Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| Denitrification capacity of a landfilled refuse in response to the variations of COD/NO3--N in the injected leachate. | Effects of different chemical oxygen demand (COD) to nitrate concentration ratios in the injected leachate on the denitrification capacity of landfilled municipal solid waste were evaluated. Results showed that the 6-year-old refuse possessed high denitrification capacity. The nitrate reduction rate increased with the increasing COD concentration in the injected leachate. When the initial COD concentration increased to 6500 mg l(-1), nitrate reduction rate could reach up to 6.85 mg NO3--N l(-1) ... | 2012 | 22071241 |
| microbial community analysis in the autotrophic denitrification process using spent sulfidic caustic by denaturing gradient gel electrophoresis of pcr-amplified genes. | spent sulfidic caustic (ssc) produced from petrochemical plants contains a high concentration of hydrogen sulfide and alkalinity, and some almost non-biodegradable organic compounds such as benzene, toluene, ethylbenzene and xylenes (btex). ssc is mainly incinerated with auxiliary fuel, leading to secondary pollution problems. the reuse of this waste is becoming increasingly important from economic and environmental viewpoints. to denitrify wastewater with low cod/n ratio, additional carbon sour ... | 2011 | 21278470 |
| [experimental study of autotrophic denitrification bacteria through bioaugmentation of activated sludge from municipal wastewater plant]. | activated sludge of municipal wastewater treatment plant was domesticated by sulfur as the electron donor under autotrophic. the sludge activity was determined by measuring growth rate of sludge. the removal efficiency of nitrate and sulfate production efficiency were analyzed by continuously measuring the concentration of no3(-)-n and so4(2-). when the removal efficiency of nitrate was more than 90%, 16s rrna genetic libraries were built up to compare their microbial biodiversity. the growth ra ... | 2011 | 21717771 |
| development of bacterial community during spontaneous succession on spoil heaps after brown coal mining. | changes in the abundance of bacteria and fungi and in the composition of bacterial communities during primary succession were investigated in a brown coal mine deposit area near sokolov, the czech republic, using phospholipid fatty acids analysis, microarray and 16s rrna gene sequencing. the study considered a chronosequence of sites undergoing spontaneous succession: 6-, 12-, 21- and 45-year-old and a 21-year-old site revegetated with alnus glutinosa. during succession, organic carbon and the t ... | 2011 | 21707674 |
| Phylogenetic assessment of culture collection strains of Thiobacillus thioparus, and definitive 16S rRNA gene sequences for T. thioparus, T. denitrificans, and Halothiobacillus neapolitanus. | The 16S rRNA gene sequences of 12 strains of Thiobacillus thioparus held by different culture collections have been compared. A definitive sequence for the reference type strain (Starkey; ATCC 8158(T)) was obtained. The sequences for four examples of the Starkey type strain were essentially identical, confirming their sustained identity after passage through different laboratories. One strain (NCIMB 8454) was reassigned as a strain of Halothiobacillus neapolitanus, and a second (NCIMB 8349) was ... | 2011 | 21858648 |
| anaerobic, nitrate-dependent oxidation of pyrite nanoparticles by thiobacillus denitrificans. | pyrite is a key mineral in the global biogeochemical cycles of sulfur and iron, yet its anaerobic microbial oxidation has eluded geochemists and microbiologists for decades. recent reports indicated that anaerobic oxidation of pyrite is occurring, but the mechanism remains unclear. here, we provide evidence for the capability of <i>thiobacillus denitrificans</i> to anaerobically oxidize a putatively nanosized pyrite particle fraction with nitrate as an electron acceptor. nanosized pyrite was re ... | 2011 | 22142180 |
| acidiferrobacter thiooxydans, gen. nov. sp. nov.; an acidophilic, thermo-tolerant, facultatively anaerobic iron- and sulfur-oxidizer of the family ectothiorhodospiraceae. | a comprehensive physiological and phylogenetic characterisation was carried out of "thiobacillus ferrooxidans" m-1, an acidophilic iron-oxidizing bacterium first described over 25 years ago. phylogenetically, strain m-1 is a gammaproteobacterium, most closely related to alkaliphilic ectothiorhodospira spp. and only distantly to iron-oxidizing acidithiobacilli. physiological examination confirmed that strain m-1 can grow autotrophically not only by ferrous iron oxidation but also, in contrast to ... | 2011 | 21311931 |
| bacterial community composition characterization of a lead-contaminated microcoleus sp. consortium. | a microcoleus sp. consortium, obtained from the ebro delta microbial mat, was maintained under different conditions including uncontaminated, lead-contaminated, and acidic conditions. | 2011 | 21340467 |
| enhancement of copper availability and microbial community changes in rice rhizospheres affected by sulfur. | the role of sulfur on the availability of cu and the bacterial community in rice rhizospheres was investigated by pot experiments. with sulfur addition, ph in rhizosphere soil decreased and mg(no3)2 extractable cu increased significantly. the bacterial community composition also changed with sulfur addition. some specific clones having high similarity to thiobacillus, which indicated that sulfur oxidation in the rice rhizosphere could increase the availability of cu. these results suggested that ... | 2011 | 21350394 |
| effects of sulphur and thiobacillusthioparus on cow manure aerobic composting. | a simulated aerobic composting experiment was used to explore the effects of sulphur and thiobacillusthioparus during six manure composting treatments. the addition of sulphur led to a decrease of the ph level within the range 6-6.3, which was lower than the control treatment (ck). the concentration of ammonium nitrogen in t1 (0.25% sulphur), t2 (0.5% sulphur), t3 (0.25% sulphur+t. thioparus) and t4 (0.5% sulphur+t. thioparus) were much higher than the ammonium n in ck. the results indicated tha ... | 2011 | 21482106 |
| microbial community structure and dynamics in a mixotrophic nitrogen removal process using recycled spent caustic under different loading conditions. | a laboratory-scale bardenpho process was established to investigate the proper nitrogen loading rate (nlr) when modified spent caustic (msc) is applied as electron donor and alkalinity source for denitrification. msc injection induced autotrophic nitrogen removal with sulfur as electron donor and heterotrophic denitrification. the nitrogen removal rate (nrr) did not increase proportionally to nlr. based on the total nitrogen concentration in the effluent observed in the trials with msc, the nlr ... | 2011 | 21601446 |
| [regulation of hydrogen sulfide level by acidophobic bacteria of thiobacillus genus in technogenic reservoirs of sulfur mining regions]. | an increase of acidophobic thione bacteria quantity in rozdil and yavoriv reservoirs of sulfur mining regions during 2005-2009 years, which correlates with a decrease of hydrogen sulfide content in water surface layers, was shown. the ability of acidophobic bacteria of thiobacillus genus, isolated from "yavorivske" lake, to oxidize effectively hydrogen sulfide added into beijerinck medium instead of thiosulfate, was discovered. it was established, that hydrogen sulfide oxidizing efficiency by th ... | 2010 | 21381314 |
| biological oxidation of hydrogen sulfide in mineral media using a biofilm airlift suspension reactor. | hydrogen sulfide (h(2)s) removal in mineral media using thiobacillus thioparus tk-1 in a biofilm airlift suspension reactor (bas) was investigated to evaluate the relationship between biofilm formation and changes in inlet loading rates. aqueous sodium sulfide was fed as the substrate into the continuous bas-reactor. the reactor was operated at a constant temperature of 30 degrees c and a ph of 7, the optimal temperature and ph for biomass growth. the startup of the reactor was performed with ba ... | 2010 | 20594822 |
| [characteristic of autotrophic denitrification in bioaugmented anaerobic system]. | an autotrophic denitrification process using reduced sulfide as electron donor in anaerobic system is proposed as an efficient and cost effective alternative to conventional heterotrophic denitrification for low c/n ratio wastewaters and for simultaneous removal of sulfide and nitrate. a suspended culture of thiobacillus denitrificans bacteria was fast and efficiently established in anaerobic system. 5 series bioaugmented anaerobic systems with different n/s ratio synthetic influent were set up. ... | 2010 | 20623859 |
| [biofilm on a metal surface as a factor of microbial corrosion]. | main attention was given in the present review to the research methods, phases of biofilm's forming, exopolymer compounds of bacteria as main biofilm forming factor. a microbial corrosion as a result of interaction between the biofilm and metal surface was considered. the interaction was displayed in biomineralization. the future trends of biofilms study were bound with research of their architecture. that architecture was determined by the structure and function of biofilms compounds: biopolyme ... | 2010 | 20695231 |
| [domestication study about desulfuration microorganism from oxidation ditch by low concentration so2]. | an excellent desulfuration microorganism with a quick growth and propagation, high activation, high efficiency of removing so2 is obtained from oxidation ditch of a city sewage treatment plant by inductive acclimatization over 6 d with low concentration so2 gas (100-2 000 mg/m3). the desulfurition microorganism get their energy sources for growth from transforming so2 (so3(2-)) to so4(2-). the predominant bacterium of the desulfuration microorganism has the same characteristic with thiobacillus ... | 2010 | 20698284 |
| postbiostimulation microbial community structure changes that control the reoxidation of uranium. | this study evaluated the influence of changes in the microbial community structure on reoxidation of reduced uranium during a postbiostimulation period. effluent groundwater from acetate-stimulated sediment flow-through columns was analyzed over 60 days after acetate amendment was discontinued. only a small reoxidation of iron or uranium (17%) occurred in the presence of 1-2 mg l(-1) o(2) influent groundwater for the 2-month period. most uranium reoxidation occurred during the first 2 weeks afte ... | 2010 | 20707815 |
| sulfur-driven autotrophic denitrification: diversity, biochemistry, and engineering applications. | sulfur-driven autotrophic denitrification refers to the chemolithotrophic process coupling denitrification with the oxidation of reduced inorganic sulfur compounds. ever since 1904, when thiobacillus denitrificans was isolated, autotrophic denitrifiers and their uncultured close relatives have been continuously identified from highly diverse ecosystems including hydrothermal vents, deep sea redox transition zones, sediments, soils, inland soda lakes, etc. currently, 14 valid described species wi ... | 2010 | 20809074 |
| characteristics of nitrogen removal and microbial distribution by application of spent sulfidic caustic in pilot scale wastewater treatment plant. | since spent sulfidic caustic (ssc) produced from petrochemical industry contains a high concentration of alkalinity and sulfide, it was expected that ssc could be used as an electron donor for autotrophic denitrification. to investigate the nitrogen removal performance, a pilot scale bardenpho process was operated. the total nitrogen removal efficiency increased as ssc dosage increased, and the highest efficiency was observed as 77.5% when ssc was injected into both anoxic tank (1) and (2). fish ... | 2010 | 20861561 |
| [effects of sulfur on microbial activity and community structure in a lead-polluted paddy soil]. | a pot experiment was conducted to study the microbial activity and community structure in a lead-polluted paddy soil under effects of amendment with different concentration sodium thiosulfate. the amendment of the sulfur-containing substrate increased the soil oxidation-reduction potential and respiration rate, promoted the growth of soil sulfur-oxidizing bacteria, and induced some changes in soil microbial community structure. the clone sequencing indicated that the specific bands of soil micro ... | 2010 | 20879544 |
| response of nitrifying bacterial communities to the increased thiocyanate concentration in pre-denitrification process. | changes in process performance and the nitrifying bacterial community associated with an increase of thiocyanate (scn-) loading were investigated in a pre-denitrification process treating industrial wastewater. the increased scn- loading led to the concentration of total nitrogen (tn) in the final effluent, but increasing the internal recycling ratio as an operation parameter from 2 to 5 resulted in a 21% increase in tn removal efficiency. in the aerobic reactor, we found that the nitrosomonas e ... | 2010 | 20933392 |
| bacteria involved in sulfur amendment oxidation and acidification processes of alkaline 'alperujo' compost. | eight strains of sulfur oxidizing bacteria were isolated from alkaline 'alperujo' compost, seven being identified as paracoccus thiocyanatus and one as halothiobacillus neapolitanus. this was the first time that p. thiocyanatus was isolated from mature compost. acidification capability of isolated strains was compared with type strains h. neapolitanus cip104769, thiobacillus denitrificans cip104767 and thiomonas intermedia cip104401. indigenous p. thiocyanatus strains were as much as or more eff ... | 2010 | 20970324 |
| sulfide and ammonium oxidation, acetate mineralization by denitrification in a multipurpose uasb reactor. | the physiological and kinetic behavior of a denitrifying granular sludge exposed to different sulfide loading rates (55-295 mg/l d) were evaluated in a uasb reactor fed with acetate, ammonium and nitrate. at any sulfide loading rates, the consumption efficiencies of sulfide, acetate and ammonium were above 95%, while nitrate consumption efficiencies were around 62-72%. at the highest sulfide loading rate the ammonium was used as electron donor for n(2) production. the increase of sulfide loading ... | 2010 | 21146980 |
| biofiltration of reduced sulphur compounds and community analysis of sulphur-oxidizing bacteria. | the present work aims to use a two-stage biotrickling filters for simultaneous treatment of hydrogen sulphide (h(2)s), methyl mercaptan (mm), dimethyl sulphide (dms) and dimethyl disulphide (dmds). the first biofilter was inoculated with acidithiobacillus thiooxidans (bat) and the second one with thiobacillus thioparus (btt). for separate feeds of reduced sulphur compounds (rsc), the elimination capacity (ec) order was dmds>dms>mm. the ec values were 9.8 g(mm-s)/m(3)/h (btt; 78% removal efficien ... | 2010 | 21216139 |
| effect of thiobacillus, sulfur, and vermicompost on the water-soluble phosphorus of hard rock phosphate. | sulfur, organic matter, and inoculation with sulfur-oxidizing bacteria are considered as amendments to increase the availability of phosphorus from rock phosphate. the present study was conducted to evaluate the best combination of sulfur, vermicompost, and thiobacillus thiooxidans inoculation with rock phosphate from yazd province for direct application to agricultural lands in iran. for such study, an experiment was carried out in a completely randomized design with factorial arrangement: elem ... | 2010 | 19736005 |
| linking performance to microbiology in biofilters treating dimethyl sulphide in the presence and absence of methanol. | the performance and microbiology of two inorganic biofilters treating dimethyl sulphide (dms) in the presence and absence of methanol was investigated. addition of methanol was shown to result in an increase in dms removal for methanol loadings below 90 g meoh per cubic metre per hour with the optimal methanol loading around 10-15 g meoh per cubic metre per hour for a dms loading of 3.4 g dms per cubic metre per hour, a fivefold increase in the dms removal rate compared to the biofilter treating ... | 2010 | 19838699 |
| protonation of type-1 cu bound histidines: a quantum chemical study. | the protonation of the solvent-exposed histidine ligands of the type-1 cu sites in five proteins, thiobacillus ferrooxidans rusticyanin, pseudomonas aeruginosa azurin, fern plastocyanin, alcaligenes faecalis pseudoazurin, and paracoccus versutus amicyanin, were studied with quantum chemical methods and conductorlike polarizable continuum model (cpcm). active site model molecules consisting of approximately 140 atoms were extracted from x-ray crystal structures and optimized with the homogeneous ... | 2010 | 20000723 |
| sulfuricella denitrificans gen. nov., sp. nov., a sulfur-oxidizing autotroph isolated from a freshwater lake. | a novel facultatively anaerobic, sulfur-oxidizing bacterium, strain skb26(t), was isolated from anoxic water of a freshwater lake in japan. the cells were rod-shaped, motile and gram-negative. strain skb26(t) oxidized elemental sulfur and thiosulfate to sulfate as sole energy sources. strain skb26(t) was microaerobic and could also utilize nitrate as an electron acceptor, reducing it to nitrogen. growth was observed at temperatures below 28 °c; optimum growth was observed at 22°c. the ph range f ... | 2010 | 20081014 |
| bacterial communities associated with the rhizosphere of pioneer plants (bahia xylopoda and viguiera linearis) growing on heavy metals-contaminated soils. | in this study, the bacterial communities associated with the rhizospheres of pioneer plants bahia xylopoda and viguiera linearis were explored. these plants grow on silver mine tailings with high concentration of heavy metals in zacatecas, mexico. metagenomic dnas from rhizosphere and bulk soil were extracted to perform a denaturing gradient gel electrophoresis analysis (dgge) and to construct 16s rrna gene libraries. a moderate bacterial diversity and twelve major phylogenetic groups including ... | 2010 | 20084459 |
| polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent. | biological purification processes are effective tools in the treatment of hazardous wastes such as toxic compounds produced in coal coking. in this study, the microbial community of a lab-scale activated sludge system treating coking effluent was assessed by cultivation-based (strain isolation and identification, biodegradation tests) and culture-independent techniques (sequence-aided t-rflp, taxon-specific pcr). the results of the applied polyphasic approach showed a simple microbial community ... | 2010 | 20093025 |
| analysis of iron- and sulfur-oxidizing bacteria in a treatment plant of acid rock drainage from a japanese pyrite mine by use of ribulose-1, 5-bisphosphate carboxylase/oxygenase large-subunit gene. | iron- and sulfur-oxidizing bacteria in a treatment plant of acid rock drainage (ard) from a pyrite mine in yanahara, okayama prefecture, japan, were analyzed using the gene (cbbl) encoding the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (rubisco). analyses of partial sequences of cbbl genes from acidithiobacillus ferrooxidans, acidithiobacillus thiooxidans and acidithiobacillus caldus strains revealed the diversity in their cbbl gene sequences. in contrast to the presence o ... | 2010 | 20159572 |
| functional roles of the aromatic residues in the stabilization of the [fe(4)s(4)] cluster in the iro protein from acidithiobacillus ferrooxidans. | the iro protein is a member of hipip family with the [fe(4)s(4)] cluster for electron transfer. many reports proposed that the conserved aromatic residues might be responsible for the stability of the iron-sulfur cluster in hipip. in this study, tyr10 was found to be a critical residue for the stability of the [fe(4)s(4)] cluster according to site-directed mutagenesis results. tyr10, phe26 and phe48 were essential for the stability of the [fe(4)s(4)] cluster under acidic condition. trp44 were no ... | 2010 | 20208432 |
| direct current stimulation of thiobacillus ferrooxidans bacterial metabolism in a bioelectrical reactor without cation-specific membrane. | a bioelectrical reactor without cation-specific membrane was designed to test effects of direct electrical current on growth of thiobacillus ferrooxidans bacterium. the results indicated that the cell significantly enhanced the growth of t. ferrooxidans. at a current of 30 ma, the maximum cells density reached 1.39 x 10(9)cells/ml within 84 h, which was 10 times faster than under a conventional cultivation method, in which electrical current is not used. a lag phase during the growth of t. ferro ... | 2010 | 20227275 |
| investigation of bacterial diversity in membrane bioreactor and conventional activated sludge processes from petroleum refineries using phylogenetic and statistical approaches. | bacterial diversity of two distinct wastewater treatment systems, conventional activated sludge (cas) and membrane bioreactor (mbr), of petroleum refineries were investigated through 16s rrna gene libraries. sequencing and phylogenetic analysis showed that the bacterial community composition of sludge samples was distinct between the two wastewater treatment systems. mbr clones belonged predominantly to class betaproteobacteria, represented mainly by genera thiobacillus and thauera, whereas cas ... | 2010 | 20372011 |
| isolation of sulfur reducing and oxidizing bacteria found in contaminated drywall. | drywall from china has been reported to release sulfur producing products which are corrosive to metals, result in noxious odors, and represent a significant health risk. it has been reported that these emissions produce medical symptoms such as respiratory or asthma type problems, sinusitis, gastrointestinal disorders, and vision problems in home owners and their household pets. we report here a method of identifying a causative agent for these emissions by sampling affected gypsum wallboard an ... | 2010 | 20386658 |
| dissimilatory reduction of nitrate in seawater by a methylophaga strain containing two highly divergent narg sequences. | methylophaga spp. are methylotrophs commonly associated with marine environments and have been defined as strict aerobic methylotrophs. they have been shown previously to represent 50-70% of the bacterial population in the biofilm of the methanol-fed denitrification reactor treating a large seawater aquarium at the montreal biodome. it was therefore surprising to find such a high concentration of methylophaga spp. in anoxic conditions. in this study, we showed by cultivation-independent and -dep ... | 2010 | 20393572 |
| treatment of leather industry wastewater by aerobic biological and fenton oxidation process. | degradation of leather industry wastewater by sole aerobic treatment incorporating thiobacillus ferrooxidans, fenton's reagents, and combined treatment was investigated in this study. the sole treatment by fenton's oxidation involving the introduction of 6g feso(4) and 266 g h(2)o(2) in a liter of wastewater at ph of 3.5 and 30 degrees c for 30 min at batch conditions reduced cod, bod(5), sulfide, total chromium and color up to 69%, 72%, 88%, 5%, 100% and t. ferrooxidans alone showed maximum red ... | 2010 | 20451322 |
| effects of nitrate on the stability of uranium in a bioreduced region of the subsurface. | the effects of nitrate on the stability of reduced, immobilized uranium were evaluated in field experiments at a u.s. department of energy site in oak ridge, tn. nitrate (2.0 mm) was injected into a reduced region of the subsurface containing high levels of previously immobilized u(iv). the nitrate was reduced to nitrite, ammonium, and nitrogen gas; sulfide levels decreased; and fe(ii) levels increased then deceased. uranium remobilization occurred concomitant with nitrite formation, suggesting ... | 2010 | 20527772 |
| bacterial community analysis of a gas-phase biotrickling filter for biogas mimics desulfurization through the rrna approach. | the bacterial composition of a lab-scale biotrickling filter (btf) treating high loads of h(2)s was investigated by the rrna approach. two 16s rrna gene clone libraries were established 42 and 189 d after reactor startup, while fluorescent in-situ hybridization (fish) with dna probes was performed throughout 260d of reactor operation. diversity, community structure and metamorphosis were studied from reactor startup to fully-established pseudo-steady state operation at near neutral ph and at an ... | 2010 | 20554311 |
| growth kinetics of hyphomicrobium and thiobacillus spp. in mixed cultures degrading dimethyl sulfide and methanol. | the growth kinetics of hyphomicrobium spp. and thiobacillus spp. on dimethyl sulfide (dms) and methanol (in the case of hyphomicrobium spp.) in an enrichment culture created from a biofilter cotreating dms and methanol were studied. specific growth rates of 0.099 h(-1) and 0.11 h(-1) were determined for hyphomicrobium spp. and thiobacillus spp., respectively, growing on dms at ph 7. these specific growth rates are double the highest maximum specific growth rate for bacterial growth on dms report ... | 2010 | 20562269 |
| self-organizing maps: a tool to ascertain taxonomic relatedness based on features derived from 16s rdna sequence. | exploitation of microbial wealth, of which almost 95% or more is still unexplored, is a growing need. the taxonomic placements of a new isolate based on phenotypic characteristics are now being supported by information preserved in the 16s rrna gene. however, the analysis of 16s rdna sequences retrieved from metagenome, by the available bioinformatics tools, is subject to limitations. in this study, the occurrences of nucleotide features in 16s rdna sequences have been used to ascertain the taxo ... | 2010 | 21289444 |
| dimethyl sulphide degradation using immobilized thiobacillus thioparus in a biotrickling filter. | gaseous dimethyl sulphide (dms) was eliminated in a biotrickling filter with thiobacillus thioparus grown in polyurethane foam cubes as carrier material. the temperature, ph and empty bed residence time of the gas were maintained at 30 degrees c, 7.0 and 40 s, respectively. in the first 45 days, dms loads of around 2.0 gdms m(-3) h(-1) were fed to the btf to adapt t. thioparus to dms consumption, attaining close to 100% removal efficiency (re) on day 46, and the maximum elimination capacity (ec) ... | 2009 | 19950469 |
| structure of the two-domain hexameric aps kinase from thiobacillus denitrificans: structural basis for the absence of atp sulfurylase activity. | the tbd_0210 gene of the chemolithotrophic bacterium thiobacillus denitrificans is annotated to encode a 60.5 kda bifunctional enzyme with atp sulfurylase and aps kinase activity. this putative bifunctional enzyme was cloned, expressed and structurally characterized. the 2.95 a resolution x-ray crystal structure reported here revealed a hexameric assembly with d(3) symmetry. each subunit contains a large n-terminal sulfurylase-like domain and a c-terminal aps kinase domain reminiscent of the two ... | 2009 | 19770499 |
| [aerobic and anaerobic degradation of thiocyanate by association of bacteria isolated from savage of metallurgical plant ]. | 2009 | 19827709 | |
| technological assessment of a mining-waste dump at the dexing copper mine, china, for possible conversion to an in situ bioleaching operation. | in order to extract copper metal from the waste dump of dexing copper mine and resolve the environmental problems caused by acidic water and heavy metals, a dump bioleaching plant was designed based on a series of experimental investigations. the investigation shown that the low-grade of the dump, refractoriness of chalcopyrite, leakage of pad, small acidithiobacillus population and low dump permeability are the main factors that contribute to the challenges faced by the plant. stability of the ... | 2009 | 19036579 |
| identification of c-type cytochromes involved in anaerobic, bacterial u(iv) oxidation. | anaerobic, bacterial reduction of water-soluble u(vi) complexes to the poorly soluble u(iv) mineral uraninite has been intensively studied as a strategy for in situ remediation of uranium-contaminated groundwater. a novel and potentially counteracting metabolic process, anaerobic, nitrate-dependent u(iv) oxidation, has recently been described in two bacterial species (geobacter metallireducens and thiobacillus denitrificans), but the underlying biochemistry and genetics are completely unknown. w ... | 2009 | 18470655 |
| nitrate removal by thiobacillus denitrificans immobilized on poly(vinyl alcohol) carriers. | nitrate contamination is becoming a widespread environmental problem, and autotrophic denitrification with thiobacillus denitrificans is a promising process considering efficiency, cost and maintenance. the denitrification efficiencies of t. denitrificans were compared in batch reactors between free cells and cells immobilized on polyvinyl alcohol (pva) carriers made with thrice freezing/thawing and boric acid methods. the results indicated that the free cell reactor of t. denitrificans added wi ... | 2009 | 18723283 |
| symmetrical refolding of protein domains and subunits: example of the dimeric two-domain 3-isopropylmalate dehydrogenases. | the refolding mechanism of the homodimeric two-domain 3-isopropylmalate dehydrogenase (ipmdh) from the organisms adapted to different temperatures, thermus thermophilus (tt), escherichia coli (ec), and vibrio sp. i5 (vib), is described. in all three cases, instead of a self-template mechanism, the high extent of symmetry and cooperativity in folding of subunits and domains have been concluded from the following experimental findings: the complex time course of refolding, monitored by trp fluores ... | 2009 | 19154118 |
| biological sulfide oxidation using autotrophic thiobacillus sp.: evaluation of different immobilization methods and bioreactors. | evaluation of various immobilization methods and bioreactors for sulfide oxidation using thiobacillus sp. was studied. | 2009 | 19187143 |
| a two-stage process for simultaneous thermophilic sludge digestion, pathogen control and metal leaching. | a consortium of aerobic sulphur oxidizing microorganisms native to municipal sewage sludge has been developed that is capable of reducing the ph of sludge from 7.0 to 2.0 under mild-thermophilic (45 degrees c) temperatures. this mild-thermophilic, acidophilic acclimatized consortium has then been used to develop a thermophilic acidophilic culture capable of reducing the ph of municipal sewage sludge from 7.0 to 4.8 at 60 degrees c. furthermore, these two cultures have been used to develop a nove ... | 2009 | 19213462 |
| thiobacillus thiophilus sp. nov., a chemolithoautotrophic, thiosulfate-oxidizing bacterium isolated from contaminated aquifer sediments. | strain d24tn(t) was enriched and isolated from sediment collected from a tar oil-contaminated aquifer at a former gasworks site located in duesseldorf-flingern, germany. cells of strain d24tn(t) were rod-shaped, non-spore-forming and stained gram-negative. thiosulfate was used as an electron donor. the organism was obligately chemolithoautotrophic and facultatively anaerobic, and grew with either oxygen or nitrate as electron acceptor. growth was observed at ph values between 6.3 and 8.7 and at ... | 2009 | 19244446 |
| ferrous iron oxidation and rusticyanin in halotolerant, acidophilic 'thiobacillus prosperus'. | the halotolerant acidophile 'thiobacillus prosperus' was shown to require chloride for growth. with ferrous iron as substrate, growth occurred at a rate similar to that of the well-studied acidophile acidithiobacillus ferrooxidans. previously, the salt (nacl) requirement of 't. prosperus' was not clear and its growth on ferrous iron was described as poor. a subtractive hybridization of cdnas from ferrous-iron-grown and sulfur-grown 't. prosperus' strain v6 led to identification of a cluster of g ... | 2009 | 19332831 |
| compared microbiology of granular sludge under autotrophic, mixotrophic and heterotrophic denitrification conditions. | water contamination by nitrate is a wideworld extended phenomena. biological autotrophic denitrification has a real potential to face this problem and presents less drawbacks than the most extended heterotrophic denitrification. three bench-scale uasb reactors were operated under autotrophic (r1, h2s as electron donor), mixotrophic (r2, h2s plus p-cresol as electron donors) and heterotrophic (r3, p-cresol as electron donor) conditions using nitrate as terminal electron acceptor. 16s rdna genetic ... | 2009 | 19342820 |
| characterization of the bacterial community in a biotrickling filter treating high loads of h(2)s by molecular biology tools. | the diversity and spatial distribution of bacteria in a lab-scale biotrickling filter treating high loads of hydrogen sulfide (h(2)s) were investigated. diversity and community structure were studied by terminal-restriction fragment length polymorphism (t-rflp). a 16s rrna gene clone library was established. near full-length 16s rrna gene sequences were obtained, and clones were clustered into 24 operational taxonomic units (otus). nearly 74% and 26% of the clones were affiliated with the phyla ... | 2009 | 19380998 |
| removal of hydrogen sulfide and ammonia from gas mixtures by co-immobilized cells using a new configuration of two biotrickling filters. | the simultaneous removal of h(2)s and nh(3) was investigated using two biotrickling filters packed with polyurethane foam cubes. one biotrickling filter was inoculated with thiobacillus thioparus atcc 23645 for the removal of h(2)s (btt) and the other filter with nitrosomonas europaea atcc 19718 for the removal of nh(3) (bne). three different configurations were studied by modification of the gas line and recirculation medium line. the best results were obtained with the bne biotrickling filter ... | 2009 | 19381001 |
| life without light: microbial diversity and evidence of sulfur- and ammonium-based chemolithotrophy in movile cave. | microbial diversity in movile cave (romania) was studied using bacterial and archaeal 16s rrna gene sequence and functional gene analyses, including ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco), soxb (sulfate thioesterase/thiohydrolase) and amoa (ammonia monooxygenase). sulfur oxidizers from both gammaproteobacteria and betaproteobacteria were detected in 16s rrna, soxb and rubisco gene libraries. dna-based stable-isotope probing analyses using 13c-bicarbonate showed that thiobacill ... | 2009 | 19474813 |
| removal of hydrogen sulfide by immobilized thiobacillus thioparus in a biotrickling filter packed with polyurethane foam. | in the work described here, a biotrickling filter with thiobacillus thioparus (atcc 23645) immobilized on polyurethane foam is proposed for the removal of hydrogen sulfide contained in air. the effect of surface velocity of the recirculation medium (5.9-1.2 m/h), sulfate concentration inhibition (3.0-10.7 g/l), ph (6.0-8.2), empty bed residence time (ebrt) (150-11 s) for constant loads of 11.5 and 2.9 g s/m(3)/h, and pressure drop of the system were investigated. the total amount of biomass immo ... | 2009 | 19501506 |
| ferrous iron production mediated by tetrathionate hydrolase in tetrathionate-, sulfur-, and iron-grown acidithiobacillus ferrooxidans atcc 23270 cells. | when tetrathionate-grown acidithiobacillus ferrooxidans atcc 23270 cells were incubated with ferric ions and tetrathionate at ph 3.0, ferrous ions were produced enzymatically. fe(3+)-reductase, which catalyzes fe(3+) reduction with tetrathionate, was purified to homogeneity not only from tetrathionate-grown, but also from sulfur- and iron-grown a. ferrooxidans atcc 23270 cells. the results for apparent molecular weight measured by sds-page (52.3 kd) and the n-terminal amino acid sequences of the ... | 2009 | 19502725 |
| analysis of bacteria communities in an up-flow fixed-bed (ufb) bioreactor for treating sulfide in hydrocarbon wastewater. | an up-flow fixed-bed (ufb) bioreactor with patented functional polyurethane foam (fpuf) carriers was used to treat sulfide in hydrocarbon wastewater. community compositions of autotrophic and heterotrophic bacteria were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). dgge results showed that a relatively stable bacterial community composed of heterotrophic and autotrophic bacteria formed in the bioreactor by the end of experiment, which ensured 92-100% s ... | 2009 | 19559604 |
| biochemistry and molecular biology of lithotrophic sulfur oxidation by taxonomically and ecologically diverse bacteria and archaea. | lithotrophic sulfur oxidation is an ancient metabolic process. ecologically and taxonomically diverged prokaryotes have differential abilities to utilize different reduced sulfur compounds as lithotrophic substrates. different phototrophic or chemotrophic species use different enzymes, pathways and mechanisms of electron transport and energy conservation for the oxidation of any given substrate. while the mechanisms of sulfur oxidation in obligately chemolithotrophic bacteria, predominantly belo ... | 2009 | 19645821 |
| preliminary x-ray crystallographic analysis of sulfide:quinone oxidoreductase from acidithiobacillus ferrooxidans. | the gene product of open reading frame afe_1293 from acidithiobacillus ferrooxidans atcc 23270 is annotated as encoding a sulfide:quinone oxidoreductase, an enzyme that catalyses electron transfer from sulfide to quinone. following overexpression in escherichia coli, the enzyme was purified and crystallized using the hanging-drop vapour-diffusion method. the native crystals belonged to the tetragonal space group p4(2)2(1)2, with unit-cell parameters a = b = 131.7, c = 208.8 a, and diffracted to ... | 2009 | 19652354 |
| kinetic properties of atp sulfurylase and aps kinase from thiobacillus denitrificans. | the thiobacillus denitrificans genome contains two sequences corresponding to atp sulfurylase (tbd_0210 and tbd_0874). both genes were cloned and expressed protein characterized. the larger protein (tbd_0210; 544 residues) possesses an n-terminal atp sulfurylase domain and a c-terminal aps kinase domain and was therefore annotated as a bifunctional enzyme. but, the protein was not bifunctional because it lacked atp sulfurylase activity. however, the enzyme did possess aps kinase activity and dis ... | 2009 | 19664586 |
| ecophysiology and the energetic benefit of mixotrophic fe(ii) oxidation by various strains of nitrate-reducing bacteria. | in order to assess the importance of nitrate-dependent fe(ii) oxidation and its impact on the growth physiology of dominant fe oxidizers, we counted these bacteria in freshwater lake sediments and studied their growth physiology. most probable number counts of nitrate-reducing fe(ii)-oxidizing bacteria in the sediment of lake constance, a freshwater lake in southern germany, yielded about 10(5) cells ml(-1) of the total heterotrophic nitrate-reducing bacteria, with about 1% (10(3) cells ml(-1)) ... | 2009 | 19732145 |
| effect of mycorrhizae, thiobacillus and sulfur nutrition on the chemical composition of soybean [glycine max (l.)] merr. seed. | a field experiment carried out in a calcareous soil with a low available phosphorus to evaluate effectiveness of biofertilizers, mycorrhizae (glomus intraradices) and thiobacillus sp. inoculation individually or in combination on seed yield, oil, protein and some elements (p, fe, mn, zn) concentration in two soybean [glycine max (l.) merr.] cultivars. the applied treatments were different fertilizers with 6 levels (including: np (control, 12 kg n ha(-1) as urea, 46 kg p2o5 ha(-1) as triple super ... | 2008 | 18814643 |
| hydrogen sulfide removal by thiobacillus thioparus bacteria on seashell bed biofilters. | the aim of this research is to achieve an efficient and cheap methods to remove h2s from the factories emissions. four serial cylinders are designed, 40 cm in height and 15 cm in diameter each. they are filled with bivalve seashells with 63% porosity which contains thiobacillus thioparus bacteria to the maximum height of 27.5 cm. by mixing phosphoric acid and sodium sulfide, h2s gas is released and its concentration is measured as mg m(-3) before injecting into the cylinders. a permanent measuri ... | 2008 | 18814657 |
| profiling of microbial communities in a bioreactor for treating hydrocarbon-sulfide-containing wastewater. | a technology of polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) was used to profile the structure and dynamic changes of microbial communities in a bioreactor for treating hydrocarbon-sulfide-containing (hsc) wastewater. the results showed that the heterotrophic genus of acinetobacter and the autotrophic genera of thiobacillus and thiomonas could survive well in all of three operating conditions. some special genera were also observed with changes of micro-ecoenviron ... | 2008 | 18817065 |
| evaluation of performance and microbial ecology of sequencing batch reactor and membrane bioreactor treating thin-film transistor liquid crystal display wastewater. | in taiwan, a substantial amount of thin-film transistor liquid crystal display (tft-lcd) wastewater is produced daily due to an increasing production of the opto-electronic industry in recent years. the main components of tft-lcd wastewater include dimethyl sulphoxide (dmso), monoethanolamine (mea), and tetra-methyl ammonium hydroxide (tmah), which are recognized as non-or slow-biodegradable organic compounds and limited information is available regarding their biological treatablility. this stu ... | 2008 | 18824808 |
| assessment of the addition of thiobacillus denitrificans and thiomicrospira denitrificans to chemolithoautotrophic denitrifying bioreactors. | the aim of the present study was to assess the impact of adding cultures of thiobacillus denitrificans and thiomicrospira denitrificans to two upflow anaerobic sludge bed (uasb) reactors: one inoculated with granular sludge and the other filled only with activated carbon (ac). the performances of the bioreactors and the changes in biomass were compared with a non-bioaugmented control uasb reactor inoculated with granular sludge. the reactors inoculated with granular sludge achieved efficiencies ... | 2008 | 18843596 |
| [cultivation and isolation of bacteria coupling nitrogen removal from wastewater and hydrogen sulfide removal from biogas]. | it was studied that during the startup of reactors the crucial bacteria coupling nitrogen removal from swine wastewater and hydrogen sulfide removal from biogas using bubbling absorption reactors without seeding sludge, with seeding anaerobic sludge and aerobic sludge, respectively. during the prophase of the experiment (before the 26th d), the removal rate of nitrogen and hydrogen sulfide were from 50% to 64% in reactors with seeding sludge, that in the reactor without seeding sludge was only 1 ... | 2008 | 18637368 |
| novel autotrophic arsenite-oxidizing bacteria isolated from soil and sediments. | arsenic oxidation is recognized as being mediated by both heterotrophic and chemoautotrophic microorganisms. enrichment cultures were established to determine whether chemoautotrophic microorganisms capable of oxidizing arsenite as(iii) to arsenate as(v) are present in selected contaminated but nonextreme environments. three new organisms, designated as strains ol-1, s-1 and cl-3, were isolated and found to oxidize 10 mm arsenite to arsenate under aerobic conditions using co2-bicarbonate (co2/hc ... | 2008 | 18717738 |
| a long-term performance test on an autotrophic denitrification column for application as a permeable reactive barrier. | the long-term performance of a sulfur-based reactive barrier system was evaluated using autotrophic denitrification in a large-scale column. a bacterial consortium, containing autotrophic denitrifiers attached on sulfur particles, serving as an electron donor, was able to transform 60 mg n l(-1) of nitrate into dinitrogen. in the absence of phosphate, the consortium was unable to remove nitrate, but after the addition of phosphate, nitrate removal was readily evident. once the column operation h ... | 2008 | 18722639 |
| microbial community analysis of two field-scale sulfate-reducing bioreactors treating mine drainage. | the microbial communities of two field-scale pilot sulfate-reducing bioreactors treating acid mine drainage (amd), luttrell and peerless jenny king (pjk), were compared using biomolecular tools and multivariate statistical analyses. the two bioreactors were well suited for this study because their geographic locations and substrate compositions were similar while the characteristics of influent amd, configuration and degree of exposure to oxygen were distinct. the two bioreactor communities were ... | 2008 | 18430021 |
| arsenic transformation and mobilization from minerals by the arsenite oxidizing strain wao. | analysis of arsenic concentrations in new jersey well water from the newark basin showed up to 15% of the wells exceed 10 microg l(-1), with a maximum of 215 microg l(-1). in some geologic settings in the basin, this mobile arsenic could be from the weathering of pyrite (fes2) found in black shale that contains up to 4% arsenic by weight. we hypothesized that under oxic conditions at circumneutral ph, the microbially mediated oxidation of sulfide in the pyrite lattice would lead to the release o ... | 2008 | 18441783 |
| biological treatment of toxic petroleum spent caustic in fluidized bed bioreactor using immobilized cells of thiobacillus rai01. | in the present studies, newly isolated thiobacillus sp was used for the treatment of synthetic spent sulfide caustic in a laboratory-scale fluidized bed bioreactor. the sulfide oxidation was tested using ca-alginate immobilized thiobacillus sp. initially, response surface methodology was applied for the optimization of four parameters to check the sulfide oxidation efficiency in batch mode. further, reactor was operated in continuous mode for 51 days at different sulfide loading rates and retent ... | 2008 | 18574569 |
| bacterial diversity in synovial fluids of patients with tmd determined by cloning and sequencing analysis of the 16s ribosomal rna gene. | the purpose of this study was to examine the presence of bacteria in synovial fluids from patients with temporomandibular joint disorder (tmd) by polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequencing. | 2008 | 18206402 |
| insights learned from pbtai1, a 229-kb accessory plasmid from bradyrhizobium sp. strain btai1 and prevalence of accessory plasmids in other bradyrhizobium sp. strains. | in silico, physiological and in planta analyses were used to characterize pbtai1, a 229-kb accessory plasmid from bradyrhizobium sp. strain btai1, and assess its potential ecological function under free-living and symbiotic growth conditions. sequence analysis revealed the presence of an uptake hydrogenase system, a repabc family plasmid replication module and open reading frames encoding type iv secretion system, trai and trar autoinducer proteins and several copper resistance-related proteins. ... | 2008 | 18219284 |
| molecular modeling of the ternary complex of rusticyanin-cytochrome c4-cytochrome oxidase: an insight to possible h-bond mediated recognition and electron transfer reaction in t. ferrooxidans. | the metabolism of thiobacillus ferrooxidans involves electron transfer from the fe+2 ions in the extracellular environment to the terminal oxygen in the bacterial cytoplasm through a series of periplasmic proteins like rusticyanin (rcy), cytochrome (cyt c4), and cytochrome oxidase (cco). the energy minimization and md studies reveal the stabilization of the three redox proteins in their ternary complex through the direct and water mediated h-bonds and electrostatic interaction. the surface expos ... | 2008 | 18282009 |
| comparison of bio-dissolution of spent ni-cd batteries by sewage sludge using ferrous ions and elemental sulfur as substrate. | bioleaching of spent ni-cd batteries using acidified sewage sludge was carried out in a continuous flow two-step leaching system including an acidifying reactor and a leaching reactor. two systems operated about 30d to achieve almost complete dissolution of heavy metals ni, cd and co in four ni-cd batteries. ferrous sulphate and elemental sulfur were used as two different substrates to culture indigenous thiobacilli in sewage sludge. ph and orp of the acidifying reactor was stabilized around 2.3 ... | 2008 | 17884135 |
| monitoring thiocyanate-degrading microbial community in relation to changes in process performance in mixed culture systems near washout. | changes in microbial community structure, associated with changes in process performance, were investigated with respect to the sludge retention time (srt) in bioreactors treating thiocyanate. among the seven reactors operated at 0.8-3.0 d srts, respectively, the reactor at 2.0 d srt displayed the maximal thiocyanate removal rate of 240.2mg/l/d. however, the thiocyanate removal efficiency suddenly decreased from 96.1% to 43.1% when the srt was reduced from 2.0 to 1.8d, corresponding to a 50.1% d ... | 2008 | 17935752 |
| microbial community dynamics in a chemolithotrophic denitrification reactor inoculated with methanogenic granular sludge. | denitrification is applied in the tertiary treatment of wastewater to reduce nitrogen pollution. fluorescence in situ hybridization (fish), catalyzed reporter deposition (card)-fish, cloning, and scanning electron microscopy (sem) were applied to follow the evolution of the microbial composition and structure of granular sludge in chemolithotrophic denitrifying bioreactors fed with nitrate and thiosulfate. fish oligonucleotide probes for the chemolitoautotrophic denitrifiers thiobacillus denitri ... | 2008 | 17689587 |
| bacterial community and biological nitrate removal: comparisons of autotrophic and heterotrophic reactors for denitrification with raw sewage. | an autotrophic denitrification reactor (adr-1) and a heterotrophic denitrification reactor (hdr-2) were operated to remove nitrate and nitrite in an anoxic environment in raw sewage. the no3-n removal rate of adr-1 was shown to range from 52.8% to 78.7%, which was higher than the no3-n removal rate of hdr-2. specific denitrification rates (sdnr) of adr-1 and hdr-2 were 3.0 to 4.0 and 1.1 to 1.2mgno3- n/gvss/h, respectively. from results of restriction fragment length polymorphism (rflp) of the 1 ... | 2008 | 19047828 |
| [isolation, identification and denitrification characterization of thiobacillus denitrificans]. | a autotrophic denitrifying bacterial strain, td, was isolated from soil and the strain was identified and characterized. the strain was gram negative, strictly chemolithoautotrophic, and short rod shaped bacterium. the 16s rdna sequence analysis revealed that strain td had a similarity of 99.85% with thiobacillus denitrificans. according to the morphologic, physiobiochemical characteristics and the analysis of its 16s rdna, the strain was identified as thiobacillus denitrificans. studies showed ... | 2008 | 19143397 |
| modeling study of rusticyanin-cytochrome c(4) complex: an insight to possible h-bond mediated recognition and electron--transfer process. | rusticyanin (rcy) mediated transfer of electron to cytochrome c(4) (cytc(4)) from the extracellular fe(+2) ion is primarily involved in the thiobacillus ferrooxidans induced bio-leaching of pyrite ore and also in the metabolism of this acidophilic bacteria. the modeling studies have revealed the two possible mode of rcy-cytc(4) complexation involving nearly the same stabilization energy approximately -15 x 10(3) kj/mol, one through n-terminal asp 15 and another -c terminal glu 121 of cytc(4) wit ... | 2007 | 17718594 |
| combined removal of sulfur compounds and nitrate by autotrophic denitrification in bioaugmented activated sludge system. | an autotrophic denitrification process using reduced sulfur compounds (thiosulfate and sulfide) as electron donor in an activated sludge system is proposed as an efficient and cost effective alternative to conventional heterotrophic denitrification for inorganic (or with low c/n ratio) wastewaters and for simultaneous removal of sulfide or thiosulfate and nitrate. a suspended culture of sulfur-utilizing denitrifying bacteria was fast and efficiently established by bio-augmentation of activated s ... | 2007 | 17724757 |
| isolation of methylophaga spp. from marine dimethylsulfide-degrading enrichment cultures and identification of polypeptides induced during growth on dimethylsulfide. | dimethylsulfide (dms)-degrading enrichment cultures were established from samples of coastal seawater, nonaxenic emiliania huxleyi cultures, and mixed marine methyl halide-degrading enrichment cultures. bacterial populations from a broad phylogenetic range were identified in the mixed dms-degrading enrichment cultures by denaturing gradient gel electrophoresis (dgge). sequences of dominant dgge bands were similar to those of members of the genera methylophaga and alcanivorax. several closely rel ... | 2007 | 17322322 |
| development of a genetic system for the chemolithoautotrophic bacterium thiobacillus denitrificans. | thiobacillus denitrificans is a widespread, chemolithoautotrophic bacterium with an unusual and environmentally relevant metabolic repertoire, which includes its ability to couple denitrification to sulfur compound oxidation; to catalyze anaerobic, nitrate-dependent oxidation of fe(ii) and u(iv); and to oxidize mineral electron donors. recent analysis of its genome sequence also revealed the presence of genes encoding two [nife]hydrogenases, whose role in metabolism is unclear, as the sequenced ... | 2007 | 17337560 |
| two-stage biofilter for effective nh3 removal from waste gases containing high concentrations of h2s. | a high h2s concentration inhibits nitrification when h2s and nh3 are simultaneously treated in a single biofilter. to improve nh3 removal from waste gases containing concentrated h2s, a two-stage biofilter was designed to solve the problem. in this study, the first biofilter, inoculated with thiobacillus thioparus, was intended mainly to remove h2s and to reduce the effect of h2s concentration on nitrification in the second biofilter, and the second biofilter, inoculated with nitrosomonas europa ... | 2007 | 17385601 |
| microbially-influenced degradation of solidified/stabilized metal waste. | in the present study, a refined microbially-influenced degradation method was used to evaluate the stability of a solidified synthetic waste containing chromium salt, cement and fly ash in two different proportions. the experimental samples showed evidence of microbial growth by leaching of sulfate. chromium leached by thiobacillus thiooxidans from the experimental samples 'c1' (10.26% crcl3 .6h2o; 89.74% cement) and 'fc1' (10.26% crcl3 .6h2o; 10% fly ash; 79.74% cement), after 30 days of exposu ... | 2007 | 17107784 |
| expression, purification and molecular modelling of the iro protein from acidithiobacillus ferrooxidans fe-1. | the iro protein was proposed to be involved in the iron respiratory electron transport chain in acidithiobacillus ferrooxidans, it is a member of hipip family with the iron-sulfur cluster for electron transfer. the gene of iro protein from a. ferrooxidans fe-1 was cloned and then successfully expressed in escherichia coli, finally purified by one-step affinity chromatography to homogeneity. the recombinant protein was observed to be dimer. the molecular mass of a monomer containing the [fe4s4] c ... | 2007 | 17140808 |
| succession of sulfur-oxidizing bacteria in the microbial community on corroding concrete in sewer systems. | microbially induced concrete corrosion (micc) in sewer systems has been a serious problem for a long time. a better understanding of the succession of microbial community members responsible for the production of sulfuric acid is essential for the efficient control of micc. in this study, the succession of sulfur-oxidizing bacteria (sob) in the bacterial community on corroding concrete in a sewer system in situ was investigated over 1 year by culture-independent 16s rrna gene-based molecular tec ... | 2007 | 17142362 |
| conserved water molecular dynamics of the different x-ray structures of rusticyanin: an unique aquation potentiality of the ligand bonded cu++ center. | the invariant water molecular interaction involving in the rusticyanin of thiobacillus ferrooxidans is thought to be important for its molecular complexation with other proteins at differential acidophilic situation. the comparative analysis of the different x-ray, energy minimized, and auto solvated structures of rusticyanin revealed the presence of five specific invariant bound water molecules (among the approximately 150 water molecules per monomer) in the crystals. the five w 205, w 206, w 1 ... | 2007 | 17206852 |
| structure of thiocyanate hydrolase: a new nitrile hydratase family protein with a novel five-coordinate cobalt(iii) center. | thiocyanate hydrolase (scnase) of thiobacillus thioparus thi115 is a cobalt(iii)-containing enzyme catalyzing the degradation of thiocyanate to carbonyl sulfide and ammonia. we determined the crystal structures of the apo- and native scnases at a resolution of 2.0 a. scnases in both forms had a conserved hetero-dodecameric structure, (alphabetagamma)(4). four alphabetagamma hetero-trimers were structurally equivalent. one alphabetagamma hetero-trimer was composed of the core domain and the betan ... | 2007 | 17222425 |
| thiocyanate hydrolase, the primary enzyme initiating thiocyanate degradation in the novel obligately chemolithoautotrophic halophilic sulfur-oxidizing bacterium thiohalophilus thiocyanoxidans. | thiohalophilus thiocyanoxidans is a first halophilic sulfur-oxidizing chemolithoautotrophic bacterium capable of growth with thiocyanate as an electron donor at salinity up to 4 m nacl. the cells, grown with thiocyanate, but not with thiosulfate, contained an enzyme complex hydrolyzing thiocyanate to sulfide and ammonia under anaerobic conditions with carbonyl sulfide as an intermediate. despite the fact of utilization of the <<cos pathway>>, high cyanase activity was also detected in thiocyanat ... | 2007 | 17964868 |
| [effect of the biofilm biopolymers on the microbial corrosion rate of the low-carbon steel]. | the relationship between exopolymer's specific production, relative carbohydrate and protein content in the biofilm exopolymers of the pure and mixed thiobacillus thioparus and stenotrophomonas maltophilia cultures and their corrosion activity was studied. change of growth model of investigated cultures from plankton to biofilm led to an increase of specific exopolymer's production. in the biofilm formed by t. thioparus and s. maltophilia biofilm on the low-carbon steel surface one could observe ... | 2007 | 17977451 |
| molecular analysis of the distribution and phylogeny of the soxb gene among sulfur-oxidizing bacteria - evolution of the sox sulfur oxidation enzyme system. | the soxb gene encodes the soxb component of the periplasmic thiosulfate-oxidizing sox enzyme complex, which has been proposed to be widespread among the various phylogenetic groups of sulfur-oxidizing bacteria (sob) that convert thiosulfate to sulfate with and without the formation of sulfur globules as intermediate. indeed, the comprehensive genetic and genomic analyses presented in the present study identified the soxb gene in 121 phylogenetically and physiologically divergent sob, including s ... | 2007 | 17991026 |
| hydrogen sulfide removal by immobilized thiobacillus novellas on sio2 in a fluidized bed reactor. | the removal of hydrogen sulfide (h2s) from aqueous media was investigated using thiobacillus novellas cells immobilized on a sio2 carrier (biosand). the optimal growth conditions for the bacterial strain were 30 degrees c and initial ph of 7.0. the main product of hydrogen sulfide oxidation by t. novellus was identified as the sulfate ion. a removal efficiency of 98% was maintained in the three-phase fluidized-bed reactor, whereas the efficiency was reduced to 90% for the two-phase fluidized-bed ... | 2007 | 18051764 |
| investigation of solid-phase buffers for sulfur-oxidizing autotrophic denitrification. | this paper investigates biological denitrification using autotrophic microorganisms that use elemental sulfur as an electron donor. in this process, for each gram of nitrate-nitrogen removed, approximately 4.5 g of alkalinity (as calcium carbonate) are consumed. because denitrification is severely inhibited below ph 5.5, and alkalinity present in the influent wastewaters is less than the alkalinity consumed, an external buffer was needed to arrest any drop in ph from alkalinity consumption. a pa ... | 2007 | 18198697 |
| molecular analysis of the distribution and phylogeny of dissimilatory adenosine-5'-phosphosulfate reductase-encoding genes (aprba) among sulfur-oxidizing prokaryotes. | dissimilatory adenosine-5'-phosphosulfate (aps) reductase (aprba) is a key enzyme of the dissimilatory sulfate-reduction pathway. homologues have been found in photo- and chemotrophic sulfur-oxidizing prokaryotes (sop), in which they are postulated to operate in the reverse direction, oxidizing sulfite to aps. newly developed pcr assays allowed the amplification of 92-93 % (2.1-2.3 kb) of the aps reductase locus aprba. pcr-based screening of 116 taxonomically divergent sop reference strains reve ... | 2007 | 17906146 |
| [acidification treatment of woolscouring effluent by using acidophilic thiobacillus]. | suspended solids dispersed in woolscouring effluent (wse) could be flocculated and precipitated in low ph. the aim of this study is to decrease ph in wse, result in liquid-solid separation and remove cod of wse through bio-acidification of acidophilic thiobacillus. acidophilic thiobacillus ts6 and sulfur powder as energy substance were inoculated and added into wse for incubation in shaker at 180 r/min and 30 degrees c for 12 d. the results showed that suspended solids in wse would flocculate an ... | 2007 | 17489202 |
| nitrate stimulation of indigenous nitrate-reducing, sulfide-oxidising bacterial community in wastewater anaerobic biofilms. | the role of the nitrate-reducing, sulfide-oxidising bacteria (nr-sob) in the nitrate-mediated inhibition of sulfide net production by anaerobic wastewater biofilms was analyzed in two experimental bioreactors, continuously fed with the primary effluent of a wastewater treatment plant, one used as control (brc) and the other one supplemented with nitrate (brn). this study integrated information from h(2)s and ph microelectrodes, rna-based molecular techniques, and the time course of biofilm growt ... | 2007 | 17524444 |