Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| detection of hepatitis e virus rna in raw sausages and liver sausages from retail in germany using an optimized method. | hepatitis e virus (hev) is a pathogen of increasing importance, which can be zoonotically transmitted from domestic pigs, wild boar, and deer to humans. foodborne transmission by consumption of raw and undercooked liver, meat, or sausages prepared from infected animals has been documented. the aim of this study was to investigate the distribution of hev in different types of sausages sold in germany. as no standardized methods for hev detection in food exist, several techniques of sample homogen ... | 2015 | 26433460 |
| serological and molecular investigation of swine hepatitis e virus in pigs raised in southern italy. | hepatitis e virus (hev) infection is a common acute hepatitis transmitted by the fecal-oral route. in developed countries, the virus has a zoonotic potential, and domestic pigs and wild boars are considered main reservoirs. to assess the prevalence of hev-positive animals in the calabria region (southern italy) on a serological and molecular level, a total of 216 autochthonous healthy pigs (apulo-calabrese breed) were sampled. both sera and feces were collected. pigs were grouped based on age: 1 ... | 2015 | 26555536 |
| epidemiology of leptospirosis in africa: a systematic review of a neglected zoonosis and a paradigm for 'one health' in africa. | leptospirosis is an important but neglected bacterial zoonosis that has been largely overlooked in africa. in this systematic review, we aimed to summarise and compare current knowledge of: (1) the geographic distribution, prevalence, incidence and diversity of acute human leptospirosis in africa; and (2) the geographic distribution, host range, prevalence and diversity of leptospira spp. infection in animal hosts in africa. | 2015 | 26368568 |
| combined inhibition of complement and cd14 improved outcome in porcine polymicrobial sepsis. | sepsis is an exaggerated and dysfunctional immune response to infection. activation of innate immunity recognition systems including complement and the toll-like receptor family initiate this disproportionate inflammatory response. the aim of this study was to explore the effect of combined inhibition of the complement component c5 and the toll-like receptor co-factor cd14 on survival, hemodynamic parameters and systemic inflammation including complement activation in a clinically relevant porci ... | 2015 | 26612199 |
| serosurvey of smooth brucella, leptospira spp. and toxoplasma gondii in free-ranging jaguars (panthera onca) and domestic animals from brazil. | this study investigated the exposure of jaguar populations and domestic animals to smooth brucella, leptospira spp. and toxoplasma gondii in the cerrado, pantanal and amazon biomes of brazil. between february 2000 and january 2010, serum samples from 31 jaguars (panthera onca), 1,245 cattle (bos taurus), 168 domestic dogs (canis lupus familiaris) and 29 domestic cats (felis catus) were collected and analysed by rose bengal test for smooth brucella, microscopic agglutination test for leptospira s ... | 2015 | 26605787 |
| a lentiviral vector expressing japanese encephalitis virus-like particles elicits broad neutralizing antibody response in pigs. | japanese encephalitis virus (jev) is the major cause of viral encephalitis in southeast asia. vaccination of domestic pigs has been suggested as a "one health" strategy to reduce viral disease transmission to humans. the efficiency of two lentiviral trip/jev vectors expressing the jev envelope prm and e glycoproteins at eliciting protective humoral response was assessed in a mouse model and piglets. | 2015 | 26437302 |
| redox state of flavin adenine dinucleotide drives substrate binding and product release in escherichia coli succinate dehydrogenase. | the complex ii family of enzymes, comprising respiratory succinate dehydrogenases and fumarate reductases, catalyzes reversible interconversion of succinate and fumarate. in contrast to the covalent flavin adenine dinucleotide (fad) cofactor assembled in these enzymes, soluble fumarate reductases (e.g., those from shewanella frigidimarina) that assemble a noncovalent fad cannot catalyze succinate oxidation but retain the ability to reduce fumarate. in this study, an sdha-h45a variant that elimin ... | 2015 | 25569225 |
| novel real-time pcr detection assay for brucella suis. | brucella suis is the causative agent of brucellosis in suidae and is differentiated into five biovars (bv). biovars 1 and 3 possess zoonotic potential and can infect humans, whereas biovar 2 represents the main source of brucellosis in feral and domestic pigs in europe. both aspects, the zoonotic threat and the economic loss, emphasize the necessity to monitor feral and domestic pig populations. available serological or pcr based methods lack sensitivity and specificity. | 2015 | 26392898 |
| pbmc transcriptomic responses to primary and secondary vaccination differ due to divergent lean growth and antibody titers in a pig model. | the genetic relationship between immune responsiveness and performance is not well understood, but a major topic of the evolution of resource allocation and of relevance in human medicine and livestock breeding, for instance. this study aims to show differences of transcript abundance changes during the time intervals before and after two tetanus toxoid (tt) vaccinations in domestic pigs differing in lean growth (lg) and anti-tt-antibody titers (ab) parameters of performance and immunocompetence ... | 2015 | 26175500 |
| standardization of the experimental autoimmune myasthenia gravis (eamg) model by immunization of rats with torpedo californica acetylcholine receptors--recommendations for methods and experimental designs. | myasthenia gravis (mg) with antibodies against the acetylcholine receptor (achr) is characterized by a chronic, fatigable weakness of voluntary muscles. the production of autoantibodies involves the dysregulation of t cells which provide the environment for the development of autoreactive b cells. the symptoms are caused by destruction of the postsynaptic membrane and degradation of the achr by igg autoantibodies, predominantly of the g1 and g3 subclasses. active immunization of animals with ach ... | 2015 | 25796590 |
| experimental infection of ornithodoros erraticus sensu stricto with two portuguese african swine fever virus strains. study of factors involved in the dynamics of infection in ticks. | african swine fever (asf) is a frequently devastating hemorrhagic disease of domestic pigs and wild boar and ornithodoros erraticus sensu stricto argasid ticks are the only biological vectors of african swine fever virus (asfv) known to occur in europe. recently this disease emerged in eastern europe and russian federation, showing a huge potential for a rapid spread between countries. there is some risk of re-emergence of asf in the countries where these ticks exist, that can contribute for the ... | 2015 | 26366570 |
| bat and pig ifn-induced transmembrane protein 3 restrict cell entry by influenza virus and lyssaviruses. | ifn-induced transmembrane protein 3 (ifitm3) is a restriction factor that blocks cytosolic entry of numerous viruses that utilize acidic endosomal entry pathways. in humans and mice, ifitm3 limits influenza-induced morbidity and mortality. although many ifitm3-sensitive viruses are zoonotic, whether ifitms function as antiviral restriction factors in mammalian species other than humans and mice is unknown. here, ifitm3 orthologues in the microbat (myotis myotis) and pig (sus scrofa domesticus) w ... | 2015 | 25614588 |
| estimating the basic reproductive number (r0) for african swine fever virus (asfv) transmission between pig herds in uganda. | african swine fever (asf) is a highly contagious, lethal and economically devastating haemorrhagic disease of domestic pigs. insights into the dynamics and scale of virus transmission can be obtained from estimates of the basic reproduction number (r0). we estimate r0 for asf virus in small holder, free-range pig production system in gulu, uganda. the estimation was based on data collected from outbreaks that affected 43 villages (out of the 289 villages with an overall pig population of 26,570) ... | 2015 | 25938429 |
| anaplasma sp. and hemoplasma infection in leopard cats (prionailurus bengalensis euptilurus) from korea. | this study examined the occurrence of anaplasma spp. and hemoplasma infection in leopard cats, prionailurus bengalensis euptilurus, in korea. twenty-nine biological samples were tested by molecular analysis. two (6.9%) and eight (27.6%) tested specimens were positive for anaplasma bovis and hemoplasma infection, respectively. based on our results, anaplasma/ehrlichia spp. and hemoplasma are regularly infecting leopard cat populations of korea. considering their endangered status, regular monitor ... | 2015 | 26040618 |
| experimental infection with the toxoplasma gondii me-49 strain in the brazilian br-1 mini pig is a suitable animal model for human toxoplasmosis. | toxoplasma gondii causes toxoplasmosis, a worldwide disease. experimentation with pigs is necessary for the development of new therapeutic approaches to human diseases. br-1 mini pigs were intramuscularly infected with t. gondii with tachyzoites (rh strain) or orally infected with cysts (me-49 strain). haematology and serum biochemistry were analysed and buffy coat cells were inoculated in mice to determine tachyzoite circulation. no alterations were observed in erythrocyte and platelet values; ... | 2015 | 25742268 |
| effect of body mass and clothing on carrion entomofauna. | carcass mass largely affects pattern and rate of carrion decomposition. supposedly, it is similarly important for carrion entomofauna; however, most of its likely effects have not been tested experimentally. here, simultaneous effects of carcass mass and clothing are analyzed. a factorial block experiment with four levels of carcass mass (small carcasses 5-15 kg, medium carcasses 15.1-30 kg, medium/large carcasses 35-50 kg, large carcasses 55-70 kg) and two levels of carcass clothing (clothed an ... | 2015 | 25874664 |
| single nucleotide markers of d-loop for identification of indian wild pig (sus scrofa cristatus). | partial fragment of d-loop region extending from 35 to 770 were compared with corresponding sequences of 16 wild pigs and 9 domestic pig breeds from different parts of the world for detection of single nucleotide polymorphism (snp) markers in the region. the paper also reappraises snp markers from two fragments of cytochrome b gene and a fragment 12s rrna gene distinguishing the indian wild pig from other pig species of the world. | 2015 | 27047129 |
| exploring the genetic signature of body size in yucatan miniature pig. | since being domesticated about 10,000-12,000 years ago, domestic pigs (sus scrofa domesticus) have been selected for traits of economic importance, in particular large body size. however, yucatan miniature pigs have been selected for small body size to withstand high temperature environment and for laboratory use. this renders the yucatan miniature pig a valuable model for understanding the evolution of body size. we investigate the genetic signature for selection of body size in the yucatan min ... | 2015 | 25885114 |
| staphylococcus aureus induces hypoxia and cellular damage in porcine dermal explants. | we developed a porcine dermal explant model to determine the extent to which staphylococcus aureus biofilm communities deplete oxygen, change ph, and produce damage in underlying tissue. microelectrode measurements demonstrated that dissolved oxygen (do) in biofilm-free dermal tissue was 4.45 ± 1.17 mg/liter, while do levels for biofilm-infected tissue declined sharply from the surface, with no measurable oxygen detectable in the underlying dermal tissue. magnetic resonance imaging demonstrated ... | 2015 | 25847960 |
| serological prevalence of viral agents that induce reproductive failure in south korean wild boar. | viral agents associated with reproductive failure such as aujeszky's disease virus (adv), encephalomyocarditis virus (emcv), and porcine parvovirus (ppv) have also been identified in european wild boar. to screen for the presence of antibodies against adv, emcv, and ppv from wild boar (sus scrofa) in south korea, 481 serum samples were collected from wild boar hunted between december 2010 and may 2011. | 2015 | 25888836 |
| corrigendum: cellular scaling rules for the brain of artiodactyla include a highly folded cortex with few neurons. | [this corrects the article on p. 128 in vol. 8, pmid: 25429261.]. | 2015 | 25859187 |
| snp marker discovery in koala tlr genes. | toll-like receptors (tlrs) play a crucial role in the early defence against invading pathogens, yet our understanding of tlrs in marsupial immunity is limited. here, we describe the characterisation of nine tlrs from a koala immune tissue transcriptome and one tlr from a draft sequence of the koala genome and the subsequent development of an assay to study genetic diversity in these genes. we surveyed genetic diversity in 20 koalas from new south wales, australia and showed that one gene, tlr10 ... | 2015 | 25799012 |
| genome-wide association analyses reveal significant loci and strong candidate genes for growth and fatness traits in two pig populations. | recently, genome-wide association studies (gwas) have been reported on various pig traits. we performed a gwas to analyze 22 traits related to growth and fatness on two pig populations: a white duroc × erhualian f2 intercross population and a chinese sutai half-sib population. | 2015 | 25885760 |
| investigating the role of free-ranging wild boar (sus scrofa) in the re-emergence of enzootic pneumonia in domestic pig herds: a pathological, prevalence and risk-factor study. | enzootic pneumonia (ep) caused by mycoplasma hyopneumoniae has a significant economic impact on domestic pig production. a control program carried out from 1999 to 2003 successfully reduced disease occurrence in domestic pigs in switzerland, but recurrent outbreaks suggested a potential role of free-ranging wild boar (sus scrofa) as a source of re-infection. since little is known on the epidemiology of ep in wild boar populations, our aims were: (1) to estimate the prevalence of m. hyopneumoniae ... | 2015 | 25747151 |
| the relation between catheter occlusion and backflow during intraparenchymal cerebral infusions. | background/aims: the distribution of infusate into the brain by convection-enhanced delivery can be affected by backflow along the catheter shaft. this work assesses the following: (1) whether tissue coring and occlusion of the catheter lumen occurs when an open end-port catheter is inserted, (2) whether there is a relationship between intracatheter pressure and backflow, and (3) whether catheter occlusion increases backflow. methods: freshly excised monkey brains were used to assess tissue cori ... | 2015 | 25721097 |
| when pigs fly, ucp1 makes heat. | brown and beige adipose tissue may represent important therapeutic targets for the treatment of diabetes and obesity as these organs dissipate nutrient energy as heat through the thermogenic uncoupling protein 1 (ucp1). while mice are commonly used to mimic the potential effects of brown/beige adipose tissue that may act in human metabolism, new animal models are edging into the market for translational medicine. pigs reflect human metabolism better than mice in multiple parameters such as obesi ... | 2015 | 25973382 |
| a large animal model of spinal muscular atrophy and correction of phenotype. | spinal muscular atrophy (sma) is caused by reduced levels of survival motor neuron (smn) protein, which results in motoneuron loss. therapeutic strategies to increase smn levels including drug compounds, antisense oligonucleotides, and scaav9 gene therapy have proved effective in mice. we wished to determine whether reduction of smn in postnatal motoneurons resulted in sma in a large animal model, whether sma could be corrected after development of muscle weakness, and the response of clinically ... | 2015 | 25516063 |
| gene coexpression networks reveal key drivers of phenotypic divergence in porcine muscle. | domestication of the wild pig has led to obese and lean phenotype breeds, and evolutionary genome research has sought to identify the regulatory mechanisms underlying this phenotypic diversity. however, revealing the molecular mechanisms underlying muscle phenotype variation based on differentially expressed genes has proved to be difficult. to characterize the mechanisms regulating muscle phenotype variation under artificial selection, we aimed to provide an integrated view of genome organizati ... | 2015 | 25651817 |
| phenotype and animal domestication: a study of dental variation between domestic, wild, captive, hybrid and insular sus scrofa. | identifying the phenotypic responses to domestication remains a long-standing and important question for researchers studying its early history. the great diversity in domestic animals and plants that exists today bears testament to the profound changes that domestication has induced in their ancestral wild forms over the last millennia. domestication is a complex evolutionary process in which wild organisms are moved to new anthropogenic environments. although modern genetics are significantly ... | 2015 | 25648385 |
| metallothioneins, unconventional proteins from unconventional animals: a long journey from nematodes to mammals. | metallothioneins (mts) are ubiquitous low molecular weight cysteine-rich proteins characterized by high affinity for d10 electron configuration metals, including essential (zn and cu) and non-essential (cd and hg) trace elements. the biological role of these ancient and well-conserved multifunctional proteins has been debated since mts were first discovered in 1957. their main hypothesized functions are: (1) homeostasis of zn and cu; (2) detoxification of cd, and hg; and (3) free radical scaveng ... | 2014 | 24970224 |
| cone photoreceptors develop normally in the absence of functional rod photoreceptors in a transgenic swine model of retinitis pigmentosa. | human and swine retinas have morphological and functional similarities. in the absence of primate models, the swine is an attractive model to study retinal function and disease, with its cone-rich visual streak, our ability to manipulate their genome, and the differences in susceptibility of rod and cone photoreceptors to disease. we characterized the normal development of cone function and its subsequent decline in a p23h rhodopsin transgenic (tgp23h) miniswine model of autosomal dominant rp. | 2014 | 24618325 |
| whole-genome sequencing of berkshire (european native pig) provides insights into its origin and domestication. | domesticated organisms have experienced strong selective pressures directed at genes or genomic regions controlling traits of biological, agricultural or medical importance. the genome of native and domesticated pigs provide a unique opportunity for tracing the history of domestication and identifying signatures of artificial selection. here we used whole-genome sequencing to explore the genetic relationships among the european native pig berkshire and breeds that are distributed worldwide, and ... | 2014 | 24728479 |
| technical development of a new semispherical radiofrequency bipolar device (ronja): ex vivo and in vivo studies. | the aim of this study is to inform about the development of a new semispherical surgical instrument for the bipolar multielectrode radiofrequency liver ablation. present tools are universal; however they have several disadvantages such as ablation of healthy tissue, numerous needle punctures, and, therefore, longer operating procedure. our newly designed and tested semispherical surgical tool can solve some of these disadvantages. by conducting an in vivo study on a set of 12 pigs, randomly divi ... | 2014 | 24812620 |
| inflammation blood and tissue factors of plaque growth in an experimental model evidenced by a systems approach. | the multifactorial pathogenesis of coronary atherosclerotic lesion formation has been investigated in a swine model of high cholesterol diet induced atherogenesis and data processed by a systems approach. | 2014 | 24778640 |
| identification of g protein-coupled estrogen receptor in human and pig spermatozoa. | estrogens are known to influence functional properties of mammalian spermatozoa inducing rapid responses through the classical estrogen receptors (erα and erβ). recently, the g protein-coupled estrogen receptor (gper) has been identified as mediator of fast non-genomic estrogen effects in different cells. this work investigated the expression of gper in human and pig spermatozoa using immunofluorescence, western blot analysis and rt-pcr. gper was found to be confined to the mid-piece of human sp ... | 2014 | 24697543 |
| black cobra (naja naja karachiensis) lysates exhibit broad-spectrum antimicrobial activities. | it is hypothesized that animals living in polluted environments possess antimicrobials to counter pathogenic microbes. the fact that snakes feed on germ-infested rodents suggests that they encounter pathogenic microbes and likely possess antimicrobials. the venom is used only to paralyze the rodent, but the ability of snakes to counter potential infections in the gut due to disease-ridden rodents requires robust action of the immune system against a broad range of pathogens. to test this hypothe ... | 2014 | 24625321 |
| novel avian-origin influenza a (h7n9) virus attachment to the respiratory tract of five animal models. | we determined the pattern of attachment of the avian-origin h7n9 influenza viruses a/anhui/1/2013 and a/shanghai/1/2013 to the respiratory tract in ferrets, macaques, mice, pigs, and guinea pigs and compared it to that in humans. the h7n9 attachment pattern in macaques, mice, and to a lesser extent pigs and guinea pigs resembled that in humans more closely than the attachment pattern in ferrets. this information contributes to our knowledge of the different animal models for influenza. | 2014 | 24478425 |
| genome-wide association studies for hematological traits in chinese sutai pigs. | it has been shown that hematological traits are strongly associated with the metabolism and the immune system in domestic pig. however, little is known about the genetic architecture of hematological traits. to identify quantitative trait loci (qtl) controlling hematological traits, we performed single marker genome-wide association studies (gwas) and haplotype analysis for 15 hematological traits in 495 chinese sutai pigs. | 2014 | 24674592 |
| medieval horse stable; the results of multi proxy interdisciplinary research. | a multi proxy approach was applied in the reconstruction of the architecture of medieval horse stable architecture, the maintenance practices associated with that structure as well as horse alimentation at the beginning of 13th century in central europe. finally, an interpretation of the local vegetation structure along morava river, czech republic is presented. the investigated stable experienced two construction phases. the infill was well preserved and its composition reflects maintenance pra ... | 2014 | 24670874 |
| animal study assessing safety of an acoustic coupling fluid that holds the potential to avoid surgically induced artifacts in 3d ultrasound guided operations. | use of ultrasound in brain tumor surgery is common. the difference in attenuation between brain and isotonic saline may cause artifacts that degrade the ultrasound images, potentially affecting resection grades and safety. our research group has developed an acoustic coupling fluid that attenuates ultrasound energy like the normal brain. we aimed to test in animals if the newly developed acoustic coupling fluid may have harmful effects. | 2014 | 24666721 |
| xenoantibody response to porcine islet cell transplantation using gtko, cd55, cd59, and fucosyltransferase multiple transgenic donors. | promising developments in porcine islet xenotransplantation could resolve the donor pancreas shortage for patients with type 1 diabetes. using α1,3-galactosyltransferase gene knockout (gtko) donor pigs with multiple transgenes should extend xenoislet survival via reducing complement activation, thrombus formation, and the requirement for exogenous immune suppression. studying the xenoantibody response to gtko/hcd55/hcd59/hht islets in the pig-to-baboon model, and comparing it with previously ana ... | 2014 | 24645827 |
| lapita diet in remote oceania: new stable isotope evidence from the 3000-year-old teouma site, efate island, vanuatu. | remote oceania was colonized ca. 3000 bp by populations associated with the lapita cultural complex, marking a major event in the prehistoric settlement of the pacific islands. although over 250 lapita sites have been found throughout the western pacific, human remains associated with lapita period sites are rare. the site of teouma, on efate island, vanuatu has yielded the largest burial assemblage (n=68 inhumations) of lapita period humans ever discovered, providing a unique opportunity for as ... | 2014 | 24598939 |
| assessment of regional ventilation distribution: comparison of vibration response imaging (vri) with electrical impedance tomography (eit). | vibration response imaging (vri) is a bedside technology to monitor ventilation by detecting lung sound vibrations. it is currently unknown whether vri is able to accurately monitor the local distribution of ventilation within the lungs. we therefore compared vri to electrical impedance tomography (eit), an established technique used for the assessment of regional ventilation. | 2014 | 24475160 |
| the role of viral and host micrornas in the aujeszky's disease virus during the infection process. | porcine production is a primary market in the world economy. controlling swine diseases in the farm is essential in order to achieve the sector necessities. aujeszky's disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the swine industry. micrornas (mirnas) are non-coding rnas which modulates gene expression in animals, plants and viruses. with the aim of understanding mirna roles during the aujeszky's disease virus [ad ... | 2014 | 24475202 |
| lateral geniculate body evoked potentials elicited by visual and electrical stimulation. | blind individuals who have photoreceptor loss are known to perceive phosphenes with electrical stimulation of their remaining retinal ganglion cells. we proposed that implantable lateral geniculate body (lgb) stimulus electrode arrays could be used to generate phosphene vision. we attempted to refine the basic reference of the electrical evoked potentials (eeps) elicited by microelectrical stimulations of the optic nerve, optic tract and lgb of a domestic pig, and then compared it to visual evok ... | 2014 | 25120343 |
| genetic structure of wild boar (sus scrofa) populations from east asia based on microsatellite loci analyses. | wild boar, sus scrofa, is an extant wild ancestor of the domestic pig as an agro-economically important mammal. wild boar has a worldwide distribution with its geographic origin in southeast asia, but genetic diversity and genetic structure of wild boar in east asia are poorly understood. to characterize the pattern and amount of genetic variation and population structure of wild boar in east asia, we genotyped and analyzed microsatellite loci for a total of 238 wild boar specimens from ten loca ... | 2014 | 25034725 |
| genomic analysis reveals selection for asian genes in european pigs following human-mediated introgression. | the independent domestication of local wild boar populations in asia and europe about 10,000 years ago led to distinct european and asian pig breeds, each with very different phenotypic characteristics. during the industrial revolution, chinese breeds were imported to europe to improve commercial traits in european breeds. here we demonstrate the presence of introgressed asian haplotypes in european domestic pigs and selection signatures on some loci in these regions, using whole genome sequence ... | 2014 | 25025832 |
| stereological estimation of total cell numbers in the human cerebral and cerebellar cortex. | our knowledge of the relationship between brain structure and cognitive function is still limited. human brains and individual cortical areas vary considerably in size and shape. studies of brain cell numbers have historically been based on biased methods, which did not always result in correct estimates and were often very time-consuming. within the last 20-30 years, it has become possible to rely on more advanced and unbiased methods. these methods have provided us with information about fetal ... | 2014 | 25076882 |
| complete genome sequences of two central european brucella suis bv. 2 haplotype 2c strains isolated from wild boars. | the brucella suis haplotype 2c is commonly isolated from wild boars and domestic pigs across central europe, though it is rarely described in the iberian peninsula. we report here the complete and annotated genome sequences of two haplotype 2c strains isolated from wild boars in the northeast region of spain, above the ebro river. | 2014 | 25013144 |
| design and development of exome capture sequencing for the domestic pig (sus scrofa). | the domestic pig (sus scrofa) is both an important livestock species and a model for biomedical research. exome sequencing has accelerated identification of protein-coding variants underlying phenotypic traits in human and mouse. we aimed to develop and validate a similar resource for the pig. | 2014 | 24988888 |
| meat species identification using loop-mediated isothermal amplification assay targeting species-specific mitochondrial dna. | meat source fraud and adulteration scandals have led to consumer demands for accurate meat identification methods. nucleotide amplification assays have been proposed as an alternative method to protein-based assays for meat identification. in this study, we designed loop-mediated isothermal amplification (lamp) assays targeting species-specific mitochondrial dna to identify and discriminate eight meat species; cattle, pig, horse, goat, sheep, chicken, duck, and turkey. the lamp primer sets were ... | 2014 | 26761677 |
| high-resolution melting of 12s rrna and cytochrome b dna sequences for discrimination of species within distinct european animal families. | the cheap and easy identification of species is necessary within multiple fields of molecular biology. the use of high-resolution melting (hrm) of dna provides a fast closed-tube method for analysis of the sequence composition of the mitochondrial genes 12s rrna and cytochrome b. we investigated the potential use of hrm for species identification within eleven different animal groups commonly found in europe by animal-group-specific dna amplification followed by dna melting. influence factors as ... | 2014 | 25531439 |
| utility of cell viability assays for use with ex vivo vocal fold epithelial tissue. | ex vivo models are routinely used to investigate the barrier function of the vocal fold epithelium. however, there are limited reports on assays that can be used to investigate the effect of clinically relevant challenges on vocal fold epithelial tissue viability. our objective was to determine the utility of two assays routinely used in cell culture-a cellular metabolic activity assay and a cell membrane integrity assay-to investigate the viability of ex vivo porcine vocal fold epithelium. | 2014 | 25511412 |
| activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia. | notochordal cells (nc) remain in the focus of research for regenerative therapy for the degenerated intervertebral disc (ivd) due to their progenitor status. recent findings suggested their regenerative action on more mature disc cells, presumably by the secretion of specific factors, which has been described as notochordal cell conditioned medium (nccm). the aim of this study was to determine nc culture conditions (2d/3d, fetal calf serum, oxygen level) that lead to significant ivd cell activat ... | 2014 | 25496082 |
| hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs. | preliminary studies showed the prevalence of a virus similar to human hepatitis b virus (hbv-like) in swine from farms in china and the molecular evidence of hepadnavirus infection in domestic pigs herds in brazil. in this study, we genetically characterize the swine hepadnavirus strains in swine from slaughterhouses located in certified abattoirs from rio de janeiro state, brazil and evaluate its hepatotropic potential. | 2014 | 25495746 |
| biomechanical evaluation of three fixation modalities for preperitoneal inguinal hernia repair: a 24-hour postoperative study in pigs. | tacks and sutures ensure a strong fixation of meshes, but they can be associated with pain and discomfort. less invasive methods are now available. three fixation modalities were compared: the progrip™ laparoscopic self-fixating mesh; the fibrin glue tisseel™ with bard™ soft mesh; and the sorbafix™ absorbable fixation system with bard™ soft mesh. | 2014 | 25525396 |
| a novel strain of porcine adenovirus detected in urinary bladder urothelial cell culture. | contamination of cell cultures is the most common problem encountered in cell culture laboratories. besides the secondary cell contaminations often occurring in the cell laboratories, the contaminations originating from donor animal or human tissue are equally as common, but usually harder to recognize and as such require special attention. the present study describes the detection of porcine adenovirus (padv), strain padv-svn1 in cultures of normal porcine urothelial (npu) cells isolated from u ... | 2014 | 24960273 |
| targeting the ion channel kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-life. | ion channels are an attractive class of drug targets, but progress in developing inhibitors for therapeutic use has been limited largely due to challenges in identifying subtype selective small molecules. animal venoms provide an alternative source of ion channel modulators, and the venoms of several species, such as scorpions, spiders and snails, are known to be rich sources of ion channel modulating peptides. importantly, these peptides often bind to hyper-variable extracellular loops, creatin ... | 2014 | 24939846 |
| untangling the hybrid nature of modern pig genomes: a mosaic derived from biogeographically distinct and highly divergent sus scrofa populations. | the merging of populations after an extended period of isolation and divergence is a common phenomenon, in natural settings as well as due to human interference. individuals with such hybrid origins contain genomes that essentially form a mosaic of different histories and demographies. pigs are an excellent model species to study hybridization because european and asian wild boars diverged ~1.2 mya, and pigs were domesticated independently in europe and asia. during the industrial revolution in ... | 2014 | 24863459 |
| meiotic recombination analyses of individual chromosomes in male domestic pigs (sus scrofa domestica). | for the first time in the domestic pig, meiotic recombination along the 18 porcine autosomes was directly studied by immunolocalization of mlh1 protein. in total, 7,848 synaptonemal complexes from 436 spermatocytes were analyzed, and 13,969 recombination sites were mapped. individual chromosomes for 113 of the 436 cells (representing 2,034 synaptonemal complexes) were identified by immunostaining and fluorescence in situ hybridization (fish). the average total length of autosomal synaptonemal co ... | 2014 | 24919066 |
| genome-wide identification of long intergenic noncoding rna genes and their potential association with domestication in pigs. | thousands of long intergenic noncoding rnas (lincrnas) have been identified in the human and mouse genomes, some of which play important roles in fundamental biological processes. the pig is an important domesticated animal, however, pig lincrnas remain poorly characterized and it is unknown if they were involved in the domestication of the pig. here, we used available rna-seq resources derived from 93 samples and expressed sequence tag data sets, and identified 6,621 lincrna transcripts from 4, ... | 2014 | 24891613 |
| using biplanar fluoroscopy to guide radiopaque vascular injections: a new method for vascular imaging. | studying vascular anatomy, especially in the context of relationships with hard tissues, is of great interest to biologists. vascular studies have provided significant insight into physiology, function, phylogenetic relationships, and evolutionary patterns. injection of resin or latex into the vascular system has been a standard technique for decades. there has been a recent surge in popularity of more modern methods, especially radiopaque latex vascular injection followed by ct scanning and dig ... | 2014 | 24835249 |
| repeated evolution of chimeric fusion genes in the β-globin gene family of laurasiatherian mammals. | the evolutionary fate of chimeric fusion genes may be strongly influenced by their recombinational mode of origin and the nature of functional divergence between the parental genes. in the β-globin gene family of placental mammals, the two postnatally expressed δ- and β-globin genes (hbd and hbb, respectively) have a propensity for recombinational exchange via gene conversion and unequal crossing-over. in the latter case, there are good reasons to expect differences in retention rates for the re ... | 2014 | 24814285 |
| comparison of the decomposition voc profile during winter and summer in a moist, mid-latitude (cfb) climate. | the investigation of volatile organic compounds (vocs) associated with decomposition is an emerging field in forensic taphonomy due to their importance in locating human remains using biological detectors such as insects and canines. a consistent decomposition voc profile has not yet been elucidated due to the intrinsic impact of the environment on the decomposition process in different climatic zones. the study of decomposition vocs has typically occurred during the warmer months to enable chem ... | 2014 | 25412504 |
| bupivacaine mandibular nerve block affects intraoperative blood pressure and heart rate in a yucatan miniature swine mandibular condylectomy model: a pilot study. | the primary objective was to evaluate the effect of a bupivacaine mandibular nerve block on intraoperative blood pressure (bp) and heart rate (hr) in response to surgical stimulation and the need for systemic analgesics postoperatively. we hypothesized that a mandibular nerve block would decrease the need for systemic analgesics both intraoperatively and postoperatively. | 2014 | 25394295 |
| cellular scaling rules for the brain of artiodactyla include a highly folded cortex with few neurons. | quantitative analysis of the cellular composition of rodent, primate, insectivore, and afrotherian brains has shown that non-neuronal scaling rules are similar across these mammalian orders that diverged about 95 million years ago, and therefore appear to be conserved in evolution, while neuronal scaling rules appear to be free to vary in a clade-specific manner. here we analyze the cellular scaling rules that apply to the brain of artiodactyls, a group within the order cetartiodactyla, believed ... | 2014 | 25429261 |
| endothelin-a receptor antagonism after renal angioplasty enhances renal recovery in renovascular disease. | percutaneous transluminal renal angioplasty/stenting (ptras) is frequently used to treat renal artery stenosis and renovascular disease (rvd); however, renal function is restored in less than one half of the cases. this study was designed to test a novel intervention that could refine ptras and enhance renal recovery in rvd. renal function was quantified in pigs after 6 weeks of chronic rvd (induced by unilateral renal artery stenosis), established renal damage, and hypertension. pigs with rvd t ... | 2014 | 25377076 |
| ovine β-lactoglobulin at atomic resolution. | the crystal structure of the triclinic form of the milk protein β-lactoglobulin from sheep (ovis aries) at 1.1 å resolution is described together with a comparison of the triclinic structures of the low-ph bovine and high-ph ovine proteins. all three structures are remarkably similar, despite the well known ph-dependent conformational transition described for the bovine and porcine proteins that occurs in solution. the high resolution of the present structure determination has allowed a more acc ... | 2014 | 25372816 |
| immune modulation in xenotransplantation. | the use of animals as donors of tissues and organs for xenotransplantations may help in meeting the increasing demand for organs for human transplantations. clinical studies indicate that the domestic pig best satisfies the criteria of organ suitability for xenotransplantation. however, the considerable phylogenetic distance between humans and the pig causes tremendous immunological problems after transplantation, thus genetic modifications need to be introduced to the porcine genome, with the a ... | 2014 | 25354539 |
| a genome-wide scan for selection signatures in yorkshire and landrace pigs based on sequencing data. | pigs have experienced dramatic selection due to domestication, which has led to many different phenotypes when compared to their wild counterparts, especially in the last several decades. currently, genome-wide scans in both cattle and humans showing positive selection footprints have been investigated. however, few studies have focused on porcine selection footprints, particularly on a genome-wide scale. surveying for selection footprints across porcine genomes can be quite valuable for reveali ... | 2014 | 25327778 |
| serological evidence for influenza virus infection in korean wild boars. | serum samples from 1,011 wild boars hunted in 2012 were collected for serological surveillance for 4 subtypes (pandemic a (h1n1) 2009 and classical h1n1, h1n2 and h3n2) of swine influenza virus (siv). samples from 12 of the boars were identified as positive for siv (pandemic a (h1n1) 2009, n=9; classical h1n1, n=2; and h1n2, n=1) by a hemagglutination inhibition test (hi test) and a nucleoprotein (np)-based enzyme-linked immunosorbent assay (np-elisa). although the overall seroprevalence of siv ... | 2014 | 25298239 |
| catheter-based induction of renal ischemia/reperfusion in swine: description of an experimental model. | several techniques to induce renal ischemia have been proposed: clamp, pva particles, and catheter-balloon. we report the development of a controlled, single-insult model of unilateral renal ischemia/reperfusion (i/r) without contralateral nephrectomy, using a suitable model, the pig. this is a balloon-catheter-based model using a percutaneous, interventional radiology procedure. one angioplasty balloon-catheter was placed into the right renal artery and inflated for 120 min and reperfusion over ... | 2014 | 25263203 |
| histologic characterization of acellular dermal matrices in a porcine model of tissue expander breast reconstruction. | acellular dermal matrices (adms) have been commonly used in expander-based breast reconstruction to provide inferolateral prosthesis coverage. although the clinical performance of these biologic scaffold materials varies depending on a number of factors, an in-depth systematic characterization of the host response is yet to be performed. the present study evaluates the biochemical composition and structure of two adms, alloderm(®) regenerative tissue matrix and allomax™ surgical graft, and provi ... | 2014 | 24941900 |
| dynamics of african swine fever virus shedding and excretion in domestic pigs infected by intramuscular inoculation and contact transmission. | african swine fever virus (asfv) is a highly virulent swine pathogen that has spread across eastern europe since 2007 and for which there is no effective vaccine or treatment available. the dynamics of shedding and excretion is not well known for this currently circulating asfv strain. therefore, susceptible pigs were exposed to pigs intramuscularly infected with the georgia 2007/1 asfv strain to measure those dynamics through within- and between-pen transmission scenarios. blood, oral, nasal an ... | 2014 | 25256695 |
| an in vivo three-dimensional magnetic resonance imaging-based averaged brain collection of the neonatal piglet (sus scrofa). | due to the fact that morphology and perinatal growth of the piglet brain is similar to humans, use of the piglet as a translational animal model for neurodevelopmental studies is increasing. magnetic resonance imaging (mri) can be a powerful tool to study neurodevelopment in piglets, but many of the mri resources have been produced for adult humans. here, we present an average in vivo mri-based atlas specific for the 4-week-old piglet. in addition, we have developed probabilistic tissue classifi ... | 2014 | 25254955 |
| indirect genetic effects for growth rate in domestic pigs alter aggressive and manipulative biting behaviour. | indirect genetic effects (iges) are heritable effects of an individual on phenotypic values of others, and may result from social interactions. we determined the behavioural consequences of selection for iges for growth (igeg) in pigs in a g × e treatment design. pigs (n = 480) were selected for high versus low igeg with a contrast of 14 g average daily gain and were housed in either barren or straw-enriched pens (n = 80). high igeg pigs showed from 8 to 23 weeks age 40% less aggressive biting ( ... | 2014 | 25227986 |
| low tidal volume pressure support versus controlled ventilation in early experimental sepsis in pigs. | in moderate acute respiratory distress syndrome (ards) several studies support the usage of assisted spontaneous breathing modes. only limited data, however, focus on the application in systemic sepsis and developing lung injury. the present study examines the effects of immediate initiation of pressure support ventilation (psv) in a model of sepsis-induced ards. | 2014 | 25189285 |
| development of a closed-artery catheter-based myocardial infarction in pigs using sponge and lidocaine hydrochloride infusion to prevent irreversible ventricular fibrillation. | the objectives of this study were to develop a robust, homogeneous, viable and inexpensive model of closed-artery catheter-based model of myocardial infarction (mi) in pigs without major cardiac dysfunction. suitable animal models that mimic human cardiovascular conditions are of paramount importance to understand the effects of novel therapeutic strategies to improve tissue perfusion and prevent cardiac deterioration post-mi. pigs (n = 21, bw = 17 ± 1 kg) receiving continuous iv lidocaine hydro ... | 2014 | 25168871 |
| crossover of the hypothalamic pituitary-adrenal/interrenal, -thyroid, and -gonadal axes in testicular development. | besides the well-known function of thyroid hormones (ths) for regulating metabolism, it has recently been discovered that ths are also involved in testicular development in mammalian and non-mammalian species. ths, in combination with follicle stimulating hormone, lead to androgen synthesis in danio rerio, which results in the onset of spermatogenesis in the testis, potentially relating the hypothalamic-pituitary-thyroid (hpt) gland to the hypothalamic-pituitary-gonadal (hpg) axes. furthermore, ... | 2014 | 25221542 |
| xenografting of testicular tissue pieces: 12 years of an in vivo spermatogenesis system. | spermatogenesis is a dynamic and complex process that involves endocrine and testicular factors. during xenotransplantation of testicular tissue fragments into immunodecifient mice, a functional communication between host brain and donor testis is established. this interaction allows for the progression of spermatogenesis and recovery of fertilisation-competent spermatozoa from a broad range of mammalian species. in the last few years, significant progress has been achieved in testis tissue xeno ... | 2014 | 25150043 |
| differential axonal conduction patterns of mechano-sensitive and mechano-insensitive nociceptors--a combined experimental and modelling study. | cutaneous pain sensations are mediated largely by c-nociceptors consisting of both mechano-sensitive (cm) and mechano-insensitive (cmi) fibres that can be distinguished from one another according to their characteristic axonal properties. in healthy skin and relative to cmi fibres, cm fibres show a higher initial conduction velocity, less activity-dependent conduction velocity slowing, and less prominent post-spike supernormality. however, after sensitization with nerve growth factor, the electr ... | 2014 | 25136824 |
| microscopy and genomic analysis of mycoplasma parvum strain indiana. | mycoplasma parvum [eperythrozoon parvum] is the second hemotrophic mycoplasma (hemoplasma) described in pigs. unlike m. suis, its closest phylogenetic relative, m. parvum, is considered a non-pathogenic bacterium in this host species. natural infection of a domestic, 6-month-old splenectomized pig with m. parvum strain indiana is described herein. light and scanning electron microscopy of the bacteria were performed in addition to whole genome sequencing, analysis, and comparison to the genome o ... | 2014 | 25113534 |
| scabies mites alter the skin microbiome and promote growth of opportunistic pathogens in a porcine model. | the resident skin microbiota plays an important role in restricting pathogenic bacteria, thereby protecting the host. scabies mites (sarcoptes scabiei) are thought to promote bacterial infections by breaching the skin barrier and excreting molecules that inhibit host innate immune responses. epidemiological studies in humans confirm increased incidence of impetigo, generally caused by staphylococcus aureus and streptococcus pyogenes, secondary to the epidermal infestation with the parasitic mite ... | 2014 | 24875186 |
| the relationship between parasite fitness and host condition in an insect--virus system. | research in host-parasite evolutionary ecology has demonstrated that environmental variation plays a large role in mediating the outcome of parasite infection. for example, crowding or low food availability can reduce host condition and make them more vulnerable to parasite infection. this observation that poor-condition hosts often suffer more from parasite infection compared to healthy hosts has led to the assumption that parasite productivity is higher in poor-condition hosts. however, the ub ... | 2014 | 25208329 |
| fine-mapping qtls in advanced intercross lines and other outbred populations. | quantitative genetic studies in model organisms, particularly in mice, have been extremely successful in identifying chromosomal regions that are associated with a wide variety of behavioral and other traits. however, it is now widely understood that identification of the underlying genes will be far more challenging. in the last few years, a variety of populations have been utilized in an effort to more finely map these chromosomal regions with the goal of identifying specific genes. the common ... | 2014 | 24906874 |
| molecular characterization of african swine fever virus from domestic pigs in northern tanzania during an outbreak in 2013. | african swine fever (asf) is an acute, highly contagious and deadly viral hemorrhagic fever of domestic pigs caused by african swine fever virus (asfv), a double-stranded dna virus of the family asfarviridae. in this study, molecular diagnosis and characterization of outbreak asfv in northern tanzania, was performed on spleen, lymph node, kidney, and heart samples collected in june and july 2013 from domestic pigs that died during a hemorrhagic disease outbreak. confirmatory diagnosis of asf was ... | 2014 | 24996815 |
| spatial multi-criteria decision analysis to predict suitability for african swine fever endemicity in africa. | african swine fever (asf) is endemic in several countries of africa and may pose a risk to all pig producing areas on the continent. official asf reporting is often rare and there remains limited awareness of the continent-wide distribution of the disease.in the absence of accurate asf outbreak data and few quantitative studies on the epidemiology of the disease in africa, we used spatial multi-criteria decision analysis (mcda) to derive predictions of the continental distribution of suitability ... | 2014 | 24406022 |
| effect of gaseous ozone exposure on the bacteria counts and oxidative properties of ground hanwoo beef at refrigeration temperature. | this study was designed to elucidate the effect of ozone exposure on the bacteria counts and oxidative properties of ground hanwoo beef contaminated with escherichia coli o157:h7 at refrigeration temperature. ground beef was inoculated with 7 log cfu/g of e. coli o157:h7 isolated from domestic pigs and was then subjected to ozone exposure (10×10(-6) kg o3 h(-1)) at 4℃ for 3 d. e. coli o157:h7, total aerobic and anaerobic bacterial growth and oxidative properties including instrumental color chan ... | 2014 | 26761291 |
| decomposition odour profiling in the air and soil surrounding vertebrate carrion. | chemical profiling of decomposition odour is conducted in the environmental sciences to detect malodourous target sources in air, water or soil. more recently decomposition odour profiling has been employed in the forensic sciences to generate a profile of the volatile organic compounds (vocs) produced by decomposed remains. the chemical profile of decomposition odour is still being debated with variations in the voc profile attributed to the sample collection technique, method of chemical analy ... | 2014 | 24740412 |
| identification of blood meal sources of lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome b gene. | an analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. the present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis of the mitochondrial cytochrome b (cytb) gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. the complete cytb gene sequences of 11 vertebrate species available in ... | 2014 | 24821056 |
| a quantitative prioritisation of human and domestic animal pathogens in europe. | disease or pathogen risk prioritisations aid understanding of infectious agent impact within surveillance or mitigation and biosecurity work, but take significant development. previous work has shown the h-(hirsch-)index as an alternative proxy. we present a weighted risk analysis describing infectious pathogen impact for human health (human pathogens) and well-being (domestic animal pathogens) using an objective, evidence-based, repeatable approach; the h-index. this study established the highe ... | 2014 | 25136810 |
| epidemiology of pathogenic enterobacteria in humans, livestock, and peridomestic rodents in rural madagascar. | among the families of enteric bacteria are globally important diarrheal agents. despite their potential for zoonotic and environmental transmission, few studies have examined the epidemiology of these pathogens in rural systems characterized by extensive overlap among humans, domesticated and peridomestic animals. we investigated patterns of infection with enterotoxigenic escherichia coli, shigella spp., salmonella enterica, vibrio cholerae, and yersinia spp. (enterocolitica, and pseudotuberculo ... | 2014 | 24983990 |
| characterisation of the active/de-active transition of mitochondrial complex i. | oxidation of nadh in the mitochondrial matrix of aerobic cells is catalysed by mitochondrial complex i. the regulation of this mitochondrial enzyme is not completely understood. an interesting characteristic of complex i from some organisms is the ability to adopt two distinct states: the so-called catalytically active (a) and the de-active, dormant state (d). the a-form in situ can undergo de-activation when the activity of the respiratory chain is limited (i.e. in the absence of oxygen). the m ... | 2014 | 24569053 |
| first report of corynebacterium pseudotuberculosis from caseous lymphadenitis lesions in black alentejano pig (sus scrofa domesticus). | corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis, a common disease in small ruminant populations throughout the world and responsible for a significant economic impact for producers. | 2014 | 25240511 |
| direct detection and differentiation of pathogenic leptospira species using a multi-gene targeted real time pcr approach. | leptospirosis is a growing public and veterinary health concern caused by pathogenic species of leptospira. rapid and reliable laboratory tests for the direct detection of leptospiral infections in animals are in high demand not only to improve diagnosis but also for understanding the epidemiology of the disease. in this work we describe a novel and simple taqman-based multi-gene targeted real-time pcr approach able to detect and differentiate leptospira interrogans, l. kirschneri, l. borgpetere ... | 2014 | 25398140 |
| 'black sheep' that don't leave the double-stranded rna-binding domain fold. | the canonical double-stranded rna (dsrna)-binding domain (dsrbd) is composed of an α1-β1-β2-β3-α2 secondary structure that folds in three dimensions to recognize dsrna. recently, structural and functional studies of divergent dsrbds revealed adaptations that include intra- and/or intermolecular protein interactions, sometimes in the absence of detectable dsrna-binding ability. we describe here how discrete dsrbd components can accommodate pronounced amino-acid sequence changes while maintaining ... | 2014 | 24954387 |
| genomic analysis of influenza a virus from captive wild boars in brazil reveals a human-like h1n2 influenza virus. | influenza is a viral disease that affects human and several animal species. in brazil, h1n1, h3n2 and 2009 pandemic h1n1 a(h1n1)pdm09 influenza a viruses (iav) circulate in domestic swine herds. wild boars are also susceptible to iav infection but in brazil until this moment there are no reports of iav infection in wild boars or in captive wild boars populations. herein the occurrence of iav in captive wild boars with the presence of lung consolidation lesions during slaughter was investigated. ... | 2014 | 24238665 |
| shiga toxin-producing escherichia coli in swine: the public health perspective. | shiga toxin-producing escherichia coli (stec) strains are food-borne pathogens that are an important public health concern. stec infection is associated with severe clinical diseases in human beings, including hemorrhagic colitis (hc) and hemolytic uremic syndrome (hus), which can lead to kidney failure and death. cattle are the most important stec reservoir. however, a number of stec outbreaks and hus cases have been attributed to pork products. in swine, stec strains are known to be associated ... | 2014 | 24397985 |
| a serological survey for antibodies against foot-and-mouth disease virus (fmdv) in domestic pigs during outbreaks in kenya. | foot-and-mouth disease (fmd) is endemic in kenya and has been well studied in cattle, but not in pigs, yet the role of pigs is recognised in fmd-free areas. this study investigated the presence of antibodies against fmd virus (fmdv) in pigs sampled during a countrywide random survey for fmd in cattle coinciding with sat 1 fmdv outbreaks in cattle. a total of 191 serum samples were collected from clinically healthy pigs in 17 districts. forty-two of the 191 sera were from pigs vaccinated against ... | 2014 | 24442573 |
| prevalence and diversity of encephalitozoon spp. and enterocytozoon bieneusi in wild boars (sus scrofa) in central europe. | from 2011 to 2012, the occurrence of enterocytozoon bieneusi and encephalitozoon spp. was surveyed at 29 randomly selected localities (both forest areas and enclosures) across four central european countries: austria, the czech republic, poland, and the slovak republic. isolates were genotyped by pcr amplification and characterization of the internal transcribed spacer (its) region using enterocytozoon and encephalitozoon-specific protocols. pcr revealed 16 mono-infections of encephalitozoon cun ... | 2014 | 24292543 |