Publications

prevalence of netf-positive clostridium perfringens in foals in southwestern ontario.	netf-producing clostridium perfringens have recently been identified as a cause of necrotizing enteritis in neonatal foals, but little is known about its prevalence in clinically normal foals. foals (n = 88) ranging in age from < 1 wk to 2 to 4 mo (median age 2 to 4 wk) on 8 horse-breeding farms in ontario were examined on 1 or 2 occasions for the presence of c. perfringens. of the foals that tested positive, 5 isolates (n = 675) were examined for the netf and enterotoxin (cpe) genes. colonizati ...	2016	27408339
antimicrobial susceptibility of clinical isolates of anaerobic bacteria in ontario, 2010-2011.	the local epidemiology of antimicrobial susceptibility patterns in anaerobic bacteria is important in guiding the empiric treatment of infections. however, susceptibility data are very limited on anaerobic organisms, particularly among non-bacteroides organisms. to determine susceptibility profiles of clinically-significant anaerobic bacteria in ontario canada, anaerobic isolates from sterile sites submitted to public health ontario laboratory (phol) for identification and susceptibility testing ...	2014	24923267
a retrospective study on the etiological diagnoses of diarrhea in neonatal piglets in ontario, canada, between 2001 and 2010.	laboratory surveillance data from the animal health laboratory, university of guelph, on the etiological diagnoses of neonatal diarrhea in piglets were analyzed to determine the relative importance and trends of different enteric pathogens in ontario. a total of 237 cases, including live and dead 1- to 7-day-old piglets, were submitted for diagnosis of gastrointestinal illness between 2001 and 2010. the combined frequencies for cases of gastrointestinal illness involving escherichia coli, clostr ...	2013	24124267
the epidemiology of clostridium perfringens type a on ontario swine farms, with special reference to cpb2-positive isolates.	there is poor understanding of most aspects of clostridium perfringens type a as a possible cause of neonatal diarrhea in piglets, and the prevalence and types of c. perfringens present on ontario swine farms is unknown. to study the prevalence of fecal c. perfringens and selected toxin genes, 48 ontario swine farms were visited between august 2010 and may 2011, and 354 fecal samples were collected from suckling pigs, lactating sows, weanling pigs, grower-finisher pigs, and gestating sows, as we ...	2012	22947389
pork implicated in a shiga toxin-producing escherichia coli o157:h7 outbreak in ontario, canada.	to describe an outbreak of shiga toxin-producing escherichia coli (stec) o157:h7 infection following a four-day family gathering in ontario. this is the first published account of a stec o157 outbreak in canada linked to consumption of pork.	2012	23617981
antimicrobial susceptibility of clostridium perfringens isolates of bovine, chicken, porcine, and turkey origin from ontario.	antimicrobial susceptibilities and toxin types were determined for 275 clostridium perfringens isolates collected in ontario in the spring of 2005. minimal inhibitory concentrations (mics) of c. perfringens isolates for 12 antimicrobials used in therapy, prophylaxis, and/or growth promotion of cattle (n = 40), swine (n = 75), turkeys (n = 50), and chickens (n = 100) were determined using the microbroth dilution method. statistical analyses and mic distributions showed reduced susceptibility to b ...	2011	21731178
clostridium perfringens in retail chicken.	clostridium perfringens isolates were recovered by enrichment from retail grocery chicken samples (n = 88) in ontario, canada, with one sample per site. the gene associated with necrotic enteritis in chickens, netb, was found in 21% of the isolates. the tpel gene was found in 2% and the cpb2 gene in 68% (95% "atypical" genes) of isolates. this study suggests that netb-positive c. perfringens can reach people through retail chicken.	2010	19961943
seasonal relationships among indicator bacteria, pathogenic bacteria, cryptosporidium oocysts, giardia cysts, and hydrological indices for surface waters within an agricultural landscape.	the south nation river basin in eastern ontario, canada is characterized by mixed agriculture. over 1600 water samples were collected on a bi-weekly basis from up to 24 discrete sampling sites on river tributaries of varying stream order within the river basin between 2004 and 2006. water samples were analyzed for: densities of indicator bacteria (escherichia coli, clostridium perfringens, enterococci, total and fecal coliforms), the presence of pathogenic bacteria (listeria monocytogenes, e. co ...	2009	19339033
multilocus sequence typing analysis of clostridium perfringens isolates from necrotic enteritis outbreaks in broiler chicken populations.	clostridium perfringens is an important pathogen of animals and humans and is the causative agent of necrotic enteritis (ne) in poultry. this study focuses on the typing of intestinal c. perfringens isolates (n = 61) from outbreaks of ne collected from several areas of southern ontario, using a recently developed multilocus sequence typing (mlst) technique. for comparison, c. perfringens isolates from healthy birds were also obtained and typed. an additional locus, the pfos locus, was included i ...	2008	18945840
pathogen and indicator variability in a heavily impacted watershed.	water samples were collected from 36 locations within the grand river watershed, in southwestern ontario, canada from july 2002 to december 2003 and were analyzed for total coliforms, fecal coliforms, escherichia coli, escherichia coli o157:h7, and thermophilic campylobacter spp. a subset of samples was also analyzed for cryptosporidium spp., giardia spp., culturable human enteric viruses, and clostridium perfringens. storm and snowmelt events were sampled at two locations including a drinking w ...	2007	17674573
a prospective study of the roles of clostridium difficile and enterotoxigenic clostridium perfringens in equine diarrhoea.	faecal samples from adult horses and from foals with diarrhoea or with normal faeces were evaluated for the presence of clostridium difficile, c. difficile toxins, c. perfringens enterotoxin (cpe) and c. perfringens spore counts. clostridium difficile was isolated from 7/55 horses (12.7%) and 11/31 foals (35.5%) with colitis, but from 1/255 normal adults (0.4%) and 0/47 normal foals (p<0.001). clostridium difficile toxins a and/or b were detected in 12/55 diarrhoeic adults (21.8%) and 5/30 diarr ...	2001	11469775
multiple typing techniques applied to a clostridium perfringens food poisoning outbreak.	twenty-one stool specimens obtained from persons implicated in two food poisoning outbreaks at the same institution in smith falls, ontario, were examined for clostridium perfringens. ninety-two colonies of cl. perfringens (3-5 per stool specimen) were typed with antisera, bacteriocins and by plasmid analysis. they were also tested for the in vitro production of bacteriocin and enterotoxin. sixteen of the 21 stool specimens were tested directly for enterotoxin. this was detected in 13, five of w ...	1992	1517172
two outbreaks of clostridium perfringens food poisoning-ontario.		1990	2317881
laboratory confirmation of an outbreak of clostridium perfringens food poisoning.	a foodborne outbreak in a group of eleven individuals sharing a common meal, eight of whom reported symptoms and an incubation period compatible with clostridium perfringens food poisoning, was confirmed by the following laboratory criteria: (a) the c. perfringens count in the suspect food, chicken pie, was at least 10(5) per gram; (b) the median fecal c. perfringens spore count in convalescing patients was greater than 10(6) per gram: (c) an indentical serotype (ps63) was isolated from both the ...	1977	188789
acute renal failure in obstetrics.		1974	4358923
necrotic enteritis in broiler chickens. i. a review of the literature and the prevalence of the disease in ontario.	the available literature on necrotic enteritis in broiler chickens is reviewed. the records of three poultry diagnostic laboratories in ontario were examined for the years 1969, 1970 and 1971. during this period 855 (7.7%) of 11,076 consignments of broiler chickens examined were diagnosed as necrotic enteritis. the condition was most common in broilers at three weeks of age, with 66% of all cases occurring in chickens between two and four and one half weeks. the disease occurred throughout the y ...	1973	4355471