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specificity of mass spectrometry (maldi-tof) in the diagnosis of babesia canis regarding to other canine vector-borne diseases.the canine vector-borne diseases (cvbd) is a term, which describes a range of infectious or/and parasitic diseases whose etiological agents are transmitted by vectors. cvbd are becoming more widely in the world in relation to global warming and the increasing number of infected vectors. the aim of this study was to assess rapid mass spectrometry (ms) - based proteomics analyses for diagnosis of babesia canis, anaplasma phagocytophilum and borrelia burgdorferi infections in dogs. the study was co ...201727614473
species of ticks and carried pathogens in owned dogs in spain: results of a one-year national survey.this study presents the results of a national survey in spain on the distribution of ticks on owned dogs, their phenology and the associated pathogens over one year. in the study, 1628 adult ticks were collected on 660 dogs presented to 26 veterinary practices, of which 507 dogs (76.8%) carried at least one adult tick. the primary species of ticks were rhipicephalus sanguineus s.l. (53%), dermacentor reticulatus (9%), ixodes ricinus (9%), and i. hexagonus (4%). parasitism by two species of these ...201728188085
first molecular detection of tick-borne pathogens in dogs from jiangxi, china.in this study, blood samples obtained from 162 dogs in jiangxi, china, were employed in molecular screening of canine tick-borne pathogens by pcr and sequencing. babesia spp. gene fragment was detected in 12 (7.41%) dogs. all samples were negative for hepatozoon spp., ehrlichia canis, coxiella spp., borrelia spp., rickettsia spp. and anaplasma platys. species-specific pcr analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by babesia canis vogeli and babesia gibsoni, respe ...201727890889
horses infected by piroplasms different from babesia caballi and theileria equi: species identification and risk factors analysis in italy.equine piroplasmosis (ep) caused by theileria equi and babesia caballi is a disease affecting the health and the international movement of horses. in order to assess prevalence of piroplasmid infection in the northwestern part of italy and to evaluate the associated risk factors, whole blood was collected from 135 horses from 7 different stables across the study area. pcr and sequencing were used to assess prevalence of infection and to identify detected piroplasms to species level. a total of 2 ...201728288762
the prevalence and impact of babesia canis and theileria sp. in free-ranging grey wolf (canis lupus) populations in croatia.babesia spp. and theileria spp. are important emerging causes of disease in dogs. alongside these domesticated hosts, there is increasing recognition that these piroplasms can also be found in a range of wild animals with isolated reports describing the presence of these pathogen in foxes (vulpes vulpes) and captive grey wolves (canis lupus). the prevalence and impact of these infections in free-ranging populations of canids are unknown. to gain a better insight into the epidemiology and pathoge ...201728376903
canine tick-borne diseases in pet dogs from romania.tick-borne diseases are of substantial concern worldwide for animals as well as humans. dogs have been a human companion for millennia, and their significant impact on human life renders disease in dogs to be of great concern. tick-borne diseases in dogs represent a substantial diagnostic challenge for veterinarians in that clinical signs are often diffuse and overlapping. in addition, co-infections with two or more pathogens enhance this problem further. molecular methods are useful to disentan ...201728335825
origins of recently emerged foci of the tick dermacentor reticulatus in central europe inferred from molecular markers.the ornate dog tick dermacentor reticulatus is vector of several blood parasites, including babesia canis, a causative agent of babesiosis. the geographical range of d. reticulatus in europe is discontinuous with a gap separating eastern and western macroregions. new foci observed in several locations in western and central europe were considered an expansion of the western population, including foci in western poland. in the present paper we used molecular markers to identify the origins of the ...201728285892
black-backed jackals (canis mesomelas) are natural hosts of babesia rossi, the virulent causative agent of canine babesiosis in sub-saharan africa.babesia rossi, which is transmitted by haemaphysalis spp. and is highly virulent to domestic dogs, occurs only in sub-saharan africa. since dogs are not native to the region, it has been postulated that the natural host of b. rossi is an indigenous african canid. although various attempts at artificial infection indicated that black-backed jackals (canis mesomelas) could become subclinically infected with b. rossi, data on occurrence of b. rossi in free-ranging jackals was lacking. a long-term b ...201728285591
molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease.the spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (vbp), which could have a fundamental role in the pathogenesis of splenic disease. this study aimed to evaluate certain vbp in samples from dogs with splenic lesions.201728285583
utility of urinary markers in the assessment of renal dysfunction in canine babesiosis.canine babesiosis is a common and clinically significant tick-borne disease caused by haemoprotozoan parasites of the genus babesia. acute renal failure is considered to be one of the most prevalent complications of canine babesiosis. this complication leads to a decrease in the glomerular filtration rate and in consequence causes azotemia and uremia. the objective of this study was to assess the localization and extent of renal damage in dogs infected with babesia canis using an urinary marker ...201728074976
surveillance for viral and parasitic pathogens in a vulnerable african lion (panthera leo) population in the northern tuli game reserve, botswana.african lion ( panthera leo ) numbers are decreasing rapidly and populations are becoming smaller and more fragmented. infectious diseases are one of numerous issues threatening free-ranging lion populations, and low-density populations are particularly at risk. we collected data on the prevalence and diversity of viral and parasitic pathogens in a small lion population in eastern botswana. during 2012 and 2014, blood samples were collected from 59% (n=13) of the adult-subadult lions in the nort ...201727669009
dermacentor reticulatus (fabricius, 1794) and babesia canis (piana et galli-valerio, 1895) as the parasites of companion animals (dogs and cats) in the wrocław area, south-western poland.tests performed in 2013 and 2014 revealed the occurrence of three tick species parasitizing pet cats and dogs in the wrocław agglomeration. in total, 1,455 tick specimens were removed from 931 hosts (760 dogs and 171 cats) in 18 veterinary clinics. the dominant tick species was ixodes ricinus (n=1272; 87.4%), followed by i. hexagonus (n=137; 9.4%) and dermacentor reticulatus (n=46; 3.2%). females were the most often collected development stage among i. ricinus and d. reticulatus, and nymphs amon ...201727614478
prognostic markers in acute babesia canis infections.canine babesiosis, caused by babesia canis, is a prevalent and clinically relevant disease in europe. severe acute babesiosis is characterized by a high mortality but prognosis is not always correlated with clinical signs nor with the level of parasitemia.201726727465
relation of antioxidant status at admission and disease severity and outcome in dogs naturally infected with babesia canis canis.canine babesiosis is caused by species of the babesia genus and has become an emerging disease worldwide. to the authors' knowledge there are no reports in which antioxidants have been analyzed in different presentations of canine babesiosis or in which the prognostic value of antioxidants has been studied. the aim of this study was to evaluate whether oxidative stress could be related to the severity and outcome of canine babesiosis. for this purpose a profile consisting of four antioxidant bio ...201728438201
a new pcr assay for the detection and differentiation of babesia canis and babesia vogeli.babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. diagnosis of babesiosis is often impeded by the transient presence of the parasites in peripheral blood, as well as by their pleomorphic nature. given the reports of an expanding and, in some cases, sympatric geographical distribution of babesia canis and babesia vogeli in dogs and associated vectors, in europe, the development of time-effici ...201728739301
a pcr survey of vector-borne pathogens in different dog populations from turkey.in the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in konya district, turkey, and tested by specific pcr for the presence of vector-borne pathogens. several pathogens were identified, most of which can cause substantial morbidity in dogs. pcr results revealed that 54 (28.1%) dogs were infected with one or more pathogens. positive results were obtained for babesia spp. in 4 dogs (2.1%), hepatozoon spp. in 8 dogs (4.2%) and mycoplasma spp ...201728682765
a survey of canine haemoprotozoan parasites from turkey, including molecular evidence of an unnamed babesia.canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. a reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as hepatozoon canis. ten samples hybridized to the theileria/babesia and babesia catch all probes but did not hybridize to any species-specific probe tested, suggesting the presence of an unrec ...201728673460
first molecular detection of piroplasm infection in pet dogs from gansu, china.babesiosis, the hemolytic disease caused by babesia, which is a tick-transmitted obligate intraerythrocytic protozoan parasite. this disease is responsible for significant mortality and morbidity rates and enormous economic losses to the livestock industry in tropical and subtropical regions worldwide. in this study, blood samples were collected from 141 pet dogs from gansu, china, and analyzed for babesia or theileria spp. infection using specific pcr and sequencing based on 18s rrna gene fragm ...201728638376
molecular identification of babesia spp. in naturally infected dogs of kerala, south india.canine babesiosis is a tick borne haemoprotozoan disease caused by large and small intraerythrocytic apicomplexan piroplasms of the genus babesia spp. the clinical manifestations of the disease vary from inapparent subclinical form to hyperacute shock related haemolytic crisis. microscopic examination of blood smears from suspected dogs revealed babesia canis and b. gibsoni in 3.45 and 25.86 % of samples respectively. a seminested pcr based on previously published species specific primers target ...201728615859
prevalence and geographic distribution of vector-borne pathogens in apparently healthy dogs in croatia.vector-borne pathogens (vbps) are a group of globally extended and quickly spreading pathogens that are transmitted by various arthropod vectors. the aim of the present study was to investigate the seroprevalence against babesia canis, anaplasma phagocytophilum, borrelia burgdorferi sensu lato, leishmania infantum, dirofilaria immitis, and ehrlichia canis in dogs in croatia. we investigated 435 randomly selected apparently healthy dogs in 13 different locations of croatia for antibodies to b. ca ...201728448211
eurasian golden jackal as host of canine vector-borne protists.jackals are medium-sized canids from the wolf-like clade, exhibiting a unique combination of ancestral morphotypes, broad trophic niches, and close phylogenetic relationships with the wolf and dog. thus, they represent a potential host of several pathogens with diverse transmission routes. recently, populations of the eurasian golden jackal canis aureus have expanded into the western palaearctic, including most of europe. the aim of our study was to examine eurasian golden jackals from romania, ...201728410591
occurrence and diversity of arthropod-transmitted pathogens in red foxes (vulpes vulpes) in western austria, and possible vertical (transplacental) transmission of hepatozoon canis.red fox (vulpes vulpes) is the most abundant wild canid species in austria, and it is a well-known carrier of many pathogens of medical and veterinary concern. the main aim of the present study was to investigate the occurrence and diversity of protozoan, bacterial and filarial parasites transmitted by blood-feeding arthropods in a red fox population in western austria. blood (n = 351) and spleen (n = 506) samples from foxes were examined by pcr and sequencing and the following pathogens were id ...201728835291
new wide dynamic range assays for quantification of anti-leishmania igg2 and iga antibodies in canine serum.the aims of this study were (1) to develop and validate time resolved-immunofluorometric assays for the detection of anti-leishmania igg2 and iga antibodies in canine serum and (2) to evaluate the ability of these assays to quantify different amounts of anti-leishmania antibodies in leishmania-seronegative and seropositive dogs, determined by a commercial elisa assay, and between different clinical stages according to leishvet guidelines. the analytical validation showed that the assays had a go ...201728669382
prevalence and distribution of borrelia and babesia species in ticks feeding on dogs in the u.k.ticks were collected during march-july 2015 from dogs by veterinarians throughout the u.k. and used to estimate current prevalences and distributions of pathogens. dna was extracted from 4750 ticks and subjected to polymerase chain reaction and sequence analysis to identify borrelia burgdorferi sensu lato (spirochaetales: spirochaetaceae) and babesia (piroplasmida: babesiidae) species. of 4737 ticks [predominantly ixodes ricinus linneaus (ixodida: ixodidae)], b. burgdorferi s.l. was detected in ...201728846148
an elisa for the early diagnosis of acute canine babesiosis detecting circulating antigen of large babesia spp.babesia canis is the predominant babesia species in dogs in europe and is responsible for a severe and fatal disease. an increase in global pet tourism and a widening of the geographic distribution of the tick vector has led to the emergence of infections in areas where previously only imported cases have been reported. due to the potential for rapid and serious disease progression, direct parasite detection by stained blood smears and light microscopy or dna-based methods have traditionally bee ...201728807287
acute-phase response in babesia canis and dirofilaria immitis co-infections in dogs.babesia canis and dirofilaria immitis are emerging and geographically overlapping vector-borne pathogens in dogs. infection with b. canis leads to acute-phase response (apr) that can be mild to severe and results in either non-complicated or complicated forms of the disease. the aim of this study was to determine whether acute b. canis infection is more severe in dogs with underlying asymptomatic d. immitis infection. dogs of both sexes, different ages and breeds, with naturally occurring mono-i ...201728802807
high-resolution melting pcr assay, applicable for diagnostics and screening studies, allowing detection and differentiation of several babesia spp. infecting humans and animals.the goal of the study was to design a single tube pcr test for detection and differentiation of babesia species in dna samples obtained from diverse biological materials. a multiplex, single tube pcr test was designed for amplification of approximately 400 bp region of the babesia 18s rrna gene. universal primers were designed to match dna of multiple babesia spp. and to have low levels of similarity to dna sequences of other intracellular protozoa and babesia hosts. the pcr products amplified f ...201728795223
genome-wide analysis of gene expression and protein secretion of babesia canis during virulent infection identifies potential pathogenicity factors.infections of dogs with virulent strains of babesia canis are characterized by rapid onset and high mortality, comparable to complicated human malaria. as in other apicomplexan parasites, most babesia virulence factors responsible for survival and pathogenicity are secreted to the host cell surface and beyond where they remodel and biochemically modify the infected cell interacting with host proteins in a very specific manner. here, we investigated factors secreted by b. canis during acute infec ...201728611446
emergence of babesia canis in southern england.the united kingdom is considered free of autochthonous transmission of canine babesiosis although cases are reported in dogs associated with recent travel abroad. during the winter months of 2015/16, a cluster of cases of disease in dogs with signs suggestive of canine babesiosis were reported in harlow, essex.201728514953
a new leishmania-specific hypothetical protein and its non-described specific b cell conformational epitope applied in the serodiagnosis of canine visceral leishmaniasis.the serodiagnosis of canine visceral leishmaniasis (cvl) presents problems related to its sensitivity and/or specificity. in the present study, a new leishmania-specific hypothetical protein, lihyd, was produced as a recombinant protein (rlihyd) and evaluated in elisa experiments for the cvl serodiagnosis. lihyd was characterized as antigenic in a recent immunoproteomic search performed with leishmania infantum proteins and the sera of dogs developing visceral leishmaniasis (vl). aiming to compa ...201626782811
the southernmost foci of dermacentor reticulatus in italy and associated babesia canis infection in dogs.two clustered clinical cases of canine babesiosis were diagnosed by veterinary practitioners in two areas of northeastern italy close to natural parks. this study aimed to determine the seroprevalence of babesial infection in dogs, the etiological agents that cause canine babesiosis and the potential tick vector for the involved babesia spp.201627090579
babesia canis infection in ticks in essex. 201627013570
uneven seasonal distribution of babesia canis and its two 18s rdna genotypes in questing dermacentor reticulatus ticks in urban habitats.it has been reported from cities in central europe that clinical cases of canine babesiosis are most frequent in spring time, despite the fact that the peak activity of dermacentor reticulatus (the vector of babesia canis) is during autumn. the present study was initiated to evaluate the seasonal distribution of b. canis-infected d. reticulatus ticks in this context. in two habitats of budapest 852 d. reticulatus adults were collected between august, 2014 and june, 2015. among the molecularly an ...201627009915
babesia canis detected in dogs and associated ticks from essex. 201626940416
development of a quantitative pcr for the detection of rangelia vitalii.the aim of this study was to develop and validate a sybr green qpcr assay to detect and quantify a fragment of the 18s rrna gene of rangelia vitalii in canine blood. repeatability of the qpcr was determined by the intra- and inter-assay variations. the qpcr showed efficiency of e=101.30 (r(2)=0.996), detecting as few as one copy of plasmid containing the target dna. specificity of the assay was performed using dna samples of babesia canis, b. gibsoni, ehrlichia canis, e. ewingii and leishmania s ...201626827871
development of recombinant bgp12 based enzyme linked immunosorbent assays for serodiagnosis of babesia gibsoni infection in dogs.indirect elisa and dot-elisa using recombinant bgp12 (rbgp12) were developed for the diagnosis of babesia gibsoni infected dogs. the complete open reading frame of bgp12 gene (378bp) was cloned in pet-32a(+) expression vector and expressed in escherichia coli as a soluble thioredoxin (trx) fusion protein. the purified rbgp12 was used for production of anti-rbgp12 rabbit serum, which recognized a native 12-kda protein in b. gibsoni infected erythrocyte by western blot analysis. to evaluate the po ...201626827835
escalations in the incidence of canine babesiosis over a period of 8 years (2006-2013) in chennai, tamil nadu, india.epidemiological studies on the incidence and prevalence of disease will help in the monitoring and surveillance of diseases thus preventing occurrence of an epidemic. this study focuses on the variations in the incidences of canine babesiosis over a period of 8 years in chennai. variations have been noticed to in the incidence of the species of babesia affecting dogs. there has been an escalation in the incidence of b.gibsoni infection during the study period. the factors such as seasonal, age, ...201627876923
mitochondrial genome sequences and structures aid in the resolution of piroplasmida phylogeny.the taxonomy of the order piroplasmida, which includes a number of clinically and economically relevant organisms, is a hotly debated topic amongst parasitologists. three genera (babesia, theileria, and cytauxzoon) are recognized based on parasite life cycle characteristics, but molecular phylogenetic analyses of 18s sequences have suggested the presence of five or more distinct piroplasmida lineages. despite these important advancements, a few studies have been unable to define the taxonomic re ...201627832128
withdrawn:first report on babesia canis vogeli infection in dogs in the philippines.the publisher regrets that this article is an accidental duplication of an article that has already been published, http://dx.doi.org/10.1016/j.parint.2016.10.001.the duplicate article has therefore been withdrawn. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy.201627815229
confirmation of occurrence of <i>babesia vogeli</i> in a dog in windhoek, central namibia.although there is evidence of high seroprevalence of antibodies to babesia spp. in dogs in central namibia, clinical babesiosis is rarely diagnosed. rhipicephalus sanguineus sensu lato, the vector of babesia vogeli, is common in namibia while haemaphysalis elliptica, the vector of the highly virulent but morphologically indistinguishable babesia rossi, has rarely been recorded, mainly in northern namibia. on the basis of vector occurrence, clinical cases of canine babesiosis in windhoek, central ...201627796110
magnetic resonance imaging of brain involvement in dogs using paramagnetic contrast enhancement with mn(ii)-dcta p.experimental study of a new paramagnetic complex mn-dcta is carried out. the complex (0.5 m manganese(ii) solution with trans-1,2-diamine cyclohexane-n,n,n',n'-tetraacetic acid; cyclomang) was used for contrast magnetic resonance imaging visualization of cns involvement in dogs with severe forms of babesia canis infection. cns injuries were visualized in all cases, with highly intense contrasting at the expense of mn-dcta accumulation at the periphery of the damaged zone. quantitative evaluation ...201627704350
molecular detection of rickettsia conorii and other zoonotic spotted fever group rickettsiae in ticks, romania.the diverse tick fauna as well as the abundance of tick populations in romania represent potential risks for both human and animal health. spotted fever group (sfg) rickettsiae are recognized as important agents of emerging human tick-borne diseases worldwide. however, the epidemiology of rickettsial diseases has been poorly investigated in romania. in urban habitats, companion animals which are frequently exposed to tick infestation, play a role in maintenance of tick populations and as reservo ...201626507182
vector-borne pathogens in dogs and red foxes from the federal state of brandenburg, germany.dirofilaria repens is endemic in eastern and southern european regions but was recently found in germany in dogs, mosquitoes and one human patient. since some of the positive dog and mosquito samples were collected in brandenburg, it was aimed to systematically assess the prevalence of d. repens and other canine vector-borne pathogens in brandenburg. dog owners also received a questionnaire and were asked to provide more information about the dogs including travel history. in total, 1023 dog blo ...201627270389
prevalence of vector-borne pathogens in dogs from haiti.canine vector-borne pathogens are common on some caribbean islands, but survey data in haiti are lacking. to determine the prevalence of selected vector-borne pathogens in dogs from haiti, we tested blood samples collected from 210 owned dogs, 28 (13.3%) of which were infested with rhipicephalus sanguineus ticks at the time of blood collection. no other tick species were identified on these dogs. a commercially available elisa identified antibodies to ehrlichia spp. in 69 (32.9%), antibodies to ...201627270383
molecular detection of emerging tick-borne pathogens in vojvodina, serbia.ticks play an important role in disease transmission globally due to their capability to serve as vectors for human and animal pathogens. the republic of serbia is an endemic area for a large number of tick-borne diseases. however, current knowledge on these diseases in serbia is limited. the aim of this study was to investigate the presence of new emerging tick-borne pathogens in ticks collected from dogs and the vegetation from different parts of vojvodina, serbia. a total of 187 ticks, includ ...201626565929
first molecular identification of babesia gibsoni in dogs from slovakia, central europe.canine babesiosis is a severe and potentially life threatening infection. in europe, babesia canis is considered to be the most common species responsible for the disease. we report two cases of babesiosis caused by babesia gibsoni. the polymerase chain reaction, restriction fragment length polymorphism analysis and further sequencing of 18s rrna gene fragments from blood samples of both dogs revealed the identity of isolates with b. gibsoni genotypes from other dogs worldwide. this species was ...201626304014
exposure to vector-borne pathogens in candidate blood donor and free-roaming dogs of northeast italy.many vector-borne pathogens including viruses, bacteria, protozoa and nematodes occur in northeast italy, representing a potential threat to animal and human populations. little information is available on the circulation of the above vector-borne pathogens in dogs. this work aims to (i) assess exposure to and circulation of pathogens transmitted to dogs in northeast italy by ticks, sandflies, and mosquitoes, and (ii) drive blood donor screening at the newly established canine blood bank of the ...201627357128
immunopathological features of canine myocarditis associated with leishmania infantum infection.myocarditis associated with infectious diseases may occur in dogs, including those caused by the protozoa neospora caninum, trypanosoma cruzi, babesia canis, and hepatozoon canis. however, although cardiac disease due to leishmania infection has also been documented, the immunopathological features of myocarditis have not been reported so far. the aim of this study was to examine the types of cellular infiltrates and expression of mhc classes i and ii in myocardial samples obtained at necropsy f ...201627413751
dermacentor reticulatus: a vector on the rise.dermacentor reticulatus is a hard tick species with extraordinary biological features. it has a high reproduction rate, a rapid developmental cycle, and is also able to overcome years of unfavourable conditions. dermacentor reticulatus can survive under water for several months and is cold-hardy even compared to other tick species. it has a wide host range: over 60 different wild and domesticated hosts are known for the three active developmental stages. its high adaptiveness gives an edge to th ...201627251148
babesia spp. in ticks and wildlife in different habitat types of slovakia.babesiosis is an emerging and potentially zoonotic disease caused by tick-borne piroplasmids of the babesia genus. new genetic variants of piroplasmids with unknown associations to vectors and hosts are recognized. data on the occurrence of babesia spp. in ticks and wildlife widen the knowledge on the geographical distribution and circulation of piroplasmids in natural foci. questing and rodent-attached ticks, rodents, and birds were screened for the presence of babesia-specific dna using molecu ...201627207099
fatal babesia canis canis infection in a splenectomized estonian dog.a previously splenectomized dog from estonia was presented with a sudden lack of appetite and discoloration of the urine. despite supportive therapy, its condition deteriorated dramatically during 1 day. severe thrombocytopenia and high numbers of protozoan hemoparasites were evident in blood smears, and the hematocrit dropped from 46 to 33 %. the dog was euthanized before specific antibabesial treatment was initiated. blood samples from the dog and from two other dogs in the same household test ...201626810086
canine babesiosis and tick activity monitored using companion animal electronic health records in the uk.recent publications highlighting autochthonous babesia canis infection in dogs from essex that have not travelled outside the uk are a powerful reminder of the potential for pathogen emergence in new populations. here the authors use electronic health data collected from two diagnostic laboratories and a network of 392 veterinary premises to describe canine babesia cases and levels of babesia concern from january 2015 to march 2016, and the activity of ticks during december 2015-march 2016. in m ...201627484328
the prevalence of dirofilaria repens in cats, healthy dogs and dogs with concurrent babesiosis in an expansion zone in central europe.dirofilaria repens is a mosquito-transmitted, filarial nematode parasitizing dogs, cats and other carnivores. recently, this parasite has spread in central europe, including poland. the aim of the present study was to estimate the prevalence of d. repens in cats and dogs in different regions of the country and to investigate the occurrence and consequences of co-infection with another fast-spreading vector-borne parasite, babesia canis.201627595920
prevention of transmission of babesia canis by dermacentor reticulatus ticks to dogs after topical administration of fluralaner spot-on solution.the preventive effect of fluralaner spot-on solution against transmission of babesia canis by dermacentor reticulatus ticks was evaluated.201627241120
molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of babesia canis (bcmsa1 and bcsa1).babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in europe and west asia. despite its importance, there is no known rapid diagnostic kit detection of b. canis infection in dogs. the present study identified two novel antigens of b. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of b. canis infection.201627141812
screening of bat faeces for arthropod-borne apicomplexan protozoa: babesia canis and besnoitia besnoiti-like sequences from chiroptera.bats are among the most eco-epidemiologically important mammals, owing to their presence in human settlements and animal keeping facilities. roosting of bats in buildings may bring pathogens of veterinary-medical importance into the environment of domestic animals and humans. in this context bats have long been studied as carriers of various pathogen groups. however, despite their close association with arthropods (both in their food and as their ectoparasites), only a few molecular surveys have ...201526315069
novel foci of dermacentor reticulatus ticks infected with babesia canis and babesia caballi in the netherlands and in belgium.autochthonous populations of dermacentor reticulatus ticks in the netherlands were discovered after fatal cases of babesiosis occurred in resident dogs in 2004. the presence of d. reticulatus in the netherlands has also linked with the emergence of piroplasmosis in the resident horse population. the aim of this study was to put together results of continued surveillance of field sites and hosts for this tick in the netherlands and also in belgium and determine their infection status for babesia ...201525889392
prevention of babesia canis in dogs: efficacy of a fixed combination of permethrin and fipronil (effitix®) using an experimental transmission blocking model with infected dermacentor reticulatus ticks.two experimental studies using a transmission blocking model with dermacentor reticulatus ticks infected with babesia canis were performed to test the ability of effitix® to prevent the transmission of babesiosis in dogs.201525595325
update on epidemiology of canine babesiosis in southern france.canine babesiosis is an emerging or re-emerging disease caused by babesia and theileria protozoans, also called piroplasms, transmitted by ixodid ticks. in europe, four etiological agents have been identified to date, namely babesia canis, b. vogeli, b. gibsoni and theileria annae. france has a high prevalence of canine babesiosis and two tick species, dermacentor reticulatus and rhipicephalus sanguineus, are supposed to transmit b. canis and b. vogeli respectively. in southern france, where dog ...201526303260
first report on babesia cf. microti infection of red foxes (vulpes vulpes) from hungary.to date, only one report of a small babesia infection based on microscopic observation which caused babesiosis in two dogs in hungary has been published. babesiosis due to babesia canis - which is endemic in the local dogs - has only been detected in captive grey wolves. no information is available on babesial/theilerial infections in red foxes in hungary. the aim of the study was to screen red foxes in hungary for babesial parasites by pcr and to compare their partial 18s rrna gene sequences to ...201525623386
repellency, prevention of attachment and acaricidal efficacy of a new combination of fipronil and permethrin against the main vector of canine babesiosis in europe, dermacentor reticulatus ticks.dermacentor reticulatus is a european hard tick of major veterinary importance because it is the vector of canine babesiosis due to babesia canis. the efficacy against this particular tick species is therefore a key characteristic for an acaricidal solution for dogs. the repellency, prevention of attachment and acaricidal efficacy of frontline tri- act/frontect, a new combination of fipronil and permethrin against induced infestations of dermacentor reticulatus ticks on dogs were evaluated after ...201525622802
a novel combination of fipronil and permethrin (frontline tri-act®/frontect®) reduces risk of transmission of babesia canis by dermacentor reticulatus and of ehrlichia canis by rhipicephalus sanguineus ticks to dogs.the ability of frontline tri-act®/frontect®, a topical ectoparasiticide containing fipronil and permethrin for dogs, to prevent the transmission of babesia canis as well as ehrlichia canis was evaluated by infesting dogs with infected vector ticks.201526586365
prevention of transmission of babesia canis by dermacentor reticulatus ticks to dogs treated orally with fluralaner chewable tablets (bravecto™).the preventive effect of fluralaner chewable tablets (bravecto™) against transmission of babesia canis by dermacentor reticulatus ticks was evaluated.201526040319
molecular detection of tick-borne rickettsial and protozoan pathogens in domestic dogs from turkey.canine tick-borne parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. in addition to their veterinary importance, domestic dogs play an important role in the transmission cycles of some agents by acting as reservoirs and sentinels. this study investigated babesia, theileria, anaplasma, and ehrlichia species in asymptomatic dogs in ten provinces of turkey.201525889911
pathogens vectored by the tick, dermacentor reticulatus, in endemic regions and zones of expansion in poland.dermacentor reticulatus plays an important role in the maintenance of pathogens of medical and veterinary importance in the environment. currently two isolated populations of d. reticulatus are present in poland--western and eastern. the range of the eastern population covers endemic areas in eastern poland but this population is expanding westwards creating an expansion zone in the centre of the country. the expansion zone in western poland is occupied by the recently discovered western populat ...201526403456
a leishmania-specific hypothetical protein expressed in both promastigote and amastigote stages of leishmania infantum employed for the serodiagnosis of, and as a vaccine candidate against, visceral leishmaniasis.lihyv is an antigenic hypothetical protein present in both promastigote and amastigote stages of leishmania infantum, which was recently identified by an immunoproteomic approach. a recombinant version of this protein (rlihyv) was evaluated as a diagnostic marker for canine vl (cvl). in addition, the prophylactic efficacy of the rlihyv protein, and two of its cd8(+) t cell epitopes, has been analyzed in a murine model of visceral leishmaniasis (vl).201526160291
classification of babesia canis strains in europe based on polymorphism of the bc28.1-gene from the babesia canis bc28 multigene family.the vast majority of clinical babesiosis cases in dogs in europe is caused by babesia canis. although dogs can be vaccinated, the level of protection is highly variable, which might be due to genetic diversity of b. canis strains. one of the major merozoite surface antigens of b. canis is a protein with a mr of 28 kda that belongs to the bc28 multigene family, that comprises at least two genes, bc28.1 and a homologous bc28.2 gene. the two genes are relatively conserved but they are very distinct ...201526092188
comparative profiling of micrornas in male and female rhipicephalus sanguineus.rhipicephalus sanguineus is an ectoparasite of medical and veterinary significance, which can transmit a number of pathogens including babesia canis, ehrlichia canis, and rickettsia conorii. micrornas (mirnas) are recognized as regulators in sex differentiation in dioecious species. we here investigated and compared the mirna profiles of male and female adults of r. sanguineus by combining solexa deep sequencing with bioinformatics platform and quantitative real-time pcr. a total of 11.88 and 16 ...201526054615
clinical babesiosis and molecular identification of babesia canis and babesia gibsoni infections in dogs from serbia.canine babesiosis is a frequent and clinically significant tick-borne disease. sixty symptomatic dogs with clinical findings compatible with babesiosis were included in this study conducted in serbia. after clinical examination, blood samples were taken for microscopic examination, complete blood count (cbc), canine snap 4dx test, dna analyses and sequencing. the main clinical signs included apathy, anorexia, fever, brown/red discoloration of urine, pale mucous membranes, icterus, splenomegaly, ...201526051258
low t3 syndrome in canine babesiosis associated with increased serum il-6 concentration and azotaemia.low triiodothyronine (t3) syndrome, also named euthyroid sick syndrome or non-thyroidal illness syndrome, has been recognized in canine babesiosis caused by babesia rossi, where it manifested by lowering of the serum thyrotropin (tsh), total thyroxin (tt4) and free thyroxin (ft4) concentrations. this syndrome has also been observed in critical diseases in humans and animals, and the severity of the disease is considered an important factor in lowering of thyroid hormone concentrations. interleuk ...201525976636
leishmania, babesia and ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?the present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-leishmania spp., -babesia canis vogeli and -ehrlichia canis antibodies in urban dogs from an area endemic to these parasites.201525860466
detection of babesia canis vogeli and hepatozoon canis in canine blood by a single-tube real-time fluorescence resonance energy transfer polymerase chain reaction assay and melting curve analysis.a real-time fluorescence resonance energy transfer polymerase chain reaction (qfret pcr) coupled with melting curve analysis was developed for detection of babesia canis vogeli and hepatozoon canis infections in canine blood samples in a single tube assay. the target of the assay was a region within the 18s ribosomal rna gene amplified in either species by a single pair of primers. following amplification from the dna of infected dog blood, a fluorescence melting curve analysis was done. the 2 s ...201525776544
changes in selected subpopulations of lymphocytes in dogs infected with babesia canis treated with imidocarb.the purpose of this study was to track changes in selected subpopulations of lymphocytes in the blood of dogs infected with babesia (b.) canis and treated with imidocarb.201525771779
development of loop-mediated isothermal amplification (lamp) for detection of babesia gibsoni infection in dogs.diagnosis of canine babesiosis, caused by babesia gibsoni is difficult, especially in chronically infected dogs. a loop mediated isothermal amplification (lamp) assay was developed and standardized by using four oligonucleotide primers targeting the hypervariable region of 18s rrna gene (genbank acc. no. kc461261). the primers specifically amplified b. gibsoni dna, while no amplification was detected with dna from non-infected dogs as well as from dogs infected with babesia canis vogeli, hepatoz ...201525749021
distribution patterns of babesia gibsoni infection in hunting dogs from nine japanese islands.canine babesiosis constitutes a major global veterinary medical problem caused by tick-borne hemoparasites babesia gibsoni and babesia canis. babesia gibsoni induces more severe clinical signs and is mainly transmitted by the ixodid haemaphysalis longicornis. in japan, b. gibsoni is primarily found in the western districts, with few records in the eastern parts. the aim of the current investigation was to evaluate distribution patterns of b. gibsoni infection in 9 japanese islands and peninsulas ...201525419881
hyperaldosteronism and its association with hypotension and azotaemia in canine babesiosis.the previous work on canine babesiosis revealed hypokalaemia and increased fractional excretion of potassium in infected dogs suggesting a role for aldosterone in the loss of potassium via the kidneys in affected dogs. moreover, hypotension, which is one of the complications of canine babesiosis leading to renal failure, may induce secondary hyperaldosteronism.201525347616
current surveys of the seroprevalence of borrelia burgdorferi, ehrlichia canis, anaplasma phagocytophilum, leishmania infantum, babesia canis, angiostrongylus vasorum and dirofilaria immitis in dogs in bulgaria.canine vector-borne diseases (cvbds) have increasingly become a focus of interest in recent years. some of the cvbds are zoonotic and may therefore also represent a risk for the human population. different factors are in discussion to explain the expansion of vectors and pathogens into formerly unaffected areas. knowledge of the prevalence and distribution of cvbds in bulgaria is scant overall and most data rely on single case descriptions. the aim of the present study was to determine the serop ...201526152413
tick-borne diseases (borreliosis, anaplasmosis, babesiosis) in german and austrian dogs: status quo and review of distribution, transmission, clinical findings, diagnostics and prophylaxis.tick-borne diseases (tbd) in dogs have gained in significance in german and austrian veterinary practices. the widespread european tick species ixodes ricinus represents an important vector for spirochaetes of the borrelia burgdorferi sensu lato group and rickettsiales such as anaplasma phagocytophilum. the meadow or ornate dog tick (dermacentor reticulatus) is an important vector for babesia canis, as is the brown dog tick (rhipicephalus sanguineus) for babesia vogeli in the mediterranean regio ...201526152408
vector-borne diseases of small companion animals in namibia: literature review, knowledge gaps and opportunity for a one health approach.namibia has a rich history in veterinary health but little is known about the vector-borne diseases that affect companion dogs and cats. the aim of this review is to summarise the existing published and available unpublished literature, put it into a wider geographical context, and explore some significant knowledge gaps. to date, only two filarial pathogens (dirofilaria repens and acanthocheilonema dracunculoides) and three tick-borne pathogens (babesia canis vogeli, hepatozoon canis and ehrlic ...201526824340
identification of new 18s rrna strains of babesia canis isolated from dogs with subclinical babesiosis.in this study, we used pcr to detect and characterize b. canis from naturally infected dogs in poland with subclinical babesiosis by amplifying and sequencing a portion of the 18s ribosomal rna (rrna) gene. venous blood samples were collected from ten dogs with subclinical babesiosis. a 559-bp fragment of the b. canis 18s rrna gene was amplified by pcr. sequencing of the pcr products led to the identification of a new variant of babesia canis, differing from the previously detected protozoa geno ...201526618590
molecular detection of ehrlichia canis, hepatozoon canis and babesia canis vogeli in stray dogs in mahasarakham province, thailand.canine tick borne diseases showing distribution worldwide have caused morbidity and mortality in dogs. this study observed the mainly tick borne pathogens described for dogs in thailand, ehrlichia canis, hepatozoon canis and babesia canis vogeli. from may to july 2014, blood samples were collected from 79 stray dogs from 7 districts of mahasarakham province to molecular surveyed for 16s rrna gene of e. canis and 18s rrna gene of h. canis and b. canis vogeli. twenty eight (35.44%) of stray dogs s ...201526568991
human exposure to piroplasms in central and northern italy.ta serosurvey has been conducted in northern and central italy to investigate the presence in humans of antibodies against zoonotic babesia and theileria species. the study focused on a total of 432 volunteers, of which 290 were persistently exposed to tick bites because of their jobs (forester employees, livestock keepers, veterinary practitioners, farmers and hunters) and 142 resident in the same area less frequently exposed. an indirect fluorescent antibody test (ifat) for humans was used to ...201424715592
the infection of questing dermacentor reticulatus ticks with babesia canis and anaplasma phagocytophilum in the chernobyl exclusion zone.tick occurrence was studied in the chernobyl exclusion zone (cez) during the august-october 2009-2012. dermacentor reticulatus ticks were collected using the flagging method and then screened for infection with anaplasma phagocytophilum and babesia canis by a pcr method incorporating specific primers and sequence analysis. the prevalence of infection with b. canis canis and a. phagocytophilum was found to be 3.41% and 25.36%, respectively. the results present the first evidence of b. canis canis ...201424953751
molecular detection of co-infections with anaplasma phagocytophilum and/or babesia canis canis in dirofilaria-positive dogs from slovakia.recently, several arthropod-borne infections have been introduced into previously non-endemic regions in europe as the result of various global changes. at the same time, endemic regions are expanding and the risk of co-infections is rising, due to climate change that allows vectors to move and spread infectious diseases into new areas. the aim of the current study was to confirm simultaneous infections with anaplasma phagocytophilum and/or babesia canis canis in dirofilaria-infected dogs from s ...201424630708
the risk of vector-borne infections in sled dogs associated with existing and new endemic areas in poland: part 1: a population study on sled dogs during the racing season.the achievements of sled dogs in competitions depend both on their training and on their health. vector-borne infections may lead to anaemia, affect joints or heart muscle or even cause death. between december 2009 and october 2010, one hundred and twenty six individual blood samples were collected from 26 sled dog kennels situated in different regions of poland. the majority of samples were taken during the racing season (winter 2009/10). the prevalences of 3 vector-borne infections- including ...201424491396
regional seroreactivity and vector-borne disease co-exposures in dogs in the united states from 2004-2010: utility of canine surveillance.vector-borne disease (vbd) pathogens remain an emerging health concern for animals and humans throughout the world. surveillance studies of ticks and humans have made substantial contributions to our knowledge of vbd epidemiology trends, but long-term vbd surveillance data of dogs in the united states is limited. this seroreactivity study assessed us temporal and regional trends and co-exposures to anaplasma, babesia, bartonella, borrelia burgdorferi, dirofilaria immitis, ehrlichia spp., and spo ...201425325316
the first evidence for vertical transmission of babesia canis in a litter of central asian shepherd dogs.tick-borne infections constitute an increasing health problem in dogs and may lead to death, especially in young or elderly individuals. canine babesiosis constitutes a serious health problem in dogs worldwide. the aim of the study was to verify the probability of vertical transmission of babesia canis between the bitch and the pups.201425292117
detection and molecular characterization of canine babesiosis causative agent babesia canis in the naturally infected dog in lithuania.canine babesiosis caused by babesia canis is an emerging infectious disease in europe. although previously uncommon, canine babesiosis has become quite frequent in lithuania during the past decade. in the last few years an increasing number of cases with a wide variety of clinical signs have been recorded throughout the country. in lithuania the identification of the disease agent in veterinarian clinics is based on a microscopic analysis of size and morphology. to date, no data on the genetic c ...201425257504
development and evaluation of serodiagnostic assays with recombinant bgsa1 of babesia gibsoni.indirect elisa, dot-elisa and double antibody sandwich elisa (das-elisa) using truncated recombinant bgsa1 (rbgsa1) were developed for detecting babesia gibsoni infection in naturally infected dogs. truncated bgsa1 gene of 858 bp, encoding 32 kda protein was cloned in pet-32a(+) expression vector, expressed in escherichia coli and the recombinant protein was purified under native conditions. to evaluate the ability of the truncated rbgsa1as serodiagnostic reagent for b. gibsoni infection, a pane ...201425241636
application the mass spectrometry maldi-tof technique for detection of babesia canis canis infection in dogs.the aim of this study was to use rapid mass spectrometry (ms)-based proteomics analyses for diagnosis of babesia canis canis infections in dogs. the study was conducted on two groups of dogs--healthy dogs and dogs infected with b. canis canis which demonstrated symptoms of babesiosis. the matrix-assisted laser desorption ionization time-of-flight (maldi-tof) ms technique revealed the presence of a protein fraction of 51-52 kda in the blood serum of all the animals infected with the protozoa, whi ...201425238794
genetic characterization and phylogenetic relationships based on 18s rrna and its1 region of small form of canine babesia spp. from india.canine babesiosis is a vector borne disease caused by intra-erythrocytic apicomplexan parasites babesia canis (large form) and babesia gibsoni (small form), throughout the globe. apart from few sporadic reports on the occurrence of b. gibsoni infection in dogs, no attempt has been made to characterize babesia spp. of dogs in india. fifteen canine blood samples, positive for small form of babesia, collected from northern to eastern parts of india, were used for amplification of 18s rrna gene (∼16 ...201425120099
increased serum urea to creatinine ratio and its negative correlation with arterial pressure in canine babesiosis.the increase of the serum urea to creatinine ratio (ucr) was observed in dogs infected with babesia canis. previous studies have suggested that decrease of blood pressure can be one of the reasons for this phenomenon. in this work statistically significant increase of the ucr was observed in dogs with babesiosis. comparison of the ucr between 23 azotaemic dogs and 25 non-azotaemic dogs infected with babesia canis showed statistically significantly higher mean of the ucr in azotaemic dogs. correl ...201425119372
sympatric occurrence of ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks and rickettsia and babesia species in slovakia.vojka nad dunajom in the south-west of the slovak republic is a locality with sympatric occurrence of 3 species of ticks. this study investigated the spatial distribution of dermacentor reticulatus, ixodes ricinus, and haemaphysalis concinna ticks in this area and determined the prevalence of babesia and rickettsia species in questing adults of these tick species considered as potential risk for humans and animals. ticks were collected by blanket dragging over the vegetation from september 2011 ...201424973275
first report of rangelia vitalii infection (canine rangeliosis) in argentina.a 12-year old mixed breed neutered bitch from misiones, argentina, was presented with a history of fever and epistaxis. blood, bone marrow, and lymph node samples were collected for hematology and cytology. mild regenerative anemia was recorded and large, round, poorly stained piroplasms (>2.5 μm) were found within erythrocytes in blood and lymph node smears. nested pcr-rflp on blood and bone marrow samples was positive for piroplasm dna. the 18s rrna gene of piroplasms was targeted. a restricti ...201424970768
identification of serum biomarkers in dogs naturally infected with babesia canis canis using a proteomic approach.canine babesiosis is a tick-borne disease that is caused by the haemoprotozoan parasites of the genus babesia. there are limited data on serum proteomics in dogs, and none of the effect of babesiosis on the serum proteome. the aim of this study was to identify the potential serum biomarkers of babesiosis using proteomic techniques in order to increase our understanding about disease pathogenesis.201424885808
[detection of babesia canis (piroplasmida) dna in the blood samples and lysates of the ticks dermacentor reticulatus (ixodidae) collected in the tula and moscow regions].chimeric primers, the sensitivity and specificity of which allow them to be used in both the clinical setting and the epizootological assessment of tick infection by a real-time polymerase chain reaction (rt-pcr) assay, have been designed against babesia canis infection. the findings suggest that a large number of babesia dna copies are detectable in the blood in acute babesiosis. some animals that had experienced babesiosis developed blood b. canis carriage--a small number oftrophozoites remain ...201424738223
optimization of a molecular method for the diagnosis of canine babesiosis.babesiosis is a hemolytic disease caused by protozoans of the genus babesia (apicomplexa). this disease occurs worldwide and is transmitted by ticks to a variety of mammals, including humans. the objective of the present study was to optimize a molecular approach for the detection of a fragment of 18s rdna of babesia canis, babesia vogeli, babesia rossi or babesia gibsoni based on a single semi-nested polymerase chain reaction (pcr), and compare the efficiency of this approach with that of a sim ...201424728371
association between decreased blood pressure and azotaemia in canine babesiosis.acute tubular necrosis (atn) was described in canine babesiosis. hypotension is considered as one of the factors which influence the development of hypoxic renal damage. in this study hypotension defined as mean arterial pressure (map) < 80 mmhg was detected in 7 out of 48 dogs (14.6%) infected with babesia canis. lower systolic arterial pressure (sap), diastolic arterial pressure (dap) and map were detected in azotaemic dogs infected with b. canis. statistically significant negative correlation ...201424724487
the risk of vector-borne infections in sled dogs associated with existing and new endemic areas in poland. part 2: occurrence and control of babesiosis in a sled dog kennel during a 13-year-long period.the achievements of sled dogs in competitions depend both on their training and on their health. vector-borne infections may lead to anemia, affect joints or heart muscles or even cause death. canine babesiosis is an emerging, quickly spreading tick-borne disease in central europe. over a 13-year period (2000-2012) the occurrence of babesiosis cases was analyzed in one sled dog kennel situated in kury, a village near tłuszcz (n 52°24'56.78″, e 21°30'37.55″) in central poland. twenty cases/episod ...201424612743
serological cross-reactivity of trypanosoma cruzi, ehrlichia canis, toxoplasma gondii, neospora caninum and babesia canis to leishmania infantum chagasi tests in dogs.the aim of this study was to evaluate the serological cross-reactivity between leishmania sp. and other canine pathogens.201424603745
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