Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| comparative investigation on a hexane-degrading strain with different cell surface hydrophobicities mediated by starch and chitosan. | bioremediation usually exhibits low removal efficiency toward hexane because of poor water solubility, which limits the mass transfer rate between the substrate and microorganism. this work aimed to enhance the hexane degradation rate by increasing cell surface hydrophobicity (csh) of the degrader, pseudomonas mendocina nx-1. the csh of p. mendocina nx-1 was manipulated by treatment with starch and chitosan solution of varied concentrations, reaching a maximum hydrophobicity of 52%. the biodegra ... | 2017 | 28091789 |
| diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in liangshui river of beijing, china. | aerobic denitrification is a process reducing the nitrate into gaseous nitrogen forms in the presence of oxygen gas, which makes the nitrification and denitrification performed simultaneously. however, little was known on the diversity of the culturable aerobic denitrifying bacteria in the surface water system. in this study, 116 strains of aerobic denitrifying bacteria were isolated from the sediment, water and biofilm samples in liangshui river of beijing. these bacteria were classified into 1 ... | 2017 | 28855587 |
| phenotypic and genomic survey on organic acid utilization profile of pseudomonas mendocina strain s5.2, a vineyard soil isolate. | root exudates are chemical compounds that are released from living plant roots and provide significant energy, carbon, nitrogen and phosphorus sources for microbes inhabiting the rhizosphere. the exudates shape the microflora associated with the plant, as well as influences the plant health and productivity. therefore, a better understanding of the trophic link that is established between the plant and the associated bacteria is necessary. in this study, a comprehensive survey on the utilization ... | 2017 | 28655216 |
| biodegradation of p(3hb-co-4hb) powder by pseudomonas mendocina for preparation low-molecular-mass p(3hb-co-4hb). | poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (p(3hb-co-4hb)) is a biodegradable plastic that is extensively utilized in many fields. in this work, p(3hb-co-4hb) powder was degraded by pseudomonas mendocina for the preparation of low-molecular-mass (lmw) p(3hb-co-4hb). after degradation, the remaining p(3hb-co-4hb) powder was analyzed via gel permeation chromatography (gpc), differential scanning calorimetry (dsc), x-ray powder diffraction (xrd), fourier transform infrared (ftir) spectroscopy, an ... | 2017 | 28828288 |
| degradation and conversion of endosulfan by newly isolated pseudomonas mendocina zam1 strain. | endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. therefore, the aim of this study was to isolate and identify a novel bacterial strain capable of degrading endosulfan in agriculture contaminated soils. a novel bacterial strain was isolated from the sugarcane field contaminated with endosulfan, and was named as zam1 strain. the zam1 bacterial strain was further identified as pseudomonas men ... | 2017 | 28667651 |
| pseudomonas mendocina: the first case of peritonitis on peritoneal dialysis. | 2017 | 28655401 | |
| enhancement of medium-chain-length polyhydroxyalkanoates biosynthesis from glucose by metabolic engineering in pseudomonas mendocina. | to enhance the biosynthesis of medium-chain-length polyhydroxyalkanoates (phamcl) from glucose in pseudomonas mendocina nk-01, metabolic engineering strategies were used to block or enhance related pathways. | 2016 | 26476529 |
| isolation and characterization of bacteria that use furans as the sole carbon source. | five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. based on 16s rrna phylogenetic analysis, these strains were identified as cupriavidus pinatubonensis (designated als1280), pigmentiphaga sp. (als1172), pseudomonas sp. bwdy (als1279), pseudomonas mendocina (als1131), and pseudomonas putida (als1267). in all cases, growth under oxygenated conditions on furfural was accompanied by the transient accumulation of 2-furo ... | 2016 | 26419660 |
| finished genome sequence and methylome of the cyanide-degrading pseudomonas pseudoalcaligenes strain cect5344 as resolved by single-molecule real-time sequencing. | pseudomonas pseudoalcaligenes cect5344 tolerates cyanide and is also able to utilize cyanide and cyano-derivatives as a nitrogen source under alkaline conditions. the strain is considered as candidate for bioremediation of habitats contaminated with cyanide-containing liquid wastes. information on the genome sequence of the strain cect5344 became available previously. the p. pseudoalcaligenes cect5344 genome was now resequenced by applying the single molecule, real-time (smrt(®)) sequencing tech ... | 2016 | 27060556 |
| efficient production of polyhydroxyalkanoates (phas) from pseudomonas mendocina psu using a biodiesel liquid waste (blw) as the sole carbon source. | conditions for the optimal production of polyhydroxyalkanoate (pha) by pseudomonas mendocina psu using a biodiesel liquid waste (blw) were determined by response surface methodology. these were an initial carbon to nitrogen ratio (c/n) of 40 (mole/mole), an initial ph of 7.0, and a temperature of 35 °c. a biomass and pha concentration of 3.65 g/l and about 2.6 g/l (77% dcw), respectively, were achieved in a growth associated process using 20 g/l glycerol in the blw after 36 h of exponential grow ... | 2016 | 26981955 |
| the isolation and functional identification on producing cellulase of pseudomonas mendocina. | the straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of n,p,k and other effective elements in ecological system. we isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in northeast of china, which can produce cellulase efficiently. the strain was identified as pseudomonas mendocina by using morphological, physiological, biochemical test, and molecular b ... | 2016 | 27710430 |
| t4 lysozyme fused with cellulose-binding module for antimicrobial cellulosic wound dressing materials. | bacterial infection is a major challenge in wound care. antimicrobial wound dressings are of great value for treating wound infections. endolysins are evolving as a new class of antimicrobials with multiple applications. this study describes the production and evaluation of t4 lysozyme (t4lyz), product of gene e of the t4 bacteriophage, fused with cellulose binding module (cbm) for facile immobilization to cellulosic wound dressing. | 2016 | 27028513 |
| molecular insights into toluene sensing in the tods/todt signal transduction system. | tods is a sensor kinase that responds to various monoaromatic compounds, which either cause an agonistic or antagonistic effect on phosphorylation of its cognate response regulator todt, and controls tod operon expression in pseudomonas putida strains. we describe a molecular sensing mechanism of tods that is activated in response to toluene. the crystal structures of the tods per-arnt-sim (pas) 1 sensor domain (residues 43-164) and its complex with toluene (agonist) or 1,2,4-trimethylbenzene (a ... | 2016 | 26903514 |
| protein sequence annotation tool (psat): a centralized web-based meta-server for high-throughput sequence annotations. | here we introduce the protein sequence annotation tool (psat), a web-based, sequence annotation meta-server for performing integrated, high-throughput, genome-wide sequence analyses. our goals in building psat were to (1) create an extensible platform for integration of multiple sequence-based bioinformatics tools, (2) enable functional annotations and enzyme predictions over large input protein fasta data sets, and (3) provide a web interface for convenient execution of the tools. | 2016 | 26792120 |
| all the microbiology nematodes can teach us. | be it their pervasiveness, experimental tractability or their impact on human health and agriculture, nematode-bacterium associations are far-reaching research subjects. although the omics hype did not spare them and helped reveal mechanisms of communication and exchange between the associated partners, a huge amount of knowledge still awaits to be harvested from their study. here, i summarize and compare the kind of research that has been already performed on the model nematode caenorhabditis e ... | 2016 | 26839382 |
| direct identification of urinary tract pathogens from urine samples, combining urine screening methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry. | early diagnosis of urinary tract infections (utis) is essential to avoid inadequate or unnecessary empirical antibiotic therapy. microbiological confirmation takes 24 to 48 h. the use of screening methods, such as cytometry and automated microscopic analysis of urine sediment, allows the rapid prediction of negative samples. in addition, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is a widely established technique in clinical microbiology laborator ... | 2016 | 26818668 |
| the diguanylate cyclase hsbd intersects with the hptb regulatory cascade to control pseudomonas aeruginosa biofilm and motility. | the molecular basis of second messenger signaling relies on an array of proteins that synthesize, degrade or bind the molecule to produce coherent functional outputs. cyclic di-gmp (c-di-gmp) has emerged as a eubacterial nucleotide second messenger regulating a plethora of key behaviors, like the transition from planktonic cells to biofilm communities. the striking multiplicity of c-di-gmp control modules and regulated cellular functions raised the question of signaling specificity. are c-di-gmp ... | 2016 | 27792789 |
| host-specific functional significance of caenorhabditis gut commensals. | the gut microbiota is an important contributor to host health and fitness. given its importance, microbiota composition should not be left to chance. however, what determines this composition is far from clear, with results supporting contributions of both environmental factors and host genetics. to gauge the relative contributions of host genetics and environment, specifically the microbial diversity, we characterized the gut microbiotas of caenorhabditis species spanning 200-300 million years ... | 2016 | 27799924 |
| pseudomonas mendocina native valve infective endocarditis: a case report. | gram-negative microorganisms are uncommon pathogens responsible for infective endocarditis. pseudomonas mendocina, a gram-negative water-borne and soil-borne bacterium, was first reported to cause human infection in 1992. since then, it has rarely been reported as a human pathogen in the literature. we describe the first case of native valve infective endocarditis due to p. mendocina in the usa. | 2016 | 27716406 |
| biodegradation of methyl tert-butyl ether by co-metabolism with a pseudomonas sp. strain. | co-metabolic bioremediation is supposed to be an impressive and promising approach in the elimination technology of methyl tert-butyl ether (mtbe), which was found to be a common pollutant worldwide in the ground or underground water in recent years. in this paper, bacterial strain dz13 (which can co-metabolically degrade mtbe) was isolated and named as pseudomonas sp. dz13 based on the result of 16s rrna gene sequencing analysis. strain dz13 could grow on n-alkanes (c₅-c₈), accompanied with the ... | 2016 | 27608032 |
| the native microbiome of the nematode caenorhabditis elegans: gateway to a new host-microbiome model. | host-microbe associations underlie many key processes of host development, immunity, and life history. yet, none of the current research on the central model species caenorhabditis elegans considers the worm's natural microbiome. instead, almost all laboratories exclusively use the canonical strain n2 and derived mutants, maintained through routine bleach sterilization in monoxenic cultures with an e. coli strain as food. here, we characterize for the first time the native microbiome of c. elega ... | 2016 | 27160191 |
| siderophore-mediated iron dissolution from nontronites is controlled by mineral cristallochemistry. | bacteria living in oxic environments experience iron deficiency due to limited solubility and slow dissolution kinetics of iron-bearing minerals. to cope with iron deprivation, aerobic bacteria have evolved various strategies, including release of siderophores or other organic acids that scavenge external fe(iii) and deliver it to the cells. this research investigated the role of siderophores produced by pseudomonas aeruginosa in the acquisition of fe(iii) from two iron-bearing colloidal nontron ... | 2016 | 27064911 |
| it all starts with a sandwich: identification of sialidases with trans-glycosylation activity. | sialidases (3.2.1.18) may exhibit trans-sialidase activity to catalyze sialylation of lactose if the active site topology is congruent with that of the trypanosoma cruzi trans-sialidase (ec 2.4.1.-). the present work was undertaken to test the hypothesis that a particular aromatic sandwich structure of two amino acids proximal to the active site of the t. cruzi trans-sialidase infers trans-sialidase activity. on this basis, four enzymes with putative trans-sialidase activity were identified thro ... | 2016 | 27367145 |
| the toxin-antitoxin system dartg catalyzes reversible adp-ribosylation of dna. | the discovery and study of toxin-antitoxin (ta) systems helps us advance our understanding of the strategies prokaryotes employ to regulate cellular processes related to the general stress response, such as defense against phages, growth control, biofilm formation, persistence, and programmed cell death. here we identify and characterize a ta system found in various bacteria, including the global pathogen mycobacterium tuberculosis. the toxin of the system (dart) is a domain of unknown function ... | 2016 | 27939941 |
| mechanistic insights into c-di-gmp-dependent control of the biofilm regulator fleq from pseudomonas aeruginosa. | bacterial biofilm formation during chronic infections confers increased fitness, antibiotic tolerance, and cytotoxicity. in many pathogens, the transition from a planktonic lifestyle to collaborative, sessile biofilms represents a regulated process orchestrated by the intracellular second-messenger c-di-gmp. a main effector for c-di-gmp signaling in the opportunistic pathogen pseudomonas aeruginosa is the transcription regulator fleq. fleq is a bacterial enhancer-binding protein (bebp) with a ce ... | 2016 | 26712005 |
| mechanistic insights into c-di-gmp-dependent control of the biofilm regulator fleq from pseudomonas aeruginosa. | bacterial biofilm formation during chronic infections confers increased fitness, antibiotic tolerance, and cytotoxicity. in many pathogens, the transition from a planktonic lifestyle to collaborative, sessile biofilms represents a regulated process orchestrated by the intracellular second-messenger c-di-gmp. a main effector for c-di-gmp signaling in the opportunistic pathogen pseudomonas aeruginosa is the transcription regulator fleq. fleq is a bacterial enhancer-binding protein (bebp) with a ce ... | 2016 | 26712005 |
| novel m4c modification in type i restriction-modification systems. | we identify a new subgroup of type i restriction-modification enzymes that modify cytosine in one dna strand and adenine in the opposite strand for host protection. recognition specificity has been determined for ten systems using smrt sequencing and each recognizes a novel dna sequence motif. previously characterized type i systems use two identical copies of a single methyltransferase (mtase) subunit, with one bound at each half site of the specificity (s) subunit to form the mtase. the new m4 ... | 2016 | 27580720 |
| molecular cloning, heterologous expression, and enzymatic characterization of lysoplasmalogen-specific phospholipase d from thermocrispum sp. | lysoplasmalogen (lypls)-specific phospholipase d (lypls-pld) is an enzyme that catalyses the hydrolytic cleavage of the phosphoester bond of lypls, releasing ethanolamine or choline, and 1-(1-alkenyl)-sn-glycero-3-phosphate (lysoplasmenic acid). little is known about lypls-pld and metabolic pathways of plasmalogen (pls). reportedly, pls levels in human serum/plasma correlate with several diseases such as alzheimer's disease and arteriosclerosis as well as a variety of biological processes includ ... | 2016 | 27833852 |
| impact of soil salinity on the structure of the bacterial endophytic community identified from the roots of caliph medic (medicago truncatula). | in addition to being a forage crop, caliph medic (medicago truncatula) is also a model legume plant and is used for research focusing on the molecular characterization of the interaction between rhizobia and plants. however, the endophytic microbiome in this plant is poorly defined. endophytic bacteria play a role in supplying plants with the basic requirements necessary for growth and development. moreover, these bacteria also play a role in the mechanism of salinity stress adaptation in plants ... | 2016 | 27391592 |
| extremophiles in an antarctic marine ecosystem. | recent attempts to explore marine microbial diversity and the global marine microbiome have indicated a large proportion of previously unknown diversity. however, sequencing alone does not tell the whole story, as it relies heavily upon information that is already contained within sequence databases. in addition, microorganisms have been shown to present small-to-large scale biogeographical patterns worldwide, potentially making regional combinations of selection pressures unique. here, we focus ... | 2016 | 27681902 |
| potential of biological agents in decontamination of agricultural soil. | pesticides are widely used for the control of weeds, diseases, and pests of cultivated plants all over the world, mainly since the period after the second world war. the use of pesticides is very extensive to control harm of pests all over the globe. persistent nature of most of the synthetic pesticides causes serious environmental concerns. decontamination of these hazardous chemicals is very essential. this review paper elaborates the potential of various biological agents in decontamination o ... | 2016 | 27293964 |
| screen of non-annotated small secreted proteins of pseudomonas syringae reveals a virulence factor that inhibits tomato immune proteases. | pseudomonas syringae pv. tomato dc3000 (ptodc3000) is an extracellular model plant pathogen, yet its potential to produce secreted effectors that manipulate the apoplast has been under investigated. here we identified 131 candidate small, secreted, non-annotated proteins from the ptodc3000 genome, most of which are common to pseudomonas species and potentially expressed during apoplastic colonization. we produced 43 of these proteins through a custom-made gateway-compatible expression system for ... | 2016 | 27603016 |
| the interaction between arbuscular mycorrhizal fungi and endophytic bacteria enhances plant growth of acacia gerrardii under salt stress. | microbes living symbiotically in plant tissues mutually cooperate with each other by providing nutrients for proliferation of the partner organism and have a beneficial effect on plant growth. however, few studies thus far have examined the interactive effect of endophytic bacteria and arbuscular mycorrhizal fungi (amf) in hostile conditions and their potential to improve plant stress tolerance. in this study, we investigated how the synergistic interactions of endophytic bacteria and amf affect ... | 2016 | 27486442 |
| gcsr, a tyrr-like enhancer-binding protein, regulates expression of the glycine cleavage system in pseudomonas aeruginosa pao1. | glycine serves as a major source of single carbon units for biochemical reactions within bacterial cells. utilization of glycine is tightly regulated and revolves around a key group of proteins known as the glycine cleavage system (gcs). our lab previously identified the transcriptional regulator gcsr (pa2449) as being required for catabolism of glycine in the opportunistic pathogen pseudomonas aeruginosa pao1. in an effort to clarify and have an overall better understanding of the role of gcsr ... | 2016 | 27303730 |
| protein domain architectures provide a fast, efficient and scalable alternative to sequence-based methods for comparative functional genomics. | a functional comparative genome analysis is essential to understand the mechanisms underlying bacterial evolution and adaptation. detection of functional orthologs using standard global sequence similarity methods faces several problems; the need for defining arbitrary acceptance thresholds for similarity and alignment length, lateral gene acquisition and the high computational cost for finding bi-directional best matches at a large scale. we investigated the use of protein domain architectures ... | 2016 | 27703668 |
| nad-independent l-lactate dehydrogenase required for l-lactate utilization in pseudomonas stutzeri a1501. | nad-independent l-lactate dehydrogenases (l-ildhs) play important roles in l-lactate utilization of different organisms. all of the previously reported l-ildhs were flavoproteins that catalyze the oxidation of l-lactate by the flavin mononucleotide (fmn)-dependent mechanism. based on comparative genomic analysis, a gene cluster with three genes (llda, lldb, and lldc) encoding a novel type of l-ildh was identified in pseudomonas stutzeri a1501. when the gene cluster was expressed in escherichia c ... | 2015 | 25917905 |
| microbial toluene removal in hypoxic model constructed wetlands occurs predominantly via the ring monooxygenation pathway. | in the present study, microbial toluene degradation in controlled constructed wetland model systems, planted fixed-bed reactors (pfrs), was queried with dna-based methods in combination with stable isotope fractionation analysis and characterization of toluene-degrading microbial isolates. two pfr replicates were operated with toluene as the sole external carbon and electron source for 2 years. the bulk redox conditions in these systems were hypoxic to anoxic. the autochthonous bacterial communi ... | 2015 | 26150458 |
| fleq dna binding consensus sequence revealed by studies of fleq-dependent regulation of biofilm gene expression in pseudomonas aeruginosa. | the transcription factor fleq from pseudomonas aeruginosa derepresses expression of genes involved in biofilm formation when intracellular levels of the second messenger cyclic diguanosine monophosphate (c-di-gmp) are high. fleq also activates transcription of flagellar genes, and the expression of these genes is highest at low intracellular c-di-gmp. fleq thus plays a central role in mediating the transition between planktonic and biofilm lifestyles of p. aeruginosa. previous work showed that f ... | 2015 | 26483521 |
| bioenergetics and the role of soluble cytochromes c for alkaline adaptation in gram-negative alkaliphilic pseudomonas. | very few studies have been conducted on alkaline adaptation of gram-negative alkaliphiles. the reversed difference of h(+) concentration across the membrane will make energy production considerably difficult for gram-negative as well as gram-positive bacteria. cells of the alkaliphilic gram-negative bacterium pseudomonas alcaliphila al15-21(t) grown at ph 10 under low-aeration intensity have a soluble cytochrome c content that is 3.6-fold higher than that of the cells grown at ph 7 under high-ae ... | 2015 | 25705691 |
| fleq dna binding consensus sequence revealed by studies of fleq-dependent regulation of biofilm gene expression in pseudomonas aeruginosa. | the transcription factor fleq from pseudomonas aeruginosa derepresses expression of genes involved in biofilm formation when intracellular levels of the second messenger cyclic diguanosine monophosphate (c-di-gmp) are high. fleq also activates transcription of flagellar genes, and the expression of these genes is highest at low intracellular c-di-gmp. fleq thus plays a central role in mediating the transition between planktonic and biofilm lifestyles of p. aeruginosa. previous work showed that f ... | 2015 | 26483521 |
| different ancestries of r tailocins in rhizospheric pseudomonas isolates. | bacterial genomes accommodate a variety of mobile genetic elements, including bacteriophage-related clusters that encode phage tail-like protein complexes playing a role in interactions with eukaryotic or prokaryotic cells. such tailocins are unable to replicate inside target cells due to the lack of a phage head with associated dna. a subset of tailocins mediate antagonistic activities with bacteriocin-like specificity. functional characterization of bactericidal tailocins of two pseudomonas pu ... | 2015 | 26412856 |
| diversity of pseudomonas genomes, including populus-associated isolates, as revealed by comparative genome analysis. | the pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. their diversity influences the phylogenetic diversity and heterogeneity of these communities. on the basis of average amino acid identity, comparative genome analysis of >1,000 pseudomonas genomes, including 21 pseudomonas strains isolated from the roots of native populus deltoides (eastern cottonwood) trees resu ... | 2015 | 26519390 |
| diversity of pseudomonas genomes, including populus-associated isolates, as revealed by comparative genome analysis. | the pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. their diversity influences the phylogenetic diversity and heterogeneity of these communities. on the basis of average amino acid identity, comparative genome analysis of >1,000 pseudomonas genomes, including 21 pseudomonas strains isolated from the roots of native populus deltoides (eastern cottonwood) trees resu ... | 2015 | 26519390 |
| recombinant lipases and phospholipases and their use as biocatalysts for industrial applications. | lipases and phospholipases are interfacial enzymes that hydrolyze hydrophobic ester linkages of triacylglycerols and phospholipids, respectively. in addition to their role as esterases, these enzymes catalyze a plethora of other reactions; indeed, lipases also catalyze esterification, transesterification and interesterification reactions, and phospholipases also show acyltransferase, transacylase and transphosphatidylation activities. thus, lipases and phospholipases represent versatile biocatal ... | 2015 | 26340621 |
| opportunistic pathogens and elements of the resistome that are common in bottled mineral water support the need for continuous surveillance. | several differences concerning bacterial species, opportunistic pathogens, elements of the resistome as well as variations concerning the cfu/ml counts were identified in some of the five most marketed bottled mineral water from araraquara city, são paulo, brazil. two out of five brands tested were confirmed as potential source of opportunistic pathogens, including mycobacterium gordonae, ralstonia picketti and burkholderia cepacia complex (bcc). a total of one hundred and six isolates were reco ... | 2015 | 25803794 |
| contamination of sulfonamide antibiotics and sulfamethazine-resistant bacteria in the downstream and estuarine areas of jiulong river in southeast china. | surface water samples from downstream and estuarine areas of jiulong river were collected in august 2011 and may 2012 for detecting sulfonamide antibiotic residues and isolating sulfamethazine-resistant bacteria. sulfamethazine was detected in all samples in may 2012 at an average concentration of 78.3 ng l(-1), which was the highest among the nine sulfonamide antibiotics determined. sulfamethazine-resistant bacteria (srb) were screened using antibiotic-containing agar plates. the srb average ab ... | 2015 | 25877900 |
| indole trimers with antibacterial activity against gram-positive organisms produced using combinatorial biocatalysis. | the i100v isoform of toluene-4-monooxygenase was used to catalyze the oxidative polymerization of anthranil and various indoles under mildly acidic conditions, favoring the production of trimers. compounds produced in sufficient yield were purified and tested for their ability to inhibit the growth of b. anthracis, e. faecalis, l. monocytogenes, s. aureus, and in some cases, f. tularensis. 15 of the compounds displayed promising antibacterial activity (mic < 5 µg/ml) against one or more of the s ... | 2015 | 26112315 |
| regulation of plasmid-encoded isoprene metabolism in rhodococcus, a representative of an important link in the global isoprene cycle. | emissions of biogenic volatile organic compounds (vocs) form an important part of the global carbon cycle, comprising a significant proportion of net ecosystem productivity. they impact atmospheric chemistry and contribute directly and indirectly to greenhouse gases. isoprene, emitted largely from plants, comprises one third of total vocs, yet in contrast to methane, which is released in similar quantities, we know little of its biodegradation. here, we report the genome of an isoprene degrading ... | 2015 | 25727256 |
| high voltage electrochemiluminescence (ecl) as a new method for detection of pah during screening for pah-degrading microbial consortia. | the search for new bacterial consortia capable of removing pah from the environment is associated with the need to employ novel, simple, and economically efficient detection methods. a fluorimetric method (fl) as well as high voltage electrochemiluminescence (ecl) on a modified surface of an aluminum electrode were used in order to determine the changes in the concentrations of pah in the studied aqueous solutions. the ecl signal (the spectrum and emission intensity for a given wavelength) was d ... | 2015 | 26213425 |
| structural and biochemical analysis of tyrosine phosphatase related to biofilm formation a (tpba) from the opportunistic pathogen pseudomonas aeruginosa pao1. | biofilms are important for cell communication and growth in most bacteria, and are responsible for a number of human clinical infections and diseases. tpba (pa3885) is a dual specific tyrosine phosphatase (dusp) that negatively regulates biofilm formation in the opportunistic pathogen pseudomonas aeruginosa pao1 by converting extracellular quorum sensing signals into internal gene cascade reactions that result in reduced biofilm formation. we have determined the three-dimensional crystal structu ... | 2015 | 25909591 |
| structure of aada from salmonella enterica: a monomeric aminoglycoside (3'')(9) adenyltransferase. | aminoglycoside resistance is commonly conferred by enzymatic modification of drugs by aminoglycoside-modifying enzymes such as aminoglycoside nucleotidyltransferases (ants). here, the first crystal structure of an ant(3'')(9) adenyltransferase, aada from salmonella enterica, is presented. aada catalyses the magnesium-dependent transfer of adenosine monophosphate from atp to the two chemically dissimilar drugs streptomycin and spectinomycin. the structure was solved using selenium sad phasing and ... | 2015 | 26527143 |
| presence of bacteria in failed anterior cruciate ligament reconstructions. | novel microbial detection technologies have revealed that chronic bacterial biofilms, which are recalcitrant to antibiotic treatment, are common in failed orthopedic procedures. | 2015 | 26339561 |
| efflux pump-deficient mutants as a platform to search for microbes that produce antibiotics. | pseudomonas putida dot-t1e-18 is a strain deficient in the major antibiotic efflux pump (ttgabc) that exhibits an overall increased susceptibility to a wide range of drugs when compared with the wild-type strain. we used this strain as a platform to search for microbes able to produce antibiotics that inhibit growth. a collection of 2400 isolates from soil, sediments and water was generated and a drop assay developed to identify, via growth inhibition halos, strains that prevent the growth of do ... | 2015 | 26059350 |
| microbial community in high arsenic shallow groundwater aquifers in hetao basin of inner mongolia, china. | a survey was carried out on the microbial community of 20 groundwater samples (4 low and 16 high arsenic groundwater) and 19 sediments from three boreholes (two high arsenic and one low arsenic boreholes) in a high arsenic groundwater system located in hetao basin, inner mongolia, using the 454 pyrosequencing approach. a total of 233,704 sequence reads were obtained and classified into 12-267 operational taxonomic units (otus). groundwater and sediment samples were divided into low and high arse ... | 2015 | 25970606 |
| diversity and role of plasmids in adaptation of bacteria inhabiting the lubin copper mine in poland, an environment rich in heavy metals. | the lubin underground mine, is one of three mining divisions in the lubin-glogow copper district in lower silesia province (poland). it is the source of polymetallic ore that is rich in copper, silver and several heavy metals. black shale is also significantly enriched in fossil organic matter in the form of long-chain hydrocarbons, polycyclic aromatic hydrocarbons, organic acids, esters, thiophenes and metalloporphyrins. biological analyses have revealed that this environment is inhabited by ex ... | 2015 | 26074880 |
| malpighian tubules are important determinants of pseudomonas transstadial transmission and longtime persistence in anopheles stephensi. | pseudomonas is a genus of bacteria commonly found in investigations of gut microbes in malaria mosquitoes. among those mosquitoes is the dominating malaria vector in asia, anopheles stephensi, where pseudomonas is a prevailing bacterium and natural inhabitant of its breeding places. in order to explore the reason for finding pseudomonas so frequently, an investigation of its localization and transstadial properties was undertaken. | 2015 | 25604581 |
| an antimicrobial metabolite from bacillus sp.: significant activity against pathogenic bacteria including multidrug-resistant clinical strains. | in this study, the cell free modified tryptone soya broth (ph 7.4 ± 0.2) of bacillus subtilis urid 12.1 showed significant antimicrobial activity against multidrug-resistant strains of staphylococcus aureus, s. epidermidis, streptococcus pyogenes and enterococcus faecalis. the partially purified antimicrobial molecule was found to be resistant to extremes of ph and temperatures and also to higher concentrations of trypsin and proteinase k. the antimicrobial molecule was purified by a three-step ... | 2015 | 26696963 |
| the yhs-domain of an adenylyl cyclase from mycobacterium phlei is a probable copper-sensor module. | yhs-domains are small protein modules which have been proposed to bind transition-metal ions like the related trash-domains. they are found in a variety of enzymes including copper-transporting atpases and adenylyl cyclases. here we investigate a class iiic adenylyl cyclase from mycobacterium phlei which contains a c-terminal yhs-domain linked to the catalytic domain by a peptide of 8 amino acids. we expressed the isolated catalytic domain and the full-length enzyme in e. coli. the catalytic dom ... | 2015 | 26512893 |
| community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps. | soluble arsenic (as) in acidic feed solution may inhibit the copper (cu) bioleaching process within mine heaps. to clarify the effect of soluble arsenic on the live biomass and bioxidative activity in heaps, toxicological assays were performed using a synthetic feed solution given by a mine company. the microorganisms had previously been isolated from two heap samples at up to 66 m depth, and cultured using specific media for chemolithotrophic acidophiles (ph 1-2) and moderate thermophiles (48°c ... | 2015 | 26283066 |
| genomic analysis reveals the biotechnological and industrial potential of levan producing halophilic extremophile, halomonas smyrnensis aad6t. | halomonas smyrnensis aad6t is a gram negative, aerobic, and moderately halophilic bacterium, and is known to produce high levels of levan with many potential uses in foods, feeds, cosmetics, pharmaceutical and chemical industries due to its outstanding properties. here, the whole-genome analysis was performed to gain more insight about the biological mechanisms, and the whole-genome organization of the bacterium. industrially crucial genes, including the levansucrase, were detected and the genom ... | 2015 | 26251777 |
| characterization of pkp-m1144, a novel cole1-like plasmid encoding imp-8, ges-5, and bel-1 β-lactamases, from a klebsiella pneumoniae sequence type 252 isolate. | imp-8 metallo-β-lactamase was identified in klebsiella pneumoniae sequence type 252 (st252), isolated in a portuguese hospital in 2009. blaimp-8 was the first gene cassette of a novel class 3 integron, in1144, also carrying the blages-5, blabel-1, and aaca4 cassettes. in1144 was located on a cole1-like plasmid, pkp-m1144 (12,029 bp), with a replication region of limited nucleotide similarity to those of other rna-priming plasmids, such as pjhcmw1. in1144 and pkp-m1144 represent an interesting ca ... | 2015 | 26033721 |
| diagnostic value of bronchoalveolar lavage in leukemic and bone marrow transplant patients: the impact of antimicrobial therapy. | there is significant morbidity and mortality from pneumonia in leukemic and bone marrow transplant patients. we sought to explore the diagnostic yield of bronchoalveolar lavage (bal) in these patients with new pulmonary infiltrates. a retrospective chart review of approximately 200 non- human immunodeficiency virus (hiv) leukemic and hematopoietic stem cell transplantation (hsct) patients who underwent bronchoscopy at a single academic cancer center was performed. antimicrobial use for less than ... | 2015 | 25574361 |
| approaches to studying and manipulating the enteric microbiome to improve autism symptoms. | there is a growing body of scientific evidence that the health of the microbiome (the trillions of microbes that inhabit the human host) plays an important role in maintaining the health of the host and that disruptions in the microbiome may play a role in certain disease processes. an increasing number of research studies have provided evidence that the composition of the gut (enteric) microbiome (gm) in at least a subset of individuals with autism spectrum disorder (asd) deviates from what is ... | 2015 | 25956237 |
| recovery of critical metals using biometallurgy. | the increased development of green low-carbon energy technologies that require platinum group metals (pgms) and rare earth elements (rees), together with the geopolitical challenges to sourcing these metals, has spawned major governmental and industrial efforts to rectify current supply insecurities. as a result of the increasing critical importance of pgms and rees, environmentally sustainable approaches to recover these metals from primary ores and secondary streams are needed. in this review, ... | 2015 | 25912797 |
| pan-genome dynamics of pseudomonas gene complements enriched across hexachlorocyclohexane dumpsite. | phylogenetic heterogeneity across pseudomonas genus is complemented by its diverse genome architecture enriched by accessory genetic elements (plasmids, transposons, and integrons) conferring resistance across this genus. here, we sequenced a stress tolerant genotype i.e. pseudomonas sp. strain rl isolated from a hexachlorocyclohexane (hch) contaminated pond (45 mg of total hch g(-1) sediment) and further compared its gene repertoire with 17 reference ecotypes belonging to p. stutzeri, p. mendoc ... | 2015 | 25898829 |
| nitrogen removal from micro-polluted reservoir water by indigenous aerobic denitrifiers. | treatment of micro-polluted source water is receiving increasing attention because of environmental awareness on a global level. we isolated and identified aerobic denitrifying bacteria zoogloea sp. n299, acinetobacter sp. g107, and acinetobacter sp. 81y and used these to remediate samples of their native source water. we first domesticated the isolated strains in the source water, and the 48-h nitrate removal rates of strains n299, g107, and 81y reached 33.69%, 28.28%, and 22.86%, respectively, ... | 2015 | 25867475 |
| draft genome sequence of pseudomonas toyotomiensis kf710, a polychlorinated biphenyl-degrading bacterium isolated from biphenyl-contaminated soil. | pseudomonas toyotomiensis kf710 utilizes biphenyl and degrades polychlorinated biphenyls (pcbs). here, we report the genome sequence of the kf710 strain, consisting of 5,596,721 bp with 5,155 coding sequences. the biphenyl catabolic genes were almost identical to those of pseudomonas pseudoalcaligenes kf707, one of the most well-characterized biphenyl-utilizing strains. | 2015 | 25838482 |
| optimization of medium composition for 3-hydroxycarboxylic acid production by pseudomonas mendocina-biodegraded polyhydroxybutyrate. | we optimized the culture medium for 3-hydroxycarboxylic acid production by pseudomonas mendocina ds-04-t-biodegraded polyhydroxybutyrate (phb) using the plackett-burman design, steepest ascent method, and box-behnken design. the optimized concentrations of the constituents of the culture medium were as follows: phb (7.57 g/l), nh4 cl (5.0 g/l), kh2 po4 (2.64 g/l), na2 hpo4 ·12h2 o (12 g/l), mgso4 ·7h2 o (0.5 g/l), and cacl2 ·2h2 o (5 mg/l). the yield of 3-hydroxycarboxylic acid obtained using th ... | 2015 | 24919602 |
| photoassisted biodegradation of irradiated organics in simulated nuclear wastewater. | the extent of dehalogenation and degradation of toxic aromatic compounds in a nuclear wastewater was evaluated using a two-stage system consisting of a photolytic reactor followed by a biological reactor. experiments were performed by varying the initial 4-chlorophenol (4-cp) concentration from 50 to 1000 mg/l. the uv pretreatment stage improved the overall efficiency of biodegradation of the recalcitrant compound by facilitating degradability in the biological stage. removal efficiencies greate ... | 2015 | 26460459 |
| an upp-based markerless gene replacement method for genome reduction and metabolic pathway engineering in pseudomonas mendocina nk-01 and pseudomonas putida kt2440. | a markerless gene replacement method was adapted by combining a suicide plasmid, pex18tc, with a counterselectable marker, the upp gene encoding uracil phosphoribosyltransferase (uprtase), for the medium-chain length polyhydroxyalkanoates (pha(mcl))-producing strain pseudomonas mendocina nk-01. an nk-01 5-fluorouracil (5-fu) resistant background strain was first constructed by deleting the chromosomal upp gene. the suicide plasmid pex18tc, carrying a functional allele of the upp gene of p. mendo ... | 2015 | 25828098 |
| characterization of the ability to form biofilms by plant-associated pseudomonas species. | successful colonization is the initial step for plant-bacteria interactions; therefore, the development of strategies to improve adherence to plant surfaces is critically important for environmental bacteria. biofilm formation is thought to be one such strategy for bacteria to establish stable colonization on inert and living surfaces. although biofilms play potential roles in enabling persistent bacterial colonization, little attention has been paid to biofilms formed by plant-associated bacter ... | 2015 | 25487118 |
| bacterial community analysis in chlorpyrifos enrichment cultures via dgge and use of bacterial consortium for cp biodegradation. | the organophosphate pesticide chlorpyrifos (cp) has been used extensively since the 1960s for insect control. however, its toxic effects on mammals and persistence in environment necessitate its removal from contaminated sites, biodegradation studies of cp-degrading microbes are therefore of immense importance. samples from a pakistani agricultural soil with an extensive history of cp application were used to prepare enrichment cultures using cp as sole carbon source for bacterial community anal ... | 2014 | 25008559 |
| emulating structural stability of pseudomonas mendocina lipase: in silico mutagenesis and molecular dynamics studies. | the need of alkaline detergent-stable lipases has been growing rapidly as they are highly attractive for the production of detergents, biodiesel, pharmaceuticals agents, and various other applications. lipase from pseudomonas mendocina (pml) is one such candidate with triglyceride activity and non-homologous with other reported pseudomonas lipases. the present work provides insights on the role of amino acids toward structural stability of pml. pml was subjected to mutagenesis through in silico ... | 2014 | 25367042 |
| nitric oxide removal by wastewater bacteria in a biotrickling filter. | nitric oxide (no) is one of the most important air pollutants in atmosphere mainly emitted from combustion source. a biotrickling filter was designed and operated to remove no from an air stream using bacteria extracted from the sewage sludge of a municipal sewage treatment plant. to obtain the best operation conditions for the biotrickling filter, orthogonal experiments (l9(3(4))) were designed. inlet oxygen concentration was found to be the most significant factor of the biotrickling filter an ... | 2014 | 25079268 |
| calcium-mediated modulation of pseudomonas mendocina nr802 biofilm influences the phenanthrene degradation. | a potential biofilm forming and phenanthrene utilizing marine bacterium pseudomonas mendocina nr802 was isolated from rushukulya, odisha, east coast of india. the effect of ca(2+) and mg(2+) on biofilm growth and phenanthrene degradation was evaluated. among the various tested concentrations, 20 mm of ca(2+) and mg(2+) showed a significant enhancement in biofilm production by the bacterium. the sem-edax study showed that the elemental composition of the biofilm varied significantly when grown in ... | 2014 | 24216621 |
| enhanced anaerobic biodegradation of ocdd-contaminated soils by pseudomonas mendocina nsysu: microcosm, pilot-scale, and gene studies. | in this study, microcosm and pilot-scale experiments were performed to investigate the capability and effectiveness of pseudomonas mendocina nsysu (p. mendocina nsysu) on the bioremediation of octachlorodibenzo-p-dioxin (ocdd)-contaminated soils. the objectives were to evaluate the (1) characteristics of p. mendocina nsysu, (2) feasibility of enhancing ocdd biodegradation with the addition of p. mendocina nsysu and lecithin, and (3) variation in microbial diversity and genes responsible for the ... | 2014 | 24997259 |
| aerobic microbial fe acquisition from ferrihydrite nanoparticles: effects of crystalline order, siderophores, and alginate. | this research compared the bioavailability of fe associated with two forms of the hydrous fe oxyhydroxide nanomineral ferrihydrite (fh)--the smaller (1-3 nm), less ordered 2-line (2l) phase and the slightly larger, (2-6 nm) more ordered 6-line (6l) phase--to the common aerobic soil bacterium pseudomonas mendocina ymp. p. mendocina can acquire fe from minerals using high-affinity fe(iii) binding ligands known as siderophores and a cell-associated metalloreductase that requires direct cell-mineral ... | 2014 | 24978298 |
| saturation mutagenesis of bradyrhizobium sp. btai1 toluene 4-monooxygenase at alpha-subunit residues proline 101, proline 103, and histidine 214 for regiospecific oxidation of aromatics. | a novel toluene monooxygenase (tmo) six-gene cluster from bradyrhizobium sp. btai1 having an overall 35, 36, and 38 % protein similarity with toluene o-xylene monooxygenase (tomo) of pseudomonas sp. ox1, toluene 4-monooxygenase (t4mo) of pseudomonas mendocina kr1, and toluene-para-monooxygenase (tpmo) of ralstonia pickettii pko1, respectively, was cloned and expressed in escherichia coli tg1, and its potential activity was investigated for aromatic hydroxylation and trichloroethylene (tce) degra ... | 2014 | 25016343 |
| microbial enzymes: tools for biotechnological processes. | microbial enzymes are of great importance in the development of industrial bioprocesses. current applications are focused on many different markets including pulp and paper, leather, detergents and textiles, pharmaceuticals, chemical, food and beverages, biofuels, animal feed and personal care, among others. today there is a need for new, improved or/and more versatile enzymes in order to develop more novel, sustainable and economically competitive production processes. microbial diversity and m ... | 2014 | 24970208 |
| sibling rivalry: related bacterial small rnas and their redundant and non-redundant roles. | small rna molecules (srnas) are now recognized as key regulators controlling bacterial gene expression, as srnas provide a quick and efficient means of positively or negatively altering the expression of specific genes. to date, numerous srnas have been identified and characterized in a myriad of bacterial species, but more recently, a theme in bacterial srnas has emerged: the presence of more than one highly related srnas produced by a given bacterium, here termed sibling srnas. sibling srnas a ... | 2014 | 25389522 |
| a system to automatically classify and name any individual genome-sequenced organism independently of current biological classification and nomenclature. | a broadly accepted and stable biological classification system is a prerequisite for biological sciences. it provides the means to describe and communicate about life without ambiguity. current biological classification and nomenclature use the species as the basic unit and require lengthy and laborious species descriptions before newly discovered organisms can be assigned to a species and be named. the current system is thus inadequate to classify and name the immense genetic diversity within s ... | 2014 | 24586551 |
| is there still room for novel viral pathogens in pediatric respiratory tract infections? | viruses are the most frequent cause of respiratory disease in children. however, despite the advanced diagnostic methods currently in use, in 20 to 50% of respiratory samples a specific pathogen cannot be detected. in this work, we used a metagenomic approach and deep sequencing to examine respiratory samples from children with lower and upper respiratory tract infections that had been previously found negative for 6 bacteria and 15 respiratory viruses by pcr. nasal washings from 25 children (ou ... | 2014 | 25412469 |
| isolation, identification and characterization of burkholderia pseudomallei from soil of coastal region of india. | melioidosis is an emerging infectious disease caused by a free living soil dwelling gram-negative bacterium burkholderia pseudomallei. the disease is endemic to most parts of southeast asia and northern australia and the organism has been isolated from moist soil and water. in india clinical cases are recently reported from the states of tamilnadu, kerala, karnataka, maharashtra, orissa, assam, west bengal, pondicherry and tripura. this study is aimed to confirm the prevalence of this important ... | 2014 | 25187882 |
| organic solvent tolerant lipases and applications. | lipases are a group of enzymes naturally endowed with the property of performing reactions in aqueous as well as organic solvents. the esterification reactions using lipase(s) could be performed in water-restricted organic media as organic solvent(s) not only improve(s) the solubility of substrate and reactant in reaction mixture but also permit(s) the reaction in the reverse direction, and often it is easy to recover the product in organic phase in two-phase equilibrium systems. the use of orga ... | 2014 | 24672342 |
| biogps descriptors for rational engineering of enzyme promiscuity and structure based bioinformatic analysis. | a new bioinformatic methodology was developed founded on the unsupervised pattern cognition analysis of grid-based biogps descriptors (global positioning system in biological space). the procedure relies entirely on three-dimensional structure analysis of enzymes and does not stem from sequence or structure alignment. the biogps descriptors account for chemical, geometrical and physical-chemical features of enzymes and are able to describe comprehensively the active site of enzymes in terms of " ... | 2014 | 25353170 |
| fast proton-coupled electron transfer observed for a high-fidelity structural and functional [2fe-2s] rieske model. | rieske cofactors have a [2fe-2s] cluster with unique {his2cys2} ligation and distinct fe subsites. the histidine ligands are functionally relevant, since they allow for coupling of electron and proton transfer (pcet) during quinol oxidation in respiratory and photosynthetic et chains. here we present the highest fidelity synthetic analogue for the rieske [2fe-2s] cluster reported so far. this synthetic analogue 5(x-) emulates the heteroleptic {his2cys2} ligation of the [2fe-2s] core, and it also ... | 2014 | 24506804 |
| a pseudomonas putida strain genetically engineered for 1,2,3-trichloropropane bioremediation. | 1,2,3-trichloropropane (tcp) is a toxic compound that is recalcitrant to biodegradation in the environment. attempts to isolate tcp-degrading organisms using enrichment cultivation have failed. a potential biodegradation pathway starts with hydrolytic dehalogenation to 2,3-dichloro-1-propanol (dcp), followed by oxidative metabolism. to obtain a practically applicable tcp-degrading organism, we introduced an engineered haloalkane dehalogenase with improved tcp degradation activity into the dcp-de ... | 2014 | 24973068 |
| diagnosis of prosthetic joint infection by use of pcr-electrospray ionization mass spectrometry. | we compared pcr-electrospray ionization mass spectrometry (pcr-esi/ms) to culture using sonicate fluid from 431 subjects with explanted knee (n = 270) or hip (n = 161) prostheses. of these, 152 and 279 subjects had prosthetic joint infection (pji) and aseptic failure, respectively. the sensitivities for detecting pji were 77.6% for pcr-esi/ms and 69.7% for culture (p = 0.0105). the specificities were 93.5 and 99.3%, respectively (p = 0.0002). | 2014 | 24478503 |
| draft genome sequence of petroleum oil-degrading marine bacterium pseudomonas taeanensis strain ms-3, isolated from a crude oil-contaminated seashore. | pseudomonas taeanensis ms-3(t), isolated from a crude oil-contaminated seashore in south korea, is capable of degrading petroleum oils, such as gasoline, diesel, and kerosene. here, we report the draft genome sequence of this strain, which consists of 5,477,045 bp, with a g+c content of 60.72%. | 2014 | 24407626 |
| escherichia coli with bla(imp-8) in singapore. | 2014 | 24145544 | |
| (s)-3-hydroxyacyl-coa dehydrogenase/enoyl-coa hydratase (fadb') from fatty acid degradation operon of ralstonia eutropha h16. | in this study (s)-3-hydroxyacyl-coa dehydrogenase/enoyl-coa hydratase (h16_a0461/fadb', gene id: 4247876) from one of two active fatty acid degradation operons of ralstonia eutropha h16 has been heterologously expressed in escherichia coli, purified as protein possessing a his-tag and initially characterized. fadb' is an enzyme with two catalytic domains exhibiting a single monomeric structure and possessing a molecular weight of 86 kda. the c-terminal part of the enzyme harbors enoyl-coa hydrat ... | 2014 | 25401070 |
| impact of next generation sequencing techniques in food microbiology. | understanding the maxam-gilbert and sanger sequencing as the first generation, in recent years there has been an explosion of newly-developed sequencing strategies, which are usually referred to as next generation sequencing (ngs) techniques. ngs techniques have high-throughputs and produce thousands or even millions of sequences at the same time. these sequences allow for the accurate identification of microbial taxa, including uncultivable organisms and those present in small numbers. in speci ... | 2014 | 25132799 |
| ecobiotechnological strategy to enhance efficiency of bioconversion of wastes into hydrogen and methane. | vegetable wastes (vw) and food wastes (fw) are generated in large quantities by municipal markets, restaurants and hotels. waste slurries (250 ml) in 300 ml bod bottles, containing 3, 5 and 7 % total solids (ts) were hydrolyzed with bacterial mixtures composed of: bacillus, acinetobacter, exiguobacterium, pseudomonas, stenotrophomonas and sphingobacterium species. each of these bacteria had high activities for the hydrolytic enzymes: amylase, protease and lipase. hydrolysate of biowaste slurries ... | 2014 | 24891732 |
| an airborne actinobacteria nocardiopsis alba isolated from bioaerosol of a mushroom compost facility. | actinobacteria are widely distributed in many environments and represent the most important trigger to the occupant respiratory health. health complaints, including hypersensitivity pneumonitis of the workers, were recorded in a mushroom compost facility (mcf). the studies on the airborne bacteria were carried out to find a possible microbiological source of these symptoms. culture analysis of compost bioaerosols collected in different location of the mcf was performed. an assessment of the indo ... | 2014 | 25382928 |
| abattoirs as non-hospital source of extended spectrum beta lactamase producers: confirmed by the double disc synergy test and characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry. | in this study, the presence of extended spectrum beta lactamase (esbl) producing organisms in abattoirs, a non-hospital community was investigated. the presence of esbl-producing phenotypes was confirmed by the double disc synergy test (ddst). out of the 99 isolates screened for esbl, 28 (28.3%) were confirmed positive. the positive isolates were characterised by using matrix-assisted laser desorption/ionization time of flight mass spectrometry. 50% of the isolates were pseudomonas spp., the res ... | 2014 | 24728403 |
| from structure to catalysis: recent developments in the biotechnological applications of lipases. | microbial lipases are highly appreciated as biocatalysts due to their peculiar characteristics such as the ability to utilize a wide range of substrates, high activity and stability in organic solvents, and regio- and/or enantioselectivity. these enzymes are currently being applied in a variety of biotechnological processes, including detergent preparation, cosmetics and paper production, food processing, biodiesel and biopolymer synthesis, and the biocatalytic resolution of pharmaceutical deriv ... | 2014 | 24783219 |
| emergence of citrobacter freundii carrying imp-8 metallo-β-lactamase in germany. | metallo-β-lactamases (mbls) in enterobacteriaceae are an increasing problem worldwide. this report describes the isolation of citrobacter freundii carrying imp-8 mbl from three patients during the period from march 2012 until march 2013 in germany. the bla imp-8 enzyme is predominantly found in asia, where imp-8 has spread to various enterobacterial species causing serious infections. to our best knowledge, this is the first report of bla imp-8 habouring enterobacteriaceae in europe. | 2014 | 25356340 |
| t cell epitope mimicry between sjögren's syndrome antigen a (ssa)/ro60 and oral, gut, skin and vaginal bacteria. | this study was undertaken to test the hypothesis that sjogren's syndrome antigen a (ssa)/ro60-reactive t cells are activated by peptides originating from oral and gut bacteria. t cell hybridomas generated from hla-dr3 transgenic mice recognized 3 regions on ro60, with core epitopes mapped to amino acids 228-238, 246-256 and 371-381. blast analysis identified several mimicry peptides, originating from human oral, intestinal, skin and vaginal bacteria, as well as environmental bacteria. amongst th ... | 2014 | 24576620 |
| microbial ecology of anaerobic digesters: the key players of anaerobiosis. | anaerobic digestion is the method of wastes treatment aimed at a reduction of their hazardous effects on the biosphere. the mutualistic behavior of various anaerobic microorganisms results in the decomposition of complex organic substances into simple, chemically stabilized compounds, mainly methane and co2. the conversions of complex organic compounds to ch4 and co2 are possible due to the cooperation of four different groups of microorganisms, that is, fermentative, syntrophic, acetogenic, and ... | 2014 | 24701142 |
| adsorption studies of the gram-negative bacteria onto nanostructured silicon carbide. | in this study, we demonstrated a significant adsorption of pseudomonas putida bacteria onto aggregates of nanofibers (nfsic) and nanorods (nrsic) of silicon carbide (sic) in aqueous suspensions. langmuir and freundlich isotherms were used to quantify adsorption affinities. it was found that adsorption of the bacteria strongly depended on the structure of the silicon carbide and the ph of the aqueous solution, which affected the isoelectric point of both the silicon carbide and the bacterial cell ... | 2014 | 25410802 |