Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| proceedings of réanimation 2017, the french intensive care society international congress. | 2017 | 28074416 | |
| proceedings of réanimation 2017, the french intensive care society international congress. | 2017 | 28074415 | |
| prospective surveillance of healthcare associated infections in a cambodian pediatric hospital. | healthcare associated infections (hai) are the most common preventable adverse events following admission to healthcare facilities. data from low-income countries are scarce. we sought to prospectively define hai incidence at angkor hospital for children (ahc), a cambodian pediatric referral hospital. | 2017 | 28138385 |
| evaluation of three sample preparation methods for the direct identification of bacteria in positive blood cultures by maldi-tof. | patient mortality is significantly reduced by rapid identification of bacteria from sterile sites. maldi-tof can identify bacteria directly from positive blood cultures and multiple sample preparation methods are available. we evaluated three sample preparation methods and two maldi-tof score cut-off values. positive blood culture bottles with organisms present in gram stains were prospectively analysed by maldi-tof. three lysis reagents (saponin, sds, and sepsityper lysis bufer) were applied to ... | 2017 | 28100271 |
| abstracts. | 2017 | 28044356 | |
| whole genome sequencing reveals high-resolution epidemiological links between clinical and environmental klebsiella pneumoniae. | klebsiella pneumoniae is a gram-negative bacterial species capable of occupying a broad range of environmental and clinical habitats. known as an opportunistic pathogen, it has recently become a major causative agent of clinical infections worldwide. despite growing knowledge about the highly diverse population of k. pneumoniae, the evolution and clinical significance of environmental k. pneumoniae, as well as the relationship between clinical and environmental k. pneumoniae, are poorly defined. | 2017 | 28118859 |
| development of an algorithm for phenotypic screening of carbapenemase-producing enterobacteriaceae in the routine laboratory. | carbapenemase-producing enterobacteriaceae (cpe) are difficult to identify among carbapenem non-susceptible enterobacteriaceae (nse). we designed phenotypic strategies giving priority to high sensitivity for screening putative cpe before further testing. | 2017 | 28095794 |
| the influence of antibiotic prophylaxis on bacterial resistance in urinary tract infections in children with spina bifida. | bacterial resistance to antibiotics is an increasingly threatening consequence of antimicrobial exposure for many decades now. in urinary tract infections (utis), antibiotic prophylaxis (ap) increases bacterial resistance. we studied the resistance patterns of positive urinary cultures in spina bifida children on clean intermittent catheterization, both continuing and stopping ap. | 2017 | 28081719 |
| development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of listeria monocytogenes. | listeria monocytogenes, one of most problematic foodborne pathogens, is responsible for listeriosis in both humans and animals and mainly transmitted through the food chain. in this report, we propose a simple, rapid, and nearly instrument-free molecular technique using multiple cross displacement amplification (mcda) label-based gold nanoparticles lateral flow biosensor (lfb) for specific, sensitive, and visual detection of l. monocytogenes. the mcda-lfb method was carried out at a constant tem ... | 2017 | 28138243 |
| induced membrane technique for the treatment of chronic hematogenous tibia osteomyelitis. | chronic hematogenous osteomyelitis often results from the improper treatment of acute hematogenous osteomyelitis. at present, there is lack of uniform standards for the treatment, and the clinical features of the disease are unclear. the purpose of this study was to explore the clinical efficacy and complications of chronic hematogenous tibia osteomyelitis treated with the induced membrane technique. | 2017 | 28114929 |
| risk factors for relapse or persistence of bacteraemia caused by enterobacter spp.: a case-control study. | enterobacter spp. possess chromosomal ampc beta-lactamases that may be expressed at high levels. previous studies have demonstrated a risk of relapsed bacteraemia following therapy with third generation cephalosporins (3gcs). what additional factors predict microbiological failure in enterobacter bacteraemia is unclear. we aimed to determine factors associated with microbiological failure in enterobacter bacteraemia. | 2017 | 28127422 |
| fungal periprosthetic joint infection following total elbow arthroplasty: a case report and review of the literature. | with improving surgical techniques for total elbow arthroplasty clinical outcomes have improved and its utilization continues to increase. despite these advances, complication rates remain as high as 24%. of these complications periprosthetic joint infection is one of the most common and morbid. the rheumatoid elbow remains a leading indication for total elbow arthroplasty. patients with this condition frequently require immunosuppressive therapy, which places them at higher risk of both typical ... | 2017 | 28109195 |
| comparison of 16s ribosomal rna gene sequence analysis and conventional culture in the environmental survey of a hospital. | nosocomial infection is one of the most common complications within health care facilities. certain studies have reported outbreaks resulting from contaminated hospital environments. although the identification of bacteria in the environment can readily be achieved using culturing methods, these methods detect live bacteria. sequencing of the 16s ribosomal rna (16s rrna) gene is recognized to be effective for bacterial identification. in this study, we surveyed wards where drug-resistant bacteri ... | 2017 | 28116119 |
| global assessment of the activity of tigecycline against multidrug-resistant gram-negative pathogens between 2004 and 2014 as part of the tigecycline evaluation and surveillance trial. | multidrug-resistant (mdr) gram-negative organisms are a burden on the global health care system. the tigecycline evaluation and surveillance trial (test) is an ongoing global study designed to monitor the in vitro activities of tigecycline and a panel of marketed antimicrobials against a range of clinically significant pathogens. in this study, in vitro data are presented for mdr acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterob ... | 2017 | 28124025 |
| bacterial plasmid-mediated quinolone resistance genes in aquatic environments in china. | emerging antimicrobial resistance is a major threat to human's health in the 21(st) century. understanding and combating this issue requires a full and unbiased assessment of the current status on the prevalence of antimicrobial resistance genes and their correlation with each other and bacterial groups. in aquatic environments that are known reservoirs for antimicrobial resistance genes, we were able to reach this goal on plasmid-mediated quinolone resistance (pmqr) genes that lead to resistanc ... | 2017 | 28094345 |
| microrna-155 is upregulated in ascites in patients with spontaneous bacterial peritonitis. | mircorna's (mir) have been recognised as important modulators of gene expression and potential biomarkers. however, they have been rarely investigated in bio fluids apart from blood. we investigated the association of mir-125b and mir-155 with complications of cirrhosis. ascites was prospectively collected from patients with cirrhosis undergoing paracentesis at our department. mir's were determined in the supernatant using qpcr and normalized by sv-40. clinical parameters were assessed at parace ... | 2017 | 28074870 |
| development of a recombinase polymerase amplification assay for detection of epidemic human noroviruses. | human norovirus is a leading cause of viral gastroenteritis worldwide. rapid detection could facilitate control, however widespread point-of-care testing is infrequently done due to the lack of robust and portable methods. recombinase polymerase amplification (rpa) is a novel isothermal method which rapidly amplifies and detects nucleic acids using a simple device in near real-time. an rt-rpa assay targeting a recent epidemic human norovirus strain (gii.4 new orleans) was developed and evaluated ... | 2017 | 28067278 |
| antibacterial activities of the methanol extract, fractions and compounds from elaeophorbia drupifera (thonn.) stapf. (euphorbiaceae). | elaeophorbia drupifera (thonn.) stapf. (euphorbiaceae) is used in cameroonian folk medicine to treat several ailments including bacterial-related diseases such as skin infections. in this study, the methanol extract from the leaves (edl), fractions (edla-d), sub-fractions edlc1-7 and edlc31-35 as well as isolated compounds were tested for their antimicrobial activities against a panel of gram-negative and gram-positive bacteria including multidrug resistant (mdr) phenotypes. | 2017 | 28061888 |
| risk factors and characteristics of blood stream infections in patients with newly diagnosed multiple myeloma. | patients with multiple myeloma are generally immune-compromised either due to pronounced depression in primary antibody responses or because of anti-myeloma therapy. infection is a major risk factor for early deaths among these patients. the impact of blood stream infections (bsi) on newly diagnosed myeloma patients has been less studied. we aimed to study the incidence and risk factors of bsi within 3 months after diagnosis of multiple myeloma in a tertiary referral center. | 2017 | 28056867 |
| prevalence of multidrug-resistant organisms in refugee patients, medical tourists and domestic patients admitted to a german university hospital. | patients with contact to healthcare-system in high-prevalence countries (hpc) and refugee patients in hospital settings (ref) have previously been identified to be at risk of carrying multidrug-resistant organisms (mdro). comparative studies addressing the epidemiology of mdro in patients transferred from hospitals abroad (abroad) and ref are lacking but are necessary to introduce refined infection control measures. | 2017 | 28056820 |
| identification and whole genome sequencing of the first case of kosakonia radicincitans causing a human bloodstream infection. | the taxonomy of enterobacter species is rapidly changing. herein we report a bloodstream infection isolate originally identified as enterobacter cloacae by vitek2 methodology that we found to be kosakonia radicincitans using genetic means. comparative whole genome sequencing of our isolate and other published kosakonia genomes revealed these organisms lack the ampc β-lactamase present on the chromosome of enterobacter sp. a fimbriae operon primarily found in escherichia coli o157:h7 isolates was ... | 2017 | 28174569 |
| activity of cefiderocol (s-649266) against carbapenem-resistant gram-negative bacteria collected from inpatients in greek hospitals. | cefiderocol (s-649266), a siderophore cephalosporin, utilizes a novel mechanism of entry into the periplasmic space of gram-negative bacteria and is broadly stable to esbls and carbapenemases. | 2017 | 28369471 |
| n6', n6''', and o4' modifications to neomycin affect ribosomal selectivity without compromising antibacterial activity. | the synthesis of a series of neomycin derivatives carrying the 2-hydroxyethyl substituent on n6' and/or n6‴ both alone and in combination with a 4'-o-ethyl group is described. by means of cell-free translation assays with wild-type bacterial ribosomes and their hybrids with eukaryotic decoding a sites, we investigate how individual substituents and their combinations affect activity and selectivity at the target level. in principle, and as shown by cell-free translation assays, modifications of ... | 2017 | 28343384 |
| new mouse model for chronic infections by gram-negative bacteria enabling the study of anti-infective efficacy and host-microbe interactions. | only a few, relatively cumbersome animal models enable long-term gram-negative bacterial infections that mimic human situations, where untreated infections can last for weeks. here, we describe a simple murine cutaneous abscess model that enables chronic or progressive infections, depending on the subcutaneously injected bacterial strain. in this model, pseudomonas aeruginosa cystic fibrosis epidemic isolate lesb58 caused localized high-density skin and soft tissue infections and necrotic skin l ... | 2017 | 28246361 |
| antibacterial effect of sevoflurane and isoflurane. | multidrug resistant bacteria are increasing worldwide and therapeutic options are limited. some anaesthetics have shown antibacterial activity before. in this study, we have investigated the antibacterial effect of the halogenated anaesthetic agents sevoflurane and isoflurane against a range of resistant pathogens. | 2017 | 28198170 |
| in vitro screening of an fda-approved library against eskape pathogens. | bacterial resistance to conventional antibiotics is an increasingly serious threat to public health worldwide that requires immediate exploration and the development of novel antimicrobial compounds. drug repurposing is an inexpensive and untapped source of new antimicrobial leads, and it holds many attractive features warranting further attention for antimicrobial drug discovery. in an effort to repurpose drugs and explore new leads in the field of antimicrobial drug discovery, we performed a w ... | 2017 | 28190396 |
| use of colistin in a neonatal intensive care unit: a cohort study of 65 patients. | background the emergence of infections related to multidrug-resistant gram-negative bacilli (mdr-gnb) reintroduced the use of colistin, an antibiotic that was previously abandoned due to adverse effects. however, because of its limited use in neonatal intensive care units, there is very little data about the effectiveness and safety of colistin in children and newborns. in this study, which will be the largest case study in the literature, we aimed to evaluate the effectiveness and safety of col ... | 2017 | 28135233 |
| antimicrobial activity, ame resistance, and a-site binding studies of anthraquinone-neomycin conjugates. | the antibacterial effects of aminoglycosides are based on their association with the a-site of bacterial rrna and interference with the translational process in the bacterial cell, causing cell death. the clinical use of aminoglycosides is complicated by resistance and side effects, some of which arise from their interactions with the human mitochondrial 12s rrna and its deafness-associated mutations, c1494u and a1555g. we report a rapid assay that allows screening of aminoglycoside compounds to ... | 2017 | 28103015 |
| rectal carriage of extended-spectrum beta-lactamase and carbapenemase producing gram-negative bacilli in intensive care units in tunisia. | this study was conducted to evaluate the rate of fecal carriage of gram-negative bacilli (gnb) resistant to third-generation cephalosporins (third gc) in patients hospitalized in the intensive care unit (icu) of charles nicolle hospital of tunis and to identify the enzymatic mechanisms involved. from february to april 2014, rectal swabs were collected from all patients (n = 38) at admission and once weekly thereafter to identify acquisition. they were cultured on desoxycholate-lactose-agar plate ... | 2017 | 28099062 |
| clonal transmission of gram-negative bacteria with carbapenemases ndm-1, vim-1, and oxa-23/72 in a bulgarian hospital. | we characterized 72 isolates with reduced susceptibility to carbapenems (50 acinetobacter spp., 13 proteus mirabilis, five escherichia coli, one morganella morganii, one enterobacter cloacae, one providencia rettgeri, and one pseudomonas aeruginosa) from a hospital in sofia, bulgaria. different β-lactamase genes were identified by polymerase chain reaction and sequencing. bacterial strain typing was performed by enzymatic macrorestriction and pulsed-field gel electrophoresis (pfge) typing as wel ... | 2017 | 27459019 |
| in vitro activity of a polyhexanide-betaine solution against high-risk clones of multidrug-resistant nosocomial pathogens. | to determine the in vitro activity of a polyhexanide-betaine solution against collection strains and multidrug-resistant (mdr) nosocomial isolates, including high-risk clones. | 2017 | 27004429 |
| analysis of microbial diversity in shenqu with different fermentation times by pcr-dgge. | shenqu is a fermented product that is widely used in traditional chinese medicine (tcm) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. the aim of this study was to investigate microbial diversity in shenqu using different fermentation time periods. dgge (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated that a strain of pediococcus acidilactici (band 9) is the predominant bacteria during fermentation and tha ... | 2017 | 28256389 |
| the essential oil from the fruits of the brazilian spice xylopia sericea a. st.-hil. presents expressive in-vitro antibacterial and antioxidant activity. | the aims of this study were to investigate the chemical composition and the antioxidant activity and antibacterial activity of the essential oil of xylopia sericea fruits (oxs). the fruits of this species are popularly used for medicinal purposes, and as a condiment in food preparation. | 2017 | 28134988 |
| screening of mercury-resistant and indole-3-acetic acid producing bacterial-consortium for growth promotion of cicer arietinum l. | mercury resistant (hg(r) ) bacteria were screened from industrial effluents and effluents-polluted rhizosphere soils near to districts kasur and sheikhupura, pakistan. out of 60 isolates, three bacterial strains, bacillus sp. az-1, bacillus cereus az-2, and enterobacter cloacae az-3 showed hg-resistance as 20 μg ml(-1) of hgcl2 and indole-3-acetic acid (iaa) production as 8-38 μg ml(-1) . biochemical and molecular characterization of selected bacteria was confirmed by 16s ribotyping. mercury res ... | 2017 | 27911010 |
| peptidomics of acanthoscurria gomesiana spider venom reveals new toxins with potential antimicrobial activity. | acanthoscurria gomesiana is a brazilian spider from the theraphosidae family inhabiting regions of southeastern brazil. potent antimicrobial peptides as gomesin and acanthoscurrin have been discovered from the spider hemolymph in previous works. spider venoms are also recognized as sources of biologically active peptides, however the venom peptidome of a. gomesiana remained unexplored to date. in this work, a ms-based workflow was applied to the investigation of the spider venom peptidome. data- ... | 2017 | 27436114 |
| prevalence, antibiotic susceptibility and characterization of antibiotic resistant genes among carbapenem-resistant gram-negative bacilli and yeast in intestinal flora of cancer patients in north lebanon. | the emergence and spread of carbapenem-resistant bacteria are a significant clinical and public health concern. the aim of the study is to determine the prevalence of intestinal carriage of carbapenem-resistant bacteria and yeasts in cancer patients under chemotherapy. 41 stool samples collected from cancer patients in nini hospital in tripoli, north lebanon have been analyzed. after isolating yeasts and carbapenem-resistant bacteria, a biochemical identification and antimicrobial susceptibility ... | 2017 | 28216021 |
| neonatal bacterial conjunctivitis in korea in the 21st century. | to investigate the microbial cause and antibiotic susceptibility of neonatal bacterial conjunctivitis in korean patients in the 21st century. | 2017 | 28002109 |
| simplified and representative bacterial community of maize roots. | plant-associated microbes are important for the growth and health of their hosts. as a result of numerous prior studies, we know that host genotypes and abiotic factors influence the composition of plant microbiomes. however, the high complexity of these communities challenges detailed studies to define experimentally the mechanisms underlying the dynamics of community assembly and the beneficial effects of such microbiomes on plant hosts. in this work, from the distinctive microbiota assembled ... | 2017 | 28275097 |
| sequencing and comparative genomics analysis of the inchi2 plasmids pt5282-mpha and p112298-cata and the inchi5 plasmid pynkp001-dfra. | incompatibility group inchi plasmids are important vectors of antibiotic resistance in enterobacteriaceae. in this study, a scheme for typing inchi into five separately clustering subgroups, including previously designated inchi1-3 as well as inchi4-5, was proposed based on sequenced inchi plasmids. the complete nucleotide sequences of the inchi2 plasmids pt5282-mpha and p112298-cata and the inchi5 plasmid pynkp001-dfra from clinical enterobacter cloacae, citrobacter freundii and raoultella orni ... | 2017 | 28390961 |
| multiregional dissemination of kpc-producing klebsiella pneumoniae st258/st512 genotypes in poland, 2010-14. | in 2008-09, the kpc carbapenemase epidemiology in poland was dominated by a klebsiella pneumoniae st258 kpc-2 outbreak in warsaw and its administrative region. the aim of this study was to analyse the situation in 2010-14, with a focus on new outbreaks in other parts of the country. | 2017 | 28333331 |
| global molecular epidemiology of imp-producing enterobacteriaceae. | international data on the molecular epidemiology of enterobacteriaceae with imp carbapenemases are lacking. we performed short-read (illumina) whole-genome sequencing on a global collection of 38 imp-producing clinical enterobacteriaceae (2008 to 2014). imp-producing enterobacteriaceae (7 varieties within 11 class 1 integrons) were mainly present in the south pacific and asia. specific blaimp-containing integrons (in809 with blaimp-4, in722 with blaimp-6, and in687 with blaimp-14) were circulati ... | 2017 | 28167555 |
| interferon-inducible guanylate-binding proteins at the interface of cell-autonomous immunity and inflammasome activation. | guanylate-binding proteins (gbps) are essential components of cell-autonomous immunity. in response to ifn signaling, gbps are expressed in the cytoplasm of immune and nonimmune cells, where they unleash their antimicrobial activity toward intracellular bacteria, viruses, and parasites. recent studies have revealed that gbps are essential for mediating activation of the caspase-1 inflammasome in response to the gram-negative bacteria salmonella enterica serovar typhimurium, francisella novicida, ... | 2017 | 27418355 |
| incn st7 epidemic plasmid carrying blaimp-4 in enterobacteriaceae isolates with epidemiological links to multiple geographical areas in china. | to characterize blaimp-4-carrying plasmids originating from inpatients in hong kong. | 2017 | 27609049 |
| risk of infection transmission in curvilinear array echoendoscopes: results of a prospective reprocessing and culture registry. | the complex design of the elevator mechanism in duodenoscopes has been recognized as a challenge for disinfection and recently implicated as a potential source of persistent bacterial contamination. curvilinear array (cla) echoendoscopes also have an elevator mechanism; however, there are no recommendations or data regarding the risk of persistent bacterial contamination of echoendoscopes. here we hoped to determine the yield of microbial growth with routine bacterial surveillance cultures of re ... | 2017 | 27478029 |
| prevalence and antimicrobial susceptibility of enterobacteriaceae isolated from retail pepper in vietnam. | to investigate the microbial quality of retail pepper in vietnam, the enumeration and detection of enterobacteriaceae and the screening of cefotaxime (ctx)-resistant coliforms were performed by using 84 commercial samples. although enterobacteriaceae were isolated from 78 samples, the number of enterobacteriaceae was lower than 1.0 log cfu/g in 46 samples. for the detection of enterobacteriaceae with the international organization for standardization methods, salmonella spp., escherichia coli , ... | 2017 | 28350183 |
| [etiologic diagnosis in meningitis and encephalitis molecular biology techniques]. | the aetiological study of infections of the central nervous system has traditionally been performed using bacterial cultures and, more recently, using polymerase chain reaction (pcr) for herpes simplex virus (hsv). bacterial cultures may not have good performance, especially in the context of patients who have received antibiotics prior to sampling, and a request for hsv only by pcr reduces the information to only one aetiological agent. the aim of this study is to determine the infectious cause ... | 2017 | 26493774 |
| high-quality genome sequence of human pathogen enterobacter asburiae type strain 1497-78(t). | enterobacter asburiae belongs to the enterobacter cloacae complex (ecc), which comprises six heterogenic species. these bacteria can cause nosocomial infections. moreover, they are well known for antibiotic resistance features based on overproduction of ampc β-lactamases. although ecc have clinical importance, little is known about their virulence-associated properties, and very few strains from the six species have been sequenced. in this study, the type strain of e. asburiae 1497-78(t) (atcc 3 ... | 2017 | 28082144 |
| the draft genome sequence of the plant growth promoting rhizospheric bacterium enterobacter cloacae sbp-8. | here, we report the draft genome sequence and annotation of plant growth promoting rhizobacterium enterobacter cloacae sbp-8 isolated from the rhizosphere of sorghum bicolor l. growing in desert region of rajasthan, india. from the genome sequences, we identified the genes encoding plant growth promoting properties such as 1-aminocyclopropane-1-carboxylate deaminase (acds), phosphate solubilisation, siderophore, and iaa (indole acetic acid) production. the genes encoding different functions requ ... | 2017 | 28386532 |
| draft genome sequence of a high-level colistin-resistant clinical strain of the enterobacter cloacae complex. | strain wchecl-c4 of the enterobacter cloacae complex, recovered from the blood of a patient with peritonitis, was high-level resistant to colistin. here, we report its 5.1-mb draft genome sequence, comprising 92 contigs with an average 55.74% g+c content. the genome contained 4,783 coding sequences and 68 trna genes. | 2017 | 28385849 |
| carbapenemase-producing enterobacteriaceae recovered from a spanish river ecosystem. | the increasing resistance to carbapenems is an alarming threat in the fight against multiresistant bacteria. the dissemination properties of antimicrobial resistance genes are supported by their detection in a diverse population of bacteria, including strains isolated from the environment. the objective of this study was to investigate the presence of carbapenemase-producing enterobacteriaceae (cpe) collected from a river ecosystem in the barcelona metropolitan area (spain). identification of β- ... | 2017 | 28380016 |
| [community acquired urinary tract infections in older adults]. | background community acquired urinary tract infections (cauti) in adults are common ailments. older adults are prone to infectious diseases, diagnosis can be difficult, their etiologic and antimicrobial resistance are poorly known. objectives to evaluate the incidence discriminated by sex, symptoms, and to determine pathogens and their antimicrobial resistance rate. methods a retrospective, descriptive and comparative study. we analyze uricultures among 2013 in the hospital nacional de clinicas ... | 2017 | 28379126 |
| xer site specific recombination: double and single recombinase systems. | the separation and segregation of newly replicated bacterial chromosomes can be constrained by the formation of circular chromosome dimers caused by crossing over during homologous recombination events. in escherichia coli and most bacteria, dimers are resolved to monomers by site-specific recombination, a process performed by two chromosomally encoded tyrosine recombinases (xerc and xerd). xercd recombinases act at a 28 bp recombination site dif, which is located at the replication terminus reg ... | 2017 | 28373867 |
| gallbladder microbiota variability in colombian gallstones patients. | gallbladder stones are a very frequently occurring condition. despite bile bactericidal activity, many bacteria have been detected inside the gallbladder, and gallstones facilitate their presence. between 3% and 5% of the patients with salmonella spp. infection develop the carrier stage, with the bacteria persisting inside the gallbladder, shedding bacteria in their feces without signs of infection. the aim of this study was to isolate bacteria from colombian patients with gallstones, using stan ... | 2017 | 28368860 |
| efficient synthesis of tyrosol galactosides by the β-galactosidase from enterobacter cloacae b5. | in this work, the β-galactosidase from enterobacter cloacae b5 (bgab5) exhibited excellent transglycosylation activity toward tyrosol (p-hydroxyphenethyl alcohol) when using lactose as the glycosyl donor, generating a series of tyrosol glycosides with potential pharmacological properties. the effects of substrate concentration, temperature, ph, and reaction time on the transglycosylation reaction catalyzed by the enzyme bgab5 were studied in detail. three tyrosol derivatives were produced in a t ... | 2017 | 28361236 |
| troubled water under the bridge: screening of river mur water reveals dominance of ctx-m harboring escherichia coli and for the first time an environmental vim-1 producer in austria. | antibiotic resistant bacteria (arb) in the aquatic environment are reported from all over the world and their presence in the environment has become quite common. the current most prominent example is the presence of beta-lactamases harboring enterobacteriaceae. the aim of this study was to investigate the presence and diversity (on the genetic and phenotypic levels) of extended spectrum beta-lactamases (esbl) and carbapenemases harboring enterobacteriaceae from the river mur in the center of gr ... | 2017 | 28351808 |
| structural and genetic characterization of the o-antigen of enterobacter cloacae c5529 related to the o-antigen of e. cloacae g3054. | on mild acid degradation of the lipopolysaccharide of enterobacter cloacae c5529, the o-polysaccharide chain was cleaved at the linkages of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-n-acetylpseudaminic acid, psep5ac7ac). the resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)h and (13)c nmr spectroscopy, and the following structure of the tetrasaccharide repeating unit of the o-polysaccharide was esta ... | 2017 | 28342969 |
| two-year follow-up outcomes of premature infants enrolled in the phase i trial of mesenchymal stem cells transplantation for bronchopulmonary dysplasia. | to determine the long-term safety and outcomes of mesenchymal stem cells (mscs) for bronchopulmonary dysplasia in premature infants enrolled in a previous phase i clinical trial up to 2 years of corrected age (ca). | 2017 | 28341525 |
| high prevalence of non-clonal imipenem-nonsusceptible enterobacter spp. isolates in korea and their association with porin down-regulation. | we investigated the prevalence and clonal distribution of imipenem-nonsusceptible enterobacter clinical isolates from hospitals in korea and the contributions of various mechanisms to imipenem nonsusceptibility. the in vitro antimicrobial susceptibility to imipenem of 357 non-duplicated enterobacter isolates obtained from eight geographically distant tertiary care hospitals in korea was evaluated. imipenem-nonsusceptible enterobacter isolates were genotyped. additionally, β-lactamase genes were ... | 2017 | 28336135 |
| soft tissue infections from fish spike wounds: normal commensal bacteria are more common than marine pathogens. | a fish spike injury can be sustained by anyone handling fish; during fishing, meal preparation or in retail. case reports of fish spikes inoculating victims with virulent marine-specific pathogens and causing systemic illness led us to question whether empirical treatment of these injuries with amoxicillin and clavulanic acid is adequate. | 2017 | 28320052 |
| screening for fecal carriage of mcr-producing enterobacteriaceae in healthy humans and primary care patients. | the extent of the occurrence of the plasmid-encoded colistin resistance genes mcr-1 and mcr-2 among humans is currently sparsely studied in western europe. | 2017 | 28316780 |
| ulcerative granuloma of the eyelid as the initial manifestation of granulomatosis with polyangiitis (wegener's granulomatosis): a case report. | a 56-year-old-man presented a 2-month history of chalazion in the eyelids without response to treatment and with an inconclusive biopsy. laboratory results confirmed the presence of enterobacter cloacae and streptococcus gordonii infection. despite appropriate intravenous antibiotic treatment, clinical worsening was observed. radical surgical excision and total tarsorrhaphy were performed. following treatment, the patient was asymptomatic for 6 weeks until he developed acute renal failure, gener ... | 2017 | 28306365 |
| in vitro activity of imipenem against carbapenemase-positive enterobacteriaceae: smart global surveillance program 2008-2014. | the study for monitoring antimicrobial resistance trends (smart) global surveillance program collected 103,960 isolates of enterobacteriaceae from 2008 to 2014. from this isolate collection all ertapenem-non-susceptible isolates (mic, ≥1 μg/ml; n = 3,428) and 9,371 isolates of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, and proteus mirabilis with an ertapenem-susceptible extended-spectrum β-lactamase (esbl)-positive phenotype were assessed for the presence of common carbapenemas ... | 2017 | 28298454 |
| does second place count? lessons from a major discrepancy between carbapenem-resistant klebsiella pneumoniae and carbapenem-resistant enterobacter cloacae in a one-year follow-up study. | 2017 | 28260534 | |
| in vitro activity of ceftaroline and comparator agents against gram-positive and gram-negative clinical isolates from cancer patients. | bacterial infections are common in cancer patients. ceftaroline (cft) is a broad-spectrum cephalosporin with activity against most gram-positive organisms (gpos) and many gram-negative organisms. in this study, the in vitro activity of cft was compared with vancomycin (van), daptomycin (dap), linezolid (lzd), trimethoprim/sulphamethoxazole (sxt) and tigecycline (tig) against bacteria (predominantly blood culture isolates) isolated from cancer patients in 2014 and 2015. cft was active against met ... | 2017 | 28257904 |
| human norovirus binding to select bacteria representative of the human gut microbiota. | recent reports describe the ability of select bacterial strains to bind human norovirus, although the specificity of such interactions is unknown. the purpose of this work was to determine if a select group of bacterial species representative of human gut microbiota bind to human norovirus, and if so, to characterize the intensity and location of that binding. the bacteria screened included naturally occurring strains isolated from human stool (klebsiella spp., citrobacter spp., bacillus spp., e ... | 2017 | 28257478 |
| very high prevalence of extended-spectrum beta-lactamase-producing enterobacteriaceae in bacteriemic patients hospitalized in teaching hospitals in bamako, mali. | the worldwide dissemination of extended-spectrum beta-lactamase producing enterobacteriaceae, (esbl-e) and their subset producing carbapenemases (cpe), is alarming. limited data on the prevalence of such strains in infections from patients from sub-saharan africa are currently available. we determined, here, the prevalence of esbl-e/cpe in bacteriemic patients in two teaching hospitals from bamako (mali), which are at the top of the health care pyramid in the country. during one year, all entero ... | 2017 | 28245252 |
| aerobic and heterotrophic nitrogen removal by enterobacter cloacae cf-s27 with efficient utilization of hydroxylamine. | heterotrophic bacterium, enterobacter cloacae cf-s27 exhibited simultaneous nitrification and aerobic denitrification in presence of high concentration of hydroxylamine. with the initial nitrogen concentration of 100mgl(-1)h(-1), ammonium, nitrate and nitrite removal efficiencies were 81%, 99.9% and 92.8%, while the corresponding maximum removal rates reached as high as 11.6, 15.1 and 11.2mgl(-1)h(-1) respectively. quantitative amplification by real time pcr and enzyme assay demonstrated that hy ... | 2017 | 28242385 |
| enterobacter cloacae complex isolates harboring blanmc-a or blaimi-type class a carbapenemase genes on novel chromosomal integrative elements and plasmids. | carbapenem-resistant enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by pcr for seven common carbapenemase gene groups, namely, kpc, ndm, oxa-48, vim, imp, ges, and nmc-a/imi. nineteen of the submitted isolates (1.7%) were found to harbor ambler class a blanmc-a or blaimi-type carbapenemases. all 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprof ... | 2017 | 28223374 |
| the locus of heat resistance (lhr) mediates heat resistance in salmonella enterica, escherichia coli and enterobacter cloacae. | enterobacteriaceae comprise food spoilage organisms as well as food-borne pathogens including escherichia coli. heat resistance in e. coli was attributed to a genomic island called the locus of heat resistance (lhr). this genomic island is also present in several other genera of enterobacteriaceae, but its function in the enteric pathogens salmonella enterica and enterobacter cloacae is unknown. this study aimed to determine the frequency of the lhr in food isolates of e. coli, and its influence ... | 2017 | 28213040 |
| the overproduction of indole-3-acetic acid (iaa) in endophytes upregulates nitrogen fixation in both bacterial cultures and inoculated rice plants. | endophytic bacteria from roots and leaves of rice plants were isolated and identified in order to select the diazotrophs and improve their nitrogen-fixing abilities. the nitrogen-fixing endophytes were identified by pcr amplification of the nifh gene fragment. for this purpose, two isolates, enterobacter cloacae rca25 and klebsiella variicola rca26, and two model bacteria (herbaspirillum seropedicae z67 and sinorhizobium fredii ngr234) were transformed to increase the biosynthesis of the main pl ... | 2017 | 28197647 |
| molecular characteristics of carbapenem-resistant enterobacter cloacae in ningxia province, china. | the emergence of carbapenem-resistant enterobacteriaceae (cre) has become a major public health concern worldwide and a new challenge in the treatment of infectious diseases. the molecular characteristics of enterobacter cloacae in ningxia china are unknown. in this study, we reported 10 carbapenem-resistant e. cloacae isolates from the general hospital of ningxia medical university, the largest university hospital in ningxia between january 2012 and december 2013. bacteria isolates were identif ... | 2017 | 28197140 |
| molecular epidemiology of carbapenemase-producing enterobacteriaceae in a primary care hospital in japan, 2010-2013. | recently, carbapenemase-producing enterobacteriaceae (cpe) have been spreading worldwide and have become a threat in healthcare systems. we investigated the isolation frequency and molecular epidemiological characteristics of cpe isolated from clinical samples collected at a primary care hospital over the four years of 2010-2013 in japan. cpe were detected in 17 (0.34%) of 4875 isolates by the broth microdilution method, sodium mercaptoacetate inhibition test, and modified hodge test using merop ... | 2017 | 28161293 |
| potency and selectivity indices of acetone leaf extracts of nine selected south african trees against six opportunistic enterobacteriaceae isolates from commercial chicken eggs. | the rise in antimicrobial resistance in a plethora of nosocomial and opportunistic bacterial pathogens often isolated from commercial eggs, poses a serious public health concern. the existence of these contaminants may also serve as a drawback in the current efforts of improving the well-being of immunocompromised patients. the aim of this study was to determine the efficacy of plant extracts that had good activity on escherichia coli in previous word on pathogens isolated from eggs for possible ... | 2017 | 28148263 |
| enantio convergent biotransformation of o,o-dimethyl-4-oxoazetidin-2-ylphosphonate using fungal cells of penicillium minioluteum and purified enzymes. | this report presents the bioconversion of o,o-dimethyl-4-oxoazetidin-2-ylphosphonate 1 performed in two ways: with the enzymatic system of p. minioluteum and with the application of purified enzymes: penicillinase and two proteases of different origin. recorded nmr spectra allowed confirming the reaction progress and also postulating possible mechanism of conversion. the path of bioconversion was defined as enantio convergent process for both modes of applied biocatalysts. this means that kineti ... | 2017 | 28143659 |
| prevalence, risk factors, outcomes, and molecular epidemiology of mcr-1-positive enterobacteriaceae in patients and healthy adults from china: an epidemiological and clinical study. | the mcr-1 gene confers transferable colistin resistance. mcr-1-positive enterobacteriaceae (mcrpe) have attracted substantial medical, media, and political attention; however, so far studies have not addressed their clinical impact. herein, we report the prevalence of mcrpe in human infections and carriage, clinical associations of mcr-1-positive escherichia coli (mcrpec) infection, and risk factors for mcrpec carriage. | 2017 | 28139431 |
| [profile of bacterial resistance in pediatric urinary tract infections in 2014]. | in pediatric units, bacteria-producing extended-spectrum-betalactamase (esbl) have an increasing prevalence among bacteria causing febrile urinary tract infections (utis). the purpose of this study was to evaluate the epidemiology of bacteria resistance patterns observed in utis, in order to assess the current antibiotic treatment protocols. this study is based upon a single-center retrospective chart review of the cytobacteriological urine cultures performed in utis between 1 january and 31 dec ... | 2017 | 28131557 |
| effect of preoperative biliary drainage on bacterial flora in bile of patients with periampullary cancer. | patients with obstructive jaundice due to periampullary tumours may undergo preoperative biliary drainage (pbd). the effect of pbd on the microbiome of the biliary system and on postoperative outcome remains unclear. | 2017 | 28121036 |
| optimization of enterobacter cloacae (ku923381) for diesel oil degradation using response surface methodology (rsm). | efficiency of enterobacter cloacae ku923381 isolated from petroleum hydrocarbon contaminated soil was evaluated in batch culture and bioreactor mode. the isolate were screened for biofilm formation using qualitative and quantitative assays. response surface methodology (rsm) was used to study the effect of ph, temperature, glucose concentration, and sodium chloride on diesel degradation. the predicted values for diesel oil degradation efficiency by the statistical designs are in a close agreemen ... | 2017 | 28120192 |
| bioadsorption and biostabilization of cadmium by enterobacter cloacae tu. | biostabilization of cadmium, a hazardous chemical found widely in china, was attempted using enterobacter cloacae tu (e.cloacae tu). a cadmium (cd)-tolerant e.cloacae tu was obtained by mutagenesis using an atmosphere pressure glow discharge plasma system, and it displayed regular growth behavior in the presence of 250 mg/l cd in solution. the maximum stabilization capacity of e.cloacae tu toward cd reached 67.0 ± 3.5 mg/g dry cell weight at an initial cd concentration of 200 mg/l. the percentag ... | 2017 | 28117094 |
| in vitro susceptibility to 19 agents other than β-lactams among third-generation cephalosporin-resistant enterobacteriaceae recovered on hospital admission. | as part of the multicentre antibiotic therapy optimisation study, mic values of 19 non-β-lactam agents were determined for third-generation cephalosporin-resistant escherichia coli, klebsiella species and enterobacter species (3gcreb) isolates collected in german hospitals. | 2017 | 28108677 |
| emergence of blandm-7-producing enterobacteriaceae in gabon, 2016. | reports of carbapenemase-producing enterobacteriaceae in africa remain rare and assess mostly blaoxa-48-producing isolates from mediterranean countries and south africa. we identified blandm-7-producing enterobacteriaceae in gabon in 2016. the isolates contained blandm-7 incx3 plasmids that were unusual and similar to the one described in a colistin-resistant klebsiella pneumoniae sz04 isolate from china. | 2017 | 28098536 |
| in vitro activity of ceftazidime/avibactam against urinary isolates from patients in a phase 3 clinical trial programme for the treatment of complicated urinary tract infections. | to evaluate the in vitro activity of ceftazidime/avibactam relative to comparator agents against gram-negative isolates from a phase 3 clinical trial programme for complicated urinary tract infections (recapture). | 2017 | 28088768 |
| antimicrobial activity of ceftazidime-avibactam and comparator agents when tested against bacterial isolates causing infection in cancer patients (2013-2014). | we evaluated the antimicrobial susceptibility of 623 gram-negative organisms causing infection in patients with cancer in 52 united states hospitals (2013-2014) as part of the international network for optimal resistance monitoring (inform) program. isolates were tested for susceptibility by broth microdilution method. β-lactamase encoding genes were evaluated for all escherichia coli and klebsiella spp. with an extended-spectrum β-lactamase (esbl) phenotype by microarray-based assay. esbl-pheno ... | 2017 | 28069328 |
| the pharmacodynamics of avibactam in combination with ceftaroline or ceftazidime against β-lactamase-producing enterobacteriaceae studied in an in vitro model of infection. | pharmacodynamics of β-lactamase inhibitors are an area of intense interest as new β-lactam/β-lactamase inhibitor combinations enter clinical development and clinical practice. avibactam, a non-β-lactam β-lactamase inhibitor, has been combined with ceftaroline or ceftazidime but these two combinations have not been directly compared. | 2017 | 28039276 |
| antibiofilm efficacy of green synthesized graphene oxide-silver nanocomposite using lagerstroemia speciosa floral extract: a comparative study on inhibition of gram-positive and gram-negative biofilms. | biofilm architecture provides bacteria with enhanced antibiotic resistance, thus raising the need to search for alternative therapies that can inhibit the bacterial colonization. in the present study, we synthesized graphene oxide-silver nanocomposite (go-ag) by non-toxic and eco-friendly route using a floral extract of legistromia speciosa (l.) pers. the gas chromatography-mass spectrometry (gc-ms) analysis of plant extract revealed the presence of compounds which can simultaneously act as redu ... | 2017 | 28027944 |
| [epidemiology and risk factors of patients with intra-abdominal postsurgical infection treated with tigecycline: a cohort study]. | to study a cohort of patients with intra-abdominal postsurgical infection treated with tigecycline to analyze its effectiveness and mortality related factors. | 2017 | 28010058 |
| corrigendum to "carbapenem susceptibility among escherichia coli, klebsiella pneumoniae, and enterobacter cloacae isolates obtained from patients in intensive care units in taiwan in 2005, 2007, and 2009" [diagn microbiol infect dis 2015;81(4):290-295]. | 2017 | 27993423 | |
| structural insight into mode of binding of meropenem to ctx-m-15 type β-lactamase. | among enterobacteriaceae, ctx-m type extended spectrum beta lactamase confers potent hydrolytic activity against cephalosporin group of antibiotics. strains producing ctx-m type beta lactamase enzymes, show high level of resistance against cefotaxime. therefore carbapenem antibiotics are used against beta lactamase producing strains. hence, this study was designed to understand an insight of molecular basis of ctx-m-15 interaction with meropenem, and its effect on ctx-m-15 efficiency. clinical s ... | 2017 | 27986632 |
| reduction of quinones and nitroaromatic compounds by escherichia coli nitroreductase a (nfsa): characterization of kinetics and substrate specificity. | nfsa, a major fmn-associated nitroreductase of e. coli, reduces nitroaromatic compounds via consecutive two-electron transfers. nfsa has potential applications in the biodegradation of nitroaromatic environment pollutants, e.g. explosives, and is also of interest for the anticancer strategy gene-directed enzyme prodrug therapy. however, the catalytic mechanism of nfsa is poorly characterized. here we examined the nadph-dependent reduction of quinones (n = 16) and nitroaromatic compounds (n = 12) ... | 2017 | 27986535 |
| molecular and in silico analysis of a new plasmid-mediated ampc β-lactamase (cmh-2) in clinical isolates of klebsiella pneumoniae. | two klebsiella strains isolated from urine samples were positive for blaampc by pcr and showed sequence similarity with cmh-1 (98.6%) after sequencing. it also shares 82% similarity with act-1, 85% with mir-1 and 81% with the chromosomal ampc gene of enterobacter cloacae. this gene was associated with the plasmid of inck type. it has an open reading frame of 381 amino acid with four amino acid substitutions at position d144a, c189r, q192e, and a195t as compared to cmh-1. when expressed in e.coli ... | 2017 | 27964935 |
| inventory of extended-spectrum-β-lactamase-producing enterobacteriaceae in france as assessed by a multicenter study. | the objective of this study was to perform an inventory of the extended-spectrum-β-lactamase (esbl)-producing enterobacteriaceae isolates responsible for infections in french hospitals and to assess the mechanisms associated with esbl diffusion. a total of 200 nonredundant esbl-producing enterobacteriaceae strains isolated from clinical samples were collected during a multicenter study performed in 18 representative french hospitals. antibiotic resistance genes were identified by pcr and sequenc ... | 2017 | 27956424 |
| intense pre-admission carriage and further acquisition of esbl-producing enterobacteriaceae among patients and their caregivers in a tertiary hospital in rwanda. | to assess the presence and risk factors of intestinal carriage of extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-pe) among patients admitted to the university teaching hospital of butare and among their attending caregivers, and to analyse the acquisition of esbl-pe carriage during hospital stay and associated factors. | 2017 | 27935649 |
| searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds. | the aims of this study were to design, synthesize and to evaluate 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against gram-negative and gram-positive bacterial strains, including methicillin-resistant staphylococcus aureus (mrsa) and its biofilm, to probe for potential lead structures. | 2017 | 27930849 |
| a prolonged outbreak of kpc-3-producing enterobacter cloacae and klebsiella pneumoniae driven by multiple mechanisms of resistance transmission at a large academic burn center. | klebsiella pneumoniae carbapenemase (kpc)-producing enterobacter cloacae has been recently recognized in the united states. whole-genome sequencing (wgs) has become a useful tool for analysis of outbreaks and for determining transmission networks of multidrug-resistant organisms in health care settings, including carbapenem-resistant enterobacteriaceae (cre). we experienced a prolonged outbreak of cre e. cloacae and k. pneumoniae over a 3-year period at a large academic burn center despite rigor ... | 2017 | 27919898 |
| clonal spread and accumulation of β-lactam resistance determinants in enterobacter aerogenes and enterobacter cloacae complex isolates from infection and colonization in patients at a public hospital in recife, pernambuco, brazil. | enterobacter aerogenes and enterobacter cloacae complex are the two species of this genus most involved in healthcare-associated infections that are esbl and carbapenemase producers. this study characterized, phenotypically and genotypically, 51 isolates of e. aerogenes and e. cloacae complex originating from infection or colonization in patients admitted to a public hospital in recife, pernambuco, brazil, by antimicrobial susceptibility profile, analysis of β-lactamase genes (blatem, blashv, bl ... | 2017 | 27902398 |
| [evaluation of an immunochromatographic test for the detection of oxa-48 carbapenemase]. | detection and differentiation of various types of carbapenemases is crucial to their control and dissemination. oxa-48 is the most common carbapenemase in spain and in our environment. the aim of this study is the evaluation of a new immunochromatographic test oxa-48 card letitest (coris, bioconcept belgium) to detect this carbapenemase from solid media. | 2017 | 27897435 |
| two symbiotic bacteria of the entomopathogenic nematode heterorhabditis spp. against galleria mellonella. | the entomopathogenic nematode heterorhabditis spp. is considered a promising agent in the biocontrol of injurious insects of agriculture. however, different symbiotic bacteria associated with the nematode usually have different specificity and virulence toward their own host. in this study, two symbiotic bacteria, ly2w and nk, were isolated from the intestinal canals of two entomopathogenic nematode heterorhabditis megidis 90 (pdsj1 and pdsj2) from galleria mellonela, separately. to determine th ... | 2017 | 27889603 |
| molecular characterization of oxa-48-like-producing enterobacteriaceae in the czech republic and evidence for horizontal transfer of poxa-48-like plasmids. | the aim of this study was to characterize the first cases and outbreaks of oxa-48-like-producing enterobacteriaceae recovered from hospital settings in the czech republic. from 2013 to 2015, 22 klebsiella pneumoniae isolates, 3 escherichia coli isolates, and 1 enterobacter cloacae isolate producing oxa-48-like carbapenemases were isolated from 20 patients. four of the patients were colonized or infected by two or three different oxa-48-like producers. the k. pneumoniae isolates were classified i ... | 2017 | 27855076 |
| distribution of esbls, ampc β-lactamases and carbapenemases among enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the asia-pacific region during 2008-14: results from the study for monitoring antimicrobial resistance trends (smart). | to investigate the antimicrobial resistance and assess the molecular characteristics of β-lactamases (esbls, ampc β-lactamases and carbapenemases) among enterobacteriaceae isolates that caused intra-abdominal infections (iais) in patients hospitalized in the asia-pacific region during 2008-14. | 2017 | 27703058 |
| early cerebral abscesses secondary to enterobacter cloacae sepsis in an extreme preterm. | 2017 | 27566668 |