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the mixture of cashew nut shell liquid and castor oil results in an efficient larvicide against aedes aegypti that does not alter embryo-fetal development, reproductive performance or dna integrity.dengue fever, chikungunya fever and zika virus are epidemics in brazil that are transmitted by mosquitoes, such as aedes aegypti or aedes albopictus. the liquid from shells of cashew nuts is attractive for its important biological and therapeutic activities, which include toxicity to mosquitoes of the genus aedes. the present study evaluated the effects of a mixture of surfactants from natural cashew nutshell liquid and castor oil (named talcc-20) on the mortality of larvae and on the reproducti ...201829554095
mapping the virome in wild-caught aedes aegypti from cairns and bangkok.medically important arboviruses such as dengue, zika, and chikungunya viruses are primarily transmitted by the globally distributed mosquito aedes aegypti. increasing evidence suggests that transmission can be influenced by mosquito viromes. herein rna-seq was used to characterize rna metaviromes of wild-caught ae. aegypti from bangkok (thailand) and from cairns (australia). the two mosquito populations showed a high degree of similarity in their viromes. blast searches of assembled contigs sugg ...201829549363
estimating the probability of dengue virus introduction and secondary autochthonous cases in europe.given the speed of air travel, diseases even with a short viremia such as dengue can be easily exported to dengue naïve areas within 24 hours. we set out to estimate the risk of dengue virus introductions via travelers into europe and number of secondary autochthonous cases as a result of the introduction. we applied mathematical modeling to estimate the number of dengue-viremic air passengers from 16 dengue-endemic countries to 27 european countries, taking into account the incidence of dengue ...201829545610
house screening with insecticide-treated netting provides sustained reductions in domestic populations of aedes aegypti in merida, mexico.there is a need for effective methods to control aedes aegypti and prevent the transmission of dengue, chikungunya, yellow fever and zika viruses. insecticide treated screening (its) is a promising approach, particularly as it targets adult mosquitoes to reduce human-mosquito contact.201829543805
the seasonal influence of climate and environment on yellow fever transmission across africa.yellow fever virus (yfv) is a vector-borne flavivirus endemic to africa and latin america. ninety per cent of the global burden occurs in africa where it is primarily transmitted by aedes spp, with aedes aegypti the main vector for urban yellow fever (yf). mosquito life cycle and viral replication in the mosquito are heavily dependent on climate, particularly temperature and rainfall. we aimed to assess whether seasonal variations in climatic factors are associated with the seasonality of yf rep ...201829543798
risk perception and level of knowledge of diseases transmitted by aedes aegypti.diseases caused by viruses such as dengue, chikungunya and zika are mosquito-borne diseases transmitted by aedes aegypti. we performed a cross-sectional study of healthcare personnel and the general population using questionnaires to identify the level of knowledge, attitudes and practices, and risk perception for dengue, chikungunya and zika. a total of 248 questionnaires were applied, 63.3% to healthcare personnel and 36.7% to the general population. of the healthcare personnel, 53% were men, ...201829538507
dengue virus in aedes aegypti and aedes albopictus in urban areas in the state of rio grande do norte, brazil: importance of virological and entomological surveillance.vector control remains the sole effective method to prevent dengue virus (denv) transmission, although a vaccine for dengue has recently become available and testing of its efficacy and coverage is being performed in multiple places. entomological surveillance is a key factor in alerting authorities to possible outbreaks, but until now natural denv infection of mosquito populations has been scarcely used as an early warning system to monitor fluctuating prevalence of infected mosquitoes. the pur ...201829534105
chikungunya virus: a rheumatologist's perspective.chikungunya virus (chikv) is an arthropod-borne alphavirus, transmitted by aedes aegypti and aedes albopictus mosquitoes. it is responsible for a febrile illness, typically accompanied by maculopapular rash and severe, incapacitating arthralgia. the disease, although generally self-limiting, frequently evolves into a long-lasting, debilitating rheumatic disorder, which shares many clinical features with rheumatoid arthritis (ra). the underlying mechanism by which chikv induces persistent arthrit ...201829533749
comparative phylogeography ofaedesmosquitoes and the role of past climatic change for evolution within africa.the study of demographic processes involved in species diversification and evolution ultimately provides explanations for the complex distribution of biodiversity on earth, indicates regions important for the maintenance and generation of biodiversity, and identifies biological units important for conservation or medical consequence. african and forest biota have both received relatively little attention with regard to understanding their diversification, although one possible mechanism is that ...201829531714
natural variation in resistance to virus infection in dipteran insects.the power and ease ofdrosophilagenetics and the medical relevance of mosquito-transmitted viruses have made dipterans important model organisms in antiviral immunology. studies of virus-host interactions at the molecular and population levels have illuminated determinants of resistance to virus infection. here, we review the sources and nature of variation in antiviral immunity and virus susceptibility in model dipteran insects, specifically the fruit flydrosophila melanogasterand vector mosquit ...201829522475
update: noncongenital zika virus disease cases - 50 u.s. states and the district of columbia, 2016.zika virus is a flavivirus primarily transmitted to humans by aedes aegypti mosquitoes (1). zika virus infections also have been documented through intrauterine transmission resulting in congenital infection; intrapartum transmission from a viremic mother to her newborn; sexual transmission; blood transfusion; and laboratory exposure (1-3). most zika virus infections are asymptomatic or result in mild clinical illness, characterized by acute onset of fever, maculopapular rash, arthralgia, or non ...201829518067
impacts of diurnal temperature and larval density on aquatic development of aedes aegypti.the increasing range of aedes aegypti, vector for zika, dengue, chikungunya, and other viruses, has brought attention to the need to understand the population and transmission dynamics of this mosquito. it is well understood that environmental factors and breeding site characteristics play a role in organismal development and the potential to transmit pathogens. in this study, we observe the impact of larval density in combination with diurnal temperature on the time to pupation, emergence, and ...201829513751
zika virus epidemiology in bolivia: a seroprevalence study in volunteer blood donors.zika virus (zikv), was widely reported in latin america and has been associated with neuropathologies, as microcephaly, but only few seroprevalence studies have been published to date. our objective was to determine the seroprevalence amongst bolivian blood donors and estimate the future potential circulation of the virus.201829513667
swift fabrication of ag nanostructures using a colloidal solution of holostemma ada-kodien (apocynaceae) - antibiofilm potential, insecticidal activity against mosquitoes and non-target impact on water bugs.recent research in entomology and parasitology focused on the efficacy of green fabricated nanomaterials as novel insecticides. in this study, we synthesized poly-dispersed and stable silver nanoparticles (agnps) using the leaf extract of holostemma ada-kodien. the nanostructures were characterized by ultraviolet-visible spectroscopy, fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive x-ray, and x-ray diffraction analysis. t ...201829510358
salivary factor ltrin from aedes aegypti facilitates the transmission of zika virus by interfering with the lymphotoxin-β receptor.pathogens have co-evolved with mosquitoes to optimize transmission to hosts. mosquito salivary-gland extract is known to modulate host immune responses and facilitate pathogen transmission, but the underlying molecular mechanisms of this have remained unknown. in this study, we identified and characterized a prominent 15-kilodalton protein, ltrin, obtained from the salivary glands of the mosquito aedes aegypti. ltrin expression was upregulated in blood-fed mosquitoes, and ltrin facilitated the t ...201829507355
transovarial transmission of dengue 1 virus in aedes aegypti larvae: real-time pcr analysis in a brazilian city with high mosquito population density.transovarial transmission is among the reported factors able to influence environmental maintenance of dengue virus (denv). endemic areas with active transmission of dengue are suitable for studying transovarial transmission. brazil is a country where dengue is endemic and where denv-1 is the most common disease-related virus serotype. this study aimed to identify transovarial transmission of denv-1 in aedes aegypti larvae by reverse-transcriptase nested real-time polymerase chain reaction. betw ...201829505732
increased adult aedes aegypti and culex quinquefasciatus (diptera: culicidae) abundance in a dengue transmission hotspot, compared to a coldspot, within kaohsiung city, taiwan.the assumption that vector abundance differences might drive spatial and temporal heterogeneities in vector-borne disease transmission is common, though data supporting it is scarce. here, we present data from two common mosquito species aedes aegypti (linnaeus) and culex quinquefasciatus say, biweekly sampled as adults, from march 2016 through december 2017, with bg-sentinel traps in two neighboring districts of kaohsiung city (kc), taiwan. one district has historically been a dengue transmissi ...201830104501
impact of mosquito age and insecticide exposure on susceptibility of aedes albopictus (diptera: culicidae) to infection with zika virus.zika virus (zikv) is primarily transmitted to humans by aedes aegypti and ae. albopictus. vector⁻virus interactions influencing vector competence vary and depend on biological and environmental factors. a mosquito's chronological age may impact its immune response against virus infection. insecticides, source reduction, and/or public education are currently the best defense against mosquitoes that transmit zikv. this study assessed the effects of a mosquito's chronological age at time of infecti ...201830103526
differences in the replicative capacities of clinical isolates of dengue virus in c6/36 cells and in urban populations of aedes aegypti from colombia, south america.dengue, the most prevalent arboviral disease worldwide, is caused by any of the four dengue virus (denv) serotypes that co-circulate constantly in hyperendemic areas such as medellin (colombia), and these serotypes are transmitted by mosquitoes of the genus aedes. in this study, we evaluated the replicative capacity of strains isolated in medellin between 2003 and 2007 in c6/36 cells and in colonies of aedes aegypti collected during 2010-2011 from high or low-incidence areas within the same city ...201830165044
direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of wolbachia using a cellphone.manipulation of natural mosquito populations using the endosymbiotic bacteria wolbachia is being investigated as a novel strategy to reduce the burden of mosquito-borne viruses. to evaluate the efficacy of these interventions, it will be critical to determine wolbachia infection frequencies in aedes aegypti mosquito populations. however, current diagnostic tools are not well-suited to fit this need. morphological methods cannot identify wolbachia, immunoassays often suffer from low sensitivity a ...201830161131
the surveillance of chikungunya virus in a temperate climate: challenges and possible solutions from the experience of lazio region, italy.chikv has become an emerging public health concern in the temperate regions of the northern hemisphere as a consequenceof the expansion of the endemic areas of its vectors (mainly aedes aegypti and aedesalbopictus). in 2017, a new outbreak of chikv was detected in italy with three clusters of autochthonous transmission in the lazio region (central italy), in the cities of anzio, rome, and latina and a secondary cluster in the calabria region (south italy). given the climate characteristics of it ...201830223536
where vectors collide: the importance of mechanisms shaping the realized niche for modeling ranges of invasive aedes mosquitoes.the vector mosquitoes aedes aegypti (l.), native to africa, and aedes albopictus (skuse), native to asia, are widespread invasives whose spatial distributions frequently overlap. predictive models of their distributions are typically correlative rather than mechanistic, and based on only abiotic variables describing putative environmental requirements despite extensive evidence of competitive interactions leading to displacements. here we review putative roles of competition contributing to dist ...201830220875
regulation of midgut cell proliferation impacts aedes aegypti susceptibility to dengue virus.aedes aegypti is the vector of some of the most important vector-borne diseases like dengue, chikungunya, zika and yellow fever, affecting millions of people worldwide. the cellular processes that follow a blood meal in the mosquito midgut are directly associated with pathogen transmission. we studied the homeostatic response of the midgut against oxidative stress, as well as bacterial and dengue virus (denv) infections, focusing on the proliferative ability of the intestinal stem cells (isc). i ...201829782512
zika virus: report from the task force on tropical diseases by the world federation of societies of intensive and critical care medicine.zika fever is an acute infectious disease caused by the zika virus (zikv), an rna virus belonging to the genus flavivirus. zikv can be transmitted by bites from aedes aegypti and aedes albopictus species, sexual activity, blood transfusion and from mother to fetus during pregnancy and delivery. a total of 50 territories and countries in the americas had reported zikv infections. for those with symptoms, the presentation is similar to dengue fever with body aches, joint pain, fatigue, malaise, fe ...201829779827
genome sequence analysis of a novel bacillus thuringiensis strain blb406 active against aedes aegypti larvae, a novel potential bioinsecticide.blb406 is a novel isolate of bacillus thuringiensis with a larvicidal activity against aedes aegypti larvae. it displays original plasmidic and crystal protein patterns. the present work reported molecular and bioinformatic analyses for the genome sequence of blb406 using miseq illumina next-generation sequencing technology. the reads were assembled by velvet tool. using rast program and pgaap, the genome of blb406 strain was shown to contain 6297 genes corresponding to 5924 protein coding seque ...201829778876
chemical profile and pharmacological effects of the resin and essential oil from bursera slechtendalii: a medicinal "copal tree" of southern mexico.this study reports on the volatile profile and pharmacological activities of the essential oil and resin from bursera slechtendalii, an aromatic medicinal tree commercialized in southern mexico. the essential oil contained 25 volatiles including p-cymen-8-ol (25.4-35.7%), n-hexyl hexanoate (6.8-17.6%) and n-octyl hexanoate (7.6-14.8%), whereas the crystallized resin contained 14 volatiles, including n-octyl acetate (29.6-34.2%), limonene (15.8-18.7%) and n-octyl butyrate (12.6-17-6%). the inhala ...201829777752
modeling dengue vector population using remotely sensed data and machine learning.mosquitoes are vectors of many human diseases. in particular, aedes ægypti (linnaeus) is the main vector for chikungunya, dengue, and zika viruses in latin america and it represents a global threat. public health policies that aim at combating this vector require dependable and timely information, which is usually expensive to obtain with field campaigns. for this reason, several efforts have been done to use remote sensing due to its reduced cost. the present work includes the temporal modeling ...201829777650
use of bead-based serologic assay to evaluate chikungunya virus epidemic, haiti.the index case of chikungunya virus (chikv) in haiti was reported during early 2014; the vector, the pervasive aedes aegypti mosquito, promoted rapid spread throughout the country. during december 2014-february 2015, we collected blood samples from 4,438 persons at 154 sites (62 urban, 92 rural) throughout haiti and measured chikv igg by using a multiplex bead assay. overall chikv seroprevalence was 57.9%; differences between rural (mean 44.9%) and urban (mean 78.4%) areas were pronounced. logis ...201829774861
the effect of nonrandom mating on wolbachia dynamics: implications for population replacement and sterile releases in aedes mosquitoes.wolbachia bacteria are known to cause deviations from random mating and affect sperm competition (sc) in some of their arthropod hosts. because these effects could influence the effectiveness of wolbachia in mosquito population replacement and suppression programs, we developed a theoretical framework to investigate them and we collected relevant data for the wmel infection in aedes aegypti. using incompatibility patterns as a measure of mating success of infected versus uninfected mosquitoes, w ...201829968550
strain-specific pathogenicity and subversion of phenoloxidase activity in the mosquito aedes aegypti by members of the fungal entomopathogenic genus isaria.development of alternative vector control strategies are becoming more pressing given the rapid evolution of insecticide resistance and the rise of vector borne pathogens affecting public health such as dengue, chikungunya and zika. fungal-based biopesticides are promising alternatives to synthetic insecticides because they are ecofriendly and are highly effective at infecting insects through contact. this study evaluated the susceptibility of the yellow fever mosquito ae. aegypti to a range of ...201829967469
continued susceptibility of the wmel wolbachia infection in aedes aegypti to heat stress following field deployment and selection.aedes aegypti mosquitoes infected with the wmel strain of wolbachia are being deployed to control the spread of arboviruses around the world through blockage of viral transmission. blockage by wolbachia in some scenarios may be affected by the susceptibility of wmel to cyclical heat stress during mosquito larval development. we therefore evaluated the potential to generate a heat-resistant strain of wmel in ae. aegypti through artificial laboratory selection and through exposure to field tempera ...201829966368
variation in competence for zikv transmission by aedes aegypti and aedes albopictus in mexico.zikv is a new addition to the arboviruses circulating in the new world, with more than 1 million cases since its introduction in 2015. a growing number of studies have reported vector competence (vc) of aedes mosquitoes from several areas of the world for zikv transmission. some studies have used new world mosquitoes from disparate regions and concluded that these have a variable but relatively low competence for the asian lineage of zikv.201829965958
chikungunya in children: a clinical review.chikungunya (chikv) is an emerging arboviral infection with recent spikes in transmission in the americas. chikungunya is most commonly transmitted by mosquitos, specifically aedes aegypti and aedes albopictus. these mosquitoes are found throughout many parts of the united states. the classic tetrad of symptoms for chikv is fever, symmetric polyarthralgia, maculopapular rash, and nonpurulent conjunctivitis. although the majority (3 of 4) of infected people will be symptomatic, the viral illness ...201829965819
adventitious viruses persistently infect three commonly used mosquito cell lines.mosquito cell lines have been used extensively in research to isolate and propagate arthropod-borne viruses and understand virus-vector interactions. despite their utility as an in vitro tool, these cell lines are poorly defined and may harbor insect-specific viruses. accordingly, we screened four commonly-used mosquito cell lines, c6/36 and u4.4 cells from aedes albopictus, aag2 cells from aedes aegypti, and hsu cells from culex quinquefasciatus, for the presence of adventitious (i.e. exogenous ...201829957338
patterns of occurrence of dengue and chikungunya, and spatial distribution of mosquito vector aedes albopictus in swabi district, pakistan.two principal vector species, aedes aegypti and aedes albopictus, are known for transmission of dengue (den) and chikungunya (chk) in pakistan. we aimed to investigate their spatial and temporal distribution.201829956428
empirical optimization of risk thresholds for dengue: an approach towards entomological management of aedes mosquitoes based on larval indices in the kandy district of sri lanka.larval indices such as premise index (pi), breteau index (bi) and container index (ci) are widely used to interpret the density of dengue vectors in surveillance programmes. these indices may be useful for forecasting disease outbreaks in an area. however, use of the values of these indices as alarm signals is rarely considered in control programmes. therefore, the current study aims to propose threshold values for vector indices based on an empirical modeling approach for the kandy district of ...201829954443
comparison of chikungunya viruses generated using infectious clone or the infectious subgenomic amplicons (isa) method in aedes mosquitoes.reverse genetics systems provide the opportunity to manipulate viral genomes and have been widely used to study rna viruses and to develop new antiviral compounds and vaccine strategies. the recently described method called isa (infectious subgenomic amplicons) gives the possibility to rescue rna viruses in days. we demonstrated in cell culture that the use of the isa method led to a higher genetic diversity of viral populations than that observed using infectious clone technology. however, no r ...201829953474
aedes anphevirus: an insect-specific virus distributed worldwide in aedes aegypti mosquitoes that has complex interplays with wolbachia and dengue virus infection in cells.insect-specific viruses (isvs) of the yellow fever mosquito aedes aegypti have been demonstrated to modulate transmission of arboviruses such as dengue virus (denv) and west nile virus by the mosquito. the diversity and composition of the virome of a. aegypti, however, remains poorly understood. in this study, we characterized aedes anphevirus (aeav), a negative-sense rna virus from the order mononegavirales aeav identified from aedes cell lines was infectious to both a. aegypti and aedes albopi ...201829950416
arthropod evs mediate dengue virus transmission through interaction with a tetraspanin domain containing glycoprotein tsp29fb.dengue virus (denv) is a mosquito-borne flavivirus that causes dengue fever in humans, worldwide. using in vitro cell lines derived from aedes albopictus and aedes aegypti, the primary vectors of denv, we report that denv2/denv3-infected cells secrete extracellular vesicles (evs), including exosomes, containing infectious viral rna and proteins. a full-length denv2 genome, detected in arthropod evs, was infectious to naïve mosquito and mammalian cells, including human-skin keratinocytes and bloo ...201829946031
model-based analysis of experimental data from interconnected, row-configured huts elucidates multifaceted effects of a volatile chemical on aedes aegypti mosquitoes.insecticides used against aedes aegypti and other disease vectors can elicit a multitude of dose-dependent effects on behavioral and bionomic traits. estimating the potential epidemiological impact of a product requires thorough understanding of these effects and their interplay at different dosages. volatile spatial repellent (sr) products come with an additional layer of complexity due to the potential for altered movement of affected mosquitoes and diffusion of volatile particles of the produ ...201829941038
wolbachia enhances insect-specific flavivirus infection in aedes aegypti mosquitoes.mosquitoes transmit a diverse group of human flaviviruses including west nile, dengue, yellow fever, and zika viruses. mosquitoes are also naturally infected with insect-specific flaviviruses (isfs), a subgroup of the family not capable of infecting vertebrates. although isfs are not medically important, they are capable of altering the mosquito's susceptibility to flaviviruses and may alter host fitness. wolbachia is an endosymbiotic bacterium of insects that when present in mosquitoes limits t ...201829938064
using barcoded zika virus to assess virus population structure in vitro and in aedes aegypti mosquitoes.arboviruses such as zika virus (zikv, flaviviridae; flavivirus) must replicate in both mammalian and insect hosts possessing strong immune defenses. accordingly, transmission between and replication within hosts involves genetic bottlenecks, during which viral population size and genetic diversity may be significantly reduced. to help quantify these bottlenecks and their effects, we constructed 4 "barcoded" zikv populations that theoretically contain thousands of barcodes each. after identifying ...201829935423
circulation of chikungunya virus in aedes aegypti in maranhão, northeast brazil.the simultaneous circulation of dengue virus (denv), chikungunya virus (chikv) and zika virus (zikv) arboviruses have placed brazil among the main worldwide endemic areas. brazilian northeast region concentrates the highest incidence of infections caused by chikv and zikv. in maranhão, the second biggest northeastern state, there are cases of human infections caused by these three arboviruses and presence of aedes aegypti and aedes albopictus vectors. in this context, this study aimed to investi ...201829932932
ancient treatment for lice: a source of suggestions for carriers of other infectious diseases?louse infestation is one of the oldest contagious pestilential diseases of humankind, which has recently re-emerged in several developing countries as well as in homeless individuals and migrants. the present work provides the first phase of an historical excursus of louse remedies based on the classics of pharmaceutical literature, codes, pharmacopoeia and treatises. the second phase involves a literature search, based on the principal medical databases (scifinder, pubmed, google scholar, isi-w ...201829932096
evaluation of aedes aegypti (diptera: culicidae) life table attributes upon chikungunya virus replication reveals impact on egg-laying pathways.arthropod-borne virus disease cycles constitute interactions among three primary players-the host, the vector, and the virus-in which the virus needs to interact with the host and the vector to establish its survival. while in the host, some arboviruses replicate aggressively, resulting in host pathogenicity, and manifest as a disease condition. these viruses more often utilize the vectors as reservoirs before they are transmitted to the host and therefore do not amplify to as large titers as th ...201829931258
a high-risk zika and dengue transmission hub: virus detections in mosquitoes at a brazilian university campus.zika virus (zikv) and dengue virus (denv) are mosquito-borne flaviviruses prevalent throughout tropical regions. currently, management of zikv and denv centers on control of the primary vector aedes aegypti. this vector is highly anthropophilic and is therefore prevalent throughout densely urbanised landscapes. a new passive trap for gravid ae. aegypti (gravid aedes trap - gat) was developed for mosquito surveillance. here the different killing agents and the level of transmission of arboviruses ...201829929561
risk factors for the presence of dengue vector mosquitoes, and determinants of their prevalence and larval site selection in dhaka, bangladesh.dengue viruses are responsible for over 100 million infections a year worldwide and are a public health concern in bangladesh. although risk of transmission is high, data on vector population characteristics are scanty in bangladesh; therefore, a comprehensive prediction of the patterns of local virus transmission is not possible. recognizing these gaps, multi-year entomological surveys were carried out in dhaka, where the disease is most frequently reported. the specific objectives of the prese ...201829928055
vector competence of aedes aegypti, culex tarsalis, and culex quinquefasciatus from california for zika virus.zika virus (zikv) has emerged since 2013 as a significant global human health threat following outbreaks in the pacific islands and rapid spread throughout south and central america. severe congenital and neurological sequelae have been linked to zikv infections. assessing the ability of common mosquito species to transmit zikv and characterizing variation in mosquito transmission of different zikv strains is important for estimating regional outbreak potential and for prioritizing local mosquit ...201829927940
hydrophobic nanoprecipitates of β-cyclodextrin/avermectins inclusion compounds reveal insecticide activity against aedes aegypti larvae and low toxicity against fibroblasts.in the present work, hydrophobic nanoprecipitates (hnps) of inclusion complexes formed between β-cyclodextrin (βcd) and the avermectins (avms) named eprinomectin (epri) and ivermectin (iver) were synthesized and characterized, and their larvicidal activity against aedes aegypti and human safety against fibroblasts were evaluated. initially, thermogravimetric analysis/differential thermal analysis data revealed that inclusion increased the thermal stability of avms in the presence of βcd. nuclear ...201829925239
field testing & evaluation of the efficacy & duration of effectiveness of a biolarvicide, bactivec® sc (bacillus thuringiensis var. israelensis sh-14) in bengaluru, india.different formulations of bacillus thuringiensis var. israelensis (bti) have been tested against different mosquito vectors and other insects for their residual activity. in the present study, the efficacy and residual activity of a new formulation of bti (bactivec suspension concentrate) were evaluated against immature stages of anopheles stephensi liston (diptera: culicidae), aedes aegypti linnaeus (diptera: culicidae) and culex quinquefasciatus say (diptera: culicidae), in natural habitats in ...201829923520
salinity tolerant aedes aegypti and ae. albopictus-infection with dengue virus and contribution to dengue transmission in a coastal peninsula.aedes aegypti and ae. albopictus are major arboviral vectors that are considered to lay eggs, and undergo preimaginal development only in fresh water collections. however, recently they have been also shown to develop in coastal brackish water habitats. the ability of the biologically variant salinity-tolerant aedes vectors to transmit arboviral diseases is unknown. we therefore, investigated the infection of salinity-tolerant aedes mosquitoes with dengue virus (denv) and analysed dengue inciden ...201829916445
screening of natural wolbachia infection in aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus from guadeloupe (french west indies).guadeloupe islands are threatened by several mosquito-borne viruses such as dengue, chikungunya, zika and west nile virus. it appears essential to look for alternative mosquito control methods such as the incompatible insect technique (itt) aiming at sterilizing wild females by inundative releases of incompatible males. before considering the implementation of such a strategy, the characterization of genetic diversity of the endocellular bacterium wolbachia regarding the local mosquito populatio ...201829908171
prediction and prevention of urban arbovirus epidemics: a challenge for the global virology community.the recent emergence and rapid spread of zika virus in tropical regions of the western hemisphere took arbovirologists and public health officials by surprise, and the earlier transfers of west nile and chikungunya viruses from the old to the new world were also unexpected. these pandemics underscore the increasing threat of zoonotic arboviruses, especially those that are capable of entering into human-amplified, urban transmission cycles transmitted by aedes (stegomyia) aegypti and sometimes ot ...201829906475
lack of evidence for natural wolbachia infections in aedes aegypti (diptera: culicidae).wolbachia is a genus of endosymbiotic bacteria that infects 66% of all insect species. its major impact on insects is in reproduction: sterility, production of one sex, and/or parthenogenesis. another effect was discovered when the disease-transmitting mosquito, aedes aegypti linnaeus (diptera: culicidae), was infected with wolbachia isolated from drosophila: infected female mosquitoes became less capable of transmitting diseases such as dengue fever and chikungunya. this has led to releases of ...201829901734
restoration of pyrethroid susceptibility in a highly resistant aedes aegypti population.insecticide resistance has evolved in disease vectors worldwide, creating the urgent need to either develop new control methods or restore insecticide susceptibility to regain use of existing tools. here we show that phenotypic susceptibility can be restored in a highly resistant field-derived strain of aedes aegypti in only 10 generations through rearing them in the absence of insecticide.201829899128
signifying zika: heterogeneity in the representations of the virus by history of infection.despite having been broadly advertised by the mass media, many negative consequences of the zika virus have been less significant than originally predicted. it is likely that after a few months from the epidemic's onset, personal experience with the virus has altered the person's way to deal with the disease. this study explores the relation between exposure to zika virus and the social representation of the epidemic. more specifically, one analyzes if increased exposure to the risk of zika infe ...201829898004
evaluation of the effects of the larvicides temephos on reproductive performance, embryofetal development and dna integrity of swiss mice.temephos is considered the gold standard by the ministry of health for controlling the larvae of the mosquito aedes aegypti. the present study evaluated the effects of temephos larvicide on the reproductive performance, embryo-fetal development and dna integrity of swiss mice. this study used 30 pregnant female mice: 10 were controls treated with drinking water at a dosage of 0.1 ml/10 g (body weight - b.w., administered orally - a.o.), and 20 were treated with temephos at doses of 0.0043 mg/kg ...201829891373
what does the future hold for yellow fever virus? (i).the recent resurgence of yellow fever virus (yfv) activity in the tropical regions of africa and south america has sparked renewed interest in this infamous arboviral disease. yellow fever virus had been a human plague for centuries prior to the identification of its urban transmission vector, the aedes (stegomyia) aegypti (linnaeus) mosquito species, and the development of an efficient live-attenuated vaccine, the yf-17d strain. the combination of vector-control measures and vaccination campaig ...201829890711
a transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, aedes aegypti.populations of aedes aegypti naturally exhibit variable susceptibility to dengue viruses. this natural variation can be impacted by nutritional stress resulting from larval-stage crowding, indicating the influence of environment components on the adult mosquito immune response. in particular, larval crowding was previously shown to reduce the susceptibility of adult females of a trinidad field isolate of a. aegypti to the dengue serotype 2 (jam1409) virus. here, we present the first whole transc ...201829889847
target and non-target toxicity of fern extracts against mosquito vectors and beneficial aquatic organisms.dengue and malaria are significant mosquito-borne diseases that are rapidly spread worldwide, mainly in temperate countries. pteridophytes were identified to be a significant source of novel mosquitocidal agents. the present research was to explore the eco-friendly larvicides from methanol extracts of ferns, viz., actiniopteris radiata, adiantum caudatum, cheilanthes swartzii, hemionitis arifolia and lycopodium clavatum. the larvicidal potential of the extracts screened using larvae of dengue ve ...201829885618
viral determinants and vector competence of zika virus transmission.zika virus (zikv) has emerged as a new global health threat. since its first discovery in zika forest in uganda, this virus has been isolated from several mosquito species, including aedes aegypti and aedes albopictus. the geographical distribution of these mosquito species across tropical and subtropical regions has led to several outbreaks, including the recent pandemic in brazil, followed by the pacific islands and other areas of north and south america. this has gained attention of the scien ...201829875751
multiscale approach to deciphering the molecular mechanisms involved in the direct and intergenerational effect of ibuprofen on mosquito aedes aegypti.the anti-inflammatory ibuprofen is a ubiquitous surface water contaminant. however, the chronic impact of this pharmaceutical on aquatic invertebrate populations remains poorly understood. in model insect aedes aegypti, we investigated the intergenerational consequences of parental chronic exposure to an environmentally relevant concentration of ibuprofen. while exposed individuals did not show any phenotypic changes, their progeny showed accelerated development and an increased tolerance to sta ...201829874051
effects of temephos, permethrin, and eucalyptus nitens essential oil on survival and swimming behavior of aedes aegypti and anopheles pseudopunctipennis (diptera: culicidae) larvae.an essential strategy to deal with mosquito-borne diseases is the control of larvae in their development sites. the mosquitoes anopheles pseudopunctipennis (theobald) (diptera: culicidae), a malaria vector, and aedes aegypti (l.) (diptera: culicidae), vector of dengue, zika, yellow fever, and chikungunya viruses, breed in very different habitats. insecticide treatments of mosquito larvae focus mainly on their lethal effects. however, insecticide degradation or the poor dosage of larvicides will ...201829873741
vertical transmission of dengue virus in the yogyakarta airport area.international health regulations controls international travel including human movement, disease vector, and imported items to prevent the spread of dengue, especially in seaports, airports, and border crossing posts. this study aimed to determine dengue transovarial transmission index (tti) and distribution of dengue virus in the areas around adisucipto airport of yogyakarta, indonesia.201829871615
differential effects of temperature and mosquito genetics determine transmissibility of arboviruses by aedes aegypti in argentina.aedes aegypti (l.) (diptera: culicidae) have a global distribution and are the primary vector of a number of mosquito-borne viruses responsible for epidemics throughout the americas. as in much of south america, the threat from pathogens including dengue virus (denv; flaviviridae, flavivirus) and chikungunya virus (chikv; togaviridae, alphavirus) has increased in argentina in recent years. the complexity of transmission cycles makes predicting the occurrence and intensity of arbovirus outbreaks ...201829869610
serosurvey of human antibodies recognizing aedes aegypti d7 salivary proteins in colombia.dengue is one of the most geographically significant mosquito-borne viral diseases transmitted by aedes mosquitoes. during blood feeding, mosquitoes deposit salivary proteins that induce antibody responses. these can be related to the intensity of exposure to bites. some mosquito salivary proteins, such as d7 proteins, are known as potent allergens. the antibody response to d7 proteins can be used as a marker to evaluate the risk of exposure and disease transmission and provide critical informat ...201829868532
effect of the topical repellent para-menthane-3,8-diol on blood feeding behavior and fecundity of the dengue virus vector aedes aegypti.dengue fever is an acute disease caused by the dengue virus and transmitted primarily by the mosquito aedes aegypti. the current strategy for dengue prevention is vector control including the use of topical repellents to reduce mosquito biting. although n,n-diethyl-m-methylbenzamide (deet) is the most common active ingredient in topical repellent products, para-menthane-3,8-diol (pmd) is also used commercially. studies have indicated pmd reduced biting by 90⁻95% for up to 6⁻8 h, similar to the e ...201829867036
pyrethroid resistance in the dengue vector aedes aegypti in southeast asia: present situation and prospects for management.human arboviral diseases transmitted by aedes aegypti such as dengue, zika, chikungunya and yellow fever remain global public health threats to date. of these diseases, dengue fever is particularly prevalent in southeast asia. relentless vector control efforts are performed to curtail disease transmissions through which pyrethroid insecticides are broadly used as the first line of defense to control ae. aegypti, especially in the course of disease outbreaks. here, we compile the largest contempo ...201829866193
molecular and functional characterization of single-box high-mobility group b (hmgb) chromosomal protein from aedes aegypti.high-mobility group b (hmgb) proteins have highly conserved, unique dna-binding domains, hmg boxes, that can bind non-b-type dna structures, such as bent, kinked and unwound structures, with high affinity. hmgb proteins also promote dna bending, looping and unwinding. in this study, we determined the role of the aedes aegypti single hmg-box domain protein aahmgb; characterized its structure, spatiotemporal expression levels, subcellular localization, and nucleic acid binding activities; and comp ...201829859286
technologies to combat aedes mosquitoes: a model based on smart city.aedes aegypti and aedes albopictus mosquitoes are responsible for the transmission of diseases such as dengue fever, yellow fever, chikungunya fever, zika virus fever, some of which can cause irreversible central nervous system problems and death. this study investigates what technologies are being used for combatting and monitoring the aedes mosquitoes and to propose joining these technologies into a single and complete solution using the smart cities concept. a search for newscasts on google a ...201829857404
exposure of a diurnal mosquito vector to floral mimics: foraging responses, feeding patterns, and significance for sugar bait technology.food location by mosquitoes is mediated by resource-derived olfactory and visual signals. smell sensation is intermittent and dependent on the environment, whereas visual signals are continual and precede olfactory cues. success of mosquito bait technology, where olfactory cues are used for attraction, is being impeded by reduced attractiveness. despite proof that mosquitoes respond to colored objects, including those mimicking floral shape, and that they can discriminate among flowers, the impa ...201829856985
the awed trial (applying wolbachia to eliminate dengue) to assess the efficacy of wolbachia-infected mosquito deployments to reduce dengue incidence in yogyakarta, indonesia: study protocol for a cluster randomised controlled trial.dengue and other arboviruses transmitted by aedes aegypti mosquitoes, including zika and chikungunya, present an increasing public health challenge in tropical regions. current vector control strategies have failed to curb disease transmission, but continue to be employed despite the absence of robust evidence for their effectiveness or optimal implementation. the world mosquito program has developed a novel approach to arbovirus control using ae. aegypti stably transfected with wolbachia bacter ...201829855331
a highly secure method for rearing aedes aegypti mosquitoes.vector-borne infectious diseases are caused by pathogenic microorganisms transmitted mainly by blood-sucking arthropod vectors. in laboratories, the handling of insects carrying human pathogens requires extra caution because of safety concerns over their escape risk. based on standard insect containment practices, there have been cases where costly enhancements were required to definitely protect laboratory workers and neighbors from potential infection through mosquito bites. here, we developed ...201829849477
dengue in singapore from 2004 to 2016: cyclical epidemic patterns dominated by serotypes 1 and 2.singapore has experienced periodic dengue epidemics despite maintaining a low aedes house index. each epidemic was associated with a switch in the predominant serotype. we investigated the temporal dynamics of dengue fever and dengue virus (denv) and analyzed the epidemiological and entomological patterns of dengue in singapore from 2004 to 2016. the case surveillance is based on a mandatory notification system that requires all medical practitioners to report clinically suspected and laboratory ...201829848407
report of chikungunya virus in wild populations of aedes aegypti in guerrero state, mexico.we detected vertical transmission of chikungunya virus (chikv) in wild populations of aedes aegypti from san marcos, guerrero, mexico, with real-time reverse transcriptase-polymerase chain reaction. a total of 20 pools (1-11 specimens/pool) of larvae, male, and female mosquitoes were tested. we report the detection of chikv in 2 of 11 larval pools, 4 of 5 male pools, and 1 of 4 female pools, from field-collected mosquitoes.201831442150
a survey of the mosquito species in maxcanu, yucatan, mexico.a survey was carried out to identify the mosquitoes inhabiting human premises in the rural locality of maxcanú, yucatán, mexico. using the centers for disease control and prevention backpack aspirators, simple random sampling was carried out inside of 101 homes during the november 2013 rainy season. a total of 1,492 specimens were collected. three subfamilies (anophelinae, culicinae, and toxorhynchitinae) and 5 species were identified: anopheles albimanus, aedes aegypti, culex interrogator, lima ...201831442149
a survey of the mosquitoes of kosrae state, federated states of micronesia, 2016.in response to an outbreak of zika virus that started in february 2016 on kosrae island, kosrae state, federated states of micronesia, we conducted entomological investigations, including a survey to characterize the mosquito fauna on kosrae, from november 29 to december 8, 2016. mosquitoes were collected using several surveillance methods in order to sample all stages of the mosquito life cycle. eggs were collected using ovicups, larvae and pupae were sampled using standard dippers, and adults ...201831414080
[knowledge and practices in aedes aegypti control among different social subjects in salvador, bahia state, brazil].due to the persistence of dengue and other arbovirus infections in brazil, the government has stepped up measures to combat the aedes aegypti mosquito vector. the responsibilities of community endemic disease workers (cedw) and community health workers (chw) include acting as intermediaries and disseminating knowledge in the community. the aim of this study was to analyze knowledge and practices in dengue control by different social subjects: residents and cedw/chw. interviews were held with res ...201829846399
follow up of a robust meta-signature to identify zika virus infection in aedes aegypti: another brick in the wall.the mosquito aedes aegypti is the main vector of several arthropod-borne diseases that have global impacts. in a previous meta-analysis, our group identified a vector gene set containing 110 genes strongly associated with infections of dengue, west nile and yellow fever viruses. of these 110 genes, four genes allowed a highly accurate classification of infected status. more recently, a new study of ae. aegypti infected with zika virus (zikv) was published, providing new data to investigate wheth ...201829846381
the use of wolbachia by the world mosquito program to interrupt transmission of aedes aegypti transmitted viruses.the biological control of mosquito transmission by the use of the naturally occurring insect-specific bacterial endosymbiont wolbachia has been successfully tested in small field trials. the approach has been translated successfully to larger field sites in townsville, australia and expanded to more than 10 countries through the eliminate dengue program. the broader application of the program beyond limiting the transmission of dengue and including other aedes aegypti borne mosquitoes has seen t ...201829845544
structures of zika virus e & ns1: relations with virus infection and host immune responses.zika virus (zikv), first discovered in the zika forest in uganda in 1947 was understudied until the recent explosive epidemic in several south american countries where it has become strongly associated with congenital birth defects leading to severe cranial malformations and neurological conditions. the increase in number of case of microcephaly in newborn children associated with zikv infection triggered the world health organization to declare the epidemic as a public health emergency of inter ...201829845526
back-to-the-future potential for autochthonous transmission of aedes aegypti-transmitted viruses in ethekwini and urban coastal kwazulu-natal province, south africa. 201829843848
the risk of urban yellow fever resurgence in aedes-infested american cities.aedes aegypti, historically known as yellow fever (yf) mosquito, transmits a great number of other viruses such as dengue, west nile, chikungunya, zika, mayaro and perhaps oropouche, among others. well established in africa and asia, aedes mosquitoes are now increasingly invading large parts of the american continent, and hence the risk of urban yf resurgence in the american cities should because of great concern to public health authorities. although no new urban cycle of yf was reported in the ...201829843824
evaluation of larvicidal, adulticidal, and anticholinesterase activities of essential oils of illicium verum hook. f., pimenta dioica (l.) merr., and myristica fragrans houtt. against zika virus vectors.aedes aegypti is the vector responsible for transmitting pathogens that cause various infectious diseases, such as dengue, zika, yellow fever, and chikungunya, worrying health authorities in the tropics. due to resistance of mosquitoes to synthetic insecticides, the search for more effective insecticidal agents becomes crucial. the aim of this study was to verify the larvicidal, adulticidal, and anticholinesterase activities of the essential oils of the illicium verum (eoiv), pimenta dioica (eop ...201829808407
rapid, noninvasive detection of zika virus in aedes aegypti mosquitoes by near-infrared spectroscopy.the accelerating global spread of arboviruses, such as zika virus (zikv), highlights the need for more proactive mosquito surveillance. however, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. we show for the first time that near-infrared spectroscopy (nirs) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect zikv in heads and thoraces of intact aedes aegypti mosquitoes with ...201829806030
impact of short-term temperature challenges on the larvicidal activities of the entomopathogenic watermold leptolegnia chapmanii against aedes aegypti, and development on infected dead larvae.the oomycete leptolegnia chapmanii is among the most promising entomopathogens for biological control of aedes aegypti. this mosquito vector breeds in small water collections, where this aquatic watermold pathogen can face short-term scenarios of challenging high or low temperatures during changing ambient conditions, but it is yet not well understood how extreme temperatures might affect the virulence and recycling capacities of this pathogen. we tested the effect of short-term exposure of ency ...201829801786
vapor toxicity of five volatile pyrethroids against aedes aegypti, aedes albopictus, culex quinquefasciatus, and anopheles quadrimaculatus (diptera: culicidae).mosquito mortality has been documented in numerous studies of spatial repellents but the concentration-dependent toxicity of spatial repellent vapors has not been documented. to address this issue, prallethrin, flumethrin, metofluthrin, transfluthrin, and meperfluthrin were selected for comparative study against aedes albopictus (skuse), ae. aegypti (l.), culex quinquefasciatus say, and anopheles quadrimaculatus say. mosquito were exposed to vapors of each chemical for 2, 4, and 24 h with mortal ...201829797795
codon adaptation biases among sylvatic and urban genotypes of dengue virus type 2.dengue virus (denv) emerged from the sylvatic environment and colonized urban settings, being sustained in a human-aedes-human transmission chain, mainly by the bites of females of the anthropophilic species aedes aegypti. herein, we sought evidence for fine-tuning in viral codon usage, possibly due to viral adaptation to human transmission. we compared the codon adaptation of denv serotype 2 (denv-2) genotypes from urban and sylvatic habitats and tried to correlate the findings with key evoluti ...201829792991
surveillance of mosquitoes (diptera: culicidae) in southern iowa, 2016.the mosquito fauna of iowa has been extensively investigated over several decades, providing a wealth of information regarding species distributions, relative abundance, temporal activity patterns, and identifying vectors of medical importance. however, these investigations have had unequal coverage, leaving the mosquito fauna in some parts of the state, including southern iowa, largely uncharacterized. with the heightened public health threat of zika virus in the summer of 2016, greater emphasi ...201829788488
threshold dynamics of a temperature-dependent stage-structured mosquito population model with nested delays.mosquito-borne diseases remain a significant threat to public health and economics. since mosquitoes are quite sensitive to temperature, global warming may not only worsen the disease transmission case in current endemic areas but also facilitate mosquito population together with pathogens to establish in new regions. therefore, understanding mosquito population dynamics under the impact of temperature is considerably important for making disease control policies. in this paper, we develop a sta ...201829785519
a multi-faceted pandemic: a review of the state of knowledge on the zika virus.while until recently the small and isolated zika outbreaks in eastern asia and pacific islands had been overlooked, the large-scale outbreak that started in brazil in 2015 and the increase of microcephaly cases in the same place and time made media headlines. considered as harmless until recently, zika has given rise to an important global crisis that poses not only health challenges but also environmental, economical, social, and ethical challenges for states and people around the world. the ma ...201829785319
the influence of urban heat islands and socioeconomic factors on the spatial distribution of aedes aegypti larval habitats.we addressed the potential associations among the temporal and spatial distribution of larval habitats of aedes (stegomyia) aegypti, the presence of urban heat islands and socioeconomic factors. data on larval habitats were collected in santa bárbara d'oeste, são paulo, brazil, from 2004 to 2006, and spatial and temporal variations were analysed using a wavelet-based approach. we quantified urban heat islands by calculating surface temperatures using the results of wavelet analyses and grey leve ...201829772891
urban environmental clustering to assess the spatial dynamics of aedes aegypti breeding sites.the identification of aedes aegypti breeding hotspots in urban areas is crucial for the rational design of control strategies against this disease vector. remote sensing and geographic information systems offer valuable tools for mapping habitat suitability of a given area. however, predicting species occurrences by means of probability distribution maps based on transversal entomological surveys has limited utility for local authorities. the aim of the present study was to carefully examine the ...201829772886
encapsidated host factors in alphavirus particles influence midgut infection of aedes aegypti.transmission of mosquito-borne viruses requires the efficient infection of both a permissive vertebrate host and a competent mosquito vector. the infectivity of sindbis virus (sinv), the type species of the alphavirus genus, is influenced by both the original and new host cell. we have shown that infection of vertebrate cells by sinv, chikungunya virus (chikv), and ross river virus (rrv) produces two subpopulations of virus particles separable based on density. in contrast, a single population o ...201829772674
tracking aedes aegypti (diptera: culicidae) larval behavior across development: effects of temperature and nutrients on individuals' foraging behavior.immature mosquitoes alter their foraging behavior in response to variation in nutrients, predators, and temperature, with consequences on the adult stage where pathogens are transmitted. these patterns of behavior have not been described with respect to both developmental stage and environmental variation, nor has behavior been examined within an individual across instars. we hypothesized that individual larvae have distinct behavioral syndromes, and predict that the rank of foraging activity in ...201829771372
niche conservatism of aedes albopictus and aedes aegypti - two mosquito species with different invasion histories.biological invasions have been associated with niche changes; however, their occurrence is still debated. we assess whether climatic niches between native and non-native ranges have changed during the invasion process using two globally spread mosquitoes as model species, aedes albopictus and aedes aegypti. considering the different time spans since their invasions (>300 vs. 30-40 years), niche changes were expected to be more likely for ae. aegypti than for ae. albopictus. we used temperature a ...201829769652
ornamental bromeliads of miami-dade county, florida are important breeding sites for aedes aegypti (diptera: culicidae).a major public health concern is the emergence and geographical spread of vector-borne diseases such as zika and yellow fever. ornamental bromeliads retaining water in their leaf axils represent potential breeding sites for mosquitoes. as the role of ornamental bromeliads in breeding aedes aegypti in miami-dade county, florida is unknown, we hypothesize that ornamental bromeliads are important breeding sites for ae. aegypti. our objective was to survey bromeliads in areas with high densities of ...201829769105
efficacy of the main repellents available in the brazilian market against aedes aegypti bites under concentrations applied to pediatric populations. 201829768569
ns1 codon usage adaptation to humans in pandemic zika virus.zika virus (zikv) was recognised as a zoonotic pathogen in africa and southeastern asia. human infections were infrequently reported until 2007, when the first known epidemic occurred in micronesia. after 2013, the asian lineage of zikv spread along the pacific islands and americas, causing severe outbreaks with millions of human infections. the recent human infections of zikv were also associated with severe complications, such as an increase in cases of guillain-barre syndrome and the emergenc ...201829768530
pyrethroid insecticides maintain repellent effect on knock-down resistant populations of aedes aegypti mosquitoes.pyrethroid-treated clothing is commonly worn for protection against mosquitoes; pyrethroids are both insecticides and repellents. pyrethroid resistance has become increasingly common in aedes aegypti, the vector of dengue, zika, and other arboviruses, but it is not clear whether resistance is associated with reductions in repellency. in order to determine whether long-lasting permethrin impregnated (llpi) clothing is protective, we used aedes aegypti from new orleans, la (pyrethroid-sensitive) a ...201829763445
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