Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| production and partial characterization of high molecular weight extracellular alpha-amylase from thermoactinomyces vulgaris isolated from egyptian soil. | optimizing production of alpha-amylase production by thermoactinomyces vulgaris isolated from egyptian soil was studied. the optimum incubation period, temperature and initial ph of medium for organism growth and enzyme yield were around 24 h, 55 degrees c and 7.0, respectively. maximum alpha-amylase activity was observed in a medium containing starch as carbon source. the other tested carbohydrates (cellulose, glucose, galactose, xylose, arabinose, lactose and maltose) inhibited the enzyme prod ... | 2011 | 21630576 |
| enzymatic synthesis of novel branched sugar alcohols mediated by the transglycosylation reaction of pullulan-hydrolyzing amylase ii (tva ii) cloned from thermoactinomyces vulgaris r-47. | transglycosylation reactions are useful for preserving a specific sugar structure during the synthesis of branched oligosaccharides. we have previously reported a panosyl unit transglycosylation reaction by pullulan-hydrolyzing amylase ii (tva ii) cloned from thermoactinomyces vulgaris r-47 (tonozuka et al., carbohydr. res., 1994, 261, 157-162). the acceptor specificity of the tva ii transglycosylation reaction was investigated using pullulan as the donor and sugar alcohols as the acceptor. tva ... | 2011 | 21722879 |
| mouse model of hypersensitivity pneumonitis after inhalation exposure to different microbial antigens associated with organic dusts. | the aim of this study was to reproduce in laboratory conditions hypersensitivity pneumonitis (hp) pathogenesis in a new animal model predictive of the human response, and to select the microbial antigen associated with organic dust that exerts the strongest pathogenic effect on the respiratory organ. to achieve this goal, mice of the strain c57bl/6j prone to fibrosis were exposed for 1 hour daily up to 28 days to the inhalation of aerosols of each of the 5 microbial components of organic dusts w ... | 2011 | 21736281 |
| involvement of propeptides in formation of catalytically active metalloproteinase from thermoactinomyces sp. | the metalloproteinase from thermoactinomyces sp. 27a (mpr) represents secretory thermolysin-like metalloproteinases of the m4 family. the thermoactinomyces enzyme is synthesized as a precursor consisting of a signal peptide, n-terminal propeptide, mature region, and c-terminal propeptide. the functional molecule lacks the signal peptide, n-terminal propeptide, and c-terminal propeptide, which indicates the processing of these regions. until now, it remained unclear if the n-terminal propeptide i ... | 2011 | 21675948 |
| inoculation of food waste with the thermo-tolerant lipolytic actinomycete thermoactinomyces vulgaris a31 and maturity evaluation of the compost. | the ability of the thermo-tolerant lipolytic actinomycete, thermoactinomyces vulgaris a31, to efficiently decompose food waste into mature compost was studied. using a range of chemical parameters (ph, total organic carbon content (toc), total nitrogen content, c/n ratio), co(2) evolution, enzymatic activities (dehydrogenase, polyphenol oxidase, urease) and germination assays, the composition, stability and maturity of the compost produced were assessed. inoculation reduced crude fat and decreas ... | 2010 | 20462753 |
| [case of bird-related hypersensitivity pneumonitis in a farmer]. | a 48-year-old female farmer presented to our hospital with shortness of breath and cough. her partial pressure of arterial oxygen (pao2) level on room air was 58.4torr, and chest computed tomography (ct) showed centrilobular ground-glass micronodules. she was admitted for further evaluation. after admission, symptoms, pao2, and radiological findings improved without any treatment; however, her symptoms recurred when she returned to work. exposure to her house did not elicit any symptomatic respo ... | 2010 | 21226310 |
| the microbial signature of aerosols produced during the thermophilic phase of composting. | the microbial diversity of bioaerosols released during operational activities at composting plants is poorly understood. identification of bacteria and fungi present in such aerosols is the prerequisite for the definition of microbial indicators that could be used in dispersal and exposure studies. | 2010 | 19602015 |
| alkalithermophilic actinomycetes in a subtropical area of jujuy, argentina. | the objective of this study was to examine the alkalithermophilic actinomycete communities in the subtropical environment of jujuy, argentina, characterized by sugarcane crops. laceyella putida, laceyella sacchari, thermoactinomyces intermedius, thermoactinomyces vulgaris and thermoflavimicrobium dichotomicum were isolated on the media with novobiocin, from sugar cane plants and renewal rhizospheres, and grass and wood soils. soil ph was almost neutral or lightly alkaline, except for grass soil ... | 2009 | 19623902 |
| respiratory health effects and immunological response to thermoactinomyces among sugar cane workers in nicaragua. | specific sensitization and respiratory effects associated with the inhalation of sugar cane dust were evaluated in a group of 51 nicaraguan workers exposed to bagasse. a questionnaire interview, lung function test, serum precipitin tests for thermoactinomyces sacchari and t. vulgaris, and immunoglobulin e tests for specific environmental allergens were performed for each worker. twenty-one workers reported at least one respiratory symptom and 16 reported possible symptoms of bagassosis. six work ... | 2009 | 19650579 |
| identification and characterization of a novel spore-associated subtilase from thermoactinomyces sp. cdf. | a gene encoding a spore-associated subtilase, designated protease cdf, was cloned from thermoactinomyces sp. cdf and expressed in escherichia coli. the enzyme gene is translated as a proform consisting of a 94 aa propeptide and a 283 aa mature protease domain. phylogenetic analysis revealed that this enzyme belonged to the subtilisin family, but could not be grouped into any of its six known subfamilies. the mature protease cdf has an unusually high content of charged residues, which are mainly ... | 2009 | 19696109 |
| desmospora activa gen. nov., sp. nov., a thermoactinomycete isolated from sputum of a patient with suspected pulmonary tuberculosis, and emended description of the family thermoactinomycetaceae matsuo et al. 2006. | a novel gram-positive, aerobic, catalase-positive, filamentous micro-organism, designated strain immib l-1269(t), originating from sputum was characterized using phenotypic and molecular taxonomic methods. it showed cell-wall chemotype iii, phospholipid type pii (with phosphatidylethanolamine as the diagnostic phospholipid) and contained an unsaturated menaquinone with seven isoprene units (mk-7) as the predominant isoprenoid quinone. it synthesized long-chain cellular fatty acids of the straigh ... | 2009 | 19244421 |
| detection and molecular characterization of filamentous actinobacteria and thermoactinomycetes present in water-damaged building materials. | in this study the dominant filamentous actinobacteria occurring in water-damaged building materials were detected by culture and characterized by automated ribotyping and 16s rrna gene sequencing. fifty-two samples were taken from 20 water-damaged houses in four different countries. a total of 122 bacterial isolates were analyzed. actinobacteria or thermoactinomycetes were present in 48% of the samples. the dominant genus was streptomyces (58% of isolates), followed by thermoactinomyces (23%), l ... | 2009 | 19302504 |
| degradation of rutin by thermoactinomyces vulgaris and other thermophilic compost isolates. | the mutagenic effects of rutin and quercetin have aroused the interest of many investigators. to develop the microbial degradation of rutin, a thermophilic actinomycete, which could hydrolyze rutin, was isolated from compost soil. the taxonomical characteristics of this thermophilic actinomycete were examined and identified as thermoactinomyces vulgaris pu18-2. after cultivation of t. vulgaris pu18-2 in the rutin-cyc medium for 60 h, the culture filtrate had a rutin-degrading ability, but the ce ... | 2009 | 19489631 |
| crystal structures of open and closed forms of cyclo/maltodextrin-binding protein. | the crystal structures of thermoactinomyces vulgaris cyclo/maltodextrin-binding protein (tvucmbp) complexed with alpha-cyclodextrin (alpha-cd), beta-cyclodextrin (beta-cd) and maltotetraose (g4) have been determined. a common functional conformational change among all solute-binding proteins involves switching from an open form to a closed form, which facilitates transporter binding. escherichia coli maltodextrin-binding protein (ecombp), which is structurally homologous to tvucmbp, has been det ... | 2009 | 19490104 |
| diversity of bacteria and fungi in aerosols during screening in a green waste composting plant. | this article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. the structure of the microbial community was examined using a fingerprint technique and dna libraries. the results show: (i) the very high diversity of bacteria and fungi in aerosols produced during the composting screening stage ... | 2009 | 19533230 |
| tryptamine derived amides with thiazole ring system from thermoactinomyces strain ta66-2. | a moderately thermophilic actinomycete strain, which was identified as thermoactinomyces strain ta66-2, was isolated from hot-spring water. fermentation, followed by solvent partition and chromatographic separations, resulted in the isolation of two new and two known molecules. the structures of the new compounds were elucidated as 2-(1-propionylaminoethyl)thiazole-4-carboxylic acid [2-(1h-indol-3-yl)ethyl]amide and 2-(1-acetylaminoethyl)thiazole-4-carboxylic acid [2-(1h-indol-3-yl)-ethyl]amide ... | 2008 | 18041008 |
| [a case of hypersensitity pneumonitis due to the contamination of a dehumidifier by thermoactinomyces vulgaris]. | a 22-year-old man was admitted to our hospital because of high fever and shortness of breath. a chest ct showed centrilobular nodules and ground-glass attenuation in both lungs. transbronchial lung biopsy specimens showed alveolitis including the infiltration of mononuclear cells and granulomas. he responded to treatment, but upon returning home the radiological findings and clinical symptoms reappeared. we focused on a dehumidifier because it had been used continuously in his room. thermoactino ... | 2008 | 18260309 |
| structural principles of the broad substrate specificity of thermoactinomyces vulgaris carboxypeptidase t--role of amino acid residues at positions 260 and 262. | an influence of residues at positions 260 and 262 on a broad substrate specificity of thermoactinomyces vulgaris carboxypeptidase t (cpt) has been studied by means of site-directed mutagenesis. the structure of the s1'-site of cpt is similar to those of pancreatic carboxypeptidases a (cpa) and b (cpb); however, the enzyme is capable of cleaving off c-terminal hydrophobic (like cpa), c-terminal positively charged (like cpb), and negatively charged residues. the spatial alteration of the s1' site ... | 2008 | 18515300 |
| leu254 residue and calcium ions as new structural determinants of carboxypeptidase t substrate specificity. | new determinants of thermoactinomyces vulgaris carboxypeptidase t (cpt) substrate specificity--structural calcium ions and leu254 residue--were found by means of steady-state kinetics and site-directed mutagenesis. the removal of calcium ions shifted the selectivity profile of hydrolysis of tripeptide substrates with c-terminal leu, glu, and arg from 64/1.7/1 to 162/1.3/1. substitution of the hydrophobic leu254 in cpt for polar asn did not change hydrolysis efficiency of substrates with c-termin ... | 2008 | 18991561 |
| cellular basis of chronic obstructive pulmonary disease in horses. | chronic obstructive pulmonary disease (copd) is an inflammatory obstructive disease of the airways characterized with hypersensitivity of the airway tissues to various allergens, most commonly the fungi contained in the poor-quality hay and straw bedding-saccharopolyspora rectivirgula, aspergillus fumigatus, and thermoactinomyces vulgaris. it is manifested clinically in middle-aged horses with recurrent episodes of dyspnea, chronic cough, and their reduced athletic and working capacity. pulmonar ... | 2007 | 17280899 |
| temperature impacts the multiple attack action of amylases. | the action pattern of several amylases was studied at 35, 50, and 70 degrees c using potato amylose, a soluble (red starch) and insoluble (cross-linked amylose) chromophoric substrate. with potato amylose as substrate, bacillus stearothermophilus alpha-amylase (bsta) and porcine pancreatic alpha-amylase displayed a high degree of multiple attack (dma, i.e., the number of bonds broken during the lifetime of an enzyme-substrate complex minus one), the fungal alpha-amylase from aspergillus oryzae a ... | 2007 | 17309295 |
| structural basis for cyclodextrin recognition by thermoactinomyces vulgaris cyclo/maltodextrin-binding protein. | the crystal structure of a thermoactinomyces vulgaris cyclo/maltodextrin-binding protein (tvucmbp) complexed with gamma-cyclodextrin has been determined. like escherichia coli maltodextrin-binding protein (ecombp) and other bacterial sugar-binding proteins, tvucmbp consists of two domains, an n- and a c-domain, both of which are composed of a central beta-sheet surrounded by alpha-helices; the domains are joined by a hinge region containing three segments. gamma-cyclodextrin is located at a clef ... | 2007 | 17371546 |
| val326 of thermoactinomyces vulgaris r-47 amylase ii modulates the preference for alpha-(1,4)- and alpha-(1,6)-glycosidic linkages. | thermoactinomyces vulgaris r-47 alpha-amylase ii (tva ii) catalyzes not only the hydrolysis of alpha-(1,4)- and alpha-(1,6)-glycosidic linkages but also transglycosylation. the subsite +1 structure of alpha-amylase family enzymes plays important roles in substrate specificity and transglycosylation activity. we focused on the amino acid residue at the 326th position based on information on the primary structure and crystal structure, and replaced val with ala, ile, or thr. the v326a mutant favor ... | 2007 | 17400040 |
| structural principles of the wide substrate specificity of thermoactinomyces vulgaris carboxypeptidase t. reconstruction of the carboxypeptidase b primary specificity pocket. | site-directed mutagenesis in the active site of thermoactinomyces vulgaris carboxypeptidase t (cpt), which is capable of hydrolyzing both hydrophobic and positively charged substrates, resulted in five mutants: cpt1 (a243g), cpt2 (d253g/t255d), cpt3 (a243g/d253g/t255d), cpt4 (g207s/a243g/d253g/t255d), and cpt5 (g207s/a243g/t250a/d253g/t255d). these mutants step-by-step reconstruct the primary specificity pocket of carboxypeptidase b (cpb), which is capable of cleaving only positively charged c-t ... | 2007 | 17511606 |
| polyester-degrading thermophilic actinomycetes isolated from different environment in taiwan. | thermophilic actinomycetes strains were isolated from various environment in taiwan and screened for degradation of poly(ethylene succinate) (pes), poly(epsilon-caprolactone) (pcl) and/or poly(beta-hydroxybutyrate) (phb) by the clear-zone method. out of 341 strains of thermophilic actinomycetes, 105 isolates were phb-degraders (30.8%), 198 isolates were pcl-decomposers (58.1%), and 99 isolates could degrade pes (29.0%). furthermore, 77 isolates could degrade both phb and pcl (22.6%), 35 isolates ... | 2007 | 17653512 |
| mg2+ decreases arrhenius energies of activation for high temperature catalysis of phosphatases in thermoactinomyces vulgaris. | the nonspecific acid and alkaline phosphatases of thermoactinomyces vulgaris were found to be optimally active at 65 degrees c and 70 degrees c, respectively, indicating the thermophilic nature of these enzymes in this obligate thermophile. mg(2+), when added in the assay mixture (in the form of mgcl(2)), increased the specific activities of these enzymes without affecting their respective temperature optima. this divalent cation decreased the arrhenius energies of activation (e ( a )) of both a ... | 2007 | 17657541 |
| structure of a complex of thermoactinomyces vulgaris r-47 alpha-amylase 2 with maltohexaose demonstrates the important role of aromatic residues at the reducing end of the substrate binding cleft. | thermoactinomyces vulgaris r-47 alpha-amylase 2 (tvaii) can efficiently hydrolyze both starch and cyclomaltooligosaccharides (cyclodextrins). the crystal structure of an inactive mutant tvaii in a complex with maltohexaose was determined at a resolution of 2.1a. tvaii adopts a dimeric structure to form two catalytic sites, where substrates are found to bind. at the catalytic site, there are many hydrogen bonds between the enzyme and substrate at the non-reducing end from the hydrolyzing site, bu ... | 2006 | 16564038 |
| novel thermostable serine collagenase from thermoactinomyces sp. 21e: purification and some properties. | a thermophilic actinomycete strain thermoactinomyces sp. 21e producing a highly thermostable serine collagenase was isolated from bulgarian soil. the collagenase, produced extracellular by thermoactinomyces sp. 21e, was purified to homogeneity by heat treatment, ultrafiltration, saturation with ammonium sulfate and gel filtration chromatography with a 101-fold increase in specific activity and 58% recovery. the collagenase has a relative molecular mass of 50000 by sds-page. the optimum temperatu ... | 2006 | 16847831 |
| occurrence and characterization of actinobacteria and thermoactinomycetes isolated from pulp and board samples containing recycled fibres. | the aim of this study was to characterize the actinobacterial population present in pulps and boards containing recycled fibres. a total of 107 isolates was identified on the basis of their pigmentation, morphological properties, fatty acid profiles and growth temperature. of the wet pulp and water sample isolates (n=87), 74.7% belonged to the genus streptomyces, 17.2% to nocardiopsis and 8.0% to thermoactinomycetes, whereas all the board sample isolates (n=20) were thermoactinomycetes. the iden ... | 2006 | 16292555 |
| complexes of thermoactinomyces vulgaris r-47 alpha-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with alpha-(1,6) glycosidic linkages. | thermoactinomyces vulgaris r-47 alpha-amylase 1 (tvai) has unique hydrolyzing activities for pullulan with sequence repeats of alpha-(1,4), alpha-(1,4), and alpha-(1,6) glycosidic linkages, as well as for starch. tvai mainly hydrolyzes alpha-(1,4) glycosidic linkages to produce a panose, but it also hydrolyzes alpha-(1,6) glycosidic linkages with a lesser efficiency. x-ray structures of three complexes comprising an inactive mutant tvai (d356n or d356n/e396q) and a pullulan model oligosaccharide ... | 2005 | 16302977 |
| proposal of the genus thermoactinomyces sensu stricto and three new genera, laceyella, thermoflavimicrobium and seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. | phylogenetic analysis based on 16s rrna gene sequences revealed that thermoactinomyces species with validly published names can be assigned to four clusters or lineages. the type strains of thermoactinomyces sacchari and thermoactinomyces putidus were differentiated from the type strains of thermoactinomyces vulgaris and thermoactinomyces intermedius by the predominant menaquinone and fatty acid profiles. the type strains of thermoactinomyces dichotomicus and thermoactinomyces peptonophilus form ... | 2005 | 15653907 |
| in vitro refolding of carboxypeptidase t precursor from thermoactinomyces vulgaris obtained in escherichia coli as cytoplasmic inclusion bodies. | carboxypeptidase t precursor from thermoactinomyces vulgaris, which fails to contain its own leader peptide, has been expressed in escherichia coli as insoluble cytoplasmic inclusion bodies. the yield of a washed recombinant protein from 1 l of culture liquid was about 60 mg. the obtained inclusion bodies were denatured in 6 m guanidine-hcl and then renatured by a rapid dilution. the important role of calcium for the complete stabilization of the refolded carboxypeptidase t precursor was establi ... | 2005 | 15721771 |
| degradation of keratin and collagen containing wastes by newly isolated thermoactinomycetes or by alkaline hydrolysis. | the aim of this study was to develop a method for microbial degradation of indigenous keratin wastes and to compare it with a method of alkaline hydrolysis. | 2005 | 15836735 |
| mechercharmycins a and b, cytotoxic substances from marine-derived thermoactinomyces sp. ym3-251. | a new cytotoxic substance named mechercharmycin a was isolated from marine-derived thermoactinomyces sp. ym3-251. the structure of mechercharmycin a was determined by an x-ray crystallographic analysis to be cyclic peptide-like and bearing four oxazoles and a thiazole. mechercharmycin b, a linear congener of mechercharmycin a, was also isolated from the same bacterium. mechercharmycin a exhibited relatively strong antitumor activity, whereas mechercharmycin b exhibited almost no such activity. | 2005 | 15981418 |
| [the phylogenetic diversity of aerobic organotrophic bacteria from the dagan high-temperature oil field]. | the distribution and species diversity of aerobic organotrophic bacteria in the dagan high-temperature oil field (china), which is exploited via flooding, have been studied. twenty-two strains of the most characteristic thermophilic and mesophilic aerobic organotrophic bacteria have been isolated from the oil stratum. it has been found that, in a laboratory, the mesophilic and thermophilic isolates grow in the temperature, ph, and salinity ranges characteristic of the injection well near-bottom ... | 2005 | 16119855 |
| crystallization and preliminary x-ray analysis of thermoactinomyces vulgaris r-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylase. | a maltooligosaccharide-metabolizing enzyme from thermoactinomyces vulgaris r-47 (tga) homologous to glucoamylase degrades maltooligosaccharides more efficiently than starch, unlike fungal glucoamylases. tga was crystallized and the state of the protein in solution was analyzed by gel-filtration chromatography. diffraction data were collected to 3.31 a resolution. the tga crystal belongs to the orthorhombic space group p2(1)2(1)2(1) or p2(1)2(1)2, with unit-cell parameters a = 110.2, b = 317.6, c ... | 2005 | 16511025 |
| complex structures of thermoactinomyces vulgaris r-47 alpha-amylase 1 with malto-oligosaccharides demonstrate the role of domain n acting as a starch-binding domain. | the x-ray structures of complexes of thermoactinomyces vulgaris r-47 alpha-amylase 1 (tvai) with an inhibitor acarbose and an inactive mutant tvai with malto-hexaose and malto-tridecaose have been determined at 2.6, 2.0 and 1.8a resolution, and the structures have been refined to r-factors of 0.185 (r(free)=0.225), 0.184 (0.217) and 0.164 (0.200), respectively, with good chemical geometries. acarbose binds to the catalytic site of tvai, and interactions between acarbose and the enzyme are very s ... | 2004 | 14687576 |
| purification, characterization, and subsite affinities of thermoactinomyces vulgaris r-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylases. | a maltooligosaccharide-metabolizing enzyme from thermoactinomyces vulgaris r-47 (tga) homologous to glucoamylases does not degrade starch efficiently unlike most glucoamylases such as fungal glucoamylases (uotsu-tomita et al., appl. microbiol. biotechnol., 56, 465-473 (2001)). in this study, we purified and characterized tga, and determined the subsite affinities of the enzyme. the optimal ph and temperature of the enzyme are 6.8 and 60 degrees c, respectively. activity assays with 0.4% substrat ... | 2004 | 14981306 |
| reconstruction of bacillus thuringiensis ssp. israelensis cry11a endotoxin from fragments corresponding to its n- and c-moieties restores its original biological activity. | subtilisin hydrolyzes cry11a endotoxin (of 70 kd) produced by bacillus thuringiensis ssp. israelensis to fragments of 33- and 36-kd, which correspond to n- and c-terminal halves of the endotoxin molecule. thermitase (a serine protease from thermoactinomyces vulgaris) and insect gut proteases from diptera and lepidoptera exhibit the same hydrolytic effect on cry11a. hydrolyzates maintain high toxicity with respect to larvae of aedes aegypti, anopheles stephensi, and culex pipiens. the 33- and 36- ... | 2004 | 15000685 |
| [a case of farmer's lung disease manifested by smoking cessation]. | a 40-year-old man, a cattle hoof-chipper, was admitted to hokkaido university hospital in june 2002 for a workup of the occasional fever, cough and dyspnea that had affected him at work since march 2002. he had not smoked since january 2001. on admission, he had a marked increase in the proportion of lymphocytes (62.9%) and the cd 4/cd 8 ratio (3.0) in the bronchoalveolar lavage fluid. serum precipitating antibody against thermoactinomyces vulgaris was detected. the results of a transbronchial l ... | 2004 | 15114848 |
| complex structures of thermoactinomyces vulgaris r-47 alpha-amylase 2 with acarbose and cyclodextrins demonstrate the multiple substrate recognition mechanism. | thermoactinomyces vulgaris r-47 alpha-amylase 2 (tvaii) has the unique ability to hydrolyze cyclodextrins (cds), with various sized cavities, as well as starch. to understand the relationship between structure and substrate specificity, x-ray structures of a tvaii-acarbose complex and inactive mutant tvaii (d325n/d421n)/alpha-, beta- and gamma-cds complexes were determined at resolutions of 2.9, 2.9, 2.8, and 3.1 a, respectively. in all complexes, the interactions between ligands and enzymes at ... | 2004 | 15138257 |
| the crystal structure of thermoactinomyces vulgaris r-47 alpha-amylase ii (tva ii) complexed with transglycosylated product. | alphan alpha-amylase (tva ii) from thermoactinomyces vulgaris r-47 efficiently hydrolyzes alpha-1,4-glucosidic linkages of pullulan to produce panose in addition to hydrolyzing starch. tva ii also hydrolyzes alpha-1,4-glucosidic linkages of cyclodextrins and alpha-1,6-glucosidic linkages of isopanose. to clarify the basis for this wide substrate specificity of tva ii, we soaked 4(3)-alpha-panosylpanose (4(3)-p2) (a pullulan hydrolysate composed of two panosyl units) into crystals of d325n inacti ... | 2004 | 15182368 |
| ca2+ dependence and inhibitory effects of trifluoperazine on plasma membrane atpase of thermoactinomyces vulgaris. | ca2+ enhanced the plasma membrane ca(2+)-atpase (pmca) specific activities in wild-type strain 1227 and mutant strains 1278, 1286, and 1261 of thermoactinomyces vulgaris. the ca(2+)-atpase specific activities showed marked increase with increasing concentrations of ca2+ added in the form of cacl2 in the culture medium and reached the optimum values at 0.6 mm in strains 1227, 1278, and 1286 and at 0.7 mm in strain 1261 of t. vulgaris. trifluoperazine, a specific blocker of calmodulin, when added ... | 2004 | 15297926 |
| a novel neutral protease from thermoactinomyces species 27a: sequencing of the gene, purification, and characterization of the enzyme. | the nucleotide sequence of the previously cloned (zabolotskaya, m. v., nosovskaya, e. a., kaplun, m. a., and akimkina, t. v. (2001). mol. gen. mikrobiol. virusol. no 1, 32-34) dna fragment from thermoactinomyces sp. 27a (genbank accession no. ay280367) containing the metalloproteinase gene was determined. a continuous open reading frame encoding a polypeptide of 673 aa was revealed. analysis of this sequence demonstrated that the metalloproteinase from thermoactinomyces sp. 27a is synthesized as ... | 2004 | 15635941 |
| exposure to bioaerosols in a municipal sewage treatment plant. | microbiological air sampling was performed in a medium-size sewage treatment plant processing municipal wastewater from a city located in eastern poland. air samples for determination of the concentrations of viable mesophilic bacteria, gram-negative bacteria, thermophilic actinomycetes, fungi and endotoxin were collected at 12 sites associated with various phases of sewage treatment process. the concentrations of total mesophilic bacteria (both gram-positive and gram-negative) were within a ran ... | 2003 | 14677919 |
| fluorescence based rrna sensor systems for detection of whole cells of saccharomonospora spp. and thermoactinomyces spp. | airborne thermophilic actinomycetes (tpas) are a growing hygienic challenge in different occupational situations e.g. large scale composting. this study describes first results of a new approach for highly specific and rapid detection of organisms of this group using fluorescently labelled oligonucleotide probes as sensors for whole cells. three genus-specific 16s rrna-targeted probes, two for saccharomonospora spp. and one for thermoactinomyces spp. were developed and evaluated in a fluorescenc ... | 2003 | 12706563 |
| ca2+ -dependence and inhibition of transformation by trifluoperazine and chlorpromazine in thermoactinomyces vulgaris. | ca(2+) enhanced the transformation frequency of thermoactinomyces vulgaris (stock no. 1278) of an auxotrophic strain by the chromosomal dna isolated from a prototrophic strain (stock no. 1227). the number of transformants showed a marked increase with increasing concentration of cacl(2) upto 0.05 mm; and above this concentration, the transformation frequency decreased significantly. antipsychotic drugs that are potent calmodulin inhibitors, like trifluoperazine and chlorpromazine, when applied i ... | 2003 | 12732975 |
| mutual conversion of substrate specificities of thermoactinomyces vulgaris r-47 alpha-amylases tvai and tvaii by site-directed mutagenesis. | thermoactinomyces vulgaris r-47 produces two alpha-amylases, tvai and tvaii, differing in substrate specificity from each other. tvai favors high-molecular-weight substrates like starch, and scarcely hydrolyzes cyclomaltooligosaccharides (cyclodextrins) with a small cavity. tvaii favors low-molecular-weight substrates like oligosaccharides, and can efficiently hydrolyze cyclodextrins with various sized cavities. to understand the relationship between the structure and substrate specificity of th ... | 2003 | 12860426 |
| [the appearance of the thermophilic actinomycetes in dairy products]. | research covered testing of 30 samples of the following milk products: cream, sweet cream, fat cottage cheese, semi-fat cottage cheese, low-fat cottage cheese, homogenized cheese and butter. the presence of thermophilic ray fungi was confirmed in all examinated samples of tested products. in particular milk derivates the following levels of contaminations were found: cottage cheese--27.3-33.5 cfu/g, homogenized cheese--21.7 cfu/g, cream--19.3 cfu/g, sweet cream--14.6 cfu/g and butter--11.0 cfu/g ... | 2003 | 12870300 |
| 3d structure of sulfolobus solfataricus carboxypeptidase developed by molecular modeling is confirmed by site-directed mutagenesis and small angle x-ray scattering. | sulfolobus solfataricus carboxypeptidase (cpsso) is a thermostable zinc-metalloenzyme with a m(r) of 43,000. taking into account the experimentally determined zinc content of one ion per subunit, we developed two alternative 3d models, starting from the available structures of thermoactinomyces vulgaris carboxypeptidase (model a) and pseudomonas carboxypeptidase g2 (model b). the former enzyme is monomeric and has one metal ion in the active site, while the latter is dimeric and has two bound zi ... | 2003 | 12885660 |
| identification and properties of type i-signal peptidases of bacillus amyloliquefaciens. | the use of bacillus amyloliquefaciens for enzyme production and its exceptional high protein export capacity initiated this study where the presence and function of multiple type i signal peptidase isoforms was investigated. in addition to type i signal peptidases sips(ba) [meijer, w.j.j., de jong, a., bea, g., wisman, a., tjalsma, h., venema, g., bron, s. & van dijl, j.m. (1995) mol. microbiol. 17, 621-631] and sipt(ba) [hoang, v. & hofemeister, j. (1995) biochim. biophys. acta 1269, 64-68] whi ... | 2002 | 11856304 |
| crystallization and preliminary x-ray analysis of substrate complexes of leucine dehydrogenase from thermoactinomyces intermedius. | leucine dehydrogenase is an octameric enzyme which belongs to the superfamily of amino-acid dehydrogenases and catalyses the reversible oxidative deamination of leucine to 2-ketoisocaproate, with the corresponding reduction of the cofactor nad(+). catalysis by this enzyme is thought to involve a large-scale motion of the enzyme's two domains between an 'open' and 'closed' form, with the latter representing a conformation of the enzyme in which the partners involved in the hydride-transfer reacti ... | 2002 | 12037317 |
| crystal structures and structural comparison of thermoactinomyces vulgaris r-47 alpha-amylase 1 (tvai) at 1.6 a resolution and alpha-amylase 2 (tvaii) at 2.3 a resolution. | the x-ray crystal structures of thermoactinomyces vulgaris r-47 alpha-amylase 1 (tvai) and alpha-amylase 2 (tvaii) have been determined at 1.6 a and 2.3 a resolution, respectively. the structures of tvai and tvaii have been refined, r-factor of 0.182 (r(free)=0.206) and 0.179 (0.224), respectively, with good chemical geometries. both tvai and tvaii have four domains, n, a, b and c, and all very similar in structure. however, there are some differences in the structures between them. domain n of ... | 2002 | 12051850 |
| improved molecular identification of thermoactinomyces spp. associated with mushroom worker's lung by 16s rdna sequence typing. | mushroom worker's lung (mwl) is a hypersensitivity pneumonitis or allergic alveolitis caused by a type iii igg-mediated immunopathogenic inflammatory reaction in the host due to the inhalation of several thermophilic organisms, including thermoactinomyces spp. it is difficult to distinguish phenotypically the eight species of this genus; therefore, this study sought to develop an improved molecular means of identifying thermoactinomyces spp. associated with mwl by partial 16s rdna pcr amplificat ... | 2002 | 12466412 |
| precipitin response of potato processing workers to work-related microbial allergens. | serum samples from 61 potato processing workers and 30 urban dwellers not exposed to organic dusts (as a reference group) were examined in agar-gel precipitation test performed by ouchterlony double diffusion method with the antigens of 12 microorganisms associated with organic dusts. each serum was tested twice: not concentrated, and three-fold concentrated, for the detection of low levels of precipitins. the antibody response of workers to the antigen of coryneform bacterium agromyces ramosus ... | 2002 | 12498593 |
| [characterization of s1' subsite specificity of thermoactinomyces vulgaris carboxypeptidase t by site-directed mutagenesis]. | the site-directed mutagenesis in the s1'-pocket of thermoactinomyces vulgaris carboxypeptidase t was performed and two variants containing double d253s, t255d and single t255d mutations were obtained. precursors of the wild-type carboxypeptidase t and its mutant derivatives were expressed in escherichia coli as inclusion bodies, refolded, activated by subtilisin, purified by gel efiltration on superdex g-75. the catalytic activity with tripeptide substrates dnpaar and zaal was analysed. the intr ... | 2002 | 12698557 |
| bioactive rare actinomycetes from indigenous natural substrates of andhra pradesh. | this investigation reports the occurrence of bioactive rare actinomycetes from different indigenous natural substrates of andhra pradesh. during the course of our investigation on 20 terrestrial soil samples, 5 marine samples and 3 fresh water samples, a total of 92 rare actinomycetes belonging to micromonospora, nocardia, actinomadura and thermoactinomyces genera were isolated. the antimicrobial and enzymatic activities were studied for all the isolates. the antibacterial and antifungal activit ... | 2002 | 15061589 |
| studies on the hydrolyzing mechanism for cyclodextrins of thermoactinomyces vulgaris r-47 alpha-amylase 2 (tvaii). x-ray structure of the mutant e354a complexed with beta-cyclodextrin, and kinetic analyses on cyclodextrins. | crystals of the mutant e354a of thermoactinomyces vulgaris r-47 alpha-amylase 2 (tvaii) complexed with beta-cyclodextrin were prepared by a soaking method, and the diffraction data were collected at 100 k, using synchrotron radiation (spring-8). the crystals belong to an orthorhombic system with the space group p2(1)2(1)2(1) and cell dimensions a = 111.1 a, b = 117.7 a, c = 113.3 a, which is almost isomorphous with crystals of the wild-type tvaii, and the structure was refined to an r-factor = 0 ... | 2001 | 11226882 |
| [a new thermostable proteinase from thermoactinomyces sp.27a. cloning and gene expression]. | a library of thermoactinomyces sp. 27a, producer of thermostable proteases of different groups, has been created. gene coding for thermostable neutral proteinase was cloned and expressed in bac. subtilis cells. restriction map for cloned dna fragment was created and physicochemical parameters of recombinant proteinase were characterized. the thermostability and optimum of proteolytic activity of the enzyme was lower than in the natural thermoactinomyces sp. strain, which can be due to heterologo ... | 2001 | 11236740 |
| the deletion of amino-terminal domain in thermoactinomyces vulgaris r-47 alpha-amylases: effects of domain n on activity, specificity, stability and dimerization. | thermoactinomyces vulgaris r-47 alpha-amylases, tva i and tva ii, have a domain n, which is an extra structure in the family 13 enzymes. to investigate the roles of domain n in tvas, we constructed tvas-deltan mutants which are deleted in domain n, and y14,16,68a and y41,82,95a mutants of tva ii. tvas-deltan were unstable under alkaline conditions, and their thermal stabilities were 10 degrees c lower than that of wild-types. the specific activities of tvas-deltan for pullulan, starch, cyclodext ... | 2001 | 11302176 |
| structures of thermoactinomyces vulgaris r-47 alpha-amylase ii complexed with substrate analogues. | the structures of thermoactinomyces vulgaris r-47 alpha-amylase ii mutant (d325ntva ii) complexed with substrate analogues, methyl beta-cyclodextrin (m beta-cd) and maltohexaose (g6), were solved by x-ray diffraction at 3.2 a and 3.3 a resolution, respectively. in d325ntva ii-m beta-cd complex, the orientation and binding-position of beta-cd in tva ii were identical to those in cyclodextin glucanotransferase (cgtase). the active site residues were essentialy conserved, while there are no residue ... | 2001 | 11330677 |
| thermostable, alkalophilic and cellulase free xylanase production by thermoactinomyces thalophilus subgroup c. | thermoactinomyces thalophilus produced cellulase free extracellular endo-1,4-beta-xylanase (ec 3.2.1.8) at 50 degrees c and ph 8.5. maximum xylanase production was achieved in fermentation medium using birchwood xylan as substrate after 96 h of growth at 50 degrees c. other agricultural substrates such as wheat bran, wheat straw, sugarcane bagasse and cornstover produced less xylanase. the crude enzyme preparation from mutant t. thalophilus p2 grown under optimised fermentation conditions showed ... | 2001 | 11339942 |
| phylogenetic diversity of thermophilic actinomycetes and thermoactinomyces spp. isolated from mushroom composts in korea based on 16s rrna gene sequence analysis. | forty one strains isolated from 21 samples of various mushroom composts in korea were analyzed by 16s rrna gene sequencing to investigate the phylogenetic diversity of thermophilic actinomycetes. the 25 strains of thermophilic actinomycete isolates were related to the five genera, pseudonocardia, saccharomonospora, saccharopolyspora, streptomyces, and thermobifida, within the order actinomycetales, and 16 strains were classified into the genus thermoactinomyces within the family bacillaceae. mos ... | 2001 | 11506914 |
| random amplified ribosomal dna restriction analysis for rapid identification of thermophilic actinomycete-like bacteria involved in hypersensitivity pneumonitis. | hypersensitivity pneumonitis (hp) is a pulmonary disease characterised by inflammation that can be caused by, amongst other substances, a subset of 4 thermophilic mycelial bacteria: saccharopolyspora rectivirgula, saccharomonospora viridis, thermoactinomyces sacchari, and thermoactinomyces vulgaris. air sampling analyses in highly contaminated environments are often performed to evaluate exposure to these species which are difficult and fastidious to identify by conventional techniques. the aim ... | 2001 | 11518332 |
| role of phe286 in the recognition mechanism of cyclomaltooligosaccharides (cyclodextrins) by thermoactinomyces vulgaris r-47 alpha-amylase 2 (tvaii). x-ray structures of the mutant tvaiis, f286a and f286y, and kinetic analyses of the phe286-replaced mutant tvaiis. | phe286 located in the center of the active site of alpha-amylase 2 from thermoactinomyces vulgaris r-47 (tvaii) plays an important role in the substrate recognition for cyclomaltooligosaccharides (cyclodextrins). the x-ray structures of mutant tvaiis with the replacement of phe286 by ala (f286a) and tyr (f286y) were determined at 3.2 a resolution. their structures have no significant differences from that of the wild-type enzyme. the kinetic analyses of phe286-replaced variants showed that the v ... | 2001 | 11527532 |
| novel glucoamylase-type enzymes from thermoactinomyces vulgaris and methanococcus jannaschii whose genes are found in the flanking region of the alpha-amylase genes. | a region downstream of the gene for pullulan-hydrolyzing alpha-amylase, tva ii, of thermoactinomyces vulgaris r-47 was sequenced, and an open reading frame encoding an enzyme homologous to glucoamylase was found. the nucleotide sequence of this enzyme, designated tga, consists of 1,953 base pairs corresponding to a protein of 651 amino acid residues. the tga gene was subcloned and expressed in escherichia coli. enzymatic analyses showed that, like other glucoamylases, tga produced beta-d-glucose ... | 2001 | 11549021 |
| physiology, biochemistry and taxonomy of deep-sea nitrile metabolising rhodococcus strains. | a collection of nitrile-hydrolysing rhodococci was isolated from sediments sampled from a range of deep coastal, and abyssal and hadal trench sites in the nw pacific ocean, as part of our programme on the diversity of marine actinomycetes. nitrile-hydrolysing strains were obtained by batch enrichments on nitrile substrates with or without dispersion and differential centrifugation pre-treatment of sediments, and were recovered from all of the depths sampled (approximately 1100-6500 m). two isola ... | 2001 | 11759050 |
| synthesis of allysine ethylene acetal using phenylalanine dehydrogenase from thermoactinomyces intermedius. | allysine ethylene acetal [(s)-2-amino-5-(1,3-dioxolan-2-yl)-pentanoic acid (2)] was prepared from the corresponding keto acid by reductive amination using phenylalanine dehydrogenase (pdh) from thermoactinomyces intermedius atcc 33205. glutamate, alanine, and leucine dehydrogenases, and pdh from sporosarcina species (listed in order of increasing effectiveness) also gave the desired amino acid but were less effective. the reaction requires ammonia and nadh. nad produced during the reaction was r ... | 2000 | 10713207 |
| structure, specificity and function of cyclomaltodextrinase, a multispecific enzyme of the alpha-amylase family. | cyclomaltodextrinase (cdase, ec 3.2.1.54), maltogenic amylase (ec 3. 2.1.133), and neopullulanase (ec 3.2.1.135) are reported to be capable of hydrolyzing all or two of the following three types of substrates: cyclomaltodextrins (cds); pullulan; and starch. these enzymes hydrolyze cds and starch to maltose and pullulan to panose by cleavage of alpha-1,4 glycosidic bonds whereas alpha-amylases essentially lack activity on cds and pullulan. they also catalyze transglycosylation of oligosaccharides ... | 2000 | 10825529 |
| phylogenetic analysis of the genus thermoactinomyces based on 16s rdna sequences. | the almost complete 16s rdna sequences of the type strains of eight validly described species and two invalid species of the genus thermoactinomyces were determined and phylogenetically analysed. the validly described thermoactinomyces species formed phylogenetic lineages related to the family bacillaceae, as shown previously. however, the available strains of 'thermoactinomyces glaucus' and 'thermoactinomyces monosporus' exhibited their closest phylogenetic affinities not to the genus thermoact ... | 2000 | 10843048 |
| production of cutinolytic esterase by filamentous bacteria. | thirty-eight strains of filamentous bacteria, many of which are thermophilic or thermotolerant and commonly found in composts and mouldy fodders, were examined for their ability to produce cutinolytic esterase (cutinase) in culture media supplemented with cutin, suberin or cutin-containing agricultural by-products. initially, the ability of culture supernatants to hydrolyse the artificial substrate p-nitrophenyl butyrate was determined by spectrophotometric assays. only one bacterium, thermoacti ... | 2000 | 10886609 |
| specificity of a neutral zn-dependent proteinase from thermoactinomyces sacchari toward the oxidized insulin b chain. | the proteolytic specificity of the neutral zn-dependent proteinase from thermoactinomyces sacchari was determined by analysis of the peptides obtained after incubation with the oxidized insulin b chain as a substrate. the enzyme is an endopeptidase with broad specificity. in total, 12 peptide bonds in the b chain of insulin were hydrolyzed. the major requirement is that a hydrophobic residue such as leu, val, or phe should participate with the alpha-amino group in the bond to be cleaved. however ... | 2000 | 10919403 |
| serum kl-6 concentrations in dairy farmers. | serum kl-6 (krebs von den lungen-6) has been recognized to be a marker for the activity of diffuse interstitial lung diseases. the purpose of the study is to evaluate serum kl-6 measurement as a marker for farmer's lung disease (fld). | 2000 | 10936139 |
| dna-dna relatedness among thermoactinomyces species: thermoactinomyces candidus as a synonym of thermoactinomyces vulgaris and thermoactinomyces thalpophilus as a synonym of thermoactinomyces sacchari. | dna-dna relatedness of all validly described thermoactinomyces species was determined to infer the genetic relationships between them. the levels of dna-dna relatedness among the type strains of thermoactinomyces species ranged from 2-5 to 92.8%. based on dna relatedness data, the type strains of thermoactinomyces intermedius, thermoactinomyces putidus, thermoactinomyces dichotomicus and thermoactinomyces peptonophilus were considered to be distinct species of the genus thermoactinomyces. howeve ... | 2000 | 11034503 |
| cloning and expression of the ccpa gene encoding catabolite control protein from thermoactinomyces sp. e79. | the gene ccpa encoding the catabolite control protein a (ccpa) of thermoactinomyces sp. e79 has been cloned and characterized. nucleotide sequence analysis of the ccpa clone showed that the cloned fragment contained the full structural gene for a protein of 346 amino acids. the predicted amino acid sequence shows similarity to the transcriptional regulators of the lacl-galr family; a highly conserved helix-turn-helix motif, which might bind to dna, was identified through comparison with regulato ... | 2000 | 11129608 |
| analysis of catalytic residues of thermoactinomyces vulgaris r-47 alpha-amylase ii (tva ii) by site-directed mutagenesis. | to confirm that the catalytic residues (asp325, glu354, and asp421) are necessary for the hydrolysis of starch, pullulan, and cyclodextrins, we constructed tva ii mutated by site-directed mutagenesis. the mutated enzymes (d325n, e354q, and d421n) had markedly reduced levels of activity, less than 0.006% of the wild type, indicating that these three residues are the catalytic sites for these substrates. even e354d had reduced levels of activity, less than 0.05% of wild type. these four mutated en ... | 2000 | 11210138 |
| transfer of 'thermoactinomyces glaucus' ifo 12530 and 'thermoactinomyces monosporus' ifo 14050 to the genus saccharomonospora as members of saccharomonospora glauca. | two available strains of 'thermoactinomyces glaucus' and 'thermoactinomyces monosporus', 't. glaucus' ifo 12530 and 't. monosporus' ifo 14050, were considered not to be members of the genus thermoactinomyces and that they belonged to the genus saccharomonospora on the basis of the colors of colonies and 16s rdna sequences. some chemotaxonomic characteristics also showed that the two strains belong to the genus saccharomonospora. the two strains contained meso-diaminopimelic acid, galactose, and ... | 2000 | 12483576 |
| directed evolution converts subtilisin e into a functional equivalent of thermitase. | we used directed evolution to convert bacillus subtilis subtilisin e into an enzyme functionally equivalent to its thermophilic homolog thermitase from thermoactinomyces vulgaris. five generations of random mutagenesis, recombination and screening created subtilisin e 5-3h5, whose half-life at 83 degrees c (3.5 min) and temperature optimum for activity (topt, 76 degrees c) are identical with those of thermitase. the topt of the evolved enzyme is 17 degrees c higher and its half-life at 65 degree ... | 1999 | 10065710 |
| [cloning and expression of a serine proteinase gene from thermoactinomyces sp. in bacillus subtilis cells]. | a gene coding for thermostable serine protease from thermoactinomyces sp. k50 is cloned and expressed in bacillus subtilis cells. restriction map of cloned dna fragment is determined. thermostability and temperature optimum of proteolytic activity of the cloned gene product are lower than those of the natural proteinase of thermoactinomyces sp. k50. serine protease, a product of cloned gene, is highly sensitive to proteolysis and its degradation can be prevented by ca2+ ions. | 1999 | 10190105 |
| crystal structure of thermoactinomyces vulgaris r-47 alpha-amylase ii (tvaii) hydrolyzing cyclodextrins and pullulan at 2.6 a resolution. | the crystal structure of thermoactinomyces vulgaris r-47 alpha-amylase ii (tvaii) has been determined by multiple isomorphous replacement at 2.6 a resolution. tvaii was crystallized in an orthorhombic system with the space group p212121 and the cell dimensions a=118.5 a, b=119.5 a, c=114.5 a. there are two molecules in an asymmetric unit, related by the non-crystallographic 2-fold symmetry. diffraction data were collected at 113 k and the cell dimensions reduced to a=114.6 a, b=117.9 a, c=114.2 ... | 1999 | 10222200 |
| isolation and partial characterisation of extracellular keratinase from a wool degrading thermophilic actinomycete strain thermoactinomyces candidus. | the keratinase production by the thermophilic actinomycete strain thermoactinomyces candidus was induced by sheep wool as the sole source of carbon and nitrogen in the cultivation medium. for complete digestion of wool by the above strain, both keratinolytic serine proteinase and cellular reduction of disulfide bonds were involved. evidence was presented that substrate induction was a major regulatory mechanism and the keratinase biosynthesis was not completely repressed by addition of other car ... | 1999 | 10408094 |
| igg antibodies, chronic bronchitis, and pulmonary function values in farmer's lung patients and matched controls. | we measured igg antibody levels against eight different microbes in farmer's lung (fl) patients an average of 14 years after the first diagnosed episode of fl and in matched controls. the study population consisted of 87 fl patients and 81 control farmers, matched by age, sex, and smoking habits. clinical studies included the measurement of igg antibody levels against absidia corymbifera, aspergillus umbrosus, a. fumigatus, humicola grisea, saccharopolyspora rectivirgula, penicillium brevicompac ... | 1999 | 10604554 |
| novel rhodococci and other mycolate actinomycetes from the deep sea. | a large number of mycolate actinomycetes have been recovered from deep-sea sediments in the nw pacific ocean using selective isolation methods. the isolates were putatively assigned to the genus rhodococcus on the basis of colony characteristics and mycolic acid profiles. the diversity among these isolates and their relationship to type strains of rhodococcus and other mycolate taxa were assessed by curie point pyrolysis mass spectrometry (pyms). three major (a, c, d) and two minor (b, e) groups ... | 1998 | 10068786 |
| effect of inhalation of organic dust-derived microbial agents on the pulmonary phagocytic oxidative metabolism of guinea pigs. | the effect of inhalation exposure of various biological agents associated with organic dusts on the function of guinea pigs pulmonary phagocytes was investigated. agents included antigens of erwinia herbicola, thermoactinomyces vulgaris, and aspergillus fumigatus; endotoxin of erwinia herbicola; bacterial protease; and a fungal glucan preparation. pulmonary parameters monitored in this study were cellular differential counts from bronchoalveolar lavage, and superoxide anion and/or hydrogen perox ... | 1998 | 9447225 |
| expression of the carboxypeptidase t gene from thermoactinomyces vulgaris in stable protoplast type l-forms of proteus mirabilis. | the structural gene of the carboxypeptidase t (cpt) was successfully expressed in cell wall-less l-form cells of proteus mirabilis. the dna sequence encoding the phoa leader peptide was fused with a truncated cpt gene encoding the mature enzyme. the modified gene in a puc-based kanamycin resistance vector under the control of the lac promoter was transformed into l-form cells of p. mirabilis. they were able to produce the recombinant cpt both as a secretory and as a cell-bound insoluble form. th ... | 1998 | 9503606 |
| a cysteine-dependent serine protease associated with the dormant spores of bacillus cereus: purification of the protein and cloning of the corresponding gene. | subtilisin-like serine protease, which is associated with the dormant spores of bacillus cereus, was solubilized by washing the spores with 2 m kcl and purified to homogeneity by carbobenzoxy-d-phenylalanine-liganded affinity column chromatography and hydrophobic interaction column chromatography. enzyme activity was completely inhibited by reagents for sulfhydryl groups such as hgcl2 as well as by conventional subtilisin inhibitors, suggesting the enzyme to be cysteine-dependent. the enzyme ret ... | 1998 | 9532782 |
| conversion of neopullulanase-alpha-amylase from thermoactinomyces vulgaris r-47 into an amylopullulanse-type enzyme. | tva i, an alpha-amylase from thermoactinomyces vulgaris r-47, is a versatile enzyme which hydrolyzes the alpha-(1-->4)-glucosidic linkages of pullulan to produce panose, known as neopullulanase activity, and the alpha-(1-->6)-glucosidic linkages of certain oligosaccharides. we modified the ala-357, gln-359, and tyr-360 residues located in region ii, one of the four regions conserved in alpha-amylase family enzymes, and deleted 11 consecutive amino acid residues located after the c-terminus of re ... | 1998 | 9538203 |
| characteristics of airborne actinomycete spores. | airborne actinomycete spores, important contaminants in occupational and residential environments, were studied with respect to their (i) release into the air, (ii) aerodynamic and physical size while airborne, and (iii) survival after collection onto agar with an impactor. three actinomycete species were selected for the tests to exemplify the three main spore types: streptomyces albus for arthrospores, micromonospora halophytica for aleuriospores, and thermoactinomyces vulgaris for endospores. ... | 1998 | 9758803 |
| analysis of airborne actinomycete spores with fluorogenic substrates. | the reactions between seven fluorogenic substrates and different groups of enzymes, esterases, lipases, phosphatases, and dehydrogenases, were studied in a search for a new method for the detection of actinomycete spores. fluorescence measurement was chosen as a fast and sensitive method for microbial analysis. the focus of the research was on the spores of important air contaminants: streptomyces albus and thermoactinomyces vulgaris. for the measurement of the enzymatic activity, the chosen flu ... | 1998 | 9797299 |
| construction of a new leucine dehydrogenase with preferred specificity for nadp+ by site-directed mutagenesis of the strictly nad+-specific enzyme. | on the basis of sequence comparison between nad+-dependent leucine dehydrogenase (leudh) from thermoactinomyces intermedius and nadp+-dependent dehydrogenases, a set of amino acid residues that are supposed to determine the coenzyme specificity of leudh were assigned. systematic replacement of these amino acids by others was done with the aim to switch its natural coenzyme specificity to a new one preferring nadp+. single d203a, double d203a-i204r and triple d203a-i204r-d210r mutation enzymes we ... | 1997 | 9278282 |
| isolation and growth physiology of novel thermoactinomycetes. | a total of 34 thermophilic isolates identified as members of the genus thermoactinomyces by a range of chemotaxonomic, microscopic and determinative biochemical tests, were isolated from two acid soils. growth studies in shake flask and fermenter identified the isolates to be moderately acidophilic with growth occurring between ph 4.5 and 6.0 with optima at ph 5.0. the isolates differed considerably from known thermoactinomyces cultures in their ph profile, colony morphology and in several bioch ... | 1997 | 9449806 |
| cloning and expression of an intracellular alkaline protease gene from alkalophilic thermoactinomyces sp. hs682. | an intracellular alkaline serine protease gene of alkalophilic thermoactinomyces sp. hs682 was cloned and expressed in escherichia coli. sequence analysis showed a putative promoter region, a putative transcriptional termination signal, and an open reading frame of 963 bases, coding for a polypeptide of 321 amino acids. the protease expressed in e. coli was purified by deae-toyopearl 650m and sephadex g-75 chromatography. the n-terminal sequence (30 amino acids) of the purified protein was coinc ... | 1997 | 9058969 |
| purification and characterization of serine proteinase of the glu,asp-specific enzyme family from thermoactinomyces species. | enzyme catalyzing hydrolysis of a substrate of glu,asp-specific proteinases (z-glu-pna) and cleaving bond glu13-ala14 in the oxidized insulin b chain was purified to homogeneity from the culture medium of thermoactinomyces species using hydrophobic chromatography on phenyl-sepharose cl 4b as the key purification step. the molecular weight of the proteinase is 23 kd. the enzyme is completely inhibited by diisopropyl fluorophosphate and is stable at ph 5-11. the ph optimum for the hydrolysis of az ... | 1997 | 9159871 |
| cloning, sequencing and overexpression of the leucine dehydrogenase gene from bacillus cereus. | the l-leucine dehydrogenase gene from bacillus cereus (dsm 626) was cloned from a partial genomic library and sequenced. the open reading frame has 1101 bp and codes for a protein of 39.9 kda. the deduced amino acid sequence of the leudh from b. cereus shares 70-80% identity with leudh's from the thermophilic strains b. stearothermophilus and thermoactinomyces intermedius. the active protein was overexpressed in escherichia coli to yield approximately 30% of the total soluble protein. | 1997 | 9188201 |
| [the influence of organic dust on the chemotaxis of lung cells. experimental studies]. | mechanisms of chemotaxis of alveolar macrophages (ams) and neutrophils (pmns) in response to microbial products derived from organic dust were studied using blindwell chemotaxis chamber technique. seven different agents (extract and endotoxin of erwinia herbicola, extracts from thermoactinomyces vulgaris and aspergillus fumigatus, thermophilic protease and two preparations of glucans), were used for experiments. these agents were evaluated for their ability to direct attraction of ams and pmns a ... | 1996 | 9190243 |
| microflora and acarofauna of bed dust from homes in upper silesia, poland. | pyroglyphid mites are considered a major cause of house dust allergy. the occurrence and possible pathogenic role of other biologic components of house dust, in particular bacteria, has received less attention. | 1996 | 8564627 |
| purification and characterization of a thermostable alkaline protease from thermoactinomyces sp. e79 and the dna sequence of the encoding gene. | a thermophilic thermoactinomyces sp. e79 producing a highly thermostable alkaline protease was isolated from soil. the protease, produced extracellularly by thermoactinomyces sp. e79, was purified by deae-sepharose cl-6b and butyl-toyopearl 650m column chromatography. the relative molecular mass was estimated to be 31,000 by sds-polyacrylamide gel electrophoresis. enzyme activity was inhibited by phenylmethylsulfonyl fluoride, suggesting the enzyme to be a serine protease. the optimum temperatur ... | 1996 | 8704314 |
| [indoor air studies of mould fungus contamination of homes of selected patients with bronchial asthma (with special regard to evaluation problems)]. | investigations of indoor air of the homes of seven patients with asthma bronchiale who showed up with positive reactions following intracutaneous application of fungal allergens revealed that their places of residence were contaminated by fungal and bacterial spores. the number of colony forming units of mesophilic fungal spores of the indoor air ranged from 100 to 1000 cfu/m3 and this was much higher than the mould flora of the outdoor air determined simultaneously. the major fungi species foun ... | 1996 | 9376049 |
| thermitase - kinetic differentiation to the subtilisins. | in the last 12 years more than 80 peptidyl substrates and substrate analogous inhibitors were synthesized in our group and used to characterize thermitase, a serine proteinase of the subtilisin family produced by thermoactinomyces vulgaris. the kinetic parameters of hydrolysis and inhibition reactions, respectively, with thermitase were determined and compared with those found with related enzymes. conclusions with respect to the extent of the active site of thermitase and to the specificity of ... | 1996 | 8796317 |
| the medical and epidemiologic effects on workers of the levels of airborne thermoactinomyces spp. spores present in australian raw sugar mills. | the objectives of the study were to determine whether there was a significant risk of members of the work force of raw sugar mills developing bagassosis. airborne thermoactinomyces sacchari spores were measured to determine whether they were sufficient to cause acute bagassosis, and whether there was any evidence of previous exposure to sufficient airborne t. sacchari spores to cause the development of chronic bagassosis in any of the work force. monitoring of total airborne bacteria spore conce ... | 1996 | 8931309 |