Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| complete genome sequences of brucella melitensis strains m28 and m5-90, with different virulence backgrounds. | brucella melitensis is a gram-negative coccobacillus bacteria belonging to the alphaproteobacteria subclass. it is an important zoonotic pathogen that causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. the b. melitensis strain m5-90, a live attenuated vaccine cultured from the b. melitensis virulent strain m28, has been an effective tool to control brucellosis in goats and sheep in china. here we report the complete genome sequences of b. melitensis m28 and m5-90, strai ... | 2011 | 21478357 |
| [genetic characterization of the brucella melitensis isolates from mongolia, russia, and azerbaijan]. | the goal of this work was to provide comparative genetic characterization of the human and animal brucella melitensis isolates from mongolia, russia and azerbaijan using current molecular-genetic typing methods. | 2011 | 21786630 |
| Immunoproteomic analysis of Brucella melitensis and identification of a new immunogenic candidate protein for the development of brucellosis subunit vaccine. | In order to screen immunogenic candidate antigens for the development of a brucellosis subunit vaccine, an immunoproteomic assay was used to identify immunogenic proteins from Brucella melitensis 16 M soluble proteins. In this study, a total of 56 immunodominant proteins were identified from the two-dimensional electrophoresis immunoblot profiles by liquid chromatography tandem mass spectrometry (LC-MS/MS). Two proteins of interest, riboflavin synthase alpha chain (RS-a) and Loraine synthase (LS ... | 2011 | 21943783 |
| control and eradication of brucella melitensis infection in sheep and goats. | brucella melitensis is the main etiological agent of brucellosis in sheep and goats, and is also the main agent responsible for human brucellosis, a predominantly occupational disease related to professions in direct contact with livestock. as there is currently no viable method of preventing human brucellosis to safeguard people attention must be directed toward effectively controlling the disease in sheep and goats. this review focuses on the different strategies in different socioeconomic and ... | 2011 | 21215893 |
| expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of brucella melitensis m5-90: a potential antigen for differential serodiagnosis of brucellosis in sheep and goats. | antibodies produced in animals vaccinated using live attenuated vaccines against brucella spp. are indistinguishable using current conventional serological tests from those produced in infected animals. one potential approach is to develop marker vaccines in which specific genes have been deleted from parental vaccine strains that show good immunogenicity and vaccine efficacy. corresponding methods of detection for antibodies raised by the marker vaccine should also be developed. a specific frag ... | 2011 | 21446957 |
| isolation of brucella melitensis from a rb51-vaccinated seronegative goat. | the purpose of this study is to determine the etiology of abortions presented in a goat herd declared as free of brucellosis and vaccinated with rb51 located in mexico. the serological diagnosis of brucellosis in 33 animals was performed. the study included three goats that aborted in the last third of gestation and 15 goats that gave birth normally; samples of milk and vaginal exudate were subjected to bacteriological study. all animals were negative for serological diagnosis, and isolation of ... | 2011 | 21455694 |
| Brucella melitensis Survival During Manufacture of Ripened Goat Cheese at Two Temperatures. | Abstract The aim of the current work was to assess the influence of two temperatures, 4°C and 24°C, on pH and water activity and their association with Brucella melitensis survival during the traditional manufacture of ripened goat cheese. Raw milk from a brucellosis-free goat herd was used for the manufacture of ripened cheese. The cheese was inoculated with 5×10(9) of the B. melitensis 16M strain during the tempering stage. The cheeses were matured for 5, 20, and 50 days at both temperatures ... | 2011 | 22129442 |
| large scale immune profiling of infected humans and goats reveals differential recognition of brucella melitensis antigens. | brucellosis is a widespread zoonotic disease that is also a potential agent of bioterrorism. current serological assays to diagnose human brucellosis in clinical settings are based on detection of agglutinating anti-lps antibodies. to better understand the universe of antibody responses that develop after b. melitensis infection, a protein microarray was fabricated containing 1,406 predicted b. melitensis proteins. the array was probed with sera from experimentally infected goats and naturally i ... | 2010 | 20454614 |
| epidemiological characteristics of brucellosis in sheep and goats in bulgaria: 2005-2008. | (full text is available at http://www.manu.edu.mk/prilozi). aim: to analyse and present the epidemiological and epizootological situation of brucellosis in humans, sheep and goats caused by brucella melitensis. methods: the veterinary health services analyzsd epidemiological reports, as well as other official documents, in relation to the epidemiology and the measures undertaken for control of brucellosis in bulgaria, 2005-08. results: in bulgaria animal infections caused by brucella melitensis ... | 2010 | 20703183 |
| diagnostic validation of ovine and caprine brucellosis using serum- and milk-elisa. | (full text is available at http://www.manu.edu.mk/prilozi). aim: to introduce and validate the new method of diagnosing ovine and caprine brucellosis in a rapid, accurate and inexpensive manner by using i-elisa (serum/milk) technique. methods: serum and milk samples from brucella rb and cft negative (n = 881) and positive (n = 755) animals were used. standardization of tests was through the bommeli elisa-besw (brucella bang) standard and our institute's (mkd) working standards (positive serum an ... | 2010 | 20703186 |
| evaluation of the fluorescence polarization assay (fpa) for diagnosis of brucella melitensis infection of goats in argentina. | (full text is available at http://www.manu.edu.mk/prilozi). aim: determine the optimal cut-off and the use of fluorescence polarization assay (fpa) to improve the detection of brucellosis in individual goats in argentina. methods: sera from 96 goats from a flock with abortion due to b. melitensis biovar 1 were used to assess the efficacy of the fpa to detect brucellosis in goats. fpa results were compared with those of the buffered antigen plate agglutination test (bpat) confirmed by seroaggluti ... | 2010 | 20703188 |
| control and eradication strategies for brucella melitensis infection in sheep and goats. | (full text is available at http://www.manu.edu.mk/prilozi). aim: to describe the various strategies for the control and eradication of b. melitensis infection in sheep and goats. methods: the advantages and drawbacks of these strategies are discussed on the basis of the author's personal experience and a revision of the relevant literature. results: vaccination programmes in various combinations can be applied either to decrease the prevalence of infection in the animal population or, when combi ... | 2010 | 20703189 |
| serological, bacteriological, and molecular diagnosis of brucellosis in domestic animals in croatia. | to present the surveillance data on brucella melitensis, b. suis, and b. ovis infection in cattle, sheep, goats, and swine in croatia obtained in 2008 by serological, bacteriological, and molecular methods for diagnostics of brucellosis in domestic animals. | 2010 | 20718085 |
| ovine and caprine brucellosis (brucella melitensis) in aborted animals in jordanian sheep and goat flocks. | two hundred and fifty five biological samples were collected from 188 animals (81 sheep and 107 goats) during the lambing season from september 2009 to april 2010 from the mafraq region of jordan. sampled animals belonged to 93 sheep and goat flocks that had abortion cases in the region. one hundred and seven (41.9%) biological samples were positive for the omp2 primers that were able to identify all brucella species in the collected samples which were obtained from 86 aborted animals (86/188 = ... | 2010 | 21052561 |
| recombinant omp28 antigen-based indirect elisa for serodiagnosis of bovine brucellosis. | brucellosis is a zoonosis of both public health and economic importance in many developing countries including india. early detection and segregation of the infected animals are important in order to control the disease. serodiagnostic tests for brucellosis is mainly based on detection of antibodies developed against lipopolysaccharide (lps) component of cell. in this study we evaluated a protein antigen, 28 kda outer membrane protein (omp28), of brucella melitensis as an alternative to lps. rec ... | 2010 | 20025961 |
| spatial distribution and risk factors of brucellosis in iberian wild ungulates. | the role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. the aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several iberian wild ungulates. | 2010 | 20205703 |
| evaluating the virulence of a brucella melitensis hemagglutinin gene in the caprine model. | with the completion of the genomic sequence of brucella melitensis 16m, a putative hemagglutinin gene was identified which is present in 16m and absent in brucella abortus. the possibility of this hemagglutinin being a potential virulence factor was evaluated via gene replacement in b. melitensis yielding 16mδe and expression in trans in b. abortus 2308-qae. utilizing the caprine brucellosis model, colonization and pathogenesis studies were performed to evaluate these strains. b. melitensis 16m ... | 2010 | 20362205 |
| bilateral knee arthroplasty infection due to brucella melitensis: a rare pathology? | 2009 | 19157951 | |
| genotyping of brucella melitensis by rpob gene analysis and re-evaluation of conventional serotyping method. | in turkey, where brucellosis is endemic, a comparison of conventional and molecular genotyping methods has not been published to date. in this study, we investigated the efficacy of single nucleotide polymorphism (snp) analysis of rpob gene in the genotyping of brucella melitensis strains by sequencing. in light of the molecular genotyping method available now in turkey, the adequacy of serological typing alone should be re-evaluated as a tool for epidemiologic studies of b. melitensis. | 2009 | 19305062 |
| establishment of systemic brucella melitensis infection through the digestive tract requires urease, the type iv secretion system, and lipopolysaccharide o antigen. | human brucellosis is caused mainly by brucella melitensis, which is often acquired by ingesting contaminated goat or sheep milk and cheese. bacterial factors required for food-borne infection of humans by b. melitensis are poorly understood. in this study, a mouse model of oral infection was characterized to assess the roles of urease, the virb type iv secretion system, and lipopolysaccharide for establishing infection through the digestive tract. b. melitensis strain 16m was consistently recove ... | 2009 | 19651862 |
| diagnosis of placental pathogens in small ruminants by immunohistochemistry and pcr on paraffin-embedded samples. | a histological study was carried out on 58 formalin-fixed and paraffin-embedded samples of placenta from sheep and goats that had aborted, and the placental lesions were graded. sequential histological sections of each cotyledon were then immunostained with specific antibodies and used for pcr detection of chlamydophila abortus, coxiella burnetii, salmonella abortusovis, brucella melitensis, listeria monocytogenes and toxoplasma gondii. most of the cotyledons showed different degrees of placenti ... | 2009 | 19666916 |
| molecular epidemiology of brucella genotypes in patients at a major hospital in central peru. | the multiple-locus variable-number repeat analysis of 90 human brucella melitensis isolates from a large urban area in central peru revealed variations at 4 (bruce07, bruce09, bruce18, and bruce42) out of 16 loci investigated, of which 1 (bruce42) also is used for species identification. ten genotypes were identified, separated by the number of bruce42 repeats into two groups that may have distinct phenotypic characteristics. whereas genotypes with five or six bruce42 repeats were cultured mainl ... | 2009 | 19656979 |
| prosthetic valve endocarditis due to brucella: successful outcome with a combined strategy. | infective endocarditis is a serious heart disease that can affect native and prosthetic valves. staphylococci and streptococci are the etiological agents in almost 80% of cases. human brucellosis is a multiple organ disease often transmitted via contaminated, unpasteurized goat milk and cheese. brucella endocarditis is a rare complication associated with elevated morbidity and mortality rates; it can affect native or, even more rarely, prosthetic valves. in this report, we present the successful ... | 2009 | 19283882 |
| mlva genotyping of human brucella isolates from peru. | recent human brucella melitensis isolates from peru were genotyped by multiple locus variable number repeat analysis. all 24 isolates originated from hospitalized patients living in the central part of peru and consisted of six genomic groups comprising two to four isolates and nine unique genotypes. the isolates were most closely related to the two previously genotyped isolates from mexico, with a maximum distance of 2 to 4. the peruvian strains were clearly distinct from the east and west medi ... | 2009 | 19027131 |
| comparative assessment of passive surveillance in disease-free and endemic situation: example of brucella melitensis surveillance in switzerland and in bosnia and herzegovina. | globalization and subsequent growth in international trade in animals and animal products has increased the importance of international disease reporting. efficient and reliable surveillance systems are needed in order to document the disease status of a population at a given time. in this context, passive surveillance plays an important role in early warning systems. however, it is not yet routinely integrated in the assessment of disease surveillance systems because different factors like the ... | 2008 | 19099610 |
| validation of a competitive elisa for diagnosis of brucella melitensis infection in sheep and goats. | a competitive enzyme-linked immunosorbent assay (celisa) was validated for the serodiagnosis of brucella melitensis infection in small ruminants using 2108 positive and 2154 negative reference sera from sheep and goats. the optimum cut-off values, offering the highest diagnostic sensitivity (dsn) and diagnostic specificity (dsp), determined by receiver operating characteristic analysis, were at 23.6%, 21.8% and 25.0% inhibition of the conjugate control for sheep, goats and both species, respecti ... | 2008 | 17574884 |
| virb12 is a serological marker of brucella infection in experimental and natural hosts. | the brucella species type iv secretion system, encoded by the virb1-12 locus, is required for intracellular replication and persistent infection in vivo. the requirement of virb proteins for infection suggests that they are expressed in vivo and may therefore represent serological markers of infection. to test this idea, we purified recombinant virb1, virb5, virb11, and virb12 and tested for their recognition by antibodies in sera from experimentally infected mice and goats by using an indirect ... | 2008 | 18077620 |
| non-imported brucellosis outbreak from unpasteurized raw milk in moroccan immigrants in spain. | nine cases of brucellosis were identified in the city of elche (comunidad valenciana, spain) in two families of moroccan immigrants. all of the patients had drunk unpasteurized raw milk from goats. brucella melitensis biovar 3 was identified in clinical specimens. preventive measures for brucellosis should be implemented among immigrant populations in spain. | 2008 | 18205974 |
| brucellosis in camels (camelus dromedarius) in darfur, western sudan. | in a field outbreak of brucellosis in 21 camels mixed with cattle, sheep and goats, five camels, three of which showed clinical signs, were serologically positive. in a subsequent abattoir survey of apparently healthy camels, six animals were seropositive, albeit with titres that tended to be lower than those found in the field outbreak. of the six seropositive slaughtered camels, five were shown to have lymph nodes (prescapular and supramammary) infected with brucellae (brucella melitensis biov ... | 2008 | 18346482 |
| improved performance of brucella melitensis native hapten over brucella abortus ops tracer on goat antibody detection by the fluorescence polarization assay. | the current method for goat brucellosis diagnosis is based on the world organization for animal health (oie) using the screening card test (ct), with antigen at 8% (ct8) or 3% (ct3) of cell concentrations, and the confirmatory complement fixation test (cft). however, these tests do not differentiate antibodies induced by vaccination from those derived from field infections by brucella species or other bacterial agents; in places like mexico, where the prevalence of brucellosis and the vaccinatio ... | 2008 | 18359093 |
| use of the brucella melitensis native hapten to diagnose brucellosis in goats by a rapid, simple, and specific fluorescence polarization assay. | the performance of the fluorescence polarization assay (fpa) using the recently described brucella melitensis native hapten and the brucella abortus o-polysaccharide tracer was evaluated and compared with those of the world organization for animal health tests related to indirect and competitive enzyme-linked immunosorbent assays as classification variables for goat sera obtained from a high-prevalence area where vaccination was performed; test series were also evaluated to increase the final sp ... | 2008 | 18385457 |
| brucellosis vaccines: assessment of brucella melitensis lipopolysaccharide rough mutants defective in core and o-polysaccharide synthesis and export. | the brucellae are facultative intracellular bacteria that cause brucellosis, one of the major neglected zoonoses. in endemic areas, vaccination is the only effective way to control this disease. brucella melitensis rev 1 is a vaccine effective against the brucellosis of sheep and goat caused by b. melitensis, the commonest source of human infection. however, rev 1 carries a smooth lipopolysaccharide with an o-polysaccharide that elicits antibodies interfering in serodiagnosis, a major problem in ... | 2008 | 18648644 |
| evaluation of brucella melitensis b115 as rough-phenotype vaccine against b. melitensis and b. ovis infections. | brucella melitensis strain rev1 is used as vaccine for the prophylaxis of brucellosis in sheep and goats. because of its smooth phenotype, however, it induces antibodies directed to the o-polysaccharide (o-ps) of the lipopolysaccharide (lps), thus unabling to distinguish between vaccinated and infected animals. it has been speculated that alternative vaccines could be live, attenuated brucella rough strains, which are devoid of the o-ps. b. melitensis b115 is a natural, attenuated, rough strain. ... | 2008 | 18675869 |
| immunohistochemical detection of brucella melitensis antigens in cases of naturally occurring abortions in sheep. | brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. the present study was carried out to determine, by immunohistochemistry, the presence of b. melitensis antigens in 110 naturally occurring aborted sheep fetuses. sections of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect brucella antigens. brucella melitensis antigens were detected in 33 of 110 fetuses (30%). in the 33 positive c ... | 2008 | 18987234 |
| multicenter study of brucellosis in egypt. | brucellosis causes appreciable economic losses in livestock. examination of milk and tissues from animals in egypt for brucella spp. showed increased prevalence rates of serologically reactive animals. all isolates were b. melitensis biovar 3. one brucella sp. was isolated from milk of serologically nonreactive buffaloes. | 2008 | 19046520 |
| investigations on brucellosis in egyptian baladi does with emphasis on evaluation of diagnostic techniques. | investigations were carried out on caprine brucellosis in a costal area in egypt. a total number of 577 baladi does was examined for brucella infection using different serological tests. specimens were taken from seropositive obligatory slaughtered does (no = 33) for brucella isolation, histopathological examination, polymerase chain reaction (pcr) assay and determination of serum copper (cu), zinc(zn) and iron (fe) concentrations. results indicated that the incidence of brucellosis was 3.0-5.0% ... | 2007 | 19070039 |
| validation of a fluorescence polarization assay (fpa) performed in microplates and comparison with other tests used for diagnosing b. melitensis infection in sheep and goats. | fluorescence polarization assay (fpa) is a relatively new test for the serological diagnosis of brucella spp. infection in animals. fpa, carried out in 96-well microplate format, was validated here for diagnosing b. melitensis infection in sheep and goats. this study included sera from 1933 sheep and goats, from animals reared in naturally infected flocks (verified by culture) and showing a positive reaction to two different tests conducted in parallel. in addition, 2154 sera originating from he ... | 2007 | 17258229 |
| application of the fluorescence polarization assay for detection of caprine antibodies to brucella melitensis in areas of high prevalence and widespread vaccination. | the screening rose bengal test (rbt), the buffered plate agglutination test (bpat), and the confirmatory complement fixation test (cft) are currently approved by the world organization for animal health (oie) for diagnosis of goat brucellosis. however, rbt (at 3% or 8% cell concentration) is known to be affected by vaccinal antibodies. in the present study, mexican and canadian oie tests were compared with the fluorescence polarization assay (fpa), alone or in combination, using indirect and com ... | 2007 | 17267588 |
| seroprevalence of and risk factors for brucellosis of goats in herds of michoacan, mexico. | our objectives were to estimate the seroprevalence of brucella melitensis, and to identify some risk factors associated with goat seropositivity in michoacan, mexico. blood samples were collected from 5114 animals from 79 herds. sera were tested for antibodies against b. melitensis using the rose bengal plate test and the complement-fixation test. information regarding the herds and each animal sampled were recorded through a personal interview at the farm. we used random-effects multivariable l ... | 2007 | 17604858 |
| asymptomatic brucella bacteraemia and isolation of brucella melitensis biovar 3 from human breast milk. | brucellosis is a zoonotic disease and virtually all infections derived from exposure to animals or ingestion of unpasteurized dairy products. brucellosis among family members has been reported. however, screening household members of an index case of acute brucellosis is not a routine procedure. a 10-y-old boy was diagnosed with acute brucellosis. unpasteurized goat cheese commonly consumed within the family was thought to be the possible source of the bacteria. the family (parents, sister and b ... | 2007 | 17366048 |
| induction of immune response in mice with a dna vaccine encoding outer membrane protein (omp31) of brucella melitensis 16m. | brucellosis causes serious economic losses to goat farmers by way of reproductive losses in the form of abortions and stillbirths. nucleic acid vaccines provide an exciting approach for antigen presentation to the immune system. in this study, we evaluated the ability of dna vaccine encoding the omp31 protein of brucella melitensis 16m to induce cellular and humoral immune responses in mice. we constructed eukaryotic expression vectors called ptargetomp31, encoding outer membrane protein (omp31) ... | 2007 | 17014873 |
| comparison of fluorescence polarization assay with card and complement fixation tests for the diagnosis of goat brucellosis in a high-prevalence area. | an evaluation of fluorescence polarization assay (fpa) to detect antibodies against brucella melitensis according to the mexican official norm (nom) was performed. in this study, a total of 2582 goat serum samples from a high-prevalence area in northeast mexico where vaccination is applied, were used. of these, 1094 were classified as nom negatives (card test (ct) negatives or ct positives/complement fixation test (cft) negatives) and 1488 as nom positives (ct and cft positives). the receiver op ... | 2006 | 16242780 |
| identification of brucella melitensis 16m genes required for bacterial survival in the caprine host. | brucella species are gram-negative bacteria which belong to alpha-proteobacteria family. these organisms are zoonotic pathogens that induce abortion and sterility in domestic mammals and chronic infections in humans known as malta fever. the virulence of brucella is dependent upon its ability to enter and colonize the cells in which it multiplies. the genetic basis of this aspect is poorly understood. signature-tagged mutagenesis (stm) was used to identify potential brucella virulence factors. p ... | 2006 | 17090391 |
| brucellosis: the first case of king chulalongkorn memorial hospital and review of the literature. | brucellosis remains a major zoonotic disease worldwide. it has never been reported at king chulalongkorn memorial hospital (kcmh). the authors describe the first case of brucellosis in kcmh, and also review all previous reports in thailand. the presented case was a 52-year-old thai man, living in phetchabun province, who was diagnosed with idiopathic pulmonary fibrosis two years prior to admission. he presented with prolonged fever, dry cough, weight loss of eight kg over three months, hepatospl ... | 2006 | 17048447 |
| evaluation of novel brucella melitensis unmarked deletion mutants for safety and efficacy in the goat model of brucellosis. | pregnant goats were employed to assess unmarked deletion mutant vaccine candidates bmdeltaasp24, bmdeltacydba, and bmdeltavirb2, as the target host species naturally infected with brucella melitensis. goats were assessed for the degree of pathology associated with the vaccine strains as well as the protective immunity afforded by each strain against abortion and infection after challenge with wild-type brucella melitensis 16m. both bmdeltaasp24 and bmdeltavirb2 were considered safe vaccine candi ... | 2006 | 16697090 |
| in vitro susceptibilities of brucella melitensis isolates to eleven antibiotics. | brucellosis is an endemic disease present in many countries worldwide, but it is rare in europe and north america. nevertheless brucella is included in the bacteria potentially used for bioterrorism. the aim of this study was the investigation of the antibiotic susceptibility profile of brucella isolates from areas of the eastern mediterranean where it has been endemic. | 2006 | 17014707 |
| development and validation of a competitive elisa kit for the serological diagnosis of ovine, caprine and bovine brucellosis. | a competitive elisa (brucella-ab c-elisa) was standardized and validated for the detection of brucella antibodies in cattle, sheep and goat sera using a monoclonal antibody (mab 4b5a) produced against brucella melitensis biotype 2. the specificity and sensitivity of the assay were 100% to a 67.5% cut-off point (b/bo%). when compared with an indirect elisa, the brucella-ab c-elisa did not demonstrate cross-reactions when testing positive sera for antibodies to some enterobacteriaceae. a compariso ... | 2006 | 17123429 |
| a model of animal-human brucellosis transmission in mongolia. | we developed a dynamic model of livestock-to-human brucellosis transmission in mongolia. the compartmental model considers transmission within sheep and cattle populations and the transmission to humans as additive components. the model was fitted to demographic and seroprevalence data (rose bengal test) from livestock and annually reported new human brucellosis cases in mongolia for 1991-1999 prior to the onset of a mass livestock-vaccination campaign (s19 brucella abortus for cattle and rev 1 ... | 2005 | 15899298 |
| human brucellosis in a nonendemic country: a report from germany, 2002 and 2003. | human brucellosis has become a rare disease in germany since the eradication of bovine and ovine/caprine brucellosis in this country. therefore, most physicians are unfamiliar with the illnesses clinical presentation, diagnostic tools, and therapeutic strategies. this retrospective study was carried out to evaluate the epidemiological, clinical, and laboratory features of human brucellosis in germany in the years 2002 and 2003. thirty-one bacterial isolates from 30 patients sent to the german na ... | 2005 | 15959815 |
| protective properties of rifampin-resistant rough mutants of brucella melitensis. | vaccination against brucella infections in animals is usually performed by administration of live attenuated smooth b. abortus strain s19 and b. melitensis strain rev1. they are proven effective vaccines against b. abortus in cattle and against b. melitensis and b. ovis in sheep and goats, respectively. however, both vaccines have the main drawback of inducing o-polysaccharide-specific antibodies that interfere with serologic diagnosis of disease. in addition, they retain residual virulence, bei ... | 2005 | 15972510 |
| [occupational brucellosis in slaughtering of sheep and goats: study of five cases from a municipal abattoir in south-eastern sicily]. | brucellosis is a world-wild zoonosis of bacterial origin. in italy, where reporting of the disease is mandatory, the incidence is 2.1 cases every 100,000 inhabitants, but tends to be higher in southern regions. however, the incidence decreased from 1999 to 2002. | 2005 | 16001513 |
| brucellosis. | 2005 | 16156056 | |
| reliability evaluation of sampling plan fixed by council directive 91/68/eec for the maintenance of officially brucellosis-free flock status. | the european union (eu) strategy with respect to sheep and goat brucellosis aims to eradicate the infection and achieve officially brucellosis-free (obf) status in all eu holdings and territories. council directive 91/68/eec of 28 january 1991 states that to maintain obf status of ovine or caprine holdings located outside an obf territory, only a representative number of animals need to be tested annually. however, depending on the number of animals in a holding, this testing method risks non-de ... | 2005 | 16219092 |
| a case of human brucellosis in hong kong. | brucellosis is an infectious disease of humans and animals caused by brucella species. we report on a 34-year-old housewife who presented with recurrent headache, fever, and malaise. blood cultures yielded slow-growing gram-negative coccobacilli that were later identified as brucella melitensis. the patient recalled handling goat placenta in china. she was prescribed a 6-week course of doxycycline and rifampicin. laboratory staff who had been exposed to the isolate remained asymptomatic. the epi ... | 2005 | 16219962 |
| validation of fluorescence polarization assay (fpa) and comparison with other tests used for diagnosis of b. melitensis infection in sheep. | fluorescence polarization assay (fpa) is a new test for the serological diagnosis of brucella spp. infection in animals. the fpa is validated for the diagnosis of b. melitensis infection in sheep. for this purpose, 166 sera originated from natural infected sheep (verified by culture) and 851 sera originated from healthy animals (reared in areas where b. melitensis was never been isolated) were tested. the optimum cut-off value that offers the highest diagnostic sensitivity (dsn) and diagnostic s ... | 2005 | 16278056 |
| whole-genome analyses of speciation events in pathogenic brucellae. | despite their high dna identity and a proposal to group classical brucella species as biovars of brucella melitensis, the commonly recognized brucella species can be distinguished by distinct biochemical and fatty acid characters, as well as by a marked host range (e.g., brucella suis for swine, b. melitensis for sheep and goats, and brucella abortus for cattle). here we present the genome of b. abortus 2308, the virulent prototype biovar 1 strain, and its comparison to the two other human patho ... | 2005 | 16299333 |
| [brucellosis at the dawn of the 21st century]. | human brucellosis is now a rare disease in countries where eradication programs (especially vaccination) against brucellosis in cattle, sheep, and goats have been successfully implemented. in france, fewer than 50 brucellosis cases are annually notified to the national institute for infection surveillance. human brucellosis, however, remains endemic in the mediterranean basin, middle east, western asia, africa, and south america. shortcomings of standard diagnostic methods for brucellosis (varia ... | 2005 | 15695027 |
| indirect enzyme-linked immunosorbent assay for detection of brucella melitensis-specific antibodies in goat milk. | brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. few cases of b. melitensis infection in goats have occurred in the united states over the last 25 years. however, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not introduced into the u.s. goat population. the objective of this study was to develop a sensitive and specific indirect enzyme-linked immunosorbent assay (ielisa) for the detection of ... | 2005 | 15695670 |
| from the discovery of the malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. | brucellosis is not a sustainable disease in humans. the source of human infection always resides in domestic or wild animal reservoirs. the routes of infection are multiple: food-borne, occupational or recreational, linked to travel and even to bioterrorism. new brucella strains or species may emerge and existing brucella species adapt to changing social, cultural, travel and agricultural environment. brucella melitensis is the most important zoonotic agent, followed by brucella abortus and bruc ... | 2005 | 15845228 |
| human and animal brucellosis in jordan between 1996 and 1998: a study. | between 1996 and 1998, a total of 2,494 samples of blood from humans and animals were collected and tested for brucellosis. this total included 1,594 samples of animal blood, collected from 1,050 sheep from 20 flocks, and 544 goats from eight herds. the serum samples were tested using the rose bengal test, the tube agglutination test, the complement fixation test and an enzyme-linked immunosorbent assay. moreover, a complete history was compiled from each flock/herd. the rate of abortions in she ... | 2004 | 15861878 |
| comparison of serological tests for the detection of ovine and caprine antibody to brucella melitensis. | the indirect enzyme-linked immunosorbent assay (ielisa), the competitive enzyme-linked immunosorbent assay (celisa) and the fluorescence polarisation assay (fpa) were evaluated with sera from sheep experimentally infected with brucella melitensis and negative canadian sheep. the sensitivity and specificity of the assays were as follows: ielisa: 91.7% and 97.6%, celisa: 75.0% and 99.8% and fpa: 91.7% and 89.5%. sera from the same experimental population were divided according to serological react ... | 2004 | 15861894 |
| protection against brucellosis in goats, five years after vaccination with reduced-dose brucella melitensis rev 1 vaccine. | the protection conferred by the reduced-dose rev 1 brucella melitensis vaccine in goats that had been immunized 5 years previously was evaluated. sixteen goats vaccinated 5 years before with rev 1 (1 x 10(5) cfu) and 5 non-vaccinated goats were challenged with b. melitensis 16m (4 x 10(5) cfu) using the conjunctival route. after giving birth or aborting, the goats were sacrificed and tissue samples were taken for bacteriological study. the challenge strain was recovered in 12%, of the animals fr ... | 2004 | 14998310 |
| survey of the seroprevalence of brucellosis in ruminants in kosovo. | a cross-sectional survey of the seroprevalence of brucellosis in sheep, goats and cattle in kosovo was made in january 2001. a total of 12,000 serum samples, from 7941 cattle, 3548 sheep and 511 goats, were screened using the rose bengal test. doubtful and positive results were further tested with competitive and indirect elisas. the overall serological prevalences derived from the samples positive to all three tests, were 6.26 per cent (95 per cent confidence intervals [ci] 5.5 to 7.1 per cent) ... | 2004 | 15224594 |
| role of catalase in the virulence of brucella melitensis in pregnant goats. | an isogenic kate mutant derived from virulent brucella melitensis 16m displays hypersensitivity to hydrogen peroxide in disk sensitivity assays but retains the capacity to colonize pregnant goats and induce abortion. these experimental findings indicate that although the sole periplasmic catalase of brucella melitensis functions as an antioxidant, this enzyme does not play a critical role in virulence in the natural host. | 2004 | 15288933 |
| childhood brucellosis--a microbiological, epidemiological and clinical study. | a total of 5726 blood specimens (from children aged 14 years and younger) were studied for the serological evidence of brucellosis. ninety-three (1.6 per cent) showed diagnostic agglutinin titres with a geometric mean titre of 403 (sd +/- 547). forty-three (59.7 per cent) blood specimens yielded the growth of brucella melitensis. thirty-nine patients (41.93 per cent) were shepherds, who constituted the major occupational group affected in the present series. more than 60 per cent of the patients ... | 2004 | 15233191 |
| use of an immunobinding test on nitrocellulose paper to diagnose caprine brucellosis. | 2004 | 14989360 | |
| molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans. | brucellosis caused by brucella spp. is a major zoonotic disease. control of brucellosis in agricultural animals is a prerequisite for the prevention of this disease in human beings. recently, brucella melitensis was declared by the centers for disease control and prevention to be one of three major bioterrorist agents due to the expense required for the treatment of human brucellosis patients. also, the economic agricultural loss due to bovine brucellosis emphasizes the financial impact of bruce ... | 2003 | 12525425 |
| [evaluation of 2 hemoculture media for the isolation of brucella spp.]]. | the diagnostic efficiency of two hemoculture media for the detection of different species of brucella strains was evaluated. strains of brucella melitensis, brucella suis, brucella abortus, brucella ovis, and brucella abortus s19 were used. each strain was diluted in phosphate buffer saline (pbs) to obtain a concentration of 10(5) colony forming units/ml (cfu/ml). blood from goats, pigs, cattle, and sheep was mixed with the bacterial suspension to obtain a final concentration minor or equal to 1 ... | 2003 | 14587372 |
| brucellosis outbreak due to unpasteurized raw goat cheese in andalucia (spain), january - march 2002. | eleven brucellosis cases were identified in three municipalities of cordoba (andalucia, spain). a case-control study was conducted, selecting three cases per control. persons having eaten unpasteurized raw goat cheese produced in a farmhouse located in the epidemic territory, were at higher risk for presenting brucellosis (or=21.6, ic95%=1.6-639.8). brucella melitensis serovar 3 was identified in clinical specimens and in goat tissue and milk samples from the herd's farmhouse. preventive measure ... | 2003 | 12941982 |
| the genome sequence of the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and malta fever in humans. the genome of b. melitensis strain 16m was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 orfs. by using the bioinformatics suite ergo, 2,487 (78%) orfs were assigned functions. the origins of replication of the two chromosomes are similar to those of other alpha-proteobacter ... | 2002 | 11756688 |
| identification of the brucella melitensis vaccine strain rev.1 in animals and humans in israel by pcr analysis of the psti site polymorphism of its omp2 gene. | adverse effects of strain persistence and secretion in milk have been encountered with the brucella melitensis vaccine strain rev.1. field isolates obtained from vaccinated animals and from a human resembled the vaccine strain rev.1 by conventional bacteriological tests. the lack of a specific molecular marker that could specifically characterize the commercial vaccine strain prevented confirmation of the homology of the rev.1-like field isolates to the vaccine strain. the composition of the omp ... | 2002 | 11923376 |
| identification of a quorum-sensing signal molecule in the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a gram-negative alpha2-proteobacterium responsible for abortion in goats and for malta fever in humans. this facultative intracellular pathogen invades and survives within both professional and nonprofessional phagocytes. a dichloromethane extract of spent culture supernatant from b. melitensis induces bioluminescence in an escherichia coli acyl-homoserine lactone (acyl-hsl) biosensor strain based upon the activity of the lasr protein of pseudomonas aeruginosa. hplc fracti ... | 2002 | 12010991 |
| identification of brucella melitensis vaccine strain rev.1 by pcr-rflp based on a mutation in the rpsl gene. | the live attenuated strain b. melitensis rev.1 is considered the best vaccine available for the prophylaxis of brucellosis in sheep and goats. the rev.1 vaccine was obtained in the 1950s by a two-step selection involving firstly streptomycin resistance and dependence and secondly reversion of dependence but keeping streptomycin resistance. chromosomally acquired streptomycin resistance is frequently due to mutations in the gene encoding the ribosomal protein s12, rpsl. nucleotide sequencing reve ... | 2002 | 12057611 |
| pathogenicity and protective activity in pregnant goats of a brucella melitensis deltaomp25 deletion mutant. | the brucella melitensis mutant bm 25, which lacks the major 25 kda outer membrane protein omp25, has previously been found to be attenuated in the murine brucellosis model. in the present study, the capacity of the deltaomp25 mutant to colonise and cause abortions in the caprine host was evaluated. the vaccine potential of bm 25 was also investigated in goats. inoculation of nine pregnant goats in late gestation with the b. melitensis mutant resulted in 0/9 abortions, while the virulent parental ... | 2002 | 12076120 |
| global analysis of the brucella melitensis proteome: identification of proteins expressed in laboratory-grown culture. | brucella melitensis is a facultative intracellular bacterial pathogen that causes brucellosis, a zoonotic disease primarily infecting sheep and goats, characterized by undulant fever, arthritic pain and other neurological disorders in humans. a comprehensive proteomic study of strain 16m was conducted to identify and characterize the proteins expressed in laboratory-grown culture. using overlapping narrow range immobilized ph gradient strips for two-dimensional gel electrophoresis, 883 protein s ... | 2002 | 12203900 |
| an overview of the eradication of brucella melitensis from kwazulu-natal. | brucella melitensis is a gram-negative bacterium whose primary hosts are goats and sheep. like the other brucelia spp., with the exception of brucella ovis, it is not particularly host specific as it is pathogenic for a variety of other mammal species including humans. in humans the disease caused by it is rated as one of the most important zoonoses. three outbreaks have been recorded in goats and sheep in south africa; the first outbreak occurred in sheep in 1965 in the mpumalanga and northern ... | 2002 | 12233997 |
| incidence and control of brucellosis in the near east region. | in countries of the near east region, brucellosis was reported in almost all domestic animals, particularly cattle, sheep and goats. brucellosis in camels has been reported in saudi arabia, kuwait, oman, iraq, iran, sudan, egypt, libya and somalia. it has been reported even in racing camels in the united arab emirates. in egypt, brucellosis has been reported also in buffaloes, equines and swine. brucella melitensis biovar 3 is the most commonly isolated species from animals in egypt, jordan, isr ... | 2002 | 12414137 |
| brucellosis in the european union and norway at the turn of the twenty-first century. | control and eradication programs of brucellosis in cattle, sheep, goats and pigs have been more or less successfully implemented within the member states (ms) of the european union (eu) and norway after word war ii. as a result, the epidemiological situation of animal brucellosis is extremely diverse among different ms or regions within a ms and among the different animal species. some ms, mainly north european countries, and norway are declared "officially bovine brucellosis free" and/or "offic ... | 2002 | 12414139 |
| an overview of the epidemiology and epizootology of brucellosis in selected countries of central and southeast europe. | the objective of this paper is to give an overview of the epidemiologic and epizootic status of brucellosis in selected countries of central and southeast europe (balkan region). based on dimension of the disease problem, there is a need to establish collaboration in the eradication and prevention of brucellosis between all countries in the region. although there were no readily accessible data concerning epidemiology and epizootology of brucellosis in these countries, the limited official and p ... | 2002 | 12414140 |
| an epidemiological and surveillance overview of brucellosis in romania. | this article reports epidemiological investigations on the occurrence of brucellosis in romania. like in other former communist countries, data concerning epidemiology of brucellosis and published articles are very few. the epidemiology and control of brucellosis in romania was analyzed using data made available by the office international des epizooties and veterinary service of romania. romania, like many other developed countries, has eradicated brucella abortus from cattle since 1969. brucel ... | 2002 | 12414141 |
| epidemiology, zoonotic aspects, vaccination and control/eradication of brucellosis in india. | in india, brucellosis was first recognised in 1942 and is now endemic throughout the country. the disease is reported in cattle, buffalo, sheep, goats, pigs, dogs and humans. b. abortus biotype-1 in cattle and buffaloes and b. melitensis biotype-1 in sheep, goats and man are the predominant infective biotypes. the long-term serological studies have indicated that 5% of cattle and 3% of buffaloes are infected with brucellosis. economic losses due to brucellosis in livestock are considerable in an ... | 2002 | 12414143 |
| characterization of the caprine model for ruminant brucellosis. | the relationship between man, the goat, and brucellosis is historical. today brucella melitensis and brucella abortus pose a serious economic and public health threat in many countries throughout the world. infection of pregnant goats and sheep with b. melitensis results in abortion during the third trimester of pregnancy. although nearly eradicated in the us, bovine brucellosis is still a problem in many countries and the potential for re-infection of domestic stock from wildlife reservoirs in ... | 2002 | 12414161 |
| control of small ruminant brucellosis by use of brucella melitensis rev.1 vaccine: laboratory aspects and field observations. | brucellosis vaccines are essential elements in control programs. since first developed in the mid-1950s, the brucella melitensis vaccine strain rev.1 has been used worldwide and its significant value in protecting sheep and goats in endemic areas recognized. this review provides historical background on the development of the vaccine, its use and field complications arising in israel following changes in the strain's pathogenicity. the urgent need for resolving cases of vaccine strain excretion ... | 2002 | 12414167 |
| risk-based design of repeated surveys for the documentation of freedom from non-highly contagious diseases. | the documentation of freedom from disease requires reliable information on the actual disease status in a specific animal population. the implementation of active surveillance (surveys) is an effective method to gain this information. for economical reasons, the sample size should be as small as possible but large enough to achieve the required confidence level for a targeted threshold. when conducting surveys repeatedly, various information sources about the disease status of the population can ... | 2002 | 12441234 |
| bacteria-modified amperometric immunosensor for a brucella melitensis antibody assay. | a novel amperometric immunosensor setup is described which uses horseradish peroxidase (hrp) as a label in conjunction with a current-based brucella sensor. the bacteria modified immunosensor was constructed by using a biocomposite formed by dispersing graphite powder into a mixture of brucella melitensis and silicate polymer gel. the enzyme-labeled antibody can readily diffuse toward the encapsulated antigen (brucella melitensis), which retains its binding properties, and the association reacti ... | 2002 | 12083544 |
| spinal brucellosis: case report in the united states. | case report. | 2002 | 11979184 |
| acute cholecystitis caused by brucella melitensis: case report and review. | brucellosis is a common zoonosis in many parts of the world. human brucellosis is a multisystemic disease with a wide range of clinical symptoms. biliary involvement in the form of acute cholecystitis is a very rare manifestation of brucellosis. here we report the case of a 34-year-old male with acute cholecystitis caused by brucella melitensis and review seven other cases. we conclude that brucella may have a focal effect on the gallbladder, in the form of acute cholecystitis. | 2001 | 11243761 |
| [bacteremia caused by brucella sp. with negative conventional serology]. | 2001 | 11256247 | |
| re-examination of the role of the brucella melitensis htra stress response protease in virulence in pregnant goats. | based on previously reported studies describing the experimental infection of pregnant goats with b. melitensis strain rwp5, we proposed that the htra protease plays an important role in the virulence of b. melitensis in its natural ruminant host. subsequent studies, however, have shown that rwp5 is actually an htra cycl double mutant. in order to definitively evaluate the role of the b. melitensis htra in virulence, we constructed an authentic htra mutant and examined this strain in pregnant go ... | 2001 | 11423199 |
| use of recombinant bp26 protein in serological diagnosis of brucella melitensis infection in sheep. | previously a brucella protein named cp28, bp26, or omp28 has been identified as an immunodominant antigen in infected cattle, sheep, goats, and humans. in the present study we evaluated antibody responses of infected and b. melitensis rev.1-vaccinated sheep to the bp26 protein using purified recombinant bp26 protein produced in escherichia coli in an indirect enzyme-linked immunosorbent assay (i-elisa). the specificity of the i-elisa determined with sera from healthy sheep (n = 106) was 93%. the ... | 2001 | 11427425 |
| brucellosis control in saudi arabia: prospects and challenges. | brucellosis is a zoonotic disease of worldwide distribution. despite its control in many developed countries the disease remains endemic in saudi arabia where the national seroprevalence of the disease is 15%. in saudi arabia the disease is introduced through uncontrolled importation of animals that are poorly screened for the disease. every year the kingdom imports a few million heads of sheep and goats for sacrifice during hajj from africa, india, and autstralia. brucella melitensis remains th ... | 2001 | 11434523 |
| brucellosis among animals and human contacts in eastern sudan. | to study the relative frequency of brucellosis among domestic animals in kassala state, sudan, in the year 1999 and compare the results of previous studies since 1908. also to study the frequency rates of the disease in animal contacts in the area. | 2001 | 11479636 |
| risk factors for brucella seropositivity in goat herds in eastern and western uganda. | cross-sectional prevalences and risk factors for brucella seropositivity in goats in eastern and western uganda were investigated. serum was collected from 1518 goats randomly selected from 145 herds which had been identified using multistage sampling. the brucellosis card test (ct) and the brucella melitensis tube-agglutination test (tat) were used in parallel to detect antibodies against b. abortus and b. melitensis, respectively. interviewer-administered questionnaires were used to collect in ... | 2001 | 11679168 |
| field investigations of brucellosis in cattle and small ruminants in syria, 1990-1996. | the authors present the epidemiological status of brucellosis in cattle and small ruminants in syria from 1990 to 1996, based on laboratory findings at the brucellosis centre, damascus. initial investigations using the rose bengal plate test, the complement fixation test and a miniaturised variant of the slow agglutination test were conducted throughout the country in 1990 and 1991, revealing an overall herd seroprevalence rate of 3.14% in cattle herds and 2.94% in small ruminant flocks. althoug ... | 2001 | 11732419 |
| assessment of a monoclonal antibody-based competitive enzyme linked immunosorbent assay (celisa) for diagnosis of brucellosis in infected and rev. 1 vaccinated sheep and goats. | in this study a celisa for the diagnosis of brucellosis due to b. melitensis in sheep and goats was evaluated and its capability of discriminating vaccinated from infected animals was assessed. information is provided indicating that the celisa has a diagnostic sensitivity (99.4%) and specificity (98.9%) in sheep and goats comparable to that of many standard indirect elisa methods. in addition, the test proved able to distinguish between vaccinated and infected animals with an accuracy of up to ... | 2000 | 11061628 |
| identification and characterization of in vivo attenuated mutants of brucella melitensis. | brucella melitensis 16m is a gram-negative alpha2-proteobacterium responsible for abortion in goats and for malta fever in humans. this facultative intracellular pathogen invades into and survives within both professional and non-professional phagocytes. signature-tagged mutagenesis (stm) was used to identify genes required for the in vivo pathogenesis of brucella. a library of transposon mutants was screened in a murine infection model. out of 672 mutants screened, 20 were not recovered after a ... | 2000 | 11069678 |
| [meningitis as the first and only manifestation of brucellosis]. | an 8-year-old girl with no previous symptoms was admitted to hospital with aseptic meningitis syndrome. the girls father is a shepherd and there was a familial antecedent of brucellosis, but the patient did not consume unpasteurized goat milk products nor did she take part in the care of the sheep stock. the clinical evolution did not follow the usual development of a viral or partially treated bacterial meningitis. brucella melitensis was isolated in the cerebrospinal fluid and blood. specific ... | 2000 | 11083975 |
| molecular characterization of brucella strains isolated from marine mammals. | recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as brucella species by conventional phenotypic analysis. this study found the 16s rrna gene from one representative isolate was identical to the homologous sequences of brucella abortus, b. melitensis, b. canis, and b. suis. is711-based dna fingerprinting of 23 isolates from marine mammals showed all the isolates differed from the classical brucella species. in general, fingerprint patterns grouped by ... | 2000 | 10699036 |
| brucella melitensis infection discovered in cattle for first time, goats also infected. | 2000 | 10707672 | |
| [incidence, etiology and epidemiology of brucellosis in a rural area of the province of lleida]. | this a prospective study of the incidence, etiology and epidemiological profile of human brucellosis in the regions of pallars jussà y sobirà (lleida) for the 1995-1998 period. | 2000 | 10832390 |