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characterization of cimex lectularius (bedbug) defensin peptide and its antimicrobial activity against human skin microflora.antimicrobial peptides are components of both vertebrate and invertebrate innate immune systems that are expressed in response to exposure to bacterial antigens. naturally occurring antimicrobial peptides from evolutionarily ancient species have been extensively studied and are being developed as potential therapeutics against antibiotic resistant microorganisms. in this study, a putative cimex lectularius (bedbug, cl) defensin is characterized for its effectiveness against human skin flora incl ...201626802465
diphtheroids-important nosocomial pathogens.diphtheroids are defined as aerobic, non-sporulating, pleomorphic gram-positive bacilli which are more uniformly stained than corynebacterium diphtheriae, lack the metachromatic granules and are arranged in a palisade manner. they are usually commensals of the skin and mucous membranes. they differ from c.diphtheriae in biochemical rea-ctions as well as in toxin production. since, they are usually found as commensals on the skin, they are often considered as mere contaminants when isolated from ...201628208859
draft genome sequences of two pathogenic corynebacterial species isolated from cows.the species corynebacterium renale, corynebacterium pilosum, and corynebacterium cystitidis were initially thought to be the same species c. renale, but with different immunological types. these bacteria are the causative agent of cystitis, urethritis and pyelonephritis and are found usually as constituents of the normal flora in the lower urogenital tract of cattle. therefore, we present the draft genome sequences of two pathogenic corynebacterium species: c. renale cip 52.96 and c. pilosum cip ...201626958092
two rep genes in small cryptic plasmid pkst21 of escherichia coli.the complete nucleotide sequence of a small cryptic plasmid pkst21 from escherichia coli was determined. this plasmid is 1,460 bp long with an overall gc content of 51 %. based on sequence analysis, the presence of two segments with different average gc density was observed. the segment with higher gc content revealed 98-90 % similarity to several small plasmids of e. coli and to pcr1 from gram-positive corynebacterium renale. plasmid pkst21 possesses two conversely oriented open reading frames ...201323680975
characterization of corynebacterium species in macaques.bacteria of the genus corynebacterium are important primary and opportunistic pathogens. many are zoonotic agents. in this report, phenotypic (api coryne analysis), genetic (rpob and 16s rrna gene sequencing), and physical methods (ms) were used to distinguish the closely related diphtheroid species corynebacterium ulcerans and corynebacterium pseudotuberculosis, and to definitively diagnose corynebacterium renale from cephalic implants of rhesus (macaca mulatta) and cynomolgus (macaca fascicula ...201222723254
tertiary structure-related activity of tick defensin (persulcatusin) in the taiga tick, ixodes persulcatus.defensins are small cysteine-rich cationic proteins found in both vertebrates and invertebrates constituting the front line of host innate immunity. to examine the importance of the tertiary structure of tick defensin in its antimicrobial activity, we synthesized two types of the peptides with tertiary structure or primary one on basis of the information of the sequence in the defensin originated from the taiga tick, ixodes persulcatus. chemically synthesized peptides were used to investigate th ...201120596886
physiological and biochemical consequences of host-plasmid interaction--a case study with corynebacterium renale, a multiple cryptic plasmid containing strain.corynebacterium renale harbors four small cryptic plasmids, pcr1, pcr2, pcr3 and pcr4, and can be a good system for understanding host-plasmid interactions. in the present study, effect of plasmid loss and their subsequent introduction on various properties of the host was evaluated. loss of plasmids caused a reduction in bacterial size and also slowed down their growth rate, μ, and respiratory rate, r. both μ and r values were partially recovered in c. renale r, obtained by retransformation of ...201021087624
composition and characteristics of urinary calculi from guinea pigs.to determine the mineral composition of calculi, anatomic locations of the calculi, and findings of urinalysis and bacteriologic culture of urine and calculi in guinea pigs with urolithiasis.200919210239
corynebacterium timonense sp. nov. and corynebacterium massiliense sp. nov., isolated from human blood and human articular hip fluid.gram-positive, facultatively anaerobic, rod-shaped bacteria were isolated from the blood of a patient with endocarditis (strain 5401744t) and from the hip joint fluid of a patient with an infected orthopaedic prosthesis (strain 5402485t). these strains were characterized by using a polyphasic taxonomic approach. based on cellular morphology and biochemical criteria the two isolates were tentatively assigned to the genus corynebacterium, although they did not correspond to any recognized species. ...200919567562
identification and characterization of antimicrobial peptide, defensin, in the taiga tick, ixodes persulcatus.ixodes persulcatus is the primary vector for human tick-borne diseases in japan. a cdna library was constructed from whole body homogenates of fed nymphs of i. persulcatus. from this library, one cdna encoding defensin-like antimicrobial peptide was identified. the amino-acid sequence showed high similarity to those of the defensins of other ticks and arthropods. i. persulcatus defensin mrna transcripts were detected at all life cycle stages of fed ticks and found to be predominantly expressed i ...200919604312
clinical and ultrasonographic findings, diagnosis and treatment of pyelonephritis in 17 cows.the goal of the present study was to describe the clinical, haematological and ultrasonographic findings and treatment of 17 cattle with pyelonephritis. fifteen cattle had an abnormal general condition, which varied in severity; five animals had signs of colic. the urine was brownish-red in 11 animals and cloudy in 13. clumps of purulent material were seen in the urine of nine animals and clots of blood in two. the specific gravity was lower than normal in 13 animals and ranged from 1.005 to 1.0 ...200817363303
corynebacterium pseudotuberculosis and corynebacterium renale isolated from two arabian oryx (oryx leucoryx). 200818587065
development of expression vectors for escherichia coli based on the pcr2 replicon.recent developments in metabolic engineering and the need for expanded compatibility required for co-expression studies, underscore the importance of developing new plasmid vectors with properties such as stability and compatibility.200717490494
corynebacterial necrohemorrhagic cystitis in two female macaques.we describe severe necrohemorrhagic cystitis in a female rhesus macaque and a female cynomolgus macaque due to colonization of the urinary bladder by corynebacterium sp. clinically, both macaques presented with perineal bleeding and depression and, despite extensive and prolonged treatment, succumbed to the disease. at necropsy, the contents of the urinary bladders in both cases were hemorrhagic to greenish black, and the bladder mucosa was necrotic. the major microscopic finding in each case wa ...200717994677
sonographic application in the diagnosis of pyelonephritis in cattle.nineteen cattle with pyelonephritis were examined sonographically. a 3.5- and 5.0-mhz convex transducer was used for percutaneous and a 5.5-mhz linear scanner for transrectal examination. ultrasonographic changes included enlarged kidneys, echogenic material, and flocculent fluid within a dilated and deformed renal sinus and a poor cortical medullary differentiation. azotemia was detected in 10 and hematuria and proteinuria in all animals. microbial culture revealed corynebacterium renale in eig ...200717236364
a herd level analysis of urinary tract infection in dairy cattle.cystitis, urethritis and pyelonephritis in cattle most commonly result from ascending urinary tract infection with corynebacterium renale, corynebacterium cystidis, corynebacterium pilosum or escherichia coli. we describe the clinical, bacteriological, clinical-pathological and epidemiological findings in a dairy cattle herd with urinary tract infection (uti). blood and urine samples from 17 calves and 19 cows were submitted to laboratory examinations. depression, muscle wasting, weakness and fr ...200616310382
characterization of broad host range cryptic plasmid pcr1 from corynebacterium renale.plasmid pcr1 is a cryptic plasmid harboured by corynebacterium renale. it is the smallest corynebacterial plasmid known to date. although its natural host is animal corynebacteria, it can replicate in several strains of soil corynebacteria. it can also replicate in escherichia coli, in which it is stably maintained. the copy number of pcr1 in this host is higher than that of puc19, with which it shows unidirectional incompatibility. it is also incompatible with pbk2, a plasmid bearing the common ...200616545871
high prevalence of ulcerative posthitis in rasa aragonesa rams associated with a legume-rich diet.this study describes an outbreak of ulcerative posthitis that eventually affected 76 of 80 of rams in a flock of rasa aragonesa sheep on a legume-rich diet. lesions were confined to the prepuce and varied from mild hyperaemia to ulcerations. corynebacterium renale was isolated from the lesions. treatment of an initial group of 17 was based on a change of diet and topical treatment with veterin banedif with prednisolone once a day for 15 days and was successful. lesions in this group were complet ...200515882402
slaughterhouse survey of pyelonephritis in dairy cows.twenty-one rejected kidneys from 2426 slaughtered dairy cows (0.87 per cent) had gross signs of pyelonephritis that were confirmed by histopathology. in all the kidneys the findings were consistent with a chronic rather than an acute infection. one species of bacteria was cultured from 12 of the kidneys and two species of bacteria were cultured from six. the most commonly isolated bacteria were escherichia coli, from eight kidneys, arcanobacterium pyogenes, from seven kidneys and corynebacterium ...200516299366
cystoscopy in cattle--a valuable additional tool for clinical examination.based on the findings of physical examination and on laboratory findings the urinary bladder of 23 cows was examined endoscopically in order to investigate the application of cystoscopy in cattle. the endoscopic findings of all examined cows were compared with the findings of physical examination and the results of macroscopic and microscopic urinalysis and the bacteriological culture of the urine. by physical examination only 3 cows were diagnosed to have urinary tract disease, whereas all othe ...200415595276
four-year survey of urinary tract infections in calves in israel.the prevalence of urinary tract infections in calves aged seven days to three months in three dairy cattle herds ranged from 0.5 to 1.6 per cent, with an average of 1.1 per cent. the mortality rate reached 16.1 per cent. the morbidity rate of the female calves was 1.4 per cent and that of the male calves 0.8 per cent. the bacteria isolated from urine, and from vaginal and preputial swabs were escherichia coli (35 per cent), corynebacterium renale (14 per cent), plasma coagulase-negative staphylo ...200414994858
biofilm bacteria: formation and comparative susceptibility to antibiotics.the calgary biofilm device (cbd) was used to form bacterial biofilms of selected veterinary gram-negative and gram-positive pathogenic bacteria from cattle, sheep, pigs, chicken, and turkeys. the minimum inhibitory concentration (mic) and minimum biofilm eradication concentration (mbec) of ampicillin, ceftiofur, cloxacillin, oxytetracycline, penicillin g, streptomycin, tetracycline, enrofloxacin, erythromycin, gentamicin, tilmicosin, and trimethoprim-sulfadoxine for gram-positive and -negative b ...200211989739
isolation of toxigenic nocardiopsis strains from indoor environments and description of two new nocardiopsis species, n. exhalans sp. nov. and n. umidischolae sp. nov.nocardiopsis strains were isolated from water-damaged indoor environments. two strains (n. alba subsp. alba 704a and a strain representing a novel species, es10.1) as well as strains of n. prasina, n. lucentensis, and n. tropica produced methanol-soluble toxins that paralyzed the motility of boar spermatozoa at <30 microg of crude extract (dry weight) x ml(-1). n. prasina, n. lucentensis, n. tropica, and strain es10.1 caused cessation of motility by dissipating the mitochondrial membrane potenti ...200111526036
corynebacterium renale infection in calves. 200010674699
toxigenic strains of bacillus licheniformis related to food poisoning.toxin-producing isolates of bacillus licheniformis were obtained from foods involved in food poisoning incidents, from raw milk, and from industrially produced baby food. the toxin detection method, based on the inhibition of boar spermatozoan motility, has been shown previously to be a sensitive assay for the emetic toxin of bacillus cereus, cereulide. cell extracts of the toxigenic b. licheniformis isolates inhibited sperm motility, damaged cell membrane integrity, depleted cellular atp, and s ...199910508100
[an experimental study of infection stone formation by corynebacterium species].the relationship between infection stone and corynebacterium species was investigated in vitro and in vivo. urease activity of urease-splitting corynebacterium species was evaluated by 2 methods; an increase in ph of human urine after inoculation of corynebacterium species and direct measurement of urease activity of 10(7) cfu organisms from amounts of ammonia by indophenol method. formation of infection bladder stone was induced in male wistar rats by implanting a zinc disc and inoculating 10(6 ...19979086346
purification and characterization of staphylococcin bacr1, a broad-spectrum bacteriocin.the bacteriocin bacr1 was purified from culture supernatant of staphylococcus aureus ut0007 by sequential ammonium sulfate precipitation, cation-exchange chromatography, and c4 reverse-phase chromatography steps. mass spectrographic analysis indicated that the purified peptide has a molecular mass of 3,338 da. it is resistant to environmental conditions, retaining full biological activity after exposure to ph extremes (phs 3 to 11), heating at 95 degrees c for 15 min, and exposure to strong chao ...19979361402
[nephrectomy for chronic, unilateral suppurative pyleonephritis in cattle].this report presents the case history of a five-year-old eringer cow suffering from chronic hematuria. results of clinical examination, ultrasonography of the kidneys, endoscopy of the bladder, and cytologic and bacteriologic analysis of the urine revealed unilateral pyelonephritis of the right kidney caused by corynebacterium renale. antimicrobial treatment with procaine penicillin was not successful. therefore, nephrectomy of the affected kidney was performed through a right flanc approach in ...19968720950
purification and partial characterization of a novel antibacterial agent (bac1829) produced by staphylococcus aureus ksi1829.a novel antimicrobial agent from staphylococcus aureus ksi1829, designated bac1829, was purified by sequential steps of ammonium sulfate precipitation, sephadex g-50 gel filtration chromatography, and hydrophobic interaction chromatography. purified bac1829 has a molecular mass of 6,418 +/- 2 da. the peptide in heat stable, since full biological activity is retained after heating at 95 degrees c for 15 min, and it is destroyed by digestion with proteases. amino acid sequence analysis revealed a ...19968795206
distribution of corynebacterium renale among apparently healthy rats.we examined the distribution of corynebacterium renale, a causative agent of urinary calculus, in clinically normal rats at 6 animal facilities in japan. swabs of the vulva and vaginal vestibule or prepuce of the rats were cultured for isolation of the organisms. c. renale has been isolated at only one animal facility, where cases of urinary calculus were reported several years ago. in this facility, 32% of female (43/135) and 22% of male (18/82) rats, 4-28 weeks old, were positive for c. renale ...19968972057
suspected venereal spread of corynebacterium renale. 19958533241
partial characterization of small plasmids from corynebacterium renale.a naphthalene-degrading strain of corynebacteria, corynebacterium renale, harbors multiple small plasmids designated pcr1, pcr2, pcr3, and pcr4 with sizes of 1.4, 3.2, 4.4, and 5.7 kb, respectively. plasmid pcr1 of 1.4 kb is the smallest plasmid reported in this group of bacteria and is present in high copy number. attempts to clone whole pcr1 in escherichia coli were unsuccessful but two of its fragments (750 and 650 bp) could be separately cloned in it. the 4.4-kb plasmid, pcr3, bears consider ...19958825376
assignment of the bacterial agent of urinary calculus in young rats by the comparative sequence analysis of the 16s rrna genes of corynebacteria.comparative 16s rrna gene sequencing was used to assign four isolates of spontaneous urinary calculus in young laboratory rats. the phylogenetic relationships among the rat isolates and selected species of corynebacteria were also inferred. based on the homology and evolutionary distance analysis, the 16s rrna genes of the rat isolates were almost identical with that of corynebacterium renale atcc 19412. also the results of the phylogenetic analysis showed a close relationship among the isolates ...19957548409
spontaneous urinary calculus in young lew rats caused by corynebacterium renale.spontaneous urinary calculus was observed in only 27% of female lew rats among nine inbred strains maintained in the institute for animal experimentation. the condition occurred between 3 and 9 weeks of age with loss of weight, enlargement of the os urethral externum, anuria and general marasmus. all of the affected rats died from anuria 3 to 4 days after the onset. the affected rats had one to ten infected stones or many fine crystals of sand, which consisted of magnesium ammonium phosphate, in ...19948051721
osteomyelitis and disseminated infection caused by corynebacterium renale in a goat.a 1.5-year-old female goat was examined for recurrence of lameness involving the right forelimb. radiography of the thorax and right scapulohumeral joint revealed a pathologic fracture of the supraglenoid tubercle, and circumscribed radiolucent lesions in the right third and fourth ribs, and the base of the spinous process of t3. bone scintigraphy demonstrated additional lesions in the lumbar spine and the wings of the ilium. at necropsy, disseminated infection and hematogenous osteomyelitis wer ...19948188516
the goat as a model for corynebacterium renale pyelonephritis.nubian goats were experimentally infected with corynebacterium renale type ii by either the intravenous or intraurethral routes using infection rates of 1.75 x 10(10), 7.08 x 10(19) or 5 x 10(23) organisms. all inoculated goats were anorexic, lost weight and became dull or depressed. albuminuria, pyuria and epithelial casts were noted in the urine. following intravenous challenge the animals showed a dose-related elevation of serum ammonia, urea, and creatinine with significant changes in haemog ...19938298960
numerical analysis of fatty and mycolic acid profiles of corynebacterium urealyticum and other related corynebacteria.the fatty and mycolic acid profiles of 52 strains of clinical origin belonging to corynebacterium urealyticum were subjected to numerical analysis along with those of representative members of corynebacterium ammoniagenes, corynebacterium bovis, corynebacterium glutamicum, corynebacterium jèikeium, corynebacterium minutissimum, corynebacterium pseudodiphtheriticum, corynebacterium pseudotuberculosis, corynebacterium xerosis, corynebacterium renale, corynebacterium cystitidis, "corynebacterium ul ...19938397966
[urinary tract infection caused by corynebacterium sp.--a case report and an experimental study].we repose of chronic cystitis associated with alkali urine, struvite stones and a subsequent vesicorectal fistula caused by corynebacterium sp, probably corynebacterium group d2. we also studied in vitro and in vivo stone formation caused by corynebacterium renale isolated clinically. c. renale inoculated into normal human urine increased urine ph and formed struvite crystals after a 24-hr incubation. bladder stones were also formed in rats experimentally infected by c. renale as well as p. mira ...19921529815
normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (macaca mulatta).the most common bacterial species isolated from the vaginas of 37 healthy rhesus macaques were streptococcus viridans, coagulase negative staphylococcus, mobiluncus curtisii ss. curtisii, corynebacterium renale-like organisms, peptostreptococcus anaerobius, gardnerella-like organisms, and other corynebacterium species. the vaginal flora of the rhesus macaque differs from that previously reported for five other primate species. a two-year retrospective review of clinical cases of vaginitis and me ...19911803013
isolation of corynebacterium renale from slaughtered cattle at the zaria abattoir in nigeria.forty urinary bladders were collected from apparently healthy cattle slaughtered at the zaria abattoir. twenty-four (60%) were from male animals and 16 (40%) from females. urine samples were obtained and cultured for corynebacterium renale. four (16.7%) of the samples from males and 1 (6.3%) from females were positive. the difference in infection between the sexes was statistically significant (p less than 0.001).19911818361
cloning and expression of a pili gene of corynebacterium renale in escherichia coli.a plasmid gene library of corynebacterium renale piliated strain no. 109p+ was prepared in escherichia coli in order to study the chemical structure of the pili of c. renale. of 3,000 recombinant clones tested, 5 reacted with anti-pili anti-serum. the gene products of these clones reacted with anti-pili monoclonal antibodies 8/4, 5/2 and b20/3 but lacked the reactivity with 13/4. sds-page analysis revealed that the expressed protein had a molecular mass of 48 kilodalton and deletion analysis sho ...19901968989
ph regulation of urease levels in streptococcus salivarius.potential mechanisms for regulation of urease levels in streptococcus salivarius were examined, including: induction by urea, nitrogen or carbon source repression, and effects of ph and co2 (because co2 enrichment enhanced urease detection on urea agar plates). regulation by either ph or co2 was confirmed by comparison of the urease accumulation pattern during anaerobic growth under co2 with that under n2. under co2, there was an initial buffering plateau at ph 6.2 and a rate of streptococcus sa ...19902110582
pyelonephritis in cows: 15 cases (1982-1986).medical records of 15 adult cows with a final diagnosis of pyelonephritis were evaluated retrospectively. only 3 cows had obvious clinical signs that indicated urinary tract disease. physical examination, including rectal and vaginal examinations, and urine chemical reagent strip screening detected abnormalities in all 15 cows and allowed subsequent confirmation by culture results of urine samples obtained using a catheter. bacteriologic cultures of urine revealed corynebacterium renale infectio ...19892649473
different species of corynebacteria in human investigations in cairo.in investigations undertaken in cairo of ocular smears the following corynebacteria were isolated: corynebacterium a4, corynebacterium a5, corynebacterium bovis, corynebacterium aquaticum, corynebacterium diphtheriae, corynebacterium equi, corynebacterium f2, corynebacterium kutscheri, corynebacterium minutissimi, corynebacterium renale, corynebacterium xerosis, corynebacterium group i, corynebacterium spec. (at present not classified). in this study the hitherto isolated species and possible hu ...19892689286
phagocytosis of piliated and non-piliated corynebacterium renale by murine polymorphonuclear leukocytes. 19882896261
population shift between piliated and non-piliated bacteria in various culture conditions of corynebacterium renale. 19882907765
comparison of experimental infection in mice of corynebacterium renale piliated and non-piliated clones. 19872887685
comparison of susceptibility to phagocytosis by mouse peritoneal macrophages between corynebacterium renale piliated and non-piliated clones. 19872887686
comparison of surface hydrophobicity of piliated and non-piliated clones of corynebacterium renale and corynebacterium pilosum.piliated (p+) and non-piliated (p-) clones of corynebacterium renale and c. pilosum were similar in hydrophobicity as measured by hydrophobic interaction chromatography, bacterial adherence to hydrocarbons and the salt aggregation test. therefore, the previously reported adherence of p+ clone to various cells, which is more effective than that of p- clone, may be uncorrelated with the degree of hydrophobicity of both clones of these bacteria. hydrophobicity of p+ and p- clones was found to be hi ...19872889288
population shift from piliated to non-piliated bacteria in kidneys, bladder and urine of mice infected with corynebacterium renale strain no. 115 piliated bacteria. 19872892960
isolation and characterization of monoclonal antibodies against pili of corynebacterium renale and corynebacterium pilosum.five monoclonal antibodies against pili of corynebacterium renale 115 p+ (piliated clone) and two monoclonal antibodies against pili of c. pilosum 92 p+ (piliated clone) were produced. these antibodies bound to pili of the homologous strain in in enzyme-linked immunosorbent assay (elisa) and agglutinated p+ but not p- (non-piliated clone) of each homologous strain. the five monoclonal antibodies against c. renale 115 p+ pili were divided into 2 groups, comprising 16/5, 160/1 and 32/6 and 13/4 an ...19872433832
causative agents of bovine pyelonephritis: corynebacterium renale, c. pilosum and c. cystitidis. 19863078648
production of trans-o-hydroxybenzalpyruvate from 1,2-dihydroxynaphthalene by cell-free extracts of corynebacterium renale. 19863770791
appearance of immunoglobulin classes and complement (c3) during corynebacterium renale-induced experimental pyelonephritis in the rat.the local appearance of various immunoglobulin (ig) isotypes in the urinary tract during ascending pyelonephritis was studied in rats experimentally infected with corynebacterium renale. the indirect fluorescent antibody assay was used to detect igg, igm, iga, ige, and c3 on c renale present in the urine of the experimental animals. corynebacterium renale coated with igm and igg antibodies was found beginning on the 4th day after induced infection, with igg being the more abundant isotype. coati ...19853888008
camp test for presumptive differentiation of corynebacterium renale and other bovine urinary corynebacteria. 19853892125
adhesion of corynebacterium renale and corynebacterium pilosum to epithelial cells of bovine vulva.corynebacterium renale and c pilosum adhered effectively to the epithelial cells of the bovine vulva; the numbers of these organisms that adhered to the vulval epithelial cells were 50 and 30/cell, respectively, which were several times as many as those that adhered to the uroepithelial cells. of the epithelial cells of the vulva, cornified cells lacking nuclei bound more bacteria than did those with indistinct nuclei, indicating that adhesion of bacteria was most effective to the most aged cell ...19853994106
adhesion of corynebacterium renale and corynebacterium pilosum to the epithelial cells of various parts of the bovine urinary tract from the renal pelvis to vulva.the various parts of the bovine urinary tract, the renal pelvis, the ureter, the urinary bladder, the urethra, the vaginal vestibule and the vulva, were examined for the capacity of the epithelial cells to bind corynebacterium renale and c. pilosum. c. renale adhered best to the epithelial cells of the vulva, and then to those of the ureter and renal pelvis. c. pilosum also adhered best to the epithelial cells of the vulva, followed by those of the vaginal vestibule. the results indicate that th ...19854002609
characterization of prosthetic groups of naphthalene oxygenase from corynebacterium renale. 19854030001
survival of corynebacterium renale, corynebacterium pilosum and corynebacterium cystitidis in soil.survival of the causative agents of bovine pyelonephritis, corynebacterium renale, c. pilosum and c. cystitidis, was examined at 30 degrees c in autoclaved soil. in the soil from a paddock, c. renale and c. cystitidis survived for 56 and 63 days, respectively, and c. pilosum for a longer period of at least 210 days. in soil from a pasture, sand from an athletic field and sea sand, the survival of these bacteria was of shorter duration.19854035958
further observations of corynebacterium renale as an indicator organism in the detection of the exfoliation-positive strains of staphylococcus aureus.exfoliatin-positive s. aureus strains of known phenotypes were examined for bacteriocin production using s. aureus 209p, c. pseudodiphtheriticum, or c. renale as indicator strains. strains of the phenotype bac+ tox+ had a positive result when the simultaneous method was used, and produced a wide inhibition of the indicator strains, especially of c. renale, if the deferred method was used. the strains of the phenotype bac- tox+ were negative upon examination by the simultaneous method, but they c ...19836369832
adhesion of corynebacterium renale, corynebacterium pilosum, and corynebacterium cystitidis to bovine urinary bladder epithelial cells of various ages and levels of differentiation.the adhesion of corynebacterium renale, corynebacterium pilosum, and corynebacterium cystitidis to various epithelial cell layers of bovine urinary bladders was examined. adhesion was most efficient to the urinary sediment epithelial cells and the superficial cells immediately before shedding, followed by the remaining superficial cells and intermediate cells in this order, and least efficient to the deeper intermediate and basal cells. incubation of the intermediate cells for 6 h increased the ...19827095847
mechanism of hemolysis by renalin, a camp-like protein from corynebacterium renale.synergistic hemolysis of sheep erythrocytes caused by staphylococcus aureus and corynebacterium renale resulted from the combined action of extracellular staphylococcal sphingomyelinase c and a newly described extracellular agent of c. renale (renalin). the affinity of renalin for ceramide was considered to play a key role in causing hemolysis in erythrocytes in which ceramide had been generated through the action of sphingomyelinase c.19827095849
scanning electron microscopy of experimentally induced pyelonephritis in the rat.scanning electron microscopy was used to observe epithelial and inflammatory changes in kidneys of rats during corynebacterium renale-induced experimental ascending pyelonephritis. bacteria were not observed adhering to pelvic epithelium, although there was evidence of cell sloughing. bacteria was observed in the interstitium of the renal medulla. bacteria, when present in the tubular lumen, were associated with discontinuities in cell membranes. phagocytic cells appeared in the interstitium and ...19817258790
evaluation of penicillin in conjunction with acetohydroxamic acid in treatment of experimental corynebacterium renale pyelonephritis.the therapeutic efficacies of procaine penicillin g and acetohydroxamic acid, when administered alone or in combination, were evaluated in rats with experimentally induced pyelonephritis at 24 h and 4 days after intrabladder inoculation with corynebacterium renale. penicillin alone prevented the development of pyelonephritis when administered in a once daily dose of 50,000 units for 5 days beginning within 24 h of infection challenge. however, if therapy was not initiated until the 4th day after ...19817332881
purification and characterisation of naphthalene oxygenase from corynebacterium renale.naphthalene oxygenase has been purified 420-fold from naphthalene-adapted corynebacterium renale. the purified enzyme was obtained in 32% yield and gave an apparent single band on polyacrylamide gel electrophoresis. it has a molecular weight of approximately 99,000 and contains two non-identical polypeptide chains of molecular weights 43,000 and 56,000. the enzyme requires molecular oxygen for its activity and gave a single products, cis-1,2-dihydroxy-1,2-dihydronaphthalene. the absorption spect ...19817333274
corynebacterium renale pyelonephritis and cystitis in a sheep. 19817340063
some experiments on ascending of corynebacterium renale in mice from urinary bladder into kidneys. 19817343690
an unusual site of corynebacterium renale infection in a deer. 19816916526
isolation of non-piliated clone of corynebacterium renale strain 115. 19816123610
ph-dependent adhesion of piliated corynebacterium renale to bovine bladder epithelial cells.the adhesion of corynebacterium renale 115 to bovine bladder epithelial cells was studied with two clones of the bacteria, piliated (p+) and nonpiliated (p-). the percentage of the cells with attached p+ bacteria was greater than that of the cells with attached p- bacteria, and the number of attached p+ bacteria was higher than that of the p- bacteria. the adhesion of p+ bacteria was found to be ph dependent, which was supported by the following facts: the number of attached bacteria was high at ...19806105129
specific immunofluorescence of corynebacterium renale. 1979367528
antibody coated bacteria in urine sediment from cattle infected with corynebacterium renale.using the fluorescent antibody test, the presence of antibody-coated bacteria in 10 out of 17 urine sediment samples from cattle infected with corynebacterium renale is described. these antibodies were mainly of the immunoglobulin class igg, and to a lesser extent iga. this finding is characteristic for infections of the upper urinary tract (pyelonephritis). in seven samples no antibody coating of the bacterial surface was detected. in these cases an infection of the lower urinary tract (cystiti ...1979390848
ulcerative posthitis in bulls in uruguay.ulcerative posthitis in bulls in uruguay is described; 1096 bulls in 17 different establishments were examined. the incidence of bulls grazing on improved pasture was 32.9% in those under one year and 80.8% in animals of 18 to 36 months of age. bulls grazing on natural pasture showed an incidence of 63.5% in adult animals irrespective of age. histologic characteristics of the preputial lesions were acanthosis, parakeratosis and hyperkeratosis followed by invasion of leukocytes in the epithelium ...1979455992
antibody formation in corynebacterium renale-induced experimental pyelonephritis in the rat.the immune defense system of the kidney was studied by inducing ascending pyelonephritis in rats with corynebacterium renale. with the fluorescent antibody technique, c renale organisms were observed in the renal pelvis, but were not coated with antibody until they reached the medulla. histopathologic evaluation of renal tissues collected serially after inoculation confirmed the presence of infection in the medulla when antibody coating occurred. serum anti-c renale antibody concentrations incre ...1979525896
[a trial on nutritionally forced mating of conventional glutamate-producing bacteria].by nutritionally forced mating, we tried to find the sex factor in glutamate producing bacteria with some auxtrophic mutants of corynebacterium glutamicum, micrococcus glutamicus and brevibacterium divaricatum. we failed to find true mates in this trial, even when the cells cured by acridine orange were used to mate with non-cured ones. there were some positive growth when two densed cell suspensions were dropped on the minimum medium, but after subcultured on the minimum medium no growth was fo ...1979583276
minimal nitrogen requirements of corynebacterium renal strains.corynebacterium renale strain 10849 was grown in a chemically defined medium containing glucosamine, ammonium sulfate, and 5 amino acids as possible nitrogen sources. although glucosamine was slightly stimulatory, its omission from the medium had a minimal effect on growth, and washed cells introduced into glucosamine-free medium grew readily through 10 serial transfers, demonstrating that this compound was not required for growth. individual omissions of isoleucine, valine, methionine, and glut ...1978629434
pili-mediated attachment of corynebacterium renale to mucous membrane of urinary bladder of mice.corynebacterium renal strain 115 with numerous pili became attached in vivo to the mucous membrane of the urinary bladder of mice 10 to 30 times more frequently than did that of c renale american type culture collection 19412, which showed few pili. antipili serum-treated c renale strain 115 was not recovered from the membrane in as large amounts as was untreated bacteria. antisomatic serum-treated strain 115, on the other hand, was recovered from the membrane in amounts similar to untreated bac ...1978629438
influence of iron on corynebacterium renale-induced pyelonephritis in a rat experimental model.growth of corynebacterium renale in vitro on low-iron medium (1.34 micron) was only slightly less than that on high-iron media (7.16 and 9.85 micron). however, studies on c. renale-induced pyelonephritis using the rat as an experimental model revealed that c. renale cultivated in high-iron media was capable of producing pyelonephritis, but when grown on low-iron medium, these bacteria were noninfective. this apparent avirulence of the bacteria cultivated on low levels of iron could be reversed b ...1978689735
experimental model of corynebacterium renale pyelonephritis produced in mice.corynebacterium renale type i (strain 115), 1.7 x 10(7) to 4.5 x 10(7) organisms, introduced intravenously into mice disappeared from the blood less than 24 h after inoculation and did not produce pyelonephritis. the same strain, 1 x 10(7) to 5 x 10(7) organisms, inoculated into the urinary bladder of mice was not recovered from the blood in any of the mice, but caused pyelonephritis accompanied by ureteritis and cystitis in 16 of 21 (76%) mice. pyelonephritis and cystitis in mice were histopath ...1977873610
experimental rat model for corynebacterium renale-induced pyelonephritis.the laboratory rat was able to serve as a model for ascending pyelonephritis after implantation of a zinc disk coated with corynebacterium renale into the urinary bladder because it satisfied three different criteria for infection. the production of an alkaline urine and the presence of significant numbers of c. renale in the kidneys, as well as distinct pyelonephritic lesions as revealed by histological examination, were observed in all rats infected with c. renale. control rats that harbored s ...1977591068
cell wall sugars and amino acids of three immunological types of corynebacterium renale. 1977599753
no effect of cyclophosphamide on ascending of corynebacterium renale from urinary bladder into kidneys in mice. 1977599754
influence of acetohydroxamic acid on experimental corynebacterium renale pyelonephritis.the role of corynebacterium renale urease in the establishment of pyelonephritis was studied by the oral administration of acetohydroxamic acid (aha), a urease inhibitor, to experimentally infected rats. the bacteria were introduced by surgical insertion of a zinc disc containing 1 x 10(6) colony-forming units of c-renale into the urinary bladder whereas sterile discs were implanted in the bladders of the control animals. daily administration of aha via the drinking water did not halt the develo ...197721738
effect of hydroxamic acids on growth and urease activity in corynebacterium renale.studies were conducted on the effect of four different hydroxamic acids (ha), hydroxyurea, acetohydroxamic acid, p-flurobenzoylhydroxamic acid and sorbylhydroxamic acid, on the growth and urease activity of corynebacterium renale. the addition of each of these ha, at concentrations ranging form 10(-3) to 10(-5) m, to medium containing urea as the sole nitrogen source resulted in a lengthened lag period of growth the extent of which depended upon the concentration of each ha tested as well as the ...19761260545
a numerical taxonomic study of the strains of three types of corynebacterium renale.numerical analysis has been carried out on 100 features of 55 strains of corynebacterium renale. three phena were formed, which respectively corresponded to three immunological types of c. renale.1975238729
distribution of corynebacterium renale among healthy bulls with special reference to inhabitation of type iii in the prepuce.distribution of corynebacterium renale among apparently healthy bulls reared in hokkaido was investigated. the organism was detected from 46 (39.3%) of 117 specimens of preputial cavity washing and from 60 (51.7%) of 116 specimens of semen. the isolates studied in this survey belonged to type iii, except a few which belonged to type ii. no type i strain was isolated from any bull. c. renale type iii was isolated from the prepuce in six of seven bulls slaughtered and from urethra in three, but no ...19751186911
attachment of corynebacterium renale to tissue culture cells by the pili.one or more cells of corynebacterium renale strains (serologic types, i, ii and iii), which possessed numerous pili, frequently were attached to bhk-21 cells, primary dog kidney cells, and primary rabbit kidney cells. the percentage of the cultured cells to which c renal cells were attached was about 70%. the percentage was less with cells of c renale possessing fewer pili, around 30%. after c renale was treated with the homologous anti-pili serum, the percentage of bhk-21 cells to which bacteri ...19751190607
some aspects of the immunological response in cattle infected with corynebacterium renale. identification of a new serotype of c. renale, type iv. 19751199528
efficacy of antibiotic treatment in cows affected with cystitis and those affected with pyelonephritis due to corynebacterium renale. 19751238865
[classification of corynebacterium renale]. 19751240301
[attachment of corynebacterium renale to animal cells by fimbria]. 19751240309
the diphtheroid agent of ovine posthitis: its relationship to corynebacterium renale. 19744142036
agglutination of trypsinized sheep erythrocytes by the pili of corynebacterium renale.corynebacterium renale strains which possessed numerous pili agglutinated trypsinized sheep erythrocytes, whereas those possessing only a few pili did not show such activity. by electron microscopy, the pili of c. renale adhered to the ghost of trypsinized sheep erythrocytes. after the treatment of c. renale with the homologous anti-pili serum, neither the agglutination of trypsinized sheep erythrocytes nor the adherence to the ghost by the pili were found. the adhesive property of the pili of c ...19744373397
[symposium i: cilia of corynebacterium renale]. 19744857936
comparison of the chemical and immunological properties of the pili of three types of corynebacterium renale. 19734633544
antibody response in cows infected with corynebacterium renale with special reference to the differentiation of pyelonephritis and cystitis. 19724552939
corynebacterium renale as a cause of reactions to the complement fixation test for johne's disease. 19724561547
chemical properties of the pili of corynebacterium renale.pili separated from the cells of corynebacterium renale strain 46 (type ii) by agitation at high speed in a homogenizer were purified by repeated cycles of ammonium sulfate precipitation, sonic treatment, and centrifugation. the preparation of purified pili formed a single antigen-antibody line in agar gel and showed an absorption maximum at 275 nm. the pili subjected to dodecyl sulfate-polyacrylamide gel electrophoresis formed a main band and corresponded to the molecular weight of 19,000. the ...19724632466
the rate of urea hydrolysis as a diagnostic criterion for corynebacterium renale. 19725072579
virulence of three types of corynebacterium renale in cows. 19715545463
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