Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
the n-terminal replacement of an olfactory receptor for the development of a yeast-based biomimetic odor sensor.for the development of a biomimetic odor-sensing system, we investigated the effects of replacing the n-terminus of an olfactory receptor (or) on its functional expression in the budding yeast, saccharomyces cerevisiae. using the mouse olfactory receptor or226 (mor226), three types of chimeric ors were constructed by replacing n-terminal regions of mor226 with the corresponding regions of the rat i7 receptor, which is known to be functionally expressed in yeast. the replacement of the n-terminal ...201221915853
safety evaluation of a proprietary food-grade, dried fermentate preparation of saccharomyces cerevisiae.a safety evaluation was performed for epicor, a product produced by a proprietary fermentation process using saccharomyces cerevisiae. studies included the following assays: bacterial reverse mutation, mouse lymphoma cell mutagenicity, mitogenicity assay in human peripheral lymphocytes, and a cytochrome p450 ([cyp] cyp1a2 and cyp3a4) induction assessment as well as 14-day acute, 90-day subchronic, and 1-year chronic oral toxicity studies in rats. no evidence of genotoxicity or mitogenicity was s ...201222222580
comparison of two expression systems using cos7 cells and yeast cells for expression of heart/muscle-type carnitine palmitoyltransferase 1.carnitine palmitoyltransferase 1 (cpt1), catalyzing the transfer of the acyl group from acyl-coa to carnitine to form acylcarnitine, is located at the outer mitochondrial membrane. because it is easily inactivated by solubilization, expression systems using living cells are essential for its functional characterization. cos7 cells or yeast cells are often utilized for this purpose; however, the advantages/disadvantages of the use of these cells or the question as to how the cpt1 enzyme expressed ...201222266133
distinct regulation of p53-mediated apoptosis by protein kinase ca, d, e and ?: evidence in yeast for transcription-dependent and -independent p53 apoptotic mechanisms.the role of individual protein kinase c (pkc) isoforms in the regulation of p53-mediated apoptosis is still uncertain. using yeast cells co-expressing the human wild-type p53 and a single mammalian pkca, d, e or ?, we showed a differential regulation of p53-mediated apoptosis by these pkc isoforms. whereas pkca and ? had no effect on p53 activity, pkcd and e stimulated a p53-mediated mitochondria-dependent apoptosis. moreover, using pifithrin-a and -µ, selective inhibitors of p53 transcriptional ...201121338602
development of chromanes as novel inhibitors of the uncoupling proteins.the uncoupling proteins (ucps) are mitochondrial carriers that modulate the energetic efficiency and, as a result, can lower superoxide levels. here, we describe the discovery of a small-molecule inhibitor of the ucps. screening of potential ucp1 regulators led to the identification of chromane derivatives that inhibit its proton conductance. members of the ucp family can act as a defense against oxidative stress and, thus, ucp2 plays a protective role in tumor cells. high ucp2 levels have been ...201121338923
xenotransplantation of mitochondrial electron transfer enzyme, ndi1, in myocardial reperfusion injury.a significant consequence of ischemia/reperfusion (i/r) is mitochondrial respiratory dysfunction, leading to energetic deficits and cellular toxicity from reactive oxygen species (ros). mammalian complex i, a nadh-quinone oxidoreductase enzyme, is a multiple subunit enzyme that oxidizes nadh and pumps protons across the inner membrane. damage to complex i leads to superoxide production which further damages complex i as well as other proteins, lipids and mtdna. the yeast, s. cerevisiae, expresse ...201121339825
pharmacokinetic-pharmacodynamic modeling of the inhibitory effects of naproxen on the time-courses of inflammatory pain, fever, and the ex vivo synthesis of txb2 and pge2 in rats.to quantify and compare the time-course and potency of the analgesic and antipyretic effects of naproxen in conjunction with the inhibition of pge(2) and txb(2).201121347567
interaction of selenite and tellurite with thiol-dependent redox enzymes: kinetics and mitochondrial implications.the interactions of selenite and tellurite with cytosolic and mitochondrial thioredoxin reductases (trxr1 and trxr2) and glutathione reductases (gr) from yeast and mammalian sources were explored. both trxr1 and trxr2 act as selenite and tellurite reductases. kinetic treatment shows that selenite has a greater affinity than tellurite with both trxr1 and trxr2. considering both k(cat) and k(m), selenite shows a better catalytic efficiency than tellurite with trxr1, whereas with trxr2, the catalyt ...201121397686
a role for zo-1 and plekha7 in recruiting paracingulin to tight and adherens junctions of epithelial cells.paracingulin is a 160-kda protein localized in the cytoplasmic region of epithelial tight and adherens junctions, where it regulates rhoa and rac1 activities by interacting with guanine nucleotide exchange factors. here, we investigate the molecular mechanisms that control the recruitment of paracingulin to cell-cell junctions. we show that paracingulin forms a complex with the tight junction protein zo-1, and the globular head domain of paracingulin interacts directly with zo-1 through an n-ter ...201121454477
in vivo activity of sapindus saponaria against azole-susceptible and -resistant human vaginal candida species.abstract:201121542936
analysis of environmental endocrine disrupting activities in wastewater treatment plant effluents using recombinant yeast assays incorporated with exogenous metabolic activation system.to measure the endocrine disrupting chemicals (edcs) in wastewater and evaluate the edcs removal efficiencies in the municipal wastewater treatment plants (wwtp).201121565684
pinta: a web server for network-based gene prioritization from expression data.pinta (available at http://www.esat.kuleuven.be/pinta/; this web site is free and open to all users and there is no login requirement) is a web resource for the prioritization of candidate genes based on the differential expression of their neighborhood in a genome-wide protein-protein interaction network. our strategy is meant for biological and medical researchers aiming at identifying novel disease genes using disease specific expression data. pinta supports both candidate gene prioritization ...201121602267
structure-activity relationships influencing lipid-induced changes in eif2α phosphorylation and cell viability in brin-bd11 cells.fatty acids influence the viability of eukaryotic cells differentially such that long chain saturated molecules are poorly tolerated, whereas unsaturated species are less detrimental and can be cytoprotective. the basis for these effects is unclear but studies in yeast imply that they reflect the spatial configuration of the molecules when incorporated into the er membrane. using brin-bd11 β-cells, we show that a wide range of unsaturated free fatty acids and their methyl-esters (having differin ...201121627968
yapsin 1 immunoreactivity in alpha cells of human pancreatic islets: implications for the processing of human proglucagon by mammalian aspartic proteases.yapsin 1 is an aspartic protease from saccharomyces cerevisiae and belongs to a class of aspartic proteases that demonstrate specificity for basic amino acids. it is capable of processing prohormone substrates at specific basic residue cleavage sites, similar to that of the prohormone convertases, to generate bioactive peptide hormones. an antibody raised against yapsin 1 was previously shown to immuno-stain endocrine cells of rat pituitary and brain as well as lysates from bovine pituitary secr ...201121632904
the wd40 repeat ptdins(3)p-binding protein epg-6 regulates progression of omegasomes to autophagosomes.ptdins(3)p plays critical roles in the autophagy pathway. however, little is known about how ptdins(3)p effectors act with autophagy proteins in autophagosome formation. here we identified an essential autophagy gene in c. elegans, epg-6, which encodes a wd40 repeat-containing protein with ptdins(3)p-binding activity. epg-6 directly interacts with atg-2. epg-6 and atg-2 regulate progression of omegasomes to autophagosomes, and their loss of function causes accumulation of enlarged early autophag ...201121802374
loco signaling pathway in longevity.despite the various roles of regulator of g protein signaling (rgs) protein in the g protein signaling pathway that have been defined, the function of rgs has not been characterized in longevity signaling pathways. we found that reduced expression of loco, a drosophila rgs protein, resulted in a longer lifespan of flies with stronger resistance to stress, higher mnsod activity and increased fat content. in contrast, overexpression of the loco gene shortened the fly lifespan significantly, lowere ...201121776417
The plasma membrane-associated GTPase Rin interacts with the dopamine transporter and is required for protein kinase C-regulated dopamine transporter trafficking.Dopaminergic signaling and plasticity are essential to numerous CNS functions and pathologies, including movement, cognition, and addiction. The amphetamine- and cocaine-sensitive dopamine (DA) transporter (DAT) tightly controls extracellular DA concentrations and half-life. DAT function and surface expression are not static but are dynamically modulated by membrane trafficking. We recently demonstrated that the DAT C terminus encodes a PKC-sensitive internalization signal that also suppresses b ...201121957239
schizosaccharomyces pombe, unlike saccharomyces cerevisiae, may not directly regulate nuclear-cytoplasmic transport of spliced trnas in response to nutrient availability.eukaryotic cells adapt to changes in nutrient levels by regulating key processes, such as gene transcription, ribosome biogenesis, and protein translation. several studies have shown that nuclear export of trnas is also regulated in saccharomyces cerevisiae and rat hepatoma h4iie cells during nutrient stress. however, recent studies suggest that nutrient stress does not affect nuclear trna export in several mammalian cell lines, including rat hepatoma h4iie. furthermore, in contrast to previous ...201122077425
Functional links between Aß toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast.Aß (beta-amyloid peptide) is an important contributor to Alzheimer's disease (AD). We modeled Aß toxicity in yeast by directing the peptide to the secretory pathway. A genome-wide screen for toxicity modifiers identified the yeast homolog of phosphatidylinositol binding clathrin assembly protein (PICALM) and other endocytic factors connected to AD whose relationship to Aß was previously unknown. The factors identified in yeast modified Aß toxicity in glutamatergic neurons of Caenorhabditis elega ...201122033521
glutamine analogs promote cytoophidium assembly in human and drosophila cells.ctp synthase is compartmentalized within a subcellular structure, termed the cytoophidium, in a range of organisms including bacteria, yeast, fruit fly and rat. here we show that ctp synthase is also compartmentalized into cytoophidia in human cells. surprisingly, the occurrence of cytoophidia in human cells increases upon treatment with a glutamine analog 6-diazo-5-oxo-l-norleucine (don), an inhibitor of glutamine-dependent enzymes including ctp synthase. experiments in flies confirmed that don ...201121930098
map4 mechanism that stabilizes mitochondrial permeability transition in hypoxia: microtubule enhancement and dynlt1 interaction with vdac1.mitochondrial membrane permeability has received considerable attention recently because of its key role in apoptosis and necrosis induced by physiological events such as hypoxia. the manner in which mitochondria interact with other molecules to regulate mitochondrial permeability and cell destiny remains elusive. previously we verified that hypoxia-induced phosphorylation of microtubule-associated protein 4 (map4) could lead to microtubules (mts) disruption. in this study, we established the hy ...201122164227
elevated levels of renal and circulating nop-7-associated 2 (nsa2) in rat and mouse models of diabetes, in mesangial cells in vitro and in patients with diabetic nephropathy.aims/hypothesis: we previously found that nop-7-associated 2 (nsa2), which is involved in ribosomal biogenesis in yeast and is a putative cell cycle regulator in mammalian cells, is elevated in the kidney of goto-kakizaki (gk) rat, a spontaneous model of type 2 diabetes. here we tested the hypothesis that elevated nsa2 is involved in diabetic nephropathy (dn). methods: we examined nsa2/nsa2 expression and nsa2 pr ...201122095236
preparation, characterization, and biological properties of β-glucans.β-glucans are soluble fibers with physiological functions, such as, interference with absorption of sugars and reduction of serum lipid levels. β-glucans are found in different species, such as, rhynchelytrum repens, lentinus edodes, grifola frondosa, tremella mesenterica, tremella aurantia, zea may, agaricus blazei, phellinus baummi, saccharomyces cerevisae (yeast), and agaricus blazei murell (mushroom). analysis of the fractions reveals the presence of arabinose, glucose, xylose, and traces of ...201122171300
ubiquitin ligase nedd4 promotes alpha-synuclein degradation by the endosomal-lysosomal pathway.α-synuclein is an abundant brain protein that binds to lipid membranes and is involved in the recycling of presynaptic vesicles. in parkinson disease, α-synuclein accumulates in intraneuronal inclusions often containing ubiquitin chains. here we show that the ubiquitin ligase nedd4, which functions in the endosomal-lysosomal pathway, robustly ubiquitinates α-synuclein, unlike ligases previously implicated in its degradation. purified nedd4 recognizes the carboxyl terminus of α-synuclein (residue ...201121953697
yeast-incorporated gallium promotes fracture healing by increasing callus bony area and improving trabecular microstructure on ovariectomized osteopenic rats.the purpose of this study was to analyze the impact of yeast-incorporated gallium on fracture healing in ovariectomized osteopenic rats. forty wistar female rats used were divided into three groups: sham-operated rats (sham), ovariectomized (ovx) rats, and ovx rats treated with yeast-bound gallium (yg). a standardized fracture-healing model with stable plate fixation was established for rat femoral. after 4-week stable fixation, animals were killed to prepare bones for micro-ct, biomechanical te ...201120446054
characterization of steroid receptor coactivator in sea urchin, strongylocentrotus nudus, and its involvement in embryonic development.ligand-bound nuclear receptors (nrs) recruit coactivators such as members of the p160 steroid receptor coactivator (src) family and cyclic amp responsive element binding protein (creb)-binding protein (cbp) to specific enhancer elements and activate target gene transcription. in the present study, we isolated a novel src from the sea urchin strongylocentrotus nudus (snsrc) by using the ligand-binding domain of retinoid x receptor as a bait in a yeast two-hybrid screening. the snsrc and vertebrat ...201120816915
ubiquitin-dependent proteasomal degradation of human liver cytochrome p450 2e1: identification of sites targeted for phosphorylation and ubiquitination.human liver cyp2e1 is a monotopic, endoplasmic reticulum-anchored cytochrome p450 responsible for the biotransformation of clinically relevant drugs, low molecular weight xenobiotics, carcinogens, and endogenous ketones. cyp2e1 substrate complexation converts it into a stable slow-turnover species degraded largely via autophagic lysosomal degradation. substrate decomplexation/withdrawal results in a fast turnover cyp2e1 species, putatively generated through its futile oxidative cycling, that inc ...201121209460
peptides and aptamers targeting hsp70: a novel approach for anticancer chemotherapy.the inhibition of heat shock protein 70 (hsp70) is an emerging strategy in cancer therapy. unfortunately, no specific inhibitors are clinically available. by yeast two-hybrid screening, we have identified multiple peptide aptamers that bind hsp70. when expressed in human tumor cells, two among these peptide aptamers-a8 and a17-which bind to the peptide-binding and the atp-binding domains of hsp70, respectively, specifically inhibited the chaperone activity, thereby increasing the cells' sensitiv ...201121224349
zinc-finger protein 90 negatively regulates neuron-restrictive silencer factor-mediated transcriptional repression of fetal cardiac genes.neuron-restrictive silencer factor (nrsf) is a zinc-finger transcription factor that binds to specific dna sequences (nrse) to repress transcription. by down-regulating the transcription of its target genes, nrsf contributes to the regulation of various biological processes, including neuronal differentiation, carcinogenesis and cardiovascular homeostasis. we previously reported that nrsf regulates expression of the cardiac fetal gene program, and that attenuation of nrsf-mediated repression con ...201121284946
nutrient stress does not cause retrograde transport of cytoplasmic trna to the nucleus in evolutionarily diverse organisms.intracellular trafficking of trna was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. this view has recently been challenged, however, by the discovery that trna can move retrograde from the cytoplasm back to the nucleus in saccharomyces cerevisiae and rat hepatoma h4iie cells during nutrient stress and in s. cerevisiae after intron-containing pre-trnas are spliced in the cytoplasm. contrary to studies reported, we prese ...201121289100
[effect of beta-carotene and carotene producing yeast phaffia rhodozyma on oxidative stress in rats treated with tetrachloromethane].supplementation of rats' diet with beta-carotene or biomass of carotene producing yeast phaffia rhodozyma caused a decrease of aminotransferases in the blood serum as well as a decrease of lipid peroxidation products and protein carbonil groups in the liver, brain and myocardium tissues of animals treated with tetrachloromethane. when compared to the control group the activity of superoxide dismutase, catalase and glutathione peroxidase in the liver of carotene fed rats were respectively 1.6, 2. ...201021328879
antiinflammatory, analgesic and antipyretic activities of mimusops elengi linn.in the present study, 70% ethanol extract of mimusops elengi linn. bark was assessed for antiinflammatory, analgesic and antipyretic activities in animals. the antiinflammatory activity of ethanol extract of mimusops elengi (200 mg/kg, p.o) was evaluated using carrageenan-induced paw edema and cotton pellet-induced granuloma models. analgesic effect was evaluated using acetic acid-induced writhing and eddy's hot plate models and antipyretic activity was assessed by brewer's yeast-induced pyrexia ...201021218059
prestin surface expression and activity are augmented by interaction with map1s, a microtubule-associated protein.prestin is a member of the slc26 family of anion transporters that is responsible for outer hair cell (ohc) electromotility. measures of voltage-evoked charge density (q(sp)) of prestin indicated that the protein is highly expressed in ohcs, with single cells expressing up to 10 million molecules within the lateral membrane. in contrast, charge density measures in transfected cells indicated that they express, at best, only a fifth as many proteins on their surface. we sought to determine whethe ...201020418376
synthesis, antimicrobial, and anti-inflammatory activities of novel 5-(1-adamantyl)-4-arylideneamino-3-mercapto-1,2,4-triazoles and related derivatives.the reaction of 5-(1-adamantyl)-4-amino-3-mercapto-1,2,4-triazole (5) with various aromatic aldehydes in ethanol or acetic acid yielded the corresponding 4-arylideneamino derivatives 6a-v. treatment of the 4-(2,6-difluoro- and dichlorobenzylideneamino) derivatives 6o and 6q with 1-substituted piperazines, and formaldehyde solution in ethanol afforded good yields of the corresponding 5-(1-adamantyl)-4-(2,6-dihalobenzylideneamino-2-(4-substituted-1-piperazinylmethyl)-1,2,4-triazoline-3-thiones 7a- ...201020428062
cryptococcus neoformans and cryptococcus gattii genes preferentially expressed during rat macrophage infection.cryptococcus neoformans and cryptococcus gattii are encapsulated yeast agents of cryptococcosis and facultative intracellular pathogens. the interaction of these yeasts with macrophages is essential for containing the infection. however, cryptococcus spp. overcome this initial host defense barrier using a unique pathogenic strategy involving intracellular replication and cytoplasmic accumulation of polysaccharide-containing vesicles. here, we employed representational difference analysis (rda) t ...201020302549
protogenin defines a transition stage during embryonic neurogenesis and prevents precocious neuronal differentiation.many ig superfamily members are expressed in the developing nervous system, but the functions of these molecules during neurogenesis are not all clear. here, we explore the expression and function of one of members of this superfamily, protogenin (prtg), in the developing nervous system. expression of prtg protein is strong in the neural tube of mouse embryos between embryonic days 7.75 and 9.5 but disappears after embryonic day 10.5 when the neural progenitor marker nestin expresses prominently ...201020335479
induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranes.cortical endoplasmic reticulum (cer) is a permanent feature of yeast cells but occurs transiently in most animal cell types. ist2p is a transmembrane protein that permanently localizes to the cer in yeast. when ist2 is expressed in mammalian cells, it induces abundant cer containing ist2. ist2 cytoplasmic c-terminal peptide is necessary and sufficient to induce cer. this peptide sequence resembles classic coat protein complex i (copi) coatomer protein-binding kkxx signals, and indeed the dimeriz ...201020351264
clp36 interacts with palladin in dorsal root ganglion neurons.clp36, a member of the alpha-actinin-associated lim protein (alp)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. however, the underlying molecular mechanisms are not known. in this study, we performed yeast two-hybrid screening of an e18 mouse whole-body cdna library with clp36 as the bait and isolated palladin as a clp36-binding protein. palladin is an actin-binding protein and it was shown to have a role in the extension of cortical neurons. a coimmu ...201020381583
regulation of m₃ muscarinic receptor expression and function by transmembrane protein 147.the m₃ muscarinic acetylcholine receptor (m3r) regulates many fundamental physiological functions. to identify novel m3r-interacting proteins, we used a recently developed yeast two-hybrid screen (split ubiquitin method) to detect interactions among membrane proteins. this screen led to the identification of many novel m3r-associated proteins, including the putative membrane protein transmembrane protein 147 (tmem147). the amino acid sequence of tmem147 is highly conserved among mammals, but its ...201021056967
antipyretic and antioxidant activities of 5-trifluoromethyl-4,5-dihydro-1h-pyrazoles in rats.the objective of this study was to determine the effect of eight 5-hydroxy-5-trifluoromethyl-4,5-dihydro-1h-1-carboxyamidepyrazoles (tfdps) on rat body temperature and baker's yeast-induced fever. tfdps or vehicle (5% tween 80 in 0.9% nacl, 5 ml/kg) were injected subcutaneously and rectal temperature was measured as a function of time in 28-day-old male wistar rats (n = 5-12 per group). antipyretic activity was determined in feverish animals injected with baker's yeast (saccharomyces cerevisiae ...201021140097
mammalian mthfd2l encodes a mitochondrial methylenetetrahydrofolate dehydrogenase isozyme expressed in adult tissues.previous studies in our laboratory showed that isolated, intact adult rat liver mitochondria are able to oxidize the 3-carbon of serine and the n-methyl carbon of sarcosine to formate without the addition of any other cofactors or substrates. conversion of these 1-carbon units to formate requires several folate-interconverting enzymes in mitochondria. the enzyme(s) responsible for conversion of 5,10-methylene-tetrahydrofolate (ch(2)-thf) to 10-formyl-thf in adult mammalian mitochondria are curre ...201021163947
protective role of raav-ndi1, serotype 5, in an acute mptp mouse parkinson's model.defects in mitochondrial proton-translocating nadh-quinone oxidoreductase (complex i) have been implicated in a number of acquired and hereditary diseases including leigh's syndrome and more recently parkinson's disease. a limited number of strategies have been attempted to repair the damaged complex i with little or no success. we have recently shown that the non-proton-pumping, internal nadh-ubiquinone oxidoreductase (ndi1) from saccharomyces cerevisiae (baker's yeast) can be successfully inse ...201021188192
antipyretic and antibacterial activity of chloranthus erectus (buch.-ham.) verdcourt leaf extract: a popular folk medicine of arunachal pradesh.the main objective of this work was to study the antipyretic and antibacterial activity of c. erectus (buch.-ham.) verdcourt leaf extract in an experimental albino rat model.201021206616
a heuristic model for paradoxical effects of biotin starvation on carbon metabolism genes in the presence of abundant glucose.we recently showed that in biotin starvation in yeast saccharomyces cerevisiae, nematode caenorhabditis elegans and rat rattus norvegicus, despite abundant glucose provision, the expression of genes for glucose utilization and lipogenesis were lowered, and for fatty acid β-oxidation and gluconeogenesis were raised, and glycolytic/fermentative flow was reduced. this work explored the mechanisms of these results. we show that they are associated with atp deficit and activation of the energy stress ...201020869286
rap1gap interacts with ret and suppresses gdnf-induced neurite outgrowth.glial cell line-derived neurotrophic factor (gdnf) was originally recognized for its ability to promote survival of midbrain dopaminergic neurons, but it has since been demonstrated to be crucial for the survival and differentiation of many neuronal subpopulations, including motor neurons, sympathetic neurons, sensory neurons and enteric neurons. to identify possible effectors or regulators of gdnf signaling, we performed a yeast two-hybrid screen using the intracellular domain of ret, the commo ...201020877310
identification of novel species-selective agonists of the g-protein-coupled receptor gpr35 that promote recruitment of β-arrestin-2 and activate gα13.the poorly characterized g-protein-coupled receptor gpr35 has been suggested as a potential exploratory target for the treatment of both metabolic disorders and hypertension. it has also been indicated to play an important role in immune modulation. a major impediment to validation of these concepts and further study of the role of this receptor has been a paucity of pharmacological tools that interact with gpr35. using a receptor-β-arrestin-2 interaction assay with both human and rat orthologue ...201020919992
the beta1 subunit of the na,k-atpase pump interacts with megalencephalic leucoencephalopathy with subcortical cysts protein 1 (mlc1) in brain astrocytes: new insights into mlc pathogenesis.megalencephalic leucoencephalopathy with subcortical cysts (mlc) is a rare congenital leucodystrophy caused by mutations in mlc1, a membrane protein of unknown function. mlc1 expression in astrocyte end-feet contacting blood vessels and meninges, along with brain swelling, fluid cysts and myelin vacuolation observed in mlc patients, suggests a possible role for mlc1 in the regulation of fluid and ion homeostasis and cellular volume changes. to identify mlc1 direct interactors and dissect the mol ...201020926452
applying genetics and molecular biology to the study of the human pathogen cryptococcus neoformans.the basidiomycete yeast crytococcus neoformans is a prominent human pathogen. it primarily infects immunocompromised individuals producing a meningoencephalitis that is lethal if untreated. recent advances in its genetics and molecular biology have made it a model system for understanding both the basidiomycota phylum and mechanisms of fungal pathogenesis. the relative ease of experimental manipulation coupled with the development of murine models for human disease allow for powerful studies in ...201020946836
the antibiotic gentamicin inhibits specific protein trafficking functions of the arf1/2 family of gtpases.gentamicin is a highly efficacious antibiotic against gram-negative bacteria. however, its usefulness in treating infections is compromised by its poorly understood renal toxicity. toxic effects are also seen in a variety of other organisms. while the yeast saccharomyces cerevisiae is relatively insensitive to gentamicin, mutations in any one of ∼20 genes cause a dramatic decrease in resistance. many of these genes encode proteins important for translation termination or specific protein-traffic ...201020956596
[lowered lipids efficiency of compound red yeast rice extract in rats with hyperlipidemia].to observe the effects of compound red yeast rice extract (cryre) on blood lipids, hemorheology and blood coagulant system in rats with hyperlipidemia.201020450054
antipyretic, analgesic, anti-inflammatory and antioxidant activities of two major chromenes from melicope lunu-ankenda.melicope lunu-ankenda (gaertn.) t.g. hartley is used in indian traditional medicine for fever, improving complexion and as a tonic. previous studies have isolated fungicidal, antifeedant, anti-inflammatory and immunomodulatory compounds from melicope lunu-ankenda. this study is aimed at the isolation and biological activity screening of potential molecules from the volatile oils and extracts of melicope lunu-ankenda in the light of traditional applications.201020457245
sparc interacts with ampk and regulates glut4 expression.amp-activated protein kinase (ampk) is a critical regulator of glucose metabolism. to elucidate the biochemical mechanisms by which ampk regulates glucose and fat metabolism, we conducted a yeast two-hybrid screen to identify its interacting partners. a yeast two-hybrid system was used to screen a mouse embryo cdna library for proteins able to bind mouse ampk alpha 1. we also demonstrated an endogenous interaction between ampk alpha 1 and its interacting partner by co-immunoprecipitation of the ...201020460104
prediction of functionally selective allosteric interactions at an m3 muscarinic acetylcholine receptor mutant using saccharomyces cerevisiae.saccharomyces cerevisiae is a tractable yeast species for expression and coupling of heterologous g protein-coupled receptors with the endogenous pheromone response pathway. although this platform has been used for ligand screening, no studies have probed its ability to predict novel pharmacology and functional selectivity of allosteric ligands. as a proof of concept, we expressed a rat m(3) muscarinic acetylcholine receptor (machr) bearing a mutation (k(7.32)e) recently identified to confer pos ...201020466821
carboxypeptidase e cytoplasmic tail mediates localization of synaptic vesicles to the pre-active zone in hypothalamic pre-synaptic terminals.how synaptic vesicles (svs) are localized to the pre-active zone (5-200 nm beneath the active zone) in the nerve terminal, which may represent the slow response sv pool, is not fully understood. electron microscopy revealed the number of svs located in the pre-active zone, was significantly decreased in hypothalamic neurons of carboxypeptidase e knockout (cpe-ko) mice compared with wild-type mice. additionally, we found k(+)-stimulated glutamate secretion from hypothalamic embryonic neurons was ...201020492353
transferrin fusion technology: a novel approach to prolonging biological half-life of insulinotropic peptides.fusion proteins made up of glucagon-like peptide 1 (glp-1) and exendin-4 (ex-4) fused to a nonglycosylated form of human transferrin (glp-1-tf or ex-4-tf) were produced and characterized. glp-1-tf activated the glp-1 receptor, was resistant to inactivation by peptidases, and had a half-life of approximately 2 days, compared with 1 to 2 min for native glp-1. glp-1-tf retained the acute, glucose-dependent insulin-secretory properties of native glp-1 in diabetic animals and had a profound effect on ...201020498254
study of anti-inflammatory, analgesic and antipyretic activities of seeds of hyoscyamus niger and isolation of a new coumarinolignan.a chemical and biological validation of the traditional use of hyoscyamus niger seeds as anti-inflammatory drug has been established. the methanolic extract of seeds of h. niger (mhn) was evaluated for its analgesic, anti-inflammatory and antipyretic activities in experimental animal models at different doses. mhn produced significant increase in hot plate reaction time, while decreasing writhing response in a dose-dependent manner indicating its analgesic activity. it was also effective in both ...201019720117
characterization of pceng2, a {beta}-1,3-endoglucanase homolog in pneumocystis carinii with activity in cell wall regulation.pneumocystis jirovecii pneumonia is an opportunistic fungal infection that causes severe respiratory impairment in immunocompromised patients. the viability of pneumocystis organisms is dependent on the cyst cell wall, a structural feature that is regulated by essential cell wall-associated enzymes. the formation of the glucan-rich cystic wall has been previously characterized, but glucan degradation in the organism-specifically, degradation during trophic excystment-is not yet fully understood. ...201019783787
ranbpm regulates the progression of neuronal precursors through m-phase at the surface of the neocortical ventricular zone.many of the mitoses that produce pyramidal neurons in neocortex occur at the dorsolateral surface of the lateral ventricles in the embryo. ranbpm was found in a yeast two-hybrid screen to potentially interact with citron kinase (citk), a protein shown previously to localize to the surface of the lateral ventricles and to be essential to neurogenic mitoses. similar to its localization in epithelia, ranbpm protein is concentrated at the adherens junctions in developing neocortex. the biochemical i ...201019790105
inhibition of hepatic carnitine palmitoyl-transferase i (cpt ia) by valproyl-coa as a possible mechanism of valproate-induced steatosis.carnitine palmitoyl-transferase i (cpt i) catalyses the synthesis of long-chain (lc)-acylcarnitines from lc-acyl-coa esters. it is the rate-limiting enzyme of mitochondrial fatty acid beta-oxidation (fao) pathway and its activity is regulated by malonyl-coa. the antiepileptic drug valproic acid (vpa) is a branched chain fatty acid that is activated to the respective coa ester in the intra- and extra-mitochondrial compartments. this drug has been associated with a clear inhibition of mitochondria ...201019854160
comparison of the therapeutic effects of yeast-incorporated gallium with those of inorganic gallium on ovariectomized osteopenic rats.the purpose of this study was to compare the effect and toxicity of yeast-incorporated gallium (yg) with inorganic gallium (gallium nitrate, ig) in preventing osteoporosis in ovariectomized (ovx) wistar female rats. after the rats were treated orally with yg and ig respectively, the urine calcium, plasma calcium, plasma phosphorus, bone mineral content, mechanical testing, and the mass of uterus, thymus, and body were examined. treatments were performed over an 8-week period. both yg and ig have ...201019652924
quantitative proteomics by metabolic labeling of model organisms.in the biological sciences, model organisms have been used for many decades and have enabled the gathering of a large proportion of our present day knowledge of basic biological processes and their derailments in disease. although in many of these studies using model organisms, the focus has primarily been on genetics and genomics approaches, it is important that methods become available to extend this to the relevant protein level. mass spectrometry-based proteomics is increasingly becoming the ...201019955089
association of whirlin with cav1.3 (alpha1d) channels in photoreceptors, defining a novel member of the usher protein network.usher syndrome is the most common form of hereditary deaf-blindness. it is both clinically and genetically heterogeneous. the ush2d protein whirlin interacts via its pdz domains with other usher-associated proteins containing a c-terminal type i pdz-binding motif. these proteins co-localize with whirlin at the region of the connecting cilium and at the synapse of photoreceptor cells. this study was undertaken to identify novel, usher syndrome-associated, interacting partners of whirlin and there ...201019959638
glucuronide production by whole-cell biotransformation using genetically engineered fission yeast schizosaccharomyces pombe.drug metabolites generated by udp glycosyltransferases (ugts) are needed for drug development and toxicity studies, especially in the context of safety testing of metabolites during drug development. because chemical metabolite synthesis can be arduous, various biological approaches have been developed; however, no whole-cell biotransformation with recombinant microbes that express human ugts was yet achieved. in this study we expressed human udp glucose-6-dehydrogenase together with several hum ...201020008039
penetrating cation/fatty acid anion pair as a mitochondria-targeted protonophore.a unique phenomenon of mitochondria-targeted protonophores is described. it consists in a transmembrane h(+)-conducting fatty acid cycling mediated by penetrating cations such as 10-(6'-plastoquinonyl)decyltriphenylphosphonium (skq1) or dodecyltriphenylphosphonium (c(12)tpp). the phenomenon has been modeled by molecular dynamics and directly proved by experiments on bilayer planar phospholipid membrane, liposomes, isolated mitochondria, and yeast cells. in bilayer planar phospholipid membrane, t ...201020080732
ceramide and activated bax act synergistically to permeabilize the mitochondrial outer membrane.a critical step in apoptosis is mitochondrial outer membrane permeabilization (momp), releasing proteins critical to downstream events. while the regulation of this process by bcl-2 family proteins is known, the role of ceramide, which is known to be involved at the mitochondrial level, is not well-understood. here, we demonstrate that bax and ceramide induce momp synergistically. experiments were performed on mitochondria isolated from both rat liver and yeast (lack mammalian apoptotic machiner ...201020101465
applying hsp104 to protein-misfolding disorders.hsp104, a hexameric aaa+ atpase found in yeast, transduces energy from cycles of atp binding and hydrolysis to resolve disordered protein aggregates and cross-beta amyloid conformers. these disaggregation activities are often co-ordinated by the hsp70 chaperone system and confer considerable selective advantages. first, renaturation of aggregated conformers by hsp104 is critical for yeast survival after various environmental stresses. second, amyloid remodeling by hsp104 enables yeast to exploit ...201020130674
modulation of myocardin function by the ubiquitin e3 ligase ubr5.fully differentiated mature smooth muscle cells (smcs) are characterized by the presence of a unique repertoire of smooth muscle-specific proteins. although previous studies have shown myocardin to be a critical transcription factor for stimulating expression of smooth muscle-specific genes, the mechanisms regulating myocardin activity are still poorly understood. we used a yeast two-hybrid screen with myocardin as bait to search for factors that may regulate the transcriptional activity of the ...201020167605
acute regulation of renal na+/h+ exchanger nhe3 by dopamine: role of protein phosphatase 2a.nephrogenic dopamine is a potent natriuretic paracrine/autocrine hormone that is central for mammalian sodium homeostasis. in the renal proximal tubule, dopamine induces natriuresis partly via inhibition of the sodium/proton exchanger nhe3. the signal transduction pathways and mechanisms by which dopamine inhibits nhe3 are complex and incompletely understood. this manuscript describes the role of the serine/threonine protein phosphatase 2a (pp2a) in the regulation of nhe3 by dopamine. the pp2a r ...201020181665
mitochondria-targeted cytochrome p450 2e1 induces oxidative damage and augments alcohol-mediated oxidative stress.the ethanol-inducible cytochrome p450 2e1 (cyp2e1) is also induced under different pathological and physiological conditions. studies including ours have shown that cyp2e1 is bimodally targeted to both the endoplasmic reticulum (microsomes) (mc cyp2e1) and mitochondria (mt cyp2e1). in this study we investigated the role of mtcyp2e1 in ethanol-mediated oxidative stress in stable cell lines expressing predominantly mt cyp2e1 or mc cyp2e1. the er+ mutation (a2l, a9l), which increases the affinity o ...201020529841
alpha4 phosphoprotein interacts with edd e3 ubiquitin ligase and poly(a)-binding protein.mammalian alpha4 phosphoprotein, the homolog of yeast tap42, is a component of the mammalian target-of-rapamycin (mtor) pathway that regulates ribogenesis, the initiation of translation, and cell-cycle progression. alpha4 is known to interact with the catalytic subunit of protein phosphatase 2a (pp2ac) and to regulate pp2a activity. using alpha4 as bait in yeast two-hybrid screening of a human k562 erythroleukemia cdna library, edd (e3 isolated by differential display) e3 ubiquitin ligase was id ...201020544796
the production, characterisation and enhanced pharmacokinetics of scfv-albumin fusions expressed in saccharomyces cerevisiae.an expression system is described for the production of monomeric scfvs and scfv antibody fragments genetically fused to human albumin (at either the n- or c-terminus or both). based upon strains of saccharomyces cerevisiae originally developed for the production of a recombinant human albumin (recombumin) this system has delivered high levels of secreted product into the supernatant of shake flask and high cell density fed-batch fermentations. specific binding to the corresponding ligand was de ...201020546898
estrogenic pyrethroid pesticides regulate expression of estrogen receptor transcripts in mouse sertoli cells differently from 17beta-estradiol.studies suggested that exposure to agricultural pesticides may affect male fertility. pyrethroids are widely used pesticides due to their insecticidal potency and low mammalian toxicity. a recombinant yeast assay system incorporating the human alpha-estrogen receptor was used to analyze the estrogenicity of a range of readily available pyrethroid pesticides. the commercial product ripcord plus showed estrogenic activity by this assay. to determine whether pyrethroid compounds might exert an effe ...201020574910
preliminary safety assessment of yarrowia lipolytica extracellular lipase: results of acute and 28-day repeated dose oral toxicity studies in rats.interest in extracellular lipase sourced from the non conventional yeast yarrowia lipolytica has increased over the last decade. the enzyme was recently suggested as a good candidate for pancreatic exocrine insufficiency treatment. however, there is still a lack of oral safety evaluation data. in this work, we conducted acute and 28-day repeated dose toxicity studies in rats. both male and female rats were first orally treated with fungal lipase at either single or repeated doses. the results de ...201020609427
kbp interacts with scg10, linking goldberg-shprintzen syndrome to microtubule dynamics and neuronal differentiation.goldberg-shprintzen syndrome (goshs) is a rare clinical disorder characterized by central and enteric nervous system defects. this syndrome is caused by inactivating mutations in the kinesin binding protein (kbp) gene, which encodes a protein of which the precise function is largely unclear. we show that kbp expression is up-regulated during neuronal development in mouse cortical neurons. moreover, kbp-depleted pc12 cells were defective in nerve growth factor-induced differentiation and neurite ...201020621975
successful amelioration of mitochondrial optic neuropathy using the yeast ndi1 gene in a rat animal model.leber's hereditary optic neuropathy (lhon) is a maternally inherited disorder with point mutations in mitochondrial dna which result in loss of vision in young adults. the majority of mutations reported to date are within the genes encoding the subunits of the mitochondrial nadh-quinone oxidoreductase, complex i. establishment of animal models of lhon should help elucidate mechanism of the disease and could be utilized for possible development of therapeutic strategies.201020628600
glucocorticoid protection of oligodendrocytes against excitotoxin involving hypoxia-inducible factor-1alpha in a cell-type-specific manner.glucocorticoids are commonly used in treating diseases with white matter lesions, including demyelinating diseases and spinal cord injury (sci). however, glucocorticoids are ineffective in gray matter injuries, such as head injury and stroke. the differential glucocorticoid effects in white and gray matter injuries are unclear. we report here a novel mechanism of methylprednisolone (mp), a synthetic glucocorticoid widely used for treating multiple sclerosis and sci, in protecting oligodendrocyte ...201020631191
the role of autophagy during development in higher eukaryotes.autophagy is a lysosome-mediated degradation pathway used by eukaryotes to recycle cytosolic components in both basal and stress conditions. several genes have been described as regulators of autophagy, many of them being evolutionarily conserved from yeast to mammals. the study of autophagy-defective model systems has made it possible to highlight the importance of correctly functioning autophagic machinery in the development of invertebrates as, for example, during the complex events of fly an ...201020633243
pneumocystis carinii expresses an active rtt109 histone acetyltransferase.pneumocystis (pc) remains a severe cause of pneumonia in immune compromised hosts. identification of specific targets present in pc, but lacking in humans, is paramount to developing new means to treat this infection. one such potential protein is rtt109, a unique histone acetyltransferase (hat) that acetylates h3 lysine 56 (h3-k56) in yeast and is required for dna replication. sequence homologues of rtt109 are present in other fungi, but are absent in mammals, making it a potential pan-specific ...201020656950
the absence of genotoxicity of sucralose.sucralose is a non-nutritive sweetener that is approximately 600 times sweeter than table sugar. it is currently approved for use in over 80 countries. evidence from chronic studies demonstrates that this compound is not carcinogenic. this report summarizes the results of genotoxicity studies that were part of the original safety assessment of sucralose-conducted early in the safety investigation and shared with regulatory agencies around the world. studies included the ames (salmonella typhimur ...201020691239
evaluation of aqueous extract of felicia muricata leaves for anti-inflammatory, antinociceptive, and antipyretic activities.felicia muricata thunb. (nees) (asteraceae) leaves are used in folklore medicine of south africa as an oral remedy for pain and inflammation. however, the efficacy of the plant part is yet to be validated with scientific experiments.201020731550
marked amplification and diversification of products of ras genes from rat brain, rab gtpases, in the ciliates tetrahymena thermophila and paramecium tetraurelia.small gtpase rab (products of ras genes from rat brain) is a widely conserved molecular switch among eukaryotes and regulates membrane trafficking pathways. it is generally considered that the number of rab encoded in the genome correlates with multicellularity; however, we found that unicellular ciliates tetrahymena thermophila (tt) and paramecium tetraurelia (pt) possess many more rab genes in their genome than the 64 hsrab genes in the human genome. we succeeded in isolating 86 cdna clones of ...201020738463
biotin starvation with adequate glucose provision causes paradoxical changes in fuel metabolism gene expression similar in rat (rattus norvegicus), nematode (caenorhabditis elegans) and yeast (saccharomyces cerevisiae).biotin affects the genetic expression of several glucose metabolism enzymes, besides being a cofactor of carboxylases. to explore how extensively biotin affects the expression of carbon metabolism genes, we studied the effects of biotin starvation and replenishment in 3 distantly related eukaryotes: yeast saccharomyces cerevisiae, nematode caenorhabditis elegans and rat rattus norvegicus.201020798549
antioxidant rich grape pomace extract suppresses postprandial hyperglycemia in diabetic mice by specifically inhibiting alpha-glucosidase.abstract:201020799969
safety assessment of epa-rich oil produced from yeast: results of a 90-day subchronic toxicity study.the safety of eicosapentaenoic acid (epa) oil produced from genetically modified yarrowia lipolytica yeast was evaluated following 90 days of exposure. groups of rats received 0 (olive oil), 98, 488, or 976 mg epa/kg/day, or gras fish oil or deionized water by oral gavage. rats were evaluated for in-life, neurobehavioral, anatomic and clinical pathology parameters. lower serum cholesterol (total and non-hdl) was observed in medium and high epa and fish oil groups. lower hdl was observed in high ...201020804805
optineurin and its mutants: molecules associated with some forms of glaucoma.optineurin is a gene (optn) associated with normal-tension glaucoma and primary open-angle glaucoma. the aim of our study is to understand the functions of the protein optineurin in retinal ganglion cells, and the modifications that come about in its functions when mutations occur in its sequence.200919672125
the inactivation of the sortilin gene leads to a partial disruption of prosaposin trafficking to the lysosomes.lysosomes are intracellular organelles which contain enzymes and activator proteins involved in the digestion and recycling of a variety of cellular and extracellular substances. we have identified a novel sorting receptor, sortilin, which is involved in the lysosomal trafficking of the sphingolipid activator proteins, prosaposin and gm(2)ap, and the soluble hydrolases cathepsin d, cathepsin h, and acid sphingomyelinase. sortilin belongs to a growing family of receptors with homology to the yeas ...200919732768
uncoupling protein 1 inhibition by purine nucleotides is under the control of the endogenous ubiquinone redox state.we studied non-esterified fatty acid-induced uncoupling of heterologously expressed rat ucp1 (uncoupling protein 1) in yeast mitochondria, as well as ucp1 in rat bat (brown adipose tissue) mitochondria. the proton-conductance curves and the relationship between the ubiquinone reduction level and membrane potential were determined in non-phosphorylating bat and yeast mitochondria. the adp/o method was applied to determine the adp phosphorylation rate and the relationship between the ubiquinone re ...200919747168
yeast ste23p shares functional similarities with mammalian insulin-degrading enzymes.the s. cerevisiae genome encodes two m16a enzymes: axl1p and ste23p. of the two, ste23p shares significantly higher sequence identity with m16a enzymes from other species, including mammalian insulin-degrading enzymes (ides). in this study, recombinant ste23p and r. norvegicus ide (rnide) were isolated from e. coli, and their enzymatic properties compared. ste23p was found to cleave established rnide substrates, including the amyloid-beta peptide (abeta1-40) and insulin b-chain. a novel internal ...200919750477
efficient synthesis of functional isoprenoids from acetoacetate through metabolic pathway-engineered escherichia coli.we show here an efficient synthesis system of isoprenoids from acetoacetate as the main substrate. we expressed in escherichia coli a streptomyces mevalonate pathway gene cluster starting from hmg-coa synthase and including isopentenyl diphosphate isomerase (idi) type 2 gene and the yeast idi type 1 and rat acetoacetate-coa ligase (aacl) genes. when the alpha-humulene synthase (zss1) gene of shampoo ginger was expressed in this transformant, the resultant e. coli produced 958 mug/ml culture of a ...200918836713
modulation of experimental osteoporosis in rats by the antioxidant beverage effective microorganism-x (em-x).osteoporosis is a disease of aging associated with bone loss that often occurs without symptoms until microarchitectural deterioration becomes so significant that bone fracture occurs. the effective microorganism-x (em-x) is an antioxidant beverage derived from ferment of unpolished rice, sea weeds and papaya with effective microorganisms of lactic acid bacteria, yeast and photosynthetic bacteria (containing minerals, alpha-tocopherol, lycopene, ubiquinone, saponin and flavonoids). the levels of ...200918930627
synthesis and pharmacological evaluation of a novel series of 5-(substituted)aryl-3-(3-coumarinyl)-1-phenyl-2-pyrazolines as novel anti-inflammatory and analgesic agents.a novel series of 5-(substituted)aryl-3-(3-coumarinyl)-1-phenyl-2-pyrazolines (3a-l) were synthesized by reacting various substituted 3-aryl-1-(3-coumarinyl)propan-1-ones (2a-l) with phenylhydrazine in the presence of hot pyridine. structures of all new synthesized compounds were characterized on the basis of elemental analysis and spectral data (ir, (1)h nmr and (13)c nmr). the title compounds were screened for in vivo anti-inflammatory and analgesic activities at a dose of 200 mg/kg b.w. among ...200918986738
structural and functional differences between krit1a and krit1b isoforms: a framework for understanding ccm pathogenesis.krit1 is a disease gene responsible for cerebral cavernous malformations (ccm). it encodes for a protein containing distinct protein-protein interaction domains, including three npxy/f motifs and a ferm domain. previously, we isolated krit1b, an isoform characterized by the alternative splicing of the 15th coding exon and suspected to cause ccm when abnormally expressed. combining homology modeling and docking methods of protein-structure and ligand binding prediction with the yeast two-hybrid a ...200918992740
activation of protein kinase czeta increases oat1 (slc22a6)- and oat3 (slc22a8)-mediated transport.organic anion transporters (oats) play a pivotal role in the clearance of small organic anions by the kidney, yet little is known about how their activity is regulated. a yeast two-hybrid assay was used to identify putative oat3-associated proteins in the kidney. atypical protein kinase czeta (pkczeta) was shown to bind to oat3. binding was confirmed in immunoprecipitation assays. the oat3/pkczeta interaction was investigated in rodent renal cortical slices from fasted animals. insulin, an upstr ...200919028678
protein sycp2 provides a link between transverse filaments and lateral elements of mammalian synaptonemal complexes.synaptonemal complexes (scs) are evolutionarily conserved meiosis-specific nuclear structures critically involved in synapsis, recombination, and segregation of homologous chromosomes. scs are proteinaceous structures composed of (a) two lateral elements (les), to which the chromatin of the homologs is attached, (b) numerous transverse filaments (tfs) that link the les, and (c) a central element (ce). major protein components of mammalian scs are the tf protein sycp1 and the le proteins sycp2 an ...200919034475
a yeast display immunoprecipitation method for efficient isolation and characterization of antigens.yeast antibody display has found a wide variety of applications including antibody affinity maturation, epitope mapping, and library screening. here we report a yeast display immunoprecipitation (ydip) technique that employs yeast cells displaying single-chain antibody fragments (scfv) on their surface as affinity capture reagents to isolate and characterize antigens. we show that displayed single-chain antibody fragments are active in a variety of detergent solutions commonly used for immunopre ...200919041873
homology modeling of human transketolase: description of critical sites useful for drug design and study of the cofactor binding mode.transketolase, the most critical enzyme of the non-oxidative branch of the pentose phosphate pathway, has been reported as a new target protein for cancer research. however, since the crystal structure of human transketolase is unknown, no structure-based methods can be used to identify new inhibitors. we performed homology modeling of human transketolase using the crystal structure of yeast as a template, and then refined the model through molecular dynamics simulations. based on the resulting ...200919111488
studies on the analgesic, anti-inflammatory and antipyretic effects of parquetina nigrescens leaf extract.parquetina nigrescens is a shrub that is commonly used in different parts of west africa for the treatment of several ailments which includes pain, fever and inflammatory conditions.200919111918
signalling pathway involving gulp, mapk and rac1 for sr-bi-induced phagocytosis of apoptotic cells.class b scavenger receptor type i (sr-bi) is a phosphatidylserine (ps)-recognizing receptor of testicular sertoli cells responsible for the phagocytosis of spermatogenic cells undergoing apoptosis. here, we determined signal mediators that compose a signalling pathway for sr-bi-induced phagocytosis. results of a yeast two-hybrid analysis and a cell-free binding assay indicated that sr-bi binds to engulfment adapter protein (gulp) using the c-terminal intracellular domain. a co-immunoprecipitatio ...200919122200
expression of two human acyl-coa:diacylglycerol acyltransferase isozymes in yeast and selectivity of microbial inhibitors toward the isozymes.two isozymes for human acyl-coenzyme a:diacylglycerol acyltransferase (dgat), dgat1 and dgat2, were independently expressed in dgat-deficient saccharomyces cerevisiae to establish dgat1- and dgat2-s. cerevisiae. the selectivity of dgat inhibitors of natural origin towards the isozymes was assessed in enzyme assays using the microsomal fractions prepared from dgat1- and dgat2-s. cerevisiae. amidepsines and xanthohumol inhibited dgat1 and dgat2 with similar potency, whereas roselipins were found t ...200919132064
self-association of transmembrane domain 2 (tm2), but not tm1, in carnitine palmitoyltransferase 1a: role of gxxxg(a) motifs.carnitine palmitoyltransferase 1 (cpt1) controls the rate of entry of long-chain fatty acids into the mitochondrial matrix for beta-oxidation and has been reported to exist as an oligomer. we have investigated the in vivo oligomerization of full-length rat cpt1a (rcpt1a) along with those of the n-terminal truncation/deletion mutants delta(1-82), delta(1-18), and delta(19-30) expressed in yeast mitochondria. the data indicate that in liver mitochondria in vivo cpt1a exists as a hexamer but that d ...200919136561
Refresh
firstprev
12345678910...
nextlast
Displaying items 1 - 100 of 2172