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[alpha 1 - antitrypsin, a modern glycoprotein]. 20164733188
[mirin dajo]. 20164874675
[new research on the inhibitory action of methyl-3 hydroxy-6 quinazoline dione 2-4 on the cytodierese of plant cells. duration of the cell cycle and mitosis]. 20164802674
nosocomial bacteremia due to kluyvera cryocrescens: case report and literature review.kluyvera cryocrescens infection has been considered rare; clinical features of k. cryocrescens bacteremia remain unclear because few reports have been published. we report a case of k. cryocrescens bacteremia in an adult male patient and review the literature. our case was one with nosocomial bacteremia in a patient with interstitial lung disease. the primary infection site was undetermined, although he had an indwelling peripheral intravenous catheter and a urinary catheter. piperacilin/tazobac ...201627051581
[achievements in urology in the ussr]. 20154592854
homologous scleral explant buckles in retinal detachment surgery. 20154594665
molecular characteristics of extended-spectrum cephalosporin-resistant enterobacteriaceae from humans in the community.to investigate the molecular characteristics of extended-spectrum cephalosporin (esc)-resistant enterobacteriaceae collected during a cross-sectional study examining the prevalence and risk factors for faecal carriage of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in humans living in areas with high or low broiler density.201526029910
structure mediation in substrate binding and post-translational processing of penicillin acylases: information from mutant structures of kluyvera citrophila penicillin g acylase.penicillin acylases are industrially important enzymes for the production of 6-apa, which is used extensively in the synthesis of secondary antibiotics. the enzyme translates into an inactive single chain precursor that subsequently gets processed by the removal of a spacer peptide connecting the chains of the mature active heterodimer. we have cloned the penicillin g acylase from kluyvera citrophila (kcpga) and prepared two mutants by site-directed mutagenesis. replacement of n-terminal serine ...201526243007
[musculus rectus labii and its significance for the lip mobility of chimpanzees (pan troglodytes)]. 20154451282
tuberous sclerosis and schizophrenia. 20154648445
the histaminase activity of ceruloplasmin. 20154630290
role of mental imagery in free recall of deaf, blind, and normal sunjects. 20154705238
education and the hospital: hospital office promotes health education. 20144119583
physiological stress and performance changes in response to beginning level orienteering. 20144068683
splenic syndrome at mountain altitudes in sickle cell trait. its occurrence in nonblack persons.six consecutive cases of splenic syndrome at mountain altitudes in persons with sickle cell trait are reported and the literature is reviewed. all six cases occurred in men who experienced the acute onset of severe left-upper-quadrant abdominal pain within 48 hours of arrival in colorado from lower altitudes. all six patients were phenotypically nonblack. three patients experienced their symptoms at moderate altitudes of 1,609 to 2,134 m (5,280 to 7,000 ft) above sea level. all recovered with me ...20143974118
electron microscopic structure of the "inclusion bodies" in pick's disease. 20144178657
a new role for penicillin acylases: degradation of acyl homoserine lactone quorum sensing signals by kluyvera citrophila penicillin g acylase.use of penicillin acylases for the production of semi-synthetic penicillins is well-known. escherichia coli penicillin g acylase (ecpga) has been extensively used for this purpose; however, kluyvera citrophila penicillin g acylase (kcpga) is assumed to be a better substitute, owing to its increased resilience to extreme ph conditions and ease of immobilization. in the present article we report a new dimension for the amidase activity of kcpga by demonstrating its ability to cleave bacterial quor ...201424564895
bacteriophage ϕmam1, a viunalikevirus, is a broad-host-range, high-efficiency generalized transducer that infects environmental and clinical isolates of the enterobacterial genera serratia and kluyvera.members of the enterobacterial genus serratia are ecologically widespread, and some strains are opportunistic human pathogens. bacteriophage ϕmam1 was isolated on serratia plymuthica a153, a biocontrol rhizosphere strain that produces the potently bioactive antifungal and anticancer haterumalide oocydin a. the ϕmam1 phage is a generalized transducing phage that infects multiple environmental and clinical isolates of serratia spp. and a rhizosphere strain of kluyvera cryocrescens. electron micros ...201425107968
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
cloning, overexpression, crystallization and preliminary x-ray crystallographic analysis of a slow-processing mutant of penicillin g acylase from kluyvera citrophila.kluyvera citrophila penicillin g acylase (kcpga) has recently attracted increased attention relative to the well studied and commonly used escherichia coli pga (ecpga) because kcpga is more resilient to harsh conditions and is easier to immobilize for the industrial hydrolysis of natural penicillins to generate the 6-aminopenicillin (6-apa) nucleus, which is the starting material for semi-synthetic antibiotic production. like other penicillin acylases, kcpga is synthesized as a single-chain inac ...201323908045
psychophysical maps for subadditive dissimilarity ratings. 20133684491
resistance and inactivation kinetics of bacterial strains isolated from the non-chlorinated and chlorinated effluents of a wwtp.the microbiological quality of water from a wastewater treatment plant that uses sodium hypochlorite as a disinfectant was assessed. mesophilic aerobic bacteria were not removed efficiently. this fact allowed for the isolation of several bacterial strains from the effluents. molecular identification indicated that the strains were related to aeromonas hydrophila, escherichia coli (three strains), enterobacter cloacae, kluyvera cryocrescens (three strains), kluyvera intermedia, citrobacter freund ...201323924881
correlation between auditory reaction time and intelligence.discriminative reaction times to auditory stimuli were measured for 150 students of the local chinese population. pearson's correlation with scores on the standard progressive matrices and rts was negative, becoming -0.30 when slower trials were eliminated. the possible basis for improvement was discussed in relation to cognitive processes and the general theory of assessing intelligence.20133774443
[functioning and non-functioning tumors of the insular pancreas. pathology, clinical aspects and therapy]. 20134320215
parthenium dermatitis sparing vitiliginous skin. 20133581827
[renal osteodystrophy in children with chronic renal insufficiency]. 20123769023
comparison of segmental pancreatic transplantation with duct obstruction and pancreaticoduodenal transplantation with enteric diversion. 20123313861
some findings on the pathology of the motor nerves of the limbs during medical diseases: the possibility of proximal involvement with conservation of distal excitability. 20124103553
epidemiology of allergic contact sensitization. 20123316993
[properties of lak cell activity and lak-generation in pbl isolated from patients with renal cell carcinoma]. 20123266646
computers and dental health education--a trial project at the liverpool garden festival. 20123297819
biometeorological triggers in childhood asthma.the relationship between child asthma admissions and biometeorological factors was investigated over a 16-month period. no relationship was found with many commonly suspected precipitants such as temperature, humidity and wind. there was however a strong association with rainfall (p much less than 0.001). associations occurred with low barometric pressure and counts of coloured basidiospores and green algae, but no significant relationship was found with grass pollen.20123416418
identification and characterization of two novel bla(kluc) resistance genes through large-scale resistance plasmids sequencing.plasmids are important antibiotic resistance determinant carriers that can disseminate various drug resistance genes among species or genera. by using a high throughput sequencing approach, two groups of plasmids of escherichia coli (named e1 and e2, each consisting of 160 clinical e. coli strains isolated from different periods of time) were sequenced and analyzed. a total of 20 million reads were obtained and mapped onto the known resistance gene sequences. as a result, a total of 9 classes, i ...201223056610
indirect immunofluorescence of oral lichen planus.sections of lesional tissue from 6 patients who exhibited only oral lesions of lichen planus (lp) were incubated with autologous serum and studied immunopathologically for the presence of lichen planus specific antigen (lpsa). none of the specimens was positive for lpsa. serum from the oral lp patients was subsequently incubated with allogeneic cutaneous lp lesional tissue sections, which resulted in the demonstration of lpsa in 2 of the 4 sera examined. these results suggest an immunologic rela ...20113088237
ethanol production from biodiesel-derived crude glycerol by newly isolated kluyvera cryocrescens.the rapidly expanding market for biodiesel has increased the supply and reduced the cost of glycerol, making it an attractive sustainable feed stock for the fuel and chemical industry. glycerol-based biorefinery is the microbial fermentation of crude glycerol to produce fuels and chemicals. a major challenge is to obtain microbes tolerant to inhibitors such as salts and organic solvents present in crude glycerol. microbial screening was attempted to isolate novel strain capable of growing on cru ...201121212944
kluyvera cryocrescens sepsis in a preterm infant.kluyvera cryocrescens, formerly accepted as a benign saprophytic microorganism, is an opportunistic pathogen and its infection is very rare in humans. this report describes a preterm infant born at 30 weeks of gestational age and successfully treated for k. cryocrescens sepsis in the 3rd week of life. to our knowledge, this is the first case of k. cryocrescens sepsis in a newborn. the potential of k. cryocrescens as a serious pathogen should be recognized especially in patients such as preterm i ...201020495273
one-pot, two-step enzymatic synthesis of amoxicillin by complexing with zn2+.a one-pot, two-step enzymatic synthesis of amoxicillin from penicillin g, using penicillin acylase, is presented. immobilized penicillin acylase from kluyvera citrophila was selected as the biocatalyst for its good ph stability and selectivity. hydrolysis of penicillin g and synthesis of amoxicillin from the 6-aminopenicillanic acid formed and d-p-hydroxyphenylglycine methyl ester were catalyzed in situ by a single enzyme. zinc ions can react with amoxicillin to form complexes, and the yield of ...201020567815
neurothermography.neurothermography is a new, clinically useful method of detecting autonomic sympathetic peripheral nerve dysfunction. body symmetry of the skin temperature as maintained by symmetrically functional peripheral nerves is the key to detecting a normal state. persistent 1 degree c temperature asymmetry in the pattern of a peripheral nerve (thermatome) is a highly reliable method of detecting autonomic peripheral nerve disorder.20102845550
ultrathin (1 nm) vertically shadowed platinum-carbon replicas for imaging individual molecules in freeze-etched biological dna and material science metal and plastic specimens.single molecule resolution in beam-sensitive, uncoated, noncrystalline materials has heretofore not been possible except in thin (less than or equal to 150 a) platinum-carbon (pt-c) replicas, which are resistant to electron beam destruction. previously, the granularity of metal film replicas limited their resolution to greater than or equal to 20 a. this paper demonstrates that pt-c film granularity and resolution are a function of the method of replication and other controllable factors. low-an ...20102809773
called to account. 20103105676
genetic transformation of midgut bacteria from the red imported fire ant (solenopsis invicta).in our previous study we isolated 10 bacterial species from fourth-instar larval midguts of the red imported fire ant, solenopsis invicta. here we report the genetic transformation and reintroduction of three species (kluyvera cryocrescens, serratia marcescens, and isolate 38) into the fire ant host. all three species were transformed with the plasmid vector, pzeodsred. high expression levels of dsred were observed and the plasmid is maintained in these bacteria at 37 degrees c in the absence of ...200919159973
antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy.this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ...200919567344
hickman catheter-related bacteremia with kluyvera cryocrescens: a case report.this report describes a 2-year-old child with neuroectodermal tumor presenting with febrile neutropenia. blood cultures drawn from the peripheral vein and hickman catheter revealed kluyvera cryocrescens growth. the hickman catheter was removed and the patient was successfully treated with cefepime and amikacin. isolation of kluyvera spp. from clinical specimens is rare. this saprophyte microorganism may cause serious central venous catheter infections, especially in immunosuppressed patients. cl ...200818503178
genetic and structural insights into the dissemination potential of the extremely broad-spectrum class a beta-lactamase kpc-2 identified in an escherichia coli strain and an enterobacter cloacae strain isolated from the same patient in france.two clinical strains of escherichia coli (2138) and enterobacter cloacae (7506) isolated from the same patient in france and showing resistance to extended-spectrum cephalosporins and low susceptibility to imipenem were investigated. both strains harbored the plasmid-contained bla(tem-1) and bla(kpc-2) genes. bla(kluc-2), encoding a mutant of the chromosomal beta-lactamase of kluyvera cryocrescens, was also identified at a plasmid location in e. cloacae 7506, suggesting the isecp1-assisted escap ...200818625772
the genome and proteome of the kluyvera bacteriophage kvp1--another member of the t7-like autographivirinae.kluyvera, a genus within the family enterobacteriaceae, is an infrequent cause of human infections. bacteriophage kvp1, the only bacteriophage isolated for one of its species, kluyvera cryocrescens, is a member of the viral family podoviridae.200818937848
'the cost benefit of routine pre-operative hb investigations for oral surgery'. 20082493261
kluyvera co-infection in two solid organ transplant recipients: an emerging pathogen or a colonizer bystander?kluyvera species are opportunistic, gram-negative bacilli in the family enterobacteriaceae. ordinarily occurring as a commensal, kluyvera have been reported to cause serious infections in immunosuppressed and immunocompetent hosts, causing diarrhea, urinary infections, peritonitis, and cholecystitis. we report kluyvera infections in 2 solid organ transplant recipients. an 18-year-old female with alpha-1 antitrypsin deficiency underwent living donor liver transplantation and presented 6 months la ...200717313481
effects of induction starting time and ca2+ on expression of active penicillin g acylase in escherichia coli.formation of inclusion bodies is an important obstacle to the production of active recombinant protein in escherichia coli. thus, soluble expression of penicillin g acylase from kluyvera citrophila was investigated in bl21(de3). in this study, the yield of active enzyme was significantly enhanced by the composition of the medium and induction opportunity. when 0.5 mmol/l iptg was added to complex medium at 15 h after incubation, the volumetric and specific activities of penicillin g acylase both ...200717824667
expression and purification of penicillin g acylase enzymes from four different micro-organisms, and a comparative evaluation of their synthesis/hydrolysis ratios for cephalexin.several genes for the enzyme penicillin g acylase, as isolated from four different micro-organisms (alcaligenes facaelis, escherichia coli, kluyvera cryocrescens or providencia rettgeri) were modified at their carboxy-termini to include his-tag fusions, then were expressed from the plasmid pet-24a(+) in e. coli jm109(de3) cells. all fusion proteins were next purified to homogeneity in a single step by agar-based co-ida chromatography, and were then evaluated as catalysts for the synthesis of cep ...200616139515
effect of organic cosolvent on kinetic resolution of tert-leucine by penicillin g acylase from kluyvera citrophila.penicillin g acylase (pga) from kluyvera citrophila immobilized on amberzyml was used for enantioselective hydrolysis of n-phenylacetylated-dl-tert-leucine (n-phac-dl-tle) to produce l-tert-leucine (l-tle). the effects of various organic cosolvents on hydrolysis of n-phac-dl-tle have been investigated in aqueous-cosolvent medium. it was founded that the rate of pga-catalyzed reaction was significantly affected by the presence of 2% (v/v) organic cosolvent concentration. the initial rate fell wit ...200616245080
preparation of optically pure tert-leucine by penicillin g acylase-catalyzed resolution.penicillin g acylase, from kluyvera citrophila, was used in kinetic resolution of dl-tert-leucine. n-phenylacetylated-dl-tert-leucine, chemically synthesized from dl-tert-leucine, was enantioselctively hydrolyzed by penicillin g acylase to obtain l-tert-leucine, d-tert-leucine was prepared by acid-catalyzed hydrolysis of the remaining substrate. the total yields of d-tert-leucine and l-tert-leucine are 80.6% and 83.1%, respectively. the enantiomeric excess of the two products, d-tert-leucine and ...200616707334
a framework for the knowledge-based interpretation of laboratory data in intensive care units using deductive database technology.in co-operation with the institute of anaesthesiology of the ludwig-maximilians-university in munich a computer-based system for the analysis and interpretation of renal function, fluid and electrolyte metabolism of critical care patients has been developed. this paper focuses on the requirements and implementation aspects of the knowledge-based interpretation for this particular system. objective of the proposed approach is, to transform an enormous--and constantly increasing--amount of raw dat ...20061482854
hyperoncology: demonstration of an evolving comprehensive hypermedia-based workstation for the radiation oncologist.hyperoncology is a prototype hypermedia-based workstation designed for the practicing radiation oncologist. individual patient records consist of linked demographic, clinical history, presentation, staging, course, laboratory and treatment data. searching and sorting capabilities, navigational aids, information printing and archiving, plotting capability, and graphics and updating tools are available. upon integration with an evolving knowledge base, hyperoncology will eventually become a compre ...20061483010
a review of the safety profile of teicoplanin.safety of teicoplanin has been assessed in 3377 patients treated in europe up to the end of june 1990. one or more adverse events were experienced by 10% of patients. age and teicoplanin dose had no significant effect on the incidence or type of adverse event. in comparative trials the incidence and profile of adverse events to teicoplanin have been similar to those seen with beta-lactam therapy. impaired renal function occurred consistently more frequently with vancomycin therapy than with teic ...20061829079
health checks on patients 75 years and over in nottinghamshire after the new gp contract.to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.20061393076
[association of monoclonal gammopathy with chronic glomerulonephritis and autoimmune hyperlipemia. course of disease during therapy].a case of benign monoclonal gammapathy associated with an auto-immune hyperlipidaemia and a chronic glomerulopathy is reported. extraction of the monoclonal immunoglobulin made it possible to demonstrate its anti-lipoprotein antibody activity. parallel changes in serum lipid levels and monoclonal immunoglobulin levels under the influence of melphalan confirmed the direction relationship between the lipid and protein disorders. the pathogenesis of the glomerulopathy and of the massive and prolong ...20051068625
[kartagener's syndrome in two siblings]. 20051087624
[fractures and luxations in the pelvic region. urogenital injuries (author's transl)]. 2005538870
phylogenetic relationships of the genus kluyvera: transfer of enterobacter intermedius izard et al. 1980 to the genus kluyvera as kluyvera intermedia comb. nov. and reclassification of kluyvera cochleae as a later synonym of k. intermedia.in order to assess the relationship between the genus kluyvera and other members of the family enterobacteriaceae, the 16s rrna genes of type strains of the recognized kluyvera species, kluyvera georgiana, kluyvera cochleae, kluyvera ascorbata and kluyvera cryocrescens, were sequenced. a comparative phylogenetic analysis based on these 16s rrna gene sequences and those available for strains belonging to several genera of the family enterobacteriaceae showed that members of the genus kluyvera for ...200515653915
natural antimicrobial susceptibility patterns of kluyvera ascorbata and kluyvera cryocrescens strains and review of the clinical efficacy of antimicrobial agents used for the treatment of kluyvera infections.the natural susceptibility of 58 k. ascorbata and 24 k. cryocrescens strains to 71 antimicrobial agents was investigated. mic values were determined with a microdilution procedure in cation-adjusted mueller hinton broth (for all strains) and isosensitest broth (for some strains). both species were naturally sensitive or of intermediate susceptibility to tetracyclines, aminoglycosides, quinolones, antifolates, chloramphenicol, nitrofurantoin, fosfomycin, aminopenicillins plus beta-lactamase inhib ...200515920899
cloning, overexpression, and characterization of a novel thermostable penicillin g acylase from achromobacter xylosoxidans: probing the molecular basis for its high thermostability.the gene encoding a novel penicillin g acylase (pga), designated pgaw, was cloned from achromobacter xylosoxidans and overexpressed in escherichia coli. the pgaw gene contains an open reading frame of 2586 nucleotides. the deduced protein sequence encoded by pgaw has about 50% amino acid identity to several well-characterized pgas, including those of providencia rettgeri, kluyvera cryocrescens, and escherichia coli. biochemical studies showed that the optimal temperature for this novel pga (pga6 ...200415128530
expression, purification, and characterization of his-tagged penicillin g acylase from kluyvera citrophila in escherichia coli.the dna fragment encoding kluyvera citrophila penicillin g acylase (kcpga) was amplified and cloned into the vector pet28b to obtain a c-terminus his-tagged fusion expression plasmid. the fusion protein kcpga was successfully overexpressed in escherichia coli bl21(de3). the optimal induction concentration of isopropylthio-beta-d-galactoside (iptg) was found to be 5 microm. the fusion protein was purified in a single step by ni-ida affinity chromatograph to a specific activity of 35.3u/mg protein ...200415477078
evidence for the involvement of arginyl residue at the active site of penicillin g acylase from kluyvera citrophila.penicillin g acylase (pga) is used for the commercial production of semi-synthetic penicillins. it hydrolyses the amide bond in penicillin producing 6-aminopenicillanic acid and phenylacetate. 6-aminopenicillanic acid, having the beta-lactam nucleus, is the parent compound for all semi-synthetic penicillins. penicillin g acylase from kluyvera citrophila was purified and chemically modified to identify the role of arginine in catalysis. modification with 20 mm phenylglyoxal and 50 mm 2,3-butanedi ...200415604805
[ultrasonic localization for closed renal biopsy]. 2004520913
a controlled study of the perception of body width in anorexia nervosa. 2004529199
echocardiographic features of right ventricular volume overload. 2004529179
intra-osseous lipoma of the proximal part of the femur. case report. 2004374417
health care in china. 1. 2004310189
health care in china. 2. 2004310187
polymyxins as inhibitors of polyclonal b-cell activators in murine lymphocyte cultures.the polymyxin antibiotics polymyxin b sulfate and colistin methane sulfonate were examined for their ability to inhibit responses to the polyclonal b-cell activators (pba) bacterial lipopolysaccharide (lps), dextran sulfate (ds), pneumococcal polysaccharide (siii), and purified protein derivative of tuberculin (ppd) in spleen cell cultures. polymyxin concentrations of 1 and 10 microng/ml significantly inhibited both the deoxyribonucleic acid synthetic and polyclonal antibody responses stimulated ...2003193787
[melanotic progonoma. report of two cases (author's transl)]. 2003182628
resection of a massive arteriovenous malformation of the head and neck.we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case.2003154679
polymorphism in the yclc-rpos region of enterobacteria.the nucleotide sequence downstream from the rpos gene in enterobacter cloacae and kluyvera cryocrescens contains the slya-pad1-yclc genes. the dna sequence of enterobacter cloacae cetc960 shows a 2.6-kb insertion of unknown origin between rpos and slya. this 2.6-kb sequence has also been detected in species of salmonella and in pseudomonas aeruginosa, but not in the same location. this insertion has been detected in all the enterobacter cloacae clinical strains studied although the size of the r ...200312732965
improving the specific synthetic activity of a penicillin g acylase using dna family shuffling.penicillin g acylas (pga) of providencia rettgeri (atcc 25599) was evolved using a modified dna family shuffling method. the identity of pga genes from escherichia coli, kluyvera citrophila and providencia rettgeri ranges from 62.5% to 96.9%. the pga genes from above three species were recombined and shuffled to create interspecies pga gene fusion libraries. by substituting assembled chimaeras for corresponding region of petppga, different recombinants were constructed and expressed in e. coli j ...200312796820
nosocomial outbreak of kluyvera cryocrescens bacteremia. 200211893149
identification of a chromosome-borne expanded-spectrum class a beta-lactamase from erwinia persicina.from whole-cell dna of an enterobacterial erwinia persicina reference strain that displayed a penicillinase-related antibiotic-resistant phenotype, a beta-lactamase gene was cloned and expressed in escherichia coli. it encoded a clavulanic-acid-inhibited ambler class a beta-lactamase, erp-1, with a pi value of 8.1 and a relative molecular mass of ca. 28 kda. erp-1 shared 45 to 50% amino acid identity with the most closely related enzymes, the chromosomally encoded enzymes from citrobacter koseri ...200212384342
[some epidemiological factors in the incidence of female genital cancer in the azerbaijan ssr].in the structure of cancer morbidity of the female genitalia in azerbaijan ssr within a ten-year period (1957-1966) cervical cancer occupies the first place--86.6%, it is observed more frequently among russian female population (46.4 per 100 000 wives) and more rarely among aboriginal female population (7.1 per 100 000 wives). of 2488 patients with cervical cancer 24.5% showed previous lesions against which background cancer would arise. patients with cervical ruptures, advanced erosions, polyps ...2002127423
v. brown university alcoholism conference: a summary of the discussions. 2002127377
fluorogenic assay for penicillin g acylase activity.a simple, highly sensitive, and rapid assay for high-throughput screening of penicillin g acylase-producing bacteria is presented. the method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin g acylase. the present method is suitable for screening pure enzymes as well as various penicillin g acylases like those from escherichia coli, proteus rettgeri, and kluyvera citrophila in cell extracts. in addi ...200111355855
further studies on rpos in enterobacteria: identification of rpos in enterobacter cloacae and kluyvera cryocrescens.rpos, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. to determine the presence of this gene in enteric bacteria, an escherichia coli rpos probe was constructed and used to detect the presence of this gene in different species. a gene homologous to rpos was found in citrobacter amalonaticu ...200111491080
characterization of a chromosomally encoded extended-spectrum class a beta-lactamase from kluyvera cryocrescens.a chromosomally located beta-lactamase gene, cloned and expressed in escherichia coli from a reference strain of the enterobacterial species kluyvera cryocrescens, encoded a clavulanic acid-inhibited ambler class a enzyme, kluc-1, with a pi value of 7.4. kluc-1 shared 86% amino acid identity with a subgroup of plasmid-mediated ctx-m-type extended-spectrum beta-lactamases (ctx-m-1, -3, -10, -11, and -12), the most closely related enzymes, and 77% amino acid identity with klua-1 from kluyvera asco ...200111709346
outbreak of nosocomial infections due to extended-spectrum beta-lactamase-producing strains of enteric group 137, a new member of the family enterobacteriaceae closely related to citrobacter farmeri and citrobacter amalonaticus.a member of the enterobacteriaceae initially identified as kluyvera cryocrescens by the microscan gram-negative combo 13 panel caused an outbreak of nosocomial infections in four patients (pneumonia, n = 2; urinary tract infection, n = 1; wound infection, n = 1) and urinary tract colonization in one patient. when the strains were tested by the enteric reference laboratory of the centers for disease control and prevention, biochemical results were most compatible with yersinia intermedia, kluyver ...200011060050
[kluyvera cryocrescens: a positive urine culture in a young girl with persistent proteinuria].kluyvera genus usually shows two kinds of species: k. ascorbata and k. cryocrescens, dna hybridization let us to differentiate a third group: kluyvera species 3. its diagnosis is quite uncommon and its taxonomy have been recently clarified. we report here a ten years female record with a chronic proteinuria and a positive urine-culture for k. cryocrescens. axetil cefuroxime treatment was absolutely succesful. kluyvera infections are difficult on the whole to joint with some specific clinical fea ...199910464962
the use of chromogenic reference substrates for the kinetic analysis of penicillin acylases.determination of kinetic parameters of penicillin acylases for phenylacetylated compounds is complicated due to the low k(m) values for these substrates, the lack of a spectroscopic signal, and the strong product inhibition by phenylacetic acid. to overcome these difficulties, a spectrophotometric method was developed, with which kinetic parameters could be determined by measuring the effects on the hydrolysis of the chromogenic reference substrate 2-nitro-5-[(phenylacetyl)amino]benzoic acid (ni ...199910542108
kluyvera cryocrescens finger infection: case report and review of eighteen kluyvera infections in human beings.we report a case of soft tissue infection with kluyvera cryocrescens and a critical review of kluyvera infections. a 31-year-old diabetic man used a new chemical for stripping the floor with his bare hands. two days later he developed a blister on a finger which progressed to tenosynovitis in spite of intravenous nafcillin therapy. after 11 days culture and sensitivity results dictated treatment with intravenous ticarcillin/clavulanic acid. the wound was debrided twice, and later a skin flap was ...19989884842
identification of the pac promoter from kluyvera citrophila.the nucleotide sequence of the 5'-terminal region of the pac gene encoding the penicillin g acylase from kluyvera citrophila atcc 21285 has been determined. the transcriptional start site has been identified by primer extension analysis in a different position to that previously found for the homologous pac gene of escherichia coli w atcc 11105. two nucleotide changes in the -35 box appear to be responsible of the promoter displacement in k. citrophila. a putative upstream promoter element (a+t- ...19979198278
kluyvera bacteriophage kvp1: a new member of the podoviridae family phylogenetically related to the coliphage t7.a dna containing bacteriophage, kvp1, was isolated from the water of a very polluted river, the matanza river, near the central district of buenos aires city. this bacteriophage infects bacteria belonging to the kluyvera cryocrescens species (strain 21 g) isolated from the same river. kvp1 is a lytic bacteriophage and its propagation characteristics are: burst size 30, latent period 13 min and rise period 10 min. morphologically, kvp1 is a small icosahedral bacteriophage, 59.1 nm in diameter, wh ...19979381794
rapid burst kinetics in the hydrolysis of 4-nitrophenyl acetate by penicillin g acylase from kluyvera citrophila. effects of mutation f360v on rate constants for acylation and de-acylation.the kinetics of release of 4-nitrophenol were followed by stopped-flow spectrophotometry with two 4-nitrophenyl ester substrates of penicillin g acylase from kluyvera citrophila. with the ester of acetic acid, but not of propionic acid, there was a pre-steady-state exponential phase, the kinetics of which were inhibited by phenylacetic acid (a product of hydrolysis of specific substrates) to the extent predicted from ki values. this was interpreted as deriving from rapid formation (73 mm-1.s-1) ...19968687381
a case of kluyvera cryocrescens peritonitis in a capd patient. 19968761550
[utility of pyrrolidonyl-arylamidase detection for typing enterobacteriaceae and non-fermenting gram-negative bacteria].detection of pyrrolidonyl-aryl-amidase activity (pyr) is an important tool to identify gram-positive cocci, such as staphylococci, enterococci, streptococci, and other related genera. however, only few studies evaluating its usefulness with gram-negative rods have been published. thus, a prospective study including 542 and 215 unique clinical isolates of enterobacteriaceae and non-fermentative gram-negative rods, respectively, was undertaken. strains were identified by conventional methods. pyr ...19958850133
new insights on the specificity of penicillin acylase.in contrast with the general thought that penicillin g acylases (pgas) were only able to hydrolyse amides or esters of higly hydrophobic acids, we have demonstrated that the pga from kluyvera citrophila catalysed the hydrolysis of 4-nitrophenyl esters of acetic, propionic, butyric and valeric acids. values of kcat. and kcat./km were greatest for the first compound and less than values for benzylpenicillin by factors of 30 and 7, respectively. 4-nitrophenyl acetate was hydrolysed faster than 2-ni ...19957826381
changing the substrate specificity of penicillin g acylase from kluyvera citrophila through selective pressure.escherichia coli (mut, mutd, leu-) cells transformed with plasmid pykd59 harbouring the pac gene encoding penicillin acylase (pa) from kluyvera citrophila atcc 21285 were exposed to environmental conditions that made expression of this enzyme essential for growth. under these conditions, spontaneous mutants were isolated that used adipyl-l-leucine as the sole source of l-leucine. dna sequencing of the mutant pac genes identified a transversion mutation of thymine to guanine at position 1163. thi ...19947980457
ph studies to elucidate the chemical mechanism of penicillin acylase from kluyvera citrophila.the variation with ph of the kinetic parameters of penicillin acylase from kluyvera citrophila has been used to gain information about the chemical mechanism of the reaction catalysed by the enzyme. the ph-dependence of log (v/km) for penicillin g showed that a group with a pk value over 4.7 must be deprotonated and that a group with a pk value over 9.7 must be protonated in the free enzyme for activity. the solvent perturbation and temperature studies indicated that these groups are respectivel ...19938338640
characterization of an escherichia coli aromatic hydroxylase with a broad substrate range.the hpab gene encoding an aromatic hydroxylase of escherichia coli atcc 11105, a penicillin g acylase-producing strain, has been cloned and expressed in e. coli k-12. this gene was located near the paca gene coding for penicillin g acylase. the hydroxylase has a molecular mass of 59,000 da, uses nadh as a cosubstrate, and was tentatively classified as a 4-hydroxyphenylacetic acid hydroxylase, albeit it exhibited a rather broad substrate specificity acting on different monohydric and dihydric phe ...19938458860
inactivation of penicillin acylase from kluyvera citrophila by n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline: a case of time-dependent non-covalent enzyme inhibition.penicillin acylase (pa) from kluyvera citrophila was inhibited by n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (eedq), a specific carboxy-group-reactive reagent. enzyme activity progressively decreased to a residual value depending on eedq concentration. neither enzymic nor non-enzymic decomposition of eedq is concomitant with pa inactivation. moreover, enzyme re-activation is achieved by chromatographic removal of eedq, ph increase or displacement of the reagent with penicillin g. it was then ...19938489517
stabilization of heterodimeric enzyme by multipoint covalent immobilization: penicillin g acylase from kluyvera citrophila.we have developed a strategy for immobilization-stabilization of penicillin g acylase (pga) from kluyvera citrophila by controlled multipoint covalent attachment to agarose-aldehyde gels. this enzyme is composed by two dissimilar subunits noncovalently bound. thus, in this article we establish clear correlations between enzyme stabilization and the multipoint immobilization and/or between enzyme stabilization and the involvement of the two subunits in the attachment of them to the support. we ha ...199318613049
changing glycine 21 for glutamic acid in the beta-subunit of penicillin g acylase from kluyvera citrophila prevents protein maturation.active penicillin acylase from kluyvera citrophila strain atcc 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. using the chemical mutagen hydroxylamine we have treated plasmid pykd59 containing the active penicillin acylase gene (pga) from k. citrophila and have generated different point mutant penicillin acylase genes, one producing a maturation deficient precursor. this point mutation has changed the glycine 310 residu ...19921368070
sleep organization and energy expenditure of breast-fed and formula-fed infants.sleep organization of infants may be influenced by differences in nutrient intakes from human milk and formula. because sleep/awake and sleep stage patterns affect energy expenditure, we hypothesized that differences in sleep organization between breast-fed and formula-fed infants might account in part for differences in energy expenditure between feeding groups. sleep stages and cycling of 4-mo-old breast-fed (n = 10) formula-fed (n = 10) infants were studied with simultaneous measurements of e ...19921480450
chemical modification of serine at the active site of penicillin acylase from kluyvera citrophila.the site of reaction of penicillin acylase from kluyvera citrophila with the potent inhibitor phenylmethanesulphonyl fluoride was investigated by incubating the inactivated enzyme with thioacetic acid to convert the side chain of the putative active-site serine residue to that of cysteine. the protein product contained one thiol group, which was reactive towards 2,2'-dipyridyl disulphide and iodoacetic acid. carboxymethylcysteine was identified as the n-terminal residue of the beta-subunit of th ...19911764029
thermodynamic profiles of penicillin g hydrolysis catalyzed by wild-type and met----ala168 mutant penicillin acylases from kluyvera citrophila.the met-168 residue in penicillin acylase from kluyvera citrophila was changed to ala by oligonucleotide site-directed mutagenesis. the ala-168 mutant exhibited different substrate specificity than wild-type and enhanced thermal stability. the thermodynamic profiles for penicillin g hydrolysis catalyzed by both enzymes were obtained from the temperature dependence of the steady-state kinetic parameters km and kcat. the high values of enthalpy and entropy of activation determined for the binding ...19902306469
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