposon-mediated transformation using a tc1/mariner transposable element minos has been reported in two ascidians: ciona intestinalis and c. savignyi. the transposon derived from an insect, drosophila hydei, has high activity for excision in ciona embryos and transposition in their genome. as much as 37% of minos-injected c. intesti ... | 2007 | 17342755 |
| on the phylogeny of the drosophila hydei subgroup: new insights from combined analyses of nuclear and mitochondrial data. | 2007 | 17292635 | |
| high positive predictive value of specific antibodies cross-reacting with a 28-kda drosophila antigen for diagnosis of ankylosing spondylitis. | diagnosis of ankylosing spondylitis (as) can be difficult, and a specific laboratory test has not yet been introduced as a routine diagnostic tool. our aim was to evaluate the diagnostic value of antibodies specifically binding to a recombinant 28-kda antigen for the diagnosis of as. | 2006 | 16159948 |
| prevalence of a non-male-killing spiroplasma in natural populations of drosophila hydei. | male-killing phenotypes are found in a variety of insects and are often associated with maternally inherited endosymbiotic bacteria. in several species of drosophila, male-killing endosymbionts of the genus spiroplasma have been found at low frequencies (0.1 to 3%). in this study, spiroplasma infection without causing male-killing was shown to be prevalent (23 to 66%) in japanese populations of drosophila hydei. molecular phylogenetic analyses showed that d. hydei was infected with a single stra ... | 2006 | 17021218 |
| replication patterns in polytene chromosomes of drosophila hydei, drosophila neohydei and their hybrids. | 2006 | 1053154 | |
| phylogeny and molecular evolution of the drosophila hydei subgroup (drosophila repleta group) inferred from the xanthine dehydrogenase gene. | the hydei subgroup (drosophila repleta group) consists of seven species divided into two complexes: bifurca and hydei, whose phylogenetic relationships are not well understood. to evaluate the molecular phylogeny of this subgroup, we analyzed 2085 bp of coding sequence of the xanthine dehydrogenase gene in six available species of the hydei subgroup, with drosophila buzzatii and drosophila mulleri as an outgroup. for phylogenetic reconstruction we adopted a maximum-likelihood framework, based on ... | 2005 | 15935705 |
| minos as a genetic and genomic tool in drosophila melanogaster. | much of the information about the function of d. melanogaster genes has come from p-element mutagenesis. the major drawback of the p element, however, is its strong bias for insertion into some genes (hotspots) and against insertion into others (coldspots). within genes, 5'-utrs are preferential targets. for the successful completion of the drosophila genome disruption project, the use of transposon vectors other than p will be necessary. we examined here the suitability of the minos element fro ... | 2005 | 15972463 |
| transposition of the drosophila hydei minos transposon in the mouse germ line. | we tested the suitability of the fly transposon minos, a member of the tc1/mariner superfamily, for insertional mutagenesis in the mouse germ line. we generated a transgenic mouse line expressing minos transposase in growing oocytes and another carrying a tandem array of nonautonomous transposons. the frequency of transposition in the progeny derived from oocytes carrying both transgenes is 8.2%. analysis of the new integration sites shows a high frequency of transpositions to a different chromo ... | 2003 | 12620388 |
| transposable elements as tools for genomics and genetics in drosophila. | the p-element has been the workhorse of drosophila genetics since it was developed as a tool for transgenesis in 1982; the subsequent development of a variety of systems based on the transposon have provided a range of powerful and flexible tools for genetics and genomics applications. p-element insertions are frequently used as starting-points for generating chromosomal deletions to remove flanking genes, either by screening for imprecise excision events or by selecting for male recombination e ... | 2003 | 15239944 |
| indiscriminate females and choosy males: within- and between-species variation in drosophila. | the classic view of choosy, passive females and indiscriminate, competitive males gained theoretical foundations with parental investment theory. when females invest more in offspring than males, parental investment theory says that selection operates so that females discriminate among males for mates (i.e., females are choosy and passive) and males are indiscriminate (i.e., males are profligate and competitive). here we report tests of predictions using drosophila pseudoobscura and d. melanogas ... | 2003 | 14575325 |
| magnesium transport through the basal plasma membrane of larval malpighian tubules of drosophila hydei studied by electron probe x-ray microanalysis. | magnesium besides calcium is the most important excretion product. in the anterior malpighian tubules of drosophila, excretion of magnesium takes place via the hindgut by proteoglycan containing concretions. this study reports on magnesium transport through the basal plasma membrane of the principal cells of the proximal segment of the anterior malpighian tubules. measurements by electron probe x-ray microanalysis indicate the existence of two antiporters which transfer magnesium in still unknow ... | 2002 | 12030418 |
| in vivo transposition of minos, a drosophila mobile element, in mammalian tissues. | transposable elements have been used widely in the past 20 years for gene transfer and insertional mutagenesis in drosophila. transposon-based technology for gene manipulation and genomic analysis currently is being adopted for vertebrates. we tested the ability of minos, a dna transposon from drosophila hydei, to transpose in mouse tissues. two transgenic mouse lines were crossed, one expressing minos transposase in lymphocytes under the control of the cd2 promoter/locus control region and anot ... | 2001 | 11562481 |
| identification of some heat-induced genes of trichinella spiralis. | three heat-induced genes of the infective-stage larvae of trichinella spiralis were successfully identified by the suppression subtractive hybridization (ssh) technique. as indicated by reverse northern blotting, 19 of 25 clones were scored as differentially transcribed in the heat-shocked infective-stage larvae. the sequencing data showed the presence of 12 different genes. three were homologous to histone h3, histone h2b and translationally controlled tumour protein (tctp). a 0.6 kb cdna of hi ... | 2001 | 11578093 |
| the endogenous drosophila melanogaster retrovirus gypsy can propagate in drosophila hydei cells. | the endogenous drosophila melanogaster retrovirus gypsy (mdg4) forms virus-like particles (vlps) which are found as extracellular particles in the medium used to culture d. melanogaster cells. the d. hydei somatic cell line dh14, which does not harbour gypsy sequences, was exposed to d. melanogaster vlps. subsequent pcr and southern analysis revealed that gypsy elements had penetrated into the d. hydei cells, suggesting interspecific transmission of the retrovirus. a d. hydei cell line containin ... | 2001 | 11212913 |
| evolution of gypsy endogenous retrovirus in the drosophila obscura species group. | the ty3/gypsy family of retroelements is closely related to retroviruses, and some of their members have an open reading frame resembling the retroviral gene env. sequences homologous to the gypsy element from drosophila melanogaster are widely distributed among drosophila species. in this work, we report a phylogenetic study based mainly on the analysis of the 5' region of the env gene from several species of the obscura group, and also from sequences already reported of d. melanogaster, drosop ... | 2000 | 10908638 |
| evolutionary change of codon usage for the histone gene family in drosophila melanogaster and drosophila hydei. | the nucleotide divergence in the protein-coding region for replication-dependent and replication-independent histone 3 and 4 genes of drosophila melanogaster and drosophila hydei occurred mostly at the synonymous site. therefore, the pattern of codon usage was analyzed in the two species, considering the genomic codon bias, which is proposed for estimating the genomic composition pressure in the protein-coding regions. the results indicated that the codon usage in the histone gene family could b ... | 2000 | 10837157 |
| genome-wide insertional mutagenesis in human cells by the drosophila mobile element minos. | the development of efficient non-viral methodologies for genome-wide insertional mutagenesis and gene tagging in mammalian cells is highly desirable for functional genomic analysis. here we describe transposon mediated mutagenesis (tramm), using naked dna vectors based on the drosophila hydei transposable element minos. by simple transfections of plasmid minos vectors in hela cells, we have achieved high frequency generation of cell lines, each containing one or more stable chromosomal integrati ... | 2000 | 11258481 |
| molecular evolution and phylogeny of the buzzatii complex (drosophila repleta group): a maximum-likelihood approach. | the buzzatii complex of the mulleri subgroup (drosophila repleta group) consists of three clusters of species whose evolutionary relationships are poorly known. we analyzed 2,085 coding nucleotides from the xanthine dehydrogenase (xdh:) gene in the 10 available species of the complex and drosophila mulleri and drosophila hydei. we adopted a statistical model-fitting approach within the maximum-likelihood (ml) framework of phylogenetic inference. we first modeled the process of nucleotide substit ... | 2000 | 10889224 |
| correlated response in reproductive and life history traits to selection on testis length in drosophila hydei. | flies in the genus drosophila have undergone striking evolutionary divergence in the size and number of sperm produced. based on comparative studies of sperm length, testis length, and other reproductive and life history traits, including body size, age at first reproduction, and the number of sperm produced, macroevolutionary trade-offs resulting from the need to produce high-investment testes have been postulated. to understand better the microevolutionary processes underlying these interspeci ... | 2000 | 10849065 |
| mega-introns in the dynein gene dhdhc7(y) on the heterochromatic y chromosome give rise to the giant threads loops in primary spermatocytes of drosophila hydei. | the heterochromatic y chromosomes of several drosophila species harbor a small number of male fertility genes (fertility factors) with several unusual features. expression of their megabase-sized loci is restricted to primary spermatocytes and correlates with the unfolding of species-specific lampbrush loop-like structures resulting from huge transcripts mainly derived from clusters of loop-specific y chromosomal satellites. otherwise, there is evidence from genetic mapping and biochemical exper ... | 2000 | 10655227 |
| functional analysis of an eye specific enhancer of the eyeless gene in drosophila. | the development of the drosophila compound eye requires the function of a set of evolutionarily conserved genes. among these, the drosophila pax-6 gene eyeless (ey) plays a major role. ey has been considered a master control gene of eye development in the animal kingdom because targeted expression of ey and vertebrate as well as invertebrate homologs lead to the formation of ectopic eyes in drosophila. we demonstrate that an intron of the ey gene contains an enhancer that regulates the eye speci ... | 1999 | 9892673 |
| proteins with tandemly arranged repeats of a highly charged 16-amino-acid motif encoded by the dhmst101 gene family are structural components of the outer sheath of the extremely elongated sperm tails of drosophila hydei. | fruit fly species of the genus drosophila show a remarkable variation in sperm length. some of them produce gigantic sperm several times the total male body length. sperm of drosophila hydei, for example, are more than 20 mm long. little is known about the advantage of such elongated sperm or about the proteins that stabilize their thin flagellar tails. recently, two members of a novel gene family dhmst101(1) and dhmst101(2), whose gene products are associated with the sperm tail, were isolated ... | 1999 | 10445506 |
| the y chromosomal fertility factor threads in drosophila hydei harbors a functional gene encoding an axonemal dynein beta heavy chain protein. | to understand the contradiction between megabase-sized lampbrush loops and putative protein encoding genes both associated with the loci of y chromosomal fertility genes of drosophila on the molecular level, we used pcr-mediated cloning to identify and isolate the cdna sequence of the y chromosomal drosophila hydei gene dhdhc7(y). alignment of the sequences of the putative protein dhdhc7(y) and the outer arm dynein beta heavy chain protein dyh2 of tripneustes gratilla shows homology over the ent ... | 1998 | 9649526 |
| a regulatory element of the empty spiracles homeobox gene is composed of three distinct conserved regions that bind regulatory proteins. | homeobox genes encode a class of highly evolutionarily conserved transcription factors that control embryonic development. the drosophila melanogaster empty spiracles gene is the homolog of the two human homeobox genes emx1 and emx2. these genes are necessary for central nervous system development. we used a regulatory element of the empty spiracles gene to study the control of homeobox gene expression. the 1.2-kilobase (kb) cis-regulatory element located 3 kb 5' of the transcription start site ... | 1998 | 9491376 |
| molecular cloning and characterization of a transcription factor for the copia retrotransposon with homology to the btb-containing lola neurogenic factor. | by transfection experiments, we previously identified a 72-bp enhancer sequence within the drosophila copia retrotransposon which is involved in the control of the transcription level of this mobile element in cells in culture. gel shift assays with nuclear extracts from drosophila hydei-derived dh-33 cells further demonstrated specific interactions of at least two nuclear factors with this enhancer sequence. using this sequence as a probe for the screening of an expression cdna library that we ... | 1997 | 8972229 |
| naturally occurring testis-specific histone h3 antisense transcripts in drosophila. | while analysing the transcription of the cluster of cell-cycle regulated histone genes in drosophila hydei, we have found transcripts spanned both histone h3 and h4 genes and were antisense for histone h3. as the two histone genes are in opposite orientation, these transcripts contained the sense strand of the histone h4 gene. such transcripts were present in both poly(a)+ and poly(a)- rna fractions. the polyadenylated molecules contained a poly(a) tail at the 3' end of the stem-loop structure, ... | 1997 | 9364435 |
| molecular cloning of a family of xenobiotic-inducible drosophilid cytochrome p450s: evidence for involvement in host-plant allelochemical resistance. | cytochrome p450s constitute a superfamily of genes encoding mostly microsomal hemoproteins that play a dominant role in the metabolism of a wide variety of both endogenous and foreign compounds. in insects, xenobiotic metabolism (i.e., metabolism of insecticides and toxic natural plant compounds) is known to involve members of the cyp6 family of cytochrome p450s. use of a 3' race (rapid amplification of cdna ends) strategy with a degenerate primer based on the conserved cytochrome p450 heme-bind ... | 1997 | 9380713 |
| a graphical method for analysing the dynamics of three-species systems. | we report a series of experiments with three competing species and a novel graphical analysis that explores the dynamics of this simple multi-species system. the three competing species (drosophila hydei, d. immigrans and d. virilis) were maintained in five very large cages, on a natural fruit resource. the analytic method involved constructing a "dynamic surface", by interpolation, from the population trajectories. within the dynamic surface constructed from the five experiments a small "equili ... | 1997 | 28308114 |
| cis-dichloro(alpha,omega-diamino carboxylate ethyl ester)palladium(ii) as palladium(ii) versus platinum(ii) model anticancer drugs: synthesis, solution equilibria of their aqua, hydroxo, and/or chloro species, and in vitro/in vivo dna-binding properties. | cis-dichloro(d,l-2,3-diaminopropionate ethyl ester)palladium(ii), cis-[pd(etdap)cl(2)] (i), and cis-dichloro(d,l-2,4-diaminobutyrate ethyl ester)palladium(ii), cis-[pd(etdab)cl(2)] (ii), were synthesized and characterized by elemental analysis, ir spectroscopy, and tg-dta thermal analysis. the equilibrium model and log beta(pqr) constants of hydrolytic species of i and ii were also investigated by potentiometric methods (i = 0.15 m (naclo(4)) and 37 degrees c). aqueous solutions of cis-[pd(etdaa ... | 1997 | 11669784 |
| fluorescence in situ hybridization of ribosomal dna to mitotic chromosomes of tsetse flies (diptera: glossinidae: glossina). | ribosomal genes were mapped in glossina austeni, g. brevipalpis, g. f. fuscipes, g. m. submorsitans, g. p. palpalis, g. pallidipes and g. tachinoides on mitotic chromosomes by fluorescence in situ hybridization (fish) using drosophila hydei genomic clones that contain the 28s ribosomal dna. in all species except g. brevipalpis, the ribosomal genes were located on the long arm of autosome l1. the y chromosomes of g. pallidipes and g. p. palpalis showed additional hybridization signals. supernumer ... | 1997 | 9244454 |
| identification of the sex-determining region of the ceratitis capitata y chromosome by deletion mapping. | in the medfly ceratitis capitata, the y chromosome is responsible for determining the male sex. we have mapped the region containing the relevant factor through the analysis of y-autosome translocations using fluorescence in situ hybridization with two different probes. one probe, the clone py114, contains repetitive, y-specific dna sequences from c. capitata, while the second clone, pdh2-h8, consists of ribosomal dna sequences from drosophila hydei. clone py114 labeled most of the long arm and ... | 1996 | 8889534 |
| molecular systematics of the drosophila hydei subgroup as inferred from mitochondrial dna sequences. | the phylogeny of the drosophila hydei subgroup, which is a member of the d. repleta species group, was inferred from 1,515 base pairs of mitochondrial dna sequence of the cytochrome oxidase subunits i, ii, and iii. four of the seven species in the subgroup were examined, which are placed into two taxonomic complexes: the d. bifurca complex (d. bifurca and d. nigrohydei) and the d. hydei complex (d. hydei and d. eohydei). both complexes appear to be monophyletic, although the d. bifurca complex i ... | 1996 | 8703094 |
| transcription of gypsy elements in a y-chromosome male fertility gene of drosophila hydei. | we have found that defective gypsy retrotransposons are a major constituent of the lampbrush loop pair nooses in the short arm of the y chromosome of drosophila hydei. the loop pair is formed by male fertility gene q during the primary spermatocyte stage of spermatogenesis, each loop being a single transcription unit with an estimated length of 260 kb. using fluorescent in situ hybridization, we show that throughout the loop transcripts gypsy elements are interspersed with blocks of a tandemly r ... | 1996 | 8852843 |
| strukturdifferenzierungen in y-chromosom von drosophila hydei: the unique morphology of the y chromosomal lampbrush loops threads results from 'coaxial shells' formed by different satellite-specific subregions within megabase-sized transcripts. | the results of pulsed-field gel electrophoresis (pfge) analysis and two-colour transcript fluorescence in situ hybridization (fish) for the three threads-specific dna satellites ylii, yli and rally are in support of long-range clustering of these sequence families within the subterminal region on the long arm of the y chromosome of drosophila hydei. on the basis of the linear arrangement of at least four extended clusters of satellite-specific sequences, the loop morphology of wild-type and seve ... | 1996 | 8785614 |
| molecular phylogeny and genome evolution in the drosophila virilis species group: duplications of the alcohol dehydrogenase gene. | drosophila virilis is a prominent reference species for comparison with drosophila melanogaster in regard to patterns and mechanisms of molecular and genomic evolution. sequences were determined for 11 adh genes from 8 species of the d. virilis species group, including species from both the virilis phylad and the montana subphylad. the genome of d. virilis contains a 6-kb duplication that includes the entire adh coding region. the pattern of sequence identity within the duplication strongly sugg ... | 1996 | 8583887 |
| gene transfer into the medfly, ceratitis capitata, with a drosophila hydei transposable element. | exogenous functional dna was introduced into the germline chromosomes of the mediterranean fruit fly (medfly) ceratitis capitata with a germline transformation system based on the transposable element minos from drosophila hydei. transformants were identified as phenotypic revertants of a white-eyed mutation carried by the recipient strain. clusters of transformants were detected among the progeny of 390 individuals screened for germline transformation. five independent and phenotypically active ... | 1995 | 8533094 |
| structure and regulation of the fushi tarazu gene from drosophila hydei. | the drosophila melanogaster segmentation gene fushi tarazu (ftz) encodes a homeodomain-type transcription factor involved in the control of larval pattern formation. loss of function mutations cause an embryonic lethal, pair-rule phenotype. the segmentation defects, but not the lethality, can be partially rescued by the ftz orthologue from drosophila hydei. in this work, the primary structure, expression and regulation of the d. hydei ftz gene was characterized. sequence comparisons classify ftz ... | 1995 | 28306077 |
| introduction of the transposable element minos into the germ line of drosophila melanogaster. | a transposon based on the transposable element minos from drosophila hydei was introduced into the genome of drosophila melanogaster using transformation mediated by the minos transposase. the transposon carries a wild-type version of the white gene (w) of drosophila inserted into the second exon of minos. transformation was obtained by injecting the transposon into preblastoderm embryos that were expressing transposase either from a hsp70-minos fusion inserted into the genome via p-element-medi ... | 1995 | 7568159 |
| structure and expression of histone h3.3 genes in drosophila melanogaster and drosophila hydei. | we demonstrate that in drosophila melanogaster the histone h3.3 replacement variant is encoded by two genes, h3.3a and h3.3b. we have isolated cdna clones for h3.3a and cdna and genomic clones for h3.3b. the genes encode exactly the same protein but are widely divergent in their untranslated regions (utr). both genes are expressed in embryos and adults; they are expressed in the gonads as well as in somatic tissues of the flies. however, only one of them, h3.3a, shows strong testes expression. t ... | 1995 | 7557364 |
| minor-myosin, a novel myosin isoform synthesized preferentially in drosophila testis is encoded by the muscle myosin heavy chain gene. | searching for structural proteins involved in spermatogenesis of drosophila, we found a novel myosin isoform in the testis of drosophila hydei and d. melanogaster. the transcript encoding this isoform, which we called 'minor-myosin', initiates within the intron between exons 12 and 13 of the muscle myosin heavy chain (mmhc) gene. minor-myosin contains a common myosin tail but no ordinary myosin head domain. instead, it has a short n-terminal domain which displays similarity with the n-termini of ... | 1995 | 7669694 |
| [unusually prolonged extrachromosomal stability of plasmids containing fragments of retrotransposon mdg4, after transfection of drosophila hydei cell cultures]. | 1995 | 7735112 | |
| tandemly arranged repeats of a novel highly charged 16-amino-acid motif representing the major component of the sperm-tail-specific axoneme-associated protein family dhmst101 form extended alpha-helical rods within the extremely elongated spermatozoa of drosophila hydei. | we have previously reported that the sperm-tail-specific proteins in drosophila hydei, encoded by the small dhmst101 gene family, contained several tandem repeats of a novel highly charged, well-conserved cysteine-containing motif of 16 amino acids, kkkcaeaakkekeaae [neesen, j., bünemann, h. & heinlein, u. a. o. (1994) dev. biol. 162, 414-425] and suggested that this motif might be important in the structural and functional integrity of the sperm tail. we tested this suggestion by examining stru ... | 1994 | 7957199 |
| degenerating gypsy retrotransposons in a male fertility gene on the y chromosome of drosophila hydei. | during the evolution of the y chromosome of drosophila hydei, retrotransposons became incorporated into the lampbrush loop pairs formed by several of the male fertility genes on this chromosome. although insertions of retrotransposons are involved in many spontaneous mutations, they do not affect the functions of these genes. we have sequenced gypsy elements that are expressed as constituents of male fertility gene q in the lampbrush loop pair nooses. we find that these gypsy elements are all tr ... | 1994 | 7807535 |
| another way of being anisogamous in drosophila subgenus species: giant sperm, one-to-one gamete ratio, and high zygote provisioning. | it is generally assumed that sexes in animals have arisen from a productivity versus provisioning conflict; males are those individuals producing gametes necessarily small, in excess, and individually bereft of all paternity assurance. a 1- to 2-cm sperm, 5-10 times as long as the male body, might therefore appear an evolutionary paradox. as a matter of fact, species of drosophila of the drosophila subgenus differ from those of other subgenera by producing exclusively sperm of that sort. we repo ... | 1994 | 7937962 |
| transcription of repetitive dna sequences in the lampbrush loop pair nooses formed by sterile alleles of fertility gene q on the y chromosome of drosophila hydei. | the y chromosomal lampbrush loop-forming male fertility genes of drosophila consist mainly of repetitive dna sequences that do not code for proteins. we investigated whether differences in the transcription of these sequences can be detected in male-sterile alleles of male fertility gene q, which forms the loop pair nooses. the loop consists, for approximately two-thirds, of repeats of the y-specific ay1 family of repetitive dna sequences. of the remaining one-third, at least one-half is represe ... | 1994 | 7969035 |
| representative and efficient cloning of satellite dnas based on pfge pre-fractionation of restriction digests of genomic dna. | using dna from drosophila hydei kun-dh-33 cells we describe an efficient method for selective and representative cloning of complex mixtures of satellite dnas from eukaryotic genomes. effective separation of satellite dna from the bulk of all other sequences it obtained by fractionation of high molecular weight dna by pfge after treating it with '6 bp' restriction enzymes. since extended clusters of tandemly arranged, so called simple sequence, repeats are inert to cleavage by most '6 bp' restri ... | 1994 | 7963251 |
| the drosophila hydei gene dhmst101(1) encodes a testis-specific, repetitive, axoneme-associated protein with differential abundance in y chromosomal deletion mutant flies. | to understand the effect of the megabase-sized, y chromosomal fertility genes on different stages of spermatogenesis in drosophila hydei, an immunoscreening was performed to search for testis-specific protein-encoding cdnas. the array of isolated clones contained cdna sequences derived from a gene on chromosome 5 at 101bc. the gene, dhmst101(1), is a member of a small gene family and is specifically expressed in adult testis tissue. the mrna encodes a protein of 344 amino acids with a deduced ap ... | 1994 | 8150205 |
| interspecific sequence comparison of the muscle-myosin heavy-chain genes from drosophila hydei and drosophila melanogaster. | the muscle-myosin heavy-chain (mmhc) gene of drosophila hydei has been sequenced completely (size 23.3 kb). the sequence comparison with the d. melanogaster mmhc gene revealed that the exon-intron pattern is identical. the protein coding regions show a high degree of conservation (97%). the alternatively spliced exons (3a-b, 7a-d, 9a-c, 11a-e, and 15a-b) display more variations in the number of nonsynonymous and synonymous substitutions than the common exons (2, 4, 5, 6, 8, 10, 12, 13, 14, 16, 1 ... | 1994 | 7966366 |
| the drosophila micropia retrotransposon encodes a testis-specific antisense rna complementary to reverse transcriptase. | the micropia transposable element of drosophila hydei is a long terminal repeat-containing retrotransposon present in both the autosomes and the y chromosome. micropia expression gives rise to a complex set of sense and antisense rnas transcribed primarily during spermatogenesis. the most abundant sense rnas constitute an assortment of heterogeneous high-molecular-weight transcripts expressed as constituents of the y-chromosomal lampbrush loops of primary spermatocytes. in addition, micropia enc ... | 1994 | 7509447 |
| large-male advantages associated with costs of sperm production in drosophila hydei, a species with giant sperm. | males of the fruit fly drosophila hydei were found to produce 23.47 +/- 0.46-mm-long spermatozoa, the longest ever described. no relationship was found between male body size and sperm length. we predicted that if these giant gametes are costly for males to produce, then correlations should exist between male body size, rates of sperm production, and fitness attributes associated with the production of sperm. smaller males were found to make a greater relative investment in testicular tissue gro ... | 1994 | 7937755 |
| identification of a strong transcriptional activator for the copia retrotransposon responsible for its differential expression in drosophila hydei and melanogaster cell lines. | we have characterized the regulatory properties of a 72bp sequence located in the 5' untranslated domain of the drosophila copia retrotransposon, 3' to the left ltr, by transient transfection assays with cell lines derived from either drosophila hydei (dh33 cells) or drosophila melanogaster (schneider ii and kc cells). reporter plasmids were constructed which contained the lacz gene under the control of either the entire copia ltr with 5' untranslated domain, or a minimal heterologous promoter f ... | 1994 | 8074683 |
| mobile minos elements from drosophila hydei encode a two-exon transposase with similarity to the paired dna-binding domain. | elements related to the tc1-like minos mobile element have been cloned from drosophila hydei and sequenced. southern blot and sequence analyses show that (i) the elements are actively transposing in the drosophila hydei germ line, (ii) they are characterized by a striking degree of sequence and size homogeneity, and (iii) like tc1, they insert at a ta dinucleotide that is probably duplicated during the process. the nucleotide sequences of two elements, minos-2 and minos-3, differ at only one pos ... | 1994 | 8197129 |
| evolutionary conservation of the structure and expression of alternatively spliced ultrabithorax isoforms from drosophila. | in drosophila melanogaster, alternatively spliced mrnas from the homeotic gene ultrabithorax (ubx) encode a family of structurally distinct homeoprotein isoforms. the developmentally regulated expression patterns of these isoforms suggest that they have specialized stage- and tissue-specific functions. to evaluate the functional importance of ubx isoform diversity and gain clues to the mechanism that regulates processing of ubx rnas, we have investigated whether the ubx rnas of other insects und ... | 1994 | 7911773 |
| discrimination of related transcribed and non-transcribed repetitive dna sequences from the y chromosomes of drosophila hydei and drosophila eohydei. | the short arm of the y chromosome of drosophila hydei carries a single male fertility gene, gene q, which forms the lampbrush loop pair nooses. conflicting observations have been reported concerning the identity of the repetitive dna sequences that are transcribed in this loop pair. it has been claimed by other investigators that the loop transcripts contain repeats of two distinct, but related families of y-specific repetitive dna sequences, ay1 and ysi. we reinvestigated this issue, using as p ... | 1994 | 8190071 |
| hsp90 associates with specific heat shock puffs (hsr omega) in polytene chromosomes of drosophila and chironomus. | the heat shock protein hsp90, which is mainly cytoplasmic, has recently been reported to be present in the nucleus. we have found a specific chromosomal localization of hsp90 in different species of drosophila and chironomus using immunocytochemical techniques with different mono- and polyclonal antibodies for this hsp. hsp90 was found associated with heat shock-induced puffs at 93d and 48b in salivary gland chromosomes of drosophila melanogaster and drosophila hydei, respectively. the localizat ... | 1993 | 8306827 |
| an 18-bp element in the 5' untranslated region of the drosophila beta 2 tubulin mrna regulates the mrna level during postmeiotic stages of spermatogenesis. | during drosophila spermatogenesis transcriptional activity is mainly restricted to premeiotic stages. translation during sperm morphogenesis, however, proceeds for several days, requiring a high stability for mrnas translated postmeiotically. we studied expression of the drosophila beta 2 tubulin gene, which is expressed solely in the male germ line from the primary spermatocyte stage onwards. cis-acting elements involved in the regulation of mrna levels were investigated in transgenic fly strai ... | 1993 | 8269980 |
| the anti z-dna reactivity of z-dna forming sequences is affected by platinum antitumor drugs. | the effect of binding of platinum antitumor drugs, cis-diamminedichloroplatinum (ii) (cis-ddp) and pt-pentamidine, on the z-dna reactivity of potential z-dna forming sequences has been studied by enzyme-linked immunosorbent assay. the results indicate that cis-ddp and pt-pentamidine increase the z-dna reactivity of plasmids containing a (dg-dc)16 insert (pucz8) and a native z-dna forming sequence of drosophila hydei (pf18). the molar ratio of platinum bound to nucleotides (rb) to produce 50% of ... | 1993 | 8227037 |
| a telomere-like satellite (gggtcat)n comprises 4% of genomic dna of drosophila hydei and is located mainly in centromeric heterochromatin of all large acrocentric autosomes. | we describe the isolation and analysis of a g+c-rich telomere-like (gggtcat)n satellite of drosophila hydei. gggtcat-specific clones were obtained by a combination of restriction enzyme digestion and fractionation of d. hydei dna by pulsed-field gel electrophoresis (pfge). high-molecular-weight dna (> 50 kb) was recovered from gels, sheared by sonication and cloned into the pbs plasmid vector. the results of pfge analysis, quantitative dot blot measurements and fluorescence in situ hybridization ... | 1993 | 8299961 |
| localization of the lampbrush loop pair nooses on the y chromosome of drosophila hydei by fluorescence in situ hybridization. | we have used fluorescence in situ hybridization to map the positions of the different repetitive dna sequences from the region forming the lampbrush loop pair nooses on the y chromosome of drosophila hydei. this region harbours a megabase cluster of tandemly organized repeats of the y-specific ay1 family and a megabase cluster of tandem repeats of the related y-specific ysi family. in addition, ay1 repeats also occur in short blocks that are interspersed by other repetitive dna sequences that we ... | 1993 | 8243166 |
| partial reconstruction of the lampbrush loop pair nooses on the y chromosome of drosophila hydei. | we present the analysis of genomic dna fragments that were isolated as potential segments of the lampbrush loop pair nooses on the short arm of the y chromosome of drosophila hydei. more than 300 kb of dna were recovered in bamhi lambda and cosmid clone groups. this dna is composed of the y-specific ay1 family of repetitive dna sequences, and of other repetitive dna sequences, which at least in part are also located elsewhere in the genome (y-associated sequences). two additional classes of dna ... | 1993 | 8243165 |
| genetic heterogeneity increases viability in competing groups of drosophila hydei. | 1993 | 28567895 | |
| heterogeneous distribution of elemental contents in the larval malpighian tubules of drosophila hydei: x-ray microanalysis of freeze-dried cryosections. | the elements na, cl and k are distributed heterogeneously in the larval cells of the four malpighian tubules of drosophila hydei. elemental gradients are steeper when they are related to the dry weight in comparison to the weight of water. the high contents of na, cl and k in the cytoplasm close to the basal plasma membrane differ significantly from the low values found in the neighboring basal "labyrinth" and in the intermediate cytoplasm. elemental contents (k, cl) increase from the intermedia ... | 1993 | 8339320 |
| low codon bias and high rates of synonymous substitution in drosophila hydei and d. melanogaster histone genes. | we have evaluated codon usage bias in drosophila histone genes and have obtained the nucleotide sequence of a 5,161-bp d. hydei histone gene repeat unit. this repeat contains genes for all five histone proteins (h1, h2a, h2b, h3, and h4) and differs from the previously reported one by a second ecori site. these d. hydei repeats have been aligned to each other and to the 5.0-kb (i.e., long) and 4.8-kb (i.e., short) histone repeat types from d. melanogaster. in each species, base composition at sy ... | 1993 | 8487638 |
| towards a physical map of the fertility genes on the heterochromatic y chromosome of drosophila hydei: families of repetitive sequences transcribed on the lampbrush loops nooses and threads are organized in extended clusters of several hundred kilobases. | the understanding of structure and function of the so-called fertility genes of drosophila is very limited due to their unusual size--several megabases--and their location on the heterochromatic y chromosome. since mapping of these genes has mainly been done by classical cytogenetic analyses using a small number of cytologically visible lampbrush loops as the sole markers for particular fertility genes, the resolution of the genetic map of the y chromosome is restricted to 3-5 mb. here we demons ... | 1992 | 1465096 |
| constructing balancer chromosomes for genetic screens in drosophila hydei. | we used a screen for maternally generated late embryonic lethals as a new method for the isolation of inversions that are suitable for the balancing of mutations in drosophila hydei. the recovery of several inversions by this method demonstrates that female meiosis in d. hydei apparently differs from meiosis in female d. melanogaster, since in d. hydei the defective chromosomes which are generated by a single crossing-over within a paracentric inversion can be recovered via the egg nucleus. in a ... | 1992 | 24202759 |
| characterization of the reverse transcriptase of 1731, a drosophila melanogaster retrotransposon. | the nucleotide sequence of 1731, a retrotransposon cloned from the genome of drosophila melanogaster, reveals a structural similarity with the proviral form of the retroviruses including a pol-like gene containing a putative reverse-transcriptase(rt)-coding sequence. diverse parts of that sequence were subcloned and expressed in escherichia coli. it has been demonstrated that the expression of the rt-like sequence, when translated, gives rise to peptides displaying enzyme activity characteristic ... | 1992 | 1385119 |
| laminin in the male germ cells of drosophila. | to study genes that may be crucial for the male germ cell development of drosophila we screened a cdna expression library with a polyclonal antiserum against testis proteins of drosophila hydei. we identified a cdna fragment that exhibited a complete sequence similarity with the cdna of the laminin b2 chain, an important component of the extracellular matrix. transcripts of laminin b2 were detected in the rna of male germ cells with the polymerase chain reaction and by in situ hybridization. we ... | 1992 | 1429843 |
| microdeletions in interval 6 of the y chromosome of males with idiopathic sterility point to disruption of azf, a human spermatogenesis gene. | for males with idiopathic sterility, a molecular screen specific for small lesions (microdeletions) in interval 6 of the y chromosome was set up using 29 y-dna probes. a "de novo" microdeletion in y interval 6 was detected in 2 out of 19 "chromosomally normal" sterile males. the first microdeletion includes the y-dna probes py6hp35 and 12f3; the second microdeletion includes the y-dna probes py6hp52, 49f, fr15-ii and the subinterval "c" of probe 50f2. a probe of the py6h sequence family is prese ... | 1992 | 1634226 |
| the evolution of duplicate glyceraldehyde-3-phosphate dehydrogenase genes in drosophila. | in drosophila melanogaster there are two genes which encode the enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh), gapdh-43e and gapdh-13f. we have shown that gapdh-43e codes for the gapdh subunit with an apparently larger molecular weight while gapdh-13f encodes the gapdh subunit having an apparently smaller molecular weight. immunoblots of sodium dodecyl sulfate gels were used to survey species from throughout the genus and results indicated that two classes of gapdh subunits are present ... | 1992 | 1468631 |
| interspecific comparison of the transformer gene of drosophila reveals an unusually high degree of evolutionary divergence. | the transformer (tra) gene of drosophila melanogaster occupies an intermediate position in the regulatory pathway controlling all aspects of somatic sexual differentiation. the female-specific expression of this gene's function is regulated by the sex lethal (sxl) gene, through a mechanism involving sex-specific alternative splicing of tra pre-mrna. the tra gene encodes a protein that is thought to act in conjunction with the transformer-2 (tra-2) gene product to control the sex-specific process ... | 1992 | 1592233 |