Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| infectious disease exposures and outbreaks at a south african neonatal unit with review of neonatal outbreak epidemiology in africa. | hospitalized neonates are vulnerable to infection, with pathogen exposures occurring in utero, intrapartum, and postnatally. african neonatal units are at high risk of outbreaks owing to overcrowding, understaffing, and shared equipment. | 2017 | 28161461 |
| antimicrobial activity, ame resistance, and a-site binding studies of anthraquinone-neomycin conjugates. | the antibacterial effects of aminoglycosides are based on their association with the a-site of bacterial rrna and interference with the translational process in the bacterial cell, causing cell death. the clinical use of aminoglycosides is complicated by resistance and side effects, some of which arise from their interactions with the human mitochondrial 12s rrna and its deafness-associated mutations, c1494u and a1555g. we report a rapid assay that allows screening of aminoglycoside compounds to ... | 2017 | 28103015 |
| manual resuscitators in successive use in the same patient: reservoir of multi- and extensively resistant bacteria. | to determine the bacterial load on manual resuscitators (mrs), swab samples of mr connectors from a brazilian intensive care unit were collected at time zero, and after 24 and 48h use in the same patient. from 20 mrs, a total of 54 bacteria were isolated: six at t=0, 17 at t=24, and 31 at t=48. staphylococcus aureus, acinetobacter baumannii, serratia marcescens, and pseudomonas aeruginosa were the predominant isolates, and multidrug resistance was widespread in all species. greater awareness of ... | 2017 | 28012582 |
| bacteriological assessment of pneumonia caused by gram-negative bacteria in patients hospitalized in intensive care unit. | the article presents the results of 11-year study (2005-2015) of gram-negative bacteria responsible for pneumonia in 2033 mechanically ventilated patients hospitalized in intensive care unit. of 8796 biological samples, consisting mainly of bronchial aspirate (97.9 %), 2056 bacterial strains were isolated and subjected to identification. vitek 2 was used to determine drug susceptibility (classified according to the eucast criteria). esbl, mbl and kpc-producing strains were identified by means of ... | 2017 | 27739023 |
| incm plasmid r1215 is the source of chromosomally located regions containing multiple antibiotic resistance genes in the globally disseminated acinetobacter baumannii gc1 and gc2 clones. | clear similarities between antibiotic resistance islands in the chromosomes of extensively antibiotic-resistant isolates from the two dominant, globally distributed acinetobacter baumannii clones, gc1 and gc2, suggest a common origin. a close relative of the likely progenitor of both of these regions was found in r1215, a conjugative incm plasmid from a serratia marcescens strain isolated prior to 1980. the 37.8-kb resistance region in r1215 lies within the mucb gene and includes aacc1, aada1, a ... | 2017 | 27303751 |
| bacterial coaggregation among the most commonly isolated bacteria from contact lens cases. | to examine the coaggregation and cohesion between the commonly isolated bacteria from contact lens cases. | 2017 | 28061510 |
| coexistence of free-living amoebae and bacteria in selected south african hospital water distribution systems. | pathogenic free-living amoebae (fla), such as naegleria fowleri, balamuthia mandrillaris and acanthamoeba species isolated from aquatic environments have been implicated in central nervous system, eye and skin human infections. they also allow the survival, growth and transmission of bacteria such as legionella, mycobacteria and vibrio species in water systems. the purpose of this study was to investigate the co-occurrence of potentially pathogenic fla and their associated bacteria in hospital w ... | 2017 | 27730363 |
| characterization of batrachochytrium dendrobatidis inhibiting bacteria from amphibian populations in costa rica. | global amphibian declines and extinction events are occurring at an unprecedented rate. while several factors are responsible for declines and extinction, the fungal pathogen batrachochytrium dendrobatidis (bd) has been cited as a major constituent in these events. while the effects of this chytrid fungus have been shown to cause broad scale population declines and extinctions, certain individuals and relict populations have shown resistance. this resistance has been attributed in part to the cu ... | 2017 | 28293222 |
| the risk of microbial contamination in multiple-dose preservative-free ophthalmic preparations. | multiple-dose ophthalmic preparations that do not contain preservatives carry high risks of microbial contamination. however, there are various types of hospital preparations, with different physicochemical properties. in the present study, we evaluated the association between physicochemical properties and microbial contamination in ophthalmic preparations. the investigated hospital preparations included ophthalmic preparations of physiological saline, 0.2% fluconazole, 0.5% vancomycin hydrochl ... | 2017 | 28154258 |
| antimicrobial activity of prodigiosin is attributable to plasma-membrane damage. | the bacterial pigment prodigiosin has various biological activities; it is, for instance, an effective antimicrobial. here, we investigate the primary site targeted by prodigiosin, using the cells of microbial pathogens of humans as model systems: candida albicans, escherichia coli, staphylococcus aureus. inhibitory concentrations of prodigiosin; leakage of intracellular k(+) ions, amino acids, proteins and sugars; impacts on activities of proteases, catalases and oxidases; and changes in surfac ... | 2017 | 27353356 |
| chemical characterization and evaluation of antibacterial, antifungal, antimycobacterial, and cytotoxic activities of talinum paniculatum. | in this study, the bioactivity of talinum paniculatum was evaluated, a plant widely used in folk medicine. the extract from the t. paniculatum leaves (le) was obtained by percolation with ethanol-water and then subjecting it to liquid-liquid partitions, yielding hexane (hx), ethyl acetate (etoac), butanol (buoh), and aqueous (aq) fractions. screening for antimicrobial activity of the le and its fractions was evaluated in vitro through broth microdilution method, against thirteen pathogenic and n ... | 2017 | 26603226 |
| neonatal bacterial conjunctivitis in korea in the 21st century. | to investigate the microbial cause and antibiotic susceptibility of neonatal bacterial conjunctivitis in korean patients in the 21st century. | 2017 | 28002109 |
| comparative genomic analysis reveals organization, function and evolution of ars genes in pantoea spp. | numerous genes are involved in various strategies to resist toxic arsenic (as). however, the as resistance strategy in genus pantoea is poorly understood. in this study, a comparative genome analysis of 23 pantoea genomes was conducted. two vertical genetic arsc-like genes without any contribution to as resistance were found to exist in the 23 pantoea strains. besides the two arsc-like genes, as resistance gene clusters arsrbc or arsrbch were found in 15 pantoea genomes. these ars clusters were ... | 2017 | 28377759 |
| impact of revised broad-spectrum cephalosporin clinical and laboratory standards institute breakpoints on susceptibility in enterobacteriaceae producing ampc β-lactamase. | we evaluated the impact of revised clinical and laboratory standards institute (clsi) breakpoints for broad-spectrum cephalosporins (bscs) on the susceptibilities of 1,742 isolates of enterobacter species, serratia marcescens, citrobacter freundii, and morganella morganii. the 2011 clsi criteria for cefotaxime and ceftazidime reduced the rates of susceptibility by 2.9% and 5.9%, respectively. the 2014 clsi criteria for cefepime reduced the rate of susceptibility by 13.9%, and categorized 11.8% i ... | 2017 | 28271652 |
| carbonyl cyanide m-chlorophenylhydrazine (cccp) reverses resistance to colistin, but not to carbapenems and tigecycline in multidrug-resistant enterobacteriaceae. | background: carbapenems (car), colistin (cst), and tigecycline (tgc) alone or in combination therapy has become the last-resort antibiotics for treating infections caused by multidrug resistant (mdr) bacteria. however, resistance to these reserve antibiotics are increasingly being reported worldwide. hence, the quest to find other agents that will synergistically restore the efficacy of these antibiotics have increased. methods: sixty-three clinical enterobacteriaceae isolates comprising of kleb ... | 2017 | 28261184 |
| influence of tnf-α blockers on the oral prevalence of opportunistic microorganisms in ankylosing spondylitis patients. | to compare the oral prevalence and antimicrobial susceptibility of candida spp., staphylococci, enterobacteriaceae, and pseudomonas spp. from ankylosing spondylitis (as) patients receiving conventional and anti-tnf-α therapy. | 2017 | 22935381 |
| bacteria associated with piezodorus guildinii (hemiptera: pentatomidae), with special reference to those transmitted by feeding. | the redbanded stink bug, piezodorus guildinii (westwood) (hemiptera: heteroptera: pentatomidae), is a rapidly growing pest damaging southern us agriculture. pentatomid stink bugs are known to vector bacterial, fungal, and viral plant diseases. however, bacteria associated with redbanded stink bugs and their vector potential have not yet been assessed. in this study, we 1) cultured and identified bacteria transmitted by feeding of redbanded stink bug and 2) described bacteria from guts of redband ... | 2017 | 28025221 |
| searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds. | the aims of this study were to design, synthesize and to evaluate 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against gram-negative and gram-positive bacterial strains, including methicillin-resistant staphylococcus aureus (mrsa) and its biofilm, to probe for potential lead structures. | 2017 | 27930849 |
| high carbapenem resistance in clinical gram-negative pathogens isolated in egypt. | the emergence and spread of carbapenem-resistant gram-negative bacteria poses a serious threat to human health worldwide. currently, little is known about the molecular mechanisms underlying carbapenem resistance and their prevalence among gram-negative bacteria in egypt. in this study, we analyzed carbapenemase production in gram-negative bacteria isolated from hospitalized patients in egypt in 2014. all isolates were subjected to phenotypic and genotypic susceptibility testing for carbapenem r ... | 2017 | 28191865 |
| effect of intermittent aeration on microbial diversity in an intermittently aerated ifas reactor treating municipal wastewater: a field study. | in the present study, the effect of three intermittent aeration (ia) cycles on treatment performance and microbial diversity was investigated in an integrated fixed film activated sludge (ifas) reactor treating municipal wastewater. the results showed that ia strategies were able to achieve efficient removal of organics and nitrogen ranging between 90 and 95% and 70 and 80%, respectively, however the phosphorus removal was found to be inversely proportional to the duration of aeration off time i ... | 2017 | 28095182 |
| tailoring shape and size of biogenic silver nanoparticles to enhance antimicrobial efficacy against mdr bacteria. | spherical, rectangular, penta, and hexagonal silver nanoparticles of different dimensions were biosynthesized in an eco-friendly manner by biocontrol agent, trichoderma viride by manipulating physical parameters, ph, temperature, and reaction time. the particles were characterized by uv-vis spectroscopy; dynamic light scattering (dls), transmission electron microscopy (tem) and fourier transform infra-red spectroscopy (ftir). shape and size dependent antimicrobial activity of nanoparticles again ... | 2017 | 27889528 |
| characterization of two novel bacterial type a exo-chitobiose hydrolases having c-terminal 5/12-type carbohydrate-binding modules. | type a chitinases (ec 3.2.1.14), gh family 18, attack chitin ((1 → 4)-2-acetamido-2-deoxy-β-d-glucan) and chito-oligosaccharides from the reducing end to catalyze release of chitobiose (n,n'-diacetylchitobiose) via hydrolytic cleavage of n-acetyl-β-d-glucosaminide (1 → 4)-β-linkages and are thus "exo-chitobiose hydrolases." in this study, the chitinase type a from serratia marcescens (smachia) was used as a template for identifying two novel exo-chitobiose hydrolase type a enzymes, fbalchi18a an ... | 2017 | 28280871 |
| corneal ring infiltrates caused by serratia marcescens in a patient with human immunodeficiency virus. | to describe corneal ring infiltrates caused by serratia marcescens in a patient with human immunodeficiency virus (hiv-1) who wore contact lenses. | 2017 | 27721784 |
| serratia marcescens, morganella morganii, klebsiella oxytoca related peritonitis attacks in a patient on automated peritoneal dialysis: a case report. | 2017 | 28256286 | |
| the preparation and characterization of chitin and chitosan under large-scale submerged fermentation level using shrimp by-products as substrate. | the crustacean shells of crabs and shrimps produces quantities of by-products, leading to seriously environmental pollution and human health problems during industrial processing, yet they turned into high-value useful products, such as chitin and chitosan. to prepare them under large-scale submerged fermentation level, shrimp shell powders (ssps) was fermented by successive three-step fermentation of serratia marcescens b742, lactobacillus plantarum atcc 8014 and rhizopus japonicus m193 to extr ... | 2017 | 27965127 |
| bombyx mori serpin6 regulates prophenoloxidase activity and the expression of antimicrobial proteins. | serpins are a family of serine protease inhibitors that are found widely in insects. they play an important role in insect physiological responses, such as innate immunity and development. in this study, we obtained the bombyx mori serpin6 (bmserpin6) sequence from national center for biotechnology information (ncbi) and the silkworm genome database (silkdb). reverse transcription pcr (rt-pcr) results showed that bmserpin6 was expressed highly in hemocytes, the midgut, and the fat body. after ch ... | 2017 | 28188867 |
| umbilical infections in foals: microbiological investigation and management. | this study aims to investigate the bacteria involved in equine omphalitis and their susceptibility to antimicrobial drugs, and consequently to provide guidelines concerning the most suitable treatment protocol in accordance with the clinical, ultrasound and laboratory findings. forty foals aged between one and 30 days were evaluated in the course of this investigation. an ultrasound examination of all umbilical remnants was carried out carefully in all foals; umbilical swabs were collected for b ... | 2017 | 28314783 |
| long-chain 4-aminoquinolines as quorum sensing inhibitors in serratia marcescens and pseudomonas aeruginosa. | antibiotic resistance has become a serious global threat to public health; therefore, improved strategies and structurally novel antimicrobials are urgently needed to combat infectious diseases. here we report a new type of highly potent 4-aminoquinoline derivatives as quorum sensing inhibitors in serratia marcescens and pseudomonas aeruginosa, exhibiting weak bactericidal activities (minimum inhibitory concentration (mic) > 400 μm). through detailed structure-activity study, we have identified ... | 2017 | 28350449 |
| selenoprotein t is required for pathogenic bacteria avoidance in caenorhabditis elegans. | selenoprotein t (selenot) is an endoplasmatic reticulum (er)-associated redoxin that contains the amino acid selenocysteine (sec, u) within a cxxu motif within a thioredoxin-like fold. its precise function in multicellular organisms is not completely understood although it has been shown in mammals to be involved in ca(2+) homeostasis, antioxidant and neuroendocrine functions. here, we use the model organism c. elegans to address selenot function in a whole organism throughout its life cycle. c. ... | 2017 | 28347729 |
| oral polyphosphate suppresses bacterial collagenase production and prevents anastomotic leak due to serratia marcescens and pseudomonas aeruginosa. | the objective of this study was to determine the effect of polyphosphate on intestinal bacterial collagenase production and anastomotic leak in mice undergoing colon surgery. | 2017 | 28166091 |
| organoselenium polymer inhibits biofilm formation in polypropylene contact lens case material. | contact lens-acquired bacterial infections are a serious problem. of the reported cases, inadequate cleaning of the lens case was the most common cause of lens contamination. organoselenium has been shown to inhibit bacterial attachment to different polymer materials. this study evaluates the ability of an organoselenium monomer, incorporated into the polymer of a polypropylene contact lens case coupon, to block the formation of biofilms in a lens case. | 2017 | 26974534 |
| pore-forming toxin-mediated ion dysregulation leads to death receptor-independent necroptosis of lung epithelial cells during bacterial pneumonia. | we report that pore-forming toxins (pfts) induce respiratory epithelial cell necroptosis independently of death receptor signaling during bacterial pneumonia. instead, necroptosis was activated as a result of ion dysregulation arising from membrane permeabilization. pft-induced necroptosis required rip1, rip3 and mlkl, and could be induced in the absence or inhibition of tnfr1, tnfr2 and tlr4 signaling. we detected activated mlkl in the lungs from mice and nonhuman primates experiencing serratia ... | 2017 | 28387756 |
| slpe is a calcium-dependent cytotoxic metalloprotease associated with clinical isolates of serratia marcescens. | serralysin-like proteases are found in a wide variety of bacteria. these metalloproteases are frequently implicated in virulence and are members of the widely conserved rtx-toxin family. we identified a serralysin-like protease in the genome of a clinical isolate of serratia marcescens that is highly similar to the canonical serralysin protein, prts. this gene was named serralysin-like protease e, slpe, and was found in the majority (67%) of tested clinical isolates, but was absent from most tes ... | 2017 | 28366837 |
| fast purification method of functional lpmos from streptomyces ambofaciens by affinity adsorption. | a simple purification method by affinity adsorption was developed to obtain functional lytic polysaccharide monooxygenases (lpmos). the system allows the successful purification to homogeneity of the most characterized bacterial lpmo, cbp21 from serratia marcescens, and two lpmos from streptomyces ambofaciens, which have not been previously characterized. the first of these new lpmos, named samlpmo10b is a small enzyme (15 kda) belonging to family 10 of auxiliary activities (aa10), showing activ ... | 2017 | 28366436 |
| thermodynamics of tunnel formation upon substrate binding in a processive glycoside hydrolase. | glycoside hydrolases (ghs) catalyze the hydrolysis of glycosidic bonds and are key enzymes in carbohydrate metabolism. efficient degradation of recalcitrant polysaccharides such as chitin and cellulose is accomplished due to synergistic enzyme cocktails consisting of accessory enzymes and mixtures of ghs with different modes of action and active site topologies. the substrate binding sites of chitinases and cellulases often have surface exposed aromatic amino acids and a tunnel or cleft topology ... | 2017 | 28359644 |
| killing of serratia marcescens biofilms with chloramphenicol. | serratia marcescens is a gram-negative bacterium with proven resistance to multiple antibiotics and causative of catheter-associated infections. bacterial colonization of catheters mainly involves the formation of biofilm. the objectives of this study were to explore the susceptibility of s. marcescens biofilms to high doses of common antibiotics and non-antimicrobial agents. biofilms formed by a clinical isolate of s. marcescens were treated with ceftriaxone, kanamycin, gentamicin, and chloramp ... | 2017 | 28356113 |
| site-specific variation in gene expression from symbiodinium spp. associated with offshore and inshore porites astreoides in the lower florida keys is lost with bleaching and disease stress. | scleractinian coral are experiencing unprecedented rates of mortality due to increases in sea surface temperatures in response to global climate change. some coral species however, survive high temperature events due to a reduced susceptibility to bleaching. we investigated the relationship between bleaching susceptibility and expression of five metabolically related genes of symbiodinium spp. from the coral porites astreoides originating from an inshore and offshore reef in the florida keys. th ... | 2017 | 28355291 |
| polyclonal dissemination of kpc-2 in serratia marcescens, including a clone with epidemic behaviour in the nosocomial niche. | 2017 | 28336311 | |
| [serratia infections, should we think about primary immunodeficiencies?] | chronic granulomatous disease (cgd) is a primary immunodeficiency with an incidence of 1/200,000-250,000 live births. cgd affects mainly male patients, most of the mutations being x-linked, and autosomal recessive forms occur more frequently in communities with greater numbers of consanguineous marriages. cgd is characterized by sensitivity to recurrent and severe bacterial and fungal infections, with formation of granulomas due to the inability of phagocytes to generate reactive oxygen compound ... | 2017 | 28318195 |
| fulminant serratia marcescens panophthalmitis. | 2017 | 28317639 | |
| scleral buckle infection by serratia species. | we describe a rare case of scleral buckle (sb) infection with serratia species. a 48-year-old male with a history of retinal detachment repair with scleral buckling presented with redness, pain, and purulent discharge in the left eye for 4 days. conjunctival erosion with exposure of the sb and scleral thinning was noted. the sb was removed and sent for culture. blood and chocolate agar grew gram-negative rod-shaped bacillus identified as serratia marcescens. on the basis of the susceptibility te ... | 2017 | 28298863 |
| a novel expression system for lytic polysaccharide monooxygenases. | lytic polysaccharide monooxygenases (lpmos) are key enzymatic players of lignocellulosic biomass degradation processes. as such, they have been introduced in cellulolytic cocktails for more efficient and less expensive lignocellulose saccharification. the recombinant production of lpmos in bacteria for scientific investigations using vectors typically based on the t7 and lacuv5 promoters has been hampered by low yields. reasons for this have been catabolite repression when producing the proteins ... | 2017 | 28291518 |
| chorioamnionitis caused by serratia marcescens in a healthy pregnant woman with preterm premature rupture of membranes: a rare case report and review of the literature. | the incidence of chorioamnionitis varies widely. the highest incidence is reported in preterm deliveries. among preterm deliveries, chorioamnionitis usually occurs after preterm premature rupture of membranes (pprom). to date, only five cases of chorioamnionitis due to serratia marcescens were reported. here we present a case of a pregnant woman with chorioamnionitis due to serratia marcescens who delivered a premature neonate at 28 weeks and four days of gestation. we also conducted a review of ... | 2017 | 28283205 |
| draft genome sequence of a multidrug-resistant nosocomial serratia marcescens strain that persisted in a hospital in kemerovo, russian federation. | serratia marcescens is a frequent cause of health care-associated infections and has led to multiple outbreaks. here, we report the draft genome of a multidrug-resistant s. marcescens strain 189 which was isolated in 2012 as a predominant clone in a neonatal hospital in kemerovo. | 2017 | 28280035 |
| suppressor analysis of eepr mutant defects reveals coordinate regulation of secondary metabolites and serralysin biosynthesis by eepr and hexs. | the eepr transcription factor positively regulates secondary metabolites and tissue-damaging metalloproteases. to gain insight into mechanisms by which eepr regulates pigment and co-regulated factors, genetic suppressor analysis was performed. suppressor mutations that restored pigment to the non-pigmented ∆eepr mutant mapped to the hexs orf. mutation of hexs also restored haemolysis, swarming motility and protease production to the eepr mutant. hexs is a known direct and negative regulator of s ... | 2017 | 28270264 |
| crystal structure of mbp-pigg fusion protein and the essential function of pigg in the prodigiosin biosynthetic pathway in serratia marcescens fs14. | prodigiosin, a tripyrrole red pigment is synthesized by serratia and some other microbes through a bifurcated biosynthesis pathway; mbc (4-methoxy-2,2'-bipyrrole-5-carbaldehyde) and map (2-methyl-3-n-amyl-pyrrole) are synthesized separately and then condensed by pigc to form prodigiosin. pigi, pigg and piga have been shown to be involved in the first steps of mbc biosynthesis (proline incorporation). the crystal structure of pigg was resolved to elucidate its function and mechanism. pigg, an acy ... | 2017 | 28258005 |
| genome sequence of serratia marcescens msu97, a plant-associated bacterium that makes multiple antibiotics. | serratia marcescens msu97 was isolated from the guayana region of venezuela due to its ability to suppress plant-pathogenic oomycetes. here, we report the genome sequence of msu97, which produces various antibiotics, including the bacterial acetyl-coenzyme a (acetyl-coa) carboxylase inhibitor andrimid, the chlorinated macrolide oocydin a, and the red linear tripyrrole antibiotic prodigiosin. | 2017 | 28254993 |
| cultivable gut bacteria provide a pathway for adaptation of chrysolina herbacea to mentha aquatica volatiles. | a chemical cross-talk between plants and insects is required in order to achieve a successful co-adaptation. in response to herbivory, plants produce specific compounds, and feeding insects respond adequately7 to molecules produced by plants. here we show the role of the gut microbial community of the mint beetle chrysolina herbacea in the chemical cross-talk with mentha aquatica (or watermint). | 2017 | 28249605 |
| are genital ulcers always sexually transmitted? first report of scrotal ulcer caused by serratia marcescens infection. | 2017 | 28239854 | |
| scrotal ulcer due to serratia marcescens. | 2017 | 28196788 | |
| complete genome analysis of serratia marcescens rsc-14: a plant growth-promoting bacterium that alleviates cadmium stress in host plants. | serratia marcescens rsc-14 is a gram-negative bacterium that was previously isolated from the surface-sterilized roots of the cd-hyperaccumulator solanum nigrum. the strain stimulates plant growth and alleviates cd stress in host plants. to investigate the genetic basis for these traits, the complete genome of rsc-14 was obtained by single-molecule real-time sequencing. the genome of s. marcescens rsc-14 comprised a 5.12-mbp-long circular chromosome containing 4,593 predicted protein-coding gene ... | 2017 | 28187139 |
| lepidopteran species have a variety of defence strategies against bacterial infections. | the insect immune system has versatile ways of coping with microbial insults. currently, innate immune priming has been described in several invertebrates, and the first insights into its mechanistic basis have been described. here we studied infections with two different strains of serratia marcescens bacteria in two different lepidopteran hosts. the results reveal fundamental differences between the two hosts, a well-known model organism galleria mellonella and a non-model species arctia plant ... | 2017 | 28163013 |
| is there a role for serratia marcescens in male infertility: an experimental study? | establishment of a male balb/c mouse model to study the role of sperm impairing s. marcescens on mouse reproductive potential. the current study can add to use of reliable animal models to provide a noteworthy evidence for the microbial cause of infertility. | 2017 | 28161359 |
| serratia marcescens harboring sme-4 in brazil: a silent threat. | the intrinsic polymyxin resistance displayed by serratia marcescens makes the acquisition of carbapenemase encoding genes a worrisome event. this study report a sme-4-producing s. marcescens isolate causing septic shock in brazil. the insertion of novel resistance determinants and their consequent spread in our territory is noteworthy. | 2017 | 28159445 |
| phytochemistry and pharmacology of ferula persica boiss.: a review. | ferula persica, is the well-known species of the genus ferula in iran and has two varieties: persica and latisecta. they have both been extensively used in traditional medicine for a wide range of ailments. a great number of chemical compounds including sesquiterpene coumarins and polysulfides have been isolated from this plant. fresh plant materials, crude extracts and isolated components of f. persica have shown a wide spectrum of pharmacological properties including anti-pigmentation in serra ... | 2017 | 28133517 |
| genomic, proteomic and biochemical analysis of the chitinolytic machinery of serratia marcescens bjl200. | the chitinolytic machinery of serratia marcescens bjl200 has been studied in detail over the last couple of decades, however, the proteome secreted by this gram-negative bacterium during growth on chitin has not been studied in depth. in addition, the genome of this most studied chitinolytic serratia strain has until now, not been sequenced. we report a draft genome sequence for s. marcescens bjl200. using label-free quantification (lfq) proteomics and a recently developed plate-method for asses ... | 2017 | 28130068 |
| human chitotriosidase is an endo-processive enzyme. | human chitotriosidase (hcht) is involved in immune response to chitin-containing pathogens in humans. the enzyme is able to degrade chitooligosaccharides as well as crystalline chitin. the catalytic domain of hcht is connected to the carbohydrate binding module (cbm) through a flexible hinge region. in humans, two active isoforms of hcht are found-the full length enzyme and its truncated version lacking cbm and the hinge region. the active site architecture of hcht is reminiscent to that of the ... | 2017 | 28129403 |
| serratia marcescens producing sme carbapenemases: an emerging resistance problem in the uk? | 2017 | 28108680 | |
| draft genome sequences of pandrug-resistant serratia marcescens clinical isolates harboring blandm-1. | the draft genome sequences of two clonal, pandrug-resistant serratia marcescens clinical isolates were determined. the resistance phenotype was plasmid driven, as 14 of 17 resistance genes were present on large incfib(k), inchi2, and inca/c2 plasmids indicating a large pool of transmissible antibiotic resistance genes. | 2017 | 28104656 |
| wound and soft tissue infections of serratia marcescens in patients receiving wound care: a health care-associated outbreak. | we described a health care-associated serratia marcescens outbreak of wound and soft tissue infection lasting approximately 11 months at ankara university ibni sina hospital. after identification of s marcescens strains from the clinical and environmental samples, and their susceptibility testing to antimicrobial agents, pulsed-field gel electrophoresis (pfge) was performed to detect molecular epidemiologic relationships among these isolates. the strains which were isolated from the saline bottl ... | 2017 | 28063729 |
| elimination and molecular identification of endophytic bacterial contaminants during in vitro propagation of bambusa balcooa. | bambusa balcooa is an economically important, multipurpose bamboo species, decidedly used in construction industry. availability of natural bamboo is depleting very rapidly due to accelerated deforestation and its unrestrained use. the large number and timely supply of saplings are the need of the hour for the restoration of bamboo stands. micropropagation, being the potent alternative for season independent rapid regeneration, is restricted in bamboo because of endophytic contamination. an in v ... | 2017 | 28063101 |
| protracted regional dissemination of gim-1-producing serratia marcescens in western germany. | the metallo-beta-lactamase gim-1 has been found in various bacterial host species nearly exclusively in western germany. however, not much is known about the epidemiology of gim-1-positive serratia marcescens here we report on a surprisingly protracted regional dissemination. in-hospital transmission was investigated by using conventional epidemiological tools to identify spatiotemporal links. strain typing was performed using pulsed-field gel electrophoresis (pfge) and whole-genome sequencing ( ... | 2017 | 27956426 |
| draft genome sequence of a ges-5-producing serratia marcescens isolated in southern brazil. | serratia marcescens is a gram-negative rod intrinsically resistant to polymyxins and usually associated with wound, respiratory and urinary tract infections. the whole genome of the first ges-5-producing s. marcescens isolated from a brazilian patient was sequenced using ion torrent pgm system. besides blages-5, we were able to identify genes encoding for other β-lactamases, for aminoglycoside modifying enzymes and for an efflux pump to tetracyclines. | 2017 | 27932081 |
| a pore-forming toxin enables serratia a nonlytic egress from host cells. | several pathogens co-opt host intracellular compartments to survive and replicate, and they thereafter disperse progeny to prosper in a new niche. little is known about strategies displayed by serratia marcescens to defeat immune responses and disseminate afterwards. upon invasion of nonphagocytic cells, serratia multiplies within autophagosome-like vacuoles. these serratia-containing vacuoles (secv) circumvent progression into acidic/degradative compartments, avoiding elimination. in this work, ... | 2017 | 27532510 |
| association of plant growth-promoting serratia spp. with the root nodules of chickpea. | serratia species-affiliated dna sequences have recently been discovered in the root nodules of two chickpea cultivars; however, little is known about their potential influence on chickpea plant growth. all serratia-affiliated sequences (1136) could be grouped into two clusters at 98% dna similarity. the major cluster, represented by 96% of sequences, was closely associated with serratia marcescens sequences from genbank. in the current study, we isolated two serratia strains, 5d and rtl100, from ... | 2017 | 27117242 |
| computational identification of potent inhibitors for streptomycin 3″-adenylyltransferase of serratia marcescens. | serratia marcescens is an opportunistic pathogen responsible for the respiratory and urinary tract infections in humans. the antibiotic resistance mechanism of s. marcescens is mediated through aminoglycoside modification enzyme that transfer adenyl group from substrate to antibiotic through regiospecific transfers for the inactivation of antibiotics. streptomycin 3(″)-adenylyltransferase acts on the 3' position of the antibiotic and considered as a novel drug target to overcome bacterial antibi ... | 2017 | 27993702 |
| characterization of putative virulence factors of serratia marcescens strain sen for pathogenesis in spodoptera litura. | two serratia marcescens strains, sen and icc-4, isolated from diseased insect cadavers were observed to differ considerably in their virulence towards spodoptera litura. the present study was aimed to characterize the possible virulence factors present in the virulent serratia marcescens strain sen. both the s. marcescens strains were evaluated for the presence of various lytic enzymes such as chitinase, lipase, protease and phospholipase. the virulent s. marcescens strain sen was observed to po ... | 2017 | 27993620 |
| extraction and encapsulation of prodigiosin in chitosan microspheres for targeted drug delivery. | the encapsulation of drugs in polymeric materials has brought opportunities to the targeted delivery of chemotherapeutic agents. these polymeric delivery systems are capable of maximizing the therapeutic activity, as well as reducing the side effects of anti-cancer agents. prodigiosin, a secondary metabolite extracted from the bacteria, serratia marcescens, exhibits anti-cancer properties. prodigiosin-loaded chitosan microspheres were prepared via water-in-oil (w/o) emulsion technique, using glu ... | 2017 | 27987708 |
| serratia marcescens bacteraemia outbreak in haemodialysis patients with tunnelled catheters due to colonisation of antiseptic solution. experience at 4 hospitals. | the application of antiseptic solution for handling tunnelled catheters is recommended in patients undergoing haemodialysis. these routine antiseptic procedures in handling catheters are crucial to avoid complications. we report an outbreak of serratia marcescens (s. marcescens) bacteraemia in numerous haemodialysis units of the community of madrid. | 2017 | 27595511 |
| an antimicrobial protein of the riptortus pedestris salivary gland was cleaved by a virulence factor of serratia marcescens. | recently, our group demonstrated that the bean bug, riptortus pedestris, is a good experimental symbiosis model to study the molecular cross-talk between the host insect and the gut symbiont. the burkholderia symbiont is orally acquired by host nymphs from the environment in every generation. however, it is still unclear how riptortus specifically interacts with entomopathogens that are abundant in the environmental soil. in preliminary experiments, we observed that a potent entomopathogen, serr ... | 2017 | 27555079 |
| metastatic serratia endophthalmitis associated with extravasation injury in a preterm neonate. | the authors report a case of a preterm neonate who presented with lid edema, corneal edema, and an inflammatory membrane with whitish exudates in the pupillary area, suggestive of endophthalmitis. there was also a cutaneous ulcer with an eschar on the right wrist at the site of extravasation associated with previous intravenous catheter. cultures from the ulcer and vitreous samples both grew serratia marcescens with identical antibiotic sensitivity and resistance patterns. the ocular infection w ... | 2017 | 26622140 |
| kpc-producing serratia marcescens in a home-care patient from recife, brazil. | in this brief communication we describe the occurrence of a kpc-producing serratia marcescens isolate in a home-care patient from recife, brazil. the blakpc, blaspm, blaimp, blavim, blaoxa, blactx-m, blashv, blatem and blages genes were investigated by polymerase chain reaction (pcr) and dna sequencing. the isolate was positive for blakpc-2 and blatem-1 and was resistant to aztreonam, cefepime, cefotaxime, imipenem, meropenem, gentamicin, ciprofloxacin and cefazidime, and susceptible only to ami ... | 2017 | 26422164 |
| differential sensitivity to infections and antimicrobial peptide-mediated immune response in four silkworm strains with different geographical origin. | the domesticated silkworm bombyx mori has an innate immune system, whose main effectors are the antimicrobial peptides (amps). silkworm strains are commonly grouped into four geographical types (japanese, chinese, european and tropical) and are generally characterised by a variable susceptibility to infections. to clarify the genetic and molecular mechanisms on which the different responses to infections are based, we exposed one silkworm strain for each geographical area to oral infections with ... | 2017 | 28432358 |
| protein engineering and homologous expression of serratia marcescens lipase for efficient synthesis of a pharmaceutically relevant chiral epoxyester. | the lipase isolated from serratia marcescens (lipa) is a useful biocatalyst for kinetic resolution of a pharmaceutically relevant epoxyester, (±)-3-(4'-methoxyphenyl) glycidic acid methyl ester [(±)-mpgm], to afford optically pure (-)-mpgm, a key intermediate for the synthesis of diltiazem hydrochloride. two mutants, lipal315s and lipas271f, were identified from the combinatorial saturation mutation library of 14 amino acid residues lining the substrate-binding pocket. lipal315s, lipas271f, and ... | 2017 | 28766104 |
| biological activities and secondary metabolite screening of rumex hastatus extract through fourier transform infrared and raman spectroscopy. | human infectious diseases began by various pathogens including bacteria, fungi, viruses, parasites, and protozoans. these infectious agents are controlled by using synthetic drugs as well as natural sources. | 2017 | 28758575 |
| antimicrobial resistance patterns of gram-negative bacteria isolated from bloodstream infections in an iranian referral pediatric hospital: a six-year study. | bloodstream infections (bsis) are considered as a major cause of morbidity and mortality in children all over the world. the aim of this study was to describe the epidemiology and antimicrobial resistance patterns of gram-negative bacteria (gnb) isolated from bloodstream infections (bsis) in children admitted to the children medical center hospital (cmc), tehran, iran. | 2017 | 28729206 |
| quantitative determination of anti-bacterial activity during bacterial co-culture. | anti-bacterial activity assays are an important tool in the assessment of the ability of one bacterium to kill or inhibit the growth of another, for example during the study of the type vi secretion system (t6ss) and the anti-bacterial toxins it secretes. the method we describe here can detect the ability of a bacterial strain to kill or inhibit other bacterial cells in a contact-dependent manner when co-cultured on an agar surface. it is particularly useful since it enumerates the recovery of v ... | 2017 | 28667634 |
| host mating system and coevolutionary dynamics shape the evolution of parasite avoidance in caenorhabditis elegans host populations. | hosts exhibit a variety of defence mechanisms against parasites, including avoidance. both host-parasite coevolutionary dynamics and the host mating system can alter the evolutionary trajectories of populations. does the nature of host-parasite interactions and the host mating system affect the mechanisms that evolve to confer host defence? in a previous experimental evolution study, mixed mating and obligately outcrossing caenorhabditis elegans host populations adapted to either coevolving or s ... | 2017 | 28655368 |
| crystal structure of the sensor domain of baes from serratia marcescens fs14. | the sensor histidine kinases of two-component signal-transduction systems (tcss) are essential for bacteria to adapt to variable environmental conditions. the two-component regulatory system baes/r increases multidrug and metal resistance in salmonella and escherichia coli. in this study, we report the x-ray structure of the periplasmic sensor domain of baes from serratia marcescens fs14. the baes sensor domain (34-160) adopts a mixed α/β-fold containing a central four-stranded antiparallel β-sh ... | 2017 | 28544098 |
| imipenem-resistance in serratia marcescens is mediated by plasmid expression of kpc-2. | imipenem is a broad-spectrum carbapenem antibiotic with applications against severe bacterial infections. here, we describe the identification of imipenem-resistant serratia marcescens in our hospital and the role of plasmid-mediated kpc-2 expression in imipenem resistance. | 2017 | 28429335 |
| transfer potential of plasmids conferring extended-spectrum-cephalosporin resistance in escherichia coli from poultry. | escherichia coli strains resistant to extended-spectrum cephalosporins (esc) are widely distributed in norwegian broiler production, and the majority harbor transferable inck or inci1 plasmids carrying blacmy-2 persistent occurrence in broiler farms may occur through the survival of esc-resistant e. coli strains in the farm environment, or by transfer and maintenance of resistance plasmids within a population of environmental bacteria with high survival abilities. the aim of this study was to de ... | 2017 | 28411217 |
| amycolatopsis acidiphila sp. nov., a moderately acidophilic species isolated from coal mine soil. | little is known on members of the genus amycolatopsis inhabiting acidic habitats. in this study, a moderately acidophilic amycolatopsis strain, designated 2-5t, was isolated from coal mine soil, and subjected to a polyphasic taxonomic characterization. analysis based on 16s rrna gene sequences indicated that the strain was most closely related to the type strain of amycolatopsis bartoniae, sharing 99.30 % similarity, while similarity to all other amycolatopsis species was less than 97 %. the dna ... | 2017 | 28857027 |
| klebsiella pneumoniae carbapenemase-producing serratia marcescens outbreak in a university hospital. | 2017 | 28431850 | |
| neonicotinoid-induced pathogen susceptibility is mitigated by lactobacillus plantarum immune stimulation in a drosophila melanogaster model. | pesticides are used extensively in food production to maximize crop yields. however, neonicotinoid insecticides exert unintentional toxicity to honey bees (apis mellifera) that may partially be associated with massive population declines referred to as colony collapse disorder. we hypothesized that imidacloprid (common neonicotinoid; imi) exposure would make drosophila melanogaster (an insect model for the honey bee) more susceptible to bacterial pathogens, heat stress, and intestinal dysbiosis. ... | 2017 | 28578396 |
| the risk of cardiac device-related infection in bacteremic patients is species specific: results of a 12-year prospective cohort. | the species-specific risk of cardiac device-related infection (cdri) among bacteremic patients is incompletely understood. | 2017 | 28852678 |
| pseudomonas aeruginosa exla and serratia marcescens shla trigger cadherin cleavage by promoting calcium influx and adam10 activation. | pore-forming toxins are potent virulence factors secreted by a large array of bacteria. here, we deciphered the action of exla from pseudomonas aeruginosa and shla from serratia marcescens on host cell-cell junctions. exla and shla are two members of a unique family of pore-forming toxins secreted by a two-component secretion system. bacteria secreting either toxin induced an exla- or shla-dependent rapid cleavage of e-cadherin and ve-cadherin in epithelial and endothelial cells, respectively. c ... | 2017 | 28832671 |
| antimicrobial efficacy of contact lens care solutions against neutrophil-enhanced bacterial biofilms. | neutrophil-derived extracellular debris has been shown to accelerate bacterial biofilm formation on hydrogel and silicone hydrogel contact lens surfaces compared to lenses inoculated with bacteria alone. the purpose of this study was to evaluate the disinfection efficacy of four standard commercial contact lens cleaning regimens against neutrophil-enhanced bacterial biofilms formed on silicone hydrogel contact lenses. | 2017 | 28473944 |
| factors associated with infectious laryngitis: a retrospective review of 15 cases. | to identify the culturable microbes associated with infectious laryngitis and outline effective treatment strategies. | 2017 | 28397557 |
| new prodigiosin derivatives obtained by mutasynthesis in pseudomonas putida. | the deeply red-colored natural compound prodigiosin is a representative of the prodiginine alkaloid family, which possesses bioactivities as antimicrobial, antitumor, and antimalarial agents. various bacteria including the opportunistic human pathogen serratia marcescens and different members of the streptomycetaceae and pseudoalteromonadaceae produce prodiginines. in addition, these microbes generally accumulate many structurally related alkaloids making efficient prodiginine synthesis and puri ... | 2017 | 28505410 |
| a transcriptional regulatory mechanism finely tunes the firing of type vi secretion system in response to bacterial enemies. | the ability to detect and measure danger from an environmental signal is paramount for bacteria to respond accordingly, deploying strategies that halt or counteract potential cellular injury and maximize survival chances. type vi secretion systems (t6sss) are complex bacterial contractile nanomachines able to target toxic effectors into neighboring bacteria competing for the same colonization niche. previous studies support the concept that either t6sss are constitutively active or they fire eff ... | 2017 | 28830939 |
| a comparative study of the adhesion of biosynthesized gold and conjugated gold/prodigiosin nanoparticles to triple negative breast cancer cells. | this paper explores the adhesion of biosynthesized gold nanoparticles (aunps) and gold (au) nanoparticle/prodigiosin (pg) drug nanoparticles to breast cancer cells (mda-mb-231 cells). the aunps were synthesized in a record time (less than 30 s) from nauclea latifolia leaf extracts, while the pg was produced via bacterial synthesis with serratia marcescens sp. the size distributions and shapes of the resulting aunps were characterized using transmission electron microscopy (tem), while the result ... | 2017 | 28819929 |
| multi-fractal characterization of bacterial swimming dynamics: a case study on real and simulated serratia marcescens. | to add to the current state of knowledge about bacterial swimming dynamics, in this paper, we study the fractal swimming dynamics of populations of serratia marcescens bacteria both in vitro and in silico, while accounting for realistic conditions like volume exclusion, chemical interactions, obstacles and distribution of chemoattractant in the environment. while previous research has shown that bacterial motion is non-ergodic, we demonstrate that, besides the non-ergodicity, the bacterial swimm ... | 2017 | 28804259 |
| evolution of caenorhabditis elegans host defense under selection by the bacterial parasite serratia marcescens. | parasites can impose strong selection on hosts. in response, some host populations have adapted via the evolution of defenses that prevent or impede infection by parasites. however, host populations have also evolved life history shifts that maximize host fitness despite infection. outcrossing and self-fertilization can have contrasting effects on evolutionary trajectories of host populations. while selfing and outcrossing are known to affect the rate at which host populations adapt in response ... | 2017 | 28792961 |
| resistance phenotypes and susceptibility of contemporary serratia isolates in the university hospital of crete, greece. | to study changes in the susceptibility of serratia spp. in crete, greece (2010-2015). | 2017 | 28768448 |
| isolation of vanadium-resistance endophytic bacterium pre01 from pteris vittata in stone coal smelting district and characterization for potential use in phytoremediation. | this study investigates the v-resistant endophytic bacteria isolated from v-accumulator pteris vittata grown on stone coal smelting district. among all the ten isolates, the strain pre01 identified as serratia marcescens ss marcescens by biolog gen iii microplate™ was screened out by ranking first in terms of heavy metal resistance and plant growth promoting traits. the s. marcescens pre01 had strong v, cr and cd resistance especially for v up to 1500mg/l. in addition, it exhibited acc deaminase ... | 2017 | 28759788 |
| an unusual case of acute parotitis in a young adult. | acute bacterial parotitis is uncommon in young adults. infection with serratia marcescens is even rarer and usually found in hospitalized patients. this case report focuses on a young woman with acute bacterial parotitis caused by s. marcescens that required a longer-than-normal course of antibiotics. | 2017 | 28742741 |
| synthesis and characterization of nanoparticles conjugated tannase and using it for enhancement of antibacterial activity of tannase produced by serratia marcescens. | fourteen isolates of serratia marcescens were collected from patients suffering from septicemia. all theseisolates revealed different levels in tannase production. tannase was partially purified from serratia marcescens b9 by precipitation method at 70% saturation of ammonium sulfate. au, pt, sno2 and sio2 nanoparticles were prepared by laser ablation and examined by transmission electron microscopy (tem), x-ray diffraction pattern and uv-visible absorption spectroscopy. conjugation of sio2 nano ... | 2017 | 28735079 |
| inhibitory growth of oral squamous cell carcinoma cancer via bacterial prodigiosin. | chemotherapy drugs for oral cancers always cause side effects and adverse effects. currently natural sources and herbs are being searched for treated human oral squamous carcinoma cells (oscc) in an effort to alleviate the causations of agents in oral cancers chemotherapy. this study investigates the effect of prodigiosin (pg), an alkaloid and natural red pigment as a secondary metabolite of serratia marcescens, to inhibit human oral squamous carcinoma cell growth; thereby, developing a new drug ... | 2017 | 28714874 |
| tnseqdiff: identification of conditionally essential genes in transposon sequencing studies. | tn-seq is a high throughput technique for analysis of transposon mutant libraries to determine conditional essentiality of a gene under an experimental condition. a special feature of the tn-seq data is that multiple mutants in a gene provides independent evidence to prioritize that gene as being essential. the existing methods do not account for this feature or rely on a high-density transposon library. moreover, these methods are unable to accommodate complex designs. | 2017 | 28683752 |
| regulation of p53 and survivin by prodigiosin compound derived from serratia marcescens contribute to caspase-3-dependent apoptosis in acute lymphoblastic leukemia cells. | tumor suppressor p53 and proto-oncogene survivin are challenging targets for anticancer drugs in acute lymphoblastic leukemia (all) which are associated with chemoresistance. yet, no p53 and survivin-modulating drug with low toxicity and high efficacy has been approved for clinical application in all. consequently, the search for novel compounds which target p53 or survivin is needed to further advance all treatment. prodigiosin, a secondary metabolite of serratia marcescens induces apoptosis in ... | 2017 | 28681618 |