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failure to shorten the diagnostic delay in two ultra-orphan diseases (mucopolysaccharidosis types i and iii): potential causes and implications.rare diseases are often un- or misdiagnosed for extended periods, resulting in a long diagnostic delay that may significantly add to the burden of the disease. an early diagnosis is particularly essential if a disease-modifying treatment is available. the purpose of this study was to assess the extent of the diagnostic delay in the two ultra-rare diseases, i.e., mucopolysaccharidosis i (mps i) and iii (mps iii), both of which are lysosomal storage disorders with different phenotypic severities ( ...201829310675
simultaneous determination of six quality parameters of biodiesel through 1h nmr spectroscopy and partial least squares.biodiesel quality is checked by determining several parameters. considering the large number of analyses in this verification, as well as the disadvantages of the use of toxic solvents and waste generation, multivariate calibration is suggested to reduce the number of tests. in this work, hydrogen nuclear magnetic resonance (1h nmr) spectra were used to build multivariate models, from partial least squares (pls), in order to perform simultaneous determination of six important quality parameters ...201829310312
a simple and fast method to determine water content in biodiesel by electrochemical impedance spectroscopy.in this paper, a fast, simple and new alternative method for determination of water content (moisture) in biodiesel using electrochemical impedance spectroscopy (eis) is developed. the method is based on the sensitive variation of the charge transfer resistance (rct) of the biodiesel medium in the presence of different quantities of water. to obtain an accurate analytical measurement, a pre-treatment based on a simple dilution by acetonitrile is employed. the two identical pt electrodes are used ...201829310304
cai and sri molecules for iodine determination by high-resolution continuum source graphite furnace molecular absorption spectrometry: greener molecules for practical application.a new method to determine iodine in drug samples by high-resolution continuum source graphite furnace molecular absorption spectrometry (hr-cs gf mas) has been developed. the method measures the molecular absorption of a diatomic molecule, cai or sri (less toxic molecule-forming reagents), at 638.904 or 677.692nm, respectively, and uses a mixture containing 5μg of pd and 0.5μg of mg as chemical modifier. the method employs pyrolysis temperatures of 1000 and 800°c and vaporization temperatures of ...201829310275
gold leaf: from gilding to the fabrication of disposable, wearable and low-cost electrodes.gold is among the most used materials in electrocatalysis. despite this, this noble metal is still too expensive to be used in the fabrication of low cost and disposable devices. in the present work, gold-leaf sheets, usually employed in decorative crafts and wedding candies, is introduced as an inexpensive source of gold. planar-disc and nanoband gold electrodes were simply and easily manufactured by combining gold leaf and polyimide tape. the planar disc electrode exhibited electrochemical beh ...201829310267
real-time monitoring of a coffee roasting process with near infrared spectroscopy using multivariate statistical analysis: a feasibility study.this work proposes the use of near infrared (nir) spectroscopy in diffuse reflectance mode and multivariate statistical process control (mspc) based on principal component analysis (pca) for real-time monitoring of the coffee roasting process. the main objective was the development of a mspc methodology able to early detect disturbances to the roasting process resourcing to real-time acquisition of nir spectra. a total of fifteen roasting batches were defined according to an experimental design ...201829310234
exploiting multivariate calibration for compensation of iron interference in the spectrophotometric flow-based catalytic determination of molybdenum.multivariate calibration involving partial least squares was exploited in the flow-based spectrophotometric determination of molybdenum in river waters relying on the mo(vi)-catalyzed iodide oxidation by h2o2 under acidic conditions. two sample aliquots were simultaneously inserted into the carrier stream, and differential pumping was accountable for in-line addition of sulfuric acid to one of them. pronounced gradients (acidity and reagent concentrations) were established along the complex samp ...201829310215
a new and simple method for the simultaneous determination of amoxicillin and nimesulide using carbon black within a dihexadecylphosphate film as electrochemical sensor.the first electroanalytical method for the simultaneous determination of an important antibiotic (amoxicillin - amx) and an anti-inflammatory drug (nimesulide -nim), widely used in combination, is here proposed. in this method, a glassy carbon (gc) substrate modified with carbon black (cb) immobilized within a dihexadecylphosphate (dhp) film is used as electrochemical sensor (cb-dhp/gc). the electrochemical activity of this sensor was assessed (comparatively to that of gc) by electrochemical imp ...201829310210
targeting cytokines in reduction of depressive symptoms: a comprehensive review.heterogeneity in response to conventional antidepressants is a well-recognized limitation of evidence-based pharmacological treatments of major depressive disorder (mdd). abnormal activation of inflammatory pathways is postulated as one likely mechanism contributing to treatment resistance in mdd. in a subset of depressed patients, the balance between pro- and anti-inflammatory cytokines is thought to be altered, causing mood symptoms due to inflammation, as seen in co-morbid depression associat ...201829309829
combinations of laxatives and green banana biomass on the treatment of functional constipation in children and adolescents: a randomized study.evaluate the effect of combinations of green banana biomass and laxatives in children and adolescents with chronic constipation.201829309737
impaired systemic oxygen extraction in treated exercise pulmonary hypertension: a new engine in an old car?ambrisentan in 22 patients with pulmonary hypertension diagnosed during exercise (eph) improved pulmonary hemodynamics; however, there was only a trend toward increased maximum oxygen uptake (vo2max) secondary to decreased maximum exercise systemic oxygen extraction (ca-vo2). we speculate that improved pulmonary hemodynamics at maximum exercise "unmasked" a pre-existing skeletal muscle abnormality.201829309261
giant cell tumor of the frontal sinus: a typical finding in an unlikely location. 201829307940
burkitt-like lymphoma of the brain mimicking an intraventricular colloid cyst. 201829307939
retroperitoneal ewing's sarcoma/embryonal tumor: a rare differential diagnosis of back pain. 201829307936
fistula between the abdominal aorta and a retroaortic left renal vein: a rare complication of abdominal aortic aneurysm. 201829307934
common and uncommon features of focal splenic lesions on contrast-enhanced ultrasound: a pictorial review.the characterization of focal splenic lesions by ultrasound can be quite challenging. the recent introduction of contrast-enhanced ultrasound (ceus) has come to play a valuable role in the field of imaging splenic pathologies, offering the possibility of an ionizing radiation-free investigation. because ceus has been incorporated into everyday clinical practice, malignant diseases such as focal lymphomatous infiltration, metastatic deposits, benign cysts, traumatic fractures, and hemangiomas can ...201829307931
hepatocyte-specific contrast agent-enhanced magnetic resonance cholangiography: perioperative evaluation of the biliary tree.a large number of gadolinium chelates have recently been tested in clinical trials. some of those have already been approved for clinical use in the united states and europe. thus, new diagnostic perspectives have been incorporated into magnetic resonance imaging studies. among such gadolinium chelates are hepatobiliary-specific contrast agents (hscas), which, due to their property of being selectively taken up by hepatocytes and excreted by the biliary ducts, have been widely used for the detec ...201829307930
spect/ct with radiolabeled somatostatin analogues in the evaluation of systemic granulomatous infections.to evaluate spect/ct with radiolabeled somatostatin analogues (rsas) in systemic granulomatous infections in comparison with gallium-67 (67ga) citrate scintigraphy.201829307928
tomographic aspects of penetrating thoracic trauma: injuries from firearms and other weapons.the aim of this study was to analyze the various computed tomography findings in penetrating chest trauma, as well as to determine the frequency and extent of the lesions.201829307927
magnetic resonance imaging findings in central nervous system cryptococcosis: comparison between immunocompetent and immunocompromised patients.to assess the magnetic resonance imaging (mri) patterns associated with central nervous system infection with cryptococcus sp. in relation to patient immune status.201829307925
use of computed tomography and automated software for quantitative analysis of the vasculature of patients with pulmonary hypertension.to perform a quantitative analysis of the lung parenchyma and pulmonary vasculature of patients with pulmonary hypertension (ph) on computed tomography angiography (cta) images, using automated software.201829307924
development of a whole-cell-based screening method for a carotenoid assay using aerial microalgae.non-destructive approaches based on the application of optical spectroscopy are important for monitoring carotenoid accumulation in a whole cell cultured under various conditions. a simple and rapid assay utilizing aerial microalgae helps to identify stress conditions that can efficiently enhance the carotenogenesis in photosynthetic organisms. the spectra of cell suspensions were characterized in the aerial microalga coelastrella sp. kgu-y002, which are unicellular and undifferentiated. total c ...201829307837
antioxidant activity and associated structural attributes of halomicronema phycoerythrin.in the present study, blue light absorbing pigment protein phycoerythrin (pe) is purified up to molecular grade purity from marine halomicronema sp. r31dm. the purification method is based on the use of non-ionic detergent triton-x 100 in ammonium sulphate precipitation. the purified pe is characterized for its antioxidant activity in vitro and in vivo. pe is noted to show substantial in vitro antioxidant activity probed by various biochemical assays. the pe moderated rise in the intracellular-r ...201829307804
designing cross-linked xylanase aggregates for bioconversion of agroindustrial waste biomass towards potential production of nutraceuticals.immobilized biocatalysts design has the potential to efficiently produce valuable bioproducts from lignocellulosic biomass. among them, the carrier-free immobilization through the cross-linked enzyme aggregates technology is a simple and low-cost alternative. a two steps statistical approach was utilized to evaluate the synthesis of a cross-linked enzyme aggregate from a xylanolytic preparation, which was produced by cohnella sp. ar92 grown in a peptone-based culture medium. the resulting immobi ...201829307801
the u2af homology motif kinase 1 (uhmk1) is upregulated upon hematopoietic cell differentiation.uhmk1 (kis) is a nuclear serine/threonine kinase that possesses a u2af homology motif and phosphorylates and regulates the activity of the splicing factors sf1 and sf3b155. mutations in these components of the spliceosome machinery have been recently implicated in leukemogenesis. the fact that uhmk1 regulates these factors suggests that uhmk1 might be involved in rna processing and perhaps leukemogenesis. here we analyzed uhmk1 expression in normal hematopoietic and leukemic cells as well as its ...201829307747
acute periprosthetic joint infection due to fusobacterium nucleatum in a non-immunocompromised patient. failure using a debridement, antibiotics + implant retention approach.fusobacterium nucleatum is an obligately anaerobic gram-negative rod, a component of the microbiome of the oropharynx and the gastrointestinal and urogenital tracts, causing an array of human infections which often include periodontal pathologies. as far as we know, there are no previous publications about acute periprosthetic joint infection due to fusobacterium sp.; we report the first case in the medical literature of an aggressive, acute knee prosthetic infection due to f. nucleatum in a non ...201829307651
synthesis and evaluation of the mutagenicity of 3-alkylpyridine marine alkaloid analogues with anticancer potential.theonella sp is an important source of biologically-active 3-alkylpyridine alkaloids (3-apas) that has shown a wide variety of promising biological effects. in the present work, two new 3-apas analogues were synthesized based on molecular modeling studies to act as potential antimalarial agents. these theoneladin c analogues, containing the thiocyanate group in their chemical structures, were synthesized and evaluated against plasmodium falciparum (ic50 values ranging from 2.3 to 5.5μm). the str ...201829307373
evaluation of cognition and cortical excitability in huntington's disease.recent advances in neurophysiological techniques have contributed to our understanding of the pathophysiology of huntington's disease (hd). studies of the motor cortical excitability and central motor pathways have shown variable results.201829307324
investigation of the internal chemical composition of chitosan-based lbl films by depth-profiling x-ray photoelectron spectroscopy (xps) analysis.chitosan-based thin films were assembled using the layer-by-layer technique, and the axial composition was accessed using x-ray photoelectron spectroscopy with depth profiling. chitosan (chi) samples possessing different degrees of acetylation ([formula: see text]) and molecular weight ([formula: see text]) produced via the ultrasound-assisted deacetylation reaction were used in this study along with two different polyanions, namely, sodium polystyrenesulfonate (pss) and carboxymethylcellulose ( ...201829307187
upregulation of ghrelin gene expression in the excluded stomach of obese women with type 2 diabetes after roux-en-y gastric bypass in the surmetagit study.mechanisms of type 2 diabetes remission (t2dr) after roux-en-y gastric bypass (rygb) in obese patients appear to involve gastrointestinal hormones.201829307107
two new species of neanuridae (collembola: poduromorpha) from littoral of rio de janeiro, brazil.samples collected in "restinga" areas of two conservation units in rio de janeiro state revealed the presence of two new species of neanuridae family. the first, pseudachorutes solaris sp. nov., from parque estadual da costa do sol and from parque nacional da restinga de jurubatiba, differs from other species for having 34-50 vesicles in the postantennal organ, and the second, friesea jurubatiba sp. nov. from parque nacional da restinga de jurubatiba, differs from other species for having 8 clav ...201829307103
study of marine bacteria inactivation by photochemical processes: disinfection kinetics and growth modeling after treatment.the importance of seawater treatment in order to avoid microbiological pollution related to aquaculture or ballast water management has increased during the last few years. bacterial indicators used for the evaluation of different disinfection treatments are usually related with both waste and drinking water, these standards are not usual microorganisms found in seawater. thus, it is thought necessary to study the behavior of different marine-specific organisms in regard to improve the disinfect ...201829307073
aquaculture expansion in brazilian freshwaters against the aichi biodiversity targets.the convention on biological diversity proposed the aichi biodiversity targets to improve conservation policies and to balance economic development, social welfare, and the maintenance of biodiversity/ecosystem services. brazil is a signatory of the aichi biodiversity targets and is the most diverse country in terms of freshwater fish, but its national policies have supported the development of unsustainable commercial and ornamental aquaculture, which has led to serious disturbances to inland e ...201829306998
the effects of transcutaneous low-level laser therapy on the skin healing process: an experimental model.we aim to evaluate the action of transcutaneous laser in the initial wound healing process. the use of low-level laser therapy (lllt) has proven to be effective on inflammatory modulation and wound healing. the trial was performed on five groups of rats, through a dorsal incision. all groups received treatment on auricular artery. groups 1 and 3 were treated with transcutaneous lllt over a period of 15 min. groups 2 and 4 received one and two inactive laser applications (placebo), respectively. ...201829306974
patterns and predictors of skin score change in early diffuse systemic sclerosis from the european scleroderma observational study.our aim was to use the opportunity provided by the european scleroderma observational study to (1) identify and describe those patients with early diffuse cutaneous systemic sclerosis (dcssc) with progressive skin thickness, and (2) derive prediction models for progression over 12 months, to inform future randomised controlled trials (rcts).201829306872
an endophytic strain of methylobacterium sp. increases arsenate tolerance in acacia farnesiana (l.) willd: a proteomic approach. 201829306824
maternal sociodemographic factors differentially affect the risk of behavioral problems in brazilian and italian preterm toddlers.the main aim of the present study was to examine differences in behavioral problems in toddlers born very preterm in brazil and italy. the sample comprised 154 toddlers (18-24 months of age) born very preterm, including 76 toddlers from brazil and 78 toddlers from italy. the child behavior checklist was used to assess behavioral problems. specific sociodemographic factors (i.e., maternal age at childbirth and maternal education) and neonatal factors (i.e., gestational age, sex, and length of hos ...201829306797
high recycling efficiency and elemental sulfur purity achieved in a biofilm formed membrane filtration reactor.elemental sulfur (s0) is always produced during bio-denitrification and desulfurization process, but the s0 yield and purification quality are too low. till now, no feasible approach has been carried out to efficiently recover s0. in this study, we report the s0 generation and recovery by a newly designed, compact, biofilm formed membrane filtration reactor (bfmfr), where s0 was generated within a thauera sp. strain hdd-formed biofilm on membrane surface, and then timely separated from the biofi ...201829306789
identification and proposed relative and absolute configurations of niphimycins c-e from the marine-derived streptomyces sp. imb7-145 by genomic analysis.analysis of the whole genome sequence of streptomyces sp. imb7-145 revealed the presence of seven type i polyketide synthase biosynthetic gene clusters, one of which was highly homologous to the biosynthetic gene cluster of azalomycin f. detailed bioinformatic analysis of the modular organization of the pks gene suggested that this gene is responsible for niphimycin biosynthesis. guided by genomic analysis, a large-scale cultivation ultimately led to the discovery and characterization of four ne ...201829308897
lanthanide complexes of spiropyran photoswitch and sensor: spectroscopic investigations and computational modelling.a number of novel lanthanide (gd3+, sm3+, and tb3+) complexes of the 1',3'-dihydro-8-methoxy-1',3',3'-trimethyl-6-nitrospiro[2h-1-benzopyran-2,2'-(2h)-indole] (spiropyran; sp), a widely studied molecular photoswitch, were investigated. comparative spectroscopic (absorption and fluorescence) and kinetic investigations of the stimulated photochromic and solvatochromic behavior were carried out in different media. sp embedded in a rigid thin film of poly(methylmethacrylate) might be exploited profi ...201829308822
in vivo study of dermal collagen of striae distensae by confocal raman spectroscopy.this research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (sd) namely striae rubrae (sr) and striae albae (sa) when compared to normal skin (ns) using confocal raman spectroscopy. the methodology includes an in vivo human skin study for the comparison of confocal raman spectra of dermis region of sr, sa, and ns by supervised multivariate analysis using partial least squares discriminant analysis (pls-da) to determine qualitati ...201829308552
population genetic analysis reveals barriers and corridors for gene flow within and among riparian populations of a rare plant.landscape features and life-history traits affect gene flow, migration and drift to impact on spatial genetic structure of species. understanding this is important for managing genetic diversity of threatened species. this study assessed the spatial genetic structure of the rare riparian grevillea sp. cooljarloo (proteaceae), which is restricted to a 20 km2 region impacted by mining in the northern sandplains of the southwest australian floristic region, an international biodiversity hotspot. wi ...201829308125
natural hybridization and genetic and morphological variation between two epiphytic bromeliads.reproductive isolation is of fundamental importance for maintaining species boundaries in sympatry. here, we examine the genetic and morphological differences between two closely related bromeliad species: vriesea simplex and vriesea scalaris. furthermore, we examined the occurrence of natural hybridization and discuss the action of reproductive isolation barriers. nuclear genomic admixture suggests hybridization in sympatric populations, although interspecific gene flow is low among species in ...201829308124
high-resolution melting-curve (hrm) analysis for c. meleagridis identification in stool samples.cryptosporidiosis represents a major public health problem. this infection, caused by a protozoan parasite of the genus cryptosporidium, has been reported worldwide as a frequent cause of diarrhoea. in the immunocompetent host, the typical watery diarrhea can be self-limiting. however, it is severe and chronic, in the immunocompromised host and may cause death. cryptosporidium spp. are coccidians, which complete their life cycle in both humans and animals. the two species c. hominis and c. parvu ...201829306010
molecular discrimination of bacillus cereus group species in foods (lettuce, spinach, and kimbap) using quantitative real-time pcr targeting groel and gyrb.the aim of the study was to identify and evaluate specific biomarkers to differentiate within bacillus cereus group species from contaminated food samples with the use of real-time pcr. a total of 120 strains, comprising of 28 reference, 2 type, 78 wild strains of b. cereus and b. thuringiensis along with 12 strains representing 2 bacterial groups - b. mycoides, b. pseudomycoides, b. weihenstephanensis (b. cereus group); b. amyloliquefaciens, b. subtilis, enterococcus faecalis, escherichia coli, ...201829306007
discrimination of three siegesbeckiae herba species using uplc-qtof/ms-based metabolomics approach.the plant origin is one of the most important factors for the quality control of traditional chinese medicines (tcms) and highly affected on their safety and effectiveness in clinical applications. multi-origin has been widely observed for many tcms. siegesbeckiae herba (sh) is a traditional anti-rheumatic tcm which is originated from the plants of siegesbeckia pubescens makino (sp), s. orientalis l. (so), and s. glabrescens makino (sg). in the present study, an uplc-qtof/ms method were validate ...201829305931
the involvement of the pathway connecting the substantia nigra, the periaqueductal gray matter and the retrotrapezoid nucleus in breathing control in a rat model of parkinson's disease.parkinson's disease (pd) is characterized by a reduction in the number of dopaminergic neurons of the substantia nigra (snpc), accompanied by motor and non-motor deficiencies such as respiratory failure. here, our aim was to investigate possible neuronal communications between the snpc and chemoreceptor neurons within the retrotrapezoid nucleus (rtn), in order to explain neurodegeneration and the loss of breathing function in the 6-ohda pd animal model. male wistar rats received tracer injection ...201829305892
correction to: effects of exergaming on cardiovascular risk factors and adipokine levels in women.the original article was corrected.201829305789
microplastics in the benthic invertebrates from the coastal waters of kochi, southeastern arabian sea.this study examined microplastic particles present in the benthic invertebrates sternaspis scutata, magelona cinta (deposit feeders) and tellina sp. (suspension feeder) from the surface sediments of off-kochi, southwest coast of india. the microplastic particles and thread-like fibres detected in these organisms were identified to be polystyrene by using dxr raman microscope. examination of the microplastic particle in sternaspis scutata by epifluorescent microscopy showed fragmentation marks on ...201829305741
decorated superparamagnetic iron oxide nanoparticles with monoclonal antibody and diethylene-triamine-pentaacetic acid labeled with thechnetium-99m and galium-68 for breast cancer imaging.in this study we developed and tested an iron oxide nanoparticle conjugated with dtpa and trastuzumab, which can efficiently be radiolabeled with 99m-tc and ga-68, generating a nanoradiopharmaceutical agent to be used for spect and pet imaging.201829305666
comparative transcriptome analysis reveals the mechanism underlying 3,5-dibromo-4-hydroxybenzoate catabolism via a new oxidative-decarboxylation pathway.the compound 3,5-dibromo-4-hydroxybenzoate (dbhb) is both anthropogenically released into and naturally produced in the environment, and its environmental fate is of great concern. aerobic and anaerobic reductive dehalogenations are the only two reported pathways for dbhb catabolism. in this study, a new oxidative-decarboxylation pathway for dbhb catabolism was identified in a dbhb-utilizing strain of pigmentiphaga sp. h8. the genetic determinants underlying this pathway were elucidated based on ...201829305508
elucidating waterborne pathogen presence and aiding source apportionment in an impaired stream.fecal indicator bacteria (fib) are the basis for water quality regulations and are considered proxies for waterborne pathogens when conducting human health risk assessments. direct detection of pathogens in water and simultaneous identification of the source of fecal contamination is possible with microarrays, circumventing drawbacks to fib approaches. a multi-gene target microarray was used to assess the prevalence of waterborne pathogens in a fecally impaired, mixed use watershed. results indi ...201829305503
interaction of glossoscolex paulistus extracellular hemoglobin with hydrogen peroxide: formation and decay of ferryl-hbgp.the giant extracellular hemoglobin from earthworm glossoscolex paulistus (hbgp) reacts with hydrogen peroxide, displaying peroxidase activity in the presence of guaiacol. the formation of ferryl-hbgp (compound ii) from the peroxidase cycle was studied in the present work. the hypervalent ferryl-hbgp species was formed directly by the reaction of oxy-hbgp and hydrogen peroxide. the oxy-hbgp heme groups (144) under different excess of h2o2, relative to heme, showed an influence in the total amount ...201829305213
enhanced angiotensin ii induced sodium appetite in renovascular hypertensive rats.renovascular hypertensive 2-kidney, 1-clip (2k1c) rats have an increased activity of the renin-angiotensin system and an initial transitory increase in daily water and nacl intake. however, the dipsogenic and natriorexigenic responses to angiotensin ii (ang ii) have not been tested yet in 2k1c rats. therefore, in the present study, we evaluated water and 0.3 m nacl intake induced by water deprivation (wd)-partial rehydration (pr) or intracerebroventricular (icv) ang ii in 2k1c rats. in addition, ...201829305157
the waterbirth project: são bernardo hospital experience.the following quantitative observational study aimed to analyse the maternal and neonatal outcomes of 90 low-risk pregnant women who gave birth in water at são bernardo hospital.201829305115
referral gynecological ambulatory clinic: principal diagnosis and distribution in health services.the association between gynecological diagnoses and their distribution in the health sectors provides benefits in the field of women's health promotion and in medical and interdisciplinary education, along with rationalization according to level of care complexity. thus, the objective is analyze the clinical-demographic characteristics, main diagnoses in gynecological ambulatory care, and their distribution in health services.201829304796
extraction and partial characterisation of antioxidant pigment produced by chryseobacterium sp. kr6.pigments synthesised by chryseobacterium sp. kr6 growing on feather waste were extracted and characterised. the pigment extract was characterised by koh test, uv-vis, cielab colour system, hplc-dad-ms, ftir and its antioxidant capacity was evaluated. a positive bathochromic shift was observed when kr6 colonies or pigment extracts were subjected to alkaline solution (20% koh) and a λmax at 450 nm was detected for acetone extracts, although no typical fine structure of carotenoids was detected in ...201829304560
zn2+ induced molecular responses associated with oxidative stress, dna damage and histopathological lesions in liver and kidney of the fish, channa punctatus (bloch, 1793).zn2+ is essential for normal physiological functioning of all organisms in small quantities, but when its concentration enhances in surrounding environment it acts as a toxicant to organisms. common sources of zn2+ pollution are electroplating, alloying, mining, and allied industrial operations. the present study aims to assess the biochemical, histopathological and genotoxicological implications under zn2+ intoxication along with its accumulation patterns in prime biotransformation sites-liver ...201829304413
photosynthetic production of sunscreen shinorine using an engineered cyanobacterium.mycosporine-like amino acids (maas) are secondary metabolites of a variety of marine organisms including cyanobacteria and macroalgae. these compounds have strong ultraviolet (uv) absorption maxima between 310 and 362 nm and are biological sunscreens for counteracting the damaging effects of uv radiation in nature. the common maa shinorine has been used as one key active ingredient of environmentally friendly sunscreen creams. commercially used shinorine is isolated from one red algae that is ge ...201829304277
local chatter or international buzz? language differences on posts about zika research on twitter and facebook.when the zika virus outbreak became a global health emergency in early 2016, the scientific community responded with an increased output of zika-related research. this upsurge in research naturally made its way into academic journals along with editorials, news, and reports. however, it is not yet known how or whether these scholarly communications were distributed to the populations most affected by zika.201829304110
combined evaluation of sexually transmitted infections in hiv-infected pregnant women and infant hiv transmission.sexually transmitted infections (stis) including chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), treponema pallidum (tp), and cytomegalovirus (cmv) may lead to adverse pregnancy and infant outcomes. the role of combined maternal stis in hiv mother-to-child transmission (mtct) was evaluated in mother-infant pairs from nichd hptn 040.201829304083
functional expression and characterization of the recombinant n-acetyl-glucosamine/n-acetyl-galactosamine-specific marine algal lectin bpl3.lectins, characterized by their carbohydrate-binding ability, have extensive practical applications. however, their industrial use is limited due to impurity. thus, quality-controlled production of recombinant lectin is necessary. in this study, the algal lectin bpl3 (bryopsis plumosa lectin 3) was successfully produced using a bacterial expression system, bl21(de3), with an artificial repeated structure (dimeric construct). recombinant dimeric bpl3 (rd2bpl3) was confirmed by lc-ms/ms spectromet ...201829303968
review of "readiness of us general surgery residents for independent practice" by george bc, bohnen jd, williams rg, meyerson sl, schuller mc, clark mj, meier ah, torbeck l, mandell sp, mullen jt, smink ds, scully re, chipman jg, auyang ed, terhune kp, wise pe, choi jn, foley ef, dimick jb, choti ma, soper nj, lillemoe kd, zwischenberger jb, dunnington gl, darosa da, fryer jp; procedural learning and safety collaborative (plsc) in ann surg 266: 582-594, 2017. 201829303855
sphingobacterium terrae sp. nov., isolated from oil-contaminated soil.a cream-coloured, gram-stain-negative, non-motile and rod-shaped bacterium, designated strain brt-ft, was isolated from oil-contaminated soil. strain brt-ft was able to grow from 15 to 45 °c, ph 6.5-10.5 and 0-4.5 % (w/v) nacl concentration. this strain was taxonomically characterized by a polyphasic approach. based on 16s rrna gene sequence analysis, strain brt-ft represented a member of the genus sphingobacterium and shared highest sequence similarity with sphingobacterium chuzhouense dh-5t (9 ...201829303695
marinobacterium aestuarii sp. nov., a benzene-degrading marine bacterium isolated from estuary sediment.a gram-stain-negative, aerobic, motile, flagellated rod-shaped bacterium, designated st58-10t, was isolated from an estuarine sediment in the republic of korea. the strain was able to degrade benzene. growth of strain st58-10t was observed at 4-35 °c (optimum, 20-25 °c), ph 5-9 (optimum, ph 7-8) and 1-8 % nacl (optimum, 3 %). phylogenetic analyses based on 16s rrna gene sequences showed that strain st58-10t formed a phyletic lineage within the genus marinobacterium of the family oceanospirillace ...201829303694
polaribacter staleyi sp. nov., a polysaccharide-degrading marine bacterium isolated from the red alga ahnfeltia tobuchiensis.a gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10alg 139t, was isolated from the pacific red alga ahnfeltiato buchiensis. the phylogenetic analysis based on 16s rrna gene sequences showed that the novel strain belonged to the genus polaribacter, a member of the family flavobacteriaceae, the phylum bacteroidetes, with highest sequence similarity to polaribacter butkevichii kmm 3938t (99.3 %) and 93.3-98.6 % to other recognized polaribacter s ...201829303693
characterization of novel gene yr79 and four additional qtl for all-stage and high-temperature adult-plant resistance to stripe rust in spring wheat pi 182103.stripe rust, caused by puccinia striiformis f. sp. tritici, is an important disease of wheat worldwide. exploring new resistance genes is essential for breeding resistant wheat cultivars. pi 182103, a spring wheat landrace originally from pakistan, has shown a high level of resistance to stripe rust in fields for many years, but genes for resistance to stripe rust in the variety have not been studied. to map the resistance gene(s) in pi 182103, 185 recombinant inbred lines (rils) were developed ...201829303685
an optical biosensor-based quantification of the microcystin synthetase a gene: early warning of toxic cyanobacterial blooming.the monitoring and control of toxic cyanobacterial strains, which can produce microcystins, is critical to protect human and ecological health. we herein reported an optical-biosensor-based quantification of the microcystin synthetase a (mcya) gene so as to discriminate microcystin-producing strains from nonproducing strains. in this assay, the mcya-specific ssdna probes were designed in silico with an on-line tool and then synthesized to be covalently immobilized on an optical-fiber surface. pr ...201829303555
palladium-catalyzed enantioselective c(sp2)-h arylation of ferrocenyl ketones enabled by a chiral transient directing group.palladium-catalyzed enantioselective c(sp2)-h activation of ferrocenyl ketones is achieved through utilizing catalytic, inexpensive l-tert-leucine as a chiral transient directing group. the transformation allows rapid access to ferrocene scaffolds simultaneously possessing planar- and stereogenic central chirality, widely applied in the ferrocene-based chiral ligand families.201829303517
improving the induction fold of riboregulators for cyanobacteria.cyanobacteria are ideal cellular factories for biochemical production because of their ability to fix co2 by photosynthesis and convert this molecule into biochemicals. previously, we engineered a riboregulator that enables post-transcriptional gene regulation in the cyanobacterium synechocystis sp. pcc 6803. here, we improved the riboregulator by designing two rna species, tarna and crrna, to enhance its induction fold. we inserted nucleotides into the crrna loop to enhance intermolecular hybri ...201829303421
application of plasma sprayed zirconia coating in dental implant: study in implant.the aim was to investigate the osseointegration of a novel coating-plasma-sprayed nanostructured zirconia (nsz) in dental implant. nanostructured zirconia coating on non-thread titanium implant was prepared by plasma spraying, the implant surface morphology, surface roughness and wettability were measured. in vivo, nanostructured zirconia-coated implants were inserted in rabbit tibia and animals were respectively sacrificed at 2, 4, 8 and 12 weeks after implantation. the bond strength between im ...201829303416
youth engagement in pediatric rehabilitation: service providers' perceptions in a real-time study of solution-focused coaching for participation goals.client engagement is assumed to affect therapy outcomes. this study examined service providers' perceptions of youth engagement in solution-focused coaching sessions focusing on participation-oriented goals for youth with cerebral palsy.201829303397
serpulanines a to c, n-oxidized tyrosine derivatives isolated from the sri lankan fungus serpula sp.: structure elucidation, synthesis, and histone deacetylase inhibition.serpulanines a (1), b (2), and c (3) have been isolated from extracts of the rare sri lankan macrofungus serpula sp. the structures of 1, 2, and 3 were elucidated by a combination of spectroscopic and single-crystal x-ray diffraction analyses. serpulanines a (1) and b (2) both contain the rare (e)-2-hydroxyimino hydroxamic acid functional group array. a proposed biogenesis for serpulanine b (2) suggests that its (e)-2-hydroxyimino hydroxamic acid moiety arises from a diketopiperazine precursor. ...201829303267
andro-ivf: a novel protocol for poor responders to ivf controlled ovarian stimulation.this study aimed to assess a novel protocol designed to improve poor ovarian response through intra-ovarian androgenization. the endpoints were: number of oocytes and mature oocytes retrieved, fertilization, cancellation and pregnancy rates.201829303236
propolis increases neutrophils response against candida albicans through the increase of reactive oxygen species.to investigate whether brazilian green propolis improves the immune response against recurrent form isolate recurrent vulvovaginal candidiasis (rvvc) caused by candida albicans by increasing neutrophil oxidative burst.201829302986
biodegradation of sulfonamides by shewanella oneidensis mr-1 and shewanella sp. strain mr-4.because of extensive sulfonamides application in aquaculture and animal husbandry and the consequent increase in sulfonamides discharged into the environment, strategies to remediate sulfonamide-contaminated environments are essential. in this study, the resistance of shewanella oneidensis mr-1 and shewanella sp. strain mr-4 to the sulfonamides sulfapyridine (spy) and sulfamethoxazole (smx) were determined, and sulfonamides degradation by these strains was assessed. shewanella oneidensis mr-1 an ...201829302823
mitochondrial bioenergy alterations in avian hd11 macrophages infected with infectious bronchitis virus.to establish an association between mitochondrial dysfunction and apoptosis following infectious bronchitis virus (ibv) infection, hd11 avian macrophage cells were infected with the massachusetts 41 (m41) strain. our results show that the m41 strain of ibv induced cytopathic effects followed by the release of new viral particles. elevated numbers of apoptotic cells were observed at 24, 48 and 72 h post-infection (p.i.). viral infection was associated with mitochondrial membrane depolarization an ...201829302792
pyramiding of transgenic pm3 alleles in wheat results in improved powdery mildew resistance in the field.the combined effects of enhanced total transgene expression level and allele-specificity combination in transgenic allele-pyramided pm3 wheat lines result in improved powdery mildew field resistance without negative pleiotropic effects. allelic pm3 resistance genes of wheat confer race-specific resistance to powdery mildew (blumeria graminis f. sp. tritici, bgt) and encode nucleotide-binding domain, leucine-rich repeat (nlr) receptors. transgenic wheat lines overexpressing alleles pm3a, b, c, d, ...201829302719
genome sequence of the bile salt-degrading bacterium novosphingobium sp. strain chol11, a model organism for bacterial steroid catabolism.many bacteria from different phylogenetic groups are able to degrade eukaryotic steroid compounds, but the underlying metabolic pathways are still not well understood. novosphingobium sp. strain chol11 is a steroid-degrading alphaproteobacterium. its genome sequence reveals that it lacks several genes for steroid degradation known to exist in other model organisms.201829301902
draft genome sequence of a red-pigmented janthinobacterium sp. native to the hudson valley watershed.water samples from the hudson valley watershed indicate that the area is host to many violacein-producing bacterial isolates. here, we report the draft whole-genome sequence of janthinobacterium sp. strain bjb412, an isolate lacking violacein production yet containing genes responsible for prodigiosin, biofilm production, and quorum sensing, like its purple-pigmented counterparts.201829301893
draft genome sequence of a violacein-producing iodobacter sp. from the hudson valley watershed.iodobacter species are among a number of freshwater gram-negative violacein-producing bacteria. janthinobacterium lividum and chromobacterium violaceum have had their whole genomes sequenced and annotated. this is the first report of a draft whole-genome sequence of a violacein-producing iodobacter strain that was isolated from the hudson valley watershed.201829301892
draft genome sequence of rhodococcus sp. strain ncimb 12038, a naphthalene-degrading bacterium.we report here the draft genome sequence of rhodococcus sp. strain ncimb 12038, an industrially important bacterium, possessing a large and diverse repertoire of genes involved in the biotransformation of various organic compounds, including naphthalene.201829301890
draft genome sequence of the predatory marine bacterium halobacteriovorax sp. strain jy17.a draft genome sequence of halobacteriovorax sp. strain jy17 was assembled from a metagenomic data set. the 3.47-mbp genome of this unusual predatory bacterium contains 3,263 protein-coding sequences, 33 trnas, and 2 copies each of the 16s, 23s, and 5s rrna genes. this is only the third sequenced representative of this genus.201829301887
draft genome sequence of marinobacter sp. strain ant_b65, isolated from antarctic marine sponge.marinobacter sp. strain ant_b65 was isolated from sponge collected in king george island, antarctica. the draft genome of 4,173,840 bp encodes 3,743 protein-coding open reading frames. the genome will provide insights into the strain's potential use in the production of natural products.201829301884
draft genome sequence of streptomyces sp. strain jv178, a producer of clifednamide-type polycyclic tetramate macrolactams.here, we report the draft genome sequence of streptomyces sp. jv178, a strain originating from connecticut (usa) garden soil. this strain produces the polycyclic tetramate macrolactam compounds clifednamides a and b. the draft genome contains 10.65 mb, 9,045 predicted protein coding sequences, and several natural product biosynthetic loci.201829301882
complete genome sequence of photobacterium leiognathi strain js01.photobacterium leiognathi is a bioluminescent symbiont of fish of the leiognathidae family. here, we present the full-genome sequence of p. leiognathi strain js01, a strain isolated from a nonluminescent loligo sp. squid of singaporean origin. no finished genome sequence of this species is currently publicly available.201829301879
characterization of newly isolated pseudonocardia sp. n23 with high 1,4-dioxane-degrading ability.this study was conducted to elucidate the 1,4-dioxane degradation characteristics of a newly isolated 1,4-dioxane-degrading bacterial strain and evaluate the applicability of the strain to biological 1,4-dioxane removal from wastewater. a bacterial strain (designated strain n23) capable of degrading 1,4-dioxane as the sole carbon and energy source was isolated from an enrichment culture prepared from 1,4-dioxane-contaminated groundwater. strain n23 was phylogenetically identified as belonging to ...201829301721
quality of life scores differs between genotypic groups of patients with suspected hereditary hemochromatosis.hereditary hemochromatosis (hh) encompasses a group of autosomal recessive disorders mainly characterized by enhanced intestinal absorption of iron and its accumulation in parenchymal organs. hh diagnosis is based on iron biochemical and magnetic resonance imaging (mri) assessment, and genetic testing. questionnaires, such as sf-36 (short form health survey), have been increasingly used to assess the impact of diseases on the patient's quality of life (ql). in addition, different genotypes are i ...201829301508
helminth parasites of the lizard nothobachia ablephara (gymnophthalmidae) in caatinga areas from neotropical semiarid.the lizard nothobachia ablephara is endemic to dune areas and sandy soils adjacent to the são francisco river in semiarid northeastern brazil. forty-nine lizard specimens were collected in 2 caatinga areas in the municipality of petrolina, in pernambuco state. three gastrointestinal helminth taxa were identified, the nematodes parapharyngodon alvarengai and physaloptera sp., and the cestode oochoristica sp. nothobachia ablephara showed low parasite richness, however higher levels of infection by ...201829301442
sistematx, an online web-based cheminformatics tool for data management of secondary metabolites.the traditional work of a natural products researcher consists in large part of time-consuming experimental work, collecting biota to prepare and analyze extracts and to identify innovative metabolites. however, along this long scientific path, much information is lost or restricted to a specific niche. the large amounts of data already produced and the science of metabolomics reveal new questions: are these compounds known or new? how fast can this information be obtained? to answer these and o ...201829301376
beneficial effects of monascus sp. kccm 10093 pigments and derivatives: a mini review.the production of monascus pigments and related byproducts, via microbial fermentation, has been broadly utilized as coloring by traditional food industries and as a natural textile dye. in addition to these traditional purposes, monascus pigments have been recently favored for a variety of commercial and academic purposes. pigments and derivatives formed during monascus fermentation have pharmaceutical and clinical properties that can counteract common diseases, including obesity, type-2 diabet ...201829301350
eicosanoid diversity of stony corals.oxylipins are well-established lipid mediators in plants and animals. in mammals, arachidonic acid (aa)-derived eicosanoids control inflammation, fever, blood coagulation, pain perception and labor, and, accordingly, are used as drugs, while lipoxygenases (lox), as well as cyclooxygenases (cox) serve as therapeutic targets for drug development. in soft corals, eicosanoids are synthesized on demand from aa by lox, cox, and catalase-related allene oxide synthase-lipoxygenase (caos-lox) and hydrope ...201829301345
expression and activity of cox-1 and cox-2 in acanthamoeba sp.-infected lungs according to the host immunological status.little is known about the pathomechanism of pulmonary infections caused by acanthamoeba sp. therefore, the aim of this study was to determine whether acanthamoeba sp. may affect the expression and activity of cyclooxygenase-1 (cox-1) and cyclooxygenase-2 (cox-2), resulting in the altered levels of their main products, prostaglandins (pge₂) and thromboxane b₂ (txb₂), in lungs of immunocompetent or immunosuppressed hosts. acanthamoeba sp. induced a strong expression of cox-1 and cox-2 proteins in ...201829301283
management of minor ailments in a community pharmacy setting: findings from simulated visits and qualitative study in gondar town, ethiopia.community pharmacy professionals are being widely accepted as sources of treatment and advice for managing minor ailments, largely owing to their location at the heart of the community. the aim of the present study was, therefore, to document the involvement of community pharmacy professionals in the management of minor ailments and perceived barriers that limit their provision of such services. simulated patient (sp) visits combined with a qualitative study using in-depth interviews was conduct ...201829300785
a kolmogorov-smirnov test for the molecular clock based on bayesian ensembles of phylogenies.divergence date estimates are central to understand evolutionary processes and depend, in the case of molecular phylogenies, on tests of molecular clocks. here we propose two non-parametric tests of strict and relaxed molecular clocks built upon a framework that uses the empirical cumulative distribution (ecd) of branch lengths obtained from an ensemble of bayesian trees and well known non-parametric (one-sample and two-sample) kolmogorov-smirnov (ks) goodness-of-fit test. in the strict clock ca ...201829300759
the my meal intake tool (m-mit): validity of a patient self- assessment for food and fluid intake at a single meal.hospital malnutrition is an under-recognized issue that leads to a variety of adverse outcomes, especially for older adults. food/fluid intake (ffi) monitoring in hospital can be used to identify those who are improving and those who need further treatment. current monitoring practices such as calorie counts are impractical for all patients and a patient-completed tool, if valid, could support routine ffi monitoring. the aim of this research was to determine whether the patient-completed my meal ...201829300419
larkinella knui sp. nov., isolated from soil.a gram-stain-negative, motile by gliding, rod-shaped, aerobic bacterium, designated 15j6-3t6t, was isolated from a soil sample collected from jeju island, south korea, and characterized taxonomically using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain 15j6-3t6t belongs to the family cytophagaceae and is related to larkinella harenae 15j9-9t (93.9 % similarity), larkinella arboricola z0532t (93.6 %), larkinella bovis m2tb15t (93.3 %), and larkinella inspera ...201829300161
planococcus salinus sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.a novel aerobic, gram-stain-positive, motile, moderately halophilic and coccoid bacterial strain, designated lcb217t, was isolated from a saline-alkali soil in north-western china and identified using a polyphasic taxonomic approach. growth occurred with 3-15 % (w/v) nacl (optimum 3-5 %), at 10-45 °c (optimum 30 °c) and at ph 7.0-9.0 (optimum ph 9.0). strain lcb217t contained mk-7 and mk-8 as the predominant menaquinones and anteiso-c15 : 0, iso-c14 : 0 and iso-c16 : 0 as the major fatty acids. ...201829300160
lactococcus reticulitermitis sp. nov., isolated from the gut of the subterranean termite reticulitermes speratus.strain rs-y01t was isolated from the gut of the wood-feeding subterranean termite reticulitermes speratus. phylogenetic analysis based on 16s rrna gene sequence indicated that the strain rs-y01t belonged to the genus lactococcus and was most closely related to lactococcus raffinolactis jcm 5706t with 98.1 % similarity in the 16s rrna gene, followed by lactococcus piscium jcm 16647t (97.2 %). genomic comparisons of strain rs-y01t with l. raffinolactis jcm 5706twere made using the genome-to-genome ...201829300157
bifidobacterium catulorum sp. nov., a novel taxon from the faeces of the baby common marmoset (callithrix jacchus).in our previous study based on hsp60 pcr-restriction fragment length polymorphism and 16s rrna gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus bifidobacterium. in that study, we also proposed that these isolated groups potentially represented novel species of the genus bifidobacterium. out of them, bifidobacterium aesculapii, bifidobacterium my ...201829300153
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