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procalcitonin levels in bloodstream infections caused by different sources and species of bacteria.the aim of this study was to evaluate procalcitonin (pct) diagnostic accuracy in discriminating gram-negative (gn) from gram-positive (gp) bloodstream infections and determining the relationship between pct levels, infection sites, and pathogen types.201727979420
[antimicrobial resistance trends in pathogens isolated from nosocomial infections].the treatment of nosocomial infections is hindered by the increasing antimicrobial resistance pattern of germs that cause them. the objective was to assess trends in resistance of bacteria isolated from nosocomial infections.201726820196
behavioral response of the malaria mosquito, anopheles gambiae, to human sweat inoculated with axilla bacteria and to volatiles composing human axillary odor.the responses of anopheles gambiae giles sensu stricto (diptera: culicidae) to odors from male and female axillary sweat incubated with human axilla bacteria were recorded in a dual-choice olfactometer. staphylococcus epidermidis was selected for its low odor-producing pattern, corynebacterium jeikeium for its strong nα-acylglutamine aminoacylase activity liberating carboxylic acids including (r)/(s)-3-hydroxy-3-methylhexanoic acid (hmha) and staphylococcus haemolyticus for its capacity to liber ...201727789516
prevalence and genetic mechanisms of antimicrobial resistance in staphylococcus species: a multicentre report of the indian council of medical research antimicrobial resistance surveillance network.routine surveillance of antimicrobial resistance (amr) is an essential component of measures aimed to tackle the growing threat of resistant microbes in public health. this study presents a 1-year multicentre report on amr in staphylococcus species as part of indian council of medical research-amr surveillance network.201728303819
[investigation of biofilm formation properties of staphylococcus isolates].biofilm production is an important virulence factor which allows staphylococci to adhere to medical devices. the principal component of biofilm is a "polysaccharide intercellular adhesin (pia)" which is composed of a beta-1,6-n-acetylglucosamine polymer synthesized by an enzyme (n-acetylglucosamine transferase) encoded by the ica operon found on the bacterial chromosome. this operon is composed of four genes (a, b, c, and d), and a transposable element is256. in this study, we aimed to determine ...201728283006
exploring the binding sites of staphylococcus aureus phenylalanine trna synthetase: a homology model approach.increased resistance of mrsa (multidrug resistance staphylococcus aureus) to anti-infective drugs is a threat to global health necessitating the development of anti-infectives with novel mechanisms of action. phenylalanine trna synthetase (phers) is a unique enzyme of the aminoacyl-trna synthetases (aarss), which are essential enzymes for protein biosynthesis. phers is an (αb)2 tetrameric enzyme composed of two alpha subunits (phes) and two larger beta subunits (phet). our potential target in th ...201728235746
c-jun n-terminal kinase (jnk) is involved in immune defense against bacterial infection in crassostrea hongkongensis.c-jun n-terminal kinase (jnk) is a universal and essential subgroup of the mitogen-activated protein kinase (mapk) superfamily, which is highly conserved from yeast to mammals and functions in a variety of physiological and pathological processes. in this study, we report the first oyster jnk gene homolog (chjnk) and its biological functions in the hong kong oyster crassostrea hongkongensis. the chjnk protein consists of 383 amino acids and contains a conserved serine/threonine protein kinase (s ...201727840294
coagulase-negative staphylococcus species in bulk milk: prevalence, distribution, and associated subgroup- and species-specific risk factors.coagulase-negative staphylococci (cns) have become the main pathogens causing bovine mastitis in recent years. a huge variation in species distribution among herds has been observed in several studies, emphasizing the need to identify subgroup- and species-specific herd-level factors to improve our understanding of the differences in ecological and epidemiological nature between species. the use of bulk milk samples enables the inclusion of a large(r) number of herds needed to identify herd-leve ...201727865514
detection and genetic environment of pleuromutilin-lincosamide-streptogramin a resistance genes in staphylococci isolated from pets.increasing emergence of staphylococci resistant to pleuromutilins, lincosamides, and streptogramin a (plsa) and isolated from humans and pets is a growing public health concern worldwide. currently, there was only one published study regarding one of the plsa genes, vga(a) detected in staphylococci isolated from cat. in this study, eleven pleuromutilin-resistant staphylococci from pets and two from their owners were isolated and further characterized for their antimicrobial susceptibilities, pla ...201728261187
the lytic activity of recombinant phage lysin lyskδamidase against staphylococcal strains associated with bovine and human infections in the jiangsu province of china.we investigated the lytic activity of the bacteriophage endolysin (lysin) lyskδamidase against live methicillin-resistant and-susceptible staphylococcal strains clinically isolated from bovine milk and humans from different origins of china. antibiotic resistance patterns, multilocus sequence typing and sccmec type of 137 staphylococcal strains isolated from bovine milk associated with bovine mastitis and human diseases were studied. a lytic enzyme, lyskδamidase, was constructed by fusing the n- ...201728235706
molecular epidemiology of coagulase-negative bloodstream isolates: detection of staphylococcus epidermidis st2, st7 and linezolid-resistant st23.the mechanisms contributing to persistence of coagulase-negative staphylococci are diverse; to better understanding of their dynamics, the characterization of nosocomial isolates is needed. our aim was to characterize phenotypic and molecular characteristics of staphylococcus epidermidis and staphylococcus haemolyticus human blood isolates from two tertiary care hospitals in mexico, the hospital universitario in monterrey and the hospital civil in guadalajara. antimicrobial susceptibility was de ...201727393769
coagulase-negative staphylococci in southern brazil: looking toward its high diversity.coagulase-negative staphylococci (cons) are the most prevalent pathogens in nosocomial infections and may serve as a reservoir of mobile genetic elements such as the staphylococcal cassette chromosome mec (sccmec) encoding methicillin resistance. molecular characterization of sccmec types combined with advanced molecular typing techniques may provide essential information for understanding the evolution and epidemiology of cons infections. we therefore aimed to investigate the sccmec distributio ...201727384825
draft genome sequence of a canine isolate of methicillin-resistant staphylococcus haemolyticus.staphylococcus haemolyticus strain sw007 was isolated from a nasal swab taken from a healthy dog. the isolate is resistant to methicillin, mupirocin, macrolides, and sulfonamides. the sw007 draft genome is 2,325,410 bp and contains 2,277 coding sequences, including 60 trnas and nine complete rrna-coding regions.201728385855
methicillin susceptible teicoplanin resistant staphylococcus haemolyticus from bloodstream infection: novel mutation in teicoplanin resistant operon (tcarab).staphylococcus haemolyticus is the frequently isolated coagulase-negative staphylococci (cons) from blood culture. here we port a case of methicillin-susceptible s. haemolyticus resistant to teicoplanin and heteroresistant to vancomycin. the isolate was found to susceptible to cefoxitin and resistant to teicoplanin in e-test. pap-auc analysis confirmed the presence of vancomycin heteroresistant subpopulation. ngs analysis of the genome revealed the presence of blaz, msr(a) codes for cross-resist ...201728250264
sphygmomanometers and thermometers as potential fomites of staphylococcus haemolyticus: biofilm formation in the presence of antibiotics.the association between staphylococcus haemolyticus and severe nosocomial infections is increasing. however, the extent to which fomites contribute to the dissemination of this pathogen through patients and hospital wards remains unknown.201728225903
sphygmomanometers and thermometers as potential fomites of staphylococcus haemolyticus: biofilm formation in the presence of antibiotics.the association between staphylococcus haemolyticus and severe nosocomial infections is increasing. however, the extent to which fomites contribute to the dissemination of this pathogen through patients and hospital wards remains unknown.201728225897
molecular identification and functional characterization of a tumor necrosis factor (tnf) gene in crassostrea hongkongensis.tumor necrosis factor superfamily (tnfsf) represents a group of multifunctional inflammatory cytokines that have been shown to participate in a variety of pathological and immunological process. however, the functions of these proteins in oyster are still poorly understood. in the present study, an oyster tnf homolog (named chtnf) was identified from a cdna library of crassostrea hongkongensis. the complete cdna of chtnf was 2457bp in length containing an open reading frame (orf) of 1044bp, a 5' ...201728189340
occurrence of linezolid-resistant staphylococcus haemolyticus in two tertiary care hospitals in mysuru, south india. 201728179127
a molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro.extracellular signal-regulated kinases (erks) are a group of highly conserved serine/threonine-specific protein kinases that function as important signaling intermediates in mitogen-activated protein kinase (mapk) pathways, which are involved in a wide variety of cellular activities, including proliferation, inflammation and cytokine production. however, little is known about the roles of this kinase in mollusk immunity. in this study, we identified a molluscan erk homolog (cherk) in the hong ko ...201728159693
biofilm formation and antimicrobial resistance genes of coagulase-negative staphylococci isolated from cows with mastitis in argentina.mastitis affects the health and welfare of dairy cows worldwide. coagulase-negative staphylococci (cns) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. a total of 90 cns isolated from cows with clinical and subclinical mastitis in argentina from 2008-2014 were identified by pcr-rflp using the gap gene. standard microtiter plate assays were used to assess cns biofilm formation and staphylococcus haemolyticus species formed the st ...201728087612
coagulase-negative staphylococci causing blood stream infection at an indian tertiary care hospital: prevalence, antimicrobial resistance and molecular characterisation.recent years have seen a rise of coagulase-negative staphylococci (cons) from common contaminants to agents of nosocomial blood stream infections (bsi's). molecular typing and establishing a correlation with antibiotic resistance is essential particularly in countries like india where genotyping studies for drug-resistant cons are sparse.201727934830
postoperative endophthalmitis caused by staphylococcus haemolyticus following femtosecond cataract surgery.a 53-year-old caucasian man underwent femtosecond cataract surgery and then presented with pain and hand motions vision 1 day following surgery. anterior segment examination showed a 2-mm-layered hypopyon, a well-centered intraocular lens in the sulcus, and an obscured view to the fundus. b-scan ultrasonography showed significant vitritis and that the retina was attached. a tap and an injection of vancomycin 1 mg per 0.1 ml and of ceftazidime 2.25 mg per 0.1 ml were performed. the tap eventually ...201726951642
[antimicrobial sensitivity of the environmental microbiota in the intensive care units of a peruvian hospital].the objective was to detect gram-negative bacilli and gram-positive cocci isolated from the environmental microbiota of the intensive care unit (icu) departments of neonatology, pediatrics, and transplants (kidney, liver, and general) in a lima hospital and determine their antimicrobial sensitivity. eighty samples were obtained from inanimate surfaces using a wet swab. a total of 61 bacterial strains were identified, including staphylococcus epidermis (46.0%), alcaligenes sp. (21.3%), pseudomona ...201728538851
staged reimplantation of a total hip prosthesis after co-infection with candida tropicalis and staphylococcus haemolyticus: a case report.fungal prosthetic joint infection is a rare complication in total joint arthroplasty. there are no established guidelines for management of these infections. we present a case of a 53-year-old male with a hip joint prosthesis co-infected with candida tropicalis and staphylococcus haemolyticus. a two-stage exchange arthroplasty was performed. the patient underwent implant removal, debridement, irrigation with saline solution and application of cement spacer impregnated with vancomycin followed by ...201728735470
collaborative research on puerperal infections in bangladesh.bangladesh is considered as a high-risk country for emerging infectious diseases because of its high population density, poverty, and unhygienic conditions. although control efforts have primarily been focused on major infectious diseases such as diarrheal diseases, tuberculosis, malaria, and hiv infection, the prevalence and impact of many local or minor infectious diseases are still unclarified in this country. in this review, we present our recent experience and outcomes of collaborative rese ...201728552890
[effects of application of vancomycin in the early stage of patients with extremely severe burn].objective: to evaluate the effects of application of vancomycin in the early stage of patients with extremely severe burn, in order to provide reference to drug for anti-infection treatment in the early stage of patients with extremely severe burn. methods: data of 15 patients of kunshan explosion on august 2nd, 2014, admitted to the department of intensive care in our hospital were retrospectively analyzed. the clinical efficacy of continuously intravenous dripping of vancomycin (combined with ...201728427133
distribution of non-aureus staphylococci species in udder quarters with low and high somatic cell count, and clinical mastitis.the effect of non-aureus staphylococci (nas) in bovine mammary health is controversial. overall, nas intramammary infections (imi) increase somatic cell count (scc), with an effect categorized as mild, mostly causing subclinical or mild to moderate clinical mastitis. however, based on recent studies, specific nas may affect the udder more severely. some of these apparent discrepancies could be attributed to the large number of species that compose the nas group. the objectives of this study were ...201728456402
prevalence of non-aureus staphylococci species causing intramammary infections in canadian dairy herds.non-aureus staphylococci (nas), the microorganisms most frequently isolated from bovine milk worldwide, are a heterogeneous group of numerous species. to establish their importance as a group, the distribution of individual species needs to be determined. in the present study, nas intramammary infection (imi) was defined as a milk sample containing ≥1,000 cfu/ml in pure or mixed culture that was obtained from a cohort of cows assembled by the canadian bovine mastitis research network. overall, 6 ...201728527793
high prevalence of methicillin-resistant coagulase-negative staphylococci isolated from a university environment in thailand.the present study was conducted to isolate and characterize the molecular epidemiology of the methicillin-resistant staphylococci in the general university environment, where all five locations; the library, restrooms, canteens, computer rooms and outdoor surfaces, are in common use by a large population of students. we used mannitol salt agar (msa) supplemented with 4 μg/ml of oxacillin to screen the methicillin-resistant staphylococci. the species level was identified by pcr of rdr (staphyloco ...201728617524
phenol-soluble modulin toxins of staphylococcus haemolyticus.coagulase-negative staphylococci (cons) are important nosocomial pathogens and the leading cause of sepsis. the second most frequently implicated species, after staphylococcus epidermidis, is staphylococcus haemolyticus. however, we have a significant lack of knowledge about what causes virulence of s. haemolyticus, as virulence factors of this pathogen have remained virtually unexplored. in contrast to the aggressive pathogen staphylococcus aureus, toxin production has traditionally not been as ...201728596942
phenotypic characterisation of coagulase-negative staphylococci isolated from blood cultures in newborn infants, with a special focus on staphylococcus capitis.this swedish study determined which species of coagulase-negative staphylococci (cons) were found in neonatal blood cultures and whether they included staphylococcus capitis clones with decreased susceptibility to vancomycin.201728631328
quarter- and cow-level risk factors for intramammary infection with coagulase-negative staphylococci species in swiss dairy cows.bacteriological status, evaluation of udder symmetry, udder hygiene, and teat end scores of 92 dairy cows were assessed on 3 swiss dairy farms in a longitudinal 1-yr study to determine risk factors for intramammary infection (imi) with coagulase-negative staphylococci (cns) species. farm visits were performed monthly including sterile quarter milk sampling and udder evaluation of all lactating cows. milk samples were evaluated for the presence of staphylococci using selective agar plates. specie ...201728477997
biofilm formation of methicillin-resistant coagulase negative staphylococci (mr-cons) isolated from community and hospital environments.methicillin-resistant coagulase negative staphylococci (mr-cons) are the major cause of infectious diseases because of their potential ability to form biofilm and colonize the community or hospital environments. this study was designed to investigate the biofilm producing ability, and the presence of meca, icaad, bap and fnba genes in mr-cons isolates. the mr-cons used in this study were isolated from various samples of community environment and five wards of hospital environments, using mannito ...201728859149
changing epidemiology of coagulase-negative staphylococci in normal flora of skin.coagulase negative staphylococci (cons) have recently emerged as important pathogens causing nosocomial blood stream infections. to evaluate the prevalence of cons in cutaneous normal flora, skin swabs were collected from voluntary blood donors and processed for culture and identification using matrix assisted laser desorption ionisation-time of flight (maldi-tof). cons were isolated from 96% of blood donors, most commonly staphylococcus hominis (86%), followed by staphylococcus epidermidis (22% ...201728681820
coagulase-negative staphylococci in human milk from mothers of preterm compared with term neonates.human milk is the preferred nutrition for neonates and a source of bacteria. research aim: the authors aimed to characterize the molecular epidemiology and genetic content of staphylococci in the human milk of mothers of preterm and term neonates.201728418807
high-quality genome sequence of the highly resistant bacterium staphylococcus haemolyticus, isolated from a neonatal bloodstream infection.using illumina hiseq and pacbio technologies, we sequenced the genome of the multidrug-resistant bacterium staphylococcus haemolyticus, originating from a bloodstream infection in a neonate. the sequence data can be used as an accurate reference sequence.201728729278
prevalence of methicillin resistance and macrolide-lincosamide-streptogramin b resistance in staphylococcus haemolyticus among clinical strains at a tertiary-care hospital in thailand.staphylococcus spp. is a major cause of nosocomial infection and sepsis. however, increasing drug resistance is becoming a challenge to microbiologists. the purpose of this study was to identify and determine antimicrobial resistance phenotypes and drug resistance genes of clinical coagulase-negative staphylococci (cons) isolates at mae sot hospital in tak province, thailand. a total of 229 cons isolates were collected from clinical specimens during two periods in 2014 and in 2015. staphylococcu ...201728702199
investigation of biofilm formation in coagulase-negative staphylococci isolated from platelet concentrate bags.platelet concentrates (pcs) are the blood components with the highest rate of bacterial contamination, and coagulase-negative staphylococci (cons) are the most frequently isolated contaminants. this study investigated the biofilm formation of 16 contaminated units out of 691 pcs tested by phenotypic and genotypic methods. adhesion in borosilicate tube (abt) and congo red agar (cra) tests were used to assess the presence of biofilm. the presence of icaadc genes was assessed by means of the polyme ...201626910444
successful non-surgical treatment of endocarditis caused by staphylococcus haemolyticus following transcatheter aortic valve implantation (tavi).we describe a case of a 79-year-old male patient with severe aortic stenosis who underwent transcatheter aortic valve implantation (tavi) at our institution. he presented at the emergency department with dyspnea and fatigue 7 months after implantation. the diagnosis of early prosthetic valve endocarditis (pve) caused by staphylococcus haemolyticus was made by transesophageal echocardiography (tee) and multiple positive blood cultures. since our patient was considered inoperable due to a history ...201624579245
analysis of nosocomial staphylococcus haemolyticus by mlst and maldi-tof mass spectrometry.coagulase-negative staphylococci (cons) are a major component of normal human skin and mucosae flora. however, some species of cons can lead to infections in immunocompromised patients and premature newborns. the choice of a rapid and reliable typing method is one of the major problems in the epidemiological monitoring of cons, especially staphylococcushaemolyticus isolates. in this study, we have tested 71 isolates of s. haemolyticus obtained from newborns using the multilocus sequence typing ( ...201626723920
variable number of tandem repeat profiles and antimicrobial resistance patterns of staphylococcus haemolyticus strains isolated from blood cultures in children.staphylococcus haemolyticus is a healthcare-associated pathogen and can cause a variety of lifethreatening infections. additionally, multi-drug resistance (mdr), in particular methicillin-resistant s. haemolyticus (mrsh) isolates, have emerged. dissemination of such strains can be of great concern in the hospital environment. a total number of 20s. haemolyticus isolates from blood cultures obtained from children were included in this study. a high prevalence of mdr-mrsh isolates with high mic va ...201626656742
identification of chromium resistant bacteria from dry fly ash sample of mejia mtps thermal power plant, west bengal, india.eight chromium resistant bacteria were isolated from a dry fly ash sample of dvc-mtps thermal power plant located in bankura, west bengal, india. these isolates displayed different degrees of chromate reduction under aerobic conditions. according to 16s rdna gene analysis, five of them were staphylococcus, two were bacillus and one was micrococcus. the minimum inhibitory concentration towards chromium and the ability to reduce hexavalent chromium to trivalent chromium was highest in staphylococc ...201626602566
identification of novel sequence types among staphylococcus haemolyticus isolated from variety of infections in india.the aim of this study was to determine sequence types of 34 s. haemolyticus strains isolated from a variety of infections between 2013 and 2016 in india by mlst. the mega5.2 software was used to align and compare the nucleotide sequences. the advanced cluster analysis was performed to define the clonal complexes. mlst analysis showed 24 new sequence types (st) among s. haemolyticus isolates, irrespective of sources and place of isolation. the finding of this study allowed to set up an mlst datab ...201627824930
a novel p38 mapk indentified from crassostrea hongkongensis and its involvement in host response to immune challenges.p38 mitogen-activated protein kinases (mapks) are conserved serine/threonine-specific kinases that are activated by various extracellular stimuli and play crucial regulatory roles in immunity, development and homeostasis. however, the function of p38s in mollusks, the second most diverse group of animals, is still poorly understood. in this study, a novel molluscan p38 (designated chp38) was cloned and characterized from the hong kong oyster crassostrea hongkongensis. its full-length cdna encode ...201627768933
multidrug-resistant staphylococcus haemolyticus isolates from infected eyes and healthy conjunctivae in india.this study aimed to determine the presence of antibiotic resistance genes (args), sccmec elements and genetic relatedness among staphylococcus haemolyticus isolated from patients with a variety of eye infections (n=11) and from healthy conjunctiva (n=7). minimum inhibitory concentrations were determined for 14 antimicrobials according to bsac guidelines. pcr was used to identify the presence of meca, mecc, sccmec type and args. sequencing was used to determine mutations in gyra, gyrb, topoisomer ...201627530859
staphylococcus haemolyticus as a potential producer of biosurfactants with antimicrobial, anti-adhesive and synergistic properties.staphylococcus haemolyticus is an opportunistic human pathogen that usually gains entry into the host tissue in association with medical device contamination. biofilm formation is a key factor for the establishment of this bacterium and its arrangement and dynamics can be influenced by the synthesis of biosurfactants. biosurfactants are structurally diverse amphiphilic molecules with versatile biotechnological applications, but information on their production by staphylococci is still scarce. in ...201627377762
complete genome sequence of biofilm-forming strain staphylococcus haemolyticus s167.staphylococcus haemolyticus s167 has the ability to produce biofilms in large quantities. genomic analyses revealed information on the biofilm-related genes of s. haemolyticus s167. detailed studies of biofilm formation at the molecular level could provide a foundation for biofilm control research.201627313306
linezolid-resistant mucoid staphylococcus haemolyticus from a tertiary-care centre in delhi.we report an unusual morphological mucoid variant of staphylococcus haemolyticus associated with linezolid resistance from a patient with sepsis. linezolid resistance and mucoid character together made this pathogen difficult to treat. to our knowledge this is the first such report.201627274851
impact of insertion sequences and recombination on the population structure of staphylococcus haemolyticus.staphylococcus haemolyticus is one of the most common pathogens associated with medical-device related infections, but its molecular epidemiology is poorly explored. in the current study, we aimed to better understand the genetic mechanisms contributing to s. haemolyticus diversity in the hospital environment and their impact on the population structure and clinical relevant phenotypic traits. the analysis of a representative s. haemolyticus collection by multilocus sequence typing (mlst) has id ...201627249649
identification of odontella aurita by rbcl gene sequence - a high antibacterial potential centric marine diatom.the antibacterial potential of centric marine diatoms has been compared against the clinical pathogens and identified the potential diatom by rbcl gene sequencing. totally, five diatoms namely odontella aurita, thalassiosira subtilis, chaetoceros curvisetus, skeletonema costatum and coscinodiscus centralis were isolated from cuddalore coastal waters. the diatoms were morphologically identified and isolated using micro capillary-pipette and serial dilution method. the isolated diatoms were cultur ...201627159722
high prevalence of staphylococcus haemolyticus and staphylococcus saprophyticus in environmental samples of a tunisian hospital.the purpose of this study was to evaluate the rate of detection of coagulase negative staphylococci (cons) in environmental samples of 17 services in a tunisian hospital, determining the antimicrobial resistance phenotypes and genotypes of recovered isolates. to our knowledge, this is the first study that determines the prevalence of cons with correlation of antibiotic resistance in the hospital environment in tunisia. cons were obtained from 83 of the 200 tested samples (41.5%). staphylococcus ...201627133307
diversity of staphylococcal cassette chromosome mec elements in nosocomial multiresistant staphylococcus haemolyticus isolates.staphylococcus haemolyticus is the second, most frequently isolated coagulase-negative staphyloccus (cons) from patients with hospital-acquired infections, and it is usually resistant to methicillin and other semisynthetic penicillins. the purpose of this study was to characterize staphylococcal cassette chromosome mec (sccmec) elements and assess the in-vitro activity of antibiotics against 60 s. haemolyticus strains recovered from hospitalized patients. all these strains expressed methicillin ...201627056386
whole-genome sequences of staphylococcus haemolyticus isolated from infected eyes and healthy conjunctiva in bhubaneswar, india.staphylococcus haemolyticus, an opportunistic pathogen, is known to exhibit multidrug resistance and produce biofilm. we sequenced the genome of four multidrug resistant, biofilm forming isolates from infected eyes and asymptomatic healthy conjunctiva.201626988040
staphylococcus epidermidis and staphylococcus haemolyticus: detection of biofilm genes and biofilm formation in blood culture isolates from patients in a brazilian teaching hospital.infections with coagulase-negative staphylococci are often related to biofilm formation. this study aimed to detect biofilm formation and biofilm-associated genes in blood culture isolates of staphylococcus epidermidis and s. haemolyticus. half (50.6%) of the 85 s. epidermidis isolates carried the icaad genes and 15.3% the bhp gene, while these numbers were 42.9% and 0 for s. haemolyticus, respectively. according to the plate test, 30 s. epidermidis isolates were biofilm producers and 40% of the ...201627344542
short communication: prevalence, antimicrobial resistance, and resistant traits of coagulase-negative staphylococci isolated from cheese samples in turkey.a total of 17 coagulase-negative staphylococci (cns) isolates obtained from 72 cheese samples were included in this study. coagulase-negative staphylococci isolates obtained in this study comprised 6 (35.3%) staphylococcus saprophyticus, 3 (17.6%) staphylococcus epidermidis, 2 (11.8%) staphylococcus hominis, 2 (11.8%) staphylococcus haemolyticus, 1 (5.9%) staphylococcus xylosus, 1 (5.9%) staphylococcus vitulinus, 1 (5.9%) staphylococcus lentus, and 1 (5.9%) staphylococcus warneri. the disc diffu ...201626874419
antimicrobial, antibiofilm, and microbial barrier properties of poly (ε-caprolactone)/cloisite 30b thin films.development of antibacterial and antibiofilm surfaces is in high demand. in this study, nanocomposite of poly (ε-caprolactone)/cloisite 30b was prepared by the solvent casting method. the membranes were characterised by sem, afm, and ftir. evaluation of water uptake, antimicrobial, antibiofilm, and microbial barrier properties demonstrated a significant antimicrobial and antibiofilm activity against mtcc strain of staphylococcus haemolyticus and strong biofilm positive staphylococcus epidermidis ...201628330321
susceptibility profile of staphylococcus epidermidis and staphylococcus haemolyticus isolated from blood cultures to vancomycin and novel antimicrobial drugs over a period of 12 years.the aim of this study was to evaluate the antimicrobial susceptibility profile of 85 staphylococcus epidermidis and 84 staphylococcus haemolyticus strains isolated from blood cultures to oxacillin, vancomycin, tigecycline, linezolid, daptomycin, and quinupristin/dalfopristin over a period of 12 years. s. epidermidis and s. haemolyticus isolated from blood cultures of inpatients, attended at a teaching hospital, were analyzed for the presence of the meca gene and by sccmec typing. the minimum inh ...201626623676
phenotypical and genotypical antimicrobial resistance of coagulase-negative staphylococci isolated from cow mastitis.the objectives of this study were to determine the prevalence and antimicrobial resistance of coagulase-negative staphylococci (cns) isolated from dairy cows with subclinical mastitis. antimicrobial resistance in staphylococci were evaluated by breakpoint values specific to the species (eu-cast). the presence of resistance-encoding genes was detected by multiplex pcr. a total of 191 cns isolates were obtained. the cns isolates were typically resistant to penicillin (67.4%), tetracyc-line (18.9%) ...201627760017
prevalence and antibiotic susceptibility of coagulase-negative staphylococcus species from bovine subclinical mastitis in dairy herds in the central region of argentina.coagulase-negative staphylococci (cns) are a common cause of bovine subclinical mastitis (scm). the prevalence of cns species causing scm identified by genotyping varies among countries. overall, the antimicrobial resistance in this group of organisms is increasing worldwide; however, little information exists about a cns species resistant to antibiotics. the aim of the present study was to genotypically characterize cns at species level and to determine the prevalence and antibiotic resistance ...201626935912
teat apex colonization with coagulase-negative staphylococcus species before parturition: distribution and species-specific risk factors.coagulase-negative staphylococci (cns) are the main cause of bovine intramammary infections and are also abundantly present in extramammary habitats such as teat apices. teat apex colonization (tac) with cns has already been explored in lactating dairy cows at the species level, whereas this is not true for dry cows and end-term heifers. therefore, the aim of this observational study was to describe cns tac in nonlactating dairy cows and end-term heifers in flemish dairy herds and to identify as ...201626709171
microbiological contamination of drugs during their administration for anesthesia in the operating room.the aseptic techniques of anesthesiologists in the preparation and administration of injected medications have not been extensively investigated, but emerging data demonstrate that inadvertent lapses in aseptic technique may be an important contributor to surgical site and other postoperative infections.201626845141
nasal carriage of uncommon coagulase-negative staphylococci in nurses and physicians of tehran university hospitals.coagulase-negative staphylococci (cons) have been identified as a major cause of nosocomial infections. nasal carriage of cons in nurses and physicians is known to be an important risk factor for potential hospital infections. this study was carried out to investigate the prevalence of nasal carriage of uncommon coagulase-negative staphylococci among nurse and physician staffs of tehran university hospitals. a total of 116 cons were isolated from anterior nares of the study participants working ...201627309482
genotypic diversity of methicillin-resistant coagulase-negative staphylococci isolated from inpatients and outpatients.we investigated the prevalence of methicillin-resistant coagulase-negative staphylococci (mrcons) isolated from hospitalized patients and outpatients (op). out of 350 staphylococcal isolates collected from three hospitals, 190 were coagulase-negative staphylococci (cons). these isolates were subjected to antimicrobial susceptibility tests, detection of meca, and pulsed-field gel electrophoresis (pfge) typing. among the 190 isolated cons, staphylococcus epidermidis (47.3%) and staphylococcus haem ...201626248114
comparison of staphylococcal flora in denture plaque and the surface of the pharyngeal mucous membrane in kidney transplant recipients.biofilm occurring on removable prostheses is a reservoir of bacterial flora, consisting of both physiological and pathogenic multidrug-resistant microorganisms. patients undergoing dialysis and organ transplantation are particularly prone to bacterial infections, which can have its source in denture plaque. this study was a comparison of the composition of staphylococcal flora within the surface of the pharyngeal mucous membrane as well as denture plaque in kidney transplant recipients.201627496452
dissemination of novel antimicrobial resistance mechanisms through the insertion sequence mediated spread of metabolic genes.the widely used biocide triclosan selectively targets fabi, the nadh-dependent trans-2-enoyl-acyl carrier protein (acp) reductase, which is also an important target for the development of narrow spectrum antibiotics. the analysis of triclosan resistant staphylococcus aureus isolates had previously shown that in about half of the strains, the mechanism of triclosan resistance consists on the heterologous duplication of the triclosan target gene due to the acquisition of an additional fabi allele ...201627446047
transfer of mupirocin resistance from staphylococcus haemolyticus clinical strains to staphylococcus aureus through conjugative and mobilizable plasmids.coagulase-negative staphylococci are thought to act as reservoirs of antibiotic resistance genes that can be transferred to staphylococcus aureus, thus hindering the combat of this bacterium. in this work, we analyzed the presence of plasmids conferring resistance to the antibiotic mupirocin-widely used to treat and prevent s. aureus infections in hospital environments-in nosocomial s. haemolyticus strains. about 12% of the 75 strains tested were resistant to mupirocin, and this phenotype was co ...201627190144
carriage of meticillin-resistant staphylococci between humans and animals on a small farm.meticillin-resistant staphylococci (mrs) are pathogens of increasing importance to human and animal health worldwide. transmission of meticillin-resistant staphylococcus aureus (mrsa) between animals and humans has been well documented. by contrast, information about transmission of other staphylococcus spp. is limited.201627188770
antimicrobial activity of ceftaroline and comparator agents when tested against numerous species of coagulase-negative staphylococcus causing infection in us hospitals.a total of 1593 coagulase-negative staphylococci (cons) considered clinically significant were collected from 71 us medical centers in 2013-2014 and tested for susceptibility by clsi broth microdilution methods. species identification was performed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. overall, 59.7% of isolates were oxacillin resistant (mrcons). ceftaroline (mic50/90, 0.25/0.5μg/ml) inhibited 99.2% of cons at ≤1μg/ml (susceptible breakpoint for staphyl ...201626971182
trends and molecular mechanisms of antimicrobial resistance in clinical staphylococci isolated from companion animals over a 16 year period.the objective of this study was to investigate the evolution of resistance to antimicrobials, corresponding mechanisms and molecular characteristics of staphylococcus spp., between 1999 and 2014.201626944924
characterization of staphylococci in urban wastewater treatment plants in spain, with detection of methicillin resistant staphylococcus aureus st398.the objective of this study was to determine the prevalence of staphylococcus in urban wastewater treatment plants (uwtp) of la rioja (spain), and to characterize de obtained isolates. 16 wastewater samples (8 influent, 8 effluent) of six uwtps were seeded on mannitol-salt-agar and oxacillin-resistance-screening-agar-base for staphylococci and methicillin-resistant staphylococcus aureus recovery. antimicrobial susceptibility profile was determined for 16 antibiotics and the presence of 35 antimi ...201626840519
[distribution and drug sensitivity test of bacteria of patients on chronic rhinosinusitis with or without nasal polyps].to study the distribution and drug sensitivity test of bacteria of patients on chronic rhinosinusitis with or without nasal polyps.201627192905
redefining healthy urine: a cross-sectional exploratory metagenomic study of people with and without bladder dysfunction.we used the pathoscope platform to perform species level analyses of publicly available, 16s rrna pyrosequenced, asymptomatic urine data to determine relationships between microbiomes, and clinical and functional phenotypes.201626807926
[effects of antibiotic stewardship on neonatal bloodstream infections].to investigate the effects of antibiotic stewardship on the pathogen and clinical outcome of neonatal bloodstream infections (bsis).201627655532
staphylococcus simulans recombinant lysostaphin: production, purification, and determination of antistaphylococcal activity.staphylococcus simulans lysostaphin is an endopeptidase lysing staphylococcus cell walls by cleaving pentaglycine cross-bridges in their peptidoglycan. a synthetic gene encoding s. simulans lysostaphin was cloned in escherichia coli cells, and producer strains were designed. the level of produced biologically active lysostaphin comprised 6-30% of total e. coli cell protein (depending on e. coli m15 or bl21 producer) under batch cultivation conditions. new methods were developed for purification ...201627297900
association of diverse bacterial communities in human bile samples with biliary tract disorders: a survey using culture and polymerase chain reaction-denaturing gradient gel electrophoresis methods.bacterial infection is considered a predisposing factor for disorders of the biliary tract. this study aimed to determine the diversity of bacterial communities in bile samples and their involvement in the occurrence of biliary tract diseases. a total of 102 bile samples were collected during endoscopic retrograde cholangiopancreatography (ercp). characterization of bacteria was done using culture and polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) methods. antimicro ...201627193890
[clinical analysis for patients with continuous ambulatory peritoneal dialysis associated peritonitis].to analyze the clinical characteristics of continuous ambulatory peritoneal dialysis (capd) associated peritonitis in the tertiary hospitals and to discuss the preventive and therapeutic strategy.
 methods: the clinical characteristics, pathogens, resistance and outcomes of 126 capd associated peritonitis in 104 patients from jan, 2013 to june, 2016, were retrospectively analyzed.
 results: among the patients, the incidence rates of abdominal pain, fever, diarrhea and emesis were 104 (82.54%), 5 ...201628070047
cytokine and microbial profiles in relation to the clinical outcome following treatment of peri-implantitis.to study whether cytokine levels and bacterial counts in p atients with peri-implantitis reflect clinical treatment outcome following non-surgical management.201627422156
semen collection methods affect the bacterial composition of post-thawed semen of silver barb (barbodes gonionotus).biosafety issue associated with the risk of pathogenic contamination of cryopreserved semen is a common concern because of associated declines in sperm quality, storage period and disease transmission. this study was conducted to evaluate the effects of methods of semen collection on sperm quality and bacterial composition of post-thawed semen of silver barb (barbodes gonionotus). semen collection methods consisted of four treatments: (1) hand-stripping of abdomen without rinsing of urogenital a ...201626778122
validation of the bact/alert(r) 3d system for rapid sterility testing of biopharmaceutical samples.the bact/alert® 3d system was validated to determine the sterility of different types of biopharmaceutical samples such as water for injection, unprocessed bulk, and finished bulk. the installation, operation, and performance qualification were completed and verified under good manufacturing practices. during the installation and operation validation stages, the functionality and security of the system and software were completed and verified. for the performance qualification, 11 microorganisms ...201622307661
assessment of the bacterial contamination of hand air dryer in washrooms.the present study was carried out, using standard techniques, to identify and count the bacterial contamination of hand air dryers, used in washrooms. bacteria were isolated from the air flow, outlet nozzle of warm air dryers in fifteen air dryers used in these washrooms. bacteria were found to be relatively numerous in the air flows. bacterially contaminated air was found to be emitted whenever a warm air dryer was running, even when not being used for hand drying. our investigation shows that ...201626981009
isolation and characterization of two novel psychrotrophic decabromodiphenyl ether-degrading bacteria from river sediments.decabromodiphenyl ether (bde-209) is a brominated flame retardant and a priority contaminant. currently, little information is available about its significance in the environment, specifically about its susceptibility to aerobic biotransformation at low temperature. in this work, five phylogenetically diverse bde-209-degrading bacterial strains were isolated from river sediments of northern china. these strains were distributed among four different genera-acinetobacter, pseudomonas, bacillus and ...201626517991
bacterial contamination of platelet products in the blood transfusion center of isfahan, iran.aim: overall the risk of transfusion transmitted infections has decreased, especially viral infections like hiv and hepatitis b and c. bacterial contamination of blood and its cellular components, however, remains a common microbiological cause of transfusion associated morbidity and mortality. platelets pose a special risk given their preservation methods. the incidence of these episodes needs to be assessed and updated on regular basis to accurately manage the risk of transfusion transmitted b ...201628066700
clinical experiences with permanent demand pacemakers. 20165100990
[complications and reoperation in reconstructive surgery of the peripheral arteries]. 20165037232
experimental lamellar corneal grafting in buffalo calves (bos bubalis). 20164923244
effect of combination treatment with cyclophosphamide and isogeneic or allogeneic spleen and bone marrow cells in leukemic (l1210) mice. 20164925903
[75th birthday of prof. dr. aleksander kostić]. 20164911485
[chronic dialysis and kidney transplantation]. 20164893552
inhibition of the in vitro secretion of insulin by an extract of pancreatic alpha-1 cells. 20164889114
[electrocardiography in primary cardiomyopathy]. 20164889697
[edward flatau]. 20164896412
enhancement of antibody synthesis in vitro by mercaptoethanol. 20165061825
lymphography in mycosis fungoides. 20164834169
profile of active and terminated patients in a methadone maintenance program. 20164808230
symposium: the children's apperception test: its use in developmental assessments of normal children. introduction. 20164879249
no evidence for tissue-specific sequences of cytoplasmic 5 s and 5.8 s ribosomal rna's in the broad bean. 20164744398
[results with roux's y-anastomosis]. 20164739597
preparation of electroendosmosis-free agarose gel and exemplification of its use in crossed immunoelectrophoresis. 20164750692
isoniazid toxicity: reports of lactic acidosis and keratitis. 20165101723
[paroxysmal cold hemoglobinuria]. 20165093392
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