Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| survival of escherichia coli o157:h7, listeria monocytogenes 4b and yersinia enterocolitica o3 in different yogurt and kefir combinations as prefermentation contaminant. | to compare microbiological safety of yogurt, kefir and different combinations of yogurt and kefir samples by using three foodborne pathogenic strains (escherichia coli o157:h7, listeria monocytogenes 4b and yersinia enterocolitica o3) as indicators. | 2003 | 12911712 |
| listeriosis. | 2003 | 12913780 | |
| influence of marinades on survival during storage of acid-adapted and nonadapted listeria monocytogenes inoculated post-drying on beef jerky. | the objective of the present study was to investigate the survival of acid-adapted and nonadapted listeria monocytogenes inoculated post-drying on dried beef slices (beef jerky), which were treated (24 h, 4 degrees c) with the following marinades before drying at 60 degrees c for 10 h: (1) control (c), (2) traditional marinade (tm), (3) modified marinade; double the amount of tm with added 1.2% sodium lactate, 9% acetic acid, and 68% soy sauce with 5% ethanol (mm), (4) dipping into 5% acetic aci ... | 2003 | 12915039 |
| listeria spp. contamination in piggeries: comparison of three sites of environmental swabbing for detection and risk factor hypothesis. | listeria monocytogenes is a foodborne pathogen of major concern for public health in industrialized countries. listeria carriage by pigs at the herd level could be a primary source for carcass contamination. forty-seven finishing pig facilities were involved in the present study designed to compare three environmental swabbing sites in order to detect listeria spp. in piggeries. swabs were taken from the pen walls, the perianal regions of the pigs and the trough/feeder of the piggery premises. l ... | 2003 | 12916687 |
| interactions of the bacteriocins sakacin p and nisin with food constituents. | bacteriocins are amphiphilic peptides susceptible to adsorption to food macromolecules and proteolytic degradation. these properties may limit their use as preservation agents. the aim of the present work has been to elucidate the fate of the bacteriocin sakacin p in food. nisin was used in a few experiments for comparison. recovery of bacteriocins was studied in homogenates of cold-smoked salmon, chicken cold cuts and raw chicken, with verification of the results in the corresponding food produ ... | 2003 | 12927705 |
| the influence of process parameters for the inactivation of listeria monocytogenes by pulsed electric fields. | the influence of the electric field strength, the treatment time, the total specific energy and the conductivity of the treatment medium on the listeria monocytogenes inactivation by pulsed electric fields (pef) has been investigated. l. monocytogenes inactivation increased with the field strength, treatment time and specific energy. a maximum inactivation of 4.77 log(10) cycles was observed after a treatment of 28 kv/cm, 2000 micros and 3490 kj/kg. the lethal effect of pef treatments on l. mono ... | 2003 | 12927710 |
| bacteriocin-producing enterococcus casseliflavus im 416k1, a natural antagonist for control of listeria monocytogenes in italian sausages ("cacciatore"). | the bacteriocinogenic enterococcus casseliflavus im 416k1 (bac+) isolated from italian sausages or its bacteriocin enterocin 416k1, with strong anti-listerial activity, were used in trials to evaluate the effect on listeria monocytogenes nctc 10888 in artificially inoculated italian sausages ("cacciatore"). in trials with enterocin 416k1 added, l. monocytogenes showed a significant reduction as compared to the control inoculated with l. monocytogenes alone. the elimination of l. monocytogenes wa ... | 2003 | 12927720 |
| stability of electrolyzed oxidizing water and its efficacy against cell suspensions of salmonella typhimurium and listeria monocytogenes. | electrolyzed oxidizing (eo) water has proved to be effective against foodborne pathogens attached to cutting boards and poultry surfaces and against spoilage organisms on vegetables; however, its levels of effectiveness against listeria monocytogenes and salmonella typhimurium in cell suspensions have not been compared with those of other treatments. in this study, the oxidation reduction potentials (orps), chlorine concentrations, and phs of acidic and basic eo water were monitored for 3 days a ... | 2003 | 12929823 |
| selection of fluorescent probes for flow cytometric viability assessment of listeria monocytogenes exposed to membrane-active and oxidizing disinfectants. | the aim of this study was to select fluorescence methods for use as alternatives to plate counting to assess the viability of listeria monocytogenes cells exposed to benzalkonium chloride (bac) and hydrogen peroxide, two disinfectants with different mechanisms of action. a further aim of this study was to determine whether growth phase influences fluorescence labeling and whether it is possible to predict whether a probe will be a good viability indicator for cells exposed to a certain disinfect ... | 2003 | 12929825 |
| prevalence of listeria monocytogenes subtypes in bulk milk of the pacific northwest. | the prevalence of listeria monocytogenes in bulk milk from three pacific northwest states was assessed for 474 herds at three time points. for samples collected in november 2000 and june 2001, the l. monocytogenes prevalence levels were 4.9 and 7.0%, respectively. all isolates were subtyped by serotyping and by pulsed-field gel electrophoresis (pfge). forty-nine of the 55 isolates belonged to serogroup 1/2a, while 6 belonged to serogroup 4. subtyping by pfge revealed that isolates from 31 herds ... | 2003 | 12929828 |
| inhibition of listeria monocytogenes in cold-smoked salmon by carnobacterium piscicola cs526 isolated from frozen surimi. | strain cs526 was isolated from frozen surimi and identified as a bacteriocin producer that had strong inhibitory activity against listeria monocytogenes. strain cs526 was identified as carnobacterium piscicola by partial 16s rdna sequence similarity. the ability of this bacteriocinogenic strain and nonbacteriocinogenic c. piscicola jcm5348 to inhibit the growth of l. monocytogenes was examined in culture broth incubated at 12 degrees c and cold-smoked salmon stored at 4, 12, and 20 degrees c. l. ... | 2003 | 12929829 |
| use of pulsed-field gel electrophoresis to monitor a five-strain mixture of listeria monocytogenes in frankfurter packages. | in a previous study, the viability of a five-strain mixture of listeria monocytogenes (including scott a [serotype 4b, clinical isolate], 101m [serotype 4b, beef-pork sausage isolate], f6854 [serotype 1/2a, turkey frankfurter isolate], h7776 [serotype 4b, frankfurter isolate], and mfs-2 [serotype 1/2a, pork plant isolate]) was monitored during refrigerated storage of frankfurters prepared with and without 3.0% added potassium lactate. throughout a 90-day period of storage at 4 degrees c, the ini ... | 2003 | 12929838 |
| effects of ph and agitation on the growth of listeria monocytogenes scott a in brain heart infusion broth containing combined potassium lactate and sodium diacetate during storage at 4 or 10 degrees c. | the objective of this study was to compare the effects of ph on the growth kinetics of listeria monocytogenes scott a in static and agitated broths stored at 4 and 10 degrees c with and without a combination of 1.85% potassium lactate (pl) and 0.13% sodium diacetate (sda) (3.3% of a 60% commercial solution, purasal p opti. form 4). the ph of brain heart infusion broth without (control) or with 1.85% pl + 0.13% sda was adjusted to 5.5, 6.0, 6.5, and 7.5. l. monocytogenes scott a was inoculated (a ... | 2003 | 12929839 |
| spread of bacterial pathogens during preparation of freshly squeezed orange juice. | to study the potential of three bacterial pathogens to cross-contaminate orange juice during extraction, normal operation conditions during juice preparation at food service establishments were simulated. the spread of salmonella enterica serovar typhimurium, escherichia coli o157:h7, and listeria monocytogenes from inoculated oranges to work surfaces and to the final product was determined. the transference of these three bacterial pathogens to orange juice made from uninoculated oranges with t ... | 2003 | 12929844 |
| an enterococcus faecium secreted antigen, saga, exhibits broad-spectrum binding to extracellular matrix proteins and appears essential for e. faecium growth. | a gene encoding a major secreted antigen, saga, was identified in enterococcus faecium by screening an e. faecium genomic expression library with sera from patients with e. faecium-associated endocarditis. recombinant saga protein showed broad-spectrum binding to extracellular matrix (ecm) proteins, including fibrinogen, collagen type i, collagen type iv, fibronectin, and laminin. a fibrinogen-binding protein, purified from culture supernatants of an e. faecium clinical isolate, was found to mat ... | 2003 | 12933846 |
| influence of pregnancy on the pathogenesis of listeriosis in mice inoculated intragastrically. | pregnancy increases the risk of listeriosis, a systemic disease caused by listeria monocytogenes. however, there is incomplete agreement on the reasons for this increased risk. we examined two features of listeriosis in gravid and nongravid female mice following intragastric (gavage) inoculation, namely, (i) disease severity (measured by lethality) and (ii) listerial infectivity (measured by liver and spleen colonization levels up to 120 h postinoculation). two listerial strains of differing ser ... | 2003 | 12933865 |
| [meningitis caused by listeria monocytogenes in the patient with multiple myeloma]. | 2003 | 12934487 | |
| cardiac tamponade in patients with human immunodeficiency virus disease. | the characteristics of cardiac tamponade in patients with human immunodeficiency virus (hiv) disease were examined by evaluating the cases, case series, and related articles, including autopsy series, identified through a comprehensive literature search. one-hundred eighty-five cases of cardiac tamponade have been reported in patients with hiv disease. sex data were available in 176 patients, of whom 154 (87%) were males. the mean age was 34.7 +/- 10.4 years (range, 11 months to 61 years). mean ... | 2003 | 12934767 |
| meningoencephalitis in a previously healthy nine-year-old girl. | 2003 | 12938682 | |
| the ctsr regulator of listeria monocytogenes contains a variant glycine repeat region that affects piezotolerance, stress resistance, motility and virulence. | a spontaneous high hydrostatic pressure (hhp)-tolerant mutant of listeria monocytogenes scotta, named ak01, was isolated previously. this mutant was immotile and showed increased resistance to heat, acid and h2o2 compared with the wild type (wt) (karatzas, k.a.g. and bennik, m.h.j. 2002 appl environ microbiol 68: 3183-3189). in this study, we conclusively linked the increased hhp and stress tolerance of strain ak01 to a single codon deletion in ctsr (class three stress gene repressor) in a regio ... | 2003 | 12940983 |
| ena/vasp proteins contribute to listeria monocytogenes pathogenesis by controlling temporal and spatial persistence of bacterial actin-based motility. | the listeria monocytogenes surface protein acta mediates actin-based motility by interacting with a number of host cytoskeletal components, including ena/vasp family proteins, which in turn interact with actin and the actin-binding protein profilin. we employed a bidirectional genetic approach to study ena/vasp's contribution to l. monocytogenes movement and pathogenesis. we generated an acta allelic series within the defined ena/vasp-binding sites and introduced the resulting mutant l. monocyto ... | 2003 | 12940993 |
| d and z-values for listeria monocytogenes and salmonella typhimurium in packaged low-fat ready-to-eat turkey bologna subjected to a surface pasteurization treatment. | the d-values of listeria monocytogenes and salmonella typhimurium at various surface pasteurization temperatures were determined for low-fat turkey bologna. four cm2 meat squares were sterilized by irradiation prior to inoculation with 0.1 ml of a 10(8) cfu/ml culture, aseptically packaged in a linear low-density polyethylene pouch, and vacuum-sealed. thermal treatments were administered by submerging packages in a heated water bath maintained at various set temperatures. at an 85 degrees c wate ... | 2003 | 12943307 |
| thalidomide modulation of the immune response in female b6c3f1 mice: a host resistance study. | previously, we have reported that thalidomide (thd) treatment can modulate the immune responses in female b6c3f1 mice. the present study was designed to evaluate whether or not these immunomodulatory responses were of sufficient magnitude to alter host resistances in a number of pathogen and tumor models. b6c3f1 mice were treated intraperitoneally with thd (30-150 mg/kg) for 14 or 28 days, then inoculated with either plasmodium yeolii, pyb6 fibrosarcoma tumor cells, b16f10 melanoma tumor cells, ... | 2003 | 12946441 |
| real-time application of automated ribotyping and dna macrorestriction analysis in the setting of a listeriosis outbreak. | a cluster of three cases of listeriosis cases occurred against a background of endemic listeriosis in western australia. human and environmental isolates of listeria monocytogenes obtained during the outbreak investigation were rapidly subtyped by automated ribotyping using an ecori protocol and a riboprinter. dna macrorestriction analysis by pulsed-field gel electrophoresis (pfge) was used to confirm the relatedness of isolates. serogroup 1/2 predominated among the food samples and the four cli ... | 2003 | 12948362 |
| genome diversification in phylogenetic lineages i and ii of listeria monocytogenes: identification of segments unique to lineage ii populations. | thirteen different serotypes of listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. although the known virulence genes are present in all serotypes, greater than 90% of human cases of listeriosis are caused by serotypes 1/2a, 1/2b, and 4b and nearly all outbreaks of food-borne listeriosis have been caused by serotype 4b strains. phylogenetic analysis of these three common clinical serotypes places them into two different lineages, with serotyp ... | 2003 | 12949110 |
| modulation of hepatic cytochrome p450 during listeria monocytogenes infection of the brain. | hepatic cytochrome p450 enzymes can be modulated during systemic infections. inflammatory responses in the brain have also been shown to cause a significant decrease in the levels and activities of important cytochrome p450 isoforms in the liver. we determined some of the effects of central nervous system (cns) listeria monocytogenes infection on hepatic cytochrome p450 systems in rats. intracerebroventricular injection of l. monocytogenes resulted in a time-dependent modulation of cyp1a, cyp2b, ... | 2003 | 12950004 |
| genes controlled by the essential yycg/yycf two-component system of bacillus subtilis revealed through a novel hybrid regulator approach. | the yycg/yycf two-component system, originally identified in bacillus subtilis, is very highly conserved and appears to be specific to low g + c gram-positive bacteria. this system is required for cell viability, although the basis for this and the nature of the yycf regulon remained elusive. using a combined hybrid regulator/transcriptome approach involving the inducible expression of a phop'-'yycf chimerical protein in b. subtilis, we have shown that expression of yoch, which encodes a potenti ... | 2003 | 12950927 |
| sustained response initiation is required for t cell clonal expansion but not for effector or memory development in vivo. | the factors determining whether an immune response is productive are poorly understood. to understand the circumstances affecting the early stage of the immune response which determine whether memory is generated, the cd8 t cell response was mapped in detail following immunization with live or heat-killed bacteria. our results demonstrate that even in response to a weak immunogen, functional memory cell development is linked to effector cell induction in lymphoid and nonlymphoid tissues. the mai ... | 2003 | 12960304 |
| bacterial pathogens modulate an apoptosis differentiation program in human neutrophils. | human polymorphonuclear leukocytes (pmns or neutrophils) are essential to the innate immune response against bacterial pathogens. recent evidence suggests that pmn apoptosis facilitates resolution of inflammation during bacterial infection. although progress has been made toward understanding apoptosis in neutrophils, very little is known about transcriptional regulation of this process during bacterial infection. to gain insight into the molecular processes that facilitate resolution of infecti ... | 2003 | 12960399 |
| influence of cadmium and zinc on the mice resistance to listeria monocytogenes infection. | the objective of this study was to examine the effect of chronic exposure to cadmium and zinc on the mice resistance to experimental listeria monocytogenes infection. | 2003 | 12960457 |
| surviving the acid test: responses of gram-positive bacteria to low ph. | gram-positive bacteria possess a myriad of acid resistance systems that can help them to overcome the challenge posed by different acidic environments. in this review the most common mechanisms are described: i.e., the use of proton pumps, the protection or repair of macromolecules, cell membrane changes, production of alkali, induction of pathways by transcriptional regulators, alteration of metabolism, and the role of cell density and cell signaling. we also discuss the responses of listeria m ... | 2003 | 12966143 |
| [listeria pathogenicity factors and their role in pathogenesis and laboratory diagnosis of listeriosis]. | the mechanisms of relationships between listeria and eukaryotic cells on the level of the main biomolecules are analyzed. these mechanisms determine the penetration and multiplication of listeria and their importance for the pathogenesis of listeriosis. the theoretical and practical aspects of the regulation of the expression of pathogenicity factors in listeria, connected with the activation of the regulatory protein prfa and autoregulatory mechanisms, are considered. the elimination of the aut ... | 2003 | 12966873 |
| expression of cellular antigens of listeria monocytogenes that react with monoclonal antibodies c11e9 and em-7g1 under acid-, salt- or temperature-induced stress environments. | to study the expression of cellular antigens of listeria monocytogenes that react with monoclonal antibodies (mabs) c11e9 and em-7g1 under acid-, salt- or temperature-induced stress environments. | 2003 | 12969290 |
| betaine and carnitine uptake systems in listeria monocytogenes affect growth and survival in foods and during infection. | to establish the relative importance of the osmo- and cryoprotective compounds glycine betaine and carnitine, and their transporters, for listerial growth and survival, in foods and during infection. | 2003 | 12969299 |
| a comparative heat inactivation study of indigenous microflora in beef with that of listeria monocytogenes, salmonella serotypes and escherichia coli o157:h7. | thermal inactivation of a mixture of five strains of listeria monocytogenes, four strains of escherichia coli o157:h7 and eight serotypes of salmonella were compared with that of indigenous microflora in 75% lean ground beef. | 2003 | 12969491 |
| quantification of listeria spp. contamination on shell and flesh of cooked black tiger prawns (penaeus monodon). | to quantify listeria levels on the shell and flesh of artificially contaminated cooked prawns after peeling, and determine the efficacy of listeria innocua as a model for l. monocytogenes in this system. | 2003 | 12969494 |
| a novel sensor of nadh/nad+ redox poise in streptomyces coelicolor a3(2). | we describe the identification of rex, a novel redox-sensing repressor that appears to be widespread among gram-positive bacteria. in streptomyces coelicolor rex binds to operator (rop) sites located upstream of several respiratory genes, including the cydabcd and rex-hemacd operons. the dna-binding activity of rex appears to be controlled by the redox poise of the nadh/nad+ pool. using electromobility shift and surface plasmon resonance assays we show that nadh, but not nad+, inhibits the dna-b ... | 2003 | 12970197 |
| intracellular accumulation and activity of ampicillin used as free drug and as its phthalimidomethyl or pivaloyloxymethyl ester (pivampicillin) against listeria monocytogenes in j774 macrophages. | to determine the intracellular accumulation in a macrophage cell line of ampicillin and ampicillin esters, and to measure their activity against intracellular listeria monocytogenes. | 2003 | 12972457 |
| maturation of lipoproteins by type ii signal peptidase is required for phagosomal escape of listeria monocytogenes. | lipoproteins of gram-positive bacteria are involved in a broad range of functions such as substrate binding and transport, antibiotic resistance, cell signaling, or protein export and folding. lipoproteins are also known to initiate both innate and adaptative immune responses. however, their role in the pathogenicity of intracellular microorganisms is yet poorly understood. in listeria monocytogenes, a gram-positive facultative intracellular human pathogen, surface proteins have important roles ... | 2003 | 12975369 |
| susceptibility of listeria monocytogenes to antimicrobial peptides. | we assessed the susceptibility of several pathogenic and non-pathogenic listeria species to antimicrobial peptides of animal and plant origin. human defensins and thionins were highly inhibitory, whereas protamine, snakin and magainin showed an intermediate effect. a temperature dependence in the activity of potato defensin was observed for listeria monocytogenes and listeria ivanovii. prfa* l. monocytogenes mutants, that overexpress constitutively prfa-dependent genes, were sensitive to the pep ... | 2003 | 13129614 |
| listeria monocytogenes sigma b regulates stress response and virulence functions. | while the stress-responsive alternative sigma factor sigma(b) has been identified in different species of bacillus, listeria, and staphylococcus, the sigma(b) regulon has been extensively characterized only in b. subtilis. we combined biocomputing and microarray-based strategies to identify sigma(b)-dependent genes in the facultative intracellular pathogen listeria monocytogenes. hidden markov model (hmm)-based searches identified 170 candidate sigma(b)-dependent promoter sequences in the strain ... | 2003 | 13129943 |
| biomimetic systems for studying actin-based motility. | actin polymerization provides a major driving force for eukaryotic cell motility. successive intercalation of monomeric actin subunits between the plasma membrane and the filamentous actin network results in protrusions of the membrane enabling the cell to move or to change shape. one of the challenges in understanding eukaryotic cell motility is to dissect the elementary biochemical and biophysical steps that link actin polymerization to mechanical force generation. recently, significant progre ... | 2003 | 13678615 |
| t cell reactivity to listeria monocytogenes amongst us. | in the last two decades, listeriosis, caused by the intracellular pathogen listeria monocytogenes, became one of the most concerning food-born infections. although it had been known before as an infectious disease of limited importance, listeriosis was brought into attention of scientists due to the frequent outbreaks recently reported. despite the major progress made towards understanding the mechanisms of virulence of l. monocytogenes, our current knowledge into the process of listeria-associa ... | 2003 | 16008146 |
| foodborne enteric infections. | foodborne infections are estimated to affect one in four americans each year. most these (67%) are caused by the norwalk-like viruses, but campylobacter and nontyphoidal salmonellae together account for about one fourth of cases of illness in which a pathogen can be detected. less common bacterial infections, such as with listeria monocytogenes and the shiga toxin-producing escherichia coli, cause fewer infections but are important because of their severe complications or high mortality rate, or ... | 2003 | 15699888 |
| [listeria disease. the first 2 cases reported at the principal hospital of dakar]. | infection disease due to listeria monocytogenes, which is a ubiquitous positive gram bacillus to the essentially alimentary transmission, listeriosis happens on patients presenting an immunodeficiency. the authors report the two first cases of listeriosis diagnosed at hopital principal de dakar. the first case was observed on a 73 years old man, hospitalised for a feverish coma scored at 9 using glasgow scale, with neither meningitis syndrome, nor sign of neurological localisation. the analysis ... | 2003 | 15776652 |
| behaviour of listeria monocytogenes in raw sausages (merguez) in presence of a bacteriocin-producing lactococcal strain as a protective culture. | the effectiveness of a bacteriocin produced by lactococcus lactis subsp. lactis m in reducing population level and growth of listeria monocytogenes atcc 7644 in fermented merguez sausage was examined. two different formulas (with or without added nitrites) were assayed and predetermined numbers of listeria (ca 10(6) cfu g(-1)) were added to sausage mixture. the effect of in situ production of the bacteriocin by lactococcus lactis m on listeria monocytogenes atcc 7644 during fermentation and stor ... | 2003 | 22062517 |
| characterization of lactic acid bacteria isolated from a greek dry-fermented sausage in respect of their technological and probiotic properties. | a total of 147 lactic acid bacteria was isolated from two types of naturally fermented dry sausages at four different stages of the ripening process studied in order to select the most suitable strains according to their technological characteristics including probiotic properties and antimicrobial activity against food-borne pathogens. identification of the isolates revealed that 90% were lactobacilli, 4% enterococci, 3% pediococcus sp. and sporadic isolates of weissella viridescens, leuconosto ... | 2003 | 22063449 |
| evaluation of sodium lactate as a replacement for conventional chemical preservatives in comminuted sausages inoculated with listeria monocytogenes. | sodium lactate (sl) as a potential replacer for potassium sorbate (ps) or sodium benzoate (sb) in comminuted sausages was evaluated. sausages manufactured with 3.3% sl were compared with a control and 0.05 or 0.1% of ps and sb with regard to its influence on changes of chemical composition, physico-chemical and textural properties, and the growth of inoculated listeria monocytogenes (lm) stored at 4 °c for up to 8 weeks. the sausages contained 62-64% moisture, 15-17% fat and 12-14% protein with ... | 2003 | 22063246 |
| effect of citric acid on the radiation resistance of listeria monocytogenes and frankfurter quality factors. | listeria monocytogenes is a common contaminant of ready-to-eat meat products, including frankfurters. ionizing (gamma) radiation can eliminate l. monocytogenes from frankfurters. citric acid (ca) is an antioxidant synergist and anti-microbial agent that can be applied to the surfaces of cured meat products prior to packaging. the effect of ca on the radiation resistance of l. monocytogenes that was surface-inoculated onto frankfurters was determined. the d(10) values, the radiation doses require ... | 2003 | 22062395 |
| antioxidant and antimicrobial effects of four garlic-derived organosulfur compounds in ground beef. | the antioxidant and antimicrobial protection of diallyl sulfide (das), diallyl disulfide (dads), s-ethyl cysteine (sec), n-acetyl cysteine (nac) in ground beef against discoloration, lipid oxidation and microbial contamination were studied. the exogenous addition of these garlic-derived organosulfur compounds significantly delayed both oxymyoglobin and lipid oxidations (p<0.05). the antioxidant protection from these organosulfur compounds was dose-dependent (p<0.05), and showed significantly gre ... | 2003 | 22061980 |
| the effects of sodium lactate and starter cultures on ph, lactic acid bacteria, listeria monocytogenes and salmonella spp. levels in pure chicken dry fermented sausage. | two starter cultures (a and b) and seven sodium lactate concentrations were evaluated for chicken raw dry-fermented sausage processing. starter culture b contained more lactic acid bacteria and less staphylococci than starter a. their effects on acidification and inhibition of pathogens (listeria monocytogenes and salmonella spp.) were monitored. starter culture b grew faster and was less inhibited by sodium lactate, thus inducting a faster and more important ph drop into the sausages. with lowe ... | 2003 | 22063699 |
| evaluation of the potential of meat born lactic acid bacteria for use as protective cultures in the biopreservation of cooked meat products. | in this study, 97 lactic acid bacteria were subjected to a step-by-step screening and characterisation to search for potential protective cultures to be used in the cooked cured meat industry. strains were first tested on their homofermentative and psychrotrophic character and salt tolerance. secondly, the antibacterial capacities towards listeria monocytogenes, leuconostoc mesenteroides, leuconostoc carnosum and brochotrix thermosphacta were determined in an agar spot test. finally, 12 selected ... | 2003 | 24757784 |
| listeria monocytogenes and inflammatory bowel diseasedetection of listeria species in intestinal mucosal biopsies by real-time pcr. | 2003 | 28248597 | |
| clinical presentation of acute bacterial meningitis in qatar. | to study the changes in the epidemiology of bacterial meningitis in the era of the hemophilus influenzae (h. influenzae) type b vaccine and pneumoccous resistance. | 2002 | 23978859 |
| cerebellar abscess due to listeria monocytogenes. | brain abscess due to listeria monocytogenes mainly involves the cerebral hemispheres. cerebellar abscess is an infrequent event, which could lead to rapid neurological deterioration if unrecognized. we present a case of multiple brain stem and cerebellar abscesses in a previously healthy individual exposed to unpasteurized milk. this is the 2nd case of cerebellar abscess due to listeria monocytogenes reported in the english literature. the diagnosis of listeria monocytogenes was made in the surg ... | 2002 | 23978925 |
| new mild technologies in meat processing: high pressure as a model technology. | as a consequence of market globalization, the production and manufacture of meat products is at a stage of innovative dynamics. consumers demand high quality and convenient meat products, with natural flavour and taste, and very much appreciate the fresh appearance of minimally processed food. to harmonize or to blend all these demands without compromising safety, it is necessary to implement new preservation technologies in the meat industry and in the food industry in general. high hydrostatic ... | 2002 | 22061612 |
| inhibition of listeria monocytogenes by a bacteriocinogenic lactobacillus sake strain in modified atmosphere-packaged brazilian sausage. | lactobacillus sake 2a is a bacteriocinogenic strain isolated from "lingüiça frescal", a brazilian sausage. the combined effect of modified-atmosphere (ma) packaging (100% co(2) and 50% co(2)/50% n(2)) and addition of l. sake 2a on inhibition of growth of listeria monocytogenes was evaluated in "lingüiça" stored at 6 °c. by the end of the first week, the inhibition of l. monocytogenes due to ma was significant (p?0.05) while the presence of l. sake 2a did not influence significantly the growth of ... | 2002 | 22061076 |
| bacteriological safety issues in red meat and ready-to-eat meat products, as well as control measures. | the importance of eschericha coli o157, listeria monocytogenes and salmonella typhimurium dt104 as meat-borne pathogens is well established. pathogenic bacteria such as aeromonas spp., arcobacter spp., psychrotrophic bacillus cereus, campylobacter spp., clostridium botulinum and non-invasive listeria monocytogenes can be regarded as rookies, but not yet firmly associated with today's production of red meat and meat products. the development of pcr and other dna-based techniques will shed new lig ... | 2002 | 22061614 |
| ribdb: an srs based infrastructure for realis. | the realis project is an eu-funded consortium for the post genomic analysis of the food pathogen listeria monocytogenes. the data generated by the consortium members is stored under the ribdb database, a system built using srs which integrates consortium data, public databases, and applications for analysis. ribdb is available to all consortium members through a web server, with the option of installing a local mirror of the main server for local analysis. | 2002 | 18628878 |
| realis: postgenomic analysis of listeria monocytogenes. | listeria monocytogenes is a remarkably successful food-borne pathogen. it is capable a) of surviving and proliferating under conditions that exist within the food chain, such as at low temperatures, high salt and low ph and b) of colonizing animal host tissues after ingestion of contaminated food, causing opportunistic infections mainly, but not exclusively, in immunocompromised hosts. the ultimate goals of realis are two fold: firstly, it aims to completely decipher all genes required for survi ... | 2002 | 18628879 |
| intra-abdominal abscess caused by listeria monocytogenes in a patient with acquired hemolytic anemia and thrombocytopenia. | we report a case of an intra-abdominal abscess caused by listeria monocytogenes in a postoperative patient with evans syndrome (acquired hemolytic anemia and thrombocytopenia). focal infections with l. monocytogenes are uncommon but have been reported in immunocompromised patients. a few cases of liver abscess in diabetic patients have also been reported. the present case is significant because of the paucity of previously described focal intra-abdominal infections caused by l. monocytogenes, pa ... | 2002 | 12540008 |
| [analysis of specific nucleotide sequences. dna biosensors]. | information about common molecular-biological approaches for the determination of the specific nucleotide sequences in genetic materials was given in the review. main attention was paid to consideration of the ways for dna biosensor creation. the information about the types of such biosensors was presented in detail and characteristics of the developed devices were cited. separately the question about the use of the instrumental analytical approaches for the identification of genetic materials o ... | 2002 | 12924012 |
| [evaluation of the activity of probiotic cultures over listeria monocytogenes during the production and storage of yogurt]. | the effect of probiotic cultures over listeria monocytogenes during the production and storage of yogurt was evaluated. a yogurt mixture (10.6% non-fat solid liquids, 3% fat and 0.3% gelatin) was prepared, homogenized and pasteurized. yogurt was inoculated with 0, 10(2), 10(4) and 10(6) cfu/ml of l. monocytogenes and 0.02% of traditional lactic culture yc 180 (streptococcus thermophilus and lactobacillus bulgaricus) and probiotic culture aby-1 (bifidobacterium longum, b. bifidum, b, infantis, la ... | 2002 | 12868278 |
| [neural listeriosis presenting as leptomeningitis: a case report]. | the listeria monocytogenes infection is rare and difficult to diagnosis. however, it is associated with a high mortality in adults. a case is presented of a 78-year-old woman with an immunological deficit following viral infection (herpes zoster). | 2002 | 12715698 |
| immunostimulatory activity of aminoalkyl glucosaminide 4-phosphates (agps): induction of protective innate immune responses by rc-524 and rc-529. | earlier we showed that the structural requirements for adjuvanticity among the aminoalkyl glucosaminide 4-phosphate (agp) class of synthetic immunostimulants may be less strict than those for other endotoxic activities, including the induction of nitric oxide synthase in murine macrophages and cytokine production in human whole blood. the known role of nitric oxide and pro-inflammatory cytokines in the activation of host defenses against infection prompted us to examine the ability of certain ag ... | 2002 | 12697089 |
| [inflammatory bowel disease--do microorganisms play a role?]. | this review focuses on the potential pathogenic role of microorganisms in relation to inflammatory bowel diseases, i.e. crohn's disease and ulcerative colitis. pathogenic microorganism such as mycobacterium paratuberculosis, measles and mumps viruses, epstein-barr virus, and listeria monocytogenes are discussed, as well as involvement of the normal intestinal flora. furthermore, the influence of microorganisms in experimental animal colitis models is discussed. the available results are inconclu ... | 2002 | 12553118 |
| adult community acquired bacterial meningitis in a singaporean teaching hospital. a seven-year overview (1993-2000). | the objective of this study is to describe the bacteriological, clinical and laboratory features of community acquired bacterial meningitis in adults admitted to a singapore tertiary-care hospital. | 2002 | 12693768 |
| effects of gamma ray and electron-beam irradiations on survival of anaerobic and facultatively anaerobic bacteria. | an extension of the approval for food irradiation is desired due to the increase in the incidence of food poisoning in the world. one anaerobic (clostridium perfringens) and four facultatively anaerobic (bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, and salmonella enteritidis) bacteria irradiated with gamma ray or electron beam (e-beam) were tested in terms of survival on agar under packaging atmosphere. using pouch pack, effects of two irradiations on survival of anaerobic ... | 2002 | 12638185 |
| [isolation and identification of listeria monocytogenes and listeria spp. in dry sausages obtained in markets in the city of la plata, argentina]. | a total of 60 samples of dry sausages were analyzed (50 of "salami" and 10 of "chorizo" "candelario" type) obtained at random in markets authorized for their commercialization, for the purpose of evidencing the presence of bacteria of the genus listeria (listeria monocytogenes and listeria spp.). the results obtained in salami were the following: 10 (20%) isolates of listeria spp., were characterized: 1 (2%) strain as l. monocytogenes type 1, 7 (14%) strains as l. innocua, 2 (4%) strains as l. w ... | 2002 | 12600006 |
| [studies on the effect of combined treatment of irradiation with vacuum packaging on ready-to-eat meat products contaminated with listeria monocytogenes and spoilage bacteria]. | altogether one hundred and fifty samples of beijing roast duck, roast chicken and cooked meat products inoculated with approximately 1.5 x 10(3)-2.3 x 10(3) cfu/g l. monocytogenes are packed under vacuum and are irradiated with 60co radiation at doses of 1.0, 2.0, 2.5 and 3 kgy. the results showed that l. monocytogenes in samples can be eliminated by a dose of 2.5 kgy. the resistance of the model strain 54004 to irradiation is stranger than three isolates (x20, g4 and g2). in addition, the spoil ... | 2002 | 12600048 |
| [an elderly case with listeria monocytogenes sepsis and pulmonary non-tuberculous mycobacterial infection]. | a 88-year-old woman, who had lived in a nursing home, was admitted to our hospital because of the suspicion of pulmonary tuberculosis. she had a cough, fever and diarrhea on admission. she suffered from sepsis because listeria monocytogenes was isolated from only the blood culture twice. we immediately administered imipenem/cilastatin to her on admission. she simultaneously had pulmonary non-tuberculous mycobacterial infection because the chest roentgenogram showed a cavity in the right upper lu ... | 2002 | 12607349 |
| effect of reuterin produced by lactobacillus reuteri on the surface of sausages to inhibit the growth of listeria monocytogenes and salmonella spp. | reuterin is a bacteriocin produced by some strains of lactobacillus reuteri. the strain used in this study was isolated from raw milk from a dairy farm nearby ankara. beef sausage is a long years produced bratwurst style meat product in turkey, as well as in some other countries in the mediterranean region. sausages are produced by raw meat; sometimes lactic starter cultures are added or spontaneous fermentation is employed. the production and storage conditions of the product promotes the growt ... | 2002 | 12577589 |
| inhibition of listeria monocytogenes lmg10470 by plantaricin ug1 in vitro and in beef meat. | the inhibition of listeria monocytogenes lmg 10470 occurred due to plantaricin ug1, but not to lactic acid produced by lactobacillus plantarum ug1 or its negatie variant (bac). partially purified plantaricin ug1 had a higher antilisterial activity in vitro and in meat than lactobacillus plantarum ug1 culture. plantaricin ug1 activity was higher in brain heart infusion (bhi) broth than in minced meat. the inhibitory effect of plantaricin ug1 against l. monocytogenes lmg10470 was dependent on its ... | 2002 | 12577590 |
| structure of internalin, a major invasion protein of listeria monocytogenes, in complex with its human receptor e-cadherin. | listeria monocytogenes, a food-borne bacterial pathogen, enters mammalian cells by inducing its own phagocytosis. the listerial protein internalin (inla) mediates bacterial adhesion and invasion of epithelial cells in the human intestine through specific interaction with its host cell receptor e-cadherin. we present the crystal structures of the functional domain of inla alone and in a complex with the extracellular, n-terminal domain of human e-cadherin (hec1). the leucine rich repeat (lrr) dom ... | 2002 | 12526809 |
| regulatory cd4+cd25+ t cells restrict memory cd8+ t cell responses. | cd4+ t cell help is important for the generation of cd8+ t cell responses. we used depleting anti-cd4 mab to analyze the role of cd4+ t cells for memory cd8+ t cell responses after secondary infection of mice with the intracellular bacterium listeria monocytogenes, or after boost immunization by specific peptide or dna vaccination. surprisingly, anti-cd4 mab treatment during secondary cd8+ t cell responses markedly enlarged the population size of antigen-specific cd8+ t cells. after boost immuni ... | 2002 | 12486101 |
| listeria monocytogenes phosphatidylinositol-specific phospholipase c: activation and allostery. | the animal and human pathogen listeria monocytogenes secretes several virulence factors, including a phosphatidylinositol-specific phospholipase c (pi-plc). sufficient quantities of l. monocytogenes pi-plc for biophysical studies were obtained by overexpression of the enzyme in escherichia coli. the purified pi-plc was examined in enzyme kinetics experiments using a new fluorogenic substrate, methyl-flip. methyl-flip is a water-soluble monomeric substrate cleaved in a manner similar to the natur ... | 2002 | 12488013 |
| fda and fsis issue listeria health advisory. | 2002 | 12523285 | |
| cpg-odn-induced inflammation is sufficient to cause t-cell-mediated autoaggression against hepatocytes. | autoimmune diseases are often associated with microbial infections. molecular mimicry between microbial antigens and self-epitopes has been suggested as a mechanism for breaking self-tolerance and induction of autoimmunity. since infections also cause inflammatory responses we explored the role of local inflammation in organ-specific autoimmunity. for this purpose, transgenic mice were used expressing the mhc class i molecule kb exclusively on hepatocytes. these mice exhibit kb-specific toleranc ... | 2002 | 12516551 |
| bacterial contamination of cucumber fruit through adhesion. | in this study, the adhesion of bacteria to fresh cucumber surfaces in aqueous suspension was shown to be dependent on time of incubation, inoculum species and concentration, and temperature. the adhesion of bacteria to the fruit in wash water was less extensive at lower temperatures and shorter exposure times. various species of bacteria were adsorbed to cucumber surfaces in the following relative order: salmonella typhimurium > staphylococcus aureus > lactobacillus plantarum > listeria monocyto ... | 2002 | 12495005 |
| incidence and susceptibility to antimicrobial agents of listeria spp. and listeria monocytogenes isolated from poultry carcasses in porto, portugal. | the occurrence of listeria spp. and listeria monocytogenes in 63 samples of portuguese poultry carcasses obtained from two local butcher shops and one canteen in the city of porto, portugal, and the susceptibility of these bacteria to antimicrobial agents allowed for use in human or animal therapeutics were evaluated. all poultry samples were contaminated with listeria spp., and l. monocytogenes was isolated from 41% (26 of 63) of the samples. other listeria species, including l. innocua, l. wel ... | 2002 | 12495006 |
| microbial antagonists of foodborne pathogens on fresh, minimally processed vegetables. | on many types of raw or minimally processed foods, the bacterial microbiota is often composed of mixed species. the activities of one bacterial species may influence the growth and activities of others that are present. the objective of this project was to evaluate the microbial composition of fresh and minimally processed vegetables to determine if naturally occurring bacteria on produce are competitive with or antagonistic to potentially encountered pathogens. naturally occurring bacteria were ... | 2002 | 12495009 |
| death of salmonella, escherichia coli o157:h7, and listeria monocytogenes in garlic butter as affected by storage temperature. | garlic is known to have antimicrobial activity against several spoilage and pathogenic bacteria. however, the fate of salmonella, escherichia coli o157:h7, and listeria monocytogenes in garlic butter has not been reported. this study was undertaken to determine the viability of these organisms in garlic butter as affected by the type of raw minced garlic added to the butter, storage temperature, and storage time. unsalted butter at 40 degrees c was combined with raw minced jumbo, elephant, or sm ... | 2002 | 12495019 |
| optimization of a culture technique for the isolation of listeria monocytogenes from faecal samples. | a culture technique employing cold enrichment at 4 degrees c followed by selective enrichment and plating at higher temperatures (30 degrees c) was used to isolate listeria monocytogenes from faecal samples. the samples were held at 4 degrees c for 15 weeks and cultured weekly to assess the sensitivity of the culture after cold storage for different lengths of time. no media, listeria selective enrichment broth (lseb), nutrient broth (nb) and saline were used as cold storage medium. cold storage ... | 2002 | 12485361 |
| chemical sympathectomy increases numbers of inflammatory cells in the peritoneum early in murine listeriosis. | here, we investigated the effects of sympathectomy on systemic bacterial loads following infection with listeria monocytogenes, and on innate and specific immune responses in the peritoneum. sympathectomy decreased systemic bacterial loads, and increased the number of peritoneal leukocytes and the percentage of peritoneal macrophages three days postinfection. this suggests that sympathectomy-induced decreases systemic bacterial loads are associated with increased recruitment of inflammatory cell ... | 2002 | 12480496 |
| biocide use in the food industry and the disinfectant resistance of persistent strains of listeria monocytogenes and escherichia coli. | the aims of the project were threefold: to survey the use of disinfectants in the uk food industry; to assess the product and environmental microflora of selected food factories for the persistence of listeria monocytogenes and escherichia coli; and to determine the disinfectant resistance of any persistent strains. | 2002 | 12481836 |
| antimicrobial activity of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), compared to other quinolones, against clinical isolates from cancer patients. | the in vitro spectrum of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), was compared with that of ciprofloxacin, levofloxacin, and trovafloxacin against 736 clinical isolates from cancer patients. garenoxacin was the most active agent overall against gram-positive organisms, with potent activity against aerococcus spp., micrococcus spp., rhodococcus equi, stomatococcus mucilaginous, bacillus spp., enterococcus faecalis, listeria monocytogenes, methicillin-susceptible staphylococcus ... | 2002 | 12458127 |
| [listeria monocytogenes meningitis in the adult in spain. report of 10 cases and review of the literature]. | ten new cases of listeria monocytogenes meningitis (lmm) in the adult are here reported. also, a review is made of reported cases by spanish authors in the last 30 years, with an analysis of the predisposing conditions and mortality rate from this type of bacterial meningitis (bm) throughout the study period. | 2002 | 12459091 |
| environmental factors influencing the inactivation of listeria monocytogenes by pulsed electric fields. | to investigate the influence of the growth phase, growth temperature, storage time, ph and aw of the treatment medium on the resistance of listeria monocytogenes to pulsed electric fields (pef). | 2002 | 12460430 |
| detection of single nucleotide polymorphisms within the listeria genus using an 'asymmetric' fluorogenic probe set and fluorescence resonance energy transfer based-pcr. | we describe a novel and inexpensive fluorescence energy transfer (fret)-based pcr protocol to distinguish single nucleotide polymorphisms (snps) within the genus listeria. | 2002 | 12460435 |
| influence of interactions between temperature, ferric ammonium citrate and glycine betaine on the growth of listeria monocytogenes in a defined medium. | to investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of listeria monocytogenes in modified pine's medium (pine et al. 1989). | 2002 | 12460440 |
| adjuvanticity of an il-12 fusion protein expressed by recombinant deltag-vesicular stomatitis virus. | the remarkable immunomodulatory and adjuvant properties of ril-12 have been well described. many early studies documenting the adjuvanticity of il-12 were performed using the murine model of listeria monocytogenes infection. in this report, we describe the construction of an attenuated recombinant vesicular stomatitis virus (vsv-deltag) that encodes a single-chain il-12 fusion protein (il-12f), and the use of this virus as an expression vector to produce large quantities of il-12f. vsv-expressed ... | 2002 | 12470614 |
| cationic polypeptides are required for antibacterial activity of human airway fluid. | in a search for direct evidence leading to the biological relevance of airway secretions in innate host defense, we characterized the antibacterial function of cationic polypeptides within minimally manipulated nasal fluid. in this study, we show that cationic antimicrobial polypeptides are responsible for most of the bactericidal activity of whole nasal fluid. the removal of cationic polypeptides using a cation-exchange resin ablated the activity of nasal fluid against escherichia coli, listeri ... | 2002 | 12471133 |
| proteomic analysis of response to acid in listeria monocytogenes. | 2002 | 12474392 | |
| [effects of mercury on the course of listeria monocytogenes infection in mice]. | effects of mercury ions on the colonization of mice organs by listeria monocytogenes were investigated. it was found that single injection of 0.05 ld50 mercury ions has little effect on listeria spreading in mice internal organs. dissemination of bacteria in the liver of mice, which received 0.5 ld50, was similar to that one in control animals, which received physiologic solution, while higher numbers of bacteria were found in spleen of experimental animals at later stages of infection (after 24 ... | 2002 | 12474674 |
| [effects of aluminum ions on the resistance of mice to experimental listeria monocytogenes infection]. | this study presents evaluation of effects of aluminium ions on the development of experimental infection induced by the injection of listeria monocytogenes bacteria into growing mice. we show that single exposure of mice to 0.05 ld50 or 0.5 ld50 of aluminium ions does not affect the accumulation of bacteria in liver and spleen of experimental animals. long-term exposure of mice to aluminium ions (injection of 0.05 ld50 al3+ every 3 days for totally 6 weeks) did not slow down the increase in weig ... | 2002 | 12474775 |
| listeriosis in iceland, 1978-2000: a description of cases and molecular epidemiology. | the purpose of our study was to review all cases of listeriosis in iceland during the period 1978-2000 and to analyse the genetic relatedness of their isolates. case records of all patients in iceland with listeriosis during the period were reviewed and the isolates compared using serotyping and pulsed-field gel electrophoresis (pfge) using smai, asei and apai restriction enzymes. forty cases of listeriosis were diagnosed during the period, resulting in a mean annual incidence of 6.9 cases per m ... | 2002 | 12477323 |
| cns listeriosis confused with leptomeningeal carcinomatosis in a patient with a malignant insulinoma. | we describe a case of presumed listeria monocytogenes rhomboencephalitis, which was initially confused with leptomeningeal carcinomatosis in a patient with a malignant carcinoid tumor. long-term corticosteroid treatment and immunosuppression caused by malignancy predisposed the patient to developing listeriosis. the clinical and radiologic features of this illustrative case are described. listeriosis is an important treatable differential diagnosis in patients with malignancy presenting with neu ... | 2002 | 12478002 |
| diagnostic usefulness of diffusion-weighted magnetic resonance imaging in listerial rhombencephalitis. | 2002 | 12453092 | |
| quality improvement of kosher chilled poultry. | pathogens like listeria monocytogenes are of great concern in the poultry industry. poultry, which are subjected to the kosher slaughtering and koshering process, may introduce even higher risks than conventially slaughtered poultry due to the potential for microbial cross-contamination at several critical points in the koshering line, particularly in the chillers. the effect of microgard (mic) and nisin (nis) on reducing total microbial counts, inhibiting l. monocytogenes, and prolonging shelf ... | 2002 | 12455605 |
| bactericidal effect of gentamicin-induced membrane vesicles derived from pseudomonas aeruginosa pao1 on gram-positive bacteria. | previous studies have shown that gentamicin-induced membrane vesicles (g-mvs) from pseudomonas aeruginosa pao1 possess both the antibiotic (gentamicin) and a potent peptidoglycan hydrolase (pgase; autolysin) that is effective in killing gram-negative pathogens. this present study evaluated the therapeutic potential of g-mvs against four gram-positive bacteria. bactericidal assays and electron microscopy of thin sections revealed that bacillus subtilis 168 and staphylococcus aureus d2c were susce ... | 2002 | 12455613 |