Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
culture proven bacterial meningitis in children: agents, clinical profile and outcome.meningitis is a serious infection. little is known about the bacterial agents and their antibacterial sensitivity in nepalese children.201322610807
epidemiology of seasonal influenza in bangkok between 2009 and 2012.this study investigated influenza activity in bangkok, thailand between june 2009 and july 2012.201324129626
[monitoring of viral pathogens in pediatric intensive care unit and analysis of clinical significance].to study the characteristics of viral spectrum and clinical features of children in pediatric intensive care unit (picu).201324131585
respiratory virus detection in nasopharyngeal aspirate versus bronchoalveolar lavage is dependent on virus type in children with chronic respiratory symptoms.the comparative yield of respiratory virus detection from nasopharyngeal aspirate (npa) versus bronchoalveolar lavage (bal) is uncertain. furthermore, the significance of virus detection and its relationship to lower airway neutrophilic inflammation is poorly studied.201324125830
[virus detection in bronchoalveolar lavage fluid of 122 children with severe pneumonia].to investigate the prevalence of viral pathogen in children with severe pneumonia in hunan.201324044210
burden of respiratory tract infections among paediatric in and out-patient units during 2010-11.respiratory tract infections due to viral etiology were studied with an objective to identify and compare the pathogens between hospital indoor and outdoor units.201323609364
identification of respiratory viruses with a novel point-of-care multianalyte antigen detection test in children with acute respiratory tract infection.rapid etiological diagnosis of a respiratory virus infection may have impact on antiviral and antibiotic therapy, patient cohorting, and prediction of the clinical course. most point-of-care tests for detection of respiratory viruses have limitations in diagnostic performance and clinical usability. a novel, multianalyte point-of-care antigen detection test system (maripoc(®); arcdia international oy ltd., turku, finland) detects eight respiratory viruses (influenza a and b viruses, respiratory ...201323490399
impact of viral infections in children with community-acquired pneumonia: results of a study of 17 respiratory viruses.little is known about the prevalence of viral infections in children with community-acquired pneumonia (cap).201322329841
association between human parainfluenza virus type 1 and smoking history in patients with an abdominal aortic aneurysm.several studies have suggested that infectious agents may induce the development of abdominal aortic aneurysms and/or accelerate their progression. the aim of this study was to evaluate the presence of the respiratory-transmitted viruses such as influenza a and b and parainfluenza type 1 genomes in bioptic fragments of abdominal aortic aneurysms. furthermore, the association between viral infection and traditional risk factors for aneurysms was investigated employing multivariate logistic regres ...201323097301
evaluation of a novel real-time rt-pcr using toce technology compared with culture and seeplex rv15 for simultaneous detection of respiratory viruses.various kinds of commercial molecular systems have been developed for fast and more accurate detection of respiratory viruses. anyplex™ ii rv16 [rv16] was designed for simultaneous detection of 16 respiratory viruses using multiplex pcr coupled with toce™ technology.201323743345
uncommon cause of severe pneumonia: co-infection of influenza b and streptococcus.influenza and pneumococcus co-infection can cause severe morbidity and mortality. usually, this entails influenza a, while infection by influenza b is rarely serious. the literature describes influenza a epidemics leading to prolific loss of lives, notably the 1918 epidemic was blamed for the deaths of 40 to 50 million people. in this report, four patients were infected by influenza b during the influenza epidemic of 2011/12 in hong kong. all of them were previously healthy and had no chronic di ...201324310663
spectrum of respiratory viruses circulating in eastern india: prospective surveillance among patients with influenza-like illness during 2010-2011.in developing countries, viruses causing respiratory disease are a major concern of public health. during january 2010-december 2011, 2,737 patients with acute respiratory infection from the outpatient departments as well as patients admitted to hospitals were screened for different respiratory viruses. nasal and or throat swabs were collected and transported to the laboratory where initial screening of influenza a and influenza b viruses was performed. the samples were tested further for influe ...201323765782
[comparison of clinical features and co-infection between pneumonia caused by influenza virus a and pneumonia caused by influenza virus b among children].to compare the clinical features and co-infection between pneumonia caused by influenza virus a (iva) and pneumonia caused by influenza virus b (ivb) among children.201324229596
cerebellar mutism and reversible cytotoxic edema in influenza b-associated encephalopathy.influenza virus-associated neurological complications are rare, though well-documented, especially in children. encephalopathy and seizures are the most common complications and are typically associated with influenza a infection. cerebellar mutism has been rarely reported in association with influenza b infection.201324095574
influenza b-induced refractory cardiogenic shock: a case report.an association between influenza a viruses and myocarditis was noted during the 1918 influenza pandemic. since then, the link between the influenza b virus and fulminant myocarditis or cardiogenic shock has been rarely reported.201324079537
structural basis for the divergent evolution of influenza b virus hemagglutinin.influenza a and b viruses are responsible for the severe morbidity and mortality worldwide in annual influenza epidemics. currently circulating influenza b virus belongs to the b/victoria or b/yamagata lineage that was diverged from each other about 30-40 years ago. however, a mechanistic understanding of their divergent evolution is still lacking. here we report the crystal structures of influenza b/yamanashi/166/1998 hemagglutinin (ha) belonging to b/yamagata lineage and its complex with the a ...201324074573
improved sensitivity of the novel xpert flu test for detection of influenza b virus. 201324068015
influenza b infection complicated by patent foramen ovale and high altitude pulmonary edema.a 34-year-old man presented to a clinic at a ski resort in the rocky mountains at 9000 feet (2743 m) with shortness of breath and fatigue, a few days after arriving to altitude from sea level. he was found to be hypoxic with radiographic findings consistent with high altitude pulmonary edema (hape). he was treated with high flow oxygen, steroids, and calcium channel blockers and transferred to a lower altitude tertiary care hospital for intensive care unit monitoring and further treatment. durin ...201323022059
differing epidemiological dynamics of influenza b virus lineages in guangzhou, southern china, 2009-2010.the epidemiological and evolutionary dynamics of the two cocirculating lineages of influenza b virus, victoria and yamagata, are poorly understood, especially in tropical or subtropical areas of southeast asia. we performed a phylogenetic analysis of the hemagglutinin (ha) and neuraminidase (na) sequences of influenza b viruses isolated in guangzhou, a southern chinese city, during 2009 to 2010 and compared the demographic and clinical features of infected patients. we identified multiple viral ...201324027322
molecular characterization of the receptor binding structure-activity relationships of influenza b virus hemagglutinin.selectivity of α2,6-linked human-like receptors by b hemagglutinin (ha) is yet to be fully understood. this study integrates binding data with structure-recognition models to examine the impact of regional-specific sequence variations within the receptor-binding pocket on selectivity and structure activity relationships (sar). the receptor-binding selectivity of influenza b has corresponding to either b/victoria/2/1987 or the b/yamagata/16/88 lineages was examined using surface plasmon resonance ...201324020757
neuraminidase inhibitors for influenza b virus infection: efficacy and resistance.many aspects of the biology and epidemiology of influenza b viruses are far less studied than for influenza a viruses, and one of these aspects is efficacy and resistance to the clinically available antiviral drugs, the neuraminidase (na) inhibitors (nais). acute respiratory infections are one of the leading causes of death in children and adults, and influenza is among the few respiratory infections that can be prevented and treated by vaccination and antiviral treatment. recent data has sugges ...201324013000
the specificity of the influenza b virus hemagglutinin receptor binding pocket: what does it bind to?the influenza surface glycoprotein hemagglutinin (ha) binds to sialylglycoproteins and sialylglycolipids on the surface of host cells. these sialyl-glycans, usually linked to galactose in either α2,6 or α2,3 configurations, are the receptors for the viral ha, the binding to which promotes viral attachment, membrane fusion, and internalization of the virus. this review examines all of the available receptor binding data on the influenza b ha and provides a structure recognition perspective for th ...201323996486
sequence-specific cleavage of bm2 gene transcript of influenza b virus by 10-23 catalytic motif containing dna enzymes significantly inhibits viral rna translation and replication.one of the hallmarks of progression of influenza virus replication is the step involving the virus uncoating that occurs in the host cytoplasm. the bm2 ion channel protein of influenza b virus is highly conserved and is essentially required during the uncoating processes of virus, thus an attractive target for designing antiviral drugs. we screened several dna enzymes (dzs) containing the 10-23 catalytic motif against the influenza b virus bm2 rna. dzs directed against the predicted single-stran ...201323971908
[analysis of genetic variation of hemagglutinin and three internal genes of influenza b virus isolated in zhejiang province from 1999 to 2012].to explore the characteristics of the genetic variation of hemagglutinin( ha) and three internal genes coding for the nucleoprotein ( np) , matrix protein ( m) and nonstructural protein ( ns) of influenza b virus.201323958122
[molecular evolution of two lineages related to influenza b virus based on ha1 gene].to study the evolutionary characteristics and rules of two lineages on influenza b virus.201323937842
genetic diversity of influenza b virus in 2009-2010 and 2010-2011 in tunisia.the authors had for aim to characterize influenza b strains having circulated in tunisia to identify new mutations and compare them with reference strains.201323876206
[changes and analysis of peripheral white blood cells and lymphocyte subsets for patients with influenza b virus infection].to investigate the characters and changes of peripheral white blood cells and lymphocyte subsets of patients with influenza b virus infection and to provide evidences for diagnosis, treatment and prognosis of influenza b virus infection.201323855125
anti-influenza virus activity of two extracts of the blackcurrant (ribes nigrum l.) from new zealand and poland.we investigated the inhibitory effect of extracts of blackcurrant (ribes nigrum l.) from new zealand and poland on 4 strains of influenza virus (ifv) by the inhibition of virus adsorption; pandemic flu from 2009-2010 (ifv-ah1pdm), hong kong flu (ifv-ah3), oseltamivir phosphate-resistant russian flu (ifv-ah1tamr) and influenza virus type b (ifv-b). the inhibitory effect of the extracts of blackcurrant or blueberry on the infectivity of the virion were evaluated by the inhibition of virus adsorpti ...201323842512
recurring influenza b virus infections in seals. 201323750359
immunogenicity and safety of quadrivalent versus trivalent inactivated influenza vaccine: a randomized, controlled trial in adults.two phylogenetic lineages of influenza b virus coexist and circulate in the human population (b/yamagata and b/victoria) but only one b-strain is included in each seasonal vaccine. mismatch regularly occurs between the recommended and circulating b-strain. inclusion of both lineages in vaccines may offer better protection against influenza.201323688546
[virological characterization of influenza b virus in mainland china during 2011-2012].in order to understand the prevalence and variation of influenza b viruses, the antigenic and genetic characteristics of influenza b viruses circulating in mainland china during april, 2011 to march, 2012 were analyzed. the results showed the b victoria lineage viruses were much more prevalent than b yamagata lineage during this period, phylogenetic analysis showed vast majority of victoria lineage viruses belong to genetic group 1, intra-clade reassortant between ha1 and na gene was identified ...201323547377
mutations at the monomer-monomer interface away from the active site of influenza b virus neuraminidase reduces susceptibility to neuraminidase inhibitor drugs.amino acid changes in or near the active site of neuraminidase (na) in influenza viruses reduce the susceptibility to na inhibitor drugs. here, we report the recovery of three influenza b viruses with reduced susceptibilities to na inhibitors from human patients with no history of antiviral drug treatment. the three viruses were isolated by inoculating madin-darby canine kidney (mdck) cells with respiratory specimens from the patients. na inhibition assays demonstrated that two of the three isol ...201323529501
human monoclonal antibodies broadly neutralizing against influenza b virus.influenza virus has the ability to evade host immune surveillance through rapid viral genetic drift and reassortment; therefore, it remains a continuous public health threat. the development of vaccines producing broadly reactive antibodies, as well as therapeutic strategies using human neutralizing monoclonal antibodies (humabs) with global reactivity, has been gathering great interest recently. here, three hybridoma clones producing humabs against influenza b virus, designated 5a7, 3a2 and 10c ...201323408886
genetic analysis of influenza b viruses isolated in uganda during the 2009-2010 seasons.influenza b viruses can cause morbidity and mortality in humans but due to the lack of an animal reservoir are not associated with pandemics. because of this, there is relatively limited genetic sequences available for influenza b viruses, especially from developing countries. complete genome analysis of one influenza b virus and several gene segments of other influenza b viruses isolated from uganda from may 2009 through december 2010 was therefore undertaken in this study.201323289789
a cluster of patients infected with i221v influenza b virus variants with reduced oseltamivir susceptibility--north carolina and south carolina, 2010-2011.during 2010-2011, influenza b viruses with a novel neuraminidase substitution, denoted i221v (b/i221v), associated with reduced in vitro oseltamivir susceptibility were detected in north carolina.201323242536
[genetic variation of the hemagglutinin and neuraminidase of influenza b viruses isolated in ningbo during 2010-2012].to investigate the epidemiological characteristics of influenza b viruses and explore the genetic evolution characteristics of the hemagglutinin(ha) and neuraminidase(na) genes of local isolated strains in ningbo, southeast china, during 2010 to 2012.201324529267
vaccine for prevention of mild and moderate-to-severe influenza in children.commonly used trivalent vaccines contain one influenza b virus lineage and may be ineffective against viruses of the other b lineage. we evaluated the efficacy of a candidate inactivated quadrivalent influenza vaccine (qiv) containing both b lineages.201324328444
review of the clinical effectiveness of the neuraminidase inhibitors against influenza b viruses.influenza a and b viruses cause significant morbidity and mortality worldwide each year. the neuraminidase inhibitors (nais) are the most commonly used class of influenza antiviral drugs for the treatment of infected patients. in vitro studies have shown that influenza b viruses are significantly less susceptible to oseltamivir and other neuraminidase inhibitors compared with influenza a viruses. following analysis of published clinical studies, we show that oseltamivir does appear to have lower ...201324093683
influenza b virus has global ordered rna structure in (+) and (-) strands but relatively less stable predicted rna folding free energy than allowed by the encoded protein sequence.influenza a virus contributes to seasonal epidemics and pandemics and contains global ordered rna structure (gors) in the nucleoprotein (np), non-structural (ns), pb2, and m segments. a related virus, influenza b, is also a major annual public health threat, but unlike influenza a is very selective to human hosts. this study extends the search for gors to influenza b.201323958134
cross-reactive human b cell and t cell epitopes between influenza a and b viruses.influenza a and b viruses form different genera, which were originally distinguished by antigenic differences in their nucleoproteins and matrix 1 proteins. cross-protection between these two genera has not been observed in animal experiments, which is consistent with the low homology in viral proteins common to both viruses except for one of three polymerase proteins, polymerase basic 1 (pb1). recently, however, antibody and cd4+ t cell epitopes conserved between the two genera were identified ...201323886073
nuclear import of influenza b virus nucleoprotein: involvement of an n-terminal nuclear localization signal and a cleavage-protection motif.the nucleoprotein of influenza b virus (bnp) shares several characteristics with its influenza a virus counterpart (anp), including localization in the host's nucleus. however, while the nuclear localization signal(s) (nls) of anp are well characterized, little is known about those of bnp. in this study, we showed that the fusion protein bearing the bnp n-terminus fused with gfp (n70-gfp) is exclusively nuclear, and identified a highly conserved krxr motif spanning residues 44-47 as a putative n ...201323689061
detection of influenza a and b viruses with the sofia analyzer: a novel, rapid immunofluorescence-based in vitro diagnostic device.this report describes the clinical evaluation of a novel fluorescent immunoassay (fia), sofia influenza a+b fia (quidel, san diego, ca), for the rapid detection and differentiation of influenza a and b viruses. a total of 2,047 subjects provided nasal swabs and nasopharyngeal swabs or aspirates. the overall sensitivity and specificity for influenza a virus vs virus culture were 94% and 95%, respectively, and for influenza b virus were 89% and 96%, respectively. fourteen hundred and sixty-one spe ...201323596120
case-control study of the effectiveness of the 2010-2011 seasonal influenza vaccine for prevention of laboratory-confirmed influenza virus infection in the korean adult population.we evaluated the effectiveness of the 2010-2011 seasonal influenza vaccine for preventing laboratory-confirmed influenza in a south korean population. a retrospective case-control study was conducted among patients who visited selected hospitals from september 2010 to may 2011. a total of 483 laboratory-confirmed influenza patients were included in the analysis as case subjects. for each case patient, two types of control patients were chosen at a ratio of 1:1:1, and 966 control subjects were se ...201323576674
comparison of conventional lateral-flow assays and a new fluorescent immunoassay to detect influenza viruses.sofia, a novel, fluorescent lateral-flow immunoassay was compared with two conventional colorimetric assays, quickvue influenza a+b and directigen flu a+b, to identify influenza viral antigen from patient nasopharyngeal specimens. a total of 118 frozen original influenza-positive specimens and 57 prospective specimens were examined. using rt-pcr as a referee assay, sensitivity values (%) for influenza a/b of 80.0/74.8, 73.3/59.3 and 73.3/40.7 were obtained using the sofia, quickvue and directige ...201323458693
genomic adaptation of the isa virus to salmo salar codon usage.the isa virus (isav) is an orthomyxovirus whose genome encodes for at least 10 proteins. low protein identity and lack of genetic tools have hampered the study of the molecular mechanism behind its virulence. it has been shown that viral codon usage controls several processes such as translational efficiency, folding, tuning of protein expression, antigenicity and virulence. despite this, the possible role that adaptation to host codon usage plays in virulence and viral evolution has not been st ...201323829271
comparative evaluation of the nanosphere verigene rv+ assay and the simplexa flu a/b & rsv kit for detection of influenza and respiratory syncytial viruses.using retrospective (n = 200) and prospective (n = 150) nasopharyngeal specimens, we evaluated the nanosphere verigene rv+ and the focus diagnostics simplexa flu a/b & rsv tests. overall, rv+ demonstrated sensitivities and specificities of 96.6% and 100% for influenza a virus, 100% and 99.7% for influenza b virus, and 100% and 100% for respiratory syncytial virus (rsv), while the simplexa test sensitivities and specificities were 82.8 and 99.7%, 76.2 and 100%, and 94.6 and 100%, respectively.201323152547
emergency department surveillance as a proxy for the prediction of circulating respiratory viral disease in eastern ontario.seasonal outbreaks of winter respiratory viruses are responsible for increases in morbidity and mortality in the community. previous studies have used hospitalizations, intensive care unit and emergency department (ed) visits as indicators of seasonal influenza incidence.201324421826
a 4 year prospective study to determine risk factors for severe community acquired pneumonia in children in southern china.pneumonia is the major cause of death under 5 years. with high cap numbers in china and growing access to picus, factors associated with severe cap need to be determined to optimize care.201322778084
comparison of the simplexa flua/b & rsv direct assay and laboratory-developed real-time pcr assays for detection of respiratory virus.the results of the focus simplexa flua/b & rsv direct assay were compared to those of laboratory-developed reverse transcription pcr tests for 498 nasopharyngeal swabs. concordance rates were 96.6% (476/493; κ = 0.91), 97.6% (481/493; κ = 0.47), and 99.2% (488/492; κ = 0.94) for influenza a, influenza b, and respiratory syncytial virus, respectively.201324048529
evaluation of the simplexa flu a/b and rsv test for the rapid detection of influenza viruses.recently, various molecular systems have been introduced for the detection of influenza viruses. among these, the simplexa flu a/b and respiratory syncytial virus (rsv) test can provide results in approximately 2 hr. nasopharyngeal swabs from 241 patients (influenza a, n = 81; influenza b, n = 80; influenza a and influenza b mixed, n = 1; influenza a and rsv a mixed, n = 2; and influenza and rsv negative, n = 77) were analyzed using the simplexa flu a/b and rsv test, a conventional reverse-trans ...201323959434
sentinel surveillance of influenza and other respiratory viruses, brazil, 2000-2010.there are scanty data on the epidemiology of influenza and other respiratory viruses in south america and brazil. the aim of this study was to summarize the data from the brazilian surveillance system of influenza and other respiratory viruses and discuss the patterns of viral circulation. the system is based on detecting cases of influenza-like illness in sentinel sites and weekly collection of five nasopharyngeal secretions samples, which are processed in state public health laboratories for r ...201323287541
viral etiology of acute respiratory infections in hospitalized and outpatient children in buenos aires, argentina.to determine and compare the viral frequency, seasonality and clinical-demographic features in 2 groups of children (hospitalized versus outpatients) with acute respiratory infections.201323190781
update: influenza activity--united states and worldwide, may 19-september 28, 2013.during may 19-september 28, 2013,* the united states experienced low levels of seasonal influenza activity overall. influenza a (h1n1) pdm09 (ph1n1), influenza a (h3n2), and influenza b viruses were detected worldwide and were identified sporadically in the united states. in june, influenza a (h3n2) variant(†) viruses (h3n2)v were first detected in indiana, and between june 18 and september 28, a total of 20 cases of influenza a variant viruses ([h3n2]v and influenza a (h1n1) variant [h1n1]v) we ...201324153315
incidence of symptomatic a(h1n1)pdm09 influenza during the pandemic and post-pandemic periods in a rural indian community.data on influenza illness rates with population denominators are needed to quantify overall morbidity and to prioritize public health intervention strategies.201324103331
influenza transmission in a cohort of households with children: 2010-2011.households play a major role in community spread of influenza and are potential targets for mitigation strategies.201324086511
immunologic consequences of chemotherapy for ovarian cancer: impaired responses to the influenza vaccine.to examine the effect of chemotherapy for ovarian cancer on immunologic function and to define the effect on the serologic response to the influenza vaccine.201324036157
immunogenicity, reactogenicity and safety of an inactivated quadrivalent influenza vaccine candidate versus inactivated trivalent influenza vaccine: a phase iii, randomized trial in adults aged ≥18 years.two antigenically distinct influenza b lineages have co-circulated since the 1980s, yet inactivated trivalent influenza vaccines (tivs) include strains of influenza a/h1n1, a/h3n2, and only one influenza b from either the victoria or yamagata lineage. this means that exposure to b-lineage viruses mismatched to the tiv is frequent, reducing vaccine protection. formulations including both influenza b lineages could improve protection against circulating influenza b viruses. we assessed a candidate ...201323883186
long-term immunogenicity of the influenza vaccine at reduced intradermal and full intramuscular doses among healthy young adults.to prepare for vaccine shortages under an influenza pandemic, several antigen-sparing strategies have been investigated. this study was aimed to evaluate the immunogenicity of influenza vaccine at reduced intradermal and full intramuscular dose.201323858402
long-term and cross-reactive immunogenicity of inactivated trivalent influenza vaccine in the elderly: mf59-adjuvanted vaccine versus unadjuvanted vaccine.elderly people are at great risk for influenza-related serious complications. however, influenza vaccine-induced antibodies are believed to decline more rapidly in the elderly. this study was designed to evaluate the long-term and cross-reactive immunogenicity among those aged ≥65 years for two seasonal trivalent influenza vaccines during the 2009-2010 influenza season. one vaccine had the mf59 adjuvant, while the other did not contain an adjuvant. serum hemagglutinin inhibition (hi) titers were ...201323852684
immunogenicity and safety of an inactivated quadrivalent influenza vaccine candidate: a phase iii randomized controlled trial in children.mismatch between circulating influenza b viruses (yamagata and victoria lineages) and vaccine strains occurs frequently.201323847058
the safety and immunogenicity of trivalent inactivated influenza vaccination: a study of maternal-cord blood pairs in taiwan.there are little data about adverse effects and immunogenicity of flu vaccine in asian pregnant women.201323762229
influenza activity--united states, 2012-13 season and composition of the 2013-14 influenza vaccine.during the 2012-13 influenza season in the united states, influenza activity* increased through november and december before peaking in late december. influenza a (h3n2) viruses predominated overall, but influenza b viruses and, to a lesser extent, influenza a (h1n1)pdm09 (ph1n1) viruses also were reported in the united states. this influenza season was moderately severe, with a higher percentage of outpatient visits for influenza-like illness (ili), higher rates of hospitalization, and more rep ...201323760189
burden of seasonal and pandemic influenza-associated hospitalization during and after 2009 a(h1n1)pdm09 pandemic in a rural community in india.influenza is vaccine-preventable; however, the burden of severe influenza in india remains unknown. we conducted a population-based study to estimate the incidence of laboratory confirmed influenza-associated hospitalizations in a rural community in western india.201323690913
regional variation in mortality impact of the 2009 a(h1n1) influenza pandemic in china.laboratory-confirmed deaths grossly underestimate influenza mortality burden, so that reliable burden estimates are derived from indirect statistical studies, which are scarce in low- and middle-income settings.201323668477
evaluation of a polymerase chain reaction-electrospray ionization time-of-flight mass spectrometry for the detection and subtyping of influenza viruses in respiratory specimens.pcr coupled to electrospray ionization mass spectrometry technology (pcr/esi-tof-ms) (plex-id system, abbott ibis biosciences) was developed to characterize microbial pathogens.201323557709
a subregional analysis of epidemiologic and genetic characteristics of influenza a(h1n1)pdm09 in africa: senegal, cape verde, mauritania, and guinea, 2009-2010.during the pandemic 2009 episode, we conducted laboratory-based surveillance in four countries from west africa: senegal, mauritania, cape verde, and guinea. specimens were obtained from 3,155 patients: 2,264 patients from senegal, 498 patients from cape verde, 227 patients from mauritania, and 166 patients from guinea; 911 (28.9%) patients were positive for influenza, 826 (90.7%) patients were positive for influenza a, and 85 (9.3%) patients were positive for influenza b. among the influenza a ...201323509122
a randomized trial of candidate inactivated quadrivalent influenza vaccine versus trivalent influenza vaccines in children aged 3-17 years.two antigenically distinct influenza b lineages have cocirculated since 2001, yet trivalent influenza vaccines (tivs) contain 1 influenza b antigen, meaning lineage mismatch with the vaccine is frequent. we assessed a candidate inactivated quadrivalent influenza vaccine (qiv) containing both b lineages vs tiv in healthy children aged 3-17 years.201323470848
randomized controlled trial of high-dose intradermal versus standard-dose intramuscular influenza vaccine in organ transplant recipients.the immunogenicity of standard intramuscular (im) influenza vaccine is suboptimal in transplant recipients. also, recent studies suggest that alloantibody may be upregulated due to vaccination. we evaluated a novel high-dose intradermal (id) vaccine strategy. in conjunction, we assessed alloimmunity. transplant recipients were randomized to receive im or high-dose id vaccine. strain-specific serology and hla alloantibody production was determined pre- and postimmunization. in 212 evaluable patie ...201323406320
global assessment of resistance to neuraminidase inhibitors, 2008-2011: the influenza resistance information study (iris).following emergence of naturally occurring oseltamivir-resistant influenza a(h1n1) viruses, a global observational investigation, the influenza resistance information study (iris; nct00884117), was initiated in 2008 to study neuraminidase inhibitor (nai) resistance and clinical outcome.201323307766
prevention and control of influenza and dengue through vaccine development.influenza and dengue are viral illnesses of global public health importance, especially among children. accordingly, these diseases have been the focus of efforts to improve their prevention and control. influenza vaccination offers the best protection against clinical disease caused by strains contained within the specific year's formulation. it is not uncommon for there to be a mismatch between vaccine strains and circulating strains, particularly with regards to the b lineages. for more than ...201323910031
improved immunogenicity with high-dose seasonal influenza vaccine in hiv-infected persons: a single-center, parallel, randomized trial.hiv-infected persons have less robust antibody responses to influenza vaccines.201323277897
influenza antiviral resistance in the asia-pacific region during 2011.despite greater than 99% of influenza a viruses circulating in the asia-pacific region being resistant to the adamantane antiviral drugs in 2011, the large majority of influenza a (>97%) and b strains (∼99%) remained susceptible to the neuraminidase inhibitors oseltamivir and zanamivir. however, compared to the first year of the 2009 pandemic, cases of oseltamivir-resistant a(h1n1)pdm09 viruses with the h275y neuraminidase mutation increased in 2011, primarily due to an outbreak of oseltamivir-r ...201323274624
pandemic h1n1 influenza surveillance in haiti, july-december 2009.from june 2009 through december 2009, haiti conducted sentinel surveillance for influenza. 499 samples were collected and tested using real-time rt-pcr. 197 (39.5%) were positive for influenza, including 95 (48%) pandemic (h1n1) 2009, 57 (29%) seasonal influenza a and 45 (23%) influenza b. the median age of pandemic (h1n1) 2009 cases was 21.7; two-thirds of pandemic (h1n1) 2009 cases were in patients aged 6 years - 35 years. pandemic activity peaked in september and co-circulated with other infl ...201323199103
trends of influenza infection in suriname.the trends of influenza infection in suriname were assessed from february 2010 through february 2011. testing of 393 patients with symptoms of acute respiratory infection (ari) revealed 15.3% influenza b and 18.6% could be identified as influenza a positive, consisting of 56% influenza a(h1n1)pdm09 and 44% seasonal a(h3n2). influenza infection occurred throughout the year, and all three influenza types affected young children as the primary population. the annual incidence of a(h1n1)pdm09 was 6. ...201323136977
a clinical trial of intravenous peramivir compared with oral oseltamivir for the treatment of seasonal influenza in hospitalized adults.seasonal interpandemic influenza causes >200,000 annual hospitalizations in the united states. optimal antiviral treatment in hospitalized patients is not established.201323111657
sentinel surveillance for influenza and other respiratory viruses in côte d'ivoire, 2003-2010.many countries in africa have lacked sentinel surveillance systems for influenza and are under-represented in data used for global vaccine strain selection.201322863403
age-specific vaccine effectiveness of seasonal 2010/2011 and pandemic influenza a(h1n1) 2009 vaccines in preventing influenza in the united kingdom.an analysis was undertaken to measure age-specific vaccine effectiveness (ve) of 2010/11 trivalent seasonal influenza vaccine (tiv) and monovalent 2009 pandemic influenza vaccine (piv) administered in 2009/2010. the test-negative case-control study design was employed based on patients consulting primary care. overall tiv effectiveness, adjusted for age and month, against confirmed influenza a(h1n1)pdm 2009 infection was 56% (95% ci 42-66); age-specific adjusted ve was 87% (95% ci 45-97) in <5-y ...201322691710
burden and characteristics of influenza a and b in danish intensive care units during the 2009/10 and 2010/11 influenza seasons.influenza surveillance in danish intensive care units (icus) was performed during the 2009/10 and 2010/11 influenza seasons to monitor the burden on icus. all 44 danish icus reported aggregate data for incidence and point prevalence, and case-based demographical and clinical parameters. additional data on microbiological testing, vaccination and death were obtained from national registers. ninety-six patients with influenza a(h1n1)pdm09 were recorded in 2009/10; 106 with influenza a and 42 with ...201322793496
a prospective intervention study on higher-dose oseltamivir treatment in adults hospitalized with influenza a and b infections.it is unclear if higher-dose oseltamivir provides benefit beyond the standard dose in influenza patients who require hospitalization.201324046309
early estimates of seasonal influenza vaccine effectiveness in europe: results from the i-move multicentre case-control study, 2012/13.we conducted a test-negative case-control study based in five european sentinel surveillance networks. the early 2012/13 adjusted influenza vaccine effectiveness was 78.2% (95% ci: 18.0 to 94.2) against influenza b, 62.1% (95% ci: -22.9 to 88.3%) against a(h1) pdm09, 41.9 (95% ci: -67.1 to 79.8) against a(h3n2) and 50.4% (95% ci: -20.7 to 79.6) against all influenza types in the target groups for vaccination. efforts to improve influenza vaccines should continue to better protect those at risk o ...201323449183
update: influenza activity - united states, september 30, 2012-february 9, 2013.influenza activity in the united states began to increase in mid-november and remained elevated through february 9, 2013. during that time, influenza a (h3n2) viruses predominated overall, followed by influenza b viruses. this report summarizes u.s. influenza activity* since the beginning of the 2012-13 influenza season and updates the previous summary.201323425961
vaccine effectiveness of 2011/12 trivalent seasonal influenza vaccine in preventing laboratory-confirmed influenza in primary care in the united kingdom: evidence of waning intra-seasonal protection.the 2011/12 season was characterised by unusually late influenza a (h3n2) activity in the united kingdom (uk). we measured vaccine effectiveness (ve) of the 2011/12 trivalent seasonal influenza vaccine (tiv) in a test-negative case–control study in primary care. overall ve against confirmed influenza a (h3n2) infection, adjusted for age, surveillance scheme and month, was 23% (95% confidence interval (ci): -10 to 47). stratified analysis by time period gave an adjusted ve of 43% (95% ci: -34 to ...201323399424
recrudescent wave of a/h1n1pdm09 influenza viruses in winter 2012-2013 in kashmir, india.some parts of world, including india observed a recrudescent wave of influenza a/h1n1pdm09 in 2012. we undertook a study to examine the circulating influenza strains, their clinical association and antigenic characteristics to understand the recrudescent wave of a/h1n1pdm09 from november 26, 2012 to feb 28, 2013 in kashmir, india. of the 751 patients (545 outpatient and 206 hospitalized) presenting with acute respiratory infection at a tertiary care hospital in srinagar; 184 (24.5%) tested posit ...201324818063
c-3 halo and 3-methyl substituted 5'-nor-3-deazaaristeromycins: synthesis and antiviral properties.to expand on the antiviral properties of 5'-noraristeromycin, synthetic entry into 3-substituted 3-deaza-5'-noraristeromyin derivatives (i.e., bromo, 4; iodo, 5; chloro, 6; and, methyl, 7) has been accomplished from a common intermediate. an extensive antiviral analysis showed 7 to be basically inactive (except for weak effects against vsv) and there were no general trends among the halo compounds (except versus reovirus-1 and influenza b). individually, compound 4 was most favorable towards hcm ...201323176752
an outbreak of influenza b in an isolated nomadic community in jammu & kashmir, india.community outbreaks of disease amongst nomadic populations generally remain undocumented. following a reported increase in acute respiratory tract infections (ari) in may 2011 in a nomadic population of sangerwini in jammu & kashmir, india, we examined the patients with ari symptoms and their nasal swabs were tested for influenza virus.201324521649
characterization of regional influenza seasonality patterns in china and implications for vaccination strategies: spatio-temporal modeling of surveillance data.the complexity of influenza seasonal patterns in the inter-tropical zone impedes the establishment of effective routine immunization programs. china is a climatologically and economically diverse country, which has yet to establish a national influenza vaccination program. here we characterize the diversity of influenza seasonality in china and make recommendations to guide future vaccination programs.201324348203
interferon-induced isg15 pathway: an ongoing virus-host battle.isg15 is an interferon (ifn)-induced ubiquitin-like protein that is conjugated to target proteins via the sequential action of three enzymes that are also induced by ifn. unlike ubiquitin, which is highly conserved, the sequence of isg15 varies between species. isg15 conjugation inhibits many viruses, and free (unconjugated) isg15 can also act as an antiviral protein. in this review, we focus on the antiviral role of isg15 conjugation and on countermeasures employed by several viruses. the count ...201323414970
inflammatory response of mast cells during influenza a virus infection is mediated by active infection and rig-i signaling.influenza a virus (iav) is a major respiratory pathogen of both humans and animals. the lung is protected from pathogens by alveolar epithelial cells, tissue-resident alveolar macrophages, dendritic cells, and mast cells. the role of alveolar epithelial cells, endothelial cells, and alveolar macrophages during iav infection has been studied previously. in this study, we address the role of mast cells during iav infection. respiratory infection with a/wsn/33 causes significant disease and immunop ...201323526820
evidence for a crucial role of a host non-coding rna in influenza a virus replication.a growing body of evidence suggests the non-protein coding human genome is of vital importance for human cell function. besides small rnas, the diverse class of long non-coding rnas (lncrnas) recently came into focus. however, their relevance for infection, a major evolutionary driving force, remains elusive. using two commercially available microarray systems, namely ncode™ and sureprint™ g3, we identified differential expression of 42 ncrnas during influenza a virus (iav) infection in human lu ...201324440876
comparison of the filmarray rp, verigene rv+, and prodesse proflu+/fast+ multiplex platforms for detection of influenza viruses in clinical samples from the 2011-2012 influenza season in belgium.respiratory tract infections (rtis) are caused by a plethora of viral and bacterial pathogens. in particular, lower rtis are a leading cause of hospitalization and mortality. timely detection of the infecting respiratory pathogens is crucial to optimize treatment and care. in this study, three u.s. food and drug administration-approved molecular multiplex platforms (prodesse proflu+/fast+, filmarray rp, and verigene rv+) were evaluated for influenza virus detection in 171 clinical samples collec ...201323824777
comparison of the biofire filmarray rp, genmark esensor rvp, luminex xtag rvpv1, and luminex xtag rvp fast multiplex assays for detection of respiratory viruses.there are several u.s. fda-cleared molecular respiratory virus panels available today, each with advantages and disadvantages. this study compares four multiplex panels, the biofire diagnostics filmarray rp (respiratory panel), the genmark dx esensor rvp (respiratory viral panel), the luminex xtag rvpv1, and the luminex xtag rvp fast. three hundred specimens (200 retrospective and 100 consecutive) were tested using all four platforms to determine performance characteristics. the overall sensitiv ...201323486707
duplex molecular assay intended for point-of-care diagnosis of influenza a/b virus infection.early diagnosis and management of influenza virus infection directly correlates with the effectiveness in disease control. current molecular influenza virus tests were designed for use in diagnostic testing facilities, where sophisticated equipment and highly trained technicians are available. a longer turnaround time for the centralized testing than when testing near the sample source could delay the initiation of medical intervention, thereby reducing the efficacy of antiviral treatment. the n ...201323850955
evaluation of the filmarray® respiratory panel for clinical use in a large children's hospital.respiratory pathogens are a leading cause of hospital admission and traditional detection methods are time consuming and insensitive. multiplex molecular detection methods have recently been investigated in hope of replacing these traditional techniques with rapid panel-based testing.201323424157
antiviral activity of isatis indigotica root-derived clemastanin b against human and avian influenza a and b viruses in vitro.clemastanin b, 7s,8r,8'r-(-)-lariciresinol-4,4'-bis-o-β-d-glucopyranoside, is one of the major lignans extracted from isatis indigotica root (iir). in this study, the anti-influenza activities of clemastanin b were evaluated in vitro. clemastanin b was found to inhibit different subtypes of human (h1n1, including swine-origin h1n1; h3n2 and influenza b) and avian influenza viruses (h6n2, h7n3, h9n2) at different magnitudes of activity (ic50 0.087-0.72 mg/ml) while this compound was inactive agai ...201323403777
synthesis and anti-influenza virus activities of a novel class of gastrodin derivatives.a series of substituted aryl glycoside analogues of gastrodin have been identified as potential anti-influenza agents. the most potent inhibitor 1a exhibited moderate inhibitory activity against the a/hanfang/359/95(h3n2) and a/fm/1/47(h1n1) strains of the influenza a virus (ic(50) values of 44.40 and 34.45 μm, respectively) and the oseltamivir-null b/jifang/13/97 strain of influenza b (ic(50) value of 33.01 μm). in this article, multiple doses of compound 1a (80 mg/kg/day, oral administration) ...201323531598
viral causes of acute respiratory infection among egyptian children hospitalized with severe acute asthma exacerbation.viral respiratory infections are associated with nearly 80% of asthma exacerbation episodes. these can have severe adverse outcomes in patients with established asthma.201323528533
application of three duplex real-time pcr assays for simultaneous detection of human seasonal and avian influenza viruses.this study was performed to develop real-time pcr (qpcr) for detection of human seasonal and avian influenza viruses in duplex format. first duplex qpcr detects haemagglutinin (ha) gene of influenza virus a(h1n1)pdm09 and ha gene of influenza virus a(h3n2), the second reaction detects neuraminidase (na) gene of influenza virus a(h3n2) and na gene of influenza virus a(h1n1)pdm09 and a(h5n1), and the third reaction detects ha gene of influenza a(h5n1) and nonstructural protein gene of influenza b ...201323515874
impact of influenza a(h1n1)pdm09 virus on circulation dynamics of seasonal influenza strains in kenya.we describe virus variations from patients with influenza-like illness before and after the appearance of influenza a(h1n1)pdm09 in kenya during january 2008-july 2011. a total of 11,592 nasopharyngeal swabs were collected from consenting patients. seasonal influenza b, a/h1n1, a/h3n2, a/h5n1, and influenza a(h1n1)pdm09 viruses were detected by real-time reverse transcription-polymerase chain reaction. of patients enrolled, 2073 (17.9%) had influenza. a total of 1,524 (73.4%) of 2,073 samples we ...201323458953
efficient synthesis of 3h,3'h-spiro[benzofuran-2,1'-isobenzofuran]-3,3'-dione as novel skeletons specifically for influenza virus type b inhibition.an efficient and novel two step synthetic procedure to prepare various substituted 3h,3'h-spiro[benzofuran-2,1'-isobenzofuran]-3,3'-diones a, was established from very simple and easily available starting materials. the developed method is a robust and general approach for the synthesis of these structures. the prepared compounds were tested against influenza virus type a viz., a/taiwan/1/86 (h1n1), a/hong kong/8/68 (h3n2) and type b viz., b/panama/45/90, b/taiwan/2/62, b/lee/40, b/brisbane/60/2 ...201323419738
first introduction of pandemic influenza a/h1n1 and detection of respiratory viruses in pediatric patients in central african republic.acute viral respiratory illnesses in children in sub-saharan africa have received relatively little attention, although they are much more frequent causes of morbidity and mortality than in developed countries. active surveillance is essential to identify the causative agents and to improve clinical management, especially in the context of possible circulation of pandemic viruses.201323391188
Refresh
firstprev
12345678910...
nextlast
Displaying items 801 - 900 of 4830