Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| systematic review of the in vitro effects of statins on oral and perioral microorganisms. | statins are medications administered orally and are widely used for lowering the blood cholesterol level. the aim of this study was to investigate the effects of orally administered statins on microorganisms infecting oral and perioral tissues. we performed a systematic review of published studies of the in vitro antimicrobial effects of statins on bacteria, viruses, and fungi, and searched pubmed, web of science, cochrane central, and google scholar. studies show that most statins exhibit antim ... | 2016 | 26718458 |
| deletion of afpab1 causes increased sensitivity to oxidative stress and hypovirulence in aspergillus fumigatus. | aspergillus fumigatus afpab1 is the ortholog of the aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules aopab1 that function to depress the initiation of translation during stress. a. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research on pab1 in a. fumigatus. the associated gene afpab1 was replaced with a hygromycin-selectable marker to generate the strain δafpab1. phenotypic analysis showed that the δafpab1 grew m ... | 2016 | 27801871 |
| identification and characterization of an anti-oxidative stress-associated mutant of aspergillus fumigatus transformed by agrobacterium tumefaciens. | aspergillus fumigatus is one of the most common opportunistic pathogenic fungi, surviving in various environmental conditions. maintenance of the redox homeostasis of the fungus relies upon the well‑organized regulation between reactive oxygen species generated by immune cells or its own organelles, and the activated anti‑oxidative stress mechanism. to investigate such a mechanism, the present study obtained a number of randomly‑inserted mutants of a. fumigatus, mediated by agrobacterium tumefac ... | 2016 | 26847000 |
| [cross-reactivity in allergic fungal sinusitis. case report]. | the presence of allergic mucin in allergic fungal sinusitis (afs) is a manifestation that identifies it as a hypersensitivity process. afs has a phenomenon of cross-reactivity to ige-bound proteins having at least two shared epitopes. | 2016 | 27560920 |
| clinical distinctness of allergic rhinitis in patients with allergy to molds. | introduction. molds are a very diverse group of allergens. exposure and sensitization to fungal allergens can promote the development and worsening of allergic rhinitis (ar). objective. the natural course of allergic rhinitis was compared between a group of patients with allergy to molds and patients with ar to other allergens as the control groups. material and methods. the study group consisted of 229 patients, with a mean age of 27.4 ± 6.5 yrs. the study group was compared to groups of ar pat ... | 2016 | 27340656 |
| [prevalence of sensitization to fungi in patients with respiratory allergy]. | as part of the etiology of respiratory allergy we have genetics, prenatal factors and sensitivity to various airborne allergens, between these fungi are found. relationship has been found between sensitization to fungal in skin tests and allergy pathogenesis and aggravation. there is a few literature in mexico and in the north of the country it is lacking regarding this problem. | 2016 | 27174757 |
| effect of plant extracts on book deteriorated fungal species. | the aim of the study was to evaluate the effect of leaf extracts of four plants against some isolated fungal species from deteriorated books. aqueous, methanol and chloroform extracts of selected plant species were screened in vitro for their antifungal activity against some book deteriorating fungal species. fifteen species belonging to 09 genera were isolated and identified from infested books in library. aqueous and solvent extracts of leaves of azadiracta indica, callistemon citrinus, eucaly ... | 2016 | 27154063 |
| how to diagnose mould allergy? comparison of skin prick tests with specific ige results. | diagnosis of mould allergy is complicated due to the heterogeneity of the test material and the decrease in the number of commercial mould skin test solutions that are currently available. | 2016 | 27027397 |
| the importance of mold sensitivity in nonallergic rhinitis patients. | in recent studies, local specific immunoglobulin e (sige) production against allergens in nasal mucosa and a positive response to a nasal allergen provocation test (napt) have been demonstrated in some patients initially diagnosed as nonallergic rhinitis (nar) or idiopathic rhinitis (ir). the aim of this study was to evaluate the presence of local allergic rhinitis (lar) in patients who experience rhinitis symptoms in indoor and outdoor moldy conditions and to investigate the role of the napt in ... | 2016 | 26880361 |
| evaluation of antifungal efficacy of 0.1% and 0.25% riboflavin with uva: a comparative in vitro study. | antifungal efficacy of photochemical cross-linking (pack-cxl) with 0.1% and 0.25% riboflavin was evaluated with a comparative in vitro study. | 2016 | 26644282 |
| biochemical characterization, thermal stability, and partial sequence of a novel exo-polygalacturonase from the thermophilic fungus rhizomucor pusillus a13.36 obtained by submerged cultivation. | this work reports the production of an exo-polygalacturonase (exo-pg) by rhizomucor pusillus a13.36 in submerged cultivation (smc) in a shaker at 45°c for 96 h. a single pectinase was found and purified in order to analyze its thermal stability, by salt precipitation and hydrophobic interaction chromatography. the pectinase has an estimated mw of approximately 43.5-47 kda and optimum ph of 4.0 but is stable in ph ranging from 3.5 to 9.5 and has an optimum temperature of 61°c. it presents thermal ... | 2016 | 28025649 |
| effect of sticky mat usage in control of nosocomial infection in motahary burn hospital. | infection is the most common cause of death among burnt patients and infection control decrease the rate of mortality. the use of sticky mat can control contamination by preventing the entrance microorganisms into the hospital wards. this study was designed to evaluate the sticky mats effect in reduction of microorganism's entry by personnel shoes to burn intensive care unit (bicu). | 2016 | 27928489 |
| vermamoeba vermiformis-aspergillus fumigatus relationships and comparison with other phagocytic cells. | free living amoebae (fla) are protists ubiquitously present in the environment. aspergillus fumigatus is a mould responsible for severe deep-seated infections, and that can be recovered in the same habitats as the fla. by conducting coculture experiments and fungal incubation with amoebal supernatants, we report herein that vermamoeba vermiformis, a fla present in hospital water systems, promotes filamentation and growth of a. fumigatus. this finding is of particular importance to institutions w ... | 2016 | 27381330 |
| retrospective analysis of the microbiological spectrum of pneumonia in turkish patients with lung cancer. | the spectrum of pulmonary infections in patients with lung cancer is wide, and tools for target-oriented infection control measures are necessary. in this retrospective study we report the microbiological spectrum of pneumonia (based on the results of sputum culture) in a case series of turkish patients with lung malignancies. | 2016 | 27095942 |
| deacetylation of fungal exopolysaccharide mediates adhesion and biofilm formation. | the mold aspergillus fumigatus causes invasive infection in immunocompromised patients. recently, galactosaminogalactan (gag), an exopolysaccharide composed of galactose and n-acetylgalactosamine (galnac), was identified as a virulence factor required for biofilm formation. the molecular mechanisms underlying gag biosynthesis and gag-mediated biofilm formation were unknown. we identified a cluster of five coregulated genes that were dysregulated in gag-deficient mutants and whose gene products s ... | 2016 | 27048799 |
| development and evaluation of a loop-mediated isothermal amplification method for rapid detection of aspergillus fumigatus. | aspergillus fumigatusis a conditional pathogen and the major cause of life-threatening invasive aspergillosis (ia) in immunocompromised patients. the early and rapid detection ofa. fumigatusinfection is still a major challenge. in this study, the new member of the fungal annexin family, annexin c4, was chosen as the target to design a loop-mediated isothermal amplification (lamp) assay for the rapid, specific, and sensitive detection ofa. fumigatus the evaluation of the specificity of the lamp a ... | 2016 | 26791368 |
| volatile compounds emitted by pseudomonas aeruginosa stimulate growth of the fungal pathogen aspergillus fumigatus. | chronic lung infections with opportunistic bacterial and fungal pathogens are a major cause of morbidity and mortality especially in patients with cystic fibrosis. pseudomonas aeruginosa is the most frequently colonizing bacterium in these patients, and it is often found in association with the filamentous fungus aspergillus fumigatus. p. aeruginosa is known to inhibit the growth of a. fumigatus in situations of direct contact, suggesting the existence of interspecies communication that may infl ... | 2016 | 26980832 |
| evaluation of microbiological and chemical contaminants in poultry farms. | the aim of the study was to evaluate the microbiological and chemical contamination in settled dust at poultry farms. the scope of research included evaluating the contributions of the various granulometric fractions in settled dust samples, assessing microbial contamination using culture methods, concentrations of secondary metabolites in dust and their cytotoxicity against hepatocyte chicken cells by means of mtt (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) tests. in addition, ... | 2016 | 26861361 |
| phylogenetic and structural analysis of polyketide synthases in aspergilli. | polyketide synthases (pkss) of aspergillus species are multidomain and multifunctional megaenzymes that play an important role in the synthesis of diverse polyketide compounds. putative pks protein sequences from aspergillus species representing medically, agriculturally, and industrially important aspergillus species were chosen and screened for in silico studies. six candidate aspergillus species, aspergillus fumigatus af293, aspergillus flavus nrrl3357, aspergillus niger cbs 513.88, aspergill ... | 2016 | 27199544 |
| interactions of aspergillus fumigatus conidia with airway epithelial cells: a critical review. | aspergillus fumigatus is an environmental filamentous fungus that also acts as an opportunistic pathogen able to cause a variety of symptoms, from an allergic response to a life-threatening disseminated fungal infection. the infectious agents are inhaled conidia whose first point of contact is most likely to be an airway epithelial cell (aec). the interaction between epithelial cells and conidia is multifaceted and complex, and has implications for later steps in pathogenesis. increasing evidenc ... | 2016 | 27092126 |
| flow cytometry of lung and bronchoalveolar lavage fluid cells from mice challenged with fluorescent aspergillus reporter (flare) conidia. | aspergillus fumigatus is a ubiquitous fungal pathogen that forms airborne conidia. the process of restricting conidial germination into hyphae by lung leukocytes is critical in determining infectious outcomes. tracking the outcome of conidia-host cell encounters in vivo is technically challenging and an obstacle to understanding the molecular and cellular basis of antifungal immunity in the lung. here, we describe a method that utilizes a genetically engineered aspergillus strain [called flare ( ... | 2016 | 28691040 |
| osteomyelitis and lung abscess due to aspergillus fumigatus in a chronic granulomatous disease patient. | chronic granulomatous disease (cgd) is an inherited disorder of the nicotinamide adenine dinucleotide phosphate (nadph) oxidase complex. this disorder results in recurrent life-threatening bacterial and fungal infections. aspergillus species are the most common fungal infections in these patients. | 2016 | 28681028 |
| suspected allergic bronchopulmonary aspergillosis cases in adult bronchial asthma patients attending a tertiary care clinic. | allergic bronchopulmonary aspergillosis (abpa) is a hypersensitivity reaction to aspergillus species (generally aspergillus fumigatus) that occurs almost exclusively in patients with asthma or, less commonly, cystic fibrosis. immune responses to aspergillus antigens cause airway obstruction and, if untreated, bronchiectasis and pulmonary fibrosis. our objective was to define the clinical characteristics, laboratory and radiological findings of suspected abpa cases among a cohort of egyptian pati ... | 2016 | 28502150 |
| microbial communication leading to the activation of silent fungal secondary metabolite gene clusters. | microorganisms form diverse multispecies communities in various ecosystems. the high abundance of fungal and bacterial species in these consortia results in specific communication between the microorganisms. a key role in this communication is played by secondary metabolites (sms), which are also called natural products. recently, it was shown that interspecies "talk" between microorganisms represents a physiological trigger to activate silent gene clusters leading to the formation of novel sms ... | 2015 | 25941517 |
| the zinc transport systems and their regulation in pathogenic fungi. | zinc is an essential micronutrient required for many enzymes that play essential roles in a cell. it was estimated that approximately 3% of the total cellular proteins are required for zinc for their functions. zinc has long been considered as one of the key players in host-pathogen interactions. the host sequesters intracellular zinc by utilizing multiple cellular zinc importers and exporters as a means of nutritional immunity. to overcome extreme zinc limitation within the host environment, pa ... | 2015 | 26539032 |
| pseudomonas aeruginosa inhibits the growth of scedosporium aurantiacum, an opportunistic fungal pathogen isolated from the lungs of cystic fibrosis patients. | the filamentous fungus scedosporium aurantiacum and the bacterium pseudomonas aeruginosa are opportunistic pathogens isolated from lungs of the cystic fibrosis (cf) patients. p. aeruginosa has been known to suppress the growth of a number of cf related fungi such as aspergillus fumigatus, candida albicans, and cryptococcus neoformans. however, the interactions between p. aeruginosa and s. aurantiacum have not been investigated in depth. hence we assessed the effect of p. aeruginosa reference str ... | 2015 | 26379643 |
| sph3 is a glycoside hydrolase required for the biosynthesis of galactosaminogalactan in aspergillus fumigatus. | aspergillus fumigatus is the most virulent species within the aspergillus genus and causes invasive infections with high mortality rates. the exopolysaccharide galactosaminogalactan (gag) contributes to the virulence of a. fumigatus. a co-regulated five-gene cluster has been identified and proposed to encode the proteins required for gag biosynthesis. one of these genes, sph3, is predicted to encode a protein belonging to the spherulin 4 family, a protein family with no known function. construct ... | 2015 | 26342082 |
| validation of a new aspergillus real-time pcr assay for direct detection of aspergillus and azole resistance of aspergillus fumigatus on bronchoalveolar lavage fluid. | azole resistance in aspergillus fumigatus is increasingly reported. here, we describe the validation of the aspergenius, a new multiplex real-time pcr assay consisting of two multiplex real-time pcrs, one that identifies the clinically relevant aspergillus species, and one that detects the tr34, l98h, t289a, and y121f mutations in cyp51a and differentiates susceptible from resistant a. fumigatus strains. the diagnostic performance of the aspergenius assay was tested on 37 bronchoalveolar lavage ... | 2015 | 25568431 |
| characterization of the fkbp12-encoding genes in aspergillus fumigatus. | invasive aspergillosis, largely caused by aspergillus fumigatus, is responsible for a growing number of deaths among immunosuppressed patients. immunosuppressants such as fk506 (tacrolimus) that target calcineurin have shown promise for antifungal drug development. fk506-binding proteins (fkbps) form a complex with calcineurin in the presence of fk506 (fkbp12-fk506) and inhibit calcineurin activity. research on fkbps in fungi is limited, and none of the fkbps have been previously characterized i ... | 2015 | 26366742 |
| molecular characterization of lc3-associated phagocytosis reveals distinct roles for rubicon, nox2 and autophagy proteins. | lc3-associated phagocytosis (lap) is a process wherein elements of autophagy conjugate lc3 to phagosomal membranes. we characterize the molecular requirements for lap, and identify rubicon as being required for lap but not autophagy. rubicon is recruited to laposomes and is required for the activity of a class iii pi(3)k complex containing uvrag but lacking atg14 and ambra1. this allows for the sustained localization of ptdins(3)p, which is critical for recruitment of downstream autophagic prote ... | 2015 | 26098576 |
| il-1α signaling is critical for leukocyte recruitment after pulmonary aspergillus fumigatus challenge. | aspergillus fumigatus is a mold that causes severe pulmonary infections. our knowledge of how a. fumigatus growth is controlled in the respiratory tract is developing, but still limited. alveolar macrophages, lung resident macrophages, and airway epithelial cells constitute the first lines of defense against inhaled a. fumigatus conidia. subsequently, neutrophils and inflammatory ccr2+ monocytes are recruited to the respiratory tract to prevent fungal growth. however, the mechanism of neutrophil ... | 2015 | 25629406 |
| compartment-specific and sequential role of myd88 and card9 in chemokine induction and innate defense during respiratory fungal infection. | aspergillus fumigatus forms ubiquitous airborne conidia that humans inhale on a daily basis. although respiratory fungal infection activates the adaptor proteins card9 and myd88 via c-type lectin, toll-like, and interleukin-1 family receptor signals, defining the temporal and spatial pattern of myd88- and card9-coupled signals in immune activation and fungal clearance has been difficult to achieve. herein, we demonstrate that myd88 and card9 act in two discrete phases and in two cellular compart ... | 2015 | 25621893 |
| amination of ω-functionalized aliphatic primary alcohols by a biocatalytic oxidation-transamination cascade. | amination of non-activated aliphatic fatty alcohols to the corresponding primary amines was achieved through a five-enzyme cascade reaction by coupling a long-chain alcohol oxidase from aspergillus fumigatus (lcao_af) with a ω-transaminase from chromobacterium violaceum (ω-ta_cv). the alcohol was oxidized at the expense of molecular oxygen to yield the corresponding aldehyde, which was subsequently aminated by the plp-dependent ω-ta to yield the final primary amine product. the overall cascade w ... | 2015 | 26583050 |
| effect of treatment with an overheated dry-saturated steam vapour disinfection system on multidrug and extensively drug-resistant nosocomial pathogens and comparison with sodium hypochlorite activity. | the development of portable steam generators has made disinfection of the environment more practical. this study assessed the "in vitro" ability of an overheated dry-saturated steam vapour system to kill multidrug and extensively-drug resistant nosocomial pathogens, defining the antimicrobial spectrum and the contact times compared with the activity of sodium hypochlorite. | 2015 | 26452549 |
| long-term pulmonary infections in heart transplant recipients. | pulmonary infections are life-threatening complications in heart transplant recipients. our aim was to evaluate long-term pulmonary infections and the effect of prophylactic antimicrobial strategies on time of occurrence of pulmonary infections in heart transplant recipients. | 2015 | 25894190 |
| antifungal and antibacterial metabolites from a french poplar type propolis. | during this study, the in vitro antifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a french propolis batch were evaluated. antifungal activity was evaluated by broth microdilution on three pathogenic strains: candida albicans, c. glabrata, and aspergillus fumigatus. antibacterial activity was assayed using agar dilution method on 36 gram-negative and gram-positive strains including staphylococcus aureus. organic extracts showed a significant antifun ... | 2015 | 25873978 |
| aspergillus galactomannan detection in comparison to a real-time pcr assay in serum samples from a high-risk group of patients. | invasive aspergillosis (ia) is a severe infection with a 70% mortality rate. aspergillus fumigatus is responsible for over 90% of those infections. the diagnosis of invasive aspergillosis is based on clinical sample culture and detection of fungal hyphae in histopathological examination. additional tests may include the detection of the galactomannan antigen and of fungal genetic material in serum and bronchoalveolar washings. the present study was to assess the use of these two rapid tests in t ... | 2015 | 26862310 |
| mortality associated with avian reovirus infection in a free-living magpie (pica pica) in great britain. | avian reoviruses (arvs) cause a range of disease presentations in domestic, captive and free-living bird species. arvs have been reported as a cause of significant disease and mortality in free-living corvid species in north america and continental europe. until this report, there have been no confirmed cases of arv-associated disease in british wild birds. | 2015 | 25880683 |
| the role of fungal sensitisation in clinical presentation in patients with chronic obstructive pulmonary disease. | atopic patients with chronic obstructive pulmonary disease (copd) demonstrate more severe symptoms than their non-atopic counterparts. also, aspergillus hypersensitivity is known in copd. however, allergic sensitisation to non-aspergillus fungi has never been studied in copd patients. to evaluate the prevalence of fungal sensitisation and its impact on the clinical presentation and outcome of copd patients. sensitisation to 17 fungi was studied in 55 copd patients through skin prick tests, fungu ... | 2015 | 26201384 |
| variable risk of atopic disease due to indoor fungal exposure in nhanes 2005-2006. | exposure to damp indoor environments is associated with increased risk of eczema, allergy and asthma. the role of dampness-related exposures and risk of allergic diseases are yet to be fully explored in the us population. | 2015 | 25845975 |
| superoxide dismutase reduces the inflammatory response to aspergillus and alternaria in human sinonasal epithelial cells derived from patients with chronic rhinosinusitis. | aspergillus fumigatus and alternaria alternata are ubiquitous environmental fungal allergens that can exacerbate airway inflammation and contribute to the disease process in patients with chronic rhinosinusitis (crs). these antigens have been shown to induce human sinonasal epithelial cells (hsnecs) to promote a proinflammatory response, but what is unclear is a means by which to reduce these effects. inhaled pathogens can induce hsnecs to produce reactive oxygen species (ros) that trigger cytok ... | 2015 | 25785747 |
| pediatric severe asthma with fungal sensitization is mediated by steroid-resistant il-33. | the mechanism underlying severe asthma with fungal sensitization (safs) is unknown. il-33 is important in fungus-induced asthma exacerbations, but its role in fungal sensitization is unexplored. | 2015 | 25746970 |
| characterisation of alternaria alternata manganese-dependent superoxide dismutase, a cross-reactive allergen homologue to asp f 6. | it is well known that alternaria alternata presents a significant level of allergenic cross-reactivity with several other phylogenetically related and non-related allergenic moulds. to improve the molecular diagnosis, the identification and characterisation of all clinically relevant allergens, including both species-specific and cross-reacting proteins, is required. in this study we report the molecular and immunological characterisation of the a. alternata manganese-dependent superoxide dismut ... | 2015 | 25657116 |
| allergen sensitization profiles in a population-based cohort of children hospitalized for asthma. | allergen sensitization is associated with asthma morbidity. a better understanding of allergen sensitization patterns among children hospitalized for asthma could help clinicians tailor care more effectively. to our knowledge, however, sensitization profiles among children hospitalized for asthma are unknown. | 2015 | 25594255 |
| pcr-rflp on β-tubulin gene for rapid identification of the most clinically important species of aspergillus. | aspergillus species are important agents of life-threatening infections in immunosuppressed patients. proper speciation in the aspergilli has been justified based on varied fungal virulence, clinical presentations, and antifungal resistance. accurate identification of aspergillus species usually relies on fungal dna sequencing but this requires expensive equipment that is not available in most clinical laboratories. we developed and validated a discriminative low-cost pcr-based test to discrimin ... | 2015 | 26264625 |
| cellular cytokine and chemokine responses to parasite antigens and fungus and mite allergens in children co-infected with helminthes and protozoa parasites. | in sub-saharan africa poly-parasite infections are frequently observed in children, and with poly-parasitism modulating immune mechanisms, mediated by cytokines and chemokines, are required to prevent overwhelming inflammation and host tissue damage. we analyzed in children co-infected with helminthes and protozoan parasites their cellular production of regulatory and pro-inflammatory cytokines and chemokines in response to parasite antigens and allergens. | 2015 | 25698903 |
| identification and characterization of an antifungal protein, afafpr9, produced by marine-derived aspergillus fumigatus r9. | a fungal strain, r9, was isolated from the south atlantic sediment sample and identified as aspergillus fumigatus. an antifungal protein, afafpr9, was purified from the culture supernatant of aspergillus fumigatus r9. afafpr9 was identified to be restrictocin, which is a member of the ribosome-inactivating proteins (rips), by maldi-tof-tof-ms. afafpr9 displayed antifungal activity against plant pathogenic fusarium oxysporum, alternaria longipes, colletotrichum gloeosporioides, paecilomyces vario ... | 2015 | 25394604 |
| antiproliferative effect and characterization of a novel antifungal peptide derived from human chromogranin a. | cga-n46 is a novel antifungal peptide derived from the n-terminus of human chromogranin a, corresponding to the 31st to 76th amino acids. further research on its activities and characteristics may be helpful for the application of cga-n46 in medical or other situations. in the present study, the antifungal spectrum and physicochemical characteristics of cga-n46 were investigated using an antifungal assay, its antiproliferative effects on cancer and normal cells were assessed using mtt assay and ... | 2015 | 26668630 |
| evaluation of nested pcr in diagnosis of fungal rhinosinusitis. | given the importance of rapid diagnosis for fungal rhinosinusitis, this study aimed to evaluate the use of nested pcr to identify aspergillus and mucor species in clinical samples from patients with suspected fungal rhinosinusitis. | 2015 | 26644876 |
| fungal infections after boston type 1 keratoprosthesis implantation: literature review and in vitro antifungal activity of hypochlorous acid. | to review the current literature describing cases of fungal keratitis and endophthalmitis after boston keratoprosthesis (kpro) implantation and to characterize the antifungal activity of 0.01% hypochlorous acid against medically relevant fungi. | 2015 | 26488624 |
| hgt-finder: a new tool for horizontal gene transfer finding and application to aspergillus genomes. | horizontal gene transfer (hgt) is a fast-track mechanism that allows genetically unrelated organisms to exchange genes for rapid environmental adaptation. we developed a new phyletic distribution-based software, hgt-finder, which implements a novel bioinformatics algorithm to calculate a horizontal transfer index and a probability value for each query gene. applying this new tool to the aspergillus fumigatus, aspergillus flavus, and aspergillus nidulans genomes, we found 273, 542, and 715 transf ... | 2015 | 26473921 |
| sonic hedgehog-responsive lipoxygenases and cyclooxygenase-2 modulate dectin-1-induced inflammatory cytokines. | immune responses during fungal infections are predominately mediated by 5/15-lipoxygenases (lo)- or cyclooxygenase (cox)-2-catalysed bioactive eicosanoid metabolites like leukotrienes, lipoxins and prostaglandins. although few host mediators of fungi-triggered eicosanoid production have been established, the molecular mechanism of expression and regulation of 5-lo, 15-lo and cox-2 are not well-defined. here, we demonstrate that, macrophages infected with representative fungi candida albicans, as ... | 2015 | 26432261 |
| assessment of susceptibility of mould isolates towards biocides. | the aim of this study was to determine the susceptibility of 77 mould strains: aspergillus fumigatus (20), aspergillus flavus (8), aspergillus niger (4), aspergillus ochraceus (2), penicillium citrinum (15), penicillium crysogenum (14), penicillium aurantiogriseum (1), penicillium roquefortii (4), penicillium paneum (2), rhizopus spp. (3), tricoderma spp. (1) and mucor spp. (3) to biocides. | 2015 | 26420602 |
| chronological aging in conidia of pathogenic aspergillus: comparison between species. | aspergillus fumigatus, aspergillus flavus, aspergillus terreus and aspergillus niger are common airborne fungi, and the most frequent causative agents of human fungal infections. however, the resistance and lifetime persistence of these fungi in the atmosphere, and the mechanism of aging of aspergillus conidia are unknown.with this work, we intended to study the processes underlying conidial aging of these four relevant and pathogenic aspergillus species. chronological aging was therefore evalua ... | 2015 | 26341609 |
| in vitro interactions of antifungal agents and tacrolimus against aspergillus biofilms. | aspergillus biofilms were prepared from aspergillus fumigatus, aspergillus flavus, and aspergillus terreus via a 96-well plate-based method, and the combined antifungal activity of tacrolimus with azoles or amphotericin b against aspergillus biofilms was investigated via a broth microdilution checkerboard technique system. our results suggest that combinations of tacrolimus with voriconazole or amphotericin b have synergistic inhibitory activity against aspergillus biofilms. however, combination ... | 2015 | 26303797 |
| biological activities of two macroalgae from adriatic coast of montenegro. | in the present investigation the acetone extracts of macroalgae ulva lactuca and enteromorpha intestinalis were tested for antioxidant, antimicrobial and cytotoxic potential. antioxidant activity was evaluated by measuring the scavenging capacity of tested samples on dpph and superoxide anion radicals, reducing the power of samples and determination of total phenolic and flavonoid compounds in extracts. as a result of the study, u. lactuca extract was found to have a better free radical scavengi ... | 2015 | 26150743 |
| cutaneous invasive aspergillosis: retrospective multicenter study of the french invasive-aspergillosis registry and literature review. | invasive aspergillosis (ia) has poor prognosis in immunocompromised patients. skin manifestations, when present, should contribute to an early diagnosis. the authors aimed to provide prevalence data and a clinical and histologic description of cutaneous manifestations of primary cutaneous ia (pcia) and secondary cia (scia) in a unique clinical series of ia and present the results of an exhaustive literature review of cia. cases of proven and probable ia with cutaneous manifestations were retrosp ... | 2015 | 26131805 |
| sertraline enhances the activity of antimicrobial agents against pathogens of clinical relevance. | serotonin reuptake inhibitors were recently reported to possess antimicrobial potentials, potentiate activity of several antibiotics, reverse multidrug resistant phenotypes of bacteria and make them susceptible to previously resistant drugs. we investigated antimicrobial potentials of sertraline (sr) against atcc strains, clinical isolates of staphylococcus aureus, escherichia coli and pseudomonas aeruginosa alone and in-combination with seven antibiotics. antifungal activity was investigated ag ... | 2015 | 26029671 |
| material properties and antimicrobial activity of polyhydroxybutyrate (phb) films incorporated with vanillin. | polyhydroxybutyrate (phb) was produced by bacillus mycoides dfc 1, isolated from garden soil. antimicrobial (am) films of phb were prepared by incorporating vanillin (4-hydroxy-3-methoxybenzaldehyde) from 10 to 200 μg/g of phb. the films were assessed for antimicrobial activity against foodborne pathogens and spoilage bacteria comprising of escherichia coli, salmonella typhimurium, shigella flexneri, and staphylococcus aureus and fungi such as aspergillus flavus, aspergillus fumigatus, aspergill ... | 2015 | 26002697 |
| characterization of the bioactive metabolites from a plant growth-promoting rhizobacteria and their exploitation as antimicrobial and plant growth-promoting agents. | a plant growth-promoting bacterial strain, pm 105, isolated from a tea plantation soil from the north eastern region of india was identified as pseudomonas aeruginosa through classical and 16s ribosomal dna (rdna) gene sequencing. further studies with this strain confirmed broad spectrum antifungal activity against ten human and plant pathogenic fungal pathogens viz. aspergillus flavus, aspergillus fumigatus, aspergillus niger, aspergillus tubingensis, candida albicans, colletotrichum gloeospori ... | 2015 | 25832181 |
| fungal burden in waste industry: an occupational risk to be solved. | high loads of fungi have been reported in different types of waste management plants. this study intends to assess fungal contamination in one waste-sorting plant before and after cleaning procedures in order to analyze their effectiveness. air samples of 50 l were collected through an impaction method, while surface samples, taken at the same time, were collected by the swabbing method and subject to further macro- and microscopic observations. in addition, we collected air samples of 250 l usi ... | 2015 | 25796518 |
| detection of aspergillus flavus and a. fumigatus in bronchoalveolar lavage specimens of hematopoietic stem cell transplants and hematological malignancies patients by real-time polymerase chain reaction, nested pcr and mycological assays. | pulmonary aspergillosis (pa) is one of the most serious complications in immunocompromised patients, in particular among hematopoietic stem cell transplants (hsct) and patients with hematological malignancies. | 2015 | 25763133 |
| adrenal and hepatic aspergillosis in an immunocompetent patient. | invasive aspergillosis, an infection most frequently induced by aspergillus fumigatus and aspergillus flavus, typically occurs in immunocompromised patients and is usually transmitted through inhalation of aspergillus spores. as the lungs are by far the most common site involved in invasive aspergillosis and invasive aspergillosis in immunocompetent hosts is very rare, there have been a few case reports of extra-pulmonary, disseminated invasive aspergillosis in immunocompetent persons. herein, w ... | 2015 | 25692352 |
| detection of aspergillus-specific antibodies by agar gel double immunodiffusion and igg elisa in feline upper respiratory tract aspergillosis. | feline upper respiratory tract aspergillosis (urta) is an emerging infectious disease. the aims of this study were: (1) to assess the diagnostic value of detection of aspergillus-specific antibodies using an agar gel double immunodiffusion (agid) assay and an indirect immunoglobulin g (igg) elisa; and (2) to determine if an aspergillin derived from mycelia of aspergillus fumigatus, aspergillus niger and aspergillus flavus can be used to detect serum antibodies against cryptic aspergillus spp. in ... | 2015 | 25634077 |
| maldi-tof mass spectrometry for rapid identification of clinical fungal isolates based on ribosomal protein biomarkers. | this study aimed to evaluate the identification of clinical fungal isolates (yeast and molds) by protein profiling using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof/ms). a total of 125 clinical fungal culture isolates (yeast and filamentous fungi) were collected. the test set included 88 yeast isolates (candida albicans, candida glabrata, candida guilliermondii, candida kefyr, candida krusei, candida parapsilosis, candida rugosa, candida tropicalis and ... | 2015 | 25541362 |
| cutaneous, respiratory and hepatic aspergillosis in brazilian white pekin mallards (anas platyrhynchos). | aspergillosis is one of the most frequent mycosis affecting avian species. here is reported an outbreak of aspergillosis affecting 60-day-old white pekin mallards (anas platyrhynchos). about 10% of animals in a lot of 200 mallards from a commercial husbandry presented respiratory disorders and skin lesions at slaughter. three out of 13 animals sent to diagnosis showed, simultaneously, airsacculitis, lung and liver presenting white nodules with variable diameters and elevated, yellowish brown, cr ... | 2015 | 25481845 |
| invasive aspergillosis in liver transplant recipients: epidemiology, clinical characteristics, treatment, and outcomes in 116 cases. | invasive aspergillosis (ia) in liver transplant recipients is associated with grave outcomes. we reviewed 116 individual cases reported in the literature from 1985 to 2013. ia was diagnosed after a median of 25 days after transplantation and involved a single organ in 51% of the cases, whereas in the remaining cases, multiple sites were involved. the most common infecting aspergillus species were aspergillus fumigatus (73%), aspergillus flavus (14%), and aspergillus terreus (8%). amphotericin b ... | 2015 | 25348192 |
| unusual causes of fungal rhinosinusitis: a study from a tertiary care centre in south india. | the frequency of mycotic infections of the nose and paranasal sinuses has been increasing over the past three decades. apart from the common causes of fungal rhinosinusitis such as aspergillus species and penicillium species, there have been reports of rare and unusual fungi isolated from india and other countries. | 2015 | 24064646 |
| 1,1-diphenyl,2-picrylhydrazyl free radical scavenging, bactericidal, fungicidal and leishmanicidal properties of teucrium stocksianum. | in the present study, we evaluated the pharmacological and toxicological effects of teucrium stocksianum. the crude extract of t. stocksianum (ts.cr) and its subsequent organic fractions: n-hexane (ts.hex), chloroform (ts.chcl3) and ethyl acetate (ts.etac) exhibited 1,1-diphenyl,2-picrylhydrazyl free radical scavenging activity with different potencies. ts.etac was found to be most potent. ts.cr, ts.hex, ts.chcl3 and ts.etac showed significant bactericidal activity against escherichia coli, stap ... | 2015 | 23625908 |
| screening of xylanolytic aspergillus fumigatus for prebiotic xylooligosaccharide production using bagasse. | sugarcane bagasse is an important lignocellulosic material studied for the production of xylooligosaccharides (xos). some xos are considered soluble dietary fibre, with low caloric value and prebiotic effect, but they are expensive and not easily available. in a screening of 138 fungi, only nine were shortlisted, and just aspergillus fumigatus m51 (35.6 u/ml) and a. fumigatus u2370 (28.5 u/ml) were selected as the most significant producers of xylanases. these fungi had low β-xylosidase activity ... | 2015 | 27904377 |
| reviewing the importance and evolution of fungal infections and potential antifungal resistance in haematological patients. | there is a growing body of evidence supporting the emergence of azole resistance in aspergillus and candida spp. and this may be of particular concern due to the potential implications in the management of invasive fungal infections occurring in immunocompromised hosts. the aim of present review was to describe the magnitude of the problem, summarising the epidemiology and potential impact of yeast and mould antifungal resistance in patients with haematological malignancies. the first cases of t ... | 2015 | 27842866 |
| intracranial aspergillus fumigatus infection complicated with cavernous hemangioma: case report and literature review. | the aim of this study was to report a rare case of aspergillus fumigatus infection complicated with cavernous hemangioma in the central nervous system of a patient with normal immune function and to investigate its causes. a 60-year-old male patient was admitted three years ago due to meningioma-induced convulsions. in addition to meningioma, magnetic resonance imaging (mri) results also suggested the presence of cystic and solid lesions in the left temporal lobe, which was considered to be a br ... | 2015 | 26884969 |
| fever and multilobular mass of the right lung in a young adult with asthma. | we report a case of a 37-year-old mild asthmatic male presenting with fever, productive cough and chest pain. the chest x-ray showed a multilobular perihilar shadow of the right lung with a mass-like appearance, confirmed by the ct-scan. he was diagnosed with allergic bronchopulmonary aspergillosis (abpa). abpa usually manifests as chronic asthma, recurrent pulmonary infiltrates and bronchiectasis. however, it can rarely be seen in patients with mild asthma and with an unusual radiological prese ... | 2015 | 26858931 |
| [allergic fungal rhinosinusitis]. | definition, diagnosis and treatment of allergic fungal sinusitis remain difficult and controversial despite the increasing number of publications. we report a case of an allergic fungal sinusitis with a review of the literature. | 2015 | 26603100 |
| improved production of melanin from aspergillus fumigatus afgrd105 by optimization of media factors. | melanins are indolic polymers produced by many genera included among plants, animals and microorganisms and targeted mainly for their wide range of applications in cosmetics, agriculture and medicine. an approach to analyse the cumulative effect of parameters for enhanced melanin production was carried out using response surface methodology. in this present study, optimization of media and process parameters for melanin production from aspergillus fumigatus afgrd105 (genbank: jx041523; nfcci acc ... | 2015 | 26597959 |
| examination of cyp51a and cyp51b expression level of the first polish azole resistant clinical aspergillus fumigatus isolate. | aspergillus fumigatus is one of the most prevalent airborne fungal pathogens causing infections worldwide. most a. fumigatus strains are susceptible to azoles, which are administered as the first line therapeutics. however, during last decade the acquired resistance to triazoles by these species has been described. there is a number of publications concerning the examination of clinical a. fumigatus strains from different countries, however there has been no report from poland. here, we describe ... | 2015 | 26636140 |
| [azole resistance with environmental origin: what alternatives for the future?]. | azole resistant aspergillus fumigatus strains are increasingly reported in many countries. one resistance mechanism is attributed to the use of azole fungicides in environment. two mutations, tr34/l98h and tr46/y121f/t289a, on the cyp51a gene, have been described. results of 40 publications about azole resistant strain detections, with tr34/l98h and tr46/y121f/t289a mutations, in clinical and/or environmental samples, are presented in this review. these cases, observed in many countries, suggest ... | 2015 | 26631951 |
| polyketides from an endophytic aspergillus fumigatus isolate inhibit the growth of mycobacterium tuberculosis and mrsa. | the crude extract of aspergillusfumigatus isolate af3-093a, an endophyte of the brown alga fucus vesiculosus, showed significant antimicrobial activity in initial bioactivity screens. bioassay-guided fractionation of the extract led to the isolation of flavipin, chaetoglobosin a and chaetoglobosin b, all of which inhibited the growth of staphylococcus aureus, methicillin-resistant s. aureus and mycobacterium tuberculosis h37ra. the antimycobacterial activity of these compounds has not been previ ... | 2015 | 26669098 |
| murine models of aspergillosis: role of collectins in host defense. | aspergillus fumigatus, a ubiquitous fungus, causes a wide spectrum of clinical conditions ranging from allergic to invasive aspergillosis depending upon the hosts' immune status. several animal models have been generated to mimic the human clinical conditions in allergic and invasive aspergillosis. the onset, duration and severity of the disease developed in models varied depending on the animal strain/fungal isolate, quantity and mode of administration of fungal antigens/spores, duration of the ... | 2015 | 26669011 |
| the toxicity of a novel antifungal compound is modulated by endoplasmic reticulum-associated protein degradation components. | in a search for new antifungal compounds, we screened a library of 4,454 chemicals for toxicity against the human fungal pathogen aspergillus fumigatus. we identified sr7575, a molecule that inhibits growth of the evolutionary distant fungi a. fumigatus, cryptococcus neoformans, candida albicans, and saccharomyces cerevisiae but lacks acute toxicity for mammalian cells. to gain insight into the mode of inhibition, sr7575 was screened against 4,885 s. cerevisiae mutants from the systematic collec ... | 2015 | 26666917 |
| autoimmune pulmonary alveolar proteinosis following pulmonary aspergillosis. | a 59-year-old man, ex-smoker, was diagnosed with pulmonary aspergillosis according to chest radiography findings of a fungus ball and aspergillus fumigatus detection in the bronchial lavage fluid. two years after anti-fungal therapy, he was diagnosed with autoimmune pulmonary alveolar proteinosis (apap) according to a crazy paving pattern in computed tomography scans of the chest, milky bronchoalveolar lavage effluent, and positive anti-granulocyte-macrophage colony-stimulating factor (gm-csf) a ... | 2015 | 26666607 |
| erratum: secondary metabolites from aspergillus fumigatus, an endophytic fungus from the liverwort heteroscyphus tener (steph.) schiffn. | 2015 | 26663845 | |
| characterization of the paracoccidioides hypoxia response reveals new insights into pathogenesis mechanisms of this important human pathogenic fungus. | hypoxic microenvironments are generated during fungal infection. it has been described that to survive in the human host, fungi must also tolerate and overcome in vivo microenvironmental stress conditions including low oxygen tension; however nothing is known how paracoccidioides species respond to hypoxia. the genus paracoccidioides comprises human thermal dimorphic fungi and are causative agents of paracoccidioidomycosis (pcm), an important mycosis in latin america. | 2015 | 26659387 |
| challenges and strategies for proteome analysis of the interaction of human pathogenic fungi with host immune cells. | opportunistic human pathogenic fungi including the saprotrophic mold aspergillus fumigatus and the human commensal candida albicans can cause severe fungal infections in immunocompromised or critically ill patients. the first line of defense against opportunistic fungal pathogens is the innate immune system. phagocytes such as macrophages, neutrophils and dendritic cells are an important pillar of the innate immune response and have evolved versatile defense strategies against microbial pathogen ... | 2015 | 28248281 |
| calcineurin orchestrates hyphal growth, septation, drug resistance and pathogenesis of aspergillus fumigatus: where do we go from here? | studies on fungal pathogens belonging to the ascomycota phylum are critical given the ubiquity and frequency with which these fungi cause infections in humans. among these species, aspergillus fumigatus causes invasive aspergillosis, a leading cause of death in immunocompromised patients. fundamental to a. fumigatus pathogenesis is hyphal growth. however, the precise mechanisms underlying hyphal growth and virulence are poorly understood. over the past 10 years, our research towards the identifi ... | 2015 | 26694470 |
| late-onset aspergillus fumigatus infection of an aortic stent graft in an immunocompetent patient. | aspergillus fumigatus as a clinical entity is difficult to diagnose. we present a case, which could facilitate diagnosis and management of the aforementioned disease. a 60-year-old man with stent graft implantation in the descending aorta (6 years ago) presented with fever, night sweats, and weight loss over 5 months. leukocytosis and elevated c-reactive protein were constantly spiking. blood cultures were negative. notably, the serum immunoglobulin e (ige) level was strongly elevated (> 1,000 u ... | 2015 | 26693131 |
| up-regulation of dectin-1 in airway epithelial cells promotes mice defense against invasive pulmonary aspergillosis. | with the growing number of immunocompromised patients, the incidence of invasive pulmonary aspergillosis increases. innate immunity plays a significant role in defensing against fungal infection. airway epithelial cells induce immune responses like the production of cytokine and chemokine via dectin-1 signaling pathway in response to aspergillus fumigatus. thus, we hypothesized that up-regulation of dectin-1 on airway epithelium cells would promote the defense against a. fumigatus. | 2015 | 26770339 |
| a probable drug-to-drug interaction between voriconazole and haloperidol in a cyp2c19 poor metabolizing patient. [corrected]. | we present a case of aspergillus fumigatus renal abscess treated with voriconazole. following haloperidol treatment we observed an unexpected increase in voriconazole--trough concentrations and liver function tests. cyp2c19*2 loss of function allele was stated and the introduction of haloperidol, a weak cyp3a4 inhibitor, probably explains this interaction. [corrected]. therapeutic drug monitoring and cyp2c19 genotyping may be suggested when administering voriconazole to complex patients. | 2015 | 26700090 |
| new commercially available igg kits and time-resolved fluorometric ige assay for diagnosis of allergic bronchopulmonary aspergillosis in patients with cystic fibrosis. | allergic bronchopulmonary aspergillosis (abpa) is difficult to diagnose; diagnosis relies on clinical, radiological, pathological, and serological criteria. our aim was to assess the performance of two new commercially available kits and a new in-house assay: an aspergillus fumigatus enzyme-linked immunosorbent assay (elisa) igg kit (bordier affinity products), an aspergillus western blotting igg kit (ldbio diagnostics), and a new in-house time-resolved fluorometric ige assay (dissociation-enhan ... | 2015 | 26698651 |
| cytotoxic and antifungal constituents isolated from the metabolites of endophytic fungus do14 from dendrobium officinale. | two novel cytotoxic and antifungal constituents, (4s,6s)-6-[(1s,2r)-1, 2-dihydroxybutyl]-4-hydroxy-4-methoxytetrahydro-2h-pyran-2-one (1), (6s,2e)-6-hydroxy-3-methoxy-5-oxodec-2-enoic acid (2), together with three known compounds, ll-p880γ (3), ll-p880α (4), and ergosta-5,7,22-trien-3b-ol (5) were isolated from the metabolites of endophytic fungi from dendrobium officinale. the chemical structures were determined based on spectroscopic methods. all the isolated compounds 1-5 were evaluated by cy ... | 2015 | 26703552 |
| [nasal, pulmonary, and abomasal aspergillosis (aspergillus fumigatus) in a calf]. | this study presents a case of nasal aspergillosis in a 17-days old calf (german fleckvieh): it had been admitted moribund to the clinic for ruminants of the university of munich, and died after a short time. pathologically, the calf was diagnosed with purulent-necrotizing rhinitis, necrotizing pneumonia, and diphtheroid-necrotizing abomasitis. histologically, fungal elements were found in all the localizations mentioned before, and mycologically, aspergillus fumigatus was cultured from nasal cav ... | 2015 | 26753360 |
| [coinfection of rhinocerebral mucormycosis and sinus aspergillosis]. | mucormycosis and aspergillosis are the most frequent fungal infections caused by filamentous fungi; coinfection in the same host is rare. we present a case of a 78-year-old male patient with the debut of type 2 diabetes mellitus and ketoacidosis, with swelling of the right side of the face, right facial paralysis, ptosis and a necrotic ulcer in the right palate. facial computed tomography showed an abscess of the right maxillary sinus. cultured secretions revealed aspergillus fumigatus. the path ... | 2015 | 26732934 |
| terpenoids from the marine-derived fungus aspergillus fumigatus yk-7. | two new β-bergamotane sesquiterpenoids, e-β-trans-5,8,11-trihydroxybergamot-9-ene (1) and β-trans-2β,5,15-trihydroxybergamot-10-ene (2), were isolated from the marine-derived fungus aspergillus fumigatus yk-7, along with three known terpenoids 3-5. their structures were determined by spectroscopic methods (1d and 2d nmr, hr-esi-ms). antiproliferative effects on human leukemic monocyte lymphoma u937 and human prostate cancer pc-3 cell lines were measured in vitro. compound 4 exhibited potent acti ... | 2015 | 26729074 |
| extrolites of aspergillus fumigatus and other pathogenic species in aspergillus section fumigati. | aspergillus fumigatus is an important opportunistic human pathogen known for its production of a large array of extrolites. up to 63 species have been described in aspergillus section fumigati, some of which have also been reliably reported to be pathogenic, including a. felis, a. fischeri, a. fumigatiaffinis, a. fumisynnematus, a. hiratsukae, a. laciniosus, a. lentulus, a. novofumigatus, a. parafelis, a. pseudofelis, a. pseudoviridinutans, a. spinosus, a. thermomutatus, and a. udagawae. these s ... | 2015 | 26779142 |
| interstitial lung disease in systemic scleroderma, complicated with bilateral pulmonary aspergilloma: an unusual association. | aspergilloma or mycetoma is a saprophytic fungal infection that colonizes pre-existing excavated lung lesion. however, its association with systemic sclerosis related interstitial lung disease is unusual and scarcely found in literature. we report a middle aged female with long standing systemic sclerosis, who was on immunosuppressive therapy for many years, presented with repeated haemoptysis. although provisionally pulmonary tuberculosis was suspected, imaging investigations showed presence of ... | 2015 | 26816937 |
| interferon-γ promotes phagocytosis of cryptococcus neoformans but not cryptococcus gattii by murine macrophages. | among invasive fungal infections, cryptococcosis caused by inhalation of cryptococcus neoformans or cryptococcus gattii is particularly dangerous because it can disseminate to the central nervous system and cause life-threatening meningitis or meningoencephalitis. previous reports described significant differences in the histopathological features of c. neoformans and c. gattii infection, such as greater pathogen proliferation and a limited macrophage response in mouse lung infected by c. gattii ... | 2015 | 26477011 |
| e-cadherin mediates adhesion of aspergillus fumigatus to non-small cell lung cancer cells. | a spergillus fumigatus is a widely distributed microorganism, and recently, a. fumigatus culture filtrate has been shown to trigger apoptotic cell death in several human cancer cell lines, including non-small lung cancer (nsclc) cells, a549. nevertheless, the molecular adhesion of a. fumigatus to these cancer cells to trigger cell death remains unknown. here, we knocked down e-cadherin in a549 cells and examined its effects on a. fumigatus. the blastospores of a. fumigatus were incubated with th ... | 2015 | 26472726 |
| erratum for nazik et al., effects of iron chelators on the formation and development of aspergillus fumigatus biofilm. | 2015 | 26464401 | |
| production and characterization of two major aspergillus oryzae secreted prolyl endopeptidases able to efficiently digest proline-rich peptides of gliadin. | prolyl endopeptidases are key enzymes in the digestion of proline-rich proteins. fungal extracts rich in prolyl endopeptidases produced by a species such as aspergillus oryzae used in food fermentation would be of particular interest for the development of an oral enzyme therapy product in patients affected by intolerance to gluten. two major a. oryzae secreted prolyl endopeptidases of the merops s28 peptidase family, aos28a and aos28b, were identified when this fungus was grown at acidic ph in ... | 2015 | 26464108 |
| in vitro biochemical study of cyp51-mediated azole resistance in aspergillus fumigatus. | the incidence of triazole-resistant aspergillus infections is increasing worldwide, often mediated through mutations in the cyp51a amino acid sequence. new classes of azole-based drugs are required to combat the increasing resistance to existing triazole therapeutics. in this study, a cyp51 reconstitution assay is described consisting of eburicol, purified recombinant aspergillus fumigatus cpr1 (afcpr1), and escherichia coli membrane suspensions containing recombinant a. fumigatus cyp51 proteins ... | 2015 | 26459890 |