Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| 2017 european guideline for the management of chancroid. | chancroid is a sexually acquired infection caused by haemophilus ducreyi. the infection is characterized by one or more genital ulcers, which are soft and painful, and regional lymphadenitis, which may develop into buboes. the infection may easily be misidentified due to its rare occurrence in europe and difficulties in detecting the causative pathogen. h. ducreyi is difficult to culture. nucleic acid amplification tests can demonstrate the bacterium in suspected cases. antibiotics are usually e ... | 2017 | 28081686 |
| a novel intrinsically disordered outer membrane lipoprotein of aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8. | intrinsically disordered proteins (idps) do not have a well-defined and stable 3-dimensional fold. some idps can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. a novel outer membrane lipoprotein, bacterial interleukin receptor i (bilri) of the opportunistic oral pathogen aggregatibacter actinomycetemcomitans binds a key gatekeeper proinflammatory cytokine interleukin (il)-1β. because the amino aci ... | 2017 | 27459270 |
| haemophilus ducreyi in asymptomatic prostitutes. | 2017 | 28223612 | |
| haemophilus ducreyi in asympmatic prostitutes. | 2017 | 28223611 | |
| haemophilus ducreyi seeks alternative carbon sources and adapts to nutrient stress and anaerobiosis during experimental infection of human volunteers. | haemophilus ducreyi causes the sexually transmitted disease chancroid in adults and cutaneous ulcers in children. in humans, h. ducreyi resides in an abscess and must adapt to a variety of stresses. previous studies (d. gangaiah, m. labandeira-rey, x. zhang, k. r. fortney, s. ellinger, b. zwickl, b. baker, y. liu, d. m. janowicz, b. p. katz, c. a. brautigam, r. s. munson, jr., e. j. hansen, and s. m. spinola, mbio 5:e01081-13, 2014, http://dx.doi.org/10.1128/mbio.01081-13) suggested that h. ducr ... | 2016 | 26930707 |
| correlates of bacterial ulcers and acute hsv-2 infection among men with genital ulcer disease in south africa: age, recent sexual behaviors, and hiv. | data from baseline surveys and sti/hiv laboratory tests (n=615 men) were used to examine correlates of bacterial ulcers (treponema pallidum, haemophilus ducreyi, or chlamydia trachomatis l1-l3 detected in ulcer) and acute hsv-2 ulcers (hsv-2 positive ulcer specimen, hsv-2 sero-negative, and negative for bacterial pathogens) vs. recurrent hsv-2 ulcers (sero-positive), separately. compared to men with recurrent hsv-2 ulcers, men with bacterial ulcers had larger ulcers but were less likely to be hi ... | 2016 | 28217702 |
| etiology of genital ulcer disease in male patients attending a sexually transmitted diseases clinic: first assessment in cuba. | sexually transmitted diseases (stds) and in particular genital ulcer disease (gud) have a major impact on morbidity and mortality in developing countries. the world health organization recommends the use of syndromic guidelines for the treatment of sexually transmitted infections (stis) in resource-constrained countries. surveillance of autochthonous etiologies provides epidemiological information contributing to the prevention and treatment of stis. we investigated the etiology and factors asso ... | 2016 | 27419816 |
| multiplex pcr testing for nine different sexually transmitted infections. | current sexually transmitted infection (sti) testing is not optimal due to delays in reporting or missed diagnoses due to a lack of comprehensive testing. the filmarray® (biofire diagnostics, llc, salt lake city, utah) is a user-friendly, fully automated, multiplex pcr system that is being developed for rapid point-of-care use. a research-use-only sti panel including multiple pcr primer sets for each organism was designed to detect chlamydia trachomatis, neisseria gonorrhoeae, treponema pallidum ... | 2016 | 26538551 |
| sexually transmitted diseases: epidemiological and clinical aspects in adults. | sexually transmitted diseases (stds) are the first 10 causes of unpleased diseases in young adult women in the world. the concept of stds includes a series of syndromes caused by pathogens that can be acquired by sexual intercourse or sexual activity.adolescents and young adults are responsible for only 25% of the sexually active population and they represent almost 50% of all newly acquired stds.in this way, we evaluated the epidemiological and clinical aspects of most relevant pathogens as nei ... | 2016 | 25532465 |
| impact of cdt toxin on human diseases. | cytolethal distending toxin (cdt) is found in gram-negative bacteria, especially in certain proteobacteria such as the pasteurellaceae family, including haemophilus ducreyi and aggregatibacter (actinobacillus) actinomycetemcomitans, in the enterobacteriaceae family and the campylobacterales order, including the campylobacter and helicobacter species. in vitro and in vivo studies have clearly shown that this toxin has a strong effect on cellular physiology (inflammation, immune response modulatio ... | 2016 | 27429000 |
| complete genome sequences of 11 haemophilus ducreyi isolates from children with cutaneous lesions in vanuatu and ghana. | haemophilus ducreyi causes chancroid and has recently been shown to be a significant cause of cutaneous lesions in tropical or subtropical regions where yaws is endemic. here, we report the draft genome assemblies for 11 cutaneous strains of haemophilus ducreyi, isolated from children in vanuatu and ghana. | 2016 | 27389258 |
| host polymorphisms in tlr9 and il10 are associated with the outcomes of experimental haemophilus ducreyi infection in human volunteers. | in humans inoculated with haemophilus ducreyi, there are host effects on the possible clinical outcomes-pustule formation versus spontaneous resolution of infection. however, the immunogenetic factors that influence these outcomes are unknown. here we examined the role of 14 single-nucleotide polymorphisms (snps) in 7 selected pathogen-recognition pathways and cytokine genes on the gradated outcomes of experimental infection. | 2016 | 27122592 |
| draft whole-genome sequence of haemophilus ducreyi strain auspng1, isolated from a cutaneous ulcer of a child from papua new guinea. | haemophilus ducreyi has recently emerged as a leading cause of cutaneous ulcers in the yaws-endemic areas of papua new guinea and other south pacific islands. here, we report the draft genome sequence of the h. ducreyi strain auspng1, isolated from a cutaneous ulcer of a child from papua new guinea. | 2016 | 26847887 |
| immunization with the haemophilus ducreyi trimeric autotransporter adhesin dsra with alum, cpg or imiquimod generates a persistent humoral immune response that recognizes the bacterial surface. | the ducreyi serum resistance a (dsra) protein of haemophilus ducreyi belongs to a large family of multifunctional outer membrane proteins termed trimeric autotransporter adhesins responsible for resistance to the bactericidal activity of human complement (serum resistance), agglutination and adhesion. the ability of dsra to confer serum resistance and bind extracellular matrix proteins lies in its n-terminal passenger domain. we have previously reported that immunization with a recombinant form ... | 2016 | 26812077 |
| haemophilus ducreyi: from sexually transmitted infection to skin ulcer pathogen. | this article provides an overview of the biology, epidemiology, clinical features, diagnostic tests, and treatment of haemophilus ducreyi infection, with special reference to the decline of chancroid and the recent emergence of h. ducreyi as a pathogen responsible for chronic limb ulceration clinically similar to yaws. | 2016 | 26658654 |
| first case of chancroid in 14 years at the largest sti clinic in paris, france. | we report the first case of chancroid seen at our clinic in 14 years. it was diagnosed by nuclear acid amplification test in a male patient returning from madagascar. although the disease is considered on the verge of disappearance even in tropical countries, its real potential for reemergence - due to new strains of haemophilus ducreyi, underreporting and a lack of widespread use of molecular testing - could be underestimated. | 2016 | 26378189 |
| tropical leg ulcers in children: more than yaws. | the management of yaws has changed in recent years. mass treatment with oral azithromycin has replaced intramuscular benzathine benzylpenicillin. treponemal and non-treponemal serology (equivalent to tpha and rpr) point-of-care blood testing is now available. in addition, recent studies in yaws endemic regions have shown that a significant number of leg ulcers in children which are clinically suggestive of yaws are caused by haemophilus ducreyi. it is noteworthy that the world health organizatio ... | 2016 | 26289420 |
| haemophilus ducreyi cutaneous ulcer strains diverged from both class i and class ii genital ulcer strains: implications for epidemiological studies. | haemophilus ducreyi has emerged as a major cause of cutaneous ulcers (cu) in yaws-endemic regions of the tropics in the south pacific, south east asia and africa. h. ducreyi was once thought only to cause the genital ulcer (gu) disease chancroid; gu strains belong to 2 distinct classes, class i and class ii. using whole-genome sequencing of 4 cu strains from samoa, 1 from vanuatu and 1 from papua new guinea, we showed that cu strains diverged from the class i strain 35000hp and that one cu strai ... | 2016 | 28027326 |
| yaws: towards the who eradication target. | in 2012 who declared a target to eradicate yaws by 2020. the cornerstone of this strategy is community mass treatment with azithromycin. initial studies suggest this is a very effective tool that may be capable of interrupting transmission. alongside this there has been progress in the development and validation of diagnostic tests for yaws. several new challenges have also emerged, in particular, evidence that haemophilus ducreyi can cause phenotypically similar ulcers in yaws endemic communiti ... | 2016 | 27268712 |
| epidemiology of haemophilus ducreyi infections. | the global epidemiology of haemophilus ducreyi infections is poorly documented because of difficulties in confirming microbiological diagnoses. we evaluated published data on the proportion of genital and nongenital skin ulcers caused by h. ducreyi before and after introduction of syndromic management for genital ulcer disease (gud). before 2000, the proportion of gud caused by h. ducreyi ranged from 0.0% to 69.0% (35 studies in 25 countries). after 2000, the proportion ranged from 0.0% to 15.0% ... | 2016 | 26694983 |
| the human skin microbiome associates with the outcome of and is influenced by bacterial infection. | the influence of the skin microbiota on host susceptibility to infectious agents is largely unexplored. the skin harbors diverse bacterial species that may promote or antagonize the growth of an invading pathogen. we developed a human infection model for haemophilus ducreyi in which human volunteers are inoculated on the upper arm. after inoculation, papules form and either spontaneously resolve or progress to pustules. to examine the role of the skin microbiota in the outcome of h. ducreyi infe ... | 2015 | 26374122 |
| challenges and key research questions for yaws eradication. | yaws is endemic in west africa, southeast asia, and the pacific region. to eradicate yaws by 2020, who has launched a campaign of mass treatment with azithromycin. progress has been made towards achievement of this ambitious goal, including the validation of point-of-care and molecular diagnostic tests and piloting of the strategy in several countries, including ghana, vanuatu, and papua new guinea. gaps in knowledge need to be addressed to allow refinement of the eradication strategy. studies e ... | 2015 | 26362174 |
| haemophilus ducreyi cutaneous ulcer strains are nearly identical to class i genital ulcer strains. | although cutaneous ulcers (cu) in the tropics is frequently attributed to treponema pallidum subspecies pertenue, the causative agent of yaws, haemophilus ducreyi has emerged as a major cause of cu in yaws-endemic regions of the south pacific islands and africa. h. ducreyi is generally susceptible to macrolides, but cu strains persist after mass drug administration of azithromycin for yaws or trachoma. h. ducreyi also causes genital ulcers (gu) and was thought to be exclusively transmitted by mi ... | 2015 | 26147869 |
| dksa and (p)ppgpp have unique and overlapping contributions to haemophilus ducreyi pathogenesis in humans. | the (p)ppgpp-mediated stringent response is important for bacterial survival in nutrient limiting conditions. for maximal effect, (p)ppgpp interacts with the cofactor dksa, which stabilizes (p)ppgpp's interaction with rna polymerase. we previously demonstrated that (p)ppgpp was required for the virulence of haemophilus ducreyi in humans. here, we constructed an h. ducreyi dksa mutant and showed it was also partially attenuated for pustule formation in human volunteers. to understand the roles of ... | 2015 | 26056381 |
| molecular phylogenetic analysis of non-sexually transmitted strains of haemophilus ducreyi. | haemophilus ducreyi, the etiologic agent of chancroid, has been previously reported to show genetic variance in several key virulence factors, placing strains of the bacterium into two genetically distinct classes. recent studies done in yaws-endemic areas of the south pacific have shown that h. ducreyi is also a major cause of cutaneous limb ulcers (clu) that are not sexually transmitted. to genetically assess clu strains relative to the previously described class i, class ii phylogenetic hiera ... | 2015 | 25774793 |
| a cross-sectional study of 'yaws' in districts of ghana which have previously undertaken azithromycin mass drug administration for trachoma control. | yaws, caused by treponema pallidum ssp. pertenue, is reportedly endemic in ghana. mass distribution of azithromycin is now the cornerstone of the who yaws eradication campaign. mass distribution of azithromycin at a lower target dose was previously undertaken in two regions of ghana for the control of trachoma. ongoing reporting of yaws raises the possibility that resistance may have emerged in t. pallidum pertenue, or that alternative infections may be responsible for some of the reported cases ... | 2015 | 25632942 |
| defining potential vaccine targets of haemophilus ducreyi trimeric autotransporter adhesin dsra. | haemophilus ducreyi is the causative agent of the sexually transmitted genital ulcer disease chancroid. strains of h. ducreyi are grouped in two classes (i and ii) based on genotypic and phenotypic differences, including those found in dsra, an outer membrane protein belonging to the family of multifunctional trimeric autotransporter adhesins. dsra is a key serum resistance factor of h. ducreyi that prevents binding of natural igm at the bacterial surface and functions as an adhesin to fibronect ... | 2015 | 25897604 |
| development and validation of a high-throughput cell-based screen to identify activators of a bacterial two-component signal transduction system. | cpxra is a two-component signal transduction system (2csts) found in many drug-resistant gram-negative bacteria. in response to periplasmic stress, cpxa autophosphorylates and donates a phosphoryl group to its cognate response regulator, cpxr. phosphorylated cpxr (cpxr-p) upregulates genes involved in membrane repair and downregulates multiple genes that encode virulence factors, which are trafficked across the cell membrane. mutants that constitutively activate cpxra in salmonella enterica sero ... | 2015 | 25870061 |
| phosphoethanolamine transferase lpta in haemophilus ducreyi modifies lipid a and contributes to human defensin resistance in vitro. | haemophilus ducreyi resists the cytotoxic effects of human antimicrobial peptides (aps), including α-defensins, β-defensins, and the cathelicidin ll-37. resistance to ll-37, mediated by the sensitive to antimicrobial peptide (sap) transporter, is required for h. ducreyi virulence in humans. cationic aps are attracted to the negatively charged bacterial cell surface. in other gram-negative bacteria, modification of lipopolysaccharide or lipooligosaccharide (los) by the addition of positively char ... | 2015 | 25902140 |
| a semi-multifunctional sialyltransferase from bibersteinia trehalosi and its comparison to the pasteurella multocida st1 mutants. | sialic acids are well known for their crucial roles in many physiological and pathological processes. improvement in the efficacy of protein drugs, an increase in the anti-inflammatory activity of intravenous immunoglobulin, preparation of infant milk and the diagnosis of diseases are examples of why there is a need for efficient in vitro sialylation. sialyltransferases are crucial enzymes for the synthesis of sialo-oligosaccharides. here, we introduce a new α2,3-sialyltransferase from bacteria ... | 2015 | 26477829 |
| antimicrobial activity of plant extracts against sexually transmitted pathogens. | comprehensive management of sexually transmitted infections (stis) using vaginal or rectal microbicide-based intervention is one of the strategies for prevention of hiv infection. herbal products have been used for treating stis traditionally. herein, we present in vitro activity of 10 plant extracts and their 34 fractions against three sexually transmitted/reproductive tract pathogens - neisseria gonorrhoeae, haemophilus ducreyi and candida albicans. the plant parts were selected; the extracts/ ... | 2015 | 25427632 |
| distinct roles for cdta and cdtc during intoxication by cytolethal distending toxins. | cytolethal distending toxins (cdts) are heterotrimeric protein exotoxins produced by a diverse array of gram-negative pathogens. the enzymatic subunit, cdtb, possesses dnase and phosphatidylinositol 3-4-5 trisphosphate phosphatase activities that induce host cell cycle arrest, cellular distension and apoptosis. to exert cyclomodulatory and cytotoxic effects cdts must be taken up from the host cell surface and transported intracellularly in a manner that ultimately results in localization of cdtb ... | 2015 | 26618479 |
| structure of bama, an essential factor in outer membrane protein biogenesis. | outer membrane protein (omp) biogenesis is an essential process for maintaining the bacterial cell envelope and involves the β-barrel assembly machinery (bam) for omp recognition, folding and assembly. in escherichia coli this function is orchestrated by five proteins: the integral outer membrane protein bama of the omp85 superfamily and four associated lipoproteins. to unravel the mechanism underlying omp folding and insertion, the structure of the e. coli bama β-barrel and p5 domain was determ ... | 2014 | 24914988 |
| assessing the antibiotic potential of essential oils against haemophilus ducreyi. | haemophilus ducreyi is the bacterium responsible for the genital ulcer disease chancroid, a cofactor for the transmission of hiv, and it is resistant to many antibiotics. with the goal of exploring possible alternative treatments, we tested essential oils (eos) for their efficacy as antimicrobial agents against h. ducreyi. | 2014 | 24885682 |
| haemophilus ducreyi associated with skin ulcers among children, solomon islands. | during a survey of yaws prevalence in the solomon islands, we collected samples from skin ulcers of 41 children. using pcr, we identified haemophilus ducreyi infection in 13 (32%) children. pcr-positive and pcr-negative ulcers were phenotypically indistinguishable. emergence of h. ducreyi as a cause of nongenital ulcers may affect the world health organization's yaws eradication program. | 2014 | 25271477 |
| a (p)ppgpp-null mutant of haemophilus ducreyi is partially attenuated in humans due to multiple conflicting phenotypes. | (p)ppgpp responds to nutrient limitation through a global change in gene regulation patterns to increase survival. the stringent response has been implicated in the virulence of several pathogenic bacterial species. haemophilus ducreyi, the causative agent of chancroid, has homologs of both rela and spot, which primarily synthesize and hydrolyze (p)ppgpp in escherichia coli. we constructed rela and rela spot deletion mutants to assess the contribution of (p)ppgpp to h. ducreyi pathogenesis. both ... | 2014 | 24914217 |
| outer membrane protein p4 is not required for virulence in the human challenge model of haemophilus ducreyi infection. | bacterial lipoproteins often play important roles in pathogenesis and can stimulate protective immune responses. such lipoproteins are viable vaccine candidates. haemophilus ducreyi, which causes the sexually transmitted disease chancroid, expresses a number of lipoproteins during human infection. one such lipoprotein, ompp4, is homologous to the outer membrane lipoprotein e (p4) of h. influenzae. in h. influenzae, e (p4) stimulates production of bactericidal and protective antibodies and contri ... | 2014 | 24961160 |
| cytolethal distending toxins require components of the er-associated degradation pathway for host cell entry. | intracellular acting protein exotoxins produced by bacteria and plants are important molecular determinants that drive numerous human diseases. a subset of these toxins, the cytolethal distending toxins (cdts), are encoded by several gram-negative pathogens and have been proposed to enhance virulence by allowing evasion of the immune system. cdts are trafficked in a retrograde manner from the cell surface through the golgi apparatus and into the endoplasmic reticulum (er) before ultimately reach ... | 2014 | 25078082 |
| inflammatory and bone remodeling responses to the cytolethal distending toxins. | the cytolethal distending toxins (cdts) are a family of exotoxins produced by a wide range of gram-negative bacteria. they are known for causing genotoxic stress to the cell, resulting in growth arrest and eventually apoptotic cell death. nevertheless, there is evidence that cdts can also perturb the innate immune responses, by regulating inflammatory cytokine production and molecular mediators of bone remodeling in various cell types. these cellular and molecular events may in turn have an effe ... | 2014 | 24709959 |
| cytolethal distending toxin b as a cell-killing component of tumor-targeted anthrax toxin fusion proteins. | cytolethal distending toxin (cdt) is produced by gram-negative bacteria of several species. it is composed of three subunits, cdta, cdtb, and cdtc, with cdtb being the catalytic subunit. we fused cdtb from haemophilus ducreyi to the n-terminal 255 amino acids of bacillus anthracis toxin lethal factor (lfn) to design a novel, potentially potent antitumor drug. as a result of this fusion, cdtb was transported into the cytosol of targeted cells via the efficient delivery mechanism of anthrax toxin. ... | 2014 | 24434511 |
| [cytolethal distending toxins]. | cytolethal distending toxins (cdt) are intracellularly acting proteins which interfere with the eukaryotic cell cycle. they are produced by gram-negative bacteria with affinity to mucocutaneous surfaces and could play a role in the pathogenesis of various mammalian diseases. the functional toxin is composed of three proteins: cdtb entering the nucleus and by its nuclease activity inducing nuclear fragmentation and chromatin disintegration, cdta, and cdtc, the two latter being responsible for tox ... | 2014 | 25025680 |
| virtual screening of phytochemicals to novel targets in haemophilus ducreyi towards the treatment of chancroid. | conventionally, drugs are discovered by testing chemically synthesized compounds against a battery of in vivo biological screens. information technology and omic science enabled us for high throughput screening of compound libraries against biological targets and hits are then tested for efficacy in cells or animals. chancroid, caused by haemophilus ducreyi is a public health problem and has been recognized as a cofactor for human immunodeficiency virus (hiv) transmission. it facilitates hiv tra ... | 2014 | 25258485 |
| haemophilus ducreyi rpoe and cpxra appear to play distinct yet complementary roles in regulation of envelope-related functions. | haemophilus ducreyi causes the sexually transmitted disease chancroid and a chronic limb ulceration syndrome in children. in humans, h. ducreyi is found in an abscess and overcomes a hostile environment to establish infection. to sense and respond to membrane stress, bacteria utilize two-component systems (tcss) and extracytoplasmic function (ecf) sigma factors. we previously showed that activation of cpxra, the only intact tcs in h. ducreyi, does not regulate homologues of envelope protein fold ... | 2014 | 25201944 |
| haemophilus ducreyi as a cause of skin ulcers. | 2014 | 25103386 | |
| haemophilus ducreyi as a cause of skin ulcers in children from a yaws-endemic area of papua new guinea: a prospective cohort study. | skin infections with ulceration are a major health problem in countries of the south pacific region. yaws, caused by treponema pallidum subspecies pertenue and diagnosed by the presence of skin ulcers and a reactive syphilis serology, is one major cause, but this infection can be confused clinically with ulcers due to other causative agents. we investigated t pallidum pertenue and another bacterium known to cause skin infections in the pacific islands-haemophilus ducreyi-as causes of skin ulcera ... | 2014 | 25103064 |
| haemophilus ducreyi: a newly recognised cause of chronic skin ulceration. | 2014 | 25103048 | |
| a multicenter prospective trial to asses a new real-time polymerase chain reaction for detection of treponema pallidum, herpes simplex-1/2 and haemophilus ducreyi in genital, anal and oropharyngeal ulcers. | treponema pallidum, herpes simplex virus types 1 or 2 (hsv-1/2) and haemophilus ducreyi are sexually transmitted pathogens that can cause genital, anal and oropharyngeal ulcers. laboratory evaluation of these pathogens in ulcers requires different types of specimens and tests, increasing the risk of improper specimen handling and time lapse until analysis. we sought to develop a new real-time pcr (tp-hd-hsv1/2 pcr) to facilitate the detection of t. pallidum, hsv-1/2 and h. ducreyi in ulcers. the ... | 2014 | 24909546 |
| the haemophilus ducreyi lspa1 protein inhibits phagocytosis by using a new mechanism involving activation of c-terminal src kinase. | haemophilus ducreyi causes chancroid, a sexually transmitted infection. a primary means by which this pathogen causes disease involves eluding phagocytosis; however, the molecular basis for this escape mechanism has been poorly understood. here, we report that the lspa virulence factors of h. ducreyi inhibit phagocytosis by stimulating the catalytic activity of c-terminal src kinase (csk), which itself inhibits src family protein tyrosine kinases (sfks) that promote phagocytosis. inhibitory acti ... | 2014 | 24902122 |
| the haemophilus ducreyi trimeric autotransporter adhesin dsra protects against an experimental infection in the swine model of chancroid. | adherence of pathogens to cellular targets is required to initiate most infections. defining strategies that interfere with adhesion is therefore important for the development of preventative measures against infectious diseases. as an adhesin to host extracellular matrix proteins and human keratinocytes, the trimeric autotransporter adhesin dsra, a proven virulence factor of the gram-negative bacterium haemophilus ducreyi, is a potential target for vaccine development. a recombinant form of the ... | 2014 | 24844153 |
| epidemiology, clinical features, diagnosis and treatment of haemophilus ducreyi - a disappearing pathogen? | chancroid, caused by haemophilus ducreyi, has declined in importance as a sexually transmitted pathogen in most countries where it was previously endemic. the global prevalence of chancroid is unknown as most countries lack the required laboratory diagnostic capacity and surveillance systems to determine this. h. ducreyi has recently emerged as a cause of chronic skin ulceration in some south pacific islands. although no antimicrobial susceptibility data for h. ducreyi have been published for tw ... | 2014 | 24597521 |
| chancroid - desperate patient makes own diagnosis. | we report a case of chancroid in a white heterosexual man in the united kingdom. this patient was seen by four separate health services over a period of five weeks with excruciatingly painful penile ulcers. despite several negative herpes simplex virus polymerase chain reaction tests and a self-diagnosis of chancroid, he was repeatedly offered multiple courses of aciclovir. this case highlights the need for awareness of alternative diagnoses in persistent cases of genital ulcer disease. | 2014 | 24535694 |
| haemophilus ducreyi hfq contributes to virulence gene regulation as cells enter stationary phase. | to adapt to stresses encountered in stationary phase, gram-negative bacteria utilize the alternative sigma factor rpos. however, some species lack rpos; thus, it is unclear how stationary-phase adaptation is regulated in these organisms. here we defined the growth-phase-dependent transcriptomes of haemophilus ducreyi, which lacks an rpos homolog. compared to mid-log-phase organisms, cells harvested from the stationary phase upregulated genes encoding several virulence determinants and a homolog ... | 2014 | 24520065 |
| carbon storage regulator a contributes to the virulence of haemophilus ducreyi in humans by multiple mechanisms. | the carbon storage regulator a (csra) controls a wide variety of bacterial processes, including metabolism, adherence, stress responses, and virulence. haemophilus ducreyi, the causative agent of chancroid, harbors a homolog of csra. here, we generated an unmarked, in-frame deletion mutant of csra to assess its contribution to h. ducreyi pathogenesis. in human inoculation experiments, the csra mutant was partially attenuated for pustule formation compared to its parent. deletion of csra resulted ... | 2013 | 23230298 |
| cellular interactions of the cytolethal distending toxins from escherichia coli and haemophilus ducreyi. | the cytolethal distending toxins (cdts) compose a subclass of intracellularly acting genotoxins produced by many gram-negative pathogenic bacteria that disrupt the normal progression of the eukaryotic cell cycle. here, the intoxication mechanisms of cdts from escherichia coli (ec-cdt) and haemophilus ducreyi (hd-cdt), which share limited amino acid sequence homology, were directly compared. ec-cdt and hd-cdt shared comparable in vitro dnase activities of the cdtb subunits, saturable cell surface ... | 2013 | 23306199 |
| professor cathy ison: her contribution to the science and practice of sti control. | 2013 | 24243713 | |
| etiology of genital ulcer disease and association with hiv infection in malawi. | the world health organization recommends the use of syndromic management for patients presenting with genital ulcer disease (gud) in developing countries. however, effective treatment guidelines depend on a current country-specific gud etiological profile, which may change over time. | 2013 | 24220352 |
| trimeric autotransporter dsra is a major mediator of fibrinogen binding in haemophilus ducreyi. | haemophilus ducreyi is the etiologic agent of the sexually transmitted genital ulcer disease chancroid. in both natural and experimental chancroid, h. ducreyi colocalizes with fibrin at the base of the ulcer. fibrin is obtained by cleavage of the serum glycoprotein fibrinogen (fg) by thrombin to initiate formation of the blood clot. fg binding proteins are critical virulence factors in medically important gram-positive bacteria. h. ducreyi has previously been shown to bind fg in an agglutination ... | 2013 | 24042118 |
| the haemophilus ducreyi fis protein is involved in controlling expression of the lspb-lspa2 operon and other virulence factors. | expression of the lspb-lspa2 operon encoding a virulence-related two-partner secretion system in haemophilus ducreyi 35000hp is directly regulated by the cpxra regulatory system (m. labandeira-rey, j. r. mock, and e. j. hansen, infect. immun. 77:3402-3411, 2009). in the present study, we show that this secretion system is also regulated by the small nucleoid-associated protein fis. inactivation of the h. ducreyi fis gene resulted in a reduction in expression of both the h. ducreyi lspb and lspa2 ... | 2013 | 23980107 |
| haemophilus ducreyi infection induces activation of the nlrp3 inflammasome in nonpolarized but not in polarized human macrophages. | recognition of microbial infection by certain intracellular pattern recognition receptors leads to the formation of a multiprotein complex termed the inflammasome. inflammasome assembly activates caspase-1 and leads to cleavage and secretion of the proinflammatory cytokines interleukin-1 beta (il-1β) and il-18, which help control many bacterial pathogens. however, excessive inflammation mediated by inflammasome activation can also contribute to immunopathology. here, we investigated whether haem ... | 2013 | 23753629 |
| activation of cpxra in haemophilus ducreyi primarily inhibits the expression of its targets, including major virulence determinants. | haemophilus ducreyi causes chancroid, a genital ulcer disease that facilitates the transmission of human immunodeficiency virus type 1. in humans, h. ducreyi is surrounded by phagocytes and must adapt to a hostile environment to survive. to sense and respond to environmental cues, bacteria frequently use two-component signal transduction (2cst) systems. the only obvious 2cst system in h. ducreyi is cpxra; cpxr is a response regulator, and cpxa is a sensor kinase. previous studies by hansen and c ... | 2013 | 23729647 |
| resveratrol is cidal to both classes of haemophilus ducreyi. | resveratrol, a polyphenolic phytoalexin, is produced by plants in response to infection and has antibacterial activity. haemophilus ducreyi is a gram-negative bacterium that is the causative agent of the sexually transmitted disease chancroid. this study employed minimum cidal concentration (mcc) assays to evaluate the potential of resveratrol as a microbicide against h. ducreyi. five class i and four class ii strains of h. ducreyi tested had mccs ≤500 μg/ml. resveratrol was also tested against ... | 2013 | 23541304 |
| mutational analysis of hemoglobin binding and heme utilization by a bacterial hemoglobin receptor. | iron is an essential nutrient for most living organisms. to acquire iron from their environment, gram-negative bacteria use tonb-dependent transporters that bind host proteins at the bacterial surface and transport iron or heme to the periplasm via the ton machinery. tonb-dependent transporters are barrel-shaped outer membrane proteins with 22 transmembrane domains, 11 surface-exposed loops, and a plug domain that occludes the pore. to identify key residues of tonb-dependent transporters involve ... | 2013 | 23667232 |
| etiology of genital ulcer disease in a sexually transmitted infection reference center in manaus, brazilian amazon. | to determine the etiology and factors associated with genital ulcer disease (gud) among patients presenting to a sexually transmitted infections clinic in manaus, brazil; and to compare a multiplex polymerase chain reaction (m-pcr) assay for the diagnosis of gud with standard methods. | 2013 | 23704961 |
| structural insight into the biogenesis of β-barrel membrane proteins. | β-barrel membrane proteins are essential for nutrient import, signalling, motility and survival. in gram-negative bacteria, the β-barrel assembly machinery (bam) complex is responsible for the biogenesis of β-barrel membrane proteins, with homologous complexes found in mitochondria and chloroplasts. here we describe the structure of bama, the central and essential component of the bam complex, from two species of bacteria: neisseria gonorrhoeae and haemophilus ducreyi. bama consists of a large p ... | 2013 | 23995689 |
| permeases of the sap transporter are required for cathelicidin resistance and virulence of haemophilus ducreyi in humans. | haemophilus ducreyi encounters several classes of antimicrobial peptides (aps) in vivo and utilizes the sensitive-to-antimicrobial-peptides (sap) transporter as one mechanism of ap resistance. a mutant lacking the periplasmic solute-binding component, sapa, was somewhat more sensitive to the cathelicidin ll-37 than the parent strain and was partially attenuated for virulence. the partial attenuation led us to question whether the transporter is fully abrogated in the sapa mutant. | 2012 | 22930807 |
| prevalence and associations of genital ulcer and urethral pathogens in men presenting with genital ulcer syndrome to primary health care clinics in south africa. | this study aimed to determine the prevalence of genital ulcer and urethral pathogens, as well as their association with clinical features, in men with genital ulcer disease (gud) enrolled in a clinical trial. | 2012 | 23064538 |
| etiologic pattern of genital ulcers in lusaka, zambia: has chancroid been eliminated? | genital ulcers are a public health problem in developing countries. the world health organization recommends the use of syndromic guidelines for sexually transmitted infection treatment in resource-constrained countries. monitoring local etiologies provides information that may aid policy for sexually transmitted infection treatment. we investigated the etiology of genital ulcer disease among outpatients in lusaka, zambia. | 2012 | 23001266 |
| genital ulcer disease in india: etiologies and performance of current syndrome guidelines. | in india, genital ulcer disease (gud) syndrome is clinically classified as herpetic or nonherpetic and managed accordingly; laboratory support is unavailable at most health facilities. we undertook a study to determine the etiology of guds in men presenting to sexually transmitted infection (sti) clinics and assess the performance of the national algorithm for syndromic management of herpetic and nonherpetic guds in india. | 2012 | 23064541 |
| haemophilus ducreyi-induced interleukin-10 promotes a mixed m1 and m2 activation program in human macrophages. | during microbial infection, macrophages are polarized to classically activated (m1) or alternatively activated (m2) cells in response to microbial components and host immune mediators. proper polarization of macrophages is critical for bacterial clearance. to study the role of macrophage polarization during haemophilus ducreyi infection, we analyzed a panel of macrophage surface markers in skin biopsy specimens of pustules obtained from experimentally infected volunteers. lesional macrophages ex ... | 2012 | 23027536 |
| protocol for the use of enzyme-linked hybridization assays for genital ulcer disease. | etiologic agents of genital ulcer disease include herpes simplex 1 and 2, treponema pallidum pallidum, haemophilus ducreyi, and klebsiella granulomatis. the advent of pcr has allowed for more rapid and sensitive detection of microbial pathogens. in this protocol, we describe the simultaneous detection of these five pathogens and an internal control using a single-tube multiplex pcr and colorimetric enzyme-linked amplicon hybridization assay. | 2012 | 22782821 |
| carbon monoxide binding to the heme group at the dimeric interface modulates structure and copper accessibility in the cu,zn superoxide dismutase from haemophilus ducreyi: in silico and in vitro evidences. | x-ray absorption near-edge structure (xanes) spectroscopy and molecular dynamics (md) simulations have been jointly applied to the study of the cu,zn superoxide dismutase from haemophilus ducreyi (hdsod) in interaction with the carbon monoxide molecule. the configurational flexibility of the fe(ii)-heme group, intercalated between the two subunits, has been sampled by md simulations and included in the xanes data analysis without optimization in the structural parameter space. our results provid ... | 2012 | 22686457 |
| haemophilus ducreyi and treponema pallidum co-infection in an hiv-negative male presenting with anal ulceration. | 2012 | 22340627 | |
| diagnosis and management of genital ulcers. | herpes simplex virus infection and syphilis are the most common causes of genital ulcers in the united states. other infectious causes include chancroid, lymphogranuloma venereum, granuloma inguinale (donovanosis), secondary bacterial infections, and fungi. noninfectious etiologies, including sexual trauma, psoriasis, behçet syndrome, and fixed drug eruptions, can also lead to genital ulcers. although initial treatment of genital ulcers is generally based on clinical presentation, the following ... | 2012 | 22335265 |
| periodic presumptive treatment of curable sexually transmitted infections among sex workers: recent experience with implementation. | curable sexually transmitted infections (stis) are common occupational hazards for female sex workers in low-income and middle-income countries. yet, most infections are asymptomatic and sensitive screening tests are rarely affordable or feasible. periodic presumptive treatment (ppt) has been used as a component of sti control interventions to rapidly reduce sti prevalence. | 2012 | 22156895 |
| On the possible roles of N-terminal His-rich domains of Cu,Zn SODs of some Gram-negative bacteria. | The Cu,Zn superoxide dismutases (Cu,Zn SOD) isolated from some Gram-negative bacteria possess a His-rich N-terminal metal binding extension. The N-terminal domain of Haemophilus ducreyi Cu,Zn SOD has been previously proposed to play a copper(II)-, and may be a zinc(II)-chaperoning role under metal ion starvation, and to behave as a temporary (low activity) superoxide dismutating center if copper(II) is available. The N-terminal extension of Cu,Zn SOD from Actinobacillus pleuropneumoniae starts w ... | 2012 | 22105012 |
| on the evolution of the sexually transmitted bacteria haemophilus ducreyi and klebsiella granulomatis. | haemophilus ducreyi and klebsiella (calymmatobacterium) granulomatis are sexually transmitted bacteria that cause characteristic, persisting ulceration on external genitals called chancroid and granuloma inguinale, respectively. those ulcers are endemic in developing countries or exist, as does granuloma inguinale, only in some geographic "hot spots."h. ducreyi is placed in the genus haemophilus (family pasteurellacae); however, this phylogenetic position is not obvious. the multiple ways in whi ... | 2011 | 22239475 |
| hiv and other sexually transmitted infections in a cohort of women involved in high-risk sexual behavior in kampala, uganda. | uganda has long been successful in controlling the hiv epidemic; however, there is evidence that hiv prevalence and incidence are increasing again. data on the hiv/sti epidemic among sex workers are so far lacking from uganda. this paper describes the baseline epidemiology of hiv/sti in a newly established cohort of women involved in high-risk sexual behavior in kampala, uganda. | 2011 | 23330152 |
| passive immunization with a polyclonal antiserum to the hemoglobin receptor of haemophilus ducreyi confers protection against a homologous challenge in the experimental swine model of chancroid. | haemophilus ducreyi, the etiologic agent of chancroid, has an obligate requirement for heme. heme is acquired by h. ducreyi from its human host using tonb-dependent transporters expressed at its bacterial surface. of 3 tonb-dependent transporters encoded in the genome of h. ducreyi, only the hemoglobin receptor, hgba, is required to establish infection during the early stages of the experimental human model of chancroid. active immunization with a native preparation of hgba (nhgba) confers compl ... | 2011 | 21646451 |
| oral immunization using hgba in a recombinant chancroid vaccine delivered by attenuated salmonella typhimurium sl3261 in the temperature-dependent rabbit model. | chancroid, a sexually transmitted genital ulcer disease caused by the gram-negative bacterium haemophilus ducreyi, facilitates the acquisition and transmission of hiv. an effective vaccine against chancroid has not been developed. in this preliminary study, the gene encoding the h. ducreyi outer membrane hemoglobin receptor hgba was cloned into the plasmid ptetnir15. the recombinant construct was introduced into the attenuated salmonella typhimurium sl3261 strain and stable expression was induce ... | 2011 | 22100216 |
| Expression of the Flp proteins by Haemophilus ducreyi is necessary for virulence in human volunteers. | Haemophilus ducreyi, the causative agent of the sexually transmitted disease chancroid, contains a flp (fimbria like protein) operon that encodes proteins predicted to contribute to adherence and pathogenesis. H. ducreyi mutants that lack expression of Flp1 and Flp2 or TadA, which has homology to NTPases of type IV secretion systems, have decreased abilities to attach to and form microcolonies on human foreskin fibroblasts (HFF). A tadA mutant is attenuated in its ability to cause disease in hum ... | 2011 | 21939541 |
| Electrotransformation of Haemophilus parasuis with in vitro modified DNA based on a novel shuttle vector. | The objective of the present study was to establish a valid transformation method of Haemophilus parasuis, the causative agent of Glässer's disease in pigs, using a novel H. parasuis-Escherichia coli shuttle vector. A 4.2kb endogenous plasmid pYC93 was extracted from an H. parasuis field isolate and completely sequenced. Analysis of pYC93 revealed a region approximately 800bp showing high homology with the defined replication origin oriV of pLS88, a native plasmid identified in Haemophilus ducre ... | 2011 | 21925811 |
| sialylation of lipooligosaccharides is dispensable for the virulence of haemophilus ducreyi in humans. | sialylated glycoconjugates on the surfaces of mammalian cells play important roles in intercellular communication and self-recognition. the sialic acid preferentially expressed in human tissues is n-acetylneuraminic acid (neu5ac). in a process called molecular mimicry, many bacterial pathogens decorate their cell surface glycolipids with neu5ac. incorporation of neu5ac into bacterial glycolipids promotes bacterial interactions with host cell receptors called siglecs. these interactions affect ba ... | 2011 | 22144477 |
| identification and characterization of a heme periplasmic-binding protein in haemophilus ducreyi. | haemophilus ducreyi, a gram-negative and heme-dependent bacterium, is the causative agent of chancroid, a genital ulcer sexually transmitted infection. heme acquisition in h. ducreyi proceeds via a receptor mediated process in which the initial event involves binding of hemoglobin and heme to their cognate outer membrane proteins, hgba and tdha, respectively. following this specific interaction, the fate of the periplasmic deposited heme is unclear. using protein expression profiling of the h. d ... | 2011 | 21347851 |
| deletion of mtrc in haemophilus ducreyi increases sensitivity to human antimicrobial peptides and activates the cpxra regulon. | haemophilus ducreyi resists killing by antimicrobial peptides encountered during human infection, including cathelicidin ll-37, a-defensins, and ß-defensins. in this study, we examined the role of the proton motive force-dependent multiple transferable resistance (mtr) transporter in antimicrobial peptide resistance in h. ducreyi. we found a proton motive force-dependent effect on h. ducreyi's resistance to ll-37 and ß-defensin hbd-3, but not a-defensin hnp-2. deletion of the membrane fusion pro ... | 2011 | 21444663 |
| genetics and molecular specificity of sialylation of histophilus somni lipooligosaccharide (los) and the effect of los sialylation on toll-like receptor-4 signaling. | histophilus somni is an etiologic agent of bovine respiratory and systemic diseases. most pathogenic strains of h. somni that have been tested (36 of 42) are able to utilize n-acetyl-5-neuraminic acid (neu5ac) to sialylate their lipooligosaccharide (los). homologs of all the genes required for transport, metabolism, and regulation of neu5ac in haemophilus influenzae were identified in the sequenced genomes of h. somni. three open reading frames (orfs) in h. somni strain 2336 were identified that ... | 2011 | 21482041 |
| rapid divergence of two classes of haemophilus ducreyi. | haemophilus ducreyi, the etiologic agent of chancroid, expresses variants of several key virulence factors. while previous reports suggested that h. ducreyi strains formed two clonal populations, the differences between, and diversity within, these populations were unclear. to assess their variability, we examined sequence diversity at 11 h. ducreyi loci, including virulence and housekeeping genes, augmenting published data sets with pcr-amplified genes to acquire data for at least 10 strains at ... | 2011 | 21515774 |
| european guideline for the management of chancroid, 2011. | chancroid is a sexually acquired disease caused by haemophilus ducreyi. the infection is characterized by one or more genital ulcers, which are soft and painful, and regional lymphadenitis which may develop into buboes. the infection may easily be misidentified due to its rare occurrence in europe and difficulties in detecting the causative pathogen. h. ducreyi is difficult to culture. polymerase chain reaction (pcr) can demonstrate the bacterium in suspected cases. antibiotics will usually be e ... | 2011 | 21571970 |
| haemophilus ducreyi lipooligosaccharides induce expression of the immunosuppressive enzyme indoleamine 2,3-dioxygenase via type 1 interferons and tnf-{alpha} in human dendritic cells. | haemophilus ducreyi causes chancroid, a genital ulcer disease. in human inoculation experiments, most volunteers fail to clear the bacteria despite the infiltration of innate and adaptive immune cells to the infected sites. the immunosuppressive protein indoleamine 2,3-dioxygenase (ido) is a rate-limiting enzyme in the l-tryptophan-kynurenine metabolic pathway. tryptophan depletion and tryptophan metabolites contribute to pathogen persistence by inhibiting t cell proliferation, inducing t cell a ... | 2011 | 21576329 |
| a haemophilus ducreyi cpxr deletion mutant is virulent in human volunteers. | haemophilus ducreyi 35000hp contains a homolog of the cpxra 2-component signal transduction system, which controls the cell envelope stress response system in other gram-negative bacteria and regulates some important h. ducreyi virulence factors. a h. ducreyi cpxr mutant was compared with its parent for virulence in the human challenge model of experimental chancroid. the pustule formation rate in 5 volunteers was 33% (95% confidence interval [ci], 1.3%-65.3%) at 15 parent sites and 40% (95% ci, ... | 2011 | 21606544 |
| high prevalence of hiv-1, hiv-2 and other sexually transmitted infections among women attending two sexual health clinics in bissau, guinea-bissau, west africa. | the objective was to examine the prevalence of hiv-1, hiv-2 and 10 other sexually transmitted infections (stis), and to explore the relationship between hiv and those stis in women attending two sexual health clinics in bissau, guinea-bissau. in all, 711 women with urogenital problems were included. clinical examination was performed and hiv-1, hiv-2, human t-cell lymphotropic virus (htlv)-1, htlv-2 and syphilis were diagnosed by serology. trichomonas vaginalis was examined using wet mount micro ... | 2010 | 21097735 |
| use of signature-tagged mutagenesis to identify virulence determinants in haemophilus ducreyi responsible for ulcer formation. | elucidating the molecular mechanisms responsible for chancroid, a genital ulcer disease caused by haemophilus ducreyi, has been hampered in part by the relative genetic intractability of the organism. a whole genome screen using signature-tagged mutagenesis in the temperature-dependent rabbit model (tdrm) of h. ducreyi infection uncovered 26 mutants with a presumptive attenuated phenotype. insertions in two previously recognized virulence determinants, hgba and lspa1, validated this genome scann ... | 2010 | 21185881 |
| using mathematical modelling to estimate the impact of periodic presumptive treatment on the transmission of sexually transmitted infections and hiv among female sex workers. | in settings with poor sexually transmitted infection (sti) control in high-risk groups, periodic presumptive treatment (ppt) can quickly reduce the prevalence of genital ulcers, neisseria gonorrhoeae (ng) and chlamydia trachomatis (ct). however, few studies have assessed the impact on hiv. mathematical modelling is used to quantify the likely hiv impact of different ppt interventions. | 2010 | 19854700 |
| etiology and determinants of sexually transmitted infections in karnataka state, south india. | syndromic case management remains the cornerstone for sti (sexually transmitted infection) treatment in many countries. we undertook this study to better understand the etiology of stis in adults in south india and to inform sti management guidelines. | 2010 | 19901864 |
| host-pathogen interplay of haemophilus ducreyi. | haemophilus ducreyi, the causative agent of the sexually transmitted infection chancroid, is primarily a pathogen of human skin. during infection, h. ducreyi thrives extracellularly in a milieu of professional phagocytes and other antibacterial components of the innate and adaptive immune responses. this review summarizes our understanding of the interplay between this pathogen and its host that leads to development and persistence of disease. | 2010 | 19918177 |
| bacterial intoxication evokes cellular senescence with persistent dna damage and cytokine signalling. | cytolethal distending toxins (cdts) are proteins produced and secreted by facultative pathogenic strains of gram-negative bacteria with potentially genotoxic effects. mammalian cells exposed to cdts undergo cell type-dependent cell-cycle arrest or apoptosis; however, the cell fate responses to such intoxication are mechanistically incompletely understood. here we show that both normal and cancer cells (bj, imr-90 and wi-38 fibroblasts, hela and u2-os cell lines) that survive the acute phase of i ... | 2010 | 19650831 |
| haemophilus ducreyi sapa contributes to cathelicidin resistance and virulence in humans. | haemophilus ducreyi is an extracellular pathogen of human epithelial surfaces that resists human antimicrobial peptides (aps). the organism's genome contains homologs of genes sensitive to antimicrobial peptides (sap operon) in nontypeable haemophilus influenzae. in this study, we characterized the sap-containing loci of h. ducreyi 35000hp and demonstrated that sapa is expressed in broth cultures and h. ducreyi-infected tissue; sapa is also conserved among both class i and class ii h. ducreyi st ... | 2010 | 20086092 |
| toxic activity of the cdtb component of haemophilus ducreyi cytolethal distending toxin expressed from an adenovirus 5 vector. | the haemophilus ducreyi cytolethal distending toxin (hdcdt) catalytic subunit cdtb has dnase-like activity and mediates dna damage after its delivery into target cells. we constructed a replication-deficient adenovirus type 5 (ad5) vector expressing cdtb and investigated the toxic properties of this vector on hela cells. ad5cdtb caused loss of cell viability, morphologic changes, and cell cycle arrest, findings similar to hdcdt intoxication. this confirmed that cdtb is responsible for the toxici ... | 2010 | 20132178 |
| chronic cutaneous ulcers secondary to haemophilus ducreyi infection. | haemophilus ducreyi is a well recognised causative agent of genital ulcers and chancroid. we report two unusual cases of non-sexually transmitted h. ducreyi infection leading to chronic lower limb ulcers. both patients were australian expatriates visiting australia from the pacific islands--one from papua new guinea and the other from vanuatu. | 2010 | 20230355 |
| triblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection. | rapid and simple analysis for the multiple target pathogens is critical for patient management. ce-sscp analysis on a microchip provides high speed, high sensitivity, and a portable genetic analysis platform in molecular diagnostic fields. the capability of separating ssdna molecules in a capillary electrophoretic microchannel with high resolution is a critical issue to perform the precise interpretation in the electropherogram. in this study, we explored the potential of poly(ethyleneoxide)-pol ... | 2010 | 20309929 |
| fe-heme structure in cu, zn superoxide dismutase from haemophilus ducreyi by x-ray absorption spectroscopy. | we have carried out an x-ray absorption spectroscopy (xas) study of ferric, ferrous, co- and no-bound haemophilus ducreyi cu,znsod (hdsod) in solution to investigate the structural modifications induced by the binding of small gaseous ligands to heme in this enzyme. the combined analysis of exafs and xanes data has allowed us to characterize the local structure around the fe-heme with 0.02a accuracy, revealing a heterogeneity in the distances between iron and the two histidine ligands which was ... | 2010 | 20346910 |