Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| first description of leishmania (viannia) infection in evandromyia saulensis, pressatia sp. and trichophoromyia auraensis (psychodidae: phlebotominae) in a transmission area of cutaneous leishmaniasis in acre state, amazon basin, brazil. | studies on the sandfly fauna to evaluate natural infection indexes are still limited in the brazilian amazon, a region with an increasing incidence of cutaneous leishmaniasis. here, by using a multiplex polymerase chain reaction directed to leishmania kdna and hybridisation, we were able to identify l. (viannia) subgenus in 12 out of 173 sandflies captured in the municipality of rio branco, acre state, revealing a positivity of 6.94%. by sequencing the leishmania 234 bp-hsp70 amplified products ... | 2017 | 28076470 |
| safety evaluation of a vaccine: effect in maternal reproductive outcome and fetal anomaly frequency in rats using a leishmanial vaccine as a model. | while the immunogenic potential of the vaccination against infectious diseases was extensively shown, data on the safety assessment of recombinant proteins in vaccine formulations administered during pregnancy are still scarce. in the current study, the antigenicity of a vaccine against leishmaniasis (based on leishmania braziliensis recombinant protein peroxidoxin) during pregnancy and possible maternal reproductive outcomes and fetal anomalies after immunization with a leishmanial vaccine or a ... | 2017 | 28249007 |
| performance of leishmania braziliensis enolase protein for the serodiagnosis of canine and human visceral leishmaniosis. | in the present study, leishmania braziliensis enolase was cloned and the recombinant protein (renolase) was evaluated for the serodiagnosis of canine and human visceral leishmaniosis (vl). for the canine vl diagnosis, this study examined serum samples of leishmania infantum-infected dogs, from non-infected animals living in endemic or non-endemic areas of leishmaniosis, as well as those from leish-tec(®)-vaccinated dogs and trypanosoma cruzi or ehrlichia canis experimentally infected animals. fo ... | 2017 | 28385540 |
| cutaneous leishmaniasis in french guiana: revising epidemiology with pcr-rflp. | the development of polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in french guiana. | 2017 | 28265182 |
| cytokines and microbicidal molecules regulated by il-32 in thp-1-derived human macrophages infected with new world leishmania species. | interleukin-32 (il-32) is expressed in lesions of patients with american tegumentary leishmaniasis (atl), but its precise role in the disease remains unknown. | 2017 | 28241012 |
| in vitro antiprotozoal activity of (s)-cis-verbenol against leishmania spp. and trypanosoma cruzi. | (s)-cis-verbenol, a monoterpene frequently found as a component of essential oils, was assayed against leishmania amazonensis, leishmania infantum, leishmania brasiliensis and against two strains of trypanosoma cruzi. the cytotoxicity of the compound was also assayed against human fibroblast cells using a colorimetric method. benznidazole was used as reference drug against t. cruzi and amphotericin b was used against leishmania spp. the compound showed good activity against the trypanosomes, bei ... | 2017 | 28062234 |
| use of recombinant antigens for sensitive serodiagnosis of american tegumentary leishmaniasis caused by different leishmania species. | american tegumentary leishmaniasis (atl) (also known as cutaneous leishmaniasis [cl]) is caused by various species of protozoa of the genus leishmania the diagnosis is achieved on a clinical, epidemiological, and pathological basis, supported by positive parasitological exams and demonstration of leishmanin delayed-type hypersensitivity. serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particular leishmania species causing the ... | 2017 | 27927927 |
| a vaccine composed of a hypothetical protein and the eukaryotic initiation factor 5a from leishmania braziliensis cross-protection against leishmania amazonensis infection. | in the present study, two proteins cloned from leishmania braziliensis species, a hypothetical protein (lbhyp) and the eukaryotic initiation factor 5a (eif5a), were evaluated to protect balb/c mice against l. amazonensis infection. the animals were immunized with the antigens, either separately or in combination, using saponin as an immune adjuvant in both cases. spleen cells from vaccinated and later infected mice produced significantly higher levels of protein and parasite-specific ifn-γ, il-1 ... | 2017 | 27693018 |
| a proteomic road to acquire an accurate serological diagnosis for human tegumentary leishmaniasis. | diagnostic tools are important for clinical management and epidemiological evaluation of tegumentary (tl) and visceral (vl) leishmaniasis. serology is not frequently used for the diagnosis of the tl form because low antibody titers and cross-reaction with vl. therefore, it is crucial to identify specific and immunogenic antigens from species associated with the tl form. here we employed a proteomic approach coupled to an in silico analysis and identified the most abundant and immunogenic protein ... | 2017 | 27262223 |
| antileishmanial activity of a calophyllum brasiliense leaf extract. | calophyllum brasiliense (clusiaceae) is a tree that grows mainly in the atlantic forest in brazil. its leaves possess antibacterial activity, cytotoxic activity against certain tumor cell lines, and antimicrobial activity in balb/c mice infected with leishmania (leishmania) amazonensis.aiming to identify ultrastructural changes and dna fragmentation in leishmania (viannia) braziliensis, promastigotes were treated with a concentration of the dichloromethane extract and coumarin (-) mammea a/bb fr ... | 2017 | 27224267 |
| new primers for detection of leishmania infantum using polymerase chain reaction. | leishmania infantum causes visceral leishmaniasis (vl) in the new world. the diagnosis of vl is confirmed by parasitological and serological tests, which are not always sensitive or specific. our aim was to design new primers to perform a polymerase chain reaction (pcr) for detecting l. infantum. sequences of the minicircle kinetoplast dna (kdna) were obtained from genbank, and the flc2/rlc2 primers were designed. samples of dna from l. infantum, leishmania amazonensis, leishmania braziliensis, ... | 2017 | 26603223 |
| simple dialkyl pyrazole-3,5-dicarboxylates show in vitro and in vivo activity against disease-causing trypanosomatids. | the synthesis and antiprotozoal activity of some simple dialkyl pyrazole-3,5-dicarboxylates (compounds 2-6) and their sodium salts (pyrazolates) (compounds 7-9) against trypanosoma cruzi, leishmania infantum and leishmania braziliensis are reported. in most cases the studied compounds showed, especially against the clinically significant amastigote forms, in vitro activities higher than those of the reference drugs (benznidazole for t. cruzi and glucantime for leishmania spp.); furthermore, the ... | 2017 | 28367781 |
| synthetic peptides as potential antigens for cutaneous leishmaniosis diagnosis. | this work's goal was to research new candidate antigens for cutaneous leishmaniosis (cl). in order to reach the goal, we used random peptide phage display libraries screened using antibodies from leishmania braziliensis patients. after selection, three peptides (p1, p2, and p3) were synthesized using fmoc chemistry. the peptides individually or a mixture of them (mix) was subsequently emulsified in complete and incomplete freund's adjuvant and injected subcutaneously in golden hamsters. sera fro ... | 2017 | 28367456 |
| species-specific ecological niche modelling predicts different range contractions for lutzomyia intermedia and a related vector of leishmania braziliensis following climate change in south america. | before 1996 the phlebotomine sand fly lutzomyia neivai was usually treated as a synonym of the morphologically similar lutzomyia intermedia, which has long been considered a vector of leishmania braziliensis, the causative agent of much cutaneous leishmaniasis in south america. this report investigates the likely range changes of both sand fly species in response to a stabilisation climate change scenario (rcp4.5) and a high greenhouse gas emissions one (rcp8.5). | 2017 | 28340594 |
| in vitro and in vivo antileishmanial activity of artemisia annua l. leaf powder and its potential usefulness in the treatment of uncomplicated cutaneous leishmaniasis in humans. | cutaneous leishmaniasis (cl) is a tropical disease that affects millions of individuals worldwide. the current drugs for cl may be effective but have serious side effects; hence, alternatives are urgently needed. although plant-derived materials are used for the treatment of various diseases in 80% of the global population, the validation of these products is essential. gelatin capsules containing dried artemisia annua leaf powder were recently developed as a new herbal formulation (totum) for t ... | 2017 | 28327802 |
| seasonality of sand flies (diptera: psychodidae) and leishmania dna detection in vector species in an area with endemic visceral leishmaniasis. | leishmaniases are a serious health problem in southeast brazil, including the city of belo horizonte (bh), minas gerais state (mg), where there are high rates of incidence and mortality due to visceral leishmaniases. bh is divided into nine sanitary districts (sd) of which one, the venda nova sd, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. | 2017 | 28327794 |
| a vaccine therapy for canine visceral leishmaniasis promoted significant improvement of clinical and immune status with reduction in parasite burden. | herein, we evaluated the treatment strategy employing a therapeutic heterologous vaccine composed of antigens of leishmania braziliensis associated with mpl adjuvant (lbmpl vaccine) for visceral leishmaniasis (vl) in symptomatic dogs naturally infected by leishmania infantum. sixteen dogs received immunotherapy with mpl adjuvant (n = 6) or with a vaccine composed of antigens of l. braziliensis associated with mpl (lbmpl vaccine therapy, n = 10). dogs were submitted to an immunotherapeutic scheme ... | 2017 | 28321217 |
| a library of seleno-compounds and leishmania species: rising evidences towards novel agents. | in vitro leishmanicidal activity of a series of forty-eight selenium derivatives, recently synthesized, was tested on leishmania infantum and leishmania braziliensis parasites, using promastigotes and intracellular amastigote forms. the cytotoxicity of the tested compounds on j774.2 macrophage cells was also measured in order to establish their selectivity. six of the tested compounds (8, 10, 11, 15, 45 and 48) showed selectivity indexes higher than those of the reference drug glucantime for bot ... | 2017 | 28320721 |
| annexin a1 is involved in the resolution of inflammatory responses during leishmania braziliensis infection. | leishmaniases are diseases caused by several leishmania species. leishmania (viannia) braziliensis can cause localized cutaneous leishmaniasis (lcl), which heals spontaneously, or mucosal leishmaniasis (ml), characterized by chronic and intense inflammation and scanty parasitism. annexin a1 (anxa1) is a protein involved in modulation and resolution of inflammation through multiple mechanisms. in the present study, the role of anxa1 was investigated in l. braziliensis-infected balb/c mice. anxa1 ... | 2017 | 28289158 |
| non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and minion™ nanopore sequencing. | cutaneous leishmaniasis (cl) is gaining attention as a public health problem. we present two cases of cl imported from syria and venezuela in japan. we diagnosed them as cl non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the minion™ sequencer. this report demonstrates that our procedure could be useful for the diagnosis of cl in both clinical and epidemiological settings. | 2017 | 28288843 |
| probing the efficacy of a heterologous leishmania/l. viannia braziliensis recombinant enolase as a candidate vaccine to restrict the development of l. infantum in balb/c mice. | in the present study, the leishmania braziliensis enolase protein was evaluated as a vaccine candidate against visceral leishmaniasis (vl). the dna sequence was cloned and the recombinant protein (renolase) was evaluated as a vaccine, associated with saponin, as an immune adjuvant. the protective efficacy of the renolase plus saponin combination was investigated in balb/c mice against leishmania infantum infection. the results revealed that the vaccine induced higher levels of ifn-γ, il-12, and ... | 2017 | 28288798 |
| screening of tnfα, il-10 and tlr4 single nucleotide polymorphisms in individuals with asymptomatic and chronic cutaneous leishmaniasis in colombia: a pilot study. | clinical manifestations of cutaneous leishmaniasis (cl) caused by leishmania (viannia) range from asymptomatic infection to self-limited, or chronic (non-healing) cutaneous lesions. given the critical role of the immune response in the clinical outcome of cl, it is plausible that functional polymorphisms in immune-related genes contribute to define the clinical manifestations of human infection. | 2017 | 28241747 |
| sand fly population dynamics and cutaneous leishmaniasis among soldiers in an atlantic forest remnant in northeastern brazil. | outbreaks of cutaneous leishmaniasis are relatively common among soldiers involved in nocturnal activities in tropical forests. we investigated the population dynamics of sand flies in a military training camp located in a remnant of atlantic rainforest in northeastern brazil, where outbreaks of cutaneous leishmaniasis have sporadically been described. from july 2012 to july 2014, light traps were monthly placed in 10 collection sites, being nine sites located near the forest edge and one near a ... | 2017 | 28241005 |
| american cutaneous leishmaniasis: in situ immune response of patients with recent and late lesions. | tnf-α, ifn-γ, il-10, il-17, cd68, and cd57 were evaluated in biopsies of patients with american cutaneous leishmaniasis living in sorocaba, brazil. the analyses were performed considering the time of lesions from 23 patients with recent lesions (group i) and 19 patients with late lesions (group ii). all patients were infected with leishmania (viannia) braziliensis. immunostaining cells for cd68, cd57, tnf- α, ifn-γ, il-10, and il-17 were performed by immunohistochemistry. except for cd68 and il- ... | 2017 | 28239875 |
| immunopathological characterization of human cutaneous leishmaniasis lesions caused by leishmania (viannia) spp. in amazonian brazil. | american cutaneous leishmaniasis (acl) is a chronic infectious disease caused by different protozoan species of leishmania, and it is endemic in both tropical and subtropical countries. using immunohistochemistry, we investigate the density of cd68(+), lysozyme(+), cd1a(+), factor xiiia(+), cd4(+), cd8(+), cd56(+), interferon (ifn)-γ(+), and inducible no synthase (inos(+)) cells. these cells were analyzed from 22 biopsy samples obtained from the lesions of acl patients, whose infection was cause ... | 2017 | 28224222 |
| the evolutionary loss of rnai key determinants in kinetoplastids as a multiple sporadic phenomenon. | we screened the genomes of a broad panel of kinetoplastid protists for genes encoding proteins associated with the rna interference (rnai) system using probes from the argonaute (ago1), dicer1 (dcl1), and dicer2 (dcl2) genes of leishmania brasiliensis and crithidia fasciculata. we identified homologs for all the three of these genes in the genomes of a subset of these organisms. however, several of these organisms lacked evidence for any of these genes, while others lacked only dcl2. the open re ... | 2017 | 28210761 |
| achievement of constitutive fluorescent plexsy-egfp leishmania braziliensis and its application as an alternative method for drug screening in vitro. | gene reporter-fluorescent cells have emerged as alternative method for drug screening. | 2017 | 28177050 |
| is leishmania (viannia) braziliensis parasite load associated with disease pathogenesis? | leishmania (viannia) braziliensis is the main etiological agent of tegumentary leishmaniasis in the americas. parasite molecular diversity and host immune status contribute to extensive variations in its clinical presentation within endemic areas of brazil. pentavalent antimonials have been used for more than 60 years as the first-line drug for all cases, despite the potential for severe side effects and refractoriness. in rio de janeiro, brazil, most l. (v.) braziliensis infections are benign w ... | 2017 | 28167253 |
| evaluation of a hypothetical protein for serodiagnosis and as a potential marker for post-treatment serological evaluation of tegumentary leishmaniasis patients. | the serodiagnosis for tegumentary leishmaniasis (tl) presents problems related to the sensitivity and/or specificity of the tests. in the present study, an enzyme-linked immunosorbent assay (elisa) technique was used to evaluate the performance from a leishmania braziliensis hypothetical protein, lbhym, in an attempt to compare its serological reactivity with a soluble leishmania antigenic preparation (sla) for the serodiagnosis of cutaneous (cl) and mucosal (ml) leishmaniasis. lbhym was predict ... | 2017 | 28150041 |
| the paraguayan rhinella toad venom: implications in the traditional medicine and proliferation of breast cancer cells. | toads belonging to genus rhinella are used in paraguayan traditional medicine to treat cancer and skin infections. | 2017 | 28131913 |
| design, synthesis, structure-activity relationship and mechanism of action studies of a series of 4-chloro-1-phthalazinyl hydrazones as a potent agent against leishmania braziliensis. | with the aim to identify a potential drug candidate to treat cutaneous leishmaniasis, a series of 1-phthalazinyl hydrazones were synthesized and tested against leishmania braziliensis parasite, one of the main responsible of this disease in the world. a structure-activity relationship permitted to identify two phthalazines containing nitroheterocyclic moiety 3l and 3m as promising new lead compounds. these compounds showed a significant antileishmanial activity against promastigote form of l. br ... | 2017 | 28119201 |
| analysis of expression of fli1 and mmp1 in american cutaneous leishmaniasis caused by leishmania braziliensis infection. | fli1 (friend leukemia virus integration 1) and il6 (interleukin 6; il-6) are associated with leishmania braziliensis susceptibility. cutaneous lesions show exaggerated matrix metalloproteinase 1 (mmp1). in other skin diseases, fli1 promoter methylation reduces fli1 expression, and low fli1 down-regulates mmp1. il-6 increases fli1 expression. we hypothesized that epigenetic regulation of fli1 in cutaneous leishmaniasis, together with il-6, might determine mmp1 expression. while generally low (<10 ... | 2017 | 28119029 |
| characterization of the histopathologic features in patients in the early and late phases of cutaneous leishmaniasis. | cutaneous leishmaniasis (cl), characterized by an ulcerated lesion, is the most common clinical form of human leishmaniasis. before the ulcer develops, patients infected with leishmania (viannia) braziliensis develop a small papule at the site of the sandfly bite, referred to as early cutaneous leishmaniasis (e-cl). two to four weeks later the typical ulcer develops, which is considered here as late cl (l-cl). although there is a great deal known about t-cell responses in patients with l-cl, the ... | 2017 | 28115669 |
| insights on the structural dynamics of leishmania braziliensis hsp90 molecular chaperone by small angle x-ray scattering. | heat shock protein of 90kda (hsp90) is an essential molecular chaperone involved in a plethora of cellular activities which modulate protein homeostasis. during the hsp90 mechanochemical cycle, it undergoes large conformational changes, oscillating between open and closed states. although structural and conformational equilibria of prokaryotic and some eukaryotic hsp90s are known, some protozoa hsp90 structures and dynamics are poorly understood. in this study, we report the solution structure a ... | 2017 | 28104372 |
| antiviral screening identifies adenosine analogs targeting the endogenous dsrna leishmania rna virus 1 (lrv1) pathogenicity factor. | the endogenous double-stranded rna (dsrna) virus leishmaniavirus (lrv1) has been implicated as a pathogenicity factor for leishmaniasis in rodent models and human disease, and associated with drug-treatment failures in leishmania braziliensis and leishmania guyanensis infections. thus, methods targeting lrv1 could have therapeutic benefit. here we screened a panel of antivirals for parasite and lrv1 inhibition, focusing on nucleoside analogs to capitalize on the highly active salvage pathways of ... | 2017 | 28096399 |
| aspects on the ecology of phlebotomine sand flies (diptera: psychodidae) from guaraí, state of tocantins, brazil, endemic area for american cutaneous leishmaniasis. | in brazil, american cutaneous leishmaniasis (acl) ecology involves a diversity of leishmania species transmitted by different sand fly species. workers involved in agricultural activities are those mainly affected by acl in some regions from tocantins state (to), brazil, where the disease can be established in new settlements. the objective of this study was to examine the seasonal and hourly frequency of sand fly species, focusing on the potential vectors of acl, in a settlement in guaraí (to), ... | 2017 | 28082651 |
| molecular detection of leishmania dna in wild-caught phlebotomine sand flies (diptera: psychodidae) from a cave in the state of minas gerais, brazil. | leishmania spp. are distributed throughout the world, and different species are associated with varying degrees of disease severity. in brazil, leishmania transmission involves several species of phlebotomine sand flies that are closely associated with different parasites and reservoirs, and thereby giving rise to different transmission cycles. infection occurs during the bloodmeals of sand flies obtained from a variety of wild and domestic animals, and sometimes from humans. the present study f ... | 2017 | 28082647 |
| nycthemeral rhythm of phlebotominae (diptera: psychodidae) in a craggy region, transitioning between the wetland and the plateau, brazil. | recording the nycthemeral rhythm of sand flies allows the evaluation of the daily activity in different ecotypes, the period of greatest activity, and their degree of anthropophily. we investigated the fauna and the rhythm of sand fly activity in an ecotourism region in mato grosso do sul (ms) state, brazil. sand flies were captured monthly, using a shannon trap for 24 h periods between july 2012 and june 2014. we collected 1,815 sand flies, in which lutzomyia whitmani (=nyssomyia whitmani, sens ... | 2017 | 28082638 |
| phenotypic and functional characteristics of hla-dr(+) neutrophils in brazilians with cutaneous leishmaniasis. | the protozoan leishmania braziliensis causes cutaneous leishmaniasis (cl) in endemic regions. in murine models, neutrophils (pmns) are recruited to the site of infection soon after parasite inoculation. however, the roles of neutrophils during chronic infection and in human disease remain undefined. we hypothesized that neutrophils help maintain a systemic inflammatory state in subjects with cl. lesion biopsies from all patients with cl tested contained neutrophils expressing hla-dr, a molecule ... | 2017 | 28076241 |
| susceptibility to miltefosine in brazilian clinical isolates of leishmania (viannia) braziliensis. | leishmania (viannia) braziliensis is the main causative species of tegumentary leishmaniasis in brazil. in this study, we evaluated the susceptibility of 16 clinical isolates of l. (v.) braziliensis from different regions of brazil to miltefosine in vitro. half-maximal inhibitory concentrations of miltefosine varied from 22.9 to 144.2 μm against promastigotes and from 0.3 to 4.2 μm against intracellular amastigotes. no significant differences were found between isolates of different geographical ... | 2017 | 28070006 |
| local delivery of the toll-like receptor 9 ligand cpg downregulates host immune and inflammatory responses, ameliorating established leishmania (viannia) panamensis chronic infection. | infection by leishmania (viannia) panamensis, the predominant etiologic agent for cutaneous leishmaniasis in colombia, is characterized by a chronic mixed inflammatory response. current treatment options are plagued by toxicity, lengthy treatment regimens, and growing evidence of drug resistance. immunotherapy, modulating the immune system to mount a protective response, may provide an alternate therapeutic approach. we investigated the ability of the toll-like receptor 9 (tlr9) ligand cpg to mo ... | 2017 | 28052994 |
| pharmacokinetics of miltefosine in children and adults with cutaneous leishmaniasis. | an open-label pharmacokinetics (pk) clinical trial was conducted to comparatively assess the pk and explore the pharmacodynamics (pd) of miltefosine in children and adults with cutaneous leishmaniasis (cl) in colombia. sixty patients, 30 children aged 2 to 12 years and 30 adults aged 18 to 60 years, were enrolled. participants received miltefosine (impavido) at a nominal dose of 2.5 mg/kg/day for 28 days. miltefosine concentrations were measured in plasma and peripheral blood mononuclear cells b ... | 2017 | 27956421 |
| field validation of sybr green- and taqman-based real-time pcr using biopsy and swab samples to diagnose american tegumentary leishmaniasis in an area where leishmania (viannia) braziliensis is endemic. | the precise diagnosis of american tegumentary leishmaniasis (atl) is an essential task due to the disease's associated morbidity. a noninvasive, extremely sensitive, and highly specific exam is critical, particularly for mucosal leishmaniasis (ml), in which a low parasite quantity is expected. we aimed to compare the diagnostic accuracy of swab and biopsy sample analysis using sybr green- and taqman-based real-time pcr (qpcr) assays with that of a composite reference standard consisting of the m ... | 2017 | 27927916 |
| increasing in cysteine proteinase b expression and enzymatic activity during in vitro differentiation of leishmania (viannia) braziliensis: first evidence of modulation during morphological transition. | leishmania (viannia) braziliensis presents adaptive protease-dependent mechanisms, as cysteine proteinases b (cpb). this study investigates the expression of three cpb gene isoforms and cpb enzymatic activity during the parasite differentiation. relative expression levels of lbrm.08.0810 gene were assessed, exhibiting a higher quantity of transcripts in the logarithmic promastigotes phase than in the stationary promastigotes phase (>1.5 times). the cbp gene tends to decrease during acid ph shock ... | 2017 | 27919786 |
| leishmaniasis recidivans in ethiopia: cutaneous and mucocutaneous features. | cutaneous leishmaniasis (cl) is endemic in ethiopia. an unusual clinical form of this disease is leishmaniasis recidivans (lr), a prolonged, relapsing form of cutaneous leishmaniasis resembling tuberculosis of the skin that may persist for many years with a chronic and relapsing course. this rare variant has been shown to be caused by leishmania tropica species in the old world and by leishmania braziliensis, leishmania amazonensis, leishmania panamensis, and leishmania guyanensis in the new wor ... | 2017 | 28141598 |
| 5-ala-mediated photodynamic therapy reduces the parasite load in mice infected with leishmania braziliensis. | photodynamic therapy (pdt) has proven to be an effective alternative for the treatment of cutaneous leishmaniasis. skin lesions consist of ulcers with well-defined raised edges, and granular floor. th1 immune response is the protective profile in patients infected with leishmania. in this study, the photodynamic therapy with 5-aminolevulinic acid, the parasitic load, and the modulation of the immune response was evaluated in mice infected with leishmania braziliensis. balb/c mice were infected w ... | 2017 | 27886396 |
| structural and functional studies of the leishmania braziliensis mitochondrial hsp70: similarities and dissimilarities to human orthologues. | heat shock protein 70 kda (hsp70) is a conserved molecular chaperone family involved in several functions related to protein homeostasis. in eukaryotes, hsp70 homologues are found in all cell compartments. the mitochondrial hsp70 isoform (mthsp70) is involved in import of mitochondrial matrix proteins as well as their folding and maturation. moreover, mthsp70 has the propensity to self-aggregate, and it depends on the action of the co-chaperone hsp70-escort protein 1 (hep1) to be produced functi ... | 2017 | 27840097 |
| fluconazole in the treatment of cutaneous leishmaniasis caused by leishmania braziliensis: a randomized controlled trial. | the treatment of cutaneous leishmaniasis (cl) caused by leishmania braziliensis in brazil with pentavalent antimony (sb(v)) is associated with a high rate of failure, up to 45% of cases. in addition, sb(v) can only administered parenterally and has important toxic effect. an effective, safe, and oral treatment for cl is required. | 2017 | 27803094 |
| sclerosing orbital inflammation caused by leishmania braziliensis. | orbital biopsy of nonspecific orbital inflammation, commonly referred to as "orbital pseudotumor," typically shows a combination of polyclonal lymphocytes, plasmocytes, leukocytes, macrophages, and variable degrees of collagen deposition. herein, we report a patient with a positive history of mucocutaneous leishmaniasis who presented with an orbital mass with a histological profile of idiopathic orbital inflammation. immunohistochemical and molecular analysis of the orbital specimens demonstrate ... | 2017 | 27799649 |
| brazilian propolis promotes immunomodulation on human cells from american tegumentar leishmaniasis patients and healthy donors infected with l. braziliensis. | american tegumentar leishmaniasis (atl) is an infectious disease caused by leishmania parasites with ineffective treatment. the properties of propolis have been studied in different experimental studies, however, few works have investigated the effects of propolis on human-derived peripheral blood mononuclear cells (pbmc) in leishmaniasis models. thus, we investigate the immunomodulatory effects of propolis treatment on pbmc from atl patients and on pbmc from healthy donors infected with leishma ... | 2017 | 27702443 |
| leishmania-fast15: a rapid, sensitive and low-cost real-time pcr assay for the detection of leishmania infantum and leishmania braziliensis kinetoplast dna in canine blood samples. | we describe an improved real-time pcr assay (designated as "leishmania-fast15") for the detection and quantification of leishmania infantum and leishmania braziliensis kinetoplast dna minicircles in canine blood samples. the analytical sensitivity of this technique is 0.1 fg of dna, which is equivalent to 0.002 parasite per reaction. this assay uses a small reaction volume (15 μl) and is rapid to perform, with the results being available in less than 34 min. this improved assay might also be sui ... | 2017 | 27554834 |
| low frequency of lrv1 in leishmania braziliensis strains isolated from typical and atypical lesions in the state of minas gerais, brazil. | the double stranded rna (dsrna) virus leishmaniavirus (totiviridae) was first described in leishmania guyanensis and l. braziliensis (lrv1), and more recently from l. major and l. aethiopica (lrv2). parasites bearing lrv1 elicit a higher pro-inflammatory profile, arising through activation of toll like receptor 3(tlr3) interacting with the viral dsrna. lrv1 is most common in leishmania from the amazon region; however data for other regions of brazil are more limited. here we applied pcr tests wi ... | 2017 | 27546549 |
| disseminated cutaneous leishmaniasis in colombia: report of 27 cases. | disseminated leishmaniasis (dl) is a poorly described disease that is frequently misdiagnosed as other clinical manifestations of cutaneous leishmaniasis (cl) such as diffuse cl or post-kala-azar dermal leishmaniasis. twenty-seven cases of dl diagnosed between 1997 and 2015 are described. a higher prevalence was observed in men (mean age 32 years). the number of lesions per patient ranged from 12 to 294, distributed mainly in the upper extremities, face and trunk. the lesions were mostly plaques ... | 2017 | 26557074 |
| two cases of cutaneous leishmaniasis in dutch military personnel treated with oral miltefosine. | in the netherlands, cutaneous leishmaniasis is most commonly seen in military personnel deployed on a mission or training abroad. the treatment of two dutch soldiers who acquired cutaneous leishmaniasis with oral miltefosine was evaluated. adverse effects were monitored and the improvement of skin lesions was assessed. | 2017 | 26661280 |
| genotype characterization of leishmania (viannia) braziliensis isolated from human and canine biopsies with american cutaneous leishmaniasis. | american tegumentary leishmaniasis (atl) can be caused by leishmania (viannia) braziliensis complex. the evolution of atl initially results in lesions and can develop into disseminated or diffuse forms. the genetic diversity of l. (v.) braziliensis in some endemic areas of brazil has been poorly studied, such as in the state of são paulo. this study analyzed the genetic diversity of l. (v.) braziliensis isolates collected from patients and dogs with lta from the state of são paulo. | 2017 | 26200968 |
| cd8+ t cells in situ in different clinical forms of human cutaneous leishmaniasis. | leishmania braziliensis infection induces a large spectrum of lesions that clinically manifest as nodules or papules that progress to ulcers. although it is already known that t helper cells predominate in the lesions, cytotoxic t cells have also been reported to be present, and their role in leishmaniasis immunopathogenesis is not well known. this study investigated the amounts of cd8+ and granzyme b+ cells in different clinical forms of human cutaneous leishmaniasis (cl). | 2017 | 24474014 |
| detection and identification of leishmania spp.: application of two hsp70-based pcr-rflp protocols to clinical samples from the new world. | leishmaniasis is highly prevalent in new world countries, where several methods are available for detection and identification of leishmania spp. two hsp70-based pcr protocols (pcr-n and pcr-f) and their corresponding restriction fragment length polymorphisms (rflp) were applied for detection and identification of leishmania spp. in clinical samples recruited in colombia, guatemala, and honduras. a total of 93 cases were studied. the samples were classified into positive or suspected of leishman ... | 2017 | 28573463 |
| natural leishmania (viannia) infections of phlebotomines (diptera: psychodidae) indicate classical and alternative transmission cycles of american cutaneous leishmaniasis in the guiana shield, brazil. | from 1996 to 1999 multi-trapping methods (center of diseases control, cdc) light traps, light-baited shannon traps, and aspiration on tree bases) were used to study the phlebotomine fauna of the "serra do navio" region of the brazilian state of amapá, which is part of the guiana shield. fifty-three species were identified among 8,685 captured individuals. the following species, associated with the transmission of american cutaneous leishmaniasis in amazonian brazil, were captured: nyssomyia umbr ... | 2017 | 28508745 |
| leishmania (v.) braziliensis infecting bats from pantanal wetland, brazil: first records for platyrrhinus lineatus and artibeus planirostris. | in the new world genus leishmania parasites are etiological agents of neglected zoonoses known as leishmaniasis. its epidemiology is very complex due to the participation of several species of sand fly vectors and mammalian hosts, and man is an accidental host. control is very difficult because of the different epidemiological patterns of transmission observed. studies about leishmania spp. infection in bats are so scarce, which represents a large gap in knowledge about the role of these animals ... | 2017 | 28502644 |
| leishmania infection in bats from a non-endemic region of leishmaniasis in brazil. | leishmaniasis is a complex of zoonotic diseases caused by parasites of the genus leishmania, which can develop in domestic as well as wild animals and humans throughout the world. currently, this disease is spreading in rural and urban areas of non-endemic regions in brazil. recently, bats have gained epidemiological significance in leishmaniasis due to its close relationship with human settlements. in this study, we investigated the presence of leishmania spp. dna in blood samples from 448 bats ... | 2017 | 28831941 |
| anti-leishmania activity of new ruthenium(ii) complexes: effect on parasite-host interaction. | leishmaniasis is a parasitic disease caused by protozoa of the genus leishmania. the many complications presented by the current treatment - including high toxicity, high cost and parasite resistance - make the development of new therapeutic agents indispensable. the present study aims to evaluate the anti-leishmania potential of new ruthenium(ii) complexes, cis‑[ru(ii)(η(2)-o2cr)(dppm)2]pf6, with dppm=bis(diphenylphosphino)methane and r=4-butylbenzoate (bbato) 1, 4-(methylthio)benzoate (mtbato) ... | 2017 | 28783554 |
| il-32γ promotes the healing of murine cutaneous lesions caused by leishmania braziliensis infection in contrast to leishmania amazonensis. | interleukin 32 (il-32) is a pro-inflammatory cytokine induced in patients with american tegumentary leishmaniasis (atl) caused by leishmania braziliensis. here, we investigated whether il-32 is also expressed in patient lesions caused by l. amazonensis. in addition, we evaluated experimental l. amazonensis and l. braziliensis infections in c57bl/6 transgenic mice for human il-32γ (il-32γtg) in comparison with wild-type (wt) mice that do not express the il-32 gene. | 2017 | 28709468 |
| the use of herbs against neglected diseases: evaluation of in vitro leishmanicidal and trypanocidal activity of stryphnodendron rotundifolium mart. | the evaluation of the leishmanicidal and trypanocidal activity of the hydroalcoholic extract of the bark of stryphnodendron rotundifolium mart. (ehcsr) was carried out to find an alternative treatment for parasitic diseases. ehcsr was prepared and used at four different concentrations (1000, 500, 250, 125 μg/ml) in in vitro assays for activity against leishmania promastigotes using the species leishmania brasiliensis and leishmania infantum and for trypanocidal activity using the epimastigotes o ... | 2017 | 28855804 |
| profiling gene expression of antimony response genes in leishmania (viannia) panamensis and infected macrophages and its relationship with drug susceptibility. | the mechanisms of leishmania resistance to antimonials have been primarily determined in experimentally derived leishmania strains. however, their participation in the susceptibility phenotype in field isolates has not been conclusively established. being an intracellular parasite, the activity of antileishmanials is dependent on internalization of drugs into host cells and effective delivery to the intracellular compartments inhabited by the parasite. in this study we quantified and comparative ... | 2017 | 28843396 |
| platelet-activating factor increases reactive oxygen species-mediated microbicidal activity of human macrophages infected with leishmania (viannia) braziliensis. | platelet-activating factor (paf) is produced by macrophages during inflammation and infections. we evaluated whether paf is able to modulate the infection of human macrophages by leishmania braziliensis, the main leishmania sp. in brazil. monocyte-derived macrophages were incubated with promastigote forms in absence or presence of exogenous paf. we observed that the treatment of macrophages with low concentrations of paf prior to infection increased the phagocytosis of l. braziliensis. more impo ... | 2017 | 28830073 |
| leishmania (viannia) braziliensis inositol phosphorylceramide: distinctive sphingoid base composition. | inositol phosphorylceramide (ipc), the major sphingolipid in the genus leishmania but not found in mammals, is considered a potentially useful target for chemotherapy against leishmaniasis. leishmania (viannia) braziliensis is endemic in latin america and causes american tegumentary leishmaniasis. we demonstrated that ipcs are localized internally in parasites, using a specific monoclonal antibody. treatment with 5 μm myriocin (a serine palmitoyltransferase inhibitor) rendered promastigotes 8-fo ... | 2017 | 28824583 |
| cutaneous leishmaniosis in naturally infected dogs in paraná, brazil, and the epidemiological implications of leishmania (viannia) braziliensis detection in internal organs and intact skin. | environmental changes have occurred over the years, altering the eco-epidemiological pattern of leishmaniosis in the state of paraná, brazil, involving the pillars of the cycle (parasite, vectors, reservoir, and environment) and their interaction. much has been discussed about the dog's role as a reservoir of the leishmania (viannia) braziliensis vianna, 1911 transmission cycle. however, this question remains unanswered. the purpose of this study was to investigate, using parasitological and mol ... | 2017 | 28807297 |
| sensitive molecular diagnostics for cutaneous leishmaniasis. | rapid diagnosis of cutaneous leishmaniasis (cl) and identification of leishmania species is highly important for the disease management. in israel, cl is caused mainly by leishmania major and leishmania tropica species. | 2017 | 28795077 |
| clinical and laboratory profiles of patients with early spontaneous healing in cutaneous localized leishmaniasis: a historical cohort study. | skin ulcers in american cutaneous leishmaniasis (acl) may heal spontaneously after months/years. however, few cases may present quick heal even during diagnosis procedure (early spontaneous healing- esh). the main objective of this study was to compare esh patients with cases requiring specific treatment [non-esh (nesh)]. | 2017 | 28793868 |
| in vitro antileishmanial activity and iron superoxide dismutase inhibition of arylamine mannich base derivatives. | leishmaniasis is one of the world's most neglected diseases, and it has a worldwide prevalence of 12 million. there are no effective human vaccines for its prevention, and treatment is hampered by outdated drugs. therefore, research aiming at the development of new therapeutic tools to fight leishmaniasis remains a crucial goal today. with this purpose in mind, we present 20 arylaminoketone derivatives with a very interesting in vitro and in vivo efficacy against trypanosoma cruzi that have now ... | 2017 | 28789716 |
| synthetic peptides derived from ribosomal proteins of leishmania spp. in mucocutaneous leishmaniasis: diagnostic usefulness. | the serological diagnostic methods currently available for mucocutaneous leishmaniasis (mcl) lack specificity when complete parasites are used; however, such specificity increases when protein fractions are used. ribosomal proteins have been reported to induce antibodies in animal and humans infected with the parasite, making them a worth candidate to assess its diagnosis potential. this study was thus aimed at evaluating synthetic peptides derived from leishmania braziliensis ribosomal proteins ... | 2017 | 28758598 |
| leishmanicidal effect of antiparasitic photodynamic therapy-appdt on infected macrophages. | the aim of this study is to evaluate the effects of appdt (antiparasitic photodynamic therapy) on the interaction of leishmania braziliensis with j774 macrophages, used as a photosensitizer, methylene blue associated with red laser. the tests are in triplicate and the samples divided into four groups: control, photosensitizer, laser, and appdt. the photosensitizer used was the methylene blue at concentration of 12.5 μg/ml. the parameters of the laser were λ = 660 nm, 40 mw, and 8.4 j/cm(2). samp ... | 2017 | 28752261 |
| leishmaniasis in the major endemic region of plurinational state of bolivia: species identification, phylogeography and drug susceptibility implications. | the plurinational state of bolivia is one of the latin american countries with the highest prevalence of leishmaniasis, highlighting the lowlands of the department of la paz where about 50% of the total cases were reported. the control of the disease can be seriously compromised by the intrinsic variability of the circulating species that may limit the efficacy of treatment while favoring the emergence of resistance. fifty-five isolates of leishmania from cutaneous and mucocutaneous lesions from ... | 2017 | 28751163 |
| macromolecular biosynthetic parameters and metabolic profile in different life stages of leishmania braziliensis: amastigotes as a functionally less active stage. | it was recently hypothesized that leishmania amastigotes could constitute a semi-quiescent stage characterized by low replication and reduced metabolic activity. this concept developed with leishmania (leishmania) mexicana and leishmania (leishmania) major models might explain numerous clinical and sub-clinical features of leishmania (viannia) braziliensis infections, like reactivation of the disease, non-response to chemotherapy or asymptomatic infections. we compared here in vitro the prolifer ... | 2017 | 28742826 |
| mucosal leishmaniasis: a retrospective study of 327 cases from an endemic area of leishmania (viannia) braziliensis. | mucosal leishmaniasis (ml) is observed only in about 3% of patients with american tegumentary leishmaniasis (atl) but has a high potential for destructive, disfiguring, and disabling sequelae. prior reports of clinical and epidemiologic features of ml are limited by small numbers of cases. in this study, we evaluated changes in the demographic features and clinical presentation of ml in an endemic area of leishmania braziliensis transmission over a period of 20 years. the charts of 327 patients ... | 2017 | 28722607 |
| parasite killing of leishmania (v) braziliensis by standardized propolis extracts. | treatments based on antimonials to cutaneous leishmaniasis (cl) entail a range of toxic side effects. propolis, a natural compound widely used in traditional medical applications, exhibits a range of biological effects, including activity against infectious agents. the aim of this study was to test the potential leishmanicidal effects of different propolis extracts against leishmania (viannia) braziliensis promastigotes and intracellular amastigotes in vitro. stationary-phase l. (v) braziliensis ... | 2017 | 28690662 |
| rapid detection of trypanosoma cruzi by colorimetric loop-mediated isothermal amplification (lamp): a potential novel tool for the detection of congenital chagas infection. | early diagnosis of congenital trypanosomacruzi transmission in newborns is essential because babies show high indices of cure. conventional diagnosis is based on microscopic examination and serology. molecular diagnosis is a promising alternative to replace conventional diagnosis, although it is not well suited for adoption in laboratories with limited resources. isothermal dna amplification methods have the advantage of not requiring expensive equipment. the aim of this work was to apply loop-m ... | 2017 | 28684052 |
| cutaneous leishmaniasis induces a transmissible dysbiotic skin microbiota that promotes skin inflammation. | skin microbiota can impact allergic and autoimmune responses, wound healing, and anti-microbial defense. we investigated the role of skin microbiota in cutaneous leishmaniasis and found that human patients infected with leishmania braziliensis develop dysbiotic skin microbiota, characterized by increases in the abundance of staphylococcus and/or streptococcus. mice infected with l. major exhibit similar changes depending upon disease severity. importantly, this dysbiosis is not limited to the le ... | 2017 | 28669672 |
| seroconversion to lutzomyia intermedia linb-13 as a biomarker for developing cutaneous leishmaniasis. | sand flies inject saliva while feeding in the vertebrate host and anti-saliva antibodies can be used as biomarkers of exposure to leishmania vectors. we expressed recombinant salivary proteins from lutzomyia intermedia, a vector of leishmania braziliensis, and evaluated the seroreactivity in exposed individuals in search for exposure markers. we found a strong correlation among positive serology to recombinant proteins linb-13, 26, 15, 21 and to salivary proteins: rlinb-13 was the top performing ... | 2017 | 28600554 |
| trichophoromyia auraensis is a putative vectorcomments about the finding of trichophoromyia auraensis (mangabeira 1942) sandfly specimens infected by leishmania (viannia) parasites in acre statea new sandfly in the subgenus nyssomyia (diptera, psychodidae) from the amazon basin of brazilthe known geographical distribution of sandflies in the state of acre, brazil (diptera: psychodidae)studies on the sandfly fauna (diptera: psychodidae: phlebotominae) from transmission areas of american cutaneous leishmaniasis in state of acre, brazildevelopment and validation of pcr-based assays for diagnosis of american cutaneous leishmaniasis and identification of the parasite speciesfirst description of leishmania (viannia) infection in evandromyia saulensis, pressatia sp. and trichophoromyia auraensis (psychodidae: phlebotominae) in a transmission area of cutaneous leishmaniasis in acre state, amazon basin, brazilsand fly fauna (diptera: psychodidae: phlebotominae) in an area of leishmaniasis transmission in the municipality of rio branco, state of acre, brazilidentification of naturally infected lutzomyia intermedia and lutzomyia migonei with leishmania (viannia) braziliensis in rio de janeiro (brazil) revealed by a pcr multiplex non-isotopic hybridization assaynotas sobre os flebotomíneos do estado do acre, com a descrição de duas espécies novas (diptera, psychodidae)description of trichophoromyia ruifreitasi, a new phlebotomine species (diptera, psychodidae) from acre state, brazilian amazonepidemiologia da leishmaniose tegumentar e descrição das populações de flebotomíneos no município de acrelândia, acre, brasilphlebotomine sandfly (diptera: psychodidae) diversity and their leishmania dna in a hot spot of american cutaneous leishmaniasis human cases along the brazilian border with peru and boliviadescription of a new phlebotomine species (diptera: psychodidae, phlebotominae) and new records of sand flies from the state of acre, northern brazilnatural leishmania infection of lutzomyia (trichophoromyia) auraensis in madre de dios, peru, detected by a fluorescence resonance energy transfer-based real-time polymerase chain reactionguide to the identification and geographic distribution of lutzomyia sandflies in mexico, the west indies, central and south america (diptera: psychodidae). | the sandfly trichophoromyia auraensis has recently evolved as a proven vector of leishmania (viannia) endemic to state of acre in the north of brazil. this note is intended to propose a correction in the report of the first occurrence of natural infection of leishmania (viannia) in this species. we and the other scientific groups reinforced that tr. auraensis is a possible vector involved in the transmission of american cutaneous leishmaniasis in acre, brazil. | 2017 | 28591315 |
| disseminated cutaneous leishmaniasis caused by leishmania braziliensis in southern brazil. | the authors report a case of disseminated cutaneous leishmaniasis caused by leishmania (viannia) braziliensis, in a 55 years old patient with 1,119 lesions distributed throughout the body. the patient resides in sabáudia municipality, north of paraná state, southern brazil, where there was no previous report of this form of leishmaniasis. treatment with meglumine antimoniate was successful, although the diagnosis was made only five months later. | 2017 | 28591265 |
| sporotrichoid leishmaniasis: a cross-sectional clinical, epidemiological and laboratory study in rio de janeiro state, brazil. | atypical presentations of cutaneous leishmaniasis include sporotrichoid leishmaniasis (sl), which is clinically described as a primary ulcer combined with lymphangitis and nodules and/or ulcerated lesions along its pathway. | 2017 | 28591261 |
| aspects of the ecology of phlebotomine sand flies (diptera: psychodidae) in the private natural heritage reserve sanctuary caraça. | leishmaniases are a set of parasitic diseases of zoonotic origin that are transmitted by sandfly vectors in wild, rural and urban environments. their distribution is dependent not only the distribution of vectors, but also on the distribution of mammalian reservoirs. only by understanding the transmission cycle of these diseases, such as knowing the participating vectors and reservoirs, can one can understand the epidemiology and ecological relationships of leishmaniases. ecotourism has become a ... | 2017 | 28570640 |
| low versus high dose of antimony for american cutaneous leishmaniasis: a randomized controlled blind non-inferiority trial in rio de janeiro, brazil. | although high dose of antimony is the mainstay for treatment of american cutaneous leishmaniasis (acl), ongoing major concerns remain over its toxicity. whether or not low dose antimony regimens provide non-inferior effectiveness and lower toxicity has long been a question of dispute. | 2017 | 28558061 |
| the use of kdna minicircle subclass relative abundance to differentiate between leishmania (l.) infantum and leishmania (l.) amazonensis. | leishmaniasis is a neglected disease caused by many leishmania species, belonging to subgenera leishmania (leishmania) and leishmania (viannia). several qpcr-based molecular diagnostic approaches have been reported for detection and quantification of leishmania species. many of these approaches use the kinetoplast dna (kdna) minicircles as the target sequence. these assays had potential cross-species amplification, due to sequence similarity between leishmania species. previous works demonstrate ... | 2017 | 28511704 |
| oral pentoxifylline associated with pentavalent antimony: a randomized trial for cutaneous leishmaniasis. | abstractcutaneous leishmaniasis (cl) by leishmania braziliensis is associated with decreasing cure rates in brazil. standard treatment with pentavalent antimony (sb(v)) cures only 50-60% of the cases. the immunopathogenesis of cl ulcer is associated with high interferon-γ and tumor necrosis factor (tnf) production. pentoxifylline, a tnf inhibitor, has been successfully used in association with sb(v) in mucosal and cutaneous leishmaniasis. this randomized, double-blind, and placebo-controlled tri ... | 2017 | 28500815 |
| evaluation of a multilocus sequence typing (mlst) scheme for leishmania (viannia) braziliensis and leishmania (viannia) panamensis in colombia. | leishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. in colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by l. panamensis and l. braziliensis. currently, the genetic diversity of these species in colombia is unknown. to address this, we applied molecular techniques for their characterization, using multilocus sequence typing (mlst) to explore the genetic variability and phylodynamics o ... | 2017 | 28499458 |
| nerolidol, the main constituent of piper aduncum essential oil, has anti-leishmania braziliensis activity. | leishmania (viannia) braziliensis is a protozoan that causes mucocutaneous leishmaniasis, which is an infectious disease that affects more than 12 million people worldwide. the available treatment is limited, has side-effects or is inefficient. in a search for alternative compounds of natural origin, we tested the microbicidal activity of piper aduncum essential oil (paeo) on this parasite. our data showed that paeo had an inhibitory effect on the growth of l. braziliensis promastigotes with an ... | 2017 | 28482935 |
| multi-anti-parasitic activity of arylidene ketones and thiazolidene hydrazines against trypanosoma cruzi and leishmania spp. | a series of fifty arylideneketones and thiazolidenehydrazines was evaluated against leishmania infantum and leishmania braziliensis. furthermore, new simplified thiazolidenehydrazine derivatives were evaluated against trypanosoma cruzi. the cytotoxicity of the active compounds on non-infected fibroblasts or macrophages was established in vitro to evaluate the selectivity of their anti-parasitic effects. seven thiazolidenehydrazine derivatives and ten arylideneketones had good activity against th ... | 2017 | 28481276 |
| in vitro inhibition of leishmania attachment to sandfly midguts and ll-5 cells by divalent metal chelators, anti-gp63 and phosphoglycans. | leishmania braziliensis and leishmania infantum are the causative agents of cutaneous and visceral leishmaniasis, respectively. several aspects of the vector-parasite interaction involving gp63 and phosphoglycans have been individually assayed in different studies. however, their role under the same experimental conditions was not studied yet. here, the roles of divalent metal chelators, anti-gp63 antibodies and purified type i phosphoglycans (pgs) were evaluated during in vitro parasite attachm ... | 2017 | 28472733 |
| cd3(+)cd4(neg)cd8(neg) (double negative) t lymphocytes and nkt cells as the main cytotoxic-related-cd107a(+) cells in lesions of cutaneous leishmaniasis caused by leishmania (viannia) braziliensis. | cutaneous leishmaniasis (cl) is caused by leishmania (viannia) braziliensis, which infects dermal macrophages and dendritic cells, causing an intense immune-mediated-tissue inflammation and a skin ulcer with elevated borders that can heal spontaneously or after antimonial therapy. the resolution of lesions depends on an adaptive immune response, and cytotoxic cells seem to have a fundamental role in this process. the aim of this study is to better understand the role of cytotoxicity mediated mec ... | 2017 | 28468680 |
| correction: meta-transcriptome profiling of the human-leishmania braziliensis cutaneous lesion. | [this corrects the article doi: 10.1371/journal.pntd.0004992.]. | 2017 | 28467433 |
| knockdown of host antioxidant defense genes enhances the effect of glucantime on intracellular leishmania braziliensis in human macrophages. | leishmaniasis is a neglected tropical disease that affects millions of people worldwide and represents a major public health problem. information on protein expression patterns and functional roles within the context of leishmania-infected human monocyte-derived macrophages (mdms) under drug treatment conditions is essential for understanding the role of these cells in leishmaniasis treatment. we analyzed functional changes in the expression of human mdm genes and proteins during in vitro infect ... | 2017 | 28461312 |
| evaluation of methylene blue as photosensitizer in promastigotes of leishmania major and leishmania braziliensis. | the cutaneous leishmaniasis is caused by the protozoan of the genus leishmania. it is considered by who as a public health issue and a neglected disease, which affects rural workers and it is also a risk to travelers in endemic areas. the conventional treatment is toxic and leads to severe side effects. the photodynamic therapy has been studied as an alternative treatment to cutaneous leishmaniasis. this study aimed to evaluate the methylene blue internalization and the impact of the pdt in the ... | 2017 | 28457848 |
| aim2 inflammasome is associated with disease severity in tegumentary leishmaniasis caused by leishmania (v.) braziliensis. | the inflammasome is a multiprotein signalling platform involved in the pathogenesis of various inflammatory skin diseases. herein, we investigated gene and protein expression of the inflammasome molecules aim2 and nlrp3 in active lesions from patients with l. (v.) braziliensis-associated tegumentary leishmaniasis (tl) and correlated these findings with the clinical presentations and responses to therapy. real-time pcr assays showed a significantly higher aim2 gene expression in mucosal leishmani ... | 2017 | 28397969 |
| biophysical and pharmacological characterization of energy-dependent efflux of sb in laboratory-selected resistant strains of leishmania (viannia) subgenus. | the growing resistance of leishmaniasis to first-line drugs like antimonials in some regions limits the control of this parasitic disease. the precise mechanisms involved in leishmania antimony resistance are still subject to debate. the reduction of intracellular sb(iii) accumulation is a common change observed in both laboratory-selected and field isolated resistant leishmania strains, but the exact transport pathways involved in antimony resistance have not yet been elucidated. in order to fu ... | 2017 | 28393067 |
| [cutaneous leishmaniasis in israel 2016 - an update]. | leishmaniasis is endemic in israel and is increasing in its frequency. the pathogens that cause cutaneous leishmaniasis (cl) in israel are leishmania major (l. major) and leishmania tropica (l. tropica). in recent years, there have been significant changes in the distribution of these species. l. major is endemic mainly in the negev, arava and the beit she'an valley. l. tropica is endemic in tiberias, northern sea of galilee, samaria and in the vicinity of jerusalem such as at kfar adumim, ma'al ... | 2016 | 28530056 |
| cell accumulation and antileishmanial effect of exogenous and endogenous protoporphyrin ix after photodynamic treatment. | photodynamic therapy (pdt) using 5-aminolevulinic acid-induced protoporphyrin ix (ala-ppix) constitutes an interesting alternative for cutaneous leishmaniasis treatment. | 2016 | 25504248 |
| study of cutaneous leishmaniasis in the state of campeche (yucatan peninsula), mexico, over a period of two years. | to study cutaneous leishmaniasis (cl), in the calakmul municipality of the campeche state, during two years. | 2016 | 25629280 |
| mucosal relapse one year after complete remission of cutaneous sporotrichoid leishmaniasis due to leishmania braziliensis. | 2016 | 26462596 |