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construction of a genome-anchored, high-density genetic map for melon (cucumis melo l.) and identification of fusarium oxysporum f. sp. melonis race 1 resistance qtl.four qtls and an epistatic interaction were associated with disease severity in response to inoculation with fusarium oxysporum f. sp. melonis race 1 in a recombinant inbred line population of melon. the usda cucumis melo inbred line, mr-1, harbors a wealth of alleles associated with resistance to several major diseases of melon, including powdery mildew, downy mildew, alternaria leaf blight, and fusarium wilt. mr-1 was crossed to an israeli cultivar, ananas yok'neam, which is susceptible to all ...201829372283
fruit flesh volatile and carotenoid profile analysis within the cucumis melo l. species reveals unexploited variability for future genetic breeding.aroma profile and carotenoids content of melon flesh are two important aspects influencing the quality of this fruit that have been characterized using only selected genotypes. however, the extant variability of the whole species remains unknown.201829369359
double function hydroperoxide lyases/epoxyalcohol synthases (cyp74c) of higher plants: identification and conversion into allene oxide synthases by site-directed mutagenesis.the cyp74c subfamily of fatty acid hydroperoxide transforming enzymes includes hydroperoxide lyases (hpls) and allene oxide synthases (aoss). this work reports a new facet of the putative cyp74c hpls. initially, we found that the recombinant cyp74c13_mt (medicago truncatula) behaved predominantly as the epoxyalcohol synthase (eas) towards the 9(s)-hydroperoxide of linoleic acid. at the same time, the cyp74c13_mt mostly possessed the hpl activity towards the 13(s)-hydroperoxides of linoleic and α ...201829325723
overexpression of the tonoplast sugar transporter cmtst2 in melon fruit increases sugar accumulation.fruits are an important part of the human diet and sugar content is a major criterion used to evaluate fruit quality. melon fruit accumulate high sugar concentrations during their development; however, the mechanism through which these sugars are transported into the vacuoles of fruit cells for storage remains unclear. in this study, three tonoplast sugar transporters (tsts), cmtst1, cmtst2, and cmtst3, were isolated from melon plants. analysis of subcellular localization revealed that all these ...201829309616
multitrait analysis of fresh-cut cantaloupe melon enables discrimination between storage times and temperatures and identifies potential markers for quality assessments.fresh-cut cantaloupe melon is valued for its aroma but is highly perishable. temperature of storage (typically 0-5°c) is critical for maintaining fresh-cut melon quality, but often reaches 10°c during transportation and in retail outlets. a comparison amongst 0, 5 and 10°c storage temperatures for fresh-cut melon over 14days reveals that storage at 0°c is optimal for avoiding increases in microbial load and loss of vitamin c especially at later time points. however, higher temperatures maintain ...201828958522
melonet-db, a grand rna-seq gene expression atlas in melon (cucumis melo l.).melon (cucumis melo l.) is an important cucurbitaceae crop produced worldwide, exhibiting wide genetic variations and comprising both climacteric and non-climacteric fruit types. the muskmelon cultivar "'earl's favorite harukei-3 (harukei-3)"' known for its sweetness and rich aroma is used for breeding of high-grade muskmelon in japan. we conducted rna sequencing (rna-seq) transcriptome studies in 30 different tissues of the 'harukei-3' melon. these included root, stems, leaves, flowers, regener ...201829216378
glabrous (cmgl) encodes a hd-zip iv transcription factor playing roles in multicellular trichome initiation in melon.map-based cloning identified cmgl that encodes a hd-zip type iv transcription factor that controls multicellular trichome initiation in melon. trichomes are small hairs covering the aerial parts of plants that originate from the epidermal cells, which can protect plants against the damage by insects and pathogens. the regulatory pathway of unicellular trichomes has been well studied in the model plant arabidopsis. little is known about the genetic control and regulation of trichome development i ...201729147724
volatile emerging contaminants in melon fruits, analysed by hs-spme-gc-ms.the aim of this research was to develop and validate a headspace-solid phase micro-extraction-gas chromatography-mass spectrometry (hs-spme-gc-ms) method for the determination of volatile emerging contaminants in fruit. the method showed good precision (rsd ≤ 14%) and satisfactory recoveries (99.1-101.7%) and lod and loq values ranging between 0.011-0.033 μg kg-1 and 0.037-0.098 μg kg-1, respectively. the method was applied to investigate the content of volatile emerging contaminants in two vari ...201729111874
hexanoic acid treatment prevents systemic mnsv movement in cucumis melo plants by priming callose deposition correlating sa and opda accumulation.unlike fungal and bacterial diseases, no direct method is available to control viral diseases. the use of resistance-inducing compounds can be an alternative strategy for plant viruses. here we studied the basal response of melon to melon necrotic spot virus (mnsv) and demonstrated the efficacy of hexanoic acid (hx) priming, which prevents the virus from systemically spreading. we analysed callose deposition and the hormonal profile and gene expression at the whole plant level. this allowed us t ...201729104580
antipyretic effects of hydro-methanol extract of melia azedarach linn. seeds and cucumis melo linn. seeds in experimental rabbits.to investigate the antipyretic activity of hydro-methanol extract of melia azedarach linn. (hmema) seeds and cucumis melo linn. (hmecm) seeds in experimental animals. baker's yeast was used to induce fever in rabbits which were divided into six groups. the animal groups were thereafter administered distilled water (control), paracetamol (reference standard, 150mg/kg), hmema (250mg/kg), hmema (500mg/kg), hmecm (250mg/kg) and hmecm (500mg/kg) respectively. hmema and hmecm were also phytochemically ...201729039323
qtl analyses in multiple populations employed for the fine mapping and identification of candidate genes at a locus affecting sugar accumulation in melon (cucumis melo l.).sugar content is the major determinant of both fruit quality and consumer acceptance in melon (cucumis melo l), and is a primary target for crop improvement. near-isogenic lines (nils) derived from the intraspecific cross between a "piel de sapo" (ps) type and the exotic cultivar "songwhan charmi" (sc), and several populations generated from the cross of ps × ames 24294 ("trigonus"), a wild melon, were used to identify qtl related to sugar and organic acid composition. seventy-eight qtl were det ...201729018473
a re-sequencing-based ultra-dense genetic map reveals a gummy stem blight resistance-associated gene in cucumis melo.the melon (cucumis melo) genome and genetic maps with hundreds to thousands of single nucleotide polymorphism markers were recently released. however, a high-resolution genetic map was lacking. gummy stem blight (gsb) is a destructive disease responsible for considerable economic losses during melon production. we herein describe the development of an ultra-dense genetic map consisting of 12,932 recombination bin markers covering 1,818 cm, with an average distance of 0.17 cm between adjacent tag ...201728985339
chromatographic determination, decline dynamic and risk assessment of sulfoxaflor in asian pear and oriental melon.the dissipation pattern of sulfoxaflor in asian pear cultivated in an open field conditions and in oriental melon grown under plastic house conditions was each studied in two different locations. residues in field-treated samples were determined using liquid chromatography coupled with an ultraviolet detector and confirmed by liquid chromatography-tandem mass spectrometry. a calibration curve for sulfoxaflor was linear over the concentration range 0.1-5.0 mg/l, with a coefficient of determinatio ...201728975659
validation of a landscape-based model for whitefly spread of the cucurbit yellow stunting disorder virus to fall melons.the cucurbit yellow stunting disorder virus (cysdv) transmitted by bemisia tabaci (gennadius) (hemiptera: aleyrodidae) has caused significant reductions in fall melon (cucumis melo l.) yields in yuma county, arizona. in a recent landscape-based study, we found evidence that cotton and spring melon fields increased abundance of b. tabaci and spread of cysdv infection in fall melon fields. here, we show that a statistical model derived from data collected in 2011-2012 and based on areas of cotton ...201728961816
genotypic differences in physiological and biochemical responses to salinity stress in melon (cucumis melo l.) plants: prospects for selection of salt tolerant landraces.melon (cucumis melo l.) is one of the most important horticultural crops in iran often cultivated in arid and semiarid regions of the country with salinity problems. the objective of this work was to better understand the mechanisms of physiological and biochemical responses to salinity stress of five iranian melon landraces "samsuri", "kashan", "khatouni", "suski-e-sabz", and "ghobadlu" from different geographical origins, and "galia" f1 cultivar. plants were grown under greenhouse conditions a ...201728938176
analysis of genetic variability of commercial melon cultivars using ssr molecular markers.taking into account the scenario of melon production (cucumis melo l.) in brazil, it is notable the importance of the culture in the fruit market. the preference of the national market is concentrated in the fruits of the yellow type. therefore, it is possible that the genetic base of the commercial cultivars is relatively narrow due to the loss of important genes in the breeding process, and it is possible to introgress external genes for increasing recombination possibilities that generate sou ...201728873211
genome-wide linkage-disequilibrium mapping to the candidate gene level in melon (cucumis melo).cucumis melo is highly diverse for fruit traits providing wide breeding and genetic research opportunities, including genome-wide association (gwa) analysis. we used a collection of 177 accessions representing the two c. melo subspecies and 11 horticultural groups for detailed characterization of fruit traits variation and evaluation of the potential of gwa for trait mapping in melon. through genotyping-by-sequencing, 23,931 informative snps were selected for genome-wide analyses. we found that ...201728852011
dissipation kinetics, pre-harvest residue limits, and hazard quotient assessments of pesticides flubendiamide and fluopicolide in korean melon (cucumis melo l. var. makuwa) grown under regulated conditions in plastic greenhouses.the dissipation kinetics, pre-harvest residue limits, and hazard quotient (hq) assessments of the pesticides flubendiamide and fluopicolide were conducted for korean melon (cucumis melo l. var. makuwa) cultivated at two different sites. a single extraction and cleanup procedure was carried out using acetone (partitioned with dichloromethane) and amino solid-phase extraction cartridges, respectively. residue analysis was performed by hplc with ultraviolet detection. both pesticides showed excelle ...201728799066
proteogenomics data for deciphering frankia coriariae interactions with root exudates from three host plants.frankia coriariae bmg5.1 cells were incubated with root exudates derived from compatible (coriaria myrtifolia), incompatible (alnus glutinosa) and non-actinorhizal (cucumis melo) host plants. bacteria cells and their exoproteomes were analyzed by high-throughput proteomics using a q-exactive hf high resolution tandem mass spectrometer incorporating an ultra-high-field orbitrap analyzer. ms/ms spectra were assigned with two protein sequence databases derived from the closely-related genomes from ...201728765834
genome-wide identification of hami melon mirnas with putative roles during fruit development.micrornas represent a family of small endogenous, non-coding rnas that play critical regulatory roles in plant growth, development, and environmental stress responses. hami melon is famous for its attractive flavor and excellent nutritional value, however, the mechanisms underlying the fruit development and ripening remains largely unknown. here, we performed small rna sequencing to investigate the roles of mirnas during hami melon fruit development. two batches of flesh samples were collected a ...201728742088
genome-wide analysis of simple sequence repeats in bitter gourd (momordica charantia).bitter gourd (momordica charantia) is widely cultivated as a vegetable and medicinal herb in many asian and african countries. after the sequencing of the cucumber (cucumis sativus), watermelon (citrullus lanatus), and melon (cucumis melo) genomes, bitter gourd became the fourth cucurbit species whose whole genome was sequenced. however, a comprehensive analysis of simple sequence repeats (ssrs) in bitter gourd, including a comparison with the three aforementioned cucurbit species has not yet be ...201728690629
mapping and characterization of the fefe gene that controls iron uptake in melon (cucumis melo l.).iron (fe) deficiency in plants limits crop growth and productivity. molecular mechanisms that plants use to sense and respond to fe deficiency by coordinated expression of fe-uptake genes are not fully understood. the c940-fe chlorotic melon (cucumis melo) mutant known as fefe is unable to upregulate fe-uptake genes, however, the fefe gene had not been identified. in this study, we used two f2 mapping populations to map and identify the fefe gene as bhlh38, a homolog of subgroup ib bhlh genes fr ...201728659950
mapping and preliminary analysis of aborted microspores (ams) as the candidate gene underlying the male sterility (ms-5) mutant in melon (cucumis melo l.).melon is an important agricultural and economic vegetable crop worldwide. the genetic male sterility mutant (ms-5) has a recessive nuclear gene that controls the male sterility germplasm. male sterility could reduce the cost of f1 seed production in melon, but heterozygous fertile plants should be removed before pollination. in this study, bulked segregant analysis combined with specific length amplified fragment sequencing was applied to map the single nuclear male sterility recessive gene. a 3 ...201728611814
the quest for epigenetic regulation underlying unisexual flower development in cucumis melo.melon (cucumis melo) is an important vegetable crop from the cucurbitaceae family and a reference model specie for sex determination, fruit ripening and vascular fluxes studies. nevertheless, the nature and role of its epigenome in gene expression regulation and more specifically in sex determination remains largely unknown.201728592995
influence of environmental factors on cucumis melo l. var. agrestis naud. seed germination and seedling emergence.cucumis melo l. var. agrestis naud. (field muskmelon) is an annual invasive weed in many parts of asia. however, there is very little available information about the germination and emergence of this species. therefore, laboratory experiments were conducted to evaluate the effects of light, temperature, salt stress, osmotic stress, ph, and depth of planting on field muskmelon germination and seedling emergence. light had no effect on seed germination, and the seeds germinated at a wide range of ...201728570590
dissipation pattern and risk quotients assessment of amisulbrom in korean melon cultivated in plastic house conditions.amisulbrom formulated as suspension concentrate was applied at the rate recommended for korean melon to determine the dissipation pattern (at two different sites), the pre-harvest residue limit (phrl), and risk assessments. samples collected over 10 days were extracted using liquid-liquid extraction (lle) and cleaned up with solid-phase extraction (spe) florisil cartridge. residual concentrations were determined using liquid chromatography-ultraviolet detector (lc-uvd) and confirmed by liquid ch ...201728560618
green synthesis of highly stable carbon nanodots and their photocatalytic performance.the present study reports a novel, facile, biosynthesis route for the synthesis of carbon nanodots (cds) with an approximate quantum yield of 38.5%, using musk melon extract as a naturally derived-precursor material. the synthesis of cds was established by using ultraviolet-visible (uv-vis) spectroscopy, dynamic light scattering, photoluminescence spectroscopy, x-ray diffraction, transmission electron microscopy and fourier transform infrared (ftir) spectroscopy. the as-prepared cds possess an e ...201728530182
structure of eif4e in complex with an eif4g peptide supports a universal bipartite binding mode for protein translation.the association-dissociation of the cap-binding protein eukaryotic translation initiation factor 4e (eif4e) with eif4g is a key control step in eukaryotic translation. the paradigm on the eif4e-eif4g interaction states that eif4g binds to the dorsal surface of eif4e through a single canonical alpha-helical motif, while metazoan eif4e-binding proteins (m4e-bps) advantageously compete against eif4g via bimodal interactions involving this canonical motif and a second noncanonical motif of the eif4e ...201728522457
host plant compatibility shapes the proteogenome of frankia coriariae.molecular signaling networks in the actinorhizal rhizosphere select host-compatible frankia strains, trigger the infection process and eventually the genesis of nitrogen-fixing nodules. the molecular triggers involved remain difficult to ascertain. root exudates (re) are highly dynamic substrates that play key roles in establishing the rhizosphere microbiome. re are known to induce the secretion by rhizobia of nod factors, polysaccharides, and other proteins in the case of legume symbiosis. next ...201728512450
ethqv6.3 is involved in melon climacteric fruit ripening and is encoded by a nac domain transcription factor.fruit ripening is divided into climacteric and non-climacteric types depending on the presence or absence of a transient rise in respiration rate and the production of autocatalytic ethylene. melon is ideal for the study of fruit ripening, as both climacteric and non-climacteric varieties exist. two introgressions of the non-climacteric accession pi 161375, encompassed in the qtls ethqb3.5 and ethqv6.3, into the non-climacteric 'piel de sapo' background are able to induce climacteric ripening in ...201728493311
pectin from muskmelon (cucumis melo var. reticulatus) peels: extraction optimization and physicochemical properties.pectin derived from plant waste sources is currently focused as an economical and eco-friendly approach. optimization of pectin extraction from muskmelon peel by response surface methodology (rsm) was investigated in this study. box-behnken design (bbd) was used to identify the optimal level of the extraction variables such as time, ph and temperature. a second-order model equation for pectin extraction was obtained from multiple regression analysis of experimental data with the correlation coef ...201728452020
de novo comparative transcriptome analysis of genes involved in fruit morphology of pumpkin cultivars with extreme size difference and development of est-ssr markers.the genetic basis of fruit size and shape was investigated for the first time in cucurbita species and genetic loci associated with fruit morphology have been identified. although extensive genomic resources are available at present for tomato (solanum lycopersicum), cucumber (cucumis sativus), melon (cucumis melo) and watermelon (citrullus lanatus), genomic databases for cucurbita species are limited. recently, our group reported the generation of pumpkin (cucurbita pepo) transcriptome database ...201728435133
three phase partitioning, a scalable method for the purification and recovery of cucumisin, a milk-clotting enzyme, from the juice of cucumis melo var. reticulatus.cucumisin [ec 3.4.21.25] was first purified from cucumis melo var. reticulatus juice by three-phase partitioning (tpp). optimum purification parameters of the tpp system were determined as 60% ammonium sulfate saturation with 1.0:1.25 ratio of crude extract: t-butanol at ph and temperature of 8.0 and 20°c, respectively. cucumisin was purified with 4.61 purification fold and 156% activity recovery. the molecular weight of the recovered cucumisin was determined as 68.4kda and its isoelectric point ...201728428129
development of machine-vision system for gap inspection of muskmelon grafted seedlings.grafting robots have been developed in the world, but some auxiliary works such as gap-inspecting for grafted seedlings still need to be done by human. an machine-vision system of gap inspection for grafted muskmelon seedlings was developed in this study. the image acquiring system consists of a ccd camera, a lens and a front white lighting source. the image of inspected gap was processed and analyzed by software of halcon 12.0. the recognition algorithm for the system is based on principle of d ...201729267293
somatic embryogenesis of muskmelon (cucumis melo l.) and genetic stability assessment of regenerants using flow cytometry and issr markers.a new protocol for in vitro regeneration through direct somatic embryogenesis for two muskmelon cultivars (cucumis melo l., "mashhadi" and "eivanaki") is reported. somatic embryos were obtained culturing 4- and 8-day-old cotyledons, seeds, and hypocotyls on murashige and skoog medium supplemented with three different hormonal combinations never tested so far for melon (naphthoxyacetic acid (noa) + thidiazuron (tdz), noa + 6-banzylaminopurine (bap), and 2,4-dichlorophenoxyacetic acid (2,4-d) + n- ...201729248969
genome-wide identification and expression analysis of calcium‑dependent protein kinase and its related kinase gene families in melon (cucumis melo l.).the calcium-dependent protein kinase (cdpk) is a ser/thr protein kinase that plays vital roles in plant growth, development, and responses to multiple stresses. despite an important member of the stress responsive gene family, little is known about the evolutionary history and expression patterns of cdpk genes in melon. herein, a total of 18 cdpk genes and 7 cdpk-related protein kinases (crk) genes were identified in the melon genome via bioinformatic analysis, which were unevenly distributed ac ...201728437432
cloning and expression of class i chitinases in hami melon after penicillium infection.chitinases are important disease-related proteins that play critical roles in plant defense against disease. to investigate the function of chitinases in the resistance of hami melon to penicillium infection, the gene encoding chitinases, hmcht-2, was cloned and rt-pcr was used to measure expression levels of hmcht-2. when the hami melon was infected by penicillium sp after 0, 12, 36, 48, 60, and 72 h. the results showed that comparing to the control group, the time of expression levels reaching ...201728198499
impact of vat resistance in melon on viral epidemics and genetic structure of virus populations.cultivar choice is at the heart of cropping systems and resistant cultivars should be at the heart of disease management strategies whenever available. they are the easiest, most efficient and environmentally friendly way of combating viral diseases at the farm level. among the melon genetic resources, vat is a unique gene conferring resistance to both the melon aphid aphis gossypii and the viruses it carries. the 'virus side' of this pleiotropic phenotype is seldom regarded as an asset for viru ...201728587865
growth of salmonella spp. and escherichia coli o157:h7 on fresh-cut fruits stored at different temperatures.the objective of this work was to determine the growth potential of salmonella spp. and escherichia coli o157:h7 on fresh-cut honeydew melon, cantaloupe, watermelon, pitaya, mango, papaya, and pineapple stored at 5°c, 13°c, and 25°c. the results showed that both pathogens were able to grow on fresh-cut fruits except fresh-cut pineapple at 13°c and 25°c. salmonella spp. grew more rapidly on fresh-cut honeydew melon, cantaloupe, watermelon, and mango than did e. coli o157:h7 at 13°c. the growth of ...201728753059
pathological interrelations of soil-borne diseases in cucurbits caused by fusarium species and meloidogyne incognita.pathological interrelations of two soil-borne diseases in cucurbits (watermelon, oriental melon, shintosa and cucumber) caused by fusarium isolates (fi) and the root-knot nematode (rkn), meloidogyne incognita were characterized by the fusarium disease severity index (di), rkn gall index (gi) and eggmass index (ei) in inoculation tests using fi and rkn. virulence of fi as determined by di at 4 weeks after inoculation was mostly in the higher order of fusarium proliferatum f6, f5 and fusarium oxys ...201728811758
chemometric evaluation of the volatile profile of probiotic melon and probiotic cashew juice.the aim of this study was to evaluate the influence of the lactic acid fermentation on volatile compounds of melon and cashew apple juices. the effect of the fermentation processing on the volatile profile of probiotic juices was assessed by hs-spme/gc-ms coupled to chemometrics with 67.9% and 81.0% of the variance in the first principal component for melon and cashew juices, respectively. the lactobacillus casei fermentation imparted a reduction of ethyl butanoate, ethyl-2-methylbutirate, and e ...201728784506
potential reasons for prevalence of fusarium wilt in oriental melon in korea.this study aims to examine the potential reasons for the current prevalence of the fusarium wilt in the oriental melon. twenty-seven fusarium isolates obtained from oriental melon greenhouses in 2010-2011 were identified morphologically and by analysis of elongation factor-1 alpha gene (ef-1α) and internal transcribed spacer (its) rdna sequences as 6 fusarium species (8 isolates of f. oxysporum, 8 f. commune, 5 f. proliferatum, 3 f. equiseti, 2 f. delphinoides, and 1 f. andiyazi), which were cla ...201728592944
resistance to tomato leaf curl new delhi virus in melon is controlled by a major qtl located in chromosome 11.identification of three genomic regions and underlying candidate genes controlling the high level of resistance to tolcndv derived from a wild melon. snp markers appropriated for mas management of resistance. tomato leaf curl new delhi virus (tolcndv) is a bipartite begomovirus that severely affects melon crop (cucumis melo) in the main production areas of spain since 2012. in this work, we evaluated the degree of resistance of four accessions (two belonging to the subsp. agrestis var. momordica ...201728710536
genotyping-by-sequencing of a melon (cucumis melo l.) germplasm collection from a secondary center of diversity highlights patterns of genetic variation and genomic features of different gene pools.melon (cucumis melo l.) is one of the most important horticultural species, which includes several taxonomic groups. with the advent of next-generation sequencing, single nucleotide polymorphism (snp) markers are widely used in the study of genetic diversity and genomics.201728068911
new sources of resistance to leafminers (liriomyza sativae) in melon (cucumis melo l.) germplasm.leafminers (liriomyza sativae) are the main melon (cucumis melo l.) pests in northeast brazil, which is the main region for the production and export of the fruit in brazil. of the integrated management strategies available, genetic resistance is the best method of preventing damage by these insects. the aim of this study was to select sources of resistance to leafminers in melon germplasm. seven experiments were conducted in the laboratory, field, and greenhouse, with and without choice, using ...201728387878
non-invasive ethylene quantification in attached fruit headspace at 1 ppb by gas chromatography - mass spectrometry.ethylene is a gaseous plant hormone involved in defense, adaptations to environmental stress, and fruit ripening. its relevance to the latter makes its detection highly useful to physiologists interested in ripening onset. produced as a sharp peak during the respiratory burst, ethylene is biologically active at tens of nl·l(-1) . reliable quantification at such concentrations generally requires specialized instrumentation. here we present a rapid, high sensitivity method for detecting ethylene i ...201728370685
dissipation kinetics and pre-harvest residue limit of pyriofenone in oriental melon (cucumis melo var. makuwa) grown under regulated climatic conditions.a high-performance liquid chromatography-ultraviolet detection was used to estimate the disappearance rates as well as the pre-harvest residue limits of pyriofenone in oriental melon (cucumis melo var. makuwa) grown under greenhouse conditions in two different locations (a and b) in seongju, republic of korea. the identity of the compound in standard solution and representative field incurred samples was confirmed using liquid chromatography-tandem mass spectrometry. the method was validated in ...201728233340
itraq-based protein profiling and fruit quality changes at different development stages of oriental melon.oriental melon is one of the most popular crops for its nutritional and flavour quality. components that determine melon quality, such as sugar, colour, texture, flavour and aroma, among other factors, accumulate in different developmental stages. thus, correlating the proteomic profiles with the biochemical and physiological changes occurring in the oriental melon is very important for advancing our understanding of oriental melon quality in the ripening processes.201728129739
characterization, phenolic compounds and functional properties of cucumis melo l. peels.the aim of this work is to investigate the phytochemical composition and functional properties of the melon peels, considered as a by-product. melon peels (maazoun cultivar) are rich in nutritional ingredients such as carbohydrates (69.77%) and ash (3.67%). they contain significant amounts of total dietary fibers (41.69%) and antioxidants as polyphenols and flavonoids (332mg/100g extract and 95.46mg/100g extract, respectively). the identification and the quantification of the phenolic compounds ...201727979149
anti-angiogenic potential of trypsin inhibitor purified from cucumis melo seeds: homology modeling and molecular docking perspective.melons have a good source of protease inhibitors. its fruit and seeds have been used as a traditional medicine. however, its effects on angiogenesis and mechanism of its action remain elusive. herein trypsin inhibitor from aqueous extract of c. melo seeds (ticms) was purified. its effects on different steps of angiogenesis were evaluated. also, we examined its effects on migration and angiogenesis of endothelial cells. three dimensional model of ticms protein was accurately built in which ticms ...201727965128
mapping resistance to alternaria cucumerina in cucumis melo.infection with alternaria cucumerina causes alternaria leaf blight (alb), a disease characterized by lesion formation on leaves, leading to substantial yield and quality losses in cucumis melo (melon). although fungicides are effective against alb, reduction in the frequency of application would be economically and environmentally beneficial. resistant melon lines have been identified but the genetic basis of this resistance has not been determined. a saturated melon genetic map was constructed ...201727868479
genome-wide single nucleotide polymorphism discovery and the construction of a high-density genetic map for melon (cucumis melo l.) using genotyping-by-sequencing.although genotyping-by-sequencing (gbs) enables the efficient and low-cost generation of large numbers of markers, the utility of resultant genotypes are limited, because they are enormously error-prone and contain high proportions of missing data. in this study, we generated single nucleotide polymorphism (snp) markers for 109 recombinant inbred lines of melon (cucumis melo l.) using the gbs approach and ordered them according to their physical position on the draft double haploid line dhl92 ge ...201728220139
development of a novel, sensitive, selective, and fast methodology to determine malondialdehyde in leaves of melon plants by ultra-high-performance liquid chromatography-tandem mass spectrometry.early production of melon plant (cucumis melo) is carried out using tunnels structures, where extreme temperatures lead to high reactive oxygen species production and, hence, oxidative stress. malondialdehyde (mda) is a recognized biomarker of the advanced oxidative status in a biological system. thus a reliable, sensitive, simple, selective, and rapid separative strategy based on ultra-high-performance liquid chromatography coupled to positive electrospray-tandem mass spectrometry (uplc-(+)esi- ...201728203476
comparative study of proteasome inhibitory, synergistic antibacterial, synergistic anticandidal, and antioxidant activities of gold nanoparticles biosynthesized using fruit waste materials.the aim of this study was to compare the biological synthesis of gold nanoparticles (aunps) generated using the aqueous extracts of outer oriental melon peel (omp) and peach. the synthesized omp-aunps and peach extract (pe)-aunps were characterized by ultraviolet-visible spectroscopy, field emission scanning electron microscopy, energy dispersive x-ray analysis, x-ray powder diffraction, fourier transform infrared spectroscopy, and thermogravimetric analysis. the surface plasmon resonance spectr ...201727695326
structural basis of cucumisin protease activity regulation by its propeptide.cucumisin [ec 3.4.21.25], a subtilisin-like serine endopeptidase, was isolated from melon fruit, cucumis melo l. mature cucumisin (67 kda, 621 residues) is produced by removal of the propeptide (10 kda, 88 residues) from the cucumisin precursor by subsequence processing. it is reported that cucumisin is inhibited by its own propeptide. the crystal structure of mature cucumisin is reported to be composed of three domains: the subtilisin-like catalytic domain, the protease-associated domain and th ...201727616715
biologically green synthesized silver nanoparticles as a facile and rapid label-free colorimetric probe for determination of cu(2+) in water samples.a highly sensitive and cost-effective colorimetric sensing platform for the selective trace analysis of cu(2+) ions was developed based on the accelerated etching of riboflavin stabilized silver nanoparticles (r/agnps). the r/agnps were prepared from the cucumis melo juice by a green chemistry approach. the bio-synthesized agnps were studied by uv-vis spectroscopy and showed an intense absorption band at 404nm that were further confirmed by ftir and eds analysis. simultaneous presence of cu(2+) ...201727565767
effect of ultrasonic processing on the changes in activity, aggregation and the secondary and tertiary structure of polyphenol oxidase in oriental sweet melon (cucumis melo var. makuwa makino).polyphenol oxidase (ppo) mainly contributes to the browning reaction of fruits and vegetables and causes serious damage to the quality of sweet melon products. however, traditional methods to inactivate browning may induce more unexpected risks than ultrasonic processing. meanwhile, there are no reports on the effect of ultrasound on ppo directly purified from sweet melon.201727342634
cucurbitacin b exerts anti-cancer activities in human multiple myeloma cells in vitro and in vivo by modulating multiple cellular pathways.cucurbitacin b (cub), a triterpenoid compound isolated from the stems of cucumis melo, has long been used to treat hepatitis and hepatoma in china. although its remarkable anti-cancer activities have been reported, the mechanism by which it achieves this therapeutic activity remains unclear. this study was designed to investigate the molecular mechanisms by which cub inhibits cancer cell proliferation. our results indicate that cub is a novel inhibitor of aurora a in multiple myeloma (mm) cells, ...201727418139
neuroprotective effect of cucumis melo var. flexuosus leaf extract on the brains of rats with streptozotocin-induced diabetes.the central nervous system is one of the most vulnerable organs affected by the oxidative stress associated with diabetes mellitus. healthy food provides an important source for antioxidants. therefore, the protective effect of cucumis melo var. flexuosus (c. melo var. flexuosus) leaf extract on the brains of diabetic rats was investigated. adult male albino rats divided into 5 groups of 6 rats each were assigned into a normal control group and four diabetic groups. diabetes was induced in rats ...201727488111
genetic variation and population structure of cucumber green mottle mosaic virus.cucumber green mottle mosaic virus (cgmmv) is a single-stranded, positive sense rna virus infecting cucurbitaceous plants. in recent years, cgmmv has become an important pathogen of cucurbitaceous crops including watermelon, pumpkin, cucumber and bottle gourd in china, causing serious losses to their production. in this study, we surveyed cgmmv infection in various cucurbitaceous crops grown in zhejiang province and in several seed lots purchased from local stores with the dot enzyme-linked immu ...201728054163
transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants.fusarium oxysporum f. sp. melonis snyd. & hans race 1.2 (fom1.2) is the most virulent and yield-limiting pathogen of melon (cucumis melo l.) worldwide. current information suggest that the resistance to race 1.2 is controlled by multiple recessive genes and strongly affected by the environment. rna-seq analysis was used to identify candidate resistance genes and to dissect the early molecular processes deployed during melon-fom1.2 interaction in the resistant doubled haploid line nad and in the ...201728367157
agroindustrial composts to reduce the use of peat and fungicides in the cultivation of muskmelon seedlings.environmental concerns about peat extraction in wetland ecosystems have increased. therefore, there is an international effort to evaluate alternative organic substrates for the partial substitution of peat. the aim of this work was to use different composts (c1-c10) obtained from the fruit and vegetable processing industry (pepper, carrot, broccoli, orange, artichoke residues, sewage sludge (citric and pepper) and vineyard pruning wastes) to produce added-value composts as growing media with su ...201727197924
ultrastructure of compatible and incompatible interactions in phloem sieve elements during the stylet penetration by cotton aphids in melon.resistance of the melon line tgr-1551 to the aphid aphis gossypii is based on preventing aphids from ingesting phloem sap. in electrical penetration graphs (epgs), this resistance has been characterized with a. gossypii showing unusually long phloem salivation periods (waveform e1) mostly followed by pathway activities (waveform c) or if followed by phloem ingestion (waveform e2), ingestion was not sustained for more than 10 minutes. stylectomy with aphids on susceptible and resistant plants was ...201728213963
review of scientific evidence of medicinal convoy plants in traditional persian medicine.one concept used in traditional persian medicine (tpm) for multidrug therapy is that of the convoy drug (mobadregh). according to tpm texts, convoy drugs are substances (or drugs), which facilitate the access of drugs or foods to the whole body or to specific organs. this study reviewed some convoy drugs presented in tpm, their biological effects, and their probable interactions with main drugs, considering the increased absorption through inhibition of p-glycoprotein (p-gp) efflux function, bio ...201727041871
transcriptome analysis of the oriental melon (cucumis melo l. var. makuwa) during fruit development.the oriental melon (cucumis melo l. var. makuwa) is one of the most important cultivated cucurbits grown widely in korea, japan, and northern china. it is cultivated because its fruit has a sweet aromatic flavor and is rich in soluble sugars, organic acids, minerals, and vitamins. in order to elucidate the genetic and molecular basis of the developmental changes that determine size, color, and sugar contents of the fruit, we performed de novo transcriptome sequencing to analyze the genes express ...201728070461
distinct mechanisms of the orange protein in controlling carotenoid flux.β-carotene adds nutritious value and determines the color of many fruits, including melon (cucumis melo). in melon mesocarp, β-carotene accumulation is governed by the orange gene (cmor) golden single-nucleotide polymorphism (snp) through a yet to be discovered mechanism. in arabidopsis (arabidopsis thaliana), or increases carotenoid levels by posttranscriptionally regulating phytoene synthase (psy). here, we identified a cmor nonsense mutation (cmor-lowβ) that lowered fruit β-carotene levels wi ...201727837090
novel edible oil sources: microwave heating and chemical properties.the aim of this work was to investigate the effect of various microwave heating times (1, 3, 5, 10, and 15min) on the chemical properties of novel edible oil sources, including mashhadi melon (cucumis melo var. iranians cv. mashhadi), iranian watermelon (citrullus lanatus cv. fire fon), pumpkin (cucurbita pepo subsp. pepo var. styriaca), and yellow apple (malus domestica cv. golden delicious) seed oils. the evaluated parameters were peroxide value (pv), conjugated diene (cd) and triene (ct) valu ...201728290292
genome-wide identification and comparative expression analysis of lea genes in watermelon and melon genomes.late embryogenesis abundant (lea) proteins are large and diverse group of polypeptides which were first identified during seed dehydration and then in vegetative plant tissues during different stress responses. now, gene family members of lea proteins have been detected in various organisms. however, there is no report for this protein family in watermelon and melon until this study. a total of 73 lea genes from watermelon (cllea) and 61 lea genes from melon (cmlea) were identified in this compr ...201728250580
genome wide characterization of simple sequence repeats in watermelon genome and their application in comparative mapping and genetic diversity analysis.microsatellite markers are one of the most informative and versatile dna-based markers used in plant genetic research, but their development has traditionally been difficult and costly. the whole genome sequencing with next-generation sequencing (ngs) technologies provides large amounts of sequence data to develop numerous microsatellite markers at whole genome scale. ssr markers have great advantage in cross-species comparisons and allow investigation of karyotype and genome evolution through h ...201627495254
oviposition preferences of pickleworm (lepidoptera: crambidae) in relation to a potential push-pull cropping management approach.pickleworm, diaphania nitidalis cramer (lepidoptera: crambidae), is a major pest of cucurbits. the current management approach for this pest is weekly insecticide applications. a push-pull cropping approach may be an alternative management practice and could reduce reliance on pesticides. one potential push-pull scenario is the use of squash (cucurbita pepo l.) as a trap crop and watermelon (citrullus lanatus [thunb.] matsum. & nakai) as a deterrent intercrop to manage pickleworm on cantaloupe ( ...201627118111
genome-wide identification and analysis of the sgr gene family in cucumis melo l.chlorophyll (chl) is present in many plant organs, and its metabolism is strongly regulated throughout plant development. understanding the fate of chl in senescent leaves or during fruit ripening is a complex process. the stay-green (sgr) protein has been shown to affect chl degradation. in this study, we used the conserved sequences of stay-green domain protein (np_567673) in arabidopsis thaliana as a probe to search sgr family genes in the genome-wide melon protein database. four candidate sg ...201627813562
effects of 20 selected fruits on ethanol metabolism: potential health benefits and harmful impacts.the consumption of alcohol is often accompanied by other foods, such as fruits and vegetables. this study is aimed to investigate the effects of 20 selected fruits on ethanol metabolism to find out their potential health benefits and harmful impacts. the effects of the fruits on ethanol metabolism were characterized by the concentrations of ethanol and acetaldehyde in blood, as well as activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in liver of mice. furthermore, potential hea ...201627043608
sequence variation of bemisia tabaci chemosensory protein 2 in cryptic species b and q: new dna markers for whitefly recognition.bemisia tabaci gennadius biotypes b and q are two of the most important worldwide agricultural insect pests. genomic sequences of type-2 b. tabaci chemosensory protein (btabcsp2) were cloned and sequenced in b and q biotypes, revealing key biotype-specific variations in the intron sequence. a q260 sequence was found specifically in q-btabcsp2 and cucumis melo ln692399, suggesting ancestral horizontal transfer of gene between the insect and the plant through bacteria. a cleaved amplified polymorp ...201626481237
identification of aphis gossypii glover (hemiptera: aphididae) biotypes from different host plants in north china.the cotton-melon aphid, aphis gossypii glover (hemiptera: aphididae), is a polyphagous species with a worldwide distribution and a variety of biotypes. north china is a traditional agricultural area with abundant winter and summer hosts of a. gossypii. while the life cycles of a. gossypii on different plants have been well studied, those of the biotypes of north china are still unclear.201626735973
mirna-mediated auxin signalling repression during vat-mediated aphid resistance in cucumis melo.resistance to aphis gossypii in melon is attributed to the presence of the single dominant r gene virus aphid transmission (vat), which is biologically expressed as antibiosis, antixenosis and tolerance. however, the mechanism of resistance is poorly understood at the molecular level. aphid-induced transcriptional changes, including differentially expressed mirna profiles that correspond to resistance interaction have been reported in melon. the potential regulatory roles of mirnas in vat-mediat ...201626437210
transmission of squash vein yellowing virus to and from cucurbit weeds and effects on sweetpotato whitefly (hemiptera: aleyrodidae) behavior.since 2003, growers of florida watermelon [citrullus lanatus (thunb.) matsum. and nakai] have periodically suffered large losses from a disease caused by squash vein yellowing virus (sqvyv), which is transmitted by the whitefly middle east-asia minor 1 (meam1), formerly bemisia tabaci (gennadius) biotype b. common cucurbit weeds like balsam apple (momordica charantia l.) and smellmelon [cucumis melo var. dudaim (l.) naud.] are natural hosts of sqvyv, and creeping cucumber (melothria pendula l.) ...201627400705
rapid ester biosynthesis screening reveals a high activity alcohol-o-acyltransferase (aatase) from tomato fruit.ethyl and acetate esters are naturally produced in various yeasts, plants, and bacteria. the biosynthetic pathways that produce these esters share a common reaction step, the condensation of acetyl/acyl-coa with an alcohol by alcohol-o-acetyl/acyltransferase (aatase). recent metabolic engineering efforts exploit aatase activity to produce fatty acid ethyl esters as potential diesel fuel replacements as well as short- and medium-chain volatile esters as fragrance and flavor compounds. these effor ...201626814045
differential colonization dynamics of cucurbit hosts by erwinia tracheiphila.bacterial wilt is one of the most destructive diseases of cucurbits in the midwestern and northeastern united states. although the disease has been studied since 1900, host colonization dynamics remain unclear. cucumis- and cucurbita-derived strains exhibit host preference for the cucurbit genus from which they were isolated. we constructed a bioluminescent strain of erwinia tracheiphila (tedcu10-bl#9) and colonization of different cucurbit hosts was monitored. at the second-true-leaf stage, cuc ...201626926487
survival and growth of listeria monocytogenes on whole cantaloupes is dependent on site of contamination and storage temperature.whole cantaloupes (cucumis melo l.), marketed as 'rocky ford', were implicated in a large multi-state outbreak of listeriosis in the united states in 2011; however, survival and growth of listeria monocytogenes on whole cantaloupes remains relatively unexplored. the research presented here evaluated three different storage temperatures, two sites of contamination of cantaloupes, and two cantaloupe varieties to determine their effect on the survival of l. monocytogenes. 'athena' and 'rocky ford' ...201627376678
microbial indicator profiling of fresh produce and environmental samples from farms and packing facilities in northern mexico.to compare microbiological indicator and pathogen contamination among different types of fresh produce and environmental samples along the production chain, 636 samples of produce (rinsates from cantaloupe melons, jalapeño peppers, and tomatoes) and environmental samples (rinsates from hands of workers, soil, and water) were collected at four successive steps in the production process (from the field before harvest through the packing facility) on 11 farms in northern mexico during 2011 and 2012 ...201627357040
partial biological and molecular characterization of a cucumber mosaic virus isolate naturally infecting cucumis melo in iran.melon seedlings showing systemic chlorotic spots and mosaic symptoms were collected in central part of iran, and a virus was isolated from diseased plants by mechanical inoculation. the virus systemically infected the most inoculated test plants by inducing mosaic symptoms, while, in the members of fabaceae family and chenopodium quinoa induced local lesions. agar gel diffusion test using a polyclonal antiserum against a squash cucumber mosaic virus (cmv) isolate showed the presence of cmv in th ...201627366772
transcriptomic profiling of melon necrotic spot virus-infected melon plants revealed virus strain and plant cultivar-specific alterations.viruses are among the most destructive and difficult to control plant pathogens. melon (cucumis melo l.) has become the model species for the agriculturally important cucurbitaceae family. approaches that take advantage of recently developed genomic tools in melon have been extremely useful for understanding viral pathogenesis and can contribute to the identification of target genes for breeding new resistant cultivars. in this work, we have used a recently described melon microarray for transcr ...201627267368
heterologous expression and biochemical characterization of two lipoxygenases in oriental melon, cucumis melo var. makuwa makino.lipoxygenases (loxs) are a class of non-heme iron-containing dioxygenases that catalyse oxidation of polyunsaturated fatty acids to produce hydroperoxidation that are in turn converted to oxylipins. although multiple isoforms of loxs have been detected in several plants, loxs in oriental melon have not attracted much attention. two full-length lox cdna clones, cmlox10 and cmlox13 which have been isolated from oriental melon (cucumis melo var. makuwa makino) cultivar "yumeiren", encode 902 and 90 ...201627101009
assessment of the microbiological safety of precut fruit from retail and catering premises in the united kingdom.fresh fruit has been associated with a number of foodborne outbreaks in recent years. in particular, a large outbreak of listeriosis in the united states in 2011 was associated with consumption of cantaloupe melon, and an outbreak of salmonella newport in the united kingdom and europe (also in 2011) was linked to watermelon consumption. a study of precut fruit products from catering and retail premises in the united kingdom was, therefore, carried out to assess their microbiological safety. betw ...201627052864
vat, an amazing gene conferring resistance to aphids and viruses they carry: from molecular structure to field effects.we review half a century of research on cucumis melo resistance to aphis gossypii from molecular to field levels. the vat gene is unique in conferring resistance to both a. gossypii and the viruses it transmits. this double phenotype is aphid clone-dependent and has been observed in 25 melon accessions, mostly from asia. it is controlled by a cluster of genes including cc-nlr, which has been characterized in detail. copy-number polymorphisms (for the whole gene and for a domain that stands out i ...201627725823
multicolor fluorescence imaging as a candidate for disease detection in plant phenotyping.the negative impact of conventional farming on environment and human health make improvements on farming management mandatory. imaging techniques are implemented in remote sensing for monitoring crop fields and plant phenotyping programs. the increasingly large size and complexity of the data obtained by these techniques, makes the implementation of powerful mathematical tools necessary in order to identify informative parameters and to apply them in precision agriculture. multicolor fluorescenc ...201627994607
gibberellins producing bacillus methylotrophicus ke2 supports plant growth and enhances nutritional metabolites and food values of lettuce.the nutritional quality of green leafy vegetables can be enhanced by application of plant beneficial micro-organisms. the present study was aimed to increase the food values of lettuce leaves by bacterial treatment. we isolated bacterial strain ke2 from kimchi food and identified as bacillus methylotrophicus by phylogenetic analysis. the beneficial effect of b. methylotrophicus ke2 on plants was confirmed by increasing the percentage of seed germination of lactuca sativa l., cucumis melo l., gly ...201627721133
arsenic uptake by muskmelon (cucumis melo) plants from contaminated water.arsenic is a carcinogenic element that occurs naturally in the environment. high levels of arsenic are found in water in some parts of the world, including texas. the aims of this study were to determine the distribution of arsenic in muskmelon (cucumis melo) plants accumulated from arsenic spiked water and to observe effects on plant biomass. plants were grown and irrigated using water spiked with variable concentrations of arsenic. inductively coupled plasma mass spectrometry was used to quant ...201627460822
the relationship between cmadhs and the diversity of volatile organic compounds of three aroma types of melon (cucumis melo).alcohol dehydrogenase (adh) plays an important role in aroma volatile compounds synthesis of plants. in this paper, we tried to explore the relationship between cmadhs and the volatile organic compounds (vocs) in oriental melon. three different aroma types of melon were used as materials. the principle component analysis of three types of melon fruit was conducted. we also measured the cmadhs expression level and enzymatic activities of adh and alcohol acyl-transferase (aat) on different stages ...201627445845
first record of drosophila buzzatii (patterson & wheeler) (diptera: drosophilidae) emerging from a non-cactus host.drosophila buzzatii (patterson & wheeler), a typical cactophilic species of the repleta group, is registered for the first time emerging from melon (cucumis melo) in western argentina. the analysis of inversion polymorphism and genetic diversity of mitochondrial cytochrome oxidase subunit i gene (mtcoi) provided additional evidence that corroborated the presence of a high proportion of d. buzzatii among the flies emerged from melon. this finding set the scenario for a broader range of possible h ...201626960546
microbiological stability and quality of pulsed light treated cantaloupe (cucumis melo l. reticulatus cv. glamour) based on cut type and light fluence.the aim of this study was to investigate the effect of cut type and pulsed light (pl) fluence on microbiological stability and quality of fresh-cut cantaloupes. fresh-cut cantaloupes with various cut types (cuboid, triangular prism and sphere) were treated with pl technology at 6 j/cm(2). samples were exposed to pl treatment at fluences of 2.7, 7.8, 11.7 and 15.6 j/cm(2) followed by storage at 4 ± 1 °c for 28 days. microbiological quality, headspace composition, firmness, colour, ph, titratable ...201627413207
spray dried melon seed milk powder: physical, rheological and sensory properties.melon seed milk (msm) powder was produced by aiming to get alternative vegetable milk from crushed kırkağaç (cucumis melo subsp. melo cv. kırkağaç) and çeşme (c. melo subsp. melo cv. çeşme) type melon seeds. msm was converted to powder form via spray dryer at inlet air temperature of 150 °c, air flow rate of 473 l · h(-1), aspiration ratio of 24 m(3) · h(-1)and feed flow rate of 8 ml · min(-1) in order to extend the shelf life and usage area. the moisture content and water activity of samples ch ...201627407206
a comparative study on the effectiveness of ozonated water and peracetic acid in the storability of packaged fresh-cut melon.ozonated water and peracetic acid were tested as sanitizers to enhance the storability of fresh-cut melon cubes. sanitizers were also combined with suitable packaging materials (polypropylene and polylactic acid based plastic films). fresh-cut melon cubes were stored at 4 °c for up to 7 days. ozonated water and peracetic acid treatments were given by dipping cubes into 0.8 ppm o3 and 100 ppm tsunami 100™ solutions, respectively, for 3 min. both sanitizers exhibited efficiency in reducing the tot ...201627407201
mapping the flavor contributing traits on "fengwei melon" (cucumis melo l.) chromosomes using parent resequencing and super bulked-segregant analysis.we used a next-generation high-throughput sequencing platform to resequence the xinguowei and shouxing melon cultivars, the parents of fengwei melon. we found 84% of the reads (under a coverage rate of "13×") placed on the reference genome dhl92. there were 2,550,000 single-nucleotide polymorphisms and 140,000 structural variations in the two genomes. we also identified 1,290 polymorphic genes between xinguowei and shouxing. we combined specific length amplified fragment sequencing (slaf-seq) an ...201626840947
cucumis melo endornavirus: genome organization, host range and co-divergence with the host.a high molecular weight dsrna was isolated from a cucumis melo l. plant (referred to as 'cl01') of an unknown cultivar and completely sequenced. sequence analyses showed that dsrna is associated with an endornavirus for which a name cucumis melo endornavirus (cmev) is proposed. the genome of cmev-cl01 consists of 15,078 nt, contains a single, 4939 codons-long orf and terminates with a stretch of 10 cytosine residues. comparisons of the putative cmev-encoded polyprotein with available references ...201626805037
de novo transcriptome analysis of cucumis melo l. var. makuwa.oriental melon (cucumis melo l. var. makuwa) is one of six subspecies of melon and is cultivated widely in east asia, including china, japan, and korea. although oriental melon is economically valuable in asia and is genetically distinct from other subspecies, few reports of genome-scale research on oriental melon have been published. we generated 30.5 and 36.8 gb of raw rna sequence data from the female and male flowers, leaves, roots, and fruit of two oriental melon varieties, korean landrace ...201626743902
whole genome re-sequencing and characterization of powdery mildew disease-associated allelic variation in melon.powdery mildew is one of the most common fungal diseases in the world. this disease frequently affects melon (cucumis melo l.) and other cucurbitaceous family crops in both open field and greenhouse cultivation. one of the goals of genomics is to identify the polymorphic loci responsible for variation in phenotypic traits. in this study, powdery mildew disease assessment scores were calculated for four melon accessions, 'scnu1154', 'edisto47', 'mr-1', and 'pmr5'. to investigate the genetic varia ...201627311063
the alcohol dehydrogenase gene family in melon (cucumis melo l.): bioinformatic analysis and expression patterns.alcohol dehydrogenases (adh), encoded by multigene family in plants, play a critical role in plant growth, development, adaptation, fruit ripening and aroma production. thirteen adh genes were identified in melon genome, including 12 adhs and one formaldehyde dehydrogenease (fdh), designated cmadh1-12 and cmfdh1, in which cmadh1 and cmadh2 have been isolated in cantaloupe. adh genes shared a lower identity with each other at the protein level and had different intron-exon structure at nucleotide ...201627242871
genome-wide investigation of the nac transcription factor family in melon (cucumis melo l.) and their expression analysis under salt stress.82 melon nac (cmnac) genes were identified in melon. we putatively identified the function of cmnac gene in melon under salt stress. nac transcription factor proteins play important roles in many biological processes, including plant development and stress responses. to date, few full-length melon nac proteins have been identified. in this study, 82 melon nac (cmnac) genes were identified in the cucumis melo l. genome. by interrogating our cdna libraries and transcriptome data from melon under s ...201627229006
genetic characterization of turkish snake melon (cucumis melo l. subsp. melo flexuosus group) accessions revealed by ssr markers.snake melon is an important cucurbit crop especially in the southeastern and the mediterranean region of turkey. it is consumed as fresh or pickled. the production is mainly done with the local landraces in the country. turkey is one of the secondary diversification centers of melon and possesses valuable genetic resources which have different morphological characteristics in case of snake melon. genetic diversity of snake melon genotypes collected from different regions of turkey and reference ...201627193591
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