Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| saprotrophic soil fungi to improve phosphorus solubilisation and release: in vitro abilities of several species. | modern agriculture is dependent on phosphate rock (pr), which is a nonrenewable resource. improvement of phosphorus (p) availability for crops in agricultural soils represents a key strategy to slow down the depletion of pr. the aim of this study was to identify potential p biofertilisers among saprotrophic fungal species. we tested 30 fungal strains belonging to 28 taxa (4 zygomycota and 24 ascomycota) and with different life strategies. the study showed that many saprotrophic fungi have the ab ... | 2018 | 29159452 |
| carvacrol and eugenol effectively inhibit rhizopus stolonifer and control postharvest soft rot decay in peaches. | this study aimed to investigate the antifungal mechanism of carvacrol and eugenol to inhibit rhizopus stolonifer and the control of postharvest soft rot decay in peaches. | 2018 | 29044849 |
| synthesis, characterization and antimicrobial activities of copper derivatives of nhc-ii complexes. | caffeine, 1, 3, 7-trimethylxanthine is one of the xanthine derivatives that are for the most part utilized as a part of solutions as diuretics. the cu (ii) complexes have been synthesized from the n-heterocyclic carbene ligands. | 2017 | 29022998 |
| growth modeling to control (in vitro) fusarium verticillioides and rhizopus stolonifer with thymol and carvacrol. | the aim of this study was to evaluate the antifungal activity (in vitro) of thymol and carvacrol alone or in mixtures against fusarium verticillioides and rhizopus stolonifer, and to obtain primary growth models. minimal inhibitory concentration (mic) was evaluated with fungal radial growth with thymol or carvacrol concentrations (0-1600mg/l). mixtures were evaluated using concentrations below mic values. radial growth curves were described by the modified gompertz equation. mic values of carvac ... | 2017 | 28947087 |
| physiological effects and mode of action of zno nanoparticles against postharvest fungal contaminants. | increasing concerns continue to be expressed about health hazards and environmental pollution resulting from the use of conventional fungicides for postharvest disease control. nanoparticles represent an alternative solution for postharvest disease management. the objective of this work was to assess the physiological effects and the antifungal efficiency of zno nanoparticles (zno nps) against a number of fungal contaminants. the efficacy of zno nps was qualitatively and quantitatively assessed ... | 2017 | 28941694 |
| self-induction system for cellulase production by cellobiose produced from glucose in rhizopus stolonifer. | cellulolytic fungi have evolved a sophisticated genetic regulatory network of cellulase synthesis to adapt to the natural environment. even in the absence of lignocellulose, it still secretes low levels of "constitutive" cellulase for standby application. however, the mechanisms of this constitutive expression remain incompletely understood. here we identified a cellobiose synthetase (cbs) from rhizopus stolonifer, which has the capacity to catalyse the synthesis of cellobiose from uridine dipho ... | 2017 | 28860637 |
| reveromycins a and b from streptomyces sp. 3-10: antifungal activity against plant pathogenic fungi in vitro and in a strawberry food model system. | this study was conducted to determine the antifungal activity of the metabolites from streptomyces sp. 3-10, and to purify and identify the metabolites. meanwhile, the taxonomic status of strain 3-10 was re-evaluated. the cultural filtrates of strain 3-10 in potato dextrose broth were extracted with ethyl acetate. the resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and oomycetes, accounting for 92% of the total 24 tested species, sug ... | 2017 | 28421050 |
| isolation and identification of fungi associated with spoilt fruits vended in gwagwalada market, abuja, nigeria. | annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. this study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in gwagwalada market, abuja, and recommend appropriate control measure. | 2017 | 28507410 |
| assessing the anti-fungal efficiency of filters coated with zinc oxide nanoparticles. | air filters support fungal growth, leading to generation of conidia and volatile organic compounds, causing allergies, infections and food spoilage. filters that inhibit fungi are therefore necessary. zinc oxide (zno) nanoparticles have anti-fungal properties and therefore are good candidates for inhibiting growth. two concentrations (0.012 m and 0.12 m) were used to coat two types of filters (melt-blown and needle-punched) for three different periods (0.5, 5 and 50 min). rhizopus stolonifer and ... | 2017 | 28572995 |
| antifungal activity of the pygidial gland secretion of laemostenus punctatus (coleoptera: carabidae) against cave-dwelling micromycetes. | the antifungal potential of the pygidial gland secretion of the troglophilic ground beetle laemostenus punctatus from a cave in southeastern serbia against cave-dwelling micromycetes, isolated from the same habitat, has been investigated. eleven collected samples were analyzed and 32 isolates of cave-dwelling fungi were documented. a total of 14 fungal species were identified as members of the genera aspergillus, penicillium, alternaria, cladosporium, rhizopus, trichoderma, arthrinium, aureobasi ... | 2017 | 28584964 |
| activity of the pterophyllins 2 and 4 against postharvest fruit pathogenic fungi. comparison with a synthetic analog and related intermediates. | the antifungal activity of pterophyllin 2, pterophyllin 4, a 5-desmethyl analog of the latter and some of their synthetic intermediates, against three postharvest phytopathogenic fungi, was evaluated. the target fungi were rhizopus stolonifer, botrytis cinerea and monilinia fructicola, which affect fruits worldwide, causing important economic losses. the tests were carried out with imazalil and carbendazim as positive controls. minimum inhibitory concentrations and minimum fungicidal concentrati ... | 2017 | 29288027 |
| mixed cropping regimes promote the soil fungal community under zero tillage. | fungi of yield soils represent a significant portion of the microbial biomass and reflect sensitivity to changes in the ecosystem. our hypothesis was that crops included in cropping regimes under the zero tillage system modify the structure of the soil fungi community. conventional and molecular techniques provide complementary information for the analysis of diversity of fungal species and successful information to accept our hypothesis. the composition of the fungal community varied according ... | 2017 | 29270767 |
| matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens. | filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (frs). current laboratory diagnosis of frs pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. although matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms has been employed to identify various fungi, its efficacy in the identification of frs fungi is less clear. | 2017 | 28100314 |
| essential oil composition and antifungal activity of melissa officinalis originating from north-est morocco, against postharvest phytopathogenic fungi in apples. | to investigate biological control methods against post-harvest phytopathogenic fungi in apples, tests on the antifungal activity of essential oil of melissa officinalis were carried out. the essential oil, obtained by hydrodistillation, was analyzed by gas chromatography coupled with mass spectrometry (gc-ms). analysis of the essential oil was able to detect 88.7% of the components. the main components are p-mentha-1,2,3-triol (13.1%), p-menth-3-en-8-ol (8.8%), pulegone (8.8%), piperitynone oxid ... | 2017 | 28389346 |
| extracellular biosynthesis of silver nanoparticles using rhizopus stolonifer. | synthesis of silver nanoparticles (agnps) has become a necessary field of applied science. biological method for synthesis of agnps by rhizopus stolonifer aqueous mycelial extract was used. the agnps were identified by uv-visible spectrometry, x-ray diffraction (xrd), transmission electron microscopy (tem) and fourier transform infrared spectrometry (ft-ir). the presence of surface plasmon band around 420 nm indicates agnps formation. the characteristic of the agnps within the face-centered cubi ... | 2017 | 28053592 |
| production of cellulases by rhizopus stolonifer from glucose-containing media based on the regulation of transcriptional regulator cre. | carbon catabolite repression is a crucial regulation mechanism in microorganisms, but its characteristic in rhizopus is still unclear. we extracted a carbon regulation gene, cre, that encoded a carbon catabolite repressor protein (cre) from rhizopus stolonifer tp-02, and studied the regulation of cre by real-time qpcr. cre responded to glucose in a certain range, where it could significantly regulate part of the cellulase genes (eg, bg, and cbh2) without cbh1. in the comparison of the response o ... | 2017 | 27880962 |
| rhizopus stolonifer mediated biosynthesis of biocompatible cadmium chalcogenide quantum dots. | we report an efficient method to biosynthesize biocompatible cadmium telluride and cadmium sulphide quantum dots from the fungus rhizopus stolonifer. the suspension of the quantum dots exhibited purple and greenish-blue luminescence respectively upon uv light illumination. photoluminescence spectroscopy, x-ray diffraction, and transmission electron microscopy confirms the formation of the quantum dots. from the photoluminescence spectrum the emission maxima is found to be 424 and 476nm respectiv ... | 2016 | 27866619 |
| intergeneric fusant development using chitinase preparation of rhizopus stolonifer ncim 880. | fungal chitinase have tremendous applications in biotech industries, with this approach we focused on extracellular chitinase from rhizopus stolonifer ncim 880 for the formation of fungal protoplasts. the maximum chitinase production reached after 24 h at 2.5% colloidal chitin concentration in presence of starch as an inducer. chitinase was extracted efficiently at 65% cold acetone concentration and then purified by using deae-cellulose column chromatography. purified chitinase having molecular ... | 2016 | 27844458 |
| immune-enhancing activity of extracellular polysaccharides isolated from rhizopus nigricans. | extracellular polysaccharides (eps1-1) was extracted from fermentation liquor of rhizopus nigricans and evaluated its immune-enhancing activities in vitro and in vivo. results suggested that the proliferation of lymphocyte was stimulated after treated with eps1-1. moreover, the activities of macrophages were enhanced by increasing the activities of phagocytosis and acid phosphatase, the production of no and the mrna levels of il-2, tnf-α and inos. furthermore, eps1-1 could significantly boost th ... | 2016 | 27185145 |
| anti-tumor and immunomodulatory activities of an exopolysaccharide from rhizopus nigricans on ct26 tumor-bearing mice. | this study was aimed to investigate the anti-tumor and immunomodulatory activities of an exopolysaccharide (eps) from rhizopus nigricans. our results showed eps could significantly inhibit the tumor growth and increase the immune organs index of ct26 tumor-bearing mice. eps treatment increased the productions of interleukin-2 (il-2) and tumor necrosis factor-α (tnf-α) levels in serum. the increase of percentage of cd8(+) cytotoxic t cells among total spleen t lymphocyte was also observed. furthe ... | 2016 | 27163210 |
| anti-tumor activity of exopolysaccharide from rhizopus nigricans ehrenb on s180 tumor-bearing mice. | in this study, the effect of antitumor and immune activities of extracellular polysaccharides (eps) from rhizopus nigricans ehrenb were investigated using s180 bearing mice. the results revealed that eps in the concentration range 50-1000 μg/ml can inhibited s180 cell proliferation in a dose dependent manner. eps at the highest dose of 1000 μg/ml showed significantly antitumor activity against s180 with inhibition rate of 47.53%. however, eps significantly simulated spleen lymphocytes in the con ... | 2016 | 26951752 |
| effect on tomato plant and fruit of the application of biopolymer-oregano essential oil coatings. | oregano essential oil (eo) was incorporated into film-forming dispersions (ffds) based on biopolymers (chitosan and/or methylcellulose) at two different concentrations. the effect of the application of the ffds was evaluated on tomato plants (cultivar micro-tom) at three different stages of development, and on pre-harvest and postharvest applications on tomato fruit. | 2016 | 26869236 |
| purification, partial characterization and antitumor effect of an exopolysaccharide from rhizopus nigricans. | in this study, a homogeneous exopolysaccharide (eps1-1) was purified from the fermentation broth of rhizopus nigricans. eps1-1 was composed of glucose, mannose, galactose and fructose in the molar ratio of 5.89:3.64:3.20:1.00 with weight average molecular weight of 9.7×10(3)g/mol. eps1-1 could significantly inhibit proliferation of human colorectal carcinoma hct-116 cells in vitro. eps1-1 also induced s phase cell cycle arrest and increased sub-g0/g1 population, a hallmark of apoptosis. the resu ... | 2016 | 26449531 |
| antifungal activity of zataria multiflora essential oil-loaded solid lipid nanoparticles in-vitro condition. | the aim of the present study was to prepare, characterize, and evaluate solid lipid nanoparticles (slns) containing zataria multiflora essential oil (zeo). | 2016 | 27917280 |
| fungal biodeterioration, aflatoxin contamination, and nutrient value of "suya spices". | this work aimed to analyze the nutrient values, examine the biodeteriorating fungi biota, and analyze the mycotoxin contents of "suya spices." fungi with highest percentage occurrence on all the samples are aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus ochraceus, fusarium sp., rhizopus stolonifer, yeast, and trichoderma koningii. nutrient composition of the samples is significantly different statistically (p < 0.05) with high protein (9.53% to 13.17%), fiber (9.27 t ... | 2016 | 27092289 |
| microbial diversity in the gut of cashew stem girdler, analeptes trifasciata fabricius (coleoptera: cerambycidae), in ibadan, nigeria. | the cashew stem girdler, analeptes trifasciata, is a major insect pest of cashew in nigeria causing economic damage in cashew plantations even at low density. in this study, newly emerged adults of a. trifasciata reared from field-infested cashew stems were collected from the rearing cages, sexed, and dissected to reveal the internal structures of the insects. the gut was excised and separated into the foregut, midgut, and hindgut. the dissected gut compartments were blotted dry by sandwiching i ... | 2016 | 27147898 |
| effect of plant extracts on book deteriorated fungal species. | the aim of the study was to evaluate the effect of leaf extracts of four plants against some isolated fungal species from deteriorated books. aqueous, methanol and chloroform extracts of selected plant species were screened in vitro for their antifungal activity against some book deteriorating fungal species. fifteen species belonging to 09 genera were isolated and identified from infested books in library. aqueous and solvent extracts of leaves of azadiracta indica, callistemon citrinus, eucaly ... | 2016 | 27154063 |
| new anti-inflammatory metabolites by microbial transformation of medrysone. | microbial transformation of the anti-inflammatory steroid medrysone (1) was carried out for the first time with the filamentous fungi cunninghamella blakesleeana (atcc 8688a), neurospora crassa (atcc 18419), and rhizopus stolonifer (tsy 0471). the objective was to evaluate the anti-inflammatory potential of the substrate (1) and its metabolites. this yielded seven new metabolites, 14α-hydroxy-6α-methylpregn-4-ene-3,11,20-trione (2), 6β-hydroxy-6α-methylpregn-4-ene-3,11,20-trione (3), 15β-hydroxy ... | 2016 | 27104348 |
| effects of poplar buds as an alternative to propolis on postharvest diseases control of strawberry fruits. | botrytis cinerea and rhizopus stolonifer, two main postharvest pathogens, cause great loss of strawberry fruits. here, the effects of poplar buds extracts, a main plant source for chinese propolis, on disease control were investigated in vitro and in vivo. | 2016 | 26140351 |
| screening for antimicrobial activity of wood rotting higher basidiomycetes mushrooms from uruguay against phytopathogens. | in this work, the antimicrobial activity of extracts of wood rotting higher basidiomycetes mushrooms isolated from eucalyptus plantations in uruguay was studied using bacterial and fungal phytopathogens as targets. fifty-one extracts from mycelia and growth broth were prepared from higher basidiomycetes mushrooms, from which eight extracts (from ganoderma resinaceum, laetiporus sulphureus, dictyopanus pusillus, and bjerkandera adusta) showed antimicrobial activity against xanthomonas vesicatoria ... | 2016 | 27481160 |
| mycologic endocrinology. | the interactions of fungi and chemical messenger molecules, hormones or pheromones, are addressed in this chapter. these interactions include mammalian fungal pathogens, also plant pathogens, or non-pathogenic fungi, which can result in functional responses in receptor- or non-receptor-mediated fashions. endogenous ligands in the fungi have been demonstrated to be important for mating in a number of systems. mammalian hormones have been demonstrated to have stimulatory or inhibitory effects on g ... | 2016 | 26589227 |
| experimental protocol for the recovery and evaluation of bioactive compounds of tarbush against postharvest fruit fungi. | the aim of this study was to recover and evaluate in vitro the antifungal activity of bioactive compounds of tarbush flourensia cernua against fruit postharvest fungi and their antioxidant capacity. a yield of 15% of bioactive compounds of tarbush was obtained by infusion method and heating using water as solvent. a concentration of 4000 mg/l showed a higher antioxidant activity against the abts radical (3.21 μmol/g) in comparison with the dpph radical (7.62 μmol/g); however the dpph radical sho ... | 2016 | 26769505 |
| characterization of α-amylase from soursop (annona muricata linn.) fruits degraded by rhizopus stolonifer. | rhizopus stolonifer is a fungus and one of the most common species of the genus rhizopus. the organism has been a very important microbe used in the field of industrial microbiology. it has been used in the production of many hydrolytic and extracellular enzymes among which is the α-amylase. this enzyme has found various uses in the industry. fruit juices are important sources of nutrients and they contain several important therapeutic properties that may reduce the risk of various diseases. an ... | 2016 | 29023043 |
| reduction of chemical and biological oxygen demands from oil wastes via oleaginous fungi: an attempt to convert food by products to essential fatty acids. | the production of waste pollutants has become a major problem for many food and oil industries. however, oil wastes can provide alternative substrates for industry, which could help to solve environmental pollution problems. furthermore, oil wastes can be used as substrates to produce unsaturated fatty acids, which are important for health. | 2015 | 28959287 |
| growth simulation and discrimination of botrytis cinerea, rhizopus stolonifer and colletotrichum acutatum using hyperspectral reflectance imaging. | this research aimed to develop a rapid and nondestructive method to model the growth and discrimination of spoilage fungi, like botrytis cinerea, rhizopus stolonifer and colletotrichum acutatum, based on hyperspectral imaging system (his). a hyperspectral imaging system was used to measure the spectral response of fungi inoculated on potato dextrose agar plates and stored at 28°c and 85% rh. the fungi were analyzed every 12 h over two days during growth, and optimal simulation models were built ... | 2015 | 26642054 |
| effect of tea tree (melaleuca alternifolia) oil as a natural antimicrobial agent in lipophilic formulations. | there has been increased interest surrounding the use of tea tree oil (tto) as a natural antimicrobial. in this study, the antimicrobial activity of tto and its components were investigated in vitro and in a predominantly lipid-based personal care formulation. in vitro, tto showed minimal inhibitory concentrations of 0.2% (for saccharomyces cerevisiae and pythium sulcatum), 0.4% (for escherichia coli, bacillus subtilis, and rhizopus stolonifer), and 0.8% (for botrytis cinerea). tto at 0.08%-0.8% ... | 2015 | 25515896 |
| effects of ionizing radiation on postharvest fungal pathogens. | postharvest diseases cause losses in a wide variety of crops around the world. irradiation, a useful nonchemical approach, has been used as an alternative treatment for fungicide to control plant fungal pathogens. for a preliminary study, ionizing radiations (gamma, x-ray, or e-beam irradiation) were evaluated for their antifungal activity against botrytis cinerea, penicillium expansum, and rhizopus stolonifer through mycelial growth, spore germination, and morphological analysis under various c ... | 2015 | 26060436 |
| effect of linker flexibility on the catalytic features of a glycoside hydrolase family 45 endoglucanase from rhizopus stolonifer. | this study investigated an endoglucanase (egii) from rhizopus stolonifer var. reflexus tp-02 that consists of a c-terminal catalytic domain and an n-terminal carbohydrate-binding module joined by a linker rich in glycine, serine, threonine, and alanine. site-directed mutagenesis was applied to characterize the conformation and dynamics of the linker. mutants were expressed in escherichia coli bl21 and purified by ni-chelating column. structural analysis indicated that glycine provided flexibilit ... | 2015 | 26210786 |
| characterization of films based on chitosan lactate and its blends with oxidized starch and gelatin. | minimal inhibitory concentration (mic) of chitosan lactate (chl) was tested against bacteria and phytopathogenic fungi. then, the structural, physicochemical and antimicrobial properties of films based on chl, oxidized potato starch (ops), and gelatin (gel) were investigated. with the exception of rhizopus nigricans, chl was effective against the target organisms. gram-positive bacteria (staphylococcus aureus and bacillus cereus) were more sensitive to chl than gram-negative bacteria (pectobacte ... | 2015 | 25841370 |
| coatings comprising chitosan and mentha piperita l. or mentha × villosa huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit. | in this study, we evaluated the efficacy of coatings comprising shrimp chitosan (chi) and mentha piperita l. (mpeo) or mentha × villosa huds (mveo) essential oils to control mold infections caused by aspergillus niger, botrytis cinerea, penicillium expansum and rhizopus stolonifer in cherry tomato fruits (solanum lycopersicum l.) during storage at room temperature (25°c for 12 days) and low temperature (12°c for 24 days). the effects of the coatings on the physicochemical and sensory characteris ... | 2015 | 26313246 |
| fermentation optimization and unstructured kinetic model for cellulase production by rhizopus stolonifer var. reflexus tp-02 on agriculture by-products. | agricultural by-products, rice straw, wheat bran juice, and soybean residue, were used as substrates for cellulase production using rhizopus stolonifer var. reflexus tp-02. the culture medium was optimized though uniform design experimentation during shaking flask fermentation, and the ideal formulation obtained for filter paper enzyme (fpase) production was 10 % bran diffusion juice, 1 % rice straw, 0.17 % urea, 0.17 % soybean residue, 0.11 % kh2po4, and 0.027 % tween 80, and the maximal fpase ... | 2015 | 26400494 |
| mycelial mattress from a sporangia formation-delayed mutant of rhizopus stolonifer as wound healing-enhancing biomaterial. | a mycelial mattress of rhizopus stolonifer obtained from a liquid static culture was utilized for wound dressing and biomedical use. following screening of mutants induced by uv radiation, f6, exhibiting delayed sporangium formation was selected because its sporangium maturation exhibited a 5-day delay without significant loss of mycelial weight compared to the wild type. the sporangium-free mycelial mattress from the sporangiospore culture of f6 was treated with 1n sodium hydroxide naoh at 85°c ... | 2015 | 26275241 |
| in vitro antifungal activity and mechanism of action of tea polyphenols and tea saponin against rhizopus stolonifer. | the in vitro antifungal activities and mechanism of action of tea polyphenols (tp), tea saponin (ts) and their combination were evaluated against rhizopus stolonifer. the results showed that both tp and ts inhibited the mycelial growth in a dose-dependent manner, and their combination at the ratio of 7:3 exhibited synergistic antifungal interaction. we also observed that the treatment of tp or ts significantly induced the production of h2o2 and resulted in membrane lipid peroxidation, thus leadi ... | 2015 | 26138353 |
| antifungal activity and mechanism of tea polyphenols against rhizopus stolonifer. | to investigate the antifungal activity and possible mechanism of tea polyphenols (tps) against rhizopus stolonifer, the agent of rotting in nectarines and peaches. | 2015 | 26003094 |
| toxic ipomeamarone accumulation in healthy parts of sweetpotato (ipomoea batatas l. lam) storage roots upon infection by rhizopus stolonifer. | furanoterpenoid accumulation in response to microbial attack in rotting sweetpotatoes has long been linked to deaths and lung edema of cattle in the world. however, it is not known whether furanoterpenoid ipomeamarone accumulates in the healthy-looking parts of infected sweetpotato storage roots. this is critical for effective utilization as animal feed and assessment of the potential negative impact on human health. therefore, we first identified the fungus from infected sweetpotatoes as a rhiz ... | 2015 | 25418792 |
| in vitro antimalarial studies of novel artemisinin biotransformed products and its derivatives. | biotransformation of antimalarial drug artemisinin by fungi rhizopus stolonifer afforded three sesquiterpenoid derivatives. the transformed products were 1α-hydroxyartemisinin (3), 3.0%, a new compound, 10β-hydroxyartemisinin, 54.5% (4) and deoxyartemisinin (2) in 9% yield. the fungus expressed high-metabolism activity (66.5%). the chemical structures of the compounds were elucidated by 1d, 2d nmr spectrometry and mass spectral data. the major compound 10β-hydroxyartemisinin (4) was chemically c ... | 2014 | 25183484 |
| effects of chitosan and oligochitosan on development and mitochondrial function of rhizopus stolonifer. | the antifungal activities of chitosan and oligochitosan have been used to control postharvest decay of the fruits. the effect of chitosan and oligochitosan on mycelium growth, spore germination, and mitochondrial function of rhizopus stolonifer was evaluated in order to establish a connection between fungus development and the main organelle in charge to provide energy to the cell. the mycelium growth of r. stolonifer was significantly reduced on minimum media amended with chitosan or oligochito ... | 2014 | 24771597 |
| antifungal activity of plant extracts against aspergillus niger and rhizopus stolonifer. | despite recent advances in antifungal development, fungi remain a devastating threat to human health and compromise viability of the food supply. plant based antimicrobials represent a vast untapped source with tremendous potential. herein we present the antifungal properties of more than 50 plant extracts against two important human and agricultural pathogens, aspergillus niger and rhizopus stolonifer. multiple extracts exhibit promising mic values of less than 100 μg/ml and are reported for bo ... | 2014 | 25532291 |
| effects of post-harvest treatment using chitosan from mucor circinelloides on fungal pathogenicity and quality of table grapes during storage. | the aim of this study was to extract chitosan (chi) from mucor circinelloides ucp 050 grown in a corn steep liquor (csl)-based medium under optimized conditions and to assess the efficacy of the obtained chi to inhibit the post-harvest pathogenic fungi aspergillus niger urm 5162 and rhizopus stolonifer urm 3482 in laboratory media and as a coating on table grapes (vitis labrusca l.). the effect of chi coating on some physical, physicochemical and sensory characteristics of the fruits during stor ... | 2014 | 25084665 |
| synthesis of mannich bases: 2-(3-phenylaminopropionyloxy)-benzoic acid and 3-phenylamino-1-(2,4,6-trimethoxy-phenyl)-propan-1-one, their toxicity, ionization constant, antimicrobial and antioxidant activities. | mannich bases 2-(3-phenylaminopropionyloxy)-benzoic acid (a) and 3-phenylamino-1-(2,4,6-trimethoxy-phenyl)-propan-1-one (b) were synthesized. structures were confirmed by ultraviolet/visible and infra-red spectroscopies. the ionization constant (pka) values at 8.3 and 8.0 reported for compounds a and b, respectively, indicated that protonation might occur at physiological ph. the lc50 values of 145,595 μg/ml (a) and 82,526 μg/ml (b) obtained from brine shrimp lethality testing showed that both c ... | 2014 | 25038706 |
| antifungal effect of antimicrobial peptides (amps lr14) derived from lactobacillus plantarum strain lr/14 and their applications in prevention of grain spoilage. | the concern for food safety has led to an increased interest in the development of novel antimicrobials. keeping this aim in mind, we have investigated the antifungal effect of antimicrobial peptides (amps lr14) produced by lactobacillus plantarum strain lr/14 against four spoilage fungi, namely, aspergillus niger, rhizopus stolonifer, mucor racemosus and penicillium chrysogenum. interestingly, all the four fungi were inhibited, suggesting that amps lr14 exhibited anti-fungal property. the pepti ... | 2014 | 24929709 |
| fungal mediated generation of mammalian metabolites of fenofibrate and enhanced pharmacological activity of the main metabolite fenofibric acid. | different fungi viz. aspergillus niger ncim 589, a.ochraceous ncim 1140, cunninghamella blakesleeana ncim 687, c. echinulata ncim 691, rhizopus stolonifer ncim 880, mucor rouxi mtcc 386, trichothecium roseum ncim 1147 were screened for their potential to biotransform anti-hyperlipidemia and anti-hypertriglyceridemia drug, fenofibrate to fenofibric acid, the active metabolite and other mammalian metabolites. among the fungi screened c. blakesleeana transformed fenofibrate to fenofibric acid and o ... | 2014 | 24910236 |
| optimization of xylanase production by filamentous fungi in solid-state fermentation and scale-up to horizontal tube bioreactor. | five microorganisms, namely aspergillus niger cect 2700, a. niger cect 2915, a. niger cect 2088, aspergillus terreus cect 2808, and rhizopus stolonifer cect 2344, were grown on corncob to produce cell wall polysaccharide-degrading enzymes, mainly xylanases, by solid-state fermentation (ssf). a. niger cect 2700 produced the highest amount of xylanases of 504 ± 7 u/g dry corncob (dcc) after 3 days of fermentation. the optimization of the culture broth (5.0 g/l nano3, 1.3 g/l (nh4)2so4, 4.5 g/l kh2 ... | 2014 | 24728763 |
| assessment of allergenicity to fungal allergens of rohtak city, haryana, india. | fungal spores are known as one of the important bioparticles causing allergic manifestation in human beings. hence, knowledge of season and prevalence of the airborne allergens to which the patients are exposed is a prerequisite for proper diagnosis and treatment of allergic disorders in hypersensitive individuals. keeping this in view, aerial survey was performed in the atmosphere of rohtak city for 2 consecutive years (march 2008-february 2010), using a volumetric petri plate sampler. a total ... | 2014 | 24988378 |
| expression and functional analysis of a glycoside hydrolase family 45 endoglucanase from rhizopus stolonifer. | a novel endoglucanase gene was cloned from rhizopus stolonifer and expressed in escherichia coli, the gene product eg ii (45 kda) was assigned to glycoside hydrolase family 45 (gh45), and its specific activity on phosphoric acid-swollen cellulose (pasc) was 48 iu/mg. to solve the problem of substrate accumulation in the cellulose hydrolysis and enhance the catalytic efficiency of endoglucanase, the eg2 gene was modified by site directed mutagenesis. mutations generated by overlapping pcr have be ... | 2014 | 25164957 |
| stereo and regioselective microbial reduction of the clerodane diterpene 3,12-dioxo-15,16-epoxy-4-hydroxycleroda-13(16),14-diene. | the biotransformation of the clerodane diterpene, 3,12-dioxo-15,16-epoxy-4-hydroxy-cleroda-13(16),14-diene (1), obtained from croton micans var. argyroglossum (baill.) mill., was investigated for the first time. whole cells of cunninghamella echinulata and rhizopus stolonifer were used as enzymatic systems, and with both fungi the only biotransformation product obtained was the new ent-neo-clerodane diterpene (3r,4s,5s,8s,9r,10s)-3,4-dihydroxy-15,16-epoxy-12-oxo-cleroda-13(16),14-diene (2a). the ... | 2014 | 25115072 |
| interaction of antimicrobial cyclic lipopeptides from bacillus subtilis influences their effect on spore germination and membrane permeability in fungal plant pathogens. | bacillus subtilis cyclic lipopeptides are known to have various antimicrobial effects including different types of interactions with the cell membranes of plant pathogenic fungi. the various spectra of activities of the three main lipopeptide families (fengycins, iturins, and surfactins) seem to be linked to their respective mechanisms of action on the fungal biomembrane. few studies have shown the combined effect of more than one family of lipopeptides on fungal plant pathogens. in an effort to ... | 2014 | 25442289 |
| effects of fengycin from bacillus subtilis fmbj on apoptosis and necrosis in rhizopus stolonifer. | the lipopeptide antibiotic fengycin, produced by bacillus subtilis, strongly inhibits growth of filamentous fungi. in this study, we evaluated the effects of fengycin treatment on apoptosis and necrosis in rhizopus stolonifer by means of cell staining and epifluorescence microscopy. at fengycin concentrations less than 50 μg/ml, treated fungal cells demonstrated a dose-dependent increase in apoptosis-associated markers compared with the untreated control. these markers included chromatin condens ... | 2014 | 25098563 |
| regulation of the rana sylvatica brevinin-1sy antimicrobial peptide during development and in dorsal and ventral skin in response to freezing, anoxia and dehydration. | brevinin-1sy is the only described antimicrobial peptide (amp) of rana sylvatica. as amps are important innate immune molecules that inhibit microbes, this study examined brevinin-1sy regulation during development and in adult frogs in response to environmental stress. the brevinin-1sy nucleotide sequence was identified and used for protein modeling. brevinin-1sy was predicted to be an amphipathic, hydrophobic, alpha helical peptide that inserts into a lipid bilayer. brevinin-1sy transcripts wer ... | 2014 | 24436376 |
| fungal lectins: a growing family. | fungi are members of a large group of eukaryotic organisms that include yeasts and molds, as well as the most familiar member, mushrooms. fungal lectins with unique specificity and structures have been discovered. in general, fungal lectins are classified into specific families based on their amino acid sequences and three-dimensional structures. in this chapter, we provide an overview of the approximately 80 types of mushroom and fungal lectins that have been isolated and studied to date. in pa ... | 2014 | 25117221 |
| unraveling the protein network of tomato fruit in response to necrotrophic phytopathogenic rhizopus nigricans. | plants are endowed with a sophisticated defense mechanism that gives signals to plant cells about the immediate danger from surroundings and protects them from pathogen invasion. in the search for the particular proteins involved in fruit defense responses, we report here a comparative analysis of tomato fruit (solanum lycopersicum cv. ailsa craig) infected by rhizopus nigricans ehrenb, which is a significant contributor to postharvest rot disease in fresh tomato fruits. in total, four hundred f ... | 2013 | 24023804 |
| investigating the efficacy of bacillus subtilis sm21 on controlling rhizopus rot in peach fruit. | the efficacy of bacillus subtilis sm21 on controlling rhizopus rot caused by rhizopus stolonifer in postharvest peach fruit and the possible mechanisms were investigated. the results indicated b. subtilis sm21 treatment reduced lesion diameter and disease incidence by 37.2% and 26.7% on the 2nd day of inoculation compared with the control. the in vitro test showed significant inhibitory effect of b. subtilis sm21 on mycelial growth of r. stolonifer with an inhibition rate of 48.9%. b. subtilis s ... | 2013 | 23673059 |
| heterologous expression and secretion of an antifungal bacillus subtilis chitosanase (csnv26) in escherichia coli. | the aims of the study were the production improvement, the purification, the characterization and the activity investigation of chitosanase csnv26 of bacillus subtilis (v26). the gene csnv26 encoding for this protein was amplified and cloned in the pbad vector then expressed in escherichia coli (top10). the sds-page and zymogram analysis of the recombinant protein showed that it has two active forms sized 27 and 31 kda, corresponding to the protein with and without signal peptide. this protein h ... | 2013 | 23065029 |
| transcriptomic and morphological profiling of aspergillus fumigatus af293 in response to antifungal activity produced by lactobacillus plantarum 16. | the morphological effects of an antifungal activity produced by lactobacillus plantarum 16, a malt-production steep water isolate, on two food-associated fungi were examined microscopically. spore germination was completely inhibited in aspergillus fumigatus and rhizopus stolonifer upon treatment with concentrated cell-free supernatant (ccfs) of strain 16. furthermore, addition of antifungal ccfs to germ tubes and hyphae halted further development compared to untreated controls. transcriptome an ... | 2013 | 23876797 |
| broad-spectrum antifungal-producing lactic acid bacteria and their application in fruit models. | a large-scale screen of some 7,000 presumptive lactic acid bacteria (lab), isolated from animal, human, or plant origin, identified 1,149 isolates with inhibitory activity against the food-spoilage mould penicillium expansum. in excess of 500 lab isolates were subsequently identified to produce a broad spectrum of activity against p. expansum, penicillium digitatum, penicillium notatum, penicillium roqueforti, rhizopus stolonifer, fusarium culmorum, aspergillus fumigatus and rhodotorula mucilagi ... | 2013 | 23160868 |
| biocontrol of postharvest rhizopus decay of peaches with pichia caribbica. | a new yeast antagonist, pichia caribbica, isolated in our laboratory from the soil collected from unsprayed orchards, was evaluated for its biocontrol capability against rhizopus stolonifer on peaches and the possible mechanisms involved. the decay incidence and lesion diameter of rhizopus decay of peaches treated by p. caribbica were significantly reduced compared with the control fruits, and the higher the concentration of p. caribbica, the better the efficacy of the biocontrol. rapid coloniza ... | 2013 | 23536217 |
| bacillus cereus ar156 induces resistance against rhizopus rot through priming of defense responses in peach fruit. | the biocontrol effects of bacillus cereus ar156 on rhizopus rot caused by rhizopus stolonifer in postharvest peach fruit and the possible mechanisms were investigated. the results showed that fruit treated with b. cereus ar156 had significantly lower disease incidence and smaller lesion diameter than the control fruit did. b. cereus ar156 treatment remarkably enhanced activities of chitinase and β-1,3-glucanase, promoted accumulation of h(2)o(2), and improved total phenolic content and 2,2-diphe ... | 2013 | 23122077 |
| antifungal metabolites produced by chaetomium globosum no.04, an endophytic fungus isolated from ginkgo biloba. | the fungal endophyte chaetomium globosum no.04 was isolated from the medicinal plant ginkgo biloba. the crude extract of the fungus fermentation were active in the agar-diffusion tests against the phytopathogenic fungi rhizopus stolonifer and coniothyrium diplodiella. further bioassay-guided chemical investigation led to the isolation and purification of six alkaloids and three non-targeted compounds from 50 l fermentation of this endophytic fungus and their structures were elucidated as chaetog ... | 2013 | 24426105 |
| in vitro antifungal activity of three geophytic plant extracts against three post-harvest pathogenic fungi. | plant extracts appear to be one of the most effective alternative methods of plant diseases control which are less harmful to human beings and environment. in vitro antifungal activity of methanolic extracts of three promising wild geophytic plants against three post-harvest pathogenic fungi using radial growth technique was conducted. these extracts included the shoot system (s) and underground parts (r) of asparagus stipularis, cyperus capitatus and stipagrostis lanata. the tested fungi were a ... | 2013 | 24506036 |
| liposome-mediated mycelial transformation of filamentous fungi. | liposome-mediated transformation is common for cells with no cell wall, but has very limited usage in cells with walls, such as bacteria, fungi, and plants. in this study, we developed a procedure to introduce dna into mycelium of filamentous fungi, rhizopus nigricans lh 21 and pleurotus ostreatus td 300, by liposome-mediation but with no protoplast preparation. the dna was transformed into r. nigricans via plasmid pegfp-c1 and into p. ostreatus via 7.2 kb linear dna. the mycelia were ground in ... | 2013 | 24012298 |
| polysaccharides from rhizopus nigricans mycelia induced apoptosis and g2/m arrest in bgc-823 cells. | in this study, we reported that polysaccharides (rps) from the mycelia of liquid-cultured rhizopus nigricans inhibited the proliferation and clonogenic potential of human gastric cancer bgc-823 cells. results of acridine orange/ethidium bromide and hoechst 33258 staining showed that treated cells displayed typical morphological characteristics of apoptosis such as condensation of chromatin, nuclear pyknosis and formation of apoptotic bodies. flow cytometry analyses and colorimetric assay indicat ... | 2013 | 23911518 |
| the thermostability of two kinds of recombinant ∆6-fatty acid desaturase with different n-terminal sequence lengths in low temperature. | two recombinant rhizopus stolonifer ∆6-fatty acid desaturase enzymes with different-length n-termini were cloned and expressed in saccharomyces cerevisiae strain invscl: lrsd6d begins with the sequence of the n-terminal of the r. stolonifer ∆6-fatty acid desaturase native, encoding a deduced polypeptide of 459 amino acids (m-s-t-l-d-r-q-s-i-f-t-i-k-e-l-e-s-i-s-q-r-i-h-d-g-d-e-e-a-m-k-f), whereas srsd6d begins with the amino acid sequence of the predicted orf, encoding a deduced polypeptide of 43 ... | 2013 | 23821293 |
| biotransformation of clerodane diterpenoids by rhizopus stolonifer and antibacterial activity of resulting metabolites. | microbial transformation of clerodane lactone (1) by a plant pathogen fungus, rhizopus stolonifer, resulted in the production of metabolites 3 and 4. while incubation of clerodane methyl ester (2) by r. stolonifer yielded metabolites 5-8. the structures of the transformed products were determined by the spectroscopic techniques and compounds 4, 7 and 8 were found. the antibacterial activity of clerodane diterpenoids 1 and 2 and their metabolites 3-8 were also studied. the metabolites 3-7 showed ... | 2013 | 23535269 |
| degradation of metalaxyl and folpet by filamentous fungi isolated from portuguese (alentejo) vineyard soils. | degradation of xenobiotics by microbial populations is a potential method to enhance the effectiveness of ex situ or in situ bioremediation. the purpose of this study was to evaluate the impact of repeated metalaxyl and folpet treatments on soil microbial communities and to select soil fungal strains able to degrade these fungicides. results showed enhanced degradation of metalaxyl and folpet in vineyards soils submitted to repeated treatments with these fungicides. indeed, the greatest degradat ... | 2013 | 23430293 |
| production, statistical optimization and application of endoglucanase from rhizopus stolonifer utilizing coffee husk. | coffee cherry husk (ch) is one of the major by-products obtained from coffee processing industry and accounts to 43 ± 5.9% of cellulose. screening of fungal organism for cellulase production was carried out and the potential organism was identified as rhizopus stolonifer by internal transcribed spacer's (its)-5.8s rdna analysis. a systematic study with response surface methodology (rsm) based on ccrd was used to study the interactions among the variables such as ph (3-7), moisture (40-80%) and p ... | 2013 | 23223909 |
| the mitochondrial respiratory chain of rhizopus stolonifer (ehrenb.:fr.) vuill. | rhizopus stolonifer (ehrenb.:fr.) vuill mitochondria contain the complete system for oxidative phosphorylation, formed by the classical components of the electron transport chain (complexes i, ii, iii, and iv) and the f(1)f(0)-atp synthase (complex v). using the native gel electrophoresis, we have shown the existence of supramolecular associations of the respiratory complexes. the composition and stoichiometry of the oxidative phosphorylation complexes were similar to those found in other organi ... | 2013 | 23064442 |
| [harmful biological agents at museum workposts]. | the aim of the studies was to determine the level and kind of microbiological contamination of air and surfaces in museum premises with various collection specificities. in addition, the criteria for selecting indicators of contamination with harmful biological agents at museum workposts are proposed. | 2012 | 22779322 |
| antioxidant, antifungal and antiviral activities of chitosan from the larvae of housefly, musca domestica l. | antioxidant activity of the chitosan from the larvae of musca domestica l. was evaluated in two different reactive oxygen species assays, and inhibitory effects against seven fungi were also tested. the results showed that the chitosan had scavenging activity for hydroxyl and superoxide radicals which were similar to that of ascorbic acid. also the chitosan exhibited excellent antifungal activity, especially in the low concentration, it could significantly inhibit the growth of rhizopus stolonif ... | 2012 | 26434321 |
| time-course changes in fungal elicitor-induced lignan synthesis and expression of the relevant genes in cell cultures of linum album. | linum album has been shown to accumulate anti-tumor podophyllotoxin (ptox) and its related lignans. in the present study, we examined the effects of five fungal extracts on the production of lignans in l. album cell cultures. fusarium graminearum extract induced the highest increase of ptox [140μgg(-1) dry weight (dw) of the l. album cell culture] which is seven-fold greater than the untreated control, while rhizopus stolonifer extract enhanced the accumulation of lariciresinol, instead of ptox, ... | 2012 | 22218086 |
| synthesis of some potent immunomodulatory and anti-inflammatory metabolites by fungal transformation of anabolic steroid oxymetholone. | biotransformation of organic compounds by using microbial whole cells provides an efficient approach to obtain novel analogues which are often difficult to synthesize chemically. in this manuscript, we report for the first time the microbial transformation of a synthetic anabolic steroidal drug, oxymetholone, by fungal cell cultures. | 2012 | 23237028 |
| efficacy of the application of a coating composed of chitosan and origanum vulgare l. essential oil to control rhizopus stolonifer and aspergillus niger in grapes (vitis labrusca l.). | this study evaluated the efficacy of the combined application of chitosan (chi) and origanum vulgare l. essential oil (ov) in the inhibition of rhizopus stolonifer urm 3728 and aspergillus niger urm 5842 on laboratory media and on grapes (vitis labrusca l.) and its influence on the physical, physicochemical and sensory characteristics of the fruits during storage (25 °c, 12 days and 12 °c, 24 days). the application of mixtures of different chi and ov concentrations (minimum inhibitory concentrat ... | 2012 | 22986200 |
| generation of the volatile spiroketals conophthorin and chalcogran by fungal spores on polyunsaturated fatty acids common to almonds and pistachios. | the spiroketal (e)-conophthorin has recently been reported as a semiochemical of the navel orangeworm moth, a major insect pest of california pistachios and almonds. conophthorin and the isomeric spiroketal chalcogran are most commonly known as semiochemicals of several scolytid beetles. conophthorin is both an insect- and plant-produced semiochemical widely recognized as a nonhost plant volatile from the bark of several angiosperm species. chalcogran is the principal aggregation pheromone compo ... | 2012 | 23153034 |
| mycobiota and mycotoxins (aflatoxins and ochratoxin) associated with some saudi date palm fruits. | this study aimed to determine the mycological profile of the retail date fruits distributed in different markets at taif, saudi arabia. the presence of aflatoxins and ochratoxin a was also measured. twenty-two fungal species belonging to 12 genera were isolated from 50 different date samples. aspergillus flavus, a. niger, penicillium chrysogenum, and rhizopus stolonifer were the most prevalent species among isolated fungi. eighty isolates of a. flavus and 36 of a. niger were detected for their a ... | 2012 | 22568750 |
| novel copper-based therapeutic agent for anti-inflammatory: synthesis, characterization, and biochemical activities of copper(ii) complexes of hydroxyflavone schiff bases. | four hydroxyflavone derivatives have been synthesized with the aim of obtaining a good model of superoxide dismutase. better to mimic the natural metalloenzyme, copper complexes have been designed. the cu(ii) complexes of general formulae, [cul] where l = 5-hydroxyflavone-o-phenylenediamine (l¹h₂)/m-phenylenediamine (l²h₂) and 3-hydroxyflavone-o-phenylenediamine (l³h₂)/m-phenylenediamine (l⁴h₂) have been synthesized. the structural features have been determined from their analytical and spectral ... | 2012 | 22238015 |
| occupational exposure to airborne fungi in two croatian sawmills and atopy in exposed workers. | airborne fungi were collected over a one year period at 2-month intervals at 2 sawmills in croatia (sm 1 and sm 2) processing mainly beech wood and oak wood. a questionnaire concerning respiratory symptoms and skin prick test (spt) with common inhalatory allergens and moulds cladosporium herbarum, alternaria alternata, aspergillus niger, penicillium notatum, and rhizopus nigricans were performed in 96 workers from the same sawmills. average concentrations of airborne fungi were 1,696-7,316 cfu/m ... | 2012 | 22742790 |
| growth rate inhibition of phytopathogenic fungi by characterized chitosans. | the inhibitory effects of fifteen chitosans with different degrees of polymerization (dp) and different degrees of acetylation (fa) on the growth rates (gr) of four phytopathogenic fungi (alternaria alternata, botrytis cinerea, penicillium expansum, and rhizopus stolonifer) were examined using a 96-well microtiter plate and a microplate reader. the minimum inhibitory concentrations (mics) of the chitosans ranged from 100 μg ×ml(-1) to 1,000 μg ×ml(-1) depending on the fungus tested and the dp an ... | 2012 | 24031893 |
| antifungal and antioxidative potential of oil and extracts derived from leaves of indian spice plant cinnamomum tamala. | plant—based antimicrobials and antioxidants represent a vast untapped source for medicines and food supplements and hence have enormous therapeutic potential. present work reports the fungicidal potential of cinnamomum tamala nees & eberm (lauraceae) leaf oil against five food spoilage and pathogenic fungi. in addition antioxidant efficacy of seven different solvent extracts derived from leaf was also evaluated using in vitro models. the oil demonstrated potent antifungal activity against asperg ... | 2012 | 23273204 |
| A new antifungal coumarin from Clausena excavata. | A new ?-lactone coumarin, named as excavarin-A, showing antifungal activity was isolated from the leaves of Clausena excavata by bioassay guided fractionation method. The structure was elucidated by spectroscopic data analysis and identified as 7((2E)-4(4,5-dihydro-3-methylene-2-oxo-5-furanyl)-3-methylbut-2-enyloxy) coumarin. Minimum inhibitory concentration (MIC) was determined against fifteen fungal strains pathogenic against plants and human. The least MIC was recorded against the human patho ... | 2012 | 22088496 |
| in vitro control of post-harvest fruit rot fungi by some plant essential oil components. | eight substances that are main components of the essential oils from three mediterranean aromatic plants (verbena officinalis, thymus vulgaris and origanum vulgare), previously found active against some phytopathogenic fungi and stramenopila, have been tested in vitro against five etiological agents of post-harvest fruit decay, botrytis cinerea, penicillium italicum, p. expansum, phytophthora citrophthora and rhizopus stolonifer. the tested compounds were β-fellandrene, β-pinene, camphene, carva ... | 2012 | 22408454 |
| zygomycota associated with traditional meju, a fermented soybean starting material for soy sauce and soybean paste. | various zygomycota species were detected during a study of the mycobiota of meju, a brick of dried fermented soybeans, used in korean cuisine. two hundred and sixty-eight strains were isolated from 98 finished meju products collected in various regions of korea from 2009 to 2011, and 96 strains were isolated from in-process meju on various farms from 2010 to 2011. the isolated zygomycota were identified using phenotypic characteristics combined with dna sequences of the internal transcribed spac ... | 2012 | 22752901 |
| antimicrobial activity of saponin-rich fraction from camellia oleifera cake and its effect on cell viability of mouse macrophage raw 264.7. | as a by-product of oil production, several million tons of camellia oleifera cake is discarded every year in china. the aim of this study was to evaluate the antimicrobial activity of a saponin-rich fraction isolated from c. oleifera cake and investigate its effect on the cell viability of raw 264.7, a macrophage-like cell line present in almost all mouse tissues. | 2012 | 22430639 |
| antifungal activity of essential oil from asteriscus graveolens against postharvest phytopathogenic fungi in apples. | the essential oils of the aerial parts of asteriscus graveolens have been studied using gc and gc-ms. twenty-eight compounds were identified in the essential oil amounting to 94.9% of the total oil. the aerial part oils showed similar chromatographic profiles and were characterized by having a high content of oxygenated sesquiterpenes with 6-oxocyclonerolidol (66.7% +/- 5.5) and 6-hydroxycyclonerolidol (8.8% +/-1.2) as the major components. the antifungal effect of the essential oil from a. grav ... | 2011 | 22224306 |
| antagonistic action of bacillus subtilis strain fmbj on the postharvest pathogen rhizopus stolonifer. | the antagonistic activities of bacillus subtilis fmbj against the rhizopus stolonifer pathogen causing peach soft rot disease were studied in this paper. bacillus subtilis fmbj exhibited a high antifungal effect on the mycelium growth, sportulation, and germ tube elongation of r. stolonifer with the inhibition rate of 86.5% and 97.42%, respectively, spore germination rate of 20% under antagonist strain concentration of 10⁸ cfu/ml. by using of scanning electron microscope (sem) and transmission e ... | 2011 | 22417435 |
| antifungal activity in ethanolic extracts of carica papaya l. cv. maradol leaves and seeds. | bioactive compounds from vegetal sources are a potential source of natural antifungic. an ethanol extraction was used to obtain bioactive compounds from carica papaya l. cv. maradol leaves and seeds of discarded ripe and unripe fruit. both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. the effect of time on extraction efficiency was confirmed by qualitative identif ... | 2011 | 22282629 |
| influence of fungal elicitation on glycyrrhizin production in transformed cell cultures of abrus precatorius linn. | glycyrrhizin, obtained from abrus precatorius (indian liquorice), is a phytoconstituent of importance for pharmaceutical and food industries. | 2011 | 22262933 |
| currency notes and coins as a possible source of transmitting fungal pathogens of man and plants. | currency (notes and coins) handling by people during transaction is one of the most mobile objects within the community, which has a potential of transmitting pathogens. a survey carried out recently in nanded city (maharashtra) revealed heavy contamination of currency notes and coins by important fungal pathogens of plants and man, i.e. aspergillus niger (60.37%), a. flavus (3.98%), a.nidulans (0.2%), penicillium citrinum (17.80%), alternaria tenuis (0.20%), curvularia pallescens (0.20%), clado ... | 2011 | 23505834 |
| interactions between various microbes and ginseng botanicals. | three kinds of interactions occur between ginseng botanicals and microorganisms: a) spoilage of the botanical by various fungi (e.g., aspergillus, penicillium, alternaria, and eurotium species) and bacteria; b) transformation of ginsenosides into more bioactive forms by bacteria such as intrasporangium sp. gs603, microbacterium sp. gs514, caulobacter leidyia, bifidobacterium sp. int57, bifidobacterium sp. sj32, fusobacterium sp. and bacteroides sp., and moulds (e.g., aspergillus niger, fusarium ... | 2011 | 21254831 |
| antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against rhizopus stolonifer. | the antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against rhizopus stolonifer were investigated. the mic (minimal inhibitory concentration) of fengycin without commercial surfactin added was 0.4 mg/ml while the mic of fengycin with commercial surfactin added was 2.0 mg/ml. fengycin acted on cell membrane and cellular organs and inhibited dna synthesis. the antifungal effect of fengycin was reduced after commercial surfactin was added. all these ... | 2011 | 21369992 |
| molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from rhizopus stolonifer strain yf6 which can accumulate high levels of gamma-linolenic acid. | the delta 6-desaturase gene was cloned from rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (gla, c18:3 ?(6,9,12)) to the total fatty acids. the cloned dna contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and hpgg motif. notably, this deduced sequence had a shorter c-terminus. results demonstrated that the cdna sequence exhibite ... | 2011 | 21369993 |
| natural resistance of rose petals to microbial attack. | petals of red, yellow and white roses (rosa damascene mill.) of the family rosaceae were extracted with (1:1) methylene chloride/methanol and tested for their antimicrobial activities against four species of gram-positive bacteria (bacillus cereus, bacillus subtilis, micrococcus luteus and staphylococcus aureus), five species of gram-negative bacteria (enterobacter aerogenes, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa and serratia marcescens) and five species of fungi (penici ... | 2011 | 21614712 |