Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| o-heterocyclic derivatives with antibacterial properties from marine bacterium bacillus subtilis associated with seaweed, sargassum myriocystum. | the brown seaweed, sargassum myriocystum associated with heterotrophic bacterium, bacillus subtilis mtcc 10407 (jf834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio vulnificus, and vibrio parahaemolyticus. b. subtilis mtcc 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. using bioassay-guided fractionation, t ... | 2017 | 27624095 |
| transcriptome analysis reveals comprehensive insights into the early immune response of large yellow croaker (larimichthys crocea) induced by trivalent bacterial vaccine. | vaccination is an effective and safe strategy for combating bacterial diseases in fish, but the mechanisms underlying the early immune response after vaccination remain to be elucidated. in the present study, we used rna-seq technology to perform transcriptome analysis of spleens from large yellow croaker (larimichthys crocea) induced by inactivated trivalent bacterial vaccine (vibrio parahaemolyticus, vibrio alginolyticus and aeromonas hydrophila). a total of 2,789 or 1,511 differentially expre ... | 2017 | 28135311 |
| induction of type i interferons in response to bacterial stimuli in large yellow croaker larimichthys crocea. | in addition to the crucial roles in coordinating antiviral immune responses, type i interferons (ifns) also play a role in the host immunity against bacterial pathogens. our previous study identified two type i ifns from large yellow croaker larimichthys croaea(lc), lcifnd and lcifnh, and showed their strong induction by poly(i:c) and antiviral activities. in the present study, both lcifnd and lcifnh were found to be rapidly induced in head kidney and spleen by mixed bacteria of vibrio alginolyt ... | 2017 | 28119146 |
| kinneretia thg-sqi4 mediated biosynthesis of silver nanoparticles and its antimicrobial efficacy. | simple, facile, effective approach for the biosynthesis of silver nanoparticles using kinneretia species and its antimicrobial activity against human pathogens has been demonstrated in this study. kinneretia thg-sqi4 has been isolated from soil sample collected from shangqui, china. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry (uv-vis), field emission transmission electron microscopy (fe-tem), energy dispersive x-ray spectroscopy (edx), elemental mapp ... | 2017 | 28211298 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| biosynthesis of silver nanoparticles by novosphingobium sp. thg-c3 and their antimicrobial potential. | the present study described biosynthesis of silver nanoparticles (agnps) using a bacterial strain novosphingobium sp. thg-c3, isolated from soil, and their application in antibacterial activity. the maximum absorbance values of the synthesized agnps was measured at 406 nm in ultraviolet-visible spectrophotometry and were mostly spherical in shape with particle size in range of 8-25 nm by field emission transmission electron microscopy analysis. x-ray diffraction pattern corresponding to planes ( ... | 2017 | 27145847 |
| biological therapeutics of pongamia pinnata coated zinc oxide nanoparticles against clinically important pathogenic bacteria, fungi and mcf-7 breast cancer cells. | the overuse of antimicrobics and drugs has led to the development of resistance in a number of pathogens and parasites, which leads to great concerns for human health and the environment. furthermore, breast cancer is the second most common cause of cancer death in women. mcf-7 is a widely used epithelial cancer cell line, derived from breast adenocarcinoma for in vitro breast cancer studies, since the cell line has retained several ideal characteristics particular to the mammary epithelium. in ... | 2017 | 28115262 |
| control of biofilm forming clinically important bacteria by green synthesized zno nanoparticles and its ecotoxicity on ceriodaphnia cornuta. | zinc oxide nanoparticles were synthesized using the aqueous leaf extracts of plectranthus barbatus (plb-zno nps) and characterized by uv-visible spectroscopy, xrd, ftir, sem and eds. uv-visible spectra recorded the absorbance peak of plb-zno nps at 343 nm. sem analyses showed the spherical shape of plb-zno nps with the particle size between 30 and 60 nm. plb-zno nps exhibited antibacterial and antibiofilm activity against gram positive bacillus subtilis at all tested concentrations. in contrast, ... | 2017 | 28330748 |
| purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties. | the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ... | 2017 | 28110033 |
| plesiomonas shigelloides periprosthetic knee infection after consumption of raw oysters. | periprosthetic infections are a leading cause of morbidity after total joint arthroplasty. common pathogens include staphylococcus aureus, streptococcus, enterococcus, escherichia coli, and pseudomonas aeruginosa. however, there are many cases in which rare bacteria are isolated. this case report describes a periprosthetic knee infection caused by plesiomonas shigelloides. in the united states, p shigelloides and 2 other vibrionaceae family members, vibrio vulnificus and vibrio parahaemolyticus, ... | 2017 | 28235124 |
| microbiological quality and prevalence of β-lactam antibiotic resistance genes in oysters ( crassostrea rhizophorae ). | the microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. the objective of this study was to assess the bacterial composition of the edible oyster crassostrea rhizophorae in urban and preserved estuaries. particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blatem, blashv, and blakpc) via quantitative pcr ... | 2017 | 28207310 |
| vibrio phage kvp40 encodes a functional nad+ salvage pathway. | the genome of t4-type vibrio bacteriophage kvp40 has five genes predicted to encode proteins of pyridine nucleotide metabolism, of which two, nadv and natv, would suffice for an nad(+) salvage pathway. nadv is an apparent nicotinamide phosphoribosyltransferase (namprtase) and natv is an apparent bifunctional nicotinamide mononucleotide adenylyltransferase (nmnatase) and nicotinamide-adenine dinucleotide pyrophosphatase (nudix hydrolase). genes encoding the predicted salvage pathway were cloned, ... | 2017 | 28167526 |
| debaryomyces hansenii up regulates superoxide dismutase gene expression and enhances the immune response and survival in pacific red snapper (lutjanus peru) leukocytes after vibrio parahaemolyticus infection. | application of yeast is increasing to improve welfare and promotes growth in aquaculture. the halotolerant yeast debaryomyces hansenii is normally a non-pathogenic yeast with probiotic properties and potential source of antioxidant enzymes as superoxide dismutase. here, first, we characterized the sequence features of mnsod and iccu/znsod from pacific red snapper, and second, we evaluated the potential antioxidant immune responses of the marine yeast debaryomyces hansenii strain cbs004 in leukoc ... | 2017 | 28126556 |
| molecular identification and functional delineation of a glutathione reductase homolog from disk abalone (haliotis discus discus): insights as a potent player in host antioxidant defense. | glutathione reductase (gsr) is an enzyme that catalyzes the biochemical conversion of oxidized glutathione (gssg) into the reduced form (gsh). since the ratio between the two forms of glutathione (gsh/gssg) is important for the optimal function of gsh to act as an antioxidant against h2o2, the contribution of gsr as an enzymatic regulatory agent to maintain the proper ratio is essential. abalones are marine mollusks that frequently encounter environmental factors that can trigger the overproduct ... | 2017 | 27919756 |
| tc38, a teleost tfpi-2 peptide that kills bacteria via penetration of the cell membrane and interaction with nucleic acids. | tissue factor pathway inhibitor 2 (tfpi-2) is an analog of tfpi-1 and a potent endogenous inhibitor of tissue factor (tf)-mediated blood coagulation. recent reports have proven that the c-terminal of tfpi-2 peptides in humans and several other vertebrates possesses antibacterial activity against gram-positive and gram-negative bacteria. in our previous study, we reported that the tfpi-2 peptide, tc38 in tongue sole (cynoglossus semilaevis) was active against micrococcus luteus. in this study, we ... | 2017 | 28263880 |
| identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn exopalaemon carinicauda. | anti-lipopolysaccharide factor is a kind of antimicrobial peptide (amp) with broad-spectrum activities against bacteria, virus, and fungi in crustacean. different isoforms of alfs showed different activities to virus or bacteria. therefore, discovery of more alfs will provide new insights into drug development and disease control. here we reported an alf gene, ecalf1, isolated from the ridgetail prawn exopalaemon carinicauda. its transcripts was mainly detected in hemocytes. silencing of ecalf1 ... | 2017 | 28082184 |
| mechanisms for pseudoalteromonas piscicida-induced killing of vibrios and other bacterial pathogens. | pseudoalteromonas piscicida are gram-negative gammaproteobacteria found in the marine environment. three strains of pigmented p. piscicida were isolated from seawater and partially characterized by inhibition studies, electron microscopy, and proteolytic enzyme production. growth inhibition and death occurred around colonies of p. piscicida on lawns of the naturally-occurring marine pathogens vibrio vulnificus, v. parahaemolyticus, v. cholerae, photobacterium damselae and shewanella algae inhibi ... | 2017 | 28363962 |
| dietary yeast sterigmatomyces halophilus enhances mucosal immunity of gilthead seabream (sparus aurata l.). | a yeast was isolated from hypersaline sediments, grown and phylogenetically characterized as sterigmatomyces halophilus strainn16. the dietary administration of this yeast was studied for its effect on skin mucosal immune and antioxidant status of gilthead seabream (sparus aurata l.). fish were fed a commercial diet (control, non-supplemented diet), or the same commercial diet supplemented with 0.55% or 1.1% of yeast for 15 and 30 days. one month after the end of the trial, fish from all treatme ... | 2017 | 28315392 |
| toxicity of herbal extracts used in ethno-veterinary medicine and green-encapsulated zno nanoparticles against aedes aegypti and microbial pathogens. | dengue and chikungunya are arboviral diseases mainly vectored by the mosquito aedes aegypti. presently, there is no treatment for these viral diseases and their prevention is still based on vector control measures. nanopesticides fabricated using herbal extracts as reducing and capping agents currently represent an excellent platform for pest control. in this scenario, the present study assessed the acute toxicity of seven plants employed in ethno-veterinary medicine of southern india, as well a ... | 2017 | 28389893 |
| an outbreak of shigellosis in a children welfare institute caused by a multiple-antibiotic-resistant strain of shigella flexneri 2a. | from september 1 to october 27, 2015, an outbreak of bacillary dysentery occurred in the shenzhen children welfare institute (scwi). the shigellosis was uncommon in shenzhen and no related outbreak was reported during the last 5 years. an epidemiological investigation was conducted and the children and nursing workers in scwi were surveyed for gastrointestinal symptoms; 28 of children reported having a diarrheal illness. rectal swabs or fecal specimens from 14 case patients and 24 nursing worker ... | 2017 | 28189512 |
| mice with streptozotocin-induced hyperglycemia are susceptible to invasive enteric bacterial infection. | diabetes mellitus and diarrhea are becoming increasingly burdensome worldwide, particularly in developing countries such as india. diabetic patients are susceptible to infection with pathogenic bacteria, particularly those causing invasive enteric infections. in this study, we observed changes in the pathophysiological features of mice with streptozotocin-induced hyperglycemia. in our experiments, both hyperglycemic and control mice were infected with pathogenic enteric bacteria-non-typhoidal sa ... | 2017 | 27169940 |
| a novel anti-lipopolysaccharide factor spalf6 in mud crab scylla paramamosain exhibiting different antimicrobial activity from its single amino acid mutant. | in crustaceans, anti-lipopolysaccharide factors (alfs) are important immune effectors that have sequence diversity and exhibit broad antimicrobial activities. in this study, we characterized a novel alf homolog spalf6 from mud crab scylla paramamosain and its variant spalf6-v, which was generated by mutations of two amino acids (h46 to r and a110 to p) due to the presence of two single nucleotide polymorphisms (snps). spalf6 was an anionic peptide with isoelectric point (pi) 6.79, whereas spalf6 ... | 2017 | 28232132 |
| hctoll3 was involved in anti-vibrio defense in freshwater pearl mussel, hyriopsis cumingii. | toll-like receptors (tlrs) play an important role in the activation of innate immune response but their functions in bivalves remain largely unknown. in this study, we identified a tlr from the freshwater pearl mussel hyriopsis cumingii (hctoll3) and investigated its functions in immunity. the full-length cdna of hctoll3 is 3852 bp and includes an open reading frame (orf) of 3228 bp that encodes a polypeptide of 1075 amino acids. the predicted hctoll3 protein shares similar structural characteri ... | 2017 | 28214599 |
| identification and functional analysis of a tep gene from a crustacean reveals its transcriptional regulation mediated by nf-κb and jnk pathways and its broad protective roles against multiple pathogens. | thioester-containing proteins (teps) are present in a wide range of species from deuterostomes to protostomes and are thought to be involved in innate immunity. in the current study, a tep gene homologous to insect teps (itep) from the crustacean litopenaeus vannamei, named lvtep1, is cloned and functionally characterized. the open reading frame (orf) of lvtep1 is 4383 bp in length, encoding a polypeptide of 1460 amino acids with a calculated molecular weight of 161.1 kda lvtep1, which is most s ... | 2017 | 28069434 |
| designation of pathogenic resistant bacteria in the sparusaurata sea collected in tunisia coastlines: correlation with high performance liquid chromatography-tandem mass spectrometry analysis of antibiotics. | vibrio is characterized by a large number of species and some of them are human pathogens causing gastro intestinal and wound infections through the ingestion or manipulation of contaminated fishes including vibrio parahaemolyticus and vibrio alginolyticus. in this study, we reported the phenotypic and molecular characterization of vibrio parahaemolyticus and vibrio alginolyticus strains isolated from wild and farm sea bream (sparus aurata l.) along the tunisian coast from december 2015 to april ... | 2017 | 28062288 |
| season-specific occurrence of potentially pathogenic vibrio spp. on the southern coast of south korea. | vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. in this study, we aimed to isolate and observe the distribution of enteropathogenic vibrio spp. from environments of the southern coast of south korea over a season cycle. a total of 10,983 isolates of vibrio spp. were obtained from tidal water and mud samples over a 1-year period from five sampling sites along the southw ... | 2017 | 27836844 |
| biogeochemical and hydrological drivers of the dynamics of vibrio species in two patagonian estuaries. | the ecology of the most relevant vibrio species for human health and their relation to water quality and biogeochemistry were studied in two estuaries in argentinian patagonia. vibrio cholerae and vibrio parahaemolyticus were reported in >29% of cases at the río colorado and río negro estuaries. neither the pandemic serogroups of vibrio cholerae o1, vibrio cholerae o139 nor the cholera toxin gene were detected in this study. however, several strains of v. cholerae (not o1 or o139) are able to ca ... | 2017 | 27871750 |
| non-cholera vibrios: the microbial barometer of climate change. | there is a growing interest in the role of climate change in driving the spread of waterborne infectious diseases, such as those caused by bacterial pathogens. one particular group of pathogenic bacteria - vibrios - are a globally important cause of diseases in humans and aquatic animals. these gram-negative bacteria, including the species vibrio vulnificus, vibrio parahaemolyticus and vibrio cholerae, grow in warm, low-salinity waters, and their abundance in the natural environment mirrors ambi ... | 2017 | 27843109 |
| responses of mytilus galloprovincialis hemocytes to environmental strains of vibrio parahaemolyticus, vibrio alginolyticus, vibrio vulnificus. | marine bivalves are exposed to different types of bacteria in the surrounding waters, in particular of the vibrio genus. in the hemocytes of the mussel mytilus spp. immune responses to different vibrios have been largely characterized. however, little information is available on the hemocyte responses to human pathogenic vibrios commonly detected in coastal waters and bivalve tissues that are involved in seafood-borne diseases. | 2017 | 28390964 |
| immune response and antioxidant status of portunus trituberculatus inoculated with pathogens. | the white spot syndrome virus (wssv), vibrio parahaemolyticus and v. alginolyticus are serious epidemic pathogen affecting the cultured portunus trituberculatus and resulted in severe economic losses to local farmers. the immune and antioxidant systems are believed to be closely involved in host responses to pathogens in aerobic animals, including crustaceans. in order to explore such host-pathogen interactions in the early stage of infection in p. trituberculatus, the mrna transcript levels of ... | 2017 | 28235638 |
| shrimp ahpnd-causing plasmids encoding the pirab toxins as mediated by pirab-tn903 are prevalent in various vibrio species. | acute hepatopancreatic necrosis disease (ahpnd) is a newly emerging shrimp disease caused by pirab toxins encoded by a plasmid found in vibrio parahaemolyticus. the pirab toxins are the homologs of the photorhabdus insect-related (pir) toxins. here, we report the complete sequences of the ahpnd-causing plasmid isolated from v. owensii, as well as those of its 11 siblings (pvh family). in addition, we also included 13 related plasmids (pvh-r family) without the pirab genes isolated from a variety ... | 2017 | 28169338 |
| calr is required for the expression of t6ss2 and the adhesion of vibrio parahaemolyticus to hela cells. | vibrio parahaemolyticus expresses one major virulence determinant t6ss2, which is constituted into three putative operons, i.e., vpa1027-1024, vpa1043-1028, and vpa1044-1046. calr, a lysr-type transcriptional regulator, was originally identified as a repressor of the swarming motility and t3ss1 gene expression. as shown in this study, calr binds to the promoter-proximal region of each of the three operons to activate their transcription, and moreover, calr activates the adhesion of v. parahaemol ... | 2017 | 28378143 |
| kinetics of the inactivation of vibrio parahaemolyticus in weakly acidic sodium chlorite solution. | the kinetics of the inactivation of vibrio parahaemolyticus in sodium chlorite (naclo2) solution was studied in the weakly acidic ph range of 4.0 to 6.5 and at various temperatures. the logarithmic reduction of the survival ratio depended on the concentration-time product, and all the inactivation curves showed a linear reduction phase. the first-order inactivation rate constant (k) increased by approximately twice for every 0.44 unit fall in ph. during the inactivation experiments, no formation ... | 2017 | 28367867 |
| development of a multiplex loop-mediated isothermal amplification method for the simultaneous detection of salmonella spp. and vibrio parahaemolyticus. | rapid detection of food-borne pathogens is important in the food industry, to monitor and prevent the spread of these pathogens through contaminated food products. we therefore established a multiplex real-time loop-mediated isothermal amplification (lamp) assay to simultaneously detect and distinguish salmonella spp. and vibrio parahaemolyticus dna in a single reaction. two target sequences, one specific for salmonella and the other specific for vibrio parahaemolyticus, were amplified by specif ... | 2017 | 28349967 |
| defining a core genome multilocus sequence typing scheme for the global epidemiology of vibrio parahaemolyticus. | vibrio parahaemolyticus is an important human foodborne pathogen whose transmission is associated with the consumption of contaminated seafood with a growing number of infections reported over recent years worldwide. a multilocus sequence typing (mlst) database for v. parahaemolyticus was created in 2008 and a large number of clones have been identified causing severe outbreaks worldwide (st3), recurrent outbreaks in certain regions (e.g., st36) or spreading to other regions where they are non-e ... | 2017 | 28330888 |
| transcriptional regulation of cpsq-mfpabc and mfpabc by calr in vibrio parahaemolyticus. | the cpsq-mfpabc locus is transcribed as two operons, i.e., cpsq-mfpabc and mfpabc, in vibrio parahaemolyticus, and both of them are all required for biofilm formation. calr belongs to the lysr-type transcriptional regulator family, and was originally identified as a repressor of the swarming motility and t3ss1 genes expression in v. parahaemolyticus. in the present work, a combination of qrt-pcr, primer extension, lacz fusion expression, electrophoretic mobility shift assay, and dnase i footprin ... | 2017 | 28318117 |
| characterization of vibrio parahaemolyticus isolated from oysters in korea: resistance to various antibiotics and prevalence of virulence genes. | vibrio parahaemolyticus, found frequently in oysters, is the most prevalent gastroenteritis-causing pathogen in korea and in several other asian countries. this study monitored changes in the environmental parameters and occurrence of v. parahaemolyticus in oyster aquaculture sites. of the 44 presumed v. parahaemolyticus isolates obtained, when tested against 16 antibiotics, 90.9, 86.4, and 75.0% of the 44 isolates exhibited resistance to vancomycin, ampicillin, and streptomycin, respectively. p ... | 2017 | 28279505 |
| vibrio parahaemolyticus calr down regulates the thermostable direct hemolysin (tdh) gene transcription and thereby inhibits hemolytic activity. | tdh, encoded by tdh gene, is a major virulent determinant of v. parahaemolyticus that controls various biological activities, such as hemolytic activity, cytotoxicity, and enterotoxicity. the hemolytic activity on wagatsuma agar ascribed to tdh is called kanagawa phenomenon (kp). all kp positive strains contain tdh1 and tdh2 genes, but tdh2 is predominantly responsible for kp. calr is a regulatory protein that was originally identified as a repressor of swarming motility and t3ss1 gene expressio ... | 2017 | 28268179 |
| an invertebrate sting from shrimp activates an innate immune defense against bacterial infection. | it has been proposed that invertebrate stimulators of interferon genes (stings) do not take part in the innate immune response to infection. herein, we identified a new sting homolog from pacific white shrimp litopenaeus vannamei (lvsting). some amino acids crucial for recognizing cyclic dinucleotides in mammals are highly conserved in lvsting. moreover, lvsting expression can be robustly induced by challenge with the gram-negative bacteria vibrio parahaemolyticus. silencing of lvsting contribut ... | 2017 | 28236646 |
| a simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus. | an earlier electrochemical mechanism of dna detection was adapted and specified for the detection of vibrio parahaemolyticus in real samples. the reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. detection was assessed using a microprocessor to measure current response under controlled potential. the fabricated sensor was able to specifically distinguish complementary, non-comple ... | 2017 | 28205102 |
| ecological fitness and virulence features of vibrio parahaemolyticus in estuarine environments. | vibrio parahaemolyticus is a commonly encountered and highly successful organism in marine ecosystems. it is a fast-growing, extremely versatile copiotroph that is active over a very broad range of conditions. it frequently occurs suspended in the water column (often attached to particles or zooplankton), and is a proficient colonist of submerged surfaces. this organism is an important pathogen of animals ranging from microcrustaceans to humans and is a causative agent of seafood-associated food ... | 2017 | 28144705 |
| genetic and serological identification of three vibrio parahaemolyticus strains as candidates for novel provisional o serotypes. | vibrio parahaemolyticus is a gram-negative, halophilic vibrio that naturally inhabits marine and estuarine environments worldwide and has recently been recognized as one of the most important foodborne pathogens. to date, 13 o serotypes and 71 k serotypes of v. parahaemolyticus have been identified. however, untypeable v. parahaemolyticus strains are frequently found during routine detection, indicating that other forms of serotypes exist and suggesting the necessity for extension of the antigen ... | 2017 | 28129570 |
| detection, enumeration, and isolation of vibrio parahaemolyticus and v. vulnificus from seafood: development of a multidisciplinary protocol. | vibrio parahaemolyticus and v. vulnificus are bacterial foodborne pathogens that can cause illnesses in humans after ingestion or exposure to contaminated seafood or coastal waters. a procedure that combines microbiological, biochemical, and molecular methods was designed and optimized for the detection, enumeration, isolation, and characterization of these clinically significant vibrio spp. initially, microbiological culturing is used to resuscitate and isolate presumptive vibrio spp. from chil ... | 2017 | 28118133 |
| transcriptome profiles of the clam meretrix petechialis hepatopancreas in response to vibrio infection. | microbial diseases have received much attention due to their enormous destruction of aquaculture, and vibrio parahaemolyticus is one of the main pathogens that cause bacterial disease in the clam meretrix petechialis. to better understand the molecular mechanisms of the immune response to vibrio in m. petechialis, rna-seq was applied to explore global expression changes of hepatopancreas from this clam after vibrio challenge. there were 199,318,966 clean reads obtained by illumina sequencing, wh ... | 2017 | 28110034 |
| high salinity relaying to reduce vibrio parahaemolyticus and vibrio vulnificus in chesapeake bay oysters (crassostrea virginica). | cases of vibrio infections in the united states have tripled from 1996 to 2009 and these infections are most often associated with the consumption of seafood, particularly oysters (crassostrea virginica). information is needed on how to reduce numbers of vibrio parahaemolyticus and vibrio vulnificus in bi-valve molluscan shellfish (for example, oysters). the purpose of this study was to evaluate the effectiveness of high salinity relaying or treatment in recirculating aquaculture systems (rass) ... | 2017 | 28099766 |
| genetic analysis of vibrio parahaemolyticus o3:k6 strains that have been isolated in mexico since 1998. | vibrio parahaemolyticus is an important human pathogen that has been isolated worldwide from clinical cases, most of which have been associated with seafood consumption. environmental and clinical toxigenic strains of v. parahaemolyticus that were isolated in mexico from 1998 to 2012, including those from the only outbreak that has been reported in this country, were characterized genetically to assess the presence of the o3:k6 pandemic clone, and their genetic relationship to strains that are r ... | 2017 | 28099500 |
| establishment and validation of rna-based predictive models for understanding survival of vibrio parahaemolyticus in oysters stored at low temperatures. | this study developed rna-based predictive models describing the survival of vibrio parahaemolyticus in eastern oysters (crassostrea virginica) during storage at 0, 4, and 10°c. postharvested oysters were inoculated with a cocktail of five v. parahaemolyticus strains and were then stored at 0, 4, and 10°c for 21 or 11 days. a real-time reverse transcription-pcr (rt-pcr) assay targeting expression of the tlh gene was used to evaluate the number of surviving v. parahaemolyticus cells, which was the ... | 2017 | 28087532 |
| vibrio parahaemolyticus o4:k8 forms a potential predominant clone in southern china as detected by whole-genome sequence analysis. | vibrio parahaemolyticus has been the most common food-borne pathogen in southern china, especially the o3:k6 pandemic clone and its serovariants. recently, the serotype o4:k8 became more and more prevalent in southern china, which was different from the o3:k6 pandemic clone. thus, the aim of the present work was to elucidate the molecular characteristics of the o4:k8. some o3:k6 pandemic clone and its serovariants isolated in the same period were selected for comparative analysis, which were sti ... | 2017 | 28073082 |
| quorum sensing regulators are required for metabolic fitness in vibrio parahaemolyticus. | quorum sensing (qs) is a process by which bacteria alter gene expression in response to cell density changes. in vibrio species, at low cell density, the sigma 54-dependent response regulator luxo is active and regulates the two qs master regulators apha, which is induced, and opar, which is repressed. at high cell density the opposite occurs: luxo is inactive, and therefore opar is induced while apha is repressed. in vibrio parahaemolyticus, a significant enteric pathogen of humans, the roles o ... | 2017 | 28069817 |
| occurrence and virulence properties of vibrio and salinivibrio isolates from tropical lagoons of the southern caribbean sea. | the vibrionaceae are gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. the aim of this research was to investigate the occurrence and virulence properties of vibrio and salinivibrio isolated from lagoons at cuare wildlife refuge and margarita island in the southern caribbean sea. water, plankton and oyster samples were collected during october 2011 and march 2012 and examined by s ... | 2017 | 28324231 |
| biochemical basis for activation of virulence genes by bile salts in vibrio parahaemolyticus. | bile salts act as a stressor to bacteria that transit the intestinal tract. enteric pathogens have hijacked bile as an intestinal signal to regulate virulence factors. we recently demonstrated that vibrio parahemolyticus senses bile salts via a heterodimeric receptor formed by the periplasmic domains of inner-membrane proteins vtra and vtrc. crystal structures of the periplasmic complex reveal that vtra and vtrc form a β-barrel that binds bile salts in its hydrophobic interior to activate the vt ... | 2017 | 28129014 |
| an integrated rotary microfluidic system with dna extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics. | point-of-care (poc) molecular diagnostics plays a pivotal role for the prevention and treatment of infectious diseases. in spite of recent advancement in microfluidic based poc devices, there are still rooms for development to realize rapid, automatic and cost-effective sample-to-result genetic analysis. in this study, we propose an integrated rotary microfluidic system that is capable of performing glass microbead based dna extraction, loop mediated isothermal amplification (lamp), and colorime ... | 2017 | 28043075 |
| alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp litopenaeus vannamai. | the shrimp multifunctional protein alpha-2-macroglobulin (a2m) is abundantly expressed in plasma, highly up-regulated upon microbial infection and involved in several immune pathways such as blood clotting system, phagocytosis and melanization. herein, the function of lva2m from litopenaeus vannamei on the prophenoloxidase (propo) system is reported. the recombinant (r)lva2m produced strongly and specifically inhibited trypsin and the po activity in shrimp plasma in a dose-dependent manner. sile ... | 2017 | 28027986 |
| sequence type 631 vibrio parahaemolyticus, an emerging foodborne pathogen in north america. | 2017 | 27974540 | |
| molecular cloning and expression analysis of a fish specific interferon regulatory factor, irf11, in orange spotted grouper, epinephelus coioides. | interferon regulatory factors (irfs) are transcription mediators which play vital roles in multiple biological processes, such as antiviral defense, immune response, cell growth regulation and apoptosis. a fish specific irf, termed irf11, has been identified in previous study through searching fish genome databases. herein, a transcript of irf11, ecirf11 was cloned from orange-spotted grouper, epinephelus coioides. the ecirf11 cdna sequence has 1573 bp in length, encoding a putative protein of 2 ... | 2017 | 27932023 |
| sensitive immunoassay-based detection of vibrio parahaemolyticus using capture and labeling particles in a stationary liquid phase lab-on-a-chip. | in the present study, a method was developed for detection of vibrio parahaemolyticus based on a stationary liquid phase lab-on-a-chip (slp loc). the present slp loc comprises a sample chamber, washing chamber, and detection chamber connected by two channels. the method utilizes two types of particles: capture particles (cps), which are magnetic nanoparticles functionalized with antibody; and labeling particles (lps), which are silica nanoparticles functionalized with horseradish peroxidase and ... | 2017 | 27923189 |
| fatty acid synthase plays a positive role in shrimp immune responses against vibrio parahaemolyticus infection. | fatty acid synthase (fas) is an important enzyme that catalyzes the synthesis of fatty acids. in this study, the role of the fas gene from pacific white shrimp litopenaeus vannamei (lvfas) in immune responses against vibrio parahaemolyticus infection was studied. the expression of lvfas could be up-regulated upon infection of v. parahaemolyticus and stimulation of lipopolysaccharide and poly (i:c). the promoter of lvfas was predicted to harbor a nf-κb binding site and dual-luciferase reporter as ... | 2017 | 27903451 |
| metabolic responses of haliotis diversicolor to vibrio parahaemolyticus infection. | vibrio parahemolyticus is a devastating bacterial pathogen that often causes outbreak of vibriosis in abalone haliotis diversicolor. elucidation of metabolic mechanisms of abalones in responding to v. parahemolyticus infection is essential for controlling the epidemic. in this work, (1)h nmr-based metabolomic techniques along with correlation and network analyses are used to investigate characteristic metabolites, as well as corresponding disturbed pathways in hepatopancreas and gill of h. diver ... | 2017 | 27890800 |
| enhancing gilthead seabream immune status and protection against bacterial challenge by means of antigens derived from vibrio parahaemolyticus. | in an attempt to control the proliferation of the pathogenic bacterium vibrio parahaemolyticus in gilthead seabream (sparus aurata), the immunostimulant effect of lysate and toxa from this bacterium was evaluated. fish were intraperitoneally injected twice (first injection, day 1 of the experiment; second injection, day 7) and sampled after one week (on days 8 and 15). afterwards, all fish specimens were experimentally infected with v. parahaemolyticus and mortality was recovered for 1 week. fis ... | 2017 | 27890799 |
| the role of ficolin-like protein (pcflp1) in the antibacterial immunity of red swamp crayfish (procambarus clarkii). | in invertebrates, ficolin-like proteins (flps) play important roles in innate immunity against pathogens. previous studies primarily investigated the functions of flps in immune recognition, activation, and regulation. however, limited research has examined the functions of flps as immune effectors. in this work, a ficolin-like protein was identified in red swam crayfish (procambarus clarkii) and designated as pcflp1. quantitative rt-pcr and western blot were employed to analyze the distribution ... | 2017 | 27888717 |
| hsp70 and hsp90 are involved in shrimp penaeus vannamei tolerance to ahpnd-causing strain of vibrio parahaemolyticus after non-lethal heat shock. | acute hepatopancreatic necrosis disease (ahpnd) caused by vibrio parahaemolyticus carrying toxin-producing plasmid, has led to severe mortalities in farmed penaeid shrimp throughout asia. previous studies reported that a non-lethal heat shock (nlhs) could enhance disease tolerance in aquatic animals. here, we investigate whether the nlhs could enhance the survival of shrimp penaeusvannamei upon challenge with an ahpnd-causing strain of v. parahaemolyticus (vpahpnd). two nlhs conditions, acute an ... | 2017 | 27888131 |
| the hot oyster: levels of virulent vibrio parahaemolyticus strains in individual oysters. | vibrio parahaemolyticus is the leading cause of seafood-associated gastroenteritis and is most commonly transmitted by raw oysters. consequently, detection of virulent strains of this organism in oysters is a primary concern for seafood safety. vibrio parahaemolyticus levels were determined in 110 individual oysters harvested from two sampling sites in sc, usa. the majority of oysters (98%) contained low levels of presumptive v. parahaemolyticus however, two healthy oysters contained presumptive ... | 2017 | 27827805 |
| disulphide bond restrains the c-terminal region of thermostable direct hemolysin during folding to promote oligomerization. | pore-forming toxins (pfts) are typically produced as water-soluble monomers, which upon interacting with target cells assemble into transmembrane oligomeric pores. vibrio parahaemolyticus thermostable direct hemolysin (tdh) is an atypical pft that exists as a tetramer in solution, prior to membrane binding. the tdh structure highlights a core β-sandwich domain similar to those found in the eukaryotic actinoporin family of pfts. however, the tdh structure harbors an extended c-terminal region (ct ... | 2017 | 27784764 |
| impact of farm management on expression of early mortality syndrome/acute hepatopancreatic necrosis disease (ems/ahpnd) on penaeid shrimp farms in thailand. | asian shrimp farming industry has experienced massive production losses due to a disease caused by toxins of vibrio bacteria, known as early mortality syndrome/acute hepatopancreatic necrosis disease (ems/ahpnd) for the last 5 years. the disease can cause up to 100% cumulative pond mortality within a week. the objective of this study was to identify factors associated with ahpnd occurrence on shrimp farms. a case-control study was carried out on shrimp farms in four provinces of thailand. factor ... | 2017 | 27594170 |
| prevalence of vibrio parahaemolyticus in seafood products from hypermarkets in shanghai. | vibrio parahaemolyticus is an important gastroenteritis pathogen contaminating seafood in china. in this study a total of 992 seafood samples from major hypermarkets in shanghai were monitored for prevalence and burden of v. parahaemolyticus from january 2011 to december 2012. additionally, appropriate probability distributions for describing v. parahaemolyticus concentrations were assessed based on these surveillance data. | 2017 | 26991741 |
| differences in abundances of total vibrio spp., v. vulnificus, and v. parahaemolyticus in clams and oysters in north carolina. | filter feeding shellfish can concentrate pathogenic bacteria, including vibrio vulnificus and vibrio parahaemolyticus, as much as 100-fold from the overlying water. these shellfish, especially clams and oysters, are often consumed raw, providing a route of entry for concentrated doses of pathogenic bacteria into the human body. the numbers of foodborne infections with these microbes are increasing, and a better understanding of the conditions that might trigger elevated concentrations of these b ... | 2017 | 27793822 |
| [growth and survival of total and pathogenic vibrio parahaemolyticus in american oyster (crassostrea virginica) under cold storage]. | to quantify vibrio parahaemolyticus densities in american oyster (crassostrea virginica) under cold storage. | 2017 | 26302123 |
| identification and characterization of a laccase from litopenaeus vannamei involved in anti-bacterial host defense. | phenoloxidases (pos) are a family of enzymes including tyrosinases, catecholases and laccases, which play an important role in immune defences of various invertebrates. whether or not laccase exists in shrimp and its function is still poorly understood. in this study, a laccase (lvlac) was cloned and identified from litopenaeus vannamei for the first time. the full length of lvlac is 3406 bp, including a 2034 bp open reading frame (orf) coding for a putative protein of 677 amino acids with a sig ... | 2017 | 28476665 |
| identification of a macrobrachium nipponense c-type lectin with a close evolutionary relationship to vertebrate lectins. | c-type lectins (ctls) are involved in the innate immune defense of vertebrates and invertebrates against invading pathogens. this study cloned and characterized a novel c-type lectin (mnctl) of the oriental river prawn, macrobrachium nipponense. the cloned mnctl cdna encompasses an open reading frame of 774 nucleotides and encodes polypeptides of 257 residues. the deduced mnctl protein contains a single carbohydrate recognition domain (crd) with an epn (glu-pro-asn) motif in calcium-binding site ... | 2017 | 28441623 |
| vibrio parahaemolyticus- and v. alginolyticus-associated meningo-encephalitis in a bottlenose dolphin (tursiops truncatus) from the adriatic coast of italy. | a case of vibrio parahaemolyticus- and v. alginolyticus-associated meningo-encephalitis in a bottlenose dolphin (tursiops truncatus) found stranded along the adriatic coast of italy in 2016 is herein reported, along with a minireview on v. parahaemolyticus and v. alginolyticus infections in aquatic mammals. macroscopically, two abscesses were found in the dolphin's forebrain, along with an extensive, bilateral, parasitic broncho-pneumonia. histologically, a suppurative-to-pyogranulomatous mening ... | 2017 | 28709108 |
| previously undescribed antibacterial polyketides from heterotrophic bacillus amyloliquefaciens associated with seaweed padina gymnospora. | a heterotrophic marine bacterium bacillus amyloliquefaciens isolated from seaweed padina gymnospora exhibited broad spectra of antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio harveyi, vibrio vulnificus, and vibrio parahaemolyticus. the seaweed-associated b. amyloliquefaciens was recognized to possess functional type i polyketide synthase-1 (pks-1) gene, and was used to isolate four homologous compounds with polyketide frameworks. the compounds were characterized ... | 2017 | 28842846 |
| corrigendum: assessing the function of stas domain protein sypa in vibrio fischeri using a comparative analysis. | [this corrects the article on p. 760 in vol. 6, pmid: 26284045.]. | 2017 | 28450861 |
| a single wap domain-containing protein from litopenaeus vannamei possesses antiproteinase activity against subtilisin and antimicrobial activity against ahpnd-inducing vibrio parahaemolyticus. | the single wap domain-containing protein (swd) is a type iii crustin antimicrobial peptide whose function is to defense the host animal against the bacterial infection by means of antimicrobial and antiproteinase activities. a study of swd from litopenaeus vannamei (lvswd) is reported herein about its activities and function against bacteria, particularly the ahpnd-inducing vibrio parahaemolyticus (vpahpnd) that causes acute hepatopancreatic necrosis disease (ahpnd). the lvswd is mainly synthesi ... | 2017 | 28743625 |
| bioluminescence imaging to track bacteroides fragilis inhibition of vibrio parahaemolyticus infection in mice. | bacteroides fragilis is an anaerobic, gram-negative, commensal bacterium of the human gut. it plays an important role in promoting the maturation of the immune system, as well as suppressing abnormal inflammation. many recent studies have focused on the relationship between b. fragilis and human immunity, and indicate that b. fragilis has many useful probiotic effects. as inhibition of intestinal pathogens is an important characteristic of probiotic strains, this study examined whether b. fragil ... | 2017 | 28553617 |
| whole-body microbiota of sea cucumber (apostichopus japonicus) from south korea for improved seafood management. | sea cucumber (apostichopus japonicus) is a popular seafood source in asia, including south korea, and its consumption has recently increased with recognition of its medicinal properties. however, because raw sea cucumber contains various microbes, its ingestion can cause foodborne illness. therefore, analysis of the microbiota in the whole body of sea cucumber can extend our understanding of foodborne illness caused by microorganisms and help to better manage products. we collected 40 sea cucumb ... | 2017 | 28851208 |
| role of purified β-1, 3 glucan binding protein (β-gbp) from paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties. | β- 1, 3-glucan binding protein (β-gbp), a pattern recognition protein (prp), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. in the present study, β-gbp was purified from the haemolymph of rice field crab paratelphusa hydrodromus by affinity column chromatography. the monomeric protein ph-β-gbp appeared as a single band with a molecular weight of approximately 95 kda in sds-page analysis and its purity was determined to be 89 ... | 2017 | 28684323 |
| expression of a vibrio parahaemolyticus toxin in escherichia coli results in chromosomal dna degradation. | a toxin-antitoxin system, vp1842/vp1843, locates within a superintegron on the vibrio parahaemolyticus genome chromosome i whose toxin gene vp1843 encodes a dna nicking endonuclease. we found that the vp1843 expression in escherichia coli cells strongly induced chromosomal dna degradation. on the basis of these observations, we discuss a possible physiological role of vp1842/vp1843 in v. parahaemolyticus. | 2017 | 28820009 |
| ecofriendly synthesis of silver and gold nanoparticles by euphrasia officinalis leaf extract and its biomedical applications. | biogenic synthesis of silver (agnps) and gold nanoparticles (aunps) using aqueous extract of euphrasia officinalis has been reported. stable agnps and aunps were formed on adding aqueous solutions of silver nitrate and chloroauric acid with e. officinalis leaf extract, in 19 min and 2 min, respectively. the synthesis method used in present study was simple, reliable, rapid, cost effective and ecofriendly. the synthesized nanoparticles were characterized with field emission transmission electron ... | 2017 | 28784039 |
| seasonal influence of scallop culture on nutrient flux, bacterial pathogens and bacterioplankton diversity across estuaries off the bohai sea coast of northern china. | in this study, we investigated the environmental impacts of scallop culture on two coastal estuaries adjacent the bohai sea including developing a quantitative pcr assay to assess the abundance of the bacterial pathogens escherichia coli and vibrio parahaemolyticus. scallop culture resulted in a significant reduction of nitrogen, chlorophyll a, and phosphorous levels in seawater during summer. the abundance of bacteria including v. parahaemolyticus varied significantly across estuaries and breed ... | 2017 | 28779889 |
| removal of foodborne pathogen biofilms by acidic electrolyzed water. | biofilms, which are complex microbial communities embedded in the protective extracellular polymeric substances (eps), are difficult to remove in food production facilities. in this study, the use of acidic electrolyzed water (aew) to remove foodborne pathogen biofilms was evaluated. we used a green fluorescent protein-tagged escherichia coli for monitoring the efficiency of aew for removing biofilms, where under the optimal treatment conditions, the fluorescent signal of cells in the biofilm di ... | 2017 | 28638370 |
| a rele/pare superfamily toxin in vibrio parahaemolyticus has dna nicking endonuclease activity. | type ii toxins in toxin-antitoxin (ta) systems fold into a similar fold and belong to the rele/pare superfamily. however, they display two distinct biochemical activities: rele toxins are mrna interferases, while pare toxins are dna gyrase (gyr) inhibitors. previously, we found a ta system, vp1842/vp1843, on the vibrio parahaemolyticus genome whose toxin vp1843 belongs to the rele/pare toxin superfamily. vp1843, unlike rele toxins, has neither protein synthesis inhibitory activity nor ribonuclea ... | 2017 | 28533087 |
| molecular characteristics, biofilm-forming abilities, and quorum sensing molecules in vibrio parahaemolyticus strains isolated from marine and clinical environments in korea. | vibrio parahaemolyticus is an inhabitant of marine and estuarine environments and causes seafood-borne gastroenteritis in humans. in this study, an ultrafast labchip real-time pcr assay was evaluated for rapid detection and quantification of pathogenic v. parahaemolyticus isolates. escherichia coli and vibrio harveyi were used as negative controls. twenty-six tdh-positive, biofilm-producing v. parahaemolyticus isolates were analyzed by repetitive extragenic palindromic-polymerase chain reaction ... | 2017 | 28452235 |
| application of electrolysed oxidising water as a sanitiser to extend the shelf-life of seafood products: a review. | electrolysed oxidising water (e.o. water) is produced by electrolysis of sodium chloride to yield primarily chlorine based oxidising products. at neutral ph this results in hypochlorous acid in the un-protonated form which has the greatest oxidising potential and ability to penetrate microbial cell walls to disrupt the cell membranes. e.o. water has been shown to be an effective method to reduce microbial contamination on food processing surfaces. the efficacy of e.o. water against pathogenic ba ... | 2017 | 28416883 |
| groel is a suitable genetic marker for detecting vibrio parahaemolyticus by loop-mediated isothermal amplification assay. | a groel gene-based loop-mediated isothermal amplification (lamp) assay was developed to detect vibrio parahaemolyticus in contaminated seafood and water. the assay was optimized and conducted at 63°c for 40 min using bacillus stearothermophilus (bst) dna polymerase, large fragment. amplification was analysed via multiple detection methods, including opacity, formation of white precipitate, dna intercalating dyes (ethidium bromide and sybr green i), metal ion-binding indicator dye, calcein, and 2 ... | 2017 | 28585379 |
| purification and antibacterial mechanism of fish-borne bacteriocin and its application in shrimp (penaeus vannamei) for inhibiting vibrio parahaemolyticus. | vibrio parahaemolyticus: is recognized as the main cause of gastroenteritis associated with consumption of seafood. bacteriocin-producing lactobacillus plantarum fgc-12 isolated from golden carp intestine had strong antibacterial activity toward v. parahaemolyticus. the fish-borne bacteriocin was purified by a three-step procedure consisting of ethyl acetate extraction, gel filtration chromatography and high performance liquid chromatography. its molecular weight was estimated at 4.1 kda using s ... | 2017 | 28702797 |
| molecular identification of disk abalone (haliotis discus discus) tetraspanin 33 and cd63: insights into potent players in the disk abalone host defense system. | tetraspanins are a superfamily of transmembrane proteins involved in a diverse range of physiological processes including differentiation, adhesion, signal transduction, cell motility, and immune responses. in the present study, two tetraspanins, cd63 and tetraspanin 33 (tspan33) from disk abalone (abcd63 and abtspan33), were identified and characterized at the molecular level. the coding sequences for abcd63 and abtspan33 encoded polypeptides of 234 and 290 amino acids (aa) with predicted molec ... | 2017 | 28823981 |
| a cysteine protease (cathepsin z) from disk abalone, haliotis discus discus: genomic characterization and transcriptional profiling during bacterial infections. | cathepsin z (ctsz) is lysosomal cysteine protease of the papain superfamily. it participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. hence, ctsz is also acknowledged as an acute-phase protein in host immunity. in this study, we sought to identify the ctsz homolog from disk abalone (abctsz) and characterize it at the molecular, genomic, and transcrip ... | 2017 | 28687334 |
| new chlorinated xanthone and anthraquinone produced by a mangrove-derived fungus penicillium citrinum hl-5126. | two new chlorinated metabolites 4-chloro-1-hydroxy-3-methoxy-6-methyl-8-methoxycarbonyl-xanthen-9-one (1) and 2'-acetoxy-7-chlorocitreorosein (2), together with three known compounds (3-5), were obtained from the etoac extract of the endophytic fungus penicillium citrinum hl-5126 isolated from the mangrove bruguiera sexangula var. rhynchopetala collected in the south china sea. their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. all compounds were evalu ... | 2017 | 28465625 |
| antibacterial activities and preservative effect of chitosan oligosaccharide maillard reaction products on penaeus vannamei. | the purpose of this study was to understand the influence of maillard reaction (mr) on bioactivity of chitosan oligosaccharide (cos). the antibacterial activity against pseudomonas aeruginosa and vibrio parahaemolyticus and preservative effect of two kinds of cos mr products (mrps) on penaeus vannamei were evaluated. the structures of cg (mrps of cos and glucose) and cm (mrps of cos and maltose) were characterized by ft-ir and their molecular weights were measured by gel permeation chromatograph ... | 2017 | 28732733 |
| a c1qdc protein (hcc1qdc6) with three tandem c1q domains is involved in immune response of triangle-shell pearl mussel (hyriopsis cumingii). | c1q-domain-containing (c1qdc) proteins are a family of proteins with a globular c1q (gc1q) domain and participate in several immune responses. in this study, a c1qdc gene was identified from the triangle-shell pearl mussel hyriopsis cumingii (designated as hcc1qdc6). this gene has a full-length cdna of 1782 bp and an open reading frame of 1,335 bp that encodes a 444-amino acid polypeptide containing three gc1q domains. hcc1qdc6 contains at least five exons and four introns. the mrna transcripts ... | 2017 | 28785225 |
| microencapsulation of essential thyme oil by spray drying and its antimicrobial evaluation against vibrio alginolyticus and vibrio parahaemolyticus. | the aims of this research were first, to evaluate the antibacterial potential of commercial thyme essential oil against v. alginolyticus and v. parahaemolyticys and second, using the spray drying technique to produce microcapsules. chemical compounds of thyme oil and microcapsules were identified and quantified being thymol the chemical component present at the highest concentration. oil-in-water (o/w) emulsions were prepared and the microcapsules were obtained with a spray dryer using maltodext ... | 2017 | 28832836 |
| occurrence of vibrio species, beta-lactam resistant vibrio species, and indicator bacteria in ballast and port waters of a tropical harbor. | ballast water discharges are potential sources for the spread of invasive and pathogenic aquatic organisms. ballast waters from six ships docked in the port of singapore were tested to determine if indictor organisms fell within proposed standards for ballast water discharge according to regulation d-2 of the ballast water management convention (bwmc) guidelines. vibrio species were cultured on media supplemented with beta-lactam antibiotics to determine the presence of antibiotic resistant vibr ... | 2017 | 28822933 |
| molecular cloning, characterization and functional analysis of a putative mitogen-activated protein kinase kinase kinase 4 (mekk4) from blood clam tegillarca granosa. | the mitogen-activated protein kinase (mapk) cascades stand for one of the most important signaling mechanisms in response to environmental stimuli. in the present study, we cloned and identified for the first time the full-length cdna of mapk kinase kinase 4 (tgmekk4) from blood clam tegillarca granosa using rapid amplification of cdna ends method. the full-length cdna of tgmekk4 was of 1605 bp in length, encoding a polypeptide of 364 amino acids with a predicted molecular mass of 41.22 kda and ... | 2017 | 28476674 |
| environmental vibrios represent a source of antagonistic compounds that inhibit pathogenic vibrio cholerae and vibrio parahaemolyticus strains. | with the overuse of antibiotics, many pathogens including vibrio cholerae and vibrio parahaemolyticus have evolved multidrug resistance making treatment more difficult. while understanding the mechanisms that underlie pathogenesis is crucial, knowledge of bacterial interactions of v. cholerae and v. parahaemolyticus could provide insight to their susceptibility outside of the human host. based on previous work showing competition among environmental strains, we predict that marine-derived bacter ... | 2017 | 28857444 |
| effects of desiccation practices of cultured atlantic oysters (crassostrea virginica) on vibrio spp. in portersville bay, alabama, usa. | the expansion of off-bottom aquaculture to the gulf of mexico has raised public health concerns for human health officials. high temperatures in the gulf of mexico are associated with high levels of vibrio parahaemolyticus and vibrio vulnificus. routine desiccation practices associated with off-bottom aquaculture expose oysters to ambient air, allowing vibrio spp. to proliferate in the closed oyster. currently, there is limited research on the length of time needed for vibrio spp. levels in desi ... | 2017 | 28696147 |
| a double-quadratic model for predicting vibrio species in water environments of japan. | vibrio spp. are natural inhabitants of marine and estuarine environments. vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus are the major infectious agents for humans. their densities are affected by environmental factors such as water temperature and salinity. the detailed contribution of each factor still remains to be elucidated. here we conducted multi-coastal study in a 21-month period to examine relationships between environmental factors and v. cholerae, v. parahaemolyticus ... | 2017 | 28646477 |
| genome analysis of three novel lytic vibrio coralliilyticus phages isolated from seawater, okinawa, japan. | three novel vibrio phages were isolated from seawater in okinawa. the vibrio phage ryc infected vibrio coralliilyticus swa 07, while vibrio phages ckb-s1 and ckb-s2 infected the coral pathogen v. coralliilyticus p1 (lmg 23696). the vibrio phages ckb-s1 and ckb-s2 displayed head-tail structures whereas the vibrio phage ryc showed a tailless non-enveloped capsid. all these vibrio phages contained linear and double-stranded dna. the whole genome sequencing revealed that vibrio phage ryc has a large ... | 2017 | 28689690 |
| antagonistic activity of thymus vulgaris extracts against vibrio species isolated from seafoods. | this study examined the antibacterial activity of thymus vulgaris on multiple antibiotic resistant (mar) vibrio parahaemolyticus and vibrio fluvialis isolated from shrimps. the ethanol extract of t. vulgaris antibacterial properties was assessed using the agar diffusion method. survival of test organisms in shrimp meat using different concentrations of t. vulgaris was done using standard method. the quantitative and qualitative phytochemical tests of t. vulgaris extract were determined. the etha ... | 2017 | 28416870 |
| environmental determinants of vibrio parahaemolyticus in the chesapeake bay. | vibrio parahaemolyticus naturally-occurs in brackish and marine waters and is one of the leading causes of seafood-borne illness. previous work studying the ecology of v. parahaemolyticus is often limited in geographic extent and lacking a full range of environmental measures. this study used a unique, large dataset of surface water samples in the chesapeake bay (n=1,385) collected from 148 monitoring stations from 2007 to 2010. water was analyzed for over 20 environmental parameters with additi ... | 2017 | 28842541 |
| variation of genomic islands and flanking fragments in vibrio parahaemolyticus isolates from environmental and clinical sources in taiwan. | vibrio parahaemolyticus is a halophilic foodborne pathogenic bacterium that causes gastroenteritis; it has become an issue of global concern since the emergence and spread of pandemic o3:k6 strains. this study evaluated the role of vibrio pathogenicity island (vpai)-associated fragments in the genetic variation and grouping of this pathogen. distribution of some vpai fragments and flanking fragments (vpai-1, vpai-4, vpai-5, vpai-6 and vpai-7) was determined in a total of 53 v. parahaemolyticus i ... | 2017 | 28841447 |