Publications

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radioimmunoassay analysis of baculovirus granulins and polyhedrins.granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis from the baculoviruses autographa californica, rachiplusia ou, heliothis zea, heliothis armigera, trichoplusia ni, and spodoptera frugiperda. antisera were raised against autographa californica (ac) polyhedrin and trichoplusia ni (tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (ria). ac polyhedrin and tn ...198018631650
the multicomponent genome of a different cytoplasmic polyhedrosis virus isolated from heliothis armigera.the genome of a new cytoplasmic polyhedrosis virus (cpv) isolated from heliothis armigera has been shown to consist of 10 double-stranded rna segments, as in other cpvs, but the genome profile on page bears no resemblance to previously described cpvs isolated from h.armigera.1978361635
isolation of a cytoplasmic polyhedrosis virus by physical and immunological techniques.procedures are described for the separation and purification of cytoplasmic polyhedrosis viruses obtained from multiply infected heliothis armigera larvae. separation was achieved by differential centrifugation and density gradient zone electrophoresis followed by complexing with nuclear polyhedrosis virus specific antibody. the yield of cytoplasmic polyhedrosis virus was increased by passage in larvae reared on synthetic media.19751098037
bioassay of a nuclear-polyhedrosis virus and bacillus thuringiensis against the american bollworm, heliothis armigera, in botswana. 19744597764
weight-related susceptibility of larvae of heliothis armigera to a crude nuclear-polyhedrosis virus preparation. 19744597767
hexokinase is a key regulator of energy metabolism and ros activity in insect lifespan extension.developmental arrest (diapause) is a 'non-aging' state that is similar to the caenorhabditis elegans dauer stage and drosophila lifespan extension. diapause results in low metabolic activity and a profound extension of insect lifespan. here, we cloned the helicoverpa armigera hexokinase (hk) gene, a gene that is critical for the developmental arrest of this species. hk expression and activity levels were significantly increased in nondiapause-destined pupae compared with those of diapause-destin ...026852422
identification, characterization, and expression of a novel p450 gene encoding cyp6ae25 from the asian corn borer, ostrinia furnacalis.an allele of the cytochrome p450 gene, cyp6ae14, named cyp6ae25 (genbank accession no. eu807990) was isolated from the asian com borer, ostrinia fumacalis (guenée) (lepidoptera: pyralidae) by rt-pcr. the cdna sequence of cyp6ae25 is 2315 bp in length and contains a 1569 nucleotides open reading frame encoding a putative protein with 523 amino acid residues and a predicted molecular weight of 59.95 kda and a theoretical pi of 8.31. the putative protein contains the classic heme-binding sequence m ...021529257
development associated profiling of chitinase and microrna of helicoverpa armigera identified chitinase repressive microrna.expression of chitinase is developmentally regulated in insects in consonance with their molting process. during the larval-larval metamorphosis in helicoverpa armigera, chitinase gene expression varies from high to negligible. in the five-day metamorphic course of fifth-instar larvae, chitinase transcript is least abundant on third day and maximal on fifth day. microrna library prepared from these highest and lowest chitinase-expressing larval stages resulted in isolation of several mirnas. in ...023887392
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