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methodologies for the characterization of microbes in industrial environments: a review.there is growing interest in research and development to develop novel tools to study, detect, and characterize microbes and their communities in industrial environments. however, knowledge about their validity in practical industrial use is still scarce. this review describes the advantages and limitations of traditional and molecular methods used for biofilm and/or planktonic cell studies, especially those performed with listeria monocytogenes, bacillus cereus, and/or clostridium perfringens. ...200312764674
anti-oxidant properties of n-acetyl-l-cysteine do not improve the immune resistance of mice fed dietary lipids to listeria monocytogenes infection.current knowledge of the potential effects that several dietary lipids exert on immune functions indicates that these substances participate actively in the modulation of immune system by which they contribute to the improvement of the conditions of patients suffering from inflammatory disorders. however, long-chain n-3 polyunsaturated fatty acids induce an immunosuppressive status that leads to a reduction of the host natural resistance to infectious agents as well as to an enhancement of oxida ...200312765672
potential role of the epec translocated intimin receptor (tir) in host apoptotic events.apoptosis, or programmed cell death, is a well-ordered process that allows damaged or diseased cells to be removed from an organism without severe inflammatory reactions. multiple factors, including microbial infection, can induce programmed death and trigger reactions in both host and microbial cellular pathways. whereas an ultimate outcome is host cell death, these apoptotic triggering mechanisms may also facilitate microbial spread and prolong infection. to gain a better understanding of the ...200312766478
recombinant e. coli efficiently delivers antigen and maturation signals to human dendritic cells: presentation of mart1 to cd8+ t cells.the generation of tumour-specific cytotoxic t-lymphocyte (ctl) responses is the primary focus in the design of immunotherapeutic cancer vaccines. we have recently demonstrated generation of ovalbumin (ova)-specific ctls and tumour-protection in a murine tumour model using vaccination with dendritic cells (dcs) pulsed with e. coli expressing listeriolysin o (llo) and ova as a model antigen. in this system paraformaldehyde fixation of e. coli/llo provided an additional safety feature without compr ...200312767067
immune changes during acute cold/restraint stress-induced inhibition of host resistance to listeria.experiments were conducted to delineate the cellular changes modulated by acute cold/restraint stress (acrs), a physical and psychological stressor, in response to a listeria monocytogenes(lm) infection. in addition to wild type (wt) balb/c mice, cd4-deficient (cd4-/-) balb/c mice, which have no effective adaptive immunity, were used to determine the involvement of adaptive versus innate immunity. acrs-induced suppression of host resistance to lm was not observed in cd4-/- mice, suggesting the i ...200312773754
a crucial role for profilin-actin in the intracellular motility of listeria monocytogenes.we have examined the effect of covalently crosslinked profilin-actin (pxa), which closely matches the biochemical properties of ordinary profilin-actin and interferes with actin polymerization in vitro and in vivo, on listeria monocytogenes motility. pxa caused a marked reduction in bacterial motility, which was accompanied by the detachment of bacterial tails. the effect of pxa was dependent on its binding to proline-rich sequences, as shown by the inability of ph133sxa, which cannot interact w ...200312776739
t cells bite the hand that feeds them. 200312778158
molecular subtyping to detect human listeriosis clusters.we analyzed the diversity (simpson's index, d) and distribution of listeria monocytogenes in human listeriosis cases in new york state (excluding new york city) from november 1996 to june 2000 by using automated ribotyping and pulsed-field gel electrophoresis (pfge). we applied a scan statistic (p<or=0.05) to detect listeriosis clusters caused by a specific listeria monocytogenes subtype. among 131 human isolates, 34 (d=0.923) ribotypes and 74 (d=0.975) pfge types were found. nine (31% of cases) ...200312781006
protective effects of chlorella vulgaris in lead-exposed mice infected with listeria monocytogenes.chlorella vulgaris extract (cve) was examined for its chelating effects on the myelosuppression induced by lead in listeria monocytogenes-infected mice. the reduction in the number of bone marrow granulocyte-macrophage progenitors (cfu-gm) observed after the infection was more severe in the groups previously exposed to lead. extramedullar hematopoiesis, which was drastically increased after the infection, was not altered by the presence of lead. treatment with cve, given simultaneously or follow ...200312781705
the effect of growth atmosphere on the ability of listeria monocytogenes to survive exposure to acid, proteolytic enzymes and bile salts.four isolates of listeria monocytogenes from food, human and environmental sources were grown separately in broth (ph 6.0 at 8 degrees c) under atmospheres of air, 100% n(2), 40% co(2):60% n(2) or 100% co(2). exponential and stationary phase cells were harvested to determine if growth atmosphere and growth phase influenced this pathogen's ability to survive exposure to an acid environment coupled with proteolytic enzymes, and the activity of bile salts. in general, isolates were more resistant t ...200312781937
stress response of listeria monocytogenes isolated from cheese and other foods.the responses to ph and sodium chloride of four strains of listeria monocytogenes isolated from portuguese cheese, with a sodium chloride concentration of about 2% (w/v) and a ph value from 5.1 to 6.2, were studied. two isolates from meat and two clinical isolates related to food-borne listeriosis, in which the implicated food product had about 2-3.5% (w/v) sodium chloride, also were studied. the effect of temperature on ph and sodium chloride sensitivity was also determined. the results show th ...200312781943
simultaneous detection of escherichia coli o157:h7, listeria monocytogenes and salmonella strains by real-time pcr.a protocol enabling simultaneous detection of escherichia coli o157:h7, listeria monocytogenes and salmonella strains was devised and evaluated using artificially contaminated fresh produce. association of official analytical chemists (aoac)-approved polymerase chain reaction (pcr) detection methods for three human pathogens were modified to enable simultaneous and real-time detection with high throughput capability. the method includes a melting-curve analysis of pcr products, which serves as c ...200312781944
effects of a bacteriocin-like inhibitory substance from carnobacterium piscicola against human and salmon isolates of listeria monocytogenes.the aim of this study was to characterize the antagonism of a bacteriocin-like inhibitory substance (blis) produced by carnobacterium piscicola l103 against listeria monocytogenes strains isolated from salmon and human samples. the inhibitory effect of the blis was evaluated in tryptic soy agar (tsa) during different growth phases of l. monocytogenes at 5 degrees c, using the well diffusion method. also, the type of inhibition, either bacteriostatic or bactericidal of the blis in tryptic soy bro ...200312781946
rapid enumeration of listeria monocytogenes in milk using competitive pcr.competitive polymerase chain reaction (cpcr) was used to develop a direct enumeration method of listeria monocytogenes in milk. sterile milk was artificially inoculated with l. monocytogenes and dna was extracted using guanidine thiocyanate/phenol/chloroform, followed by pcr. several primers for l. monocytogenes hlya gene were tested for specific detection and dg69/dg74 primer set was selected. the primer set produced a 636-bp band from l. monocytogenes, but no band appeared from the other six l ...200312781957
haemolysin a and listeriolysin--two vaccine delivery tools for the induction of cell-mediated immunity.haemolysin a of escherichia coli and listeriolysin of listeria monocytogenes represent important bacterial virulence factors. while such cytolysins are usually the reason for morbidity and even mortality, vaccine researchers have turned haemolysin a and listeriolysin into tools for vaccine delivery. both cytolysins have found widespread application in vaccine research and are highly suitable for the elicitation of cell-mediated immunity. in this paper, we will review vaccine delivery mediated by ...200312782050
identification of listeria monocytogenes genes involved in salt and alkaline-ph tolerance.the capacity of listeria monocytogenes to tolerate salt and alkaline stresses is of particular importance, as this pathogen is often exposed to such environments during food processing and food preservation. we screened a library of tn917-lacz insertional mutants in order to identify genes involved in salt and/or alkaline tolerance. we isolated six mutants sensitive to salt stress and 12 mutants sensitive to salt and alkaline stresses. the position of the insertion of the transposon was located ...200312788708
development of a listeria monocytogenes egde partial proteome reference map and comparison with the protein profiles of food isolates.a partially annotated proteome reference map of the food pathogen listeria monocytogenes was developed for exponentially growing cells under standardized, optimal conditions by using the sequenced strain egde (serotype 1/2a) as a model organism. the map was developed by using a reproducible total protein extraction and two-dimensional (2-d) polyacrylamide gel electrophoresis analysis procedure, and it contained 33 identified proteins representing the four main protein functional classes. in orde ...200312788738
adhesion, invasion, and translocation characteristics of listeria monocytogenes serotypes in caco-2 cell and mouse models.adhesion is a crucial first step in listeria monocytogenes pathogenesis. in this study, we examined how the adhesion properties of serotypes correlate with their invasion efficiencies in a cell culture model (caco-2) and in a mouse model. adhesion characteristics of all 13 serotypes of l. monocytogenes (25 strains) were analyzed, which yielded three distinct groups (p < 0.05) with high-, medium-, and low-level-adhesion profiles. the efficiency of these strains in invading the caco-2 cell line wa ...200312788773
generation of food-grade lactococcal starters which produce the lantibiotics lacticin 3147 and lacticin 481.transconjugant lactococcal starters which produce both lantibiotics lacticin 3147 and lacticin 481 were generated via conjugation of large bacteriocin-encoding plasmids. a representative of one of the resultant strains proved more effective at killing lactobacillus fermentum and inhibiting the growth of listeria monocytogenes lo28h than either of the single bacteriocin-producing parental strains, demonstrating the potential of these transconjugants as protection cultures for food safety applicat ...200312788782
aromatic amino acids at the surface of inlb are essential for host cell invasion by listeria monocytogenes.the surface protein inlb of the pathogen listeria monocytogenes promotes invasion of this bacterium into host cells by binding to and activating the receptor tyrosine kinase met. the curved leucine-rich repeat (lrr) domain of inlb, which is essential for this process, contains a string of five surface-exposed aromatic amino acid residues positioned along its concave face. here, we show that the replacement of four of these residues (f104, w124, y170 or y214) by serine leads to a complete loss of ...200312791136
isolation of listeria monocytogenes mutants with high-level in vitro expression of host cytosol-induced gene products.the facultative intracellular bacterial pathogen listeria monocytogenes dramatically increases the expression of several key virulence factors upon entry into the host cell cytosol. acta, the protein product of which is required for cell-to-cell spread of the bacterium, is expressed at low to undetectable levels in vitro and increases in expression more than 200-fold after l. monocytogenes escape from the phagosome. to identify bacterial factors that participate in the intracellular induction of ...200312791137
report of an additional case of anti-tumor necrosis factor therapy and listeria monocytogenes infection: comment on the letter by glück et al. 200312794847
heterogeneity of virulence-related properties in listeria monocytogenes strains isolated from patients with haematological malignancies.listeria monocytogenes is an intracellular foodborne pathogen of humans and animals for which there are indications of virulence differences among strains. various virulence properties related to different phases of infection process were investigated in l. monocytogenes strains isolated from patients affected by haematological malignancies. in these isolates, besides to the clinical history, we analysed the haemolysin production, the survival to acidic ph, the ability to enter and proliferate i ...200312797902
il-12-assisted immunization generates cd4+ t cell-mediated immunity to listeria monocytogenes.mice infected with virulent listeria monocytogenes develop long-lived acquired immunity. we previously reported that acquired immunity to listeria could also be elicited by immunizing mice with non-viable listeria or listerial proteins/peptides in combination with il-12. here we show that this il-12-assisted immunization strategy was effective in class i but not in class ii mhc-deficient mice, suggesting that antigen-specific cd4(+) t cells are selectively generated using this adjuvant system. w ...200312798303
membrane filter (pore size, 0.22-0.45 micro m; thickness, 150 micro m) passing-through activity of pseudomonas aeruginosa and other bacterial species with indigenous infiltration ability.bacteria growing on mf-millipore filters (thickness, 150 micro m) passed through the underlying membrane by their infiltration activity. bacillus subtilis, staphylococcus aureus, klebsiella pneumoniae, and escherichia coli passed through a 0.45- micro m pore size filter within 48-96 h. pseudomonas aeruginosa, serratia marcescens, and listeria monocytogenes passed through a 0.3- micro m pore size filter. p. aeruginosa passed through a 0.22- micro m pore size filter. the membranes which allowed pa ...200312798998
a branched chain fatty acid promotes cold adaptation in bacteria. 200312799482
micro-assembly of functionalized particulate monolayer on c18-derivatized sio2 surfaces.this work describes a simple approach to immobilize functionalized colloidal microstructures onto a c(18)-coated sio(2) substrate via specific or non-specific bio-mediated interactions. biotinylated bovine serum albumin pre-adsorbed onto a c(18) surface was used to mediate the surface assembly of streptavidin-coated microbeads (2.8 microm), while a bare c(18) surface was used to immobilize anti-listeria antibody-coated microbeads (2.8 microm) through hydrophobic interactions. for a c(18) surface ...200312800136
survival and recovery of listeria monocytogenes on ready-to-eat meats inoculated with a desiccated and nutritionally depleted dustlike vector.dust from construction was theorized to serve as a vector for l. monocytogenes transmission to ready-to-eat (rte) meats after heat processing but before packaging. a five-strain listeria monocytogenes culture including serotype 4b was continually stressed on a sand vector under four sets of nutritionally depleted and dry conditions to simulate postprocessing contamination by dustlike particulates. the stresses included that associated with sand stored at different temperatures (10 and 22 degrees ...200312800995
acid adaptation does not promote survival or growth of listeria monocytogenes on fresh beef following acid and nonacid decontamination treatments.the objective of this study was to evaluate the survival and growth of acid-adapted and nonadapted listeria monocytogenes inoculated onto fresh beef subsequently treated with acid or nonacid solutions. beef slices (2.5 by 5 by 1 cm) from top rounds were inoculated with acid-adapted or nonadapted l. monocytogenes (4.6 to 5.0 log cfu/cm2) and either left untreated (control) or dipped for 30 s in water at 55 degrees c, water at 75 degrees c, 2% lactic acid at 55 degrees c, or 2% acetic acid at 55 d ...200312800998
ionizing radiation sensitivity of listeria monocytogenes atcc 49594 and listeria innocua atcc 51742 inoculated on endive (cichorium endiva).ionizing radiation inactivates the pathogenic bacteria that can contaminate leafy green vegetables. leaf pieces and leaf homogenate of endive (cichorium endiva) were inoculated with the pathogen listeria monocytogenes (atcc 49594) or listeria innocua (atcc 51742), a nonpathogenic surrogate bacterium. the radiation sensitivity of the two strains was similar, although l. innocua was more sensitive to the type of suspending leaf preparation. during refrigerated storage after irradiation, the popula ...200312800999
inhibition of listeria monocytogenes in ph-adjusted pasteurized liquid whole egg.although the transmission of l. monocytogenes to humans via pasteurized egg products has not been documented, l. monocytogenes and other listeria species have been isolated from commercially broken raw liquid whole egg (lwe) in both the united states and ireland. recent listeria thermal inactivation studies indicate that conventional minimal egg pasteurization processes would effect only a 2.1- to 2.7-order-of-magnitude inactivation of l. monocytogenes in lwe; thus, the margin of safety provided ...200312801000
evaluation of a select strain of lactobacillus delbrueckii subsp. lactis as a biological control agent for pathogens on fresh-cut vegetables stored at 7 degrees c.raw vegetables inoculated with selected pathogenic bacteria were treated with a strain of lactobacillus delbrueckii subsp. lactis, which was selected for its ability to produce hydrogen peroxide at refrigerated temperatures. the vegetables inoculated included broccoli, cabbage, carrots, and lettuce. each vegetable was rinsed, chopped, and stored under conditions similar to those used for ready-to-eat vegetables sold at retail. portions of each vegetable were separately inoculated with one of two ...200312801002
reduction of bacteria on pork carcasses associated with chilling method.in addition to reducing the temperature of pork carcasses immediately after slaughter and before fabrication, blast chilling (snap chill) or conventional chilling can reduce bacterial populations associated with fresh meats. however, there is little information on bacteria survival resulting from the freeze or chill injury of meat products. in this study, porcine fecal slurries with and without pathogens (listeria monocytogenes, salmonella typhimurium, and campylobacter coli) were inoculated ont ...200312801003
antimicrobial properties of commercial annatto extracts against selected pathogenic, lactic acid, and spoilage microorganisms.annatto preparations are used to impart distinctive flavor and color to foods and are a primary colorant in dairy foods such as cheese and butter. there are several reports indicating that certain fractions of the annatto plant have biological activities against microorganisms of significance in food fermentation, food preservation, and human health. however, little is reported describing the nature of the antimicrobial compound(s) or their potential presence in commercial annatto colorant prepa ...200312801012
modelling the individual cell lag phase. isolating single cells: protocol development.to develop a protocol to isolate single cells in wells of a microtitre plate, having a high certainty of individual cells, combined with a sufficient yield.200312803551
survivability and long-term preservation of bacteria in water and in phosphate-buffered saline.to evaluate the suitability of using sterile water and phosphate-buffered saline (pbs) for preservation of bacteria pathogenic to plants or humans.200312803555
enhancement of immune responses to an hiv env dna vaccine by a c-terminal segment of listeriolysin o.an effective vaccine against aids should induce both cellular and humoral immune responses. here we report that immunization of mice with a dna plasmid encoding a chimeric protein consisting of hiv89.6 env gp140 and the listeriolysin o (llo) c-terminal segment (59 amino acids) significantly enhanced both humoral and cellular immune responses against the hiv89.6 env protein. plasmid dna expression vectors with genes codon-optimized for mammalian expression were synthesized for hiv89.6 gp140 as we ...200312803999
induction of interleukin-12 production in mouse macrophages by berberine, a benzodioxoloquinolizine alkaloid, deviates cd4+ t cells from a th2 to a th1 response.in this study we investigated whether berberine-mediated induction of interleukin-12 (il-12) production in antigen-presenting cells could regulate a cytokine profile of antigen-primed cd4+ t helper (th) cells. pretreatment with berberine induced il-12 production in both macrophages and dendritic cells, and significantly increased the levels of il-12 production in lipopolysaccharide-stimulated macrophages and in cd40 ligand-stimulated dendritic cells. importantly, berberine pretreatment of macrop ...200312807487
anti-human immunodeficiency virus-gag cd8+ memory t cells generated in vitro from listeria-immunized mice.the goal of vaccination is the generation of immune memory, an immune state that permits rapid and intense recall responses to a pathogen. considerable effort is being made to understand the nature of memory t cells. we report here that by extending the length of in vitro culture following a single restimulation with specific peptide, preparations of highly enriched, highly active antigen-specific cd8+ memory t cells could be obtained. these cultures were begun with splenocytes from mice primed ...200312807492
comparative study of protective effects of chitin, chitosan, and n-acetyl chitohexaose against pseudomonas aeruginosa and listeria monocytogenes infections in mice.we conducted a comparative study of the protective effects of chitin, chitosan, and n-acetyl chitohexaose (nacos-6) against mice infected intravenously or intraperitoneally with pseudomonas aeruginosa and listeria monocytogenes. mice pretreated with chitin, chitosan, and nacos-6 showed resistance to intraperitoneal infections by both microbes. only mice pretreated with chitin and chitosan showed resistance to intravenous infections by both microbes. the number, active oxygen generation, and myel ...200312808311
cytosolic delivery of antisense oligonucleotides by listeriolysin o-containing liposomes.antisense oligodeoxynucleotides (odns) possess great potential as sequence-specific therapeutic agents. sufficient concentrations of intact odn must bypass membrane barriers and access the cytosol and nucleus, for odns to be therapeutically effective. a cytosolic delivery strategy was designed to improve the efficiency of odn delivery in bone-marrow-derived macrophages. this liposome-based formulation utilizes listeriolysin o (llo), the endosomolytic hemolysin from listeria monocytogenes, to med ...200312808441
the effect of processed meat and meat starter cultures on gastrointestinal colonization and virulence of listeria monocytogenes in mice.listeria monocytogenes is a foodborne pathogen of major concern to the food industry in general and the meat industry in particular. the aim of this study was firstly to identify a strain of listeria that was virulent in spf balb/c mice. secondly, to investigate if a traditional meat starter culture (floracarn) and nontraditional meat starter (ntms) cultures of dairy product and human origin (lactobacillus and bifidobacteria) inhibit this pathogen in vivo. in addition, the inhibition of listeria ...200312810289
relevance of microbial interactions to predictive microbiology.microbial interaction can be ignored in predictive microbiology under most conditions. we show that interactions are only important at high population densities, using published data on inhibition of growth of listeria monocytogenes in broth. our analysis using growth models from predictive microbiology indicated that interactions only occur at population densities of approximately 10(8) cfu/ml of the protective cultures. spoilage is evident at these levels, except for fermented foods. in bacter ...200312810290
genetic variability among isolates of listeria monocytogenes from food products, clinical samples and processing environments, estimated by rapd typing.rapd analysis with four primers was used to examine the genetic relationship among 432 strains of listeria monocytogenes isolated from clinical and veterinarian cases of listeriosis, dairy, vegetable, meat- and fish-based food items, environmental samples and samples collected from one transport terminal, one poultry-processing company and four atlantic salmon-processing plants. the purpose of the study was to determine whether clinical isolates belonged to a specific genetic group, whether link ...200312810292
transglutaminase 2-/- mice reveal a phagocytosis-associated crosstalk between macrophages and apoptotic cells.tissue transglutaminase (tgase2) is a protein-crosslinking enzyme known to be associated with the in vivo apoptosis program. here we report that apoptosis could be induced in tgase2-/- mice; however, the clearance of apoptotic cells was defective during the involution of thymus elicited by dexamethasone, anti-cd3 antibody, or gamma-irradiation, and in the liver after induced hyperplasia. the lack of tgase2 prevented the production of active transforming growth factor-beta1 in macrophages exposed ...200312810961
cytoplasmic bacteria can be targets for autophagy.autophagy is an important constitutive cellular process involved in size regulation, protein turnover and the removal of malformed or superfluous subcellular components. the process involves the sequestration of cytoplasm and organelles into double-membrane autophagic vacuoles for subsequent breakdown within lysosomes. in this work, we demonstrate that the intracellular pathogen listeria monocytogenes can also be a target for autophagy. if infected macrophages are treated with chloramphenicol af ...200312814436
the uterine nk cell population requires il-15 but these cells are not required for pregnancy nor the resolution of a listeria monocytogenes infection.during pregnancy in mice, uterine natural killer (unk) cells abundantly accumulate on the mesometrial side of the placenta. in this study, we show that the presence of both mature and immature unk cells requires il-15. bone marrow transplantation of nk cell-negative mice due to null mutations in the recombination-activating gene (rag) 2/common cytokine receptor gamma-chain (rag2(-/-)gamma(c)(-/-)) genes indicated that unk cells originate from the bone marrow and require il-15 to develop. nk cell ...200312816981
class ia mhc-deficient balb/c mice generate cd8+ t cell-mediated protective immunity against listeria monocytogenes infection.cd8(+) t cells are required for protective immunity against intracellular pathogens such as listeria monocytogenes. in this study, we used class ia mhc-deficient mice, which have a severe reduction in circulating cd8(+) t cells, to determine the protective capacity of class ib mhc-restricted t cells during l. monocytogenes infection. the k(b-/-)d(b-/-) mutation was backcrossed onto a c.b10 (balb/c congenic at h-2 locus with c57bl/10) background, because balb/c mice are more susceptible to lister ...200312817010
cd44-regulated intracellular proliferation of listeria monocytogenes.cd44 has been implicated in immune and inflammatory processes. we have analyzed the role of cd44 in the outcome of listeria monocytogenes infection in murine bone marrow-derived macrophages (bmm). surprisingly, a dramatically decreased intracellular survival of l. monocytogenes was observed in cd44(-/-) bmm. cd44(-/-) heart or lung fibroblast cultures also showed reduced bacterial levels. moreover, livers from cd44(-/-)-infected mice showed diminished levels of l. monocytogenes. in contrast, int ...200312819101
listeriosis in second trimester of pregnancy: case report from india.although listeria monocytogenes infection occurs in sporadic and epidemic forms throughout the world, there are certain countries (especially asian countries) that have reported only a few cases or failed to report even a single case. during her third visit at 17(+5) weeks of gestation, a 22-year-old primigravida presented with the complaint of an acute painful abdomen, leaking per vaginum and low-grade fever for the 2 preceding days. on ultrasonography, a single live fetus with no amniotic flui ...200312824687
successful replacement of systemic immunosuppression by local graft irradiation in the management of listeria meningitis. 200312826148
infection with listeria monocytogenes following orthotopic liver transplantation: case report and review of the literature.infection with listeria monocytogenes is rare with a reported annual incidence of 4.4 cases/million individuals. epidemiological data have identified certain groups to be higher risk of developing listeriosis, including neonates, pregnant women, adults older than 60 years of age, individuals afflicted with hematologic malignancies, acquired immunodeficicency syndrome, cirrhosis, and those receiving corticosteroid therapy and organ transplants. within this last group, multiple cases have been des ...200312826201
pathogen, host and environmental factors contributing to the pathogenesis of listeriosis.listeriosis is a severe human and animal disease caused by two species of pathogenic bacteria from the genus listeria, l. monocytogenes and l. ivanovii. in humans, listeriosis is overwhelmingly a foodborne disease, yet much remains to be learned regarding the transmission dynamics of pathogenic listeria from the environment, through food, to humans. similarly, our understanding of the various host, pathogen and environmental factors that impact the pathogenesis of listeriosis at the cellular and ...200312827280
the smtb/arsr family of metalloregulatory transcriptional repressors: structural insights into prokaryotic metal resistance.the smtb/arsr family of prokaryotic metalloregulatory transcriptional repressors represses the expression of operons linked to stress-inducing concentrations of di- and multivalent heavy metal ions. derepression results from direct binding of metal ions by these homodimeric "metal sensor" proteins. an evolutionary analysis, coupled with comparative structural and spectroscopic studies of six smtb/arsr family members, suggests a unifying "theme and variations" model, in which individual members h ...200312829264
tumor cell killing enabled by listeriolysin o-liposome-mediated delivery of the protein toxin gelonin.gelonin is a type i plant toxin that has potential as an effective anti-tumor agent by virtue of its enzymatic capacity to inactivate ribosomes and arrest protein synthesis, thereby effectively limiting the growth of cancer cells. being a hydrophilic macromolecule, however, gelonin has limited access to its target subcellular compartment, the cytosol; it is effectively plasma membrane-impermeant and subject to rapid degradation within endosomes and lysosomes upon cellular uptake as it lacks the ...200312832408
adsa foundation scholar award--an integrated science-based approach to dairy food safety: listeria monocytogenes as a model system.transmission of food- and milkborne pathogens often involves complex interactions among the pathogen, the environment, and one or multiple host species. a complete understanding of these interactions is critical to allow the development of science-based, effective intervention strategies for foodborne infectious diseases. this article summarizes our studies on the transmission, ecology, pathogenesis and population genetics of listeria monocytogenes, which we have used as model for a food- and mi ...200312836921
effects of carbon dioxide on bacterial growth parameters in milk as measured by conductivity.inhibition of bacterial growth by dissolved carbon dioxide (co2) has been well established in many foods including dairy foods. however, the effects of dissolved co2 on specific growth parameters such as length of lag phase, time to maximum growth rate, and numbers of organisms at the stationary phase have not been quantified for organisms of concern in milk. the effect of dissolved co2 concentrations of 0.6 to 61.4 mm on specific bacterial growth parameters in raw or single organism inoculated ...200312836927
structural study of binding of flagellin by toll-like receptor 5.in order to predict the binding regions within the complex formed by toll-like receptor 5 (tlr-5) and flagellin, a complementary hydropathy between the two proteins was sought. a region common to the flagellins of salmonella enterica serovar typhimurium, pseudomonas aeruginosa, and listeria monocytogenes was shown to be hydropathically complementary to the 552-to-561 fragment of tlr-5, whose sequence is eildisrnql. the hydrophobicity profile of this region is shared with flagellins of 377 bacter ...200312837800
salmonella enterica serovar typhimurium and listeria monocytogenes acid tolerance response induced by organic acids at 20 degrees c: optimization and modeling.an acid tolerance response (atr) has been demonstrated in listeria monocytogenes and salmonella enterica serovar typhimurium in response to low ph poised (i.e., adapted) with acetic or lactic acids at 20 degrees c and modeled by using dynamic differential equations. the atr was not immediate or prolonged, and optimization occurred after exposure of l. monocytogenes for 3 h at ph 5.5 poised with acetic acid and for 2 h at ph 5.5 poised with lactic acid and after exposure of s. enterica serovar ty ...200312839765
temporal stability and biodiversity of two complex antilisterial cheese-ripening microbial consortia.the temporal stability and diversity of bacterial species composition as well as the antilisterial potential of two different, complex, and undefined microbial consortia from red-smear soft cheeses were investigated. samples were collected twice, at 6-month intervals, from each of two food producers, and a total of 400 bacterial isolates were identified by fourier-transform infrared spectroscopy and 16s ribosomal dna sequence analysis. coryneform bacteria represented the majority of the isolates ...200312839776
early-onset listeriosis in prematurity.listeria monocytogenes has been recognized as a human pathogen for more than 70 years. it causes illness mainly in pregnant women, newborns, elderly, and immunocompromised persons. although l. monocytogenes is a relatively uncommon pathogen in neonates, it can cause considerable morbidity and mortality in this age group, especially in the early-onset form of the disease. in taiwan, neonatal listeriosis is rarely reported. we report one case of a premature newborn with early-onset listeria sepsis ...200312845854
cutting edge: protective cell-mediated immunity to listeria monocytogenes in the absence of myeloid differentiation factor 88.in addition to their role in triggering innate immune responses, toll-like receptors are proposed to play a key role in linking the innate and adaptive arms of the immune response. the majority of cellular responses downstream of toll-like receptors are mediated through the adapter molecule myeloid differentiation factor 88 (myd88), and mice with a targeted deletion of myd88 are highly susceptible to bacterial infections, including primary infection with listeria monocytogenes (lm). in contrast, ...200312847214
identification of uteroglobin-related protein 1 and macrophage scavenger receptor with collagenous structure as a lung-specific ligand-receptor pair.high in normal (hin)-1 is a secreted protein highly expressed in normal breast epithelium and down-regulated in breast carcinomas. by searching genbank expressed sequence tag databases, we identified hin-2, a protein homologous to hin-1. hin-2 is identical with a recently identified protein called uteroglobin-related protein 1 (ugrp1). northern blot analysis demonstrated that ugrp1 is specifically expressed by lung, but not by the other tissues examined. by in situ hybridization experiments, ugr ...200312847263
endogenous bacterial endophthalmitis: a 17-year prospective series and review of 267 reported cases.endogenous bacterial endophthalmitis is a rare but serious condition that occurs when bacteria cross the blood-ocular barrier and multiply within the eye. we provide an overview of endogenous bacterial endophthalmitis by reviewing 267 reported cases and integrating this with our experience of an additional 19 cases. the majority of patients with endogenous bacterial endophthalmitis are initially misdiagnosed and many have an underlying disease known to predispose to infection. this condition is ...200312850229
design of n-substituted peptomer ligands for evh1 domains.ena/vasp proteins are implicated in cytoskeletal reorganization during actin-dependent motility processes. recruitment to subcellular sites of actin polymerization is mediated by the highly conserved n-terminal evh1 domain, which interacts with target proteins containing proline-rich motifs. the vasp evh1 domain specifically binds peptides with the consensus motif fpppp present in all its binding partners, including the listerial acta protein. previous studies have shown that the phe and first a ...200312857736
rational approaches to immune regulation.our laboratory is interested in the properties of proteins that render them immunogenic, and how such immunogenicity may be modulated in vivo. we are attempting to enhance the immune response in the design of more effective vaccines against viral diseases, such as hiv, and against tumor antigens expressed on breast, ovarian, and cervical cancer and b cell lymphomas. our main approach is to use a facultative intracellular bacterium, listeria monocytogenes, which has the unusual ability to live an ...200312857988
application of leuconostoc carnosum for biopreservation of cooked meat products.to optimize the practical use of the bacteriocin producing leuconostoc carnosum 4010 in order to inhibit the growth of listeria monocytogenes in sliced meat products.200312859754
characteristics and identification of enterocins produced by enterococcus faecium jcm 5804t.to screen bacteriocin-producing lactic acid bacteria (lab) in 52 type and reference strains, which have not previously been studied, with respect to bacteriocins, and to characterize the presence of bacteriocins.200312859761
typing of listeria monocytogenes isolates by random amplification of polymorphic dna.listeria monocytogenes is an important food-borne pathogen causing meningitis and septicaemia in newborns and immunocompromised persons, abortion and preterm labour in pregnant women. though various methods are available for typing l. monocytogenes, rapd analysis has been used for epidemiological purposes in developed countries due to its greater discriminating ability. however, as there are no published reports from india on the typing of l. monocytogenes by rapd technique the present study was ...200312866822
an improved amplified fragment length polymorphism (aflp) protocol for discrimination of listeria isolates.nine restriction enzyme combinations and 108 different primer combinations were initially tested for suitability for amplified fragment length polymorphism (aflp) analysis of listeria monocytogenes; the combination of hindiii and hpych4iv showed consistently strong signals on gels, amplified an adequate number of dna fragments and detected polymorphism among closely related strains based on asci macrorestriction profiles. aflp also distinguished between l. monocytogenes, l. innocua, l. ivanovii, ...200312866850
prevalence and diversity of tetracycline resistant lactic acid bacteria and their tet genes along the process line of fermented dry sausages.in order to study the prevalence and diversity of tetracycline resistant lactic acid bacteria (tc(r) lab) along the process line of two different fermented dry sausage (fds) types, samples from the raw meat, the meat batter and the fermented end product were analysed quantitatively and qualitatively by using a culture-dependent approach. both the diversity of the tet genes and their bacterial hosts in the different stages of fds production were determined. quantitative analysis showed that all r ...200312866855
inhibition of allergic disorders by infection with bacteria or the exposure to bacterial products.the incidence, severity, and mortality rate of allergic diseases, such as asthma, atopic dermatitis and rhinitis is increasing in the developed nations of the world, despite the use of steroids and other drugs. the reason for this development is unknown, but has been speculated to be due to a steady decline in infectious diseases. this view is supported by recent epidemiological and experimental findings showing that bacterial infections have the capacity to inhibit the development of allergic d ...200312868649
growth kinetics and cell morphology of listeria monocytogenes scott a as affected by temperature, nacl, and edta.growth kinetics and morphological characteristics of listeria monocytogenes scott a grown under stress conditions induced by increasing levels of nacl and edta were studied as a function of temperature. l. monocytogenes scott a was inoculated into brain heart infusion broth (ph 6) at 19, 28, 37, and 42 degrees c. test cultures contained nacl (at concentrations of 4.5, 6.0, and 7.5%) or edta (at concentrations of 0.1, 0.2, and 0.3 mm); control cultures contained 0.5% nacl. growth curves were fitt ...200312870754
a predictive model to determine the effects of temperature, sodium pyrophosphate, and sodium chloride on thermal inactivation of starved listeria monocytogenes in pork slurry.the effects and interactions of 27 combinations of heating temperature (57.5 to 62.5 degrees c), sodium pyrophosphate (spp) level (0 to 0.5%, wt/vol), and salt (nacl) level (0 to 6%, wt/vol) on the thermal inactivation of starved listeria monocytogenes atcc 19116 in pork slurry were investigated. a split-split plot experimental design was used to compare all 27 combinations. l. monocytogenes survivors were enumerated on tryptic soy agar supplemented with 0.6% yeast extract. the natural logarithm ...200312870755
evaluation of antibodies for immunomagnetic separation combined with flow cytometry detection of listeria monocytogenes.four polyclonal anti-listeria antibodies were evaluated for the detection of listeria monocytogenes in direct and indirect assays using immunomagnetic separation with flow cytometry. the efficiency of immunocapturing using magnetic beads was also determined. none of the tested antibodies exhibited sufficient specificity or avidity to allow sufficient separation and detection of l. monocytogenes for a useful test that differentiated between negative (without cell) and positive (with cell) samples ...200312870765
tnf/inos-producing dendritic cells mediate innate immune defense against bacterial infection.dendritic cells (dcs) present microbial antigens to t cells and provide inflammatory signals that modulate t cell differentiation. while the role of dcs in adaptive immunity is well established, their involvement in innate immune defenses is less well defined. we have identified a tnf/inos-producing (tip)-dc subset in spleens of listeria monocytogenes-infected mice that is absent from ccr2-deficient mice. the absence of tip-dcs results in profound tnf and inos deficiencies and an inability to cl ...200312871639
apoptosis facilitates antigen presentation to t lymphocytes through mhc-i and cd1 in tuberculosis.protective immunity against mycobacterium tuberculosis involves major histocompatibility complex class i (mhc-i)- and cd1-restricted cd8 t cells, but the mechanisms underlying antigen delivery to antigen-presenting molecules remain enigmatic. macrophages, the primary host cells for mycobacteria, are cd1-negative. here we show that m. tuberculosis phagosomes are secluded from the cytosolic mhc-i processing pathway and that mycobacteria-infected cells lose their antigen-presenting capacity. we als ...200312872166
listeria monocytogenes peritonitis complicated by septic shock in a patient on continuous ambulatory peritoneal dialysis. 200312872862
macrophages of the splenic marginal zone are essential for trapping of blood-borne particulate antigen but dispensable for induction of specific t cell responses.rapid removal of pathogens from the circulation by secondary lymphoid organs is prerequisite for successful control of infection. blood-borne ags are trapped mainly in the splenic marginal zone. to identify the cell populations responsible for ag trapping in the marginal zone, mice were selectively depleted of marginal zone macrophages and marginal metallophilic macrophages. in the absence of these cells, trapping of microspheres and listeria monocytogenes organisms was lost, and early control o ...200312874200
oral pretreatment of mice with cpg dna reduces susceptibility to oral or intraperitoneal challenge with virulent listeria monocytogenes.listeria monocytogenes is an enteroinvasive intracellular bacterial pathogen that infects humans and other animals, including mice, sometimes resulting in severe systemic infections. previous studies showed that intraperitoneal (i.p.) pretreatment of susceptible balb/c mice with immune-stimulatory cpg dna 48 to 96 h prior to i.p. challenge with virulent l. monocytogenes reduces bacterial numbers in livers by greater than 100-fold, correlating with recovery from infection. here we show that oral ...200312874318
identification of the agr locus of listeria monocytogenes: role in bacterial virulence.listeria monocytogenes is a gram-positive facultative intracellular food-borne pathogen that can cause severe infections in humans and animals. we have recently adapted signature-tagged transposon mutagenesis (stm) to identify genes involved in the virulence of l. monocytogenes. a new round of stm allowed us to identify a new locus encoding a protein homologous to agra, the well-studied response regulator of staphylococcus aureus and part of a two-component system involved in bacterial virulence ...200312874326
influence of the adaptation of listeria monocytogenes populations to structured or homogeneous habitats on subsequent growth on chilled processed meat.the influence of adaptation to habitat structure on the growth of listeria monocytogenes on processed meats was investigated. triplicate populations of each of two l. monocytogenes strains were adapted to growth in homogeneous (tryptic soy broth, tsb) or structured (tryptic soy agar) environments by serial transfer in these media incubated at 25 degrees c for 48 h. adapted (serial transfer for 56 days) and unadapted control (single 48 h growth period) populations were inoculated onto the surface ...200312878388
calcineurin inhibitor-free immunosuppression based on antithymocyte globulin and mycophenolate mofetil in cadaveric kidney transplantation: results after 5 years.kidney grafts from suboptimal donors are more likely to suffer the nephrotoxic side-effects of cyclosporine than kidneys from standard donors. in an attempt to avoid the use of cyclosporine, we carried out a prospective study in low-immunological risk recipients of suboptimal kidneys, using an immunosuppressive protocol combining thymoglobuline in induction with a bi-therapy of mycophenolate mofetil (mmf) and steroids. patients with panel reactive antibodies (pra) <50% receiving a first renal tr ...200312879230
listeriosis in patients at a comprehensive cancer center, 1955-1997.listeria monocytogenes infection occurred in 94 patients during 1955-1997 at memorial sloan-kettering cancer center. the incidence was 0.5 (1955-1966), 0.96 (1970-1979), and 0.14 (1985-1997) cases per 1000 new admissions. eighty-five patients (90%) were bacteremic, and 34 (36%) had evidence of intracranial infection. in 91 patients with cancer, 70 (77%) received chemotherapy for advanced or relapsed malignancy (n=51; 56%); 64 (68%) received corticosteroids. breast cancer was the most common soli ...200312884160
[meningoencephalitis caused by listeria monocytogenes]. 200312886487
[rhomboencephalitis due to listeria monocytogenes as a complication of crohn's disease]. 200312887863
listeria monocytogenes cross-contamination in a nursery [corrected].molecular evidence of listeria monocytogenes cross-contamination in a nursery is presented. listeria monocytogenes serotype 4b was isolated from the blood and the conjunctiva of a baby with neonatal sepsis who was born after septic amnionitis and premature rupture of membrane. nine days later, the same bacterium was isolated from the cerebrospinal fluid of a second baby presenting with meningitis. cervical cultures from the second baby's healthy mother were negative for listeria sp. an in-depth ...200312888770
[clinical reasoning and decision-making in practice. a young woman with fever, shortness of breath, and reduced consciousness].a 23-year-old woman with mild psychomotor retardation presented with fever, coughing, reduced consciousness and a stiff neck. a chest x-ray revealed an infiltrate in the left lower lobe; the cerebrospinal fluid was cloudy with a mild pleocytosis. ceftriaxone was prescribed and the fever subsided. on the second day of admission she had a seizure, and a paraparesis emerged. despite changes in the antibiotic regimen, her clinical condition hardly improved. on the fifth day, antibodies against mycop ...200312894464
detection of listeria monocytogenes in foodstuffs using chromogenic isolation media.a study was carried out to compare two selective plating media commonly used to detect listeria monocytogenes in food samples (palcam and oxford agar) with two more recently formulated chromogenic media (aloa and rapid'l.mono). a comparison was also made between the identification systems api-listeria and monocytogenes id discs. the results obtained from the analysis of 132 food samples showed that the chromogenic media allowed listeria monocytogenes to be detected more rapidly and with higher l ...200312901423
advances in the development of bacterial vector technology.the demand for new and improved vaccines against human diseases has continued unabated over the past century. while the need continues for traditional vaccines in areas such as infectious diseases, there is an increasing demand for new therapies in nontraditional areas, such as cancer treatment, bioterrorism and food safety. prompted by these changes, there has been a renewed interest in the application and development of live, attenuated bacteria expressing foreign antigens as vaccines. the app ...200312901595
altering tissue tropism of listeria monocytogenes by ectopically expressing human e-cadherin in transgenic mice.the gram-positive bacterium listeria monocytogenes invades a variety of cells in vitro and in vivo. here, we show that l. monocytogenes is able to enter muscle cells if provided with the appropriate host receptor. using a cardiac-specific promoter, human e-cadherin was misexpressed in the heart of transgenic mice. first, we demonstrate that l. monocytogenes is capable of infecting the e-cadherin-expressing, but not the n-cadherin-expressing cardiomyocytes in vitro. second, we show that l. monocy ...200312901844
sodium pentobarbital anesthesia transiently enhances the severity of infection following intragastric, but not intravenous, inoculation of listeria monocytogenes in mice.in the present study we observed that mice anesthetized with sodium pentobarbital were far more susceptible to gastrointestinal challenge with listeria monocytogenes than were unanaesthetized mice. the effect of pentobarbital anesthesia was transient (gone within 2 h) and did not alter the severity of infection following i.v. challenge with l. monocytogenes. treatment with pharmacological inhibitors of gastric acidity (i.e. cimetidine and omeprazole), or intestinal motility (loperamide), did not ...200312901847
differences in susceptibility of listeria monocytogenes strains to sakacin p, sakacin a, pediocin pa-1, and nisin.two hundred strains of listeria monocytogenes collected from food and the food industry were analyzed for susceptibility to the class iia bacteriocins sakacin p, sakacin a, and pediocin pa-1 and the class i bacteriocin nisin. the individual 50% inhibitory concentrations (ic(50)) were determined in a microtiter assay and expressed in nanograms per milliliter. the ic(50) of sakacin p ranged from 0.01 to 0.61 ng ml(-1). the corresponding values for pediocin pa-1, sakacin a, and nisin were 0.10 to 7 ...200312902226
biocontrol of listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin.the fresh-cut produce industry has been the fastest-growing portion of the food retail market during the past 10 years, providing consumers with convenient and nutritious food. however, fresh-cut fruits and vegetables raise food safety concerns, because exposed tissue may be colonized more easily by pathogenic bacteria than intact produce. this is due to the higher availability of nutrients on cut surfaces and the greater potential for contamination because of the increased amount of handling. w ...200312902237
curvularia haloperoxidase: antimicrobial activity and potential application as a surface disinfectant.a presumed antimicrobial enzyme system, the curvularia haloperoxidase system, was examined with the aim of evaluating its potential as a sanitizing agent. in the presence of hydrogen peroxide, curvularia haloperoxidase facilitates the oxidation of halides, such as chloride, bromide, and iodide, to antimicrobial compounds. the curvularia haloperoxidase system caused several-log-unit reductions in counts of bacteria (pseudomonas spp., escherichia coli, serratia marcescens, aeromonas salmonicida, s ...200312902249
evaluation of the use of pcr and reverse transcriptase pcr for detection of pathogenic bacteria in biosolids from anaerobic digestors and aerobic composters.a pcr-based method and a reverse transcriptase pcr (rt-pcr)-based method were developed for the detection of pathogenic bacteria in organic waste, using salmonella spp., listeria monocytogenes, yersinia enterocolitica, and staphylococcus aureus as model organisms. in seeded organic waste samples, detection limits of less than 10 cells per g of organic waste were achieved after one-step enrichment of bacteria, isolation, and purification of dna or rna before pcr or rt-pcr amplification. to test t ...200312902250
listeria monocytogenes occurrence and characterization in meat-producing plants.the prevalence, level of contamination and epidemiological profile of listeria monocytogenes were investigated in two meat-producing plants during a 20-month period.200312904225
screening of bifidobacterium strains isolated from human faeces for antagonistic activities against potentially bacterial pathogens.as probiotic bacteria, strains belonging to the genus bifidobacterium colonise the gastro-intestinal tract of humans and animals at the time of birth, and they are found in young as well as in adult individuals in great numbers. moreover, they can interact with the development of enteric infections by the production of antimicrobial metabolites. in this work 281 strains of bifidobacteria were anaerobically isolated from human faecal samples, supplied by volunteers of different ages (youngs, adul ...200312906392
[meningitis due to listeria monocytogenes as a complication of infliximab therapy].a 41-year-old woman was admitted to the hospital with meningitis caused by listeria monocytogenes. because of her crohn's disease she used prednisolone and azathioprine. two weeks before presenting with meningitis, infliximab had been given as the other immunosuppressant drugs had no effect. this tumour necrosis factor alpha (tnf alpha) blocking agent is known to increase the risk of opportunistic infections. this is the first dutch patient described with meningitis caused by l. monocytogenes af ...200312908351
evaluation of the ph-dependent, stationary-phase acid tolerance in listeria monocytogenes and salmonella typhimurium dt104 induced by culturing in media with 1% glucose: a comparative study with escherichia coli o157:h7.to comparatively evaluate the adaptive stationary-phase acid tolerance response (atr) in food-borne pathogens induced by culturing in glucose-containing media, as affected by strain variability and antibiotic resistance, growth temperature, challenge ph and type of acidulant.200312911705
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