Publications

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specific members of the predominant gut microbiota predict pouchitis following colectomy and ipaa in uc.pouchitis is the most common complication after colectomy with ileal pouch-anal anastomosis (ipaa) for uc and the risk is the highest within the 1st year after surgery. the pathogenesis is not completely understood but clinical response to antibiotics suggests a role for gut microbiota. we hypothesised that the risk for pouchitis can be predicted based on the faecal microbial composition before colectomy.201726423113
luminal contents from the gut of colicky infants induce visceral hypersensitivity in mice.the pathophysiology of infantile colic is poorly understood, though various studies report gut microbiota dysbiosis in colicky infants. we aimed to test the hypothesis that colic-related dysbiosis is associated with visceral hypersensitivity triggered by an altered luminal milieu.201727910234
functional characterization of a gene locus from an uncultured gut bacteroides conferring xylo-oligosaccharides utilization to escherichia coli.in prominent gut bacteroides strains, sophisticated strategies have been evolved to achieve the complete degradation of dietary polysaccharides such as xylan, which is one of the major components of the plant cell wall. polysaccharide utilization loci (puls) consist of gene clusters encoding different proteins with a vast arsenal of functions, including carbohydrate binding, transport and hydrolysis. transport is often attributed to tonb-dependent transporters, although major facilitator superfa ...201627573446
structural and mechanistic insights into a bacteroides vulgatus retaining n-acetyl-β-galactosaminidase that uses neighbouring group participation.bacteroides vulgatus is a member of the human microbiota whose abundance is increased in patients with crohn's disease. we show that a b. vulgatus glycoside hydrolase from the carbohydrate active enzyme family gh123, bvgh123, is an n-acetyl-β-galactosaminidase that acts with retention of stereochemistry, and, through a 3-d structure in complex with gal-thiazoline, provide evidence in support of a neighbouring group participation mechanism.201627546776
symbiotic gut commensal bacteria act as host cathepsin s activity regulators.cathepsin s (ctss) is a lysosomal protease whose activity regulation is important for mhc-ii signaling and subsequent activation of cd4(+) t cell mediated immune responses. dysregulation of its enzymatic activity or enhanced secretion into extracellular environments is associated with the induction or progression of several autoimmune diseases. here we demonstrate that commensal intestinal bacteria influence secretion rates and intracellular activity of host ctss and that symbiotic bacteria, i.e ...201627484364
human gut microbes impact host serum metabolome and insulin sensitivity.insulin resistance is a forerunner state of ischaemic cardiovascular disease and type 2 diabetes. here we show how the human gut microbiome impacts the serum metabolome and associates with insulin resistance in 277 non-diabetic danish individuals. the serum metabolome of insulin-resistant individuals is characterized by increased levels of branched-chain amino acids (bcaas), which correlate with a gut microbiome that has an enriched biosynthetic potential for bcaas and is deprived of genes encod ...201627409811
extensive mobilome-driven genome diversification in mouse gut-associated bacteroides vulgatus mpk.like many other bacteroides species, bacteroides vulgatus strain mpk, a mouse fecal isolate which was shown to promote intestinal homeostasis, utilizes a variety of mobile elements for genome evolution. based on sequences collected by pacific biosciences smrt sequencing technology, we discuss the challenges of assembling and studying a bacterial genome of high plasticity. additionally, we conducted comparative genomics comparing this commensal strain with the b. vulgatus type strain atcc 8482 as ...201627071651
expression of arsenic resistance genes in the obligate anaerobe bacteroides vulgatus atcc 8482, a gut microbiome bacterium.the response of the obligate anaerobe bacteroides vulgatus atcc 8482, a common human gut microbiota, to arsenic was determined. b. vulgatus atcc 8482 is highly resistant to pentavalent as(v) and methylarsenate (mas(v)). it is somewhat more sensitive to trivalent inorganic as(iii) but 100-fold more sensitive to methylarsenite (mas(iii)) than to as(iii). b. vulgatus atcc 8482 has eight continuous genes in its genome that we demonstrate form an arsenical-inducible transcriptional unit. the first ge ...201627040269
the evolution of cooperation within the gut microbiota.cooperative phenotypes are considered central to the functioning of microbial communities in many contexts, including communication via quorum sensing, biofilm formation, antibiotic resistance, and pathogenesis. the human intestine houses a dense and diverse microbial community critical to health, yet we know little about cooperation within this important ecosystem. here we test experimentally for evolved cooperation within the bacteroidales, the dominant gram-negative bacteria of the human inte ...201627111508
microbial community of healthy thai vegetarians and non-vegetarians, their core gut microbiota, and pathogen risk.pyrosequencing analysis of intestinal microflora from healthy thai vegetarians and non-vegetarians exhibited 893 otus covering 189 species. the strong species indicators of vegetarians and non-vegetarians were prevotella copri and bacteroides vulgatus as well as bacteria close to escherichia hermanii with % relative abundance of 16.9 and 4.5-4.7, respectively. core gut microbiota of the vegetarian and non-vegetarian groups consisted of 11 and 20 different bacterial species, respectively, belongi ...201627381339
distinctly altered gut microbiota in the progression of liver disease.recent studies underscore important roles of intestinal microbiota and the bacterial lipopolysaccharides (lps) production in the pathogenesis of liver disease. however, how gut microbiota alters in response to the development of steatosis and subsequent progression to nonalcoholic steatohepatitis (nash) and hepatocellular carcinoma (hcc) remains unclear. we aimed to study the gut microbial changes over liver disease progression using a streptozotocin-high fat diet (stz-hfd) induced nash-hcc c57b ...201627036035
identification of vaginal fluid, saliva, and feces using microbial signatures in a han chinese population.in recent years, forensic scientists have focused on the discrimination of body fluids using microbial signatures. in this study, we performed pcr-based detection of microbial signatures of vaginal fluid, saliva, and feces in a han chinese population. we investigated the 16s rrna genes of lactobacillus crispatus, lactobacillus gasseri, lactobacillus jensenii, lactobacillus iners, and atopobium vaginae in vaginal fluid, the 16s rrna and the glucosyltransferase enzyme genes of streptococcus saliva ...201627570236
imbalance of bacteriome profiles within the finnish diabetes prediction and prevention study: parallel use of 16s profiling and virome sequencing in stool samples from children with islet autoimmunity and matched controls.we set out to explore associations between the stool bacteriome profiles and early-onset islet autoimmunity, taking into account the interactions with the virus component of the microbiome.201627860030
correlation between body mass index and faecal microbiota from children.childhood obesity is an increasing problem at the global level and considered as a risk factor for obesity development and the associated co-morbidities in adult life. in this study, the occurrence of bacteroides fragilis group, clostridium spp., bifidobacterium spp. and escherichia coli in 84 faecal samples from 30 obese, 24 overweight and 30 lean children was verified by culture technique and quantitative determination by quantitative pcr. in addition, lactobacillus spp. and methanobrevibacter ...201626551842
deputizing services. 20164714493
failure of antibody formation after massive exposure to the rh (d) antigen. 20164977102
role of the fire service in road-traffic accidents. 20164845316
history of the wisconsin maternal mortality study and survey committee. 20164905769
electron microscopic study of meiosis in diploid male silkworms. 20164778507
regulation of triglyceride biosynthesis in adipose and intestinal tissue.the synthesis of phosphatidic acid and di- and triglycerides via the glycerol-3-phosphate pathway is markedly inhibited by 2-monooleyl ether in microsomal and whole cell preparations obtained from adipose and intestinal tissue. monoglycerides are also inhibitors under conditions in which their hydrolysis is minimized. a correlation between inhibition by, and the hydrolysis of, monoglycerides has been demonstrated. 2-monooleyl ether is the most effective inhibitor of the several mono- and di- eth ...20164715324
[autoimmune hepatitis]. 20164865241
assessment of behavior characteristics in mental retardates. 20164867070
[laryngo-tracheal lesions produced by endothracheal intubation in children. anatomical study of 53 cases]. 20164822603
systolic time intervals in valvular aortic stenosis and idiopathic hypertrophic subaortic stenosis. 20164734998
editorial: the significance of food poisoning in canada. 20164841214
inflammatory bowel disease in ankylosing spondylitis. 20165030136
[traffic facial injuries]. 20164884373
[trial in abdominal surgery of a new molecule, trimebutine, in resumption of postoperative transit]. 20165088602
[pathogenesis and prevention of diabetes mellitus and its complications]. 20165069982
motor proficiency and other variables in educable mentally retarded children. 20164898365
[detection of cellular radiation autoallergy in mice using a method of splenocyte migration inhibition]. 20164821651
sex roles in games. 20164766351
[term cervical pregnancy (author's transl)]. 20164747158
cargo cultism: a psychosocial study of melanesian millenarianism. 20164746308
[clinical use of prostaglandins type e(pge) and type a(pga)]. 20164939298
experimental blackout and the visual system. 20164860699
preliminary survey of medical care in hawaii. 1. hospital care. 20164876386
[hpl serum levels in intrauterine retardation of fetal growth during late pregnancy (author's transl)]. 20164848903
an indexing coverage study of toxicological literature. 20164715287
technical aspects of dr. kryter's paper "impairment to hearing from exposure to noise" with respect to the niosh statistics. 20164712552
[stereochemistry of ephedrine-like compounds. absolute configuration of the four isomeric cis- and trans-6-amino-6,7,8,9-tetrahydro-5h-benzocycloheptenols-(5)]. 20165053529
steroid therapy for tracheal stenosis in children: clinical experience in 4 children with severe strictures. 20164813126
self-evaluation by first-year medical students in a clinical science programme. 20164771337
hepatic protein and nucleic acid content in dipsosurus dorsalis following hypophysectomy and treatment with estradiol-17 and growth hormone. 20165029486
[graphic models of the flow of information in the health care of prague university students]. 20165045146
the du suppression test using 125 i-udr to define biochemical megaloblastosis. 20164716055
wegener's granulomatosis causing deafness. 20154704648
crushing chest injuries. 20154702862
crushing chest injuries. 20154702862
adult cardiovascular training programs. 20154606177
manipulation of the immune response. 20154593206
[gastroscopic diagnosis of generalized amyloidosis]. 20154544440
unhappiness and mental illness. 20154525690
application of stereotaxic methods in neuroradiologic diagnosis. 20154573723
the sleep habits and lifestyle of cigarette smokers. 20154456152
studies of red-cell membrane function in heterozygous beta thalassaemia and other hypochromic anaemias. 20154441462
[the epithelium formation capacity of chorioallantoic membrane. i. structural changes in ectodermal epithelium grafted with fixed and living tissues]. 20154458706
[the occurrence of milk protein allergy in the netherlands]. 20154640988
[anatomo-clinical and experimental study of paraquat poisoning. ultrastructural lesions]. 20154355682
[statistical characteristics of signal transmission in the nervous system]. 20154320075
[polyclinic academy of medicine]. 20154483641
career-ladder breakthrough. now, an associate degree without attending college. 20154485178
in memoriam p.e. lukomsky 1900-1974. 20154615878
the diagnosis and prevention of post operative venous thrombosis. 20154617329
proceedings: cellular kinetics in acute leukemia. 20154550057
[competition of renal effects of angiotensin ii and extracellular fluid volume receptors]. 20154330959
[metal chelates of a bispyridyl-tetrahydrothiazine dioxide]. 20154663589
[effects of dichlorvos and malathion on ascorbic acid level and the nutritional value of some vegetables]. 20154709861
[equipment and methods used in the hematologic services in czechoslovakia]. 20154653149
coronary arteriography: a review (second part). 20154614822
radiological case of the month. 20154854015
[hand burns caused by a laundry press]. 20154552000
effect of testosterone on the lh and fsh release induced by lh-releasing factor (lrf) in normal men. 20154581339
significance and comparison of two methods for platelet aggregation. 20154642544
[a leading soviet surgeon alekseĭ petrovich krymov (on the 100th anniversary of his birth)]. 20154568208
[diabetic encephalopathy]. 20154555153
the use of the xenopus oocyte to prove the existence of messenger sequences in high molecular weight rna. 20154512958
continuous elevation by spica cast in treatment of reflex sympathetic dystrophy. 20154452701
[electron microscopic study of the reversibility of sclerotic changes in the liver]. 20154615677
[the urinary excretion of lotrimazole in various age groups of children]. 20154762903
[simultaneous acute arterial thromboembolism in the four limbs]. 20154425117
[presidential address. health is harmony]. 20154337670
[irreversible lesions of the trigeminal nerve caused by chlorinated acetylenes in two patients]. 20154314203
k.r. eissler's discourse on hamlet. a symposium. hamlet and ressentiment. 20154578625
custody of children and the law. 20154765874
[ovarian vein syndrome]. 20154704157
[ovarian vein syndrome]. 20154704157
fifty years of milford hospital. 20154557834
visual effects of subfusional rates of high-intensity intermittent photic stimulation. 20154517391
[x-ray radioisotope diagnosis of tumor metastases of urogenital organs into the skeleton]. 20155063119
dialysis of poisons and drugs - annual review. 20154610970
[apparatus for automatic reinfusion of blood]. 20154720164
three hippies--a study of a late-adolescent identity formation. 20154476728
structure and sequence analyses of bacteroides proteins bvu_4064 and bf1687 reveal presence of two novel predominantly-beta domains, predicted to be involved in lipid and cell surface interactions.n-terminal domains of bvu_4064 and bf1687 proteins from bacteroides vulgatus and bacteroides fragilis respectively are members of the pfam family pf12985 (duf3869). proteins containing a domain from this family can be found in most bacteroides species and, in large numbers, in all human gut microbiome samples. both bvu_4064 and bf1687 proteins have a consensus lipobox motif implying they are anchored to the membrane, but their functions are otherwise unknown. the c-terminal half of bvu_4064 is a ...201525592227
sialic acid catabolism drives intestinal inflammation and microbial dysbiosis in mice.rapid shifts in microbial composition frequently occur during intestinal inflammation, but the mechanisms underlying such changes remain elusive. here we demonstrate that an increased caecal sialidase activity is critical in conferring a growth advantage for some bacteria including escherichia coli (e. coli) during intestinal inflammation in mice. this sialidase activity originates among others from bacteroides vulgatus, whose intestinal levels expand after dextran sulphate sodium administration ...201526303108
functional metagenomic discovery of bacterial effectors in the human microbiome and isolation of commendamide, a gpcr g2a/132 agonist.the trillions of bacteria that make up the human microbiome are believed to encode functions that are important to human health; however, little is known about the specific effectors that commensal bacteria use to interact with the human host. functional metagenomics provides a systematic means of surveying commensal dna for genes that encode effector functions. here, we examine 3,000 mb of metagenomic dna cloned from three phenotypically distinct patients for effectors that activate nf-κb, a tr ...201526283367
crohn associated microbial communities associated to colonic mucosal biopsies in patients of the western mediterranean.next generation sequencing approaches allow the retrieval of several orders of magnitude larger numbers of amplified single sequences in 16s rrna diversity surveys than classical methods. however, the sequences are only partial and thus lack sufficient resolution for a reliable identification. the opu approach used here, based on a tandem combination of high quality 454 sequences (mean >500 nuc) applying strict otu thresholds, and phylogenetic inference based on parsimony additions to preexistin ...201526275394
production of corticotropin-releasing factor and urocortin from human monocyte-derived dendritic cells is stimulated by commensal bacteria in intestine.to examine whether commensal bacteria are a contributing cause of stress-related mucosal inflammation.201425339828
antibacterial epipolythiodioxopiperazine and unprecedented sesquiterpene from pseudallescheria boydii, a beetle (coleoptera)-associated fungus.pseudallescheria boydii residing in the gut of coleopteran (holotrichia parallela) larva produces four new epipolythiodioxopiperazine (etp) boydines a-d (3-6) and two novel sesquiterpene boydenes a (7) and b (10), in addition to bisdethiobis(methylthio)-deacetylaranotin (1), bisdethiodi(methylthio)-deacetylapoaranotin (2), am6898 a (8) and ovalicin (9). the structure elucidation was accomplished by a combination of spectral methods with quantum chemical calculations of optical rotations and elec ...201425319640
model-based hypothesis of gut microbe populations and gut/brain barrier permeabilities in the development of regressive autism.regressive autism is a devastating disorder affecting children between the ages of 15-30 months. the disorder is characterized by the loss of social interaction and communication ability following otherwise healthy development. in spite of rising autism prevalence, current detection methods and treatment options for this disease are lacking. therefore, this study introduces a systems-level model, which suggests that gut microbes and intestinal inflammation influence the onset of regressive autis ...201425288537
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