Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| quantization of ph: evidence for acidic activity of triglyceride lipases. | here we present a study of lipolytic activity of lipases from fusarium solani pisi (cutinase), rhizomucor miehei, pseudomonas cepacia, and humicola lanuginosa. their activities toward triolein provide clear evidence for considerable enzymatic activity under acidic conditions. the activity was followed using fourier transform infrared attenuated total reflection (ftir-atr) and nuclear magnetic resonance (nmr). using these approaches, all the lipases that were studied exhibited lipolytic activity ... | 2005 | 16114894 |
| enzymatic surface modification of poly(ethylene terephthalate). | this study unambiguously confirms hydrolysis using cutinase of the persistent synthetic polymer poly(ethylene terephthalate), the most important synthetic fiber in the textile industry by direct measurement and identification of the different hydrolysis products. in this aqueous heterogeneous system, dissolved cutinase from fusarium solani pisi acts on different solid poly(ethylene terephthalate) substrates. the extent of hydrolysis was detected by measuring the amount of (soluble) degradation p ... | 2005 | 16115695 |
| synthesis and evaluation of demethoxyviridin derivatives as potential antimicrobials. | the in vitro antibacterial and antifungal activities of demethoxyviridin and some synthetic analogues were evaluated by the agar diffusion method. the minimum inhibitory concentrations (mic) of the active compounds were also determined by the agar dilution method. demethoxyviridin (1) showed moderate antibacterial activity against most of the strains tested. 1alpha-hydroxydemethoxyviridin (3) showed antibacterial activity and the most potent in vitro antifungal activity with mic of 20 microg/ml ... | 2005 | 16320609 |
| biological activity of spatoglossum asperum: a brown alga. | chloroform and methanol fractions of an ethanol extract of spatoglossum asperum showed antifungal activity against the highly destructive plant pathogen, macrophomina phaseolina while the n-hexane fraction showed activity against rhizoctonia solani and fusarium solani. n-hexane and methanol fractions also showed nematicidal activity against the plant parasitic nematode, meloidogyne javanica. column chromatography of the ethanol extract of seaweed yielded oily fractions named sa-i to sa-vi. gc-ms ... | 2005 | 16161033 |
| effect of voriconazole combined with micafungin against candida, aspergillus, and scedosporium spp. and fusarium solani. | effects of voriconazole combined with micafungin against 101 isolates of candida spp. and 100 isolates of filamentous fungi have been evaluated by in vitro checkerboard analysis. the combination was indifferent for 97% of the candida isolates and synergistic for 64% of the filamentous fungi (79% for aspergillus fumigatus). | 2005 | 16304192 |
| methyl tert-butyl ether and tert-butyl alcohol degradation by fusarium solani. | fusarium solani degraded methyl tert-butyl ether (mtbe) and other oxygenated compounds from gasoline including tert-butyl alcohol (tba). the maximum degradation rate of mtbe was 16 mg protein h and 46 mg/g protein h for tba. the culture transformed 77% of the total carbon to 14co2. the estimated yield for mtbe was 0.18 g dry wt/g mtbe. | 2005 | 16314973 |
| substrate specificity and kinetic properties of enzymes belonging to the hormone-sensitive lipase family: comparison with non-lipolytic and lipolytic carboxylesterases. | we have studied the kinetics of hydrolysis of triacylglycerols, vinyl esters and p-nitrophenyl butyrate by four carboxylesterases of the hsl family, namely recombinant human hormone-sensitive lipase (hsl), est2 from alicyclobacillus acidocaldarius, afest from archeoglobus fulgidus, and protein rv1399c from mycobacterium tuberculosis. the kinetic properties of enzymes of the hsl family have been compared to those of a series of lipolytic and non-lipolytic carboxylesterases including human pancrea ... | 2005 | 16325466 |
| fusarium solani breast abscess. | an unusual manifestation of breast fusariosis was encountered in a 55-year-old female diabetic patient. two fine needle aspirates (fna) from the abscess were done at three days interval and they showed hyaline, septate, branched, fungal hyphae in 10% potassium hydroxide mount. fungal infection was confirmed by demonstrating the fungal hyphae in the midst of lymphocytes, macrophages and neutrophils in leishman stained smears. culture of both fnas yielded a heavy and pure growth of fusarium solani ... | 2005 | 16100431 |
| roles of adherence and matrix metalloproteinases in growth patterns of fungal pathogens in cornea. | to investigate the roles of adherence and matrix metalloproteinases (mmps) in growth patterns of major fungal pathogens in cornea. | 2005 | 16109640 |
| clinical experience in managing fusarium solani keratitis. | fusarium solani keratitis is a rare ocular infectious disease. the clinical characteristics and treatment methods of 18 patients with culture proven f. solani keratitis between july 1997 and december 2003 and with a follow-up period of more than 4 months were analysed retrospectively. the patients were divided into two groups based on the severity of keratitis. group a (n = 13) displayed non-severe keratitis and were treated with debridement, lamellar keratectomy and antifungal medication. group ... | 2005 | 15857351 |
| pathogen-induced expression of a cecropin a-melittin antimicrobial peptide gene confers antifungal resistance in transgenic tobacco. | expression of defensive genes from a promoter that is specifically activated in response to pathogen invasion is highly desirable for engineering disease-resistant plants. a plant transformation vector was constructed with transcriptional fusion between the pathogen-responsive win3.12t promoter from poplar and the gene encoding the novel cecropin a-melittin hybrid peptide (cema) with strong antimicrobial activity. this promoter-transgene combination was evaluated in transgenic tobacco (nicotiana ... | 2005 | 15863447 |
| real-time detection of common microbial volatile organic compounds from medically important fungi by selected ion flow tube-mass spectrometry (sift-ms). | we describe a new method, selected ion flow tube-mass spectrometry (sift-ms) for the rapid and sensitive real-time detection and quantification of volatile organic compounds from medically important fungi, grown on a range of laboratory media. sift-ms utilises the chemical ionisation reactions of mass-selected ions to characterise volatile organic compounds (vocs) that are produced as metabolites from fungi. this technique has the distinct advantage over others in that it readily detects low mol ... | 2005 | 15893831 |
| organometallic-based antibacterial and antifungal compounds: transition metal complexes of 1,1'-diacetylferrocene-derived thiocarbohydrazone, carbohydrazone, thiosemicarbazone and semicarbazone. | organometallic-based, 1,1'-diacetylferrocene-derived antibacterial and antifungal thiocarbohydrazone, carbohydrazone, thiosemicarbazone and semicarbazone have been prepared by condensing equimolar amount of 1,1'-diacetylferrocene with thiocarbohydrazide, carbohydrazide thiosemicarbazide and semicarbazide, respectively. these were used as ligands for the preparation of their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) metal complexes. all the synthesized ligands and their complexes were c ... | 2005 | 15895689 |
| removal of n-hexane by fusarium solani with a gas-phase biofilter. | a gas-phase biofilter inoculated with the fungus fusarium solani, isolated from a consortium grown on hexane vapors, was used to degrade this compound. the biofilter, packed with perlite and operated with an empty bed residence time of 60 s, was supplied with hexane concentrations between 0.5 gm(-3) and 11 gm(-3). biofilter performance was evaluated over 100 days of operation. several strategies for supplying the nutritive mineral medium were assayed to maintain favorable conditions for the fung ... | 2005 | 15933872 |
| assessing the pathogenic effect of fusarium, geosmithia and ophiostoma fungi from broad-leaved trees. | phytopathogenic effect of geosmithia pallida, g. langdonii, ophiostoma grandicarpum, o. querci, two isolates of o. piceae, and two isolates of fusarium solani was compared using plant growth test (stem and root length of garden cress plants seeded on mycelium-covered potato carrot agar); ophiostoma spp. and f. solani were isolated from oak, geosmithia spp. from galleries of scolytus intricatus on beech. all fungi inhibited more the root elongation than that of stems. f. solani led to plant colla ... | 2005 | 15954534 |
| in-vitro antibacterial, antifungal and cytotoxic properties of sulfonamide--derived schiff's bases and their metal complexes. | a series of new antibacterial and antifungal schiff's bases derived from sulfonamides, as well as their transition metal complexes incorporating cobalt (ii), copper (ii), nickel (ii) and zinc (ii) were synthesized, characterized and screened for their in-vitro antibacterial activity against six gram-negative (escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi and shigella dysentriae) and four gram-positive (bacillus cereus, corynebacterium diphth ... | 2005 | 15968823 |
| fungal keratitis due to fusarium solani. | a young man presented with swelling, congestion and lacrimation of right eye for one week. on examination, he had corneal ulcer. corneal scrapings on lactophenol cotton blue stain revealed fungal hyphae. culture on sabouraud agar yielded fusarium solani after 48 hours. patient was treated with ketoconazole and miconazole and responded well. | 2005 | 16181584 |
| in-vitro antibacterial, antifungal and cytotoxic activities of some coumarins and their metal complexes. | a series of new antibacterial and antifungal coumarin-derived compounds and their transition metal complexes [cobalt (ii), copper (ii), nickel (ii) and zinc (ii)] have been synthesized, characterized and screened for their in vitro antibacterial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebacterium diphtheriae, staphylococcus aureus and streptococcus pyogenes bacterial strains ... | 2005 | 16206827 |
| quantitative assessment of phytopathogenic fungi in various substrates using a dna macroarray. | detection, identification and quantification of plant pathogens are the cornerstones of preventive plant disease management. to detect multiple pathogens in a single assay, dna array technology currently is the most suitable technique. however, for sensitive detection, polymerase chain reaction (pcr) amplification before array hybridization is required. to evaluate whether dna array technology can be used to simultaneously detect and quantify multiple pathogens, a dna macroarray was designed and ... | 2005 | 16232285 |
| evaluation of fusarium solani hyphae and conidia susceptibility to amphotericin b and itraconazole: study of a clinical case. | fusarium species are hyaline moulds belonging to the hyalohyphomycosis group that are usually found in the soil and plants. this organism has emerged as a cause of disseminated invasive disease. the correlation between in vitro value and clinical efficacy is low and many patients remain unresponsive to treatment despite in vitro susceptibility. we determined growth control for fusarium solani using the biocell-tracer system that measures the growth rate of a single fungal hypha, and the effect o ... | 2005 | 16244897 |
| variability of non-mutualistic filamentous fungi associated with atta sexdens rubropilosa nests. | a survey of the filamentous fungi other than the symbiotic one found in association with atta sexdens rubropilosa colonies was carried out. different fungal species (27 taxa) were isolated a few days after treating the workers with toxic baits (sulfluramid; mirex-s), from 40 laboratory and 20 field nests. syncephalastrum racemosum (54%) and escovopsis weberi (21%), trichoderma harzianum (38%) and fusarium oxysporum (23%) were the prevalent species in laboratory and field nests, respectively. acr ... | 2005 | 16475502 |
| the causal agents of damping-off disease of buglosse from iran. | iran is considered a major genetic for medicinal plant in the world. because of this significant diversity and historical background in identification and utilization to remedy human and animal diseases, export of medicinal plant can help to strengthen local as well as natural economy. buglosse (fig. 1) is one of the most important and common medicinal plants in iran and exist as echium amoneum and borago officinalis. this work was conducted in order to identify the causal agent(s) of damping of ... | 2005 | 16637194 |
| reaction of selected soybean cultivars to rhizoctonia root rot and other damping-off disease agents. | eight soybean cultivars; giza 21. giza 22, giza 35, giza 82, giza 83, crawford, holladay and toamo were evaluated to rhizoctonia root rot using agar plate and potted plant techniques. data cleared that, in agar plate assay all soybean cultivars were moderately susceptible (ms), although the differences between them were significant (p=0.05). generally, in potted assay, the reactions were resistant (r) or moderately resistant (mr) to root rots. also, the differences between cultivars were signifi ... | 2005 | 16637203 |
| polycyclic aromatic hydrocarbons storage by fusarium solani in intracellular lipid vesicles. | accumulation and elimination of polycyclic aromatic hydrocarbons (pahs) were studied in the fungus fusarium solani. when the fungus was grown on a synthetic medium containing benzo[a]pyrene, hyphae of f. solani contained numerous lipid vesicles which could be stained by the lipid-specific dyes: sudan iii and rhodamine b. the fluorescence produced by rhodamine b and pah benzo[a]pyrene were at the same locations in the fungal hyphae, indicating that f. solani stored pah in pre-existing lipid vesic ... | 2005 | 15519459 |
| unfolding and inactivation of cutinases by aot and guanidine hydrochloride. | we present a comparative analysis of the unfolding and inactivation of three cutinases in the presence of guanidine hydrochloride (gdnhcl) and bis(2-ethylhexyl) sodium sulfosuccinate (aot). previous investigations have focused on the cutinase from fusarium solani pisi (fsc). in addition to fsc, the present study includes the cutinase from humicola insolens (hic) and a mutant variant of hic (muhic) with increased activity and decreased surfactant sensitivity. equilibrium and time-resolved denatur ... | 2005 | 15752695 |
| chemical composition and antifungal activity of essential oils from different tissues of japanese cedar (cryptomeria japonica). | in this study antifungal activities of essential oils from different tissues of japanese cedar (cryptomeria japonica d. don) against four wood decay fungi and six tree pathogenic fungi were investigated. in addition, the yields of essential oils obtained by water distillation were compared and their constituents determined by gc-ms analyses. the yield of essential oils from four tissues of japanese cedar is in the decreasing order of leaf (27.38 ml/kg) > bark (6.31 ml/kg) > heartwood (3.80 ml/kg ... | 2005 | 15686410 |
| concordant evolution of trichothecene 3-o-acetyltransferase and an rdna species phylogeny of trichothecene-producing and non-producing fusaria and other ascomycetous fungi. | the cereal pathogen fusarium graminearum species complex (e.g. fusarium asiaticum, previously referred to as f. graminearum lineage 6) produces the mycotoxin trichothecene in infected grains. the fungus has a gene for self-defence, tri101, which is responsible for 3-o-acetylation of the trichothecene skeleton in the biosynthetic pathway. recently, trichothecene non-producers fusarium oxysporum and fusarium fujikuroi (teleomorph gibberella fujikuroi) were shown to have both functional (tri201) an ... | 2005 | 15699200 |
| solar and photocatalytic disinfection of protozoan, fungal and bacterial microbes in drinking water. | the ability of solar disinfection (sodis) and solar photocatalytic (tio(2)) disinfection (spc-dis) batch-process reactors to inactivate waterborne protozoan, fungal and bacterial microbes was evaluated. after 8 h simulated solar exposure (870 w/m(2) in the 300 nm-10 microm range, 200 w/m(2) in the 300-400 nm uv range), both spc-dis and sodis achieved at least a 4 log unit reduction in viability against protozoa (the trophozoite stage of acanthamoeba polyphaga), fungi (candida albicans, fusarium ... | 2005 | 15743634 |
| first report of a novel plant lysozyme with both antifungal and antibacterial activities. | a novel lysozyme exhibiting antifungal activity and with a molecular mass of 14.4kda in sds-polyacrylamide gel electrophoresis was isolated from mung bean (phaseolus mungo) seeds using a procedure that involved aqueous extraction, ammonium sulfate precipitation, ion exchange chromatography on cm-sephadex, and high-performance liquid chromatography on poros hs-20. its n-terminal sequence was very different from that of hen egg white lysozyme. its pi was estimated to be above 9.7. the specific act ... | 2005 | 15649419 |
| recurrent colonization of successively implanted tracheoesophageal vocal prostheses by a member of the fusarium solani species complex. | tracheoesophageal vocal prostheses (tvp) in laryngectomized patients commonly deteriorate due to overgrowth by yeasts, particularly candida species. we describe the first case of colonization of such devices by a member of the fusarium solani species complex in a patient with a history of glottal carcinoma. three isolates, from three prostheses, were found morphologically consistent with the traditional picture of f. solani. ribosomal sequence analysis showed that the isolates belonged to a dist ... | 2005 | 15695678 |
| a generic system for the escherichia coli cell-surface display of lipolytic enzymes. | esta is an outer membrane-anchored esterase from pseudomonas aeruginosa. an inactive esta variant was used as an anchoring motif for the escherichia coli cell-surface display of lipolytic enzymes. flow cytometry analysis and measurement of lipase activity revealed that bacillus subtilis lipase lipa, fusarium solani pisi cutinase and one of the largest lipases presently known, namely serratia marcescens lipase were all efficiently exported by the esta autotransporter and also retained their lipol ... | 2005 | 15710409 |
| genomic analysis of a region encompassing qrfs1 and qrfs2: genes that underlie soybean resistance to sudden death syndrome. | candidate genes were identified for two loci, qrfs2 providing resistance to the leaf scorch called soybean (glycine max (l.) merr.) sudden death syndrome (sds) and qrfs1 providing resistance to root infection by the causal pathogen fusarium solani f.sp. glycines. the 7.5 +/- 0.5 cm region of chromosome 18 (linkage group g) was shown to encompass a cluster of resistance loci using recombination events from 4 near-isogenic line populations and 9 dna markers. the dna markers anchored 9 physical map ... | 2005 | 15729404 |
| a chitinase with antifungal activity from the mung bean. | a chitinase with antifungal activity was isolated from mung bean (phaseolus mungo) seeds. the procedure entailed aqueous extraction, (nh4)2so4 precipitation, ion-exchange chromatography on cm-sepharose, high-performance liquid chromatography (hplc) on poros hs-20, and gel filtration on sephadex g-75. the protein exhibited a molecular mass of 30.8 kda in sds-polyacrylamide gel electrophoresis. its pi was 6.3 as determined by isoelectric focusing. the specific activity of the chitinase was estimat ... | 2005 | 15766863 |
| root response to fusarium solani f. sp . glycines: temporal accumulation of transcripts in partially resistant and susceptible soybean. | sudden death syndrome (sds) of soybean is a complex of root rot disease caused by the semi-biotrophic fungus fusarium solani f. sp. glycines (fsg) and a leaf scorch disease caused by toxins produced by the pathogen in the roots. development of partial rate-reducing resistance in roots to sds was studied. the recombinant inbred line 23 (ril23) that carried resistance conferred by six quantitative trait loci (qtl) derived from cultivars 'essex' x 'forrest' was compared to the susceptible cultivar ... | 2005 | 15815926 |
| fragrance release from the surface of branched poly (amide)s. | enzymes are powerful tools in organic synthesis that are able to catalyse a wide variety of selective chemical transformations under mild and environmentally friendly conditions. enzymes such as the lipases have also found applications in the synthesis and degradation of polymeric materials. however, the use of these natural catalysts in the synthesis and the post-synthetic modification of dendrimers and hyperbranched molecules is an application of chemistry yet to be explored extensively. in th ... | 2005 | 18007278 |
| a promoter from pea gene drr206 is suitable to regulate an elicitor-coding gene and develop disease resistance. | abstract plant nonhost disease resistance is characterized by the induction of multiple defense genes. the pea drr206 gene is induced following inoculation with pathogens and treatment with abiotic agents, and moderately induced by wounding. a deletion series of drr206 promoter segments was fused with the beta-glucuronidase (gus) reporter gene and transiently transferred to tobacco, potato, and pea. gus activity revealed that two upstream regions of the drr206 promoter were particularly importan ... | 2004 | 18943490 |
| protein topology affects the appearance of intermediates during the folding of proteins with a flavodoxin-like fold. | the topology of a native protein influences the rate with which it is formed, but does topology affect the appearance of folding intermediates and their specific role in kinetic folding as well? this question is addressed by comparing the folding data recently obtained on apoflavodoxin from azotobacter vinelandii with those available on all three other alpha-beta parallel proteins the kinetic folding mechanism of which has been studied, i.e. anabaena apoflavodoxin, fusarium solani pisi cutinase ... | 2004 | 15829351 |
| pulmonary hyalohyphomycosis caused by fusarium spp in a kemp's ridley sea turtle (lepidochelys kempi): an immunohistochemical study. | to describe the microscopic and immunohistochemical findings in a case of pulmonary hyalohyphomycosis in a kemp's ridley sea turtle (lepidochelys kempi). | 2004 | 15768112 |
| potential ecotoxicological implication of methyl tert-butyl ether (mtbe) spills in the environment. | streptomyceticidal activity of methyl tert-butyl ether (mtbe) elucidated for the first time. adverse effect of mtbe, the gasoline additive, against 11 soil inhabitant streptomyces spp. isolates was investigated. mtbe, an octane enhancer is added to gasoline to reduce atmospheric concentrations of carbon monoxide and ozone. it contaminates soil and groundwater by fuel leaks and spills. streptomyces spp. are of the major contributors to the biological buffering of soils by exerting beneficial and ... | 2004 | 15673212 |
| in vitro susceptibility testing of amorolfine in pathogenic fungi isolated from dermatomycosis patients in china. | the antifungal susceptibility of isolates from chinese dermatomycosis patients to amorolfine was investigated following national committee for clinical laboratory standards (nccls) protocols. in total, 383 isolates were tested, including 132 strains from tinea pedis, 148 strains from tinea corporis/cruris, and 103 strains from onychomycosis. the minimum inhibitory concentration (mic) of amorolfine against dermatophytes ranged from 0.01 to 0.08 microg ml(-1). the mic(50) and mic(90) of amorolfine ... | 2004 | 15504124 |
| fusarium solani endocarditis successfully treated with liposomal amphotericin b and voriconazole. | fungal infections caused by fusarium in the immunocompromised host are highly resistant to all antifungal agents. fusarium endocarditis is a rare and usually fatal disease. we report an immunocompromised child who survived fusarium solani endocarditis despite the in vitro resistance of the organism to all available antifungal agents. | 2004 | 15545866 |
| phenylpropanoids, phenylalanine ammonia lyase and peroxidases in elicitor-challenged cassava (manihot esculenta) suspension cells and leaves. | control of diseases in the key tropical staple, cassava, is dependent on resistant genotypes, but the innate mechanisms are unknown. the aim was to study phenylpropanoids and associated enzymes as possible defence components. | 2004 | 15145789 |
| vesicles as initial skin manifestation of disseminated fusariosis after non-myeloablative stem cell transplantation. | a 52-year-old man underwent non-myeloablative stem cell transplantation from his hla identical sister for the treatment of mantle cell lymphoma. on day 0, he developed a high-grade fever, watery diarrhea and vesicles scattered on the skin. well experienced dermatologists diagnosed these lesions as vzv reactivation. high dose antiviral agents were ineffective, and fusarium solani was cultured from his stool and sputum. systemic fusariosis progressed rapidly and he died of multiorgan failure on da ... | 2004 | 15160931 |
| immunosuppression affects the severity of experimental fusarium solani keratitis. | we have established a mouse model of corneal fusariosis that permits the evaluation of fungal infection and pathogenesis. corneas of immunocompetent and cyclophosphamide-treated adult balb/c mice were topically inoculated with fusarium solani after corneal scarification. eyes were scored for corneal involvement daily for 8 days and at 2 weeks after infection. eyes were enucleated at various time points for quantitative fungal recovery and histopathological examination. an inoculum-dose response ... | 2004 | 15195260 |
| antifungal cobalt(ii), copper(ii), nickel(ii) and zinc(ii) complexes of furanyl-,thiophenyl-, pyrrolyl-, salicylyl- and pyridyl-derived cephalexins. | some novel cephalexin-derived furanyl, thiophenyl, pyrrolyl, salicylyl and pyridyl schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes have been synthesized and studied for their antifungal properties against trichophyton longifusus, candida albicans, aspergillus flavus, microsporum canis, fusarium solani and candida glaberata. the presence of metal ions in the investigated schiff's base complexes reported here lead to significant antifungal activity, whereas t ... | 2004 | 15202498 |
| a non-specific lipid transfer protein with antifungal and antibacterial activities from the mung bean. | a non-specific lipid transfer peptide (nsltp) with antimicrobial activity was isolated from the mung bean (phaseolus mungo) seeds. the procedure entailed aqueous extraction, ion exchange chromatography on cm-sephadex and high performance liquid chromatography (hplc) on poros-hs-20. the peptide exhibited a molecular mass of 9.03 kda in mass spectrometry. it exerted antifungal action toward fusarium solani, fusarium oxysporum, pythium aphanidermatum and sclerotium rolfsii, and antibacterial action ... | 2004 | 15350690 |
| antibacterial and antifugal mono- and di-substituted symmetrical and unsymmetrical triazine-derived schiff-bases and their transition metal complexes. | a new series of antibacterial and antifungal triazine-derived mono- and di-substituted (symmetrical and unsymmetrical) schiff-bases and their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) metal complexes have been synthesized and characterized by their elemental analyses, molar conductances, magnetic moments and ir and electronic spectral measurements. ir spectra indicated the ligands to act as tridentate towards divalent metal ions via a trazine-n, the azomethine-n and, indole-nh and deproton ... | 2004 | 15449731 |
| production in vitro of toxic macromolecules by strains of beauveria bassiana, and purification of a chitosanase-like protein secreted by a melanizing isolate. | the production of macromolecular insecticidal toxins in adamek's medium by two selected strains of beauveria bassiana was investigated. filtrates and dialysates of the melanizing strain 618 were toxic when injected into the lepidopteran insect galleria mellonella. separation by deae chromatography revealed that peaks eluted respectively with 100 and 200 mm nacl (p 100 and p 200) had an insecticidal activity and induced cuticular blackening. a hydrophilic protein, bclp, which causes the formation ... | 2004 | 15450862 |
| isoflavonoid accumulation in soybean hairy roots upon treatment with fusarium solani. | hairy roots were initiated from two soybean [glycine max (l.) merr.] genotypes with different susceptibility (susceptible 'spencer' and partially resistant 'pi567.374') to the disease sudden death syndrome (sds) caused by the soil-borne fungal pathogen fusarium solani f. sp. glycines (fsg) to study the role of isoflavonoids in the plant response to fsg infection. hairy root cultures obtained by transformation with agrobacterium rhizogenes allows normal root growth that can be visually monitored. ... | 2004 | 15331097 |
| effectiveness of contact lens disinfectants after lens storage. | to determine the effectiveness of multipurpose contact lens solutions after incubation with lenses for one week. | 2004 | 15499238 |
| preliminary evidence of the role of hydrogen peroxide in the degradation of benzo[a]pyrene by a non-white rot fungus fusarium solani. | in order to study the enzymatic mechanisms involved in the successive steps of bap degradation by a deuteromycete fungus fusarium solani, we developed an indirect approach by using inhibitors of enzymes. we used either specific inhibitors of peroxidases (i.e. salicylhydroxamic acid) and of cytochrome p-450 (i.e. piperonyl butoxyde) or inhibitors of both enzymes (i.e. potassium cyanide). surprisingly, no expected decrease of bap degradation was observed with most inhibitors tested. on the contrar ... | 2004 | 14749063 |
| purification and characterization of a major cross-reactive allergen from epicoccum purpurascens. | epicoccum purpurascens (formerly nigrum) (ep), is a ubiquitous saprophytic mould found both indoors and outdoors. several studies have reported sensitization to ep in 5-7% of different populations worldwide. the diagnosis of mould allergy requires a standardized fungal extract that contains all its important allergenic proteins. the crude allergen extract from ep was standardized earlier, however none of its allergens have been purified. | 2004 | 14976389 |
| recombinant saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways. | although the metabolism and physiology of the growth of yeast strains has been extensively studied, many questions remain unanswered when the induced production of a recombinant protein is concerned. this work addresses the production of a fusarium solani pisi cutinase by a recombinant saccharomyces cerevisiae strain induced through the use of a galactose promoter. it was observed that whenever the strain needed to activate biosynthetic pathways, either for cutinase synthesis, or for the synthes ... | 2004 | 15063624 |
| antimicrobial and insecticidal protein isolated from seeds of clitoria ternatea, a tropical forage legume. | the tropical forage legume clitoria ternatea (l.) has important agronomic traits such as adaptation to a wide range of soil conditions and resistance to drought. it is resistant to a number of pathogens and pests. these important traits gave us reasons to look more closely at the plant. a highly basic small protein was purified from seeds of c. ternatea to homogeneity by using ultrafiltration with centricon-3 membrane tubes and preparative granulated-bed isoelectric focusing (ief). a single prot ... | 2004 | 15694280 |
| isatin-derived antibacterial and antifungal compounds and their transition metal complexes. | a series of isatins incorporating thiazole, thiadiazole, benzothiazole and p-toluene sulfonyl hydrazide moieties, along with their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) metal complexes have been synthesized and characterized by elemental analyses, molar conductances, magnetic moments, ir, nmr and electronic spectral data. these compounds have been screened for antibacterial activity against escherichia coli, bacillus subtillis, shigella flexneri, staphylococcus aureus, pseudomonas aeru ... | 2004 | 15648656 |
| effect of voriconazole on a corneal abscess caused by fusarium. | to describe a case of corneal abscess caused by fusarium solani that did not respond to common antifungal agents. | 2004 | 15606478 |
| intracranial fusariosis: a novel cause of fungal meningoencephalitis in a dog. | the fusarium species are a group of saprophytic fungal organisms that are occasionally the cause of opportunistic infections in humans and animals. central nervous system disease associated with a fusarium species is most commonly described in horse, resulting in equine leukoencephalomalacia. this report describes a 2-year-old, spayed, female german shepherd dog with meningoencephalitis secondary to infection with fusarium spp. meningoencephalitis in dogs secondary to a species of fusarium has n ... | 2004 | 15347824 |
| genotyping of 44 isolates of fusarium solani, the main agent of fungal keratitis in brazil. | in a retrospective study performed over 6 years in brazil, fusarium solani was found to be the most common species causing mycotic keratitis. the genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus pcr (eric-pcr) and pcr restriction fragment length polymorphism (pcr-rflp) fingerprinting. eric-pcr was more discriminatory than pcr-rflp for differentiating the strains. by combining of the results of both techniques, we identified 40 geno ... | 2004 | 15472299 |
| chemical interactions of brachiaria plantaginea with commelina bengalensis and acanthospermum hispidum in soybean cropping systems. | previous results obtained in soybean-wheat rotations under no-tillage conditions showed reductions in the seedbank of the weed species commelina benghalensis, but no alteration in the seedbank of acanthospermum hispidum in areas infested with brachiaria plantaginea. analyses of the soluble fraction of b. plantaginea indicated the predominance of aconitic acid (aa) among the aliphatic acids and ferulic acid (fa) among the phenolic acids. laboratory bioassays using c. benghalensis and a. hispidum ... | 2004 | 15503532 |
| onychomycosis caused by fusarium solani and fusarium oxysporum in são paulo, brazil. | fusarium species are common soil saprophytes and plant pathogens that have been frequently reported as etiologic agents of opportunistic infections in humans. we report eight cases of onychomycosis caused by fusarium solani (4) and fusarium oxysporum (4) in são paulo, brazil. these species were isolated from toenails in all cases. the infections were initially considered to be caused by dermatophytes. the clinical appearance of the affected toenails was leukonychia or distal subungual hyperkerat ... | 2004 | 15180157 |
| [assessment of airborne mycoflora in critical areas of the principal hospital of cumaná, state of sucre, venezuela]. | the study of the nosocomial infections of fungic origin has attained importance in the last years, due to the rise in the number of patients that are inmunocompromised and susceptible to suffer this kind of infection. the objective of the present study was to evaluate the frequency of filamentous fungi and yeast, present in the environment of the intensive care unit, operating and newborn children rooms of the hospital universitario "antonio patricio de alcalá" (huapa) from the city of cumaná. s ... | 2004 | 15211981 |
| contribution of regimen steps to disinfection of hydrophilic contact lenses. | the effect of regimen steps on overall performance of contact lens disinfection processes was evaluated. hydrophilic lenses were inoculated with staphylococcus aureus, pseudomonas aeruginosa, serratia marcescens, candida albicans, or fusarium solani. contributions of regimen steps and rinse volumes to disinfection abilities of opti-free express, solo-care plus (ciba vision), complete (allergan), and renu multiplus (bausch & lomb) multi-purpose solutions were examined. numbers of surviving microo ... | 2004 | 16303537 |
| mistletoe viscotoxins induce membrane permeabilization and spore death in phytopathogenic fungi. | viscotoxins (vts) are basic peptides expressed in mistletoe leaves, seeds and stems which have been shown to be cytotoxic to mammalian cells. the aim of this study was to analyse whether vts were able to control and/or inhibit the growth of phytopathogenic fungi to obtain a clue to their biological function. incubation of two vt isoforms, vta(3) and vtb, at a final concentration of 10 micro m resulted in a complete blockage of the germination of spores from three different pathogenic fungi. it w ... | 2004 | 15086811 |
| site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex. | protein engineering strategies facilitating controlled and spontaneous assembly of macromolecular complexes are of great interest for the design of artificial multi-enzyme systems of pre-defined composition. here we have combined affinity proteins from different sources to achieve specific and reversible anchoring of affinity domain-tagged reporter proteins to a cellulose-anchored fusion protein. the design principle mimics the architecture of macromolecular cellulosome complexes produced by som ... | 2004 | 15066765 |
| effect of immobilization support, water activity, and enzyme ionization state on cutinase activity and enantioselectivity in organic media. | we studied the reaction between vinyl butyrate and 2-phenyl-1-propanol in acetonitrile catalyzed by fusarium solani pisi cutinase immobilized on zeolites naa and nay and on accurel pa-6. the choice of 2-phenyl-1-propanol was based on modeling studies that suggested moderate cutinase enantioselectivity towards this substrate. with all the supports, initial rates of transesterification were higher at a water activity (a(w)) of 0.2 than at a(w) = 0.7, and the reverse was true for initial rates of h ... | 2004 | 14755562 |
| effect of soil moisture on seed germination, plant growth and root rot severity of navy bean in fusarium solani infested soil. | the development of root rot and its severity were affected by numerous factors which through their interaction could distort results. in this study, a single factor, soil moisture was considered. using fusarium solani infested root rot soil we investigated the effect of three available soil moisture (asm) levels (i.e. 100%, 70% and 25%) on seed rot, germination, plant growth and root rot severity of navy bean in the greenhouse. percent germination differed strikingly averaging of 58.5, 89.5 and ... | 2003 | 15151296 |
| synthesis and antimicrobial activity of n-(substituted)-n'-(2,3-dihydro-2-oxido-5-benzoyl-1h-1,3,2-benzodiazaphosphol-2-yl) ureas. | n-(substituted)-n'-(2,3-dihydro-5-benzoyl-2-oxido-1h-1,3,2-benzodiazaphosphol-2-yl) ureas were synthesized by reacting 3,4-diaminobenzophenone (4) with different chlorides of carbamidophosphoric acids (3) in the presence of triethylamine at 40-45 degrees c. their 1h-, 13c- and 31p-nmr spectral data are discussed. the title compounds were screened for antifungal and antibacterial activity against the fungi aspergillus niger and fusarium solani and bacteria escherichia coli and staphylococcus aure ... | 2003 | 12843597 |
| [disseminated fusarium solani infection in patients undergoing hematopoietic stem cell transplantation]. | a patient with a hematological malignancy is one example of a type of immunocompromised host, and critical opportunistic infections such as mycosis are not rare during medical treatment for such malignancy. candidiasis and aspergillosis are typical mycoses and their importance has been recognized widely and great progress attained in their prevention and medical treatment. however, allogenic hematopoietic stem cell transplantation (allo-hsct) to treat hematological malignancy has spread, and the ... | 2003 | 12913807 |
| anatomy of a regimen: consideration of multipurpose solutions during non-compliant use. | consumers are often non-compliant with instructions for contact lens care products. this study explores the antimicrobial capacity of multipurpose solutions using variable use conditions. opti-free express (alcon) multi-purpose disinfecting solution with polyquad (alcon) and aldox (alcon) antimicrobial system and products containing phmb (renu multiplus, solo-care, and complete multipurpose solutions) were evaluated. products were challenged with fusarium solani, candida albicans, serratia marce ... | 2003 | 16303493 |
| effects of codon usage versus putative 5'-mrna structure on the expression of fusarium solani cutinase in the escherichia coli cytoplasm. | matching the codon usage of recombinant genes to that of the expression host is a common strategy for increasing the expression of heterologous proteins in bacteria. however, while developing a cytoplasmic expression system for fusarium solani cutinase in escherichia coli, we found that altering codons to those preferred by e. coli led to significantly lower expression compared to the wild-type fungal gene, despite the presence of several rare e. coli codons in the fungal sequence. on the other ... | 2003 | 12509995 |
| antifungal effects of different organic extracts from melia azedarach l. on phytopathogenic fungi and their isolated active components. | extracts from different parts of melia azedarach l. were studied as potential antifungal agents for selected phytopathogenic fungi. in a serial agar dilution method, hexanic and ethanolic extracts from fruit, seed kernels, and senescent leaves exhibited fungistatic activity against aspergillus flavus,diaporthe phaseolorum var. meridionales, fusarium oxysporum, fusarium solani, fusarium verticillioides, and sclerotinia sclerotiorum. both hexanic extract from senescent leaves and ethanolic extract ... | 2003 | 12696928 |
| advanced fusarium keratitis progressing to endophthalmitis. | to review the clinical course, treatment, and visual outcomes of keratitis with endophthalmitis caused by the filamentary fungus fusarium. | 2003 | 12792470 |
| pentamidine is active in vitro against fusarium species. | fusariosis is an emerging opportunistic mycosis against which currently used antifungals have limited activity. here, we investigated the in vitro activities of pentamidine (pnt) against 10 clinical isolates of fusarium species (five fusarium solani isolates and five non-f. solani isolates) by using the national committee for clinical laboratory standards microdilution method in three different media (rpmi, rpmi-2, and a yeast nitrogen base medium), disk diffusion testing, and viability dye stai ... | 2003 | 14506038 |
| fluorescence of the single tryptophan of cutinase: temperature and ph effect on protein conformation and dynamics. | the cutinase from fusarium solani pisi is an enzyme with a single l-tryptophan (trp) involved in a hydrogen bond with an alanine (ala) residue and located close to a cystine formed by a disulfide bridge between two cysteine (cys) residues. the cys strongly quenches the fluorescence of trp by both static and dynamic quenching mechanisms. the trp fluorescence intensity increases by about fourfold on protein melting because of the disruption of the ala-trp hydrogen bond that releases the trp from t ... | 2003 | 12929743 |
| improvement of phenylethanoid glycosides production by a fungal elicitor in cell suspension culture of cistanche deserticola. | when, on the 15th day of growth, an elicitor from fusarium solani was added at 40 mg l(-1) to cistanche deserticola cell suspension cultures, the contents of echinacoside, acteoside and total phenylethanoid glycosides (pegs) in cultured cells all increased over the next 27 d by over 100% to 15 mg g(-1) dry wt, 9 mg g(-1) dry wt and 57 mg g(-1) dry wt, respectively. the final biomass (1.3 mg dry wt ml(-1)) was not affected. | 2003 | 14514046 |
| [phytotoxic activity of chernozem saprophytic micromycetes: specificity, sorption and stability of phytotoxins in soil]. | micromycetes of the complex of typical chernozem saprotrophic fungi released phytotoxic metabolites into medium. the metabolites displayed their phytotoxic activities directly in soil. evaluation of the toxicities, range of biological effects activities, and stabilities of phytotoxins in soil and the rates of their biodegradation allowed the species that can serve as indicators of chernozem microbial toxicosis to be selected, namely, aspergillus clavatus, fusarium solani, talaromyces flavus, pen ... | 2003 | 14520964 |
| successful voriconazole therapy of disseminated fusarium solani in the brain of a neutropenic cancer patient. | 2003 | 14581897 | |
| two unrelated double-stranded rna molecule patterns in gremmeniella abietina type a code for putative viruses of the families totiviridae and partitiviridae. | two double stranded (ds) rna molecule patterns, probably of viral origin, were sequenced from gremmeniella abietina var. abietina type a. the genome of gremmeniella abietina rna virus l1 (garv-l1) from isolate hr2 was 5133 bp and contained two open reading frames (orfs). the 5'-proximal orf coded for a putative coat protein (cp) and the 3'-proximal orf encoded putative rna-dependent rna polymerase (rdrp). garv-l1 had sequence similarities with a previously described totivirus (helminthosporium v ... | 2003 | 14648287 |
| fusarium solani infection complicated by tuberculous omarthritis. | 2003 | 14673299 | |
| isolation and characterization of a lectin from annona muricata seeds. | a lectin with a high affinity for glucose/mannose was isolated from annona muricata seeds (annonaceae) by gel filtration chromatography on sephacryl s-200, ion exchange chromatography on a deae sp-5 pw column, and molecular exclusion on a protein pak glass 300 sw column. sodium dodecyl sulfate polyacrylamide gel electrophoresis (page) yielded two protein bands of approximately 14 kda and 22 kda. however, only one band was seen in native page. the mr of the lectin estimated by fast-performance li ... | 2003 | 14714732 |
| fusarium osteomyelitis of the foot in a patient with diabetes mellitus. | diabetic foot osteomyelitis is among the most common and serious complications in patients with diabetes mellitus. it is often a polymicrobial infection. we report the first case of foot osteomyelitis in a diabetic patient caused by fusarium solani. | 2003 | 14723375 |
| interactions of human phagocytes with moulds fusarium spp. and verticillium nigrescens possessing different pathogenicity. | fusarium spp. are emerging as important causes of invasive fungal infections. they tend to have decreased susceptibility to antifungal agents, making host defences very important. the ability of human phagocytes to cause damage to hyphae of fusarium solani, f. oxysporum and verticillium nigrescens, a mould with very low pathogenicity, was assessed using the 2,3-bis[2-methoxy-4-nitro-5-sulphophenyl]2h-tetrazolium-5-carboxanilide (xtt) metabolic assay. the oxidative burst, evidenced as superoxide ... | 2003 | 14725324 |
| physicochemical characterization and antimicrobial properties of rhamnolipids produced by pseudomonas aeruginosa 47t2 ncbim 40044. | pseudomonas aeruginosa 47t2, grown in submerged culture with waste frying oil as a carbon source, produced a mixture of rhamnolipids with surface activity. up to 11 rhamnolipid homologs (rha-rha-c(8)-c(10); rha-c(10)-c(8)/rha-c(8)-c(10);rha-rha-c(8)-c(12:1); rha-rha-c(10)-c(10); rha-rha-c(10)-c(12:1); rha-c(10)-c(10); rha-rha-c(10)-c(12)/rha-rha-c(12)-c(10); rha-c(10)-c(12:1)/rha-c(12:1)-c(10); rha-rha-c(12:1)-c(12); rha-rha-c(10)-c(14:1); rha-c(10)-c(12)/rha-c(12)-c(10)) were isolated from cult ... | 2003 | 12474254 |
| localized cutaneous hyalohyphomycosis caused by a fusarium species infection in a renal transplant patient. | fusariosis is a hyalohyphomycosis due to fusarium species that mainly occurs in immunocompromised hosts. the clinical spectrum of fusarium infection comprises localized and disseminated forms. a case of localized cutaneous fusariosis caused by fusarium solani in a renal transplant patient is described, and the skin manifestations of the disease are discussed. | 2003 | 12574313 |
| activity of the amidoamine myristamidopropyl dimethylamine against keratitis pathogens. | microbial keratitis accounts for up to 30% of blindness in some less developed societies. the development of a single broad-spectrum topical antimicrobial effective against bacteria, fungi and acanthamoeba would have a major impact on reducing the morbidity and simplifying the treatment of microbial keratitis. to this end, the activity of the amidoamine myristamidopropyl dimethylamine (mapd) was investigated against common causes of microbial keratitis. | 2003 | 12716783 |
| activity, conformation and dynamics of cutinase adsorbed on poly(methyl methacrylate) latex particles. | the adsorption of a recombinant cutinase from fusarium solani pisi onto the surface of 100 nm diameter poly(methyl methacrylate) (pmma) latex particles was evaluated. adsorption of cutinase is a fast process since more than 70% of protein molecules are adsorbed onto pmma at time zero of experiment, irrespective of the tested conditions. a langmuir-type model fitted both protein and enzyme activity isotherms at 25 degrees c. gamma(max) increased from 1.1 to 1.7 mg m(-2) and u(max) increased from ... | 2003 | 12730007 |
| volatile metabolites from salvia fruticosa as antifungal agents in soilborne pathogens. | the volatile metabolites of salvia fruticosa plants, growing wild in 15 localities scattered across greece, were analyzed by means of gc and gc-ms. the essential oil content ranged from 0.69 to 4.68%, and the results of the analyses showed a noticeable variation in the amounts of the five main components [1,8-cineole, alpha-thujone, beta-thujone, camphor, and (e)-caryophyllene]. the antifungal activities of the essential oils from two localities, belonging in two different groups of cluster and ... | 2003 | 12744657 |
| improving the mouse model for studying the efficacy of voriconazole. | outbred icr mice were rendered neutropenic, infected intravenously with fusarium solani and treated orally with voriconazole. when given alone, voriconazole was not protective up to 40 mg/kg/day. when grapefruit juice was administered before infection, mice were protected by voriconazole. the mechanism may be inhibition of gut mucosal cytochrome enzymes that rapidly degrade voriconazole in the mouse. these murine studies support expansion of voriconazole therapy in other highly resistant systemi ... | 2003 | 12746374 |
| non-pathogenic fusarium solani represses the biosynthesis of nematicidal compounds in vitro and reduces the biocontrol of meloidogyne javanica by pseudomonas fluorescens in tomato. | the aim of the present investigation was to determine the influence of various fusarium solani strains on the production of nematicidal agent(s) in vitro and biocontrol of meloidogyne javanica in tomato by pseudomonas fluorescens strains cha0 and cha0/pme3424. | 2003 | 12859651 |
| a binuclear zinc transcription factor binds the host isoflavonoid-responsive element in a fungal cytochrome p450 gene responsible for detoxification. | the pda1 gene of the filamentous fungus nectria haematococca mpvi (anamorph: fusarium solani) encodes pisatin demethylase, a cytochrome p450. pisatin is a fungistatic isoflavonoid produced by garden pea (pisum sativum), a host for this fungus. pisatin demethylase detoxifies pisatin and functions as a virulence factor for this fungus. pisatin induces pda1 expression both in cultured mycelia as well as during pathogenesis on pea. the regulatory element within pda1 that provides pisatin-responsive ... | 2003 | 12823815 |
| [post traumatic subcutaneous mycosis due to fusarium solani]. | we report the case of a subcutaneous hyalohyphomycosis of a 24-year-old man, a rural worker with an ulcerative lesion in the right leg of approximately one year duration. it was caused by traumatic implantation of a yerba mate branch. the diagnosis was made by direct microscopic examination with 20% potassium hydroxide (koh) and revealed several septate hyaline hyphae. it was confirmed by culture of several samples where fusarium solani was isolated. the patient received local and systemic antif ... | 2003 | 12825979 |
| experimental murine hyalohyphomycosis with soil-derived isolates of fusarium solani. | two strains of soil-borne fusarium solani, both characterized for their ability to produce cyclosporin a and c, were examined for their pathogenicity in severe combined immunodeficiency (scid) and balb/c male mice. intravenous (i.v.) infections with f. solani conidia were performed. no mortality was observed after infection with 0.3-1.6 x 10(7) cfu per mouse in scid and balb/c mice. when mice were infected with 0.8-1.5 x 10(6) cfu per mouse and 2 days later with 1.2-1.9 x 10(6) cfu per mouse, 28 ... | 2003 | 12964716 |
| monacrosporium janus sp. nov., a new nematode-trapping hyphomycete parasitizing sclerotia and hyphae of sclerotinia sclerotiorum. | during an investigation of mycoparasitic fungi on sclerotia of sclerotinia sclerotiorum in china, a new fungal species was consistently encountered and isolated from natural soils taken from soybean fields of shandong and jiangsu provinces. the fungus is featured by its sphaeroid conidia with 1-2 transverse septa, but mostly (>65%) with only one septum at the base. it resembles monacrosporium indicum, m. sphaeroides and m. sinense, but can be distinguished from the first two species by lack of b ... | 2003 | 12967217 |
| towards a cost effective strategy for cutinase production by a recombinant saccharomyces cerevisiae: strain physiological aspects. | although the physiology and metabolism of the growth of yeast strains has been extensively studied, many questions remain unanswered where the induced production of a recombinant protein is concerned. this work addresses the production of a fusarium solani pisi cutinase by a recombinant saccharomyces cerevisiae strain induced through the use of a galactose promoter. the strain is able to metabolise the inducer, galactose, which is a much more expensive carbon source than glucose. both the transp ... | 2003 | 12658517 |
| purification and characterization of a cross-reactive 45-kd major allergen of fusarium solani. | fusarium solani (fs) is an important source of fungal allergen. a 45-kd major allergen of fs showed reactivity with patients' sera sensitive to many fungi. | 2003 | 12660423 |
| mycetoma of the foot caused by fusarium solani: identification of the etiologic agent by dna sequencing. | we report a case of fusarium solani mycetoma of the foot that could not be diagnosed by culture, but was correctly identified after amplification and sequence analysis of fungal internal transcribed spacers 1 and 2 and 5.8s ribosomal dna regions. | 2003 | 12682194 |
| integration of production and aqueous two-phase systems extraction of extracellular fusarium solani pisi cutinase fusion proteins. | genetic engineering was integrated with the production and purification of fusarium solani pisi cutinases, in order to obtain the highest amount of enzyme activity units, after purification. an aqueous two-phase system (atps) of polyethylene glycol 3350, dipotassium phosphate and whole broth was used for the extraction of three extracellular cutinases expressed in saccharomyces cerevisiae. the production/extraction process was evaluated regarding cutinases secretion in the medium, partition beha ... | 2003 | 12413786 |
| direct quantification of fungal dna from soil substrate using real-time pcr. | detection and quantification of genomic dna from two ecologically different fungi, the plant pathogen fusarium solani f. sp. phaseoli and the arbuscular mycorrhizal fungus glomus intraradices, was achieved from soil substrate. specific primers targeting a 362-bp fragment from the ssu rrna gene region of g. intraradices and a 562-bp fragment from the f. solani f. sp. phaseoli translation elongation factor 1 alpha gene were used in real-time polymerase chain reaction (pcr) assays conjugated with t ... | 2003 | 12609725 |
| tryptophan-tagged cutinase studied by steady state fluorescence for understanding of tag interactions in aqueous two-phase systems. | genetic engineering has been used to construct fusion proteins of fusarium solani pisi cutinase and tryptophan-based tags, expressed in saccharomyces cerevisiae, to increase the partitioning in aqueous two-phase systems. the separation systems were composed of thermoseparating polymers (random copolymers of ethylene oxide and propylene oxide, eopo) and detergents (c(12)eo(n)). in this study, the fluorescence behaviour of the peptide-tagged protein, free peptide tag and tryptophan was investigate ... | 2003 | 12637012 |
| a diffusible factor from arbuscular mycorrhizal fungi induces symbiosis-specific mtenod11 expression in roots of medicago truncatula. | using dual cultures of arbuscular mycorrhizal (am) fungi and medicago truncatula separated by a physical barrier, we demonstrate that hyphae from germinating spores produce a diffusible factor that is perceived by roots in the absence of direct physical contact. this am factor elicits expression of the nod factor-inducible gene mtenod11, visualized using a pmtenod11-gusa reporter. transgene induction occurs primarily in the root cortex, with expression stretching from the zone of root hair emerg ... | 2003 | 12644648 |