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resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in wild accessions of gossypium hirsutum and g. barbadense from mexico.forty-six accessions of g. hirsutum and two of g. barbadense were examined for resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in environmental growth chamber experiments, with the objective of finding new sources of resistance. only the g. barbadense accessions, tx-1347 and tx-1348, supported significantly less reproduction by r. reniformis than the susceptible control, deltapine 16 (usda accession sa-1186). however, they were highly susceptible to m. incognita race 3. t ...199719274280
suppression mechanisms of meloidogyne arenaria race 1 by pasteuria penetrans.the biological control of meloidogyne arenaria on peanut (arachis hypogaea) by pasteuria penetrans was evaluated using a six x six factorial experiment in field microplots over 2 years. the main factors were six inoculum levels of second-stage juveniles (j2) of m. arenaria race 1 (0, 40, 200, 1,000, 5,000, and 25,000 j2/microplot, except that the highest level was 20,000 j2/microplot in 1995) and six infestation levels of p. penetrans as percentages of j2 with endospores attached (0, 20, 40, 60, ...199719274127
replacement series: a tool for characterizing competition between phytoparasitic nematodes.the replacement series approach was used to detect and define competition between meloidogyne incognita (mi) and rotylenchulus reniformis (rr) on soybean. in three greenhouse tests, soybean cv. davis seedlings were inoculated with 1,000 vermiform nematodes in the following mi:rr ratios: 0:0, 100:0, 75:25, 50:50, 25:75, and 0:100. after 86 days, relative nematode-yield values (number of each species in mixed culture divided by number in nonmixed culture) were calculated based on nematodes in soil ...199719274132
meloidogyne trifoliophila n. sp. (nemata: meloidogynidae), a parasite of clover from tennessee.meloidogyne trifoliophila n. sp. is described from white clover collected at ames plantation, fayette county, tennessee. the perineal pattern is rounded, with long, smooth striae and rounded arch, and without distinct lateral lines or perivulval striae. the female stylet is 12.6-15.5 mum long, the excretory pore is level with or up to one stylet length posterior to the stylet knobs, and the vulva is subterminal. the posterior terminus is weakly protuberant. the male lateral field is composed of ...199719274133
host-parasite relationships of meloidogyne trifoliophila isolates from new zealand.root-infecting nematodes are commonly found on white clover in new zealand pasture where they reduce yield, nitrogen fixation, and persistence. the dominant root-knot nematode on white clover in new zealand is confirmed in this study as meloidogyne trifoliophila by isozyme phenotype comparison with the type population from tennessee. results from a host differential test differed in the host ranges of m. trifoliophila and m. hapla from new zealand locations, with m. trifoliophila failing to repr ...199719274134
interaction of fusarium oxysporum f. sp. ciceri and meloidogyne javanica on cicer arietinum.interaction of meloidogyne javanica and fusarium oxysporum f. sp. ciceri was studied on fusarium wilt-susceptible (jg 62 and k 850) and resistant (jg 74 and avrodhi) chickpea cultivars. in greenhouse experiments, inoculation of m. javanica juveniles prior to f. oxysporum f. sp. ciceri caused greater wilt incidence in susceptible cultivars and induced vascular discoloration in roots of resistant cultivars. nematode reproduction was greatest (p = 0.05) at 25 degrees c. number of galls and percenta ...199719274140
extraction of root-associated meloidogyne incognita and rotylenchulus reniformis.a technique based on physical maceration of root tissue was developed to extract vermiform and swollen stages of meloidogyne incognita and rotylenchulus reniformis. experiments conducted on soybean and tomato evaluated the efficiency of method (stir, grind), naoc1 concentration (0%, 0.5%), and duration (lx, 2x) on extraction of nematodes and eggs from 60-day-old populations. root-associated populations of r. reniformis were considerably lower than those of m. incognita, so development of the met ...199719274151
temperature-dependent development of pasteuria penetrans in meloidogyne arenaria.pasteuria penetrans is a promising biological control agent of plant-parasitic nematodes. this study was conducted to determine effects of temperature on the bacterium's development in meloidogyne arenaria. developmental stages of p. penetrans were viewed with a compound microscope and verified with scanning electron microscopy within each nematode at 100 accumulated degree-day intervals by tracking accumulated degree-days at three temperatures (21, 28, and 35 degrees c). five predominant develo ...199719274154
antibodies from chicken eggs as probes for antigens from pasteuria penetrans endospores.the bacteria pasteuria spp. have been identified as among the most promising of several microbial organisms currently under investigation as biological control agents of plant-parasitic nematodes. as part of our goal to develop methods to discriminate isolates of pasteuria penetrans with different host preferences, we investigated the potential of developing antibody probes to identify endospores of different isolates of p. penetrans. polyclonal igy antibodies were raised in chickens against end ...199719274158
estimating incidence of attachment of pasteuria penetrans endospores to meloidogyne spp. with tally thresholds.pasteuria penetrans has .been identified as an important biological control agent of root-knot nematodes. in this study the use of tally thresholds was evaluated for estimating p. penetrans endospore attachment to second-stage juveniles (j2) of meloidogyne spp. a tally threshold (t) is defined as the maximum number of individuals in a sample unit that may be treated as absent based on binomial sampling. three different data sets that originated from centrifugal bioassay, incubation bioassay, and ...199719274161
tolerance to rotylenchulus reniformis and resistance to meloidogyne incognita race 3 in high-yielding breeding lines of upland cotton.field experiments in 1992 and 1994 were conducted to determine the effect of rotylenchulus reniformis, reniform nematode, on lint yield and fiber quality of 10 experimental breeding lines of cotton (gossypium hirsutum) in untreated plots or plots fumigated with 1,3-dichloropropene. controls were la. rn 1032, a germplasm line possessing some resistance to r. reniformis, and stoneville 453, a cultivar that is susceptible to reniform nematode. several breeding lines produced greater lint yields tha ...199719274165
differential response to root-knot nematodes in prunus species and correlative genetic implications.responses of 17 prunus rootstocks or accessions (11 from the subgenus amygdalus and 6 from the subgenus prunophora) were evaluated against 11 isolates of meloidogyne spp. including one m. arenaria, four m. incognita, four m. javanica, one m. hispanica, and an unclassified population from florida. characterization of plant response to root-knot nematodes was based on a gall index rating. numbers of females and juveniles plus eggs in the roots were determined for 10 of the rootstocks evaluated aga ...199719274170
relationship between meloidogyne incognita and rotylenchulus reniformis as influenced by soybean genotype.the effect of soybean genotype on competition between meloidogyne incognita race 2 (mi) and rotylenchulus reniformis (rr) was evaluated in greenhouse and microplot replacement series experiments. soil in pots containing seedlings of 'davis' (susceptible to mi) or 'buckshot 66' (resistant to mi) was infested with 1,000 vermiform individuals in the following mi:rr ratios: 0:0, 100:0, 75:25, 50:50, 25:75, or 0:100. after 91 days, the relative nematode yields (number of nematodes in mixed culture di ...199719274173
temperature effects on the attachment of pasteuria penetrans endospores to meloidogyne arenaria race 1.pasteuria penetrans is a gram positive bacterium that prevents meloidogyne spp. from reproducing and diminishes their ability to penetrate roots. the attachment of the endospores to the cuticle of the nematodes is the first step in the life cycle of the bacterium and is essential for its reproduction. as a preliminary study to a field solarization test, the effects of temperature on the attachment of p. penetrans on meloidogyne arenaria race 1 were investigated. preexposing second-stage juvenile ...199719274192
effects of monoclonal antibodies, cationized ferritin, and other organic molecules on adhesion of pasteuria penetrans endospores to meloidogyne incognita.the incidence of adhesion of pasteuria penetrans endospores to meloidogyne incognita second-stage juveniles (j2) was studied after pretreatment of the latter with monoclonal antibodies (mab), cationized ferritin, and other organic molecules in replicated trials. monoclonal antibodies developed to a cuticular epitope of m. incognita second-stage juveniles gave significant reductions in attachment of p. penetrans endospores to treated nematodes. mab bound to the entire length of j2 except for the ...199719274193
root-knot nematode management in dryland taro with tropical cover crops.twenty-two cover crops were evaluated for their ability to reduce damage by root-knot nematode, meloidogyne javanica, to taro, colocastia esculenta, in a tropical cropping system. cover crops were grown and incorporated into the soil before taro was planted. barley, greenpanic, glycine, marigold, sesame, sunn hemp, and sorghum x sudangrass dekalb st6e were poor or nonhosts to the nematode as measured by low population changes of nematodes in soil between cover crop planting and taro planting. al ...199719274275
curative and residual efficacy of injection applications of avermectins for control of plant-parasitic nematodes on banana.studies were conducted to determine the curative and residual efficacy of avermectins at controlling plant-parasitic nematodes when injected into the pseudostem of banana, musa acuminata cv. cavendish. in addition, we determined the lowest concentration of avermectins that provided satisfactory efficacy as protectants when injected into banana pseudostems. experiments were conducted with a root-knot nematode, meleidogyne javanica, and the burrowing nematode, radopholus similis. injections (1 ml) ...199719274271
the first isolated collagen gene of the root-knot nematode meloidogyne javanica is developmentally regulated.the nematode's surface comprises a multilayered cuticle, which consists mainly of collagen proteins. we identified, cloned and characterized the first cuticular collagen gene, mjcol-3, of the plant-parasitic nematode meloidogyne javanica. the gene putatively encodes a 32.4-kda collagen protein, including a propeptide which possesses a subtilisin-like protease-cleavage site. six introns were identified in the gene sequence, with three slightly different acceptor-splicing sites. the basic structur ...19979322758
genetic variation in nacobbus aberrans: an approach toward taxonomic resolution.biochemical and molecular analyses of genetic variation were evaluated to address the taxonomic status of nacobbus aberrans. isolates from mexico, peru, and argentina, cultured on tomato in the greenhouse, were analyzed with respect to isozyme and dna marker variation. although acid phosphatase and malate dehydrogenase revealed distinct profiles for each isolate, non-specific esterases revealed possible affinities between the peruvian isolates and between the isolates from mexico and peru. two o ...199719274155
regulated use of an alternative spliced leader exon in the plant parasitic nematode meloidogyne javanica. 19979178273
effect of gamma-irradiation and heat on root-knot nematode, meloidogyne javanica.effects of gamma-irradiation on the root-knot nematode meloidogyne javanica were investigated. a dose of 7.5 kgy killed all second-stage juveniles (j2) within 1 day after treatment. egg hatch was completely inhibited at 6.25 kgy. a bioassay on tomato measuring galling and egg production was used to determine the infectivity of irradiated j2 and j2 hatched from irradiated eggs. the j2 and eggs irradiated with a dose of 4.25 kgy did not induce galls or reproduce on tomato plants. when nematodes we ...199719274131
impact of meloidogyne incognita on the incidence of peach tree short life in the presence of criconemella xenoplax.the relationship between cricenemella xenoplax alone and in combination with meloidogyne incognitaon the incidence of peach tree short life disease was studied in field microplots during 1989-96. the presence of m. incognita suppressed the population density of c. xenoplax on lovell peach. tree trunk diameter was significantly reduced in the presence of both nematode species prior to 1993. soil ph was lowest in the co-infection treatment as compared with the uninoculated control on three of the ...199719274276
colored mulches affect yield of fresh-market tomato infected with meloidogyne incognita.the effects of different-colored polyethylene mulches on the quantity and spectra of reflected light, earliness of fruit set, fruit yield and quality, and root-knot disease were studied in field-grown, staked tomato (lycopersicon esculentum). white mulch reflected more photosynthetic light and a lower far-red-to-red ratio than red mulch, whereas black mulch reflected less than 5 percent of any color. soil temperatures and fruit yields were recorded for tomato plants inoculated with meloidogyne i ...199719274191
evaluation of nemx, a new cultivar of cotton with high resistance to meloidogyne incognita.the level of resistance to root-knot nematode, meloidogyne incognita, in nemx, a new cultivar of the acala-type upland cotton, was evaluated in relation to four resistant breeding lines (n6072, n8577, n901, and n903) and four susceptible cultivars (maxxa, sj2, royale, and prema). in growth pouch tests, an average of only 4 nematode egg masses was produced on roots of nemx or the resistant lines, compared to a significantly higher average of 21 on the susceptible cultivars. in pot tests, the nema ...199719274190
activity and differential induction of chitinase isozymes in soybean cultivars resistant or susceptible to root-knot nematodes.host physiological events in relation to infestation by parasitic nematodes are not well documented. soybean plant responses to meloidogyne incognita infestation were compared to resistant (bryan) and susceptible (brim) cultivars at 0, 1, 3, 10, 20, and 34 days after infestation (dai). the resistant cultivar had higher chitinase activity than the susceptible cultivar at every sample time beginning at 3 dai. results from isoelectric focusing gel electrophoresis analyses indicated that three acidi ...199719274189
meloidogyne incognita inoculum source affects host suitability and growth of yellow nutsedge and chile pepper.meloidogyne incognita (mi) reproduction and host plant responses in chile pepper (capsicum annuum) and yellow nutsedge (cyperus esculentus = yns) to three sources of inoculum obtained by rearing a single mi population on chile, yns, and tomato were evaluated in two factorial greenhouse experiments. the interactive effects of mi inoculum source and crop-weed competition were determined. in the absence of yns competition, chile growth was reduced less by mi inoculum from chile than by inoculum fro ...199719274174
suppression of meloidogyne arenaria race 1 by soil application of endospores of pasteuria penetrans.the potential of pasteuria penetrans for suppressing meloidogyne arenaria race 1 on peanut (arachis hypogaea) was tested over a 2-year period in a field microplot experiment. endospores of p. penetrans were mass-produced on m. arenaria race 1 infecting tomato plants. endospores were inoculated in the first year only at rates of 0, 1,000, 3,000, 10,000, and 100,000 endospores/g of soil, respectively, into the top 20 cm of microplots that were previously infested with m. arenaria race 1. one peanu ...199619277131
meloidogyne paranaensis n. sp. (nemata: meloidogynidae), a root-knot nematode parasitizing coffee in brazil.a root-knot nematode parasitizing coffee in paran state, brazil, is described as meloidogyne paranaensis n. sp. the suggested common name is paraná coffee root-knot nematode. the perineal pattern is similar to that of m. incognita; the labial disc and medial lips of the female are fused and asymmetric and rectangular; the lateral lips are small, triangular, and fused laterally with the head region. the female stylet is 15.0-17.5 mum long, with broad, distinctly set-off knobs; the distance from t ...199619277133
attachment of pasteuria penetrans endospores to the surface of meloidogyne javanica second-stage juveniles.pasteuria penetrans spore adhesion to meloidogyne javanica second-stage juveniles (j2) was examined following several different pretreatments of the latter. the detergents sodium dodecyl sulfate and triton x-100, the carbohydrates fucose and alpha-methyl-d-mannoside, and the lectins concanavalin a and wheat germ agglutinin reduced spore attachment. spores exposed to m. javanica surface coat (sc) extract exhibited decreased adherence to the j2 surface. second-stage juveniles that had been treated ...199619277150
meloidogyne incognita infested soil amended with chicken litter.the effects of chicken litter on meloidogyne incognita in cotton, gossypium hirsutum cv. dpl50 were determined in field microplots. litters (manure and pine-shaving bedding) from a research facility and a commercial broiler house were used. treatments consisted of 0.25%, 0.5%, and 1% litter by dry weight of soil for each kind of litter. three control treatments consisted of soil not amended with litter, with and without nematodes, and one treatment to which mineral fertilizer was added at a nitr ...199619277155
glucuronidase expression in transgenic tobacco roots with a parasponia promoter on infection with meloidogyne javanica.the expression of a g-us reporter gene linked to a parasponia andersonii hemoglobin promoter has been studied in transgenic tobacco plants after infection by meloidogyne javanica. transgenic roots were harvested at different times after nematode inoculation, and stained histochemically for expression of the gus gene. during the early stages of infection (0-2 weeks) there was little expression in giant cells, in contrast to other cells of the root. in later stages of infection (3-6 weeks) there w ...199619277159
impact of soil texture on the reproductive and damage potentials of rotylenchulus reniformis and meloidogyne incognita on cotton.the effects of soil type and initial inoculum density (pi) on the reproductive and damage potentials of meloidogyne incognita and rotylenchulus reniformis on cotton were evaluated in microplot experiments from 1991 to 1993. the equilibrium nematode population density for r. reniformis on cotton was much greater than that of m. incognita, indicating that cotton is a better host for r. reniformis than m. incognita. reproduction of m. incognita was greater in coarse-textured soils than in fine-text ...199619277171
greenhouse evaluation of selected soybean germplasm for resistance to north carolina populations of heterodera glycines, rotylenchulus reniformis, and meloidogyne species.selected soybean genotypes were evaluated for resistance to north carolina populations of the soybean cyst nematode heterodera glycines, the root-knot nematodes meloidogyne incognita races 3 and 4, m. arenaria races 1 and 2, m. javanica, and the reniform nematode rotylenchulus reniformis in two greenhouse tests. populations of cyst nematode used in the first test were cultures from field samples originally classified as races 1-5, and those used in the second test included inbred cyst lines that ...199619277179
prunus rootstock evaluation to root-knot and lesion nematodes in spain.two screening and one resistance verification trial involving 20 prunus rootstocks were conducted under greenhouse conditions against meloidogyne spp. and pratylenchus vulnus. most of the rootstocks were experimental genotypes or new commercial peach and plums of spanish and french origin. nearly all are interspecific hybrid rootstocks. in the first trial, the rootstocks bruce, cadaman, mirac, g x n no. 15, cachirulo x (g x n no. 9), and p. myra x peach were immune or resistant to a mixture of s ...199619277184
damage potential and reproduction of meloidogyne incognita race 3 and m. arenaria race 1 on kenaf.the effects of meloidogyne incognita race 3 and m. arenaria race 1 on growth of kenaf cv. everglades 41 was determined under greenhouse conditions. seedlings of kenaf were inoculated with initial population densities (pi) of 0, 625, 1,250, 2,500, 5,000, and 10,000 eggs/plant and placed on greenhouse benches in a randomized complete block design. plant growth and nematode reproduction were assessed 6 and 12 weeks after inoculation. growth suppression of kenaf in response to increasing pi was obse ...199619277193
suppression of meloidogyne incognita and m. javanica by pasteuria penetrans in field soil.the role of pasteuria penetrans in suppressing numbers of root-knot nematodes was investigated in a 7-year monocuhure of tobacco in a field naturally infested with a mixed population of meloidogyne incognita race 1 and m. javanica. the suppressiveness of the soil was tested using four treatments: autoclaving (ac), microwaving (mw), air drying (dr), and untreated. the treated soil bioassays consisted of tobacco cv. northrup king 326 (resistant to m. incognita but susceptible to m. javanica) and c ...199619277344
quantification of endospore concentrations of pasteuria penetrans in tomato root material.six methods for quantification of the endospore concentrations of pasteuria penetrans from tomato roots are described. mortar disruption and machine disruption methods gave the highest estimations (endospores per gram of root material) of 83.7 and 79.0 million, respectively. these methods were significantly superior to incubation bioassay (47.7 million), enzymatic disruption (32.1 million), and enzymatic disruption + flotation (25.8 million) methods. a centrifugation bioassay method gave the low ...199619277345
additive effects of meloidogyne arenaria and sclerotinin rolfsii on peanut.field observations have suggested that infection of peanut by meloidogyne arenaria increases the incidence of southern blight caused by sclerotium rolfsii. three factorial experiments in microplots were conducted to determine if interactions between m. arenaria and s. rolfsii influenced final nematode population densities, incidence of southern blight, or pod yield. treatments included four or five initial population densities of m. arenaria and three inoculum rates of s. rolfsii. final nematode ...199619277351
expression and functional characterization of a single chain fv antibody directed against secretions involved in plant nematode infection process.expression in plants of antibodies directed against proteins essential for pathogenesis could provide an alternative approach to engineer new resistance traits into crops. salivary secretions of the root-knot nematode meloidogyne incognita are known to play a key role during this nematode infection process. from a hybridoma cell line producing an igm monoclonal antibody specific to these secretions, we have constructed a synthetic gene that encodes an antigen-binding single-chain fv protein (scf ...19968645292
gold-conjugated reagents for the labeling of carbohydrate-recognition domains and glycoconjugates on nematode surfaces.various fluorescent conjugated lectins have been used for the detection of glycoconjugates on nematode surfaces under light microscopy. several problems have been experienced with these reagents including penetration of the cuticle by fluorescent lectins, non-glycoconjugate specificity, strong nematode autofluorescence at the emission wavelength of the fluorescent dye, and prevention of persistent visualization due to rapid quenching of the fluorescent components. gold-conjugated reagents combin ...199619277354
image analysis of the growth of globodera pallida and meloidogyne incognita on transgenic tomato roots expressing cystatins.an approach based on image analysis that enables rapid collection and analysis of nematode size and shape during growth is reported. this technique has been applied to assess meloidogyne incognita and globodera pallida during their development over 35 and 42 days, respectively, on transgenic tomato roots expressing the wild-type rice cystatin oc-i or an engineered variant, oc-iad86. morphometric values were established that subdivided enlarged saccate females from other life stages. analysis of ...199619277136
responses of meloidogyne arenaria and m. incognita to green manures and supplemental urea in glasshouse culture.the recent loss of many effective nematicides has led to renewed interest in alternative methods of nematode management. greenhouse experiments were conducted to determine the effects of rapeseed and velvetbean green manures, and supplemental urea, on the root-knot nematodes meloidogyne arenaria and m. incognita. green manures were incorporated with m. arenaria-infested soil using rates totaling 200,300, and 400 mg n/kg soil. squash plants grown in this soil were evaluated using a gall index and ...199619277190
identification of rapd, scar, and rflp markers tightly linked to nematode resistance genes introgressed from arachis cardenasii into arachis hypogaea.two dominant genes conditioning resistance to the root-knot nematode meloidogyne arenaria were identified in a segregating f2 population derived from the cross of 4x (arachis hypogaea x arachis cardenasii)-ga 6 and pi 261942. mae is proposed as the designation for the dominant gene restricting egg number and mag is proposed as the designation for the dominant gene restricting galling. the high levels of resistance in ga 6 were introgressed from a. cardenasii and, therefore, a search to identify ...19968890516
role of nematodes, nematicides, and crop rotation on the productivity and quality of potato, sweet potato, peanut, and grain sorghum.the objective of this experiment was to determine the effects of fenamiphos 15g and short-cycle potato (po)-sweet potato (sp) grown continuously and in rotation with peanut (pe)-grain sorghum (gs) on yield, crop quality, and mixed nematode population densities of meloidogyne arenaria, m. hapla, m. incognita, and mesocriconema ornatum. greater root-gall indices and damage by m. hapla and m. incognita occurred on potato than other crops. most crop yields were higher and root-gall indices lower fro ...199619277157
penetration and post-infectional development and reproduction of meloidogyne arenaria races 1 and 2 on susceptible and resistant soybean genotypes.penetration, post-infectional development, reproduction, and fecundity of meloidogyne arenaria races 1 and 2 were studied on susceptible (cns), partially resistant (jackson), and highly resistant (pi 200538 and pi 230977) soybean genotypes in the greenhouse. the ability to locate and invade roots was similar between races, but more juveniles penetrated roots of susceptible cns than the resistant genotypes. at 10 days after inoculation, 56% and 99% to 100% of race 1 second-stage juveniles were ve ...199619277152
cellular responses of resistant and susceptible soybean genotypes infected with meloidogyne arenaria races 1 and 2.the cellular responses induced by meloidogyne arenaria races 1 and 2 in three soybean genotypes, susceptible cns, resistant jackson, and resistant pi 200538, were examined by light microscopy 20 days after inoculation. differences in giant-cell development were greater between races than among the soybean genotypes. m. arenaria race 1 stimulated small, poorly formed giant-cells in contrast with m. arenaria race 2, which induced well-developed, thick-walled, multinucleate giant-cells. the number ...199619277138
surface coat of meloidogyne incognita.the nematode surface coat is defined as an extracuticular component on the outermost layer of the nematode body wall, visualized only by electron microscopy. surface coat proteins of meloidogyne incognita race 3 infective juveniles were characterized by electrophoresis and western blotting of extracts from radioiodine and biotin-labeled nematodes. extraction of labeled nematodes with cetyltrimethylammonium bromide yielded a principal protein band larger than 250 kda and, with water soluble bioti ...199619277137
inheritance of resistance to the root-knot nematode meloidogyne arenaria in myrobalan plum.the inheritance of resistance of the self-incompatible myrobalan plum prunus cerasifera to the root-knot nematode meloidogyne arenaria was studied using first a diallel cross between five parents of variable host suitability (including two highly resistant clones p.1079 and p.2175, a moderate host p.2032, a good host p.2646 and an excellent host p.16.5), followed by the g2 crosses p.16.5 × (p.2646 × p.1079) and p.2646 × (p.16.5 × p.1079). a total of 355 g1 and 72 g2 clones obtained from hard-woo ...199624166553
phloem mobility of xenobiotics viii. a short review.great strides have been made in the last 15 years in our understanding of phloem mobility of xenobiotics. the subject has been transformed from a poorly understood phenomenon to a process that can be accurately described by the physicochemical properties of the xenobiotic and the nature of the vascular system through which it moves. the basic tenet of the unified mathematical model is that the combination of the permeability and the acid dissociation constant (pk(a)) determines phloem mobility, ...199621245258
host suitability of twelve leguminosae species to populations of meloidogyne hapla and m. chitwoodi.legumes of the genera astragalus (milkvetch), coronilla (crownvetch), lathyrus (pea vine), lotus (birdsfoot trefoil), medicago (alfalfa), melilotus (clover), trifolium (clover), and vicia (common vetch) were inoculated with a population of melaidogyne chitwoodi from utah or with one of three m. hapla populations from california, utah, and wyoming.thirty-nine percent to 86% of alfalfa (m. scutellata) and 10% to 55% of red clover (t. pratense) plants survived inoculation with the nematode populati ...199619277158
host suitability of 32 common weeds to meloidogyne hapla in organic soils of southwestern quebec.thirty-two weeds commonly found in the organic soils of southwestern quebec were evaluated for host suitability to a local isolate of the northern root-knot nematode meloidogyne hapla under greenhouse conditions. galls were observed on the roots of 21 species. sixteen of the 21 had a reproduction factor (pf/pi = final number of m. hapla eggs and juveniles per initial number of m. hapla juveniles per pot) higher than carrot (pf/pi = 0.37), the major host crop in this agricultural area. tomato cv. ...199619277189
response of additional herbaceous perennial ornamentals to meloidogyne hapla.twenty-nine herbaceous perennial ornamentals were evaluated for root galling after 2 months in soil infested with meloidogyne hapla u n d e r greenhouse conditions. plants such as asclepias, epimedium, liriope, lithospermura, myosotis, penstemon, sidalecea, and solidago did not have galls or egg masses present on the root system and were rated as resistant. astrantia, boltonia, centranthus, and miscanthus had more than 100 galls on the roots (similar to 'rutgers' tomato controls) and were rated ...199619277187
mentha x piperita, mentha spicata and effects of their essential oils on meloidogyne in soil.six peppermint (mentha x piperita) and six spearmint (m. spicata) pi accessions were inoculated with meloidogyne incognita race 3 and m. arenaria race 2, under greenhouse conditions. no galls formed on roots of any of the plants inoculated with 1,800 eggs/pot. fewer than two galls per root system formed on three pi accessions of peppermint inoculated with m. incognita at 5,400 eggs/pot. only one peppermint accession developed galls when inoculated with m. arenaria, whereas none of the spearmint ...199619277186
early root response to meloidogyne incognita in resistant and susceptible alfalfa cultivars.the early events of meloidogyne incognita behavior and associated host responses following root penetration were studied in resistant (cv. moapa 69) and susceptible (cv. lahontan) alfalfa. ten-day-old seedlings of alfalfa cultivars were inoculated with second-stage juveniles (j2) and harvested 12, 24, 48, and 72 hours and 7, 14, and 21 days later. both cultivars supported similar root penetration and initial j2 migration. by 72 hours after inoculation the majority of j2 were amassed inside the v ...199619277165
differentiation of meloidogyne incognita and m. arenaria novel resistance phenotypes in lycopersicon peruvianum and derived bridge-lines.lycopersicon peruvianum pi 270435 clone 2r2 and pi 126443 clone 1mh were crossed reciprocally with three l. esculentum-l. peruvianum bridge-lines. the incongruity barrier between the two plant species was overcome; f1 progeny were obtained from crosses between four parental combinations without embryo-rescue culture. hybridity was confirmed by leaf and flower morphology and by the production of nematode-resistant f1 progeny on homozygous susceptible parents. clones of the five f1 bridgeline hybr ...199624162431
effect of simulated rainfall on efficacy and leaching of two formulations of fenamiphos.recoverable fenamiphos in the soil and residue in squash following different simulated rainfall treatments after nematicide application were determined in a 2-year study. efficacy of fenamiphos also was evaluated. fenamiphos treatments (3 sc and 15 g) were broadcast (6.7 kg a.i./ha) over plots and incorporated into the top 15 cm of soil immediately before planting 'dixie hybrid' squash. simulated rainfall treatments of 0, 2.5, and 5.0 cm water were applied 1 day after fenamiphos application. soi ...199619277156
comparison of two sympatric pasteuria populations isolated from a tropical vertisol soil.an isolate of pasteuria (designated ppmj) recovered from the root-knot nematode meloidogyne javanica, was characterized using host preference, spore morphometrics, and serology, and compared with another sympatric pasteuria isolate (designated pphc) collected from the cyst nematode, heterodera cajani. ppmj spores were larger (x 1.5) than the pphc spores and had a mean diameter of 3.4 μm after fixation for electron microscopy. the central body of ppmj spores was about twice as big as the central ...199624415311
repulsion of meloidogyne incognita by alginate pellets containing hyphae of monacrosporium cionopagum, m. ellipsosporum, or hirsutella rhossiliensis.the responses of second-stage juveniles (j2) of meloidogyne incognita race 3 to calcium alginate pellets containing hyphae of the nematophagous fungi monacrosporiura cionopagum, m. ellipsosporum, and hirsutella rhossiliensis were examined using cylinders (38-mm-diam., 40 or 72 mm long) of sand (94% <250-mum particle size). sand was wetted with a synthetic soil solution (10% moisture, 0.06 bar water potential). a layer of 10 or 20 pellets was placed 4 or 20 mm from one end of the cylinder. after ...199619277129
a molecular study of root-knot nematode-induced feeding sites.in a compatible interaction, root-knot nematodes (meloidogyne) induce a sophisticated feeding site shortly after they have penetrated the plant root. the feeding site contains metabolically highly active giant cells. to gain insight into the molecular aspects that are typical for giant cells, a cdna library from tomato roots infected with meloidogyne incognita was differentially screened to find induced genes. among the genes identified, two extensin genes (lemmi8 and lemmi11) and a lea-like gen ...19968580972
meloidogyne partityla on pecan isozyme phenotypes and other host.meloidogyne sp. from five pecan (carya illinoensis) orchards in texas were distinctive in host range and iszoyme profiles from common species of meloidogyne but were morphologically congruent with meloidogyne partityla kleynhans, a species previously known only in south africa. in addition to pecan, species of walnut (juglans hindsii and j. regia) and hickory (c. ovata) also were hosts. no reproduction was observed on 15 other plant species from nine families, including several common hosts of o ...199619277175
effects of resistance in phaseolus vulgaris on development of meloidogyne species.use of resistant phaseolus vulgaris germplasm has a potential role in limiting damaging effects of meloidogyne spp. on bean production. effects of two genetic resistance systems in common bean germptasm on penetration and development of meloidogyne spp. were studied under growth room conditions at 22 degrees c to 25 degrees c. nemasnap (gene system 1) and g1805 (gene system 2) were inoculated with second-stage juveniles (j2) of m. incognita race 2 and m. arenaria race 1, respectively; black vale ...199619277166
induction of cdc2a and cyc1at expression in arabidopsis thaliana during early phases of nematode-induced feeding cell formation.root-knot and cyst nematodes are plant parasites that induce large multinucleated feeding cells in the roots of their hosts. cytological observations have shown that root-knot nematodes induce giant cells by cycles of mitosis without cytokinesis whereas cyst nematodes provoke cell wall degradation leading to the formation of a large syncytium. this study was intended to characterize and compare the ability of both types of nematodes to induce progression through the cell cycle. for this purpose, ...19969011085
identification of resistance to meloidogyne javanica in the lycopersicon peruvianum complex.clones of lycopersicon peruvianum pi 2704352r2, pi 270435-3mh and pi 126443-1mh expressed novel resistance to three mi-avirulent m. javanica isolates in greenhouse experiments. clones from pi 126443-1mh were resistant to the three m. javanica isolates at 25°c. the three isolates were able to reproduce on one embryorescue hybrid of pi 126443-1mh, but not on three l. peruvianum-l. esculentum bridge-line hybrids of pi 1264431mh when screened at 25°c (mi-expressed temperature). clones of pi 270435-2 ...199624162423
application of mitochondrial dna polymorphism to meloidogyne molecular population biology.recent advances in molecular biology have enabled the genotyping of individual nematodes, facilitating the analysis of genetic variability within and among plant-pathogenic nematode isolates. this review first describes representative examples of how rflp, rapd, aflp, and dna sequence analysis have been employed to describe populations of several phytonematodes, including the pinewood, burrowing, root-knot, and cyst nematodes. the second portion of this paper evaluates the utility of a size-vari ...199619277144
rflp analysis of resistance to columbia root-knot nematode derived from solanum bulbocastanum in a bc2 population.the mapping of resistance tomeloidogyne chitwoodi derived from solarium bulbocastanum is reported. a population suitable for mapping was developed as follows. a somatic hybrid of nematode-resistant s. bulbocastanum and cultivated tetraploid potato was produced. this was backcrossed to tetraploid potato, and a single resistant bc1 was selected and backcrossed again to the same recurrent tetraploid parent. the mapping population consisted of 64 bc2 progeny scored for restriction fragment length po ...199624166325
response of perennial herbaceous ornamentals to meloidogyne hapla.sixty-nine herbaceous perennial ornamentals in 56 genera were evaluated for root galling after 2 months in soil infested with meloidogyne hapla under greenhouse conditions. plants were rated susceptible or resistant based on the number of galls present on the root system. thirty-six percent had more than 100 galls on the roots (similar to 'rutgers' tomato controls) and were rated susceptible. thirty percent of the plants tested did not have galls or egg masses present on the root system and were ...199519277335
induced resistance to meloidogyne hapla by other meloidogyne species on tomato and pyrethrum plants.advance inoculation of the tomato cv. celebrity or the pyrethrum clone 223 with host-incompatible meloidogyne incognita or m. javanica elicited induced resistance to host-compatible m. hapla in pot and field experiments. induced resistance increased with the length of the time between inoculations and with the population density of the induction inoculum. optimum interval before challenge inoculation, or population density of inoculum for inducing resistance, was 10 days, or 5,000 infective nema ...199519277310
satellite dna as a target for pcr-specific detection of the plant-parasitic nematode meloidogyne hapla.the polymerase chain reaction was evaluated for its ability to amplify dna sequences specific for the root-knot nematode meloidogyne hapla, using oligonucleotides whose sequence was deduced from the satellite dna previously cloned in this species as primers. as expected, ladder patterns of monomers and multimers of an approximate 150-170-bp repeat were amplified from purified genomic dna of all the m. hapla isolates studied, while no amplification was detected with the five other meloidogyne spe ...19958593688
characterization of resistance in a somatic hybrid of solanum bulbocastanum and s. tuberosum, to meloidogyne chitwoodi.a somatic hybrid, cbp-233, between resistant solanum bulbocastanum (sb-22) and susceptible s. tuberosum (r4) was tested for resistance to meloidogyne chitwoodi race 1. one week after inoculation, only 0.04-0.4% of the initial inoculum (pi, 5,000 eggs) as second stage-juveniles infected sb-22 and cbp-233 root systems, compared to 2% in r4. after 8 weeks, the number of m. chitwoodi in sb-22 and cbp-233 roots remained lower (0.3-1.5% of pi) compared to r4, which increased from 2% to ca. 27%. develo ...199519277265
reproduction of meloidogyne arenaria, m. incognita, and m. javanica on sesame.reproduction of meloidogyne arenaria race 1, m. ineognita races 1 and 3, and m. javanica on 10 cultivars of sesame (sesame indicum) was examined in greenhouse tests. sesame cultivars were also evaluated in a field infested with m. arenaria. sesame was a poor host for m. incognita races 1 and 3 as no sesame genotype supported more than 70 eggs/g root. reproduction of m. arenaria race 1 on sesame varied from 20 eggs/g roots for cultivar sesaco 7cb to 1,570 eggs/g roots for sesaco 119 in the greenh ...199519277331
effects of peanut genotypes on meloidogyne species interactions.a 3-year microplot study was conducted to characterize the interaction between meloidogyne arenaria race 1 (ma1) and m. hapla (mh), as affected by the five peanut genotypes: florigiant, nc 7, nc 6, nc ac 18416, and nc ac 18016. the interactive effects on infection (total parasitic forms per root unit) and reproduction potentials of each nematode species and crop damage were determined. as a single population, ma1 had greater infection capacity and caused more crop damage than did mh, but both sp ...199519277279
intra- and interpopulation genome variation in meloidogyne arenaria.the genetic heterogeneity of two m. arenaria race 2 populations (designated pelion and govan) was examined using rflp analysis of 12 clonal lines established from single egg masses (six distinct clonal lines from each population). these populations are essentially identical by traditional biochemical and race identification schemes; however, the govan population is more aggressive than the pelion population, producing larger galls and exhibiting greater reproductive capabilities on many soybean ...199519277274
characterization of a pathogen-induced potato catalase and its systemic expression upon nematode and bacterial infection.we have isolated a cdna encoding a catalase (cat2st) by differential screening of a cdna library constructed from potato roots infected with the cyst nematode globodera pallida. expression analysis confirmed the local induction of cat2st and showed that it was highest at the adult stage of the parasite. it also revealed that cat2st was induced in uninfected roots, stems, and leaves of infected plants. localized and systemic induction of cat2st was also observed upon root-knot nematode (meloidogy ...19957655060
a basic serine protease from paecilomyces lilacinus with biological activity against meloidogyne hapla eggs.scanning electron micrographs of the nematode-egg-parasitic fungus paecilomyces lilacinus infecting eggs of the root-knot nematode meloidogyne spp. suggested the involvement of lytic enzymes. when grown on a liquid mineral salts medium, supplemented with different substrates as the sole n- and c-source, the fungus produced an extracellular protease. colloidal chitin, vitellin and intact eggs of the root-knot nematode meloidogyne hapla induced proteolytic activity that was repressed by glucose. t ...19957773385
carbohydrate-recognition domains on the surface of phytophagous nematodes.human red blood cells (hrbc) adhered to preparasitic second-stage juveniles (j2) of heterodera avenae, heterodera schachtii, meloidogyne javanica, pratylenchus mediterraneus, rotylenchulus reniformis, and tylenchulus semipenetrans over the entire nematode body. binding was conspicuously confined to the head and tail of longidorus cohni, xiphinema brevicolle, and xiphinema index. binding was ca2+ and mg2+ dependent. in contrast, hrbc did not adhere to anguina tritici, aphelenchoides subtenius, di ...19957895833
embryogenesis and postinfection development of meloidogyne konaensis.the effects of temperature on embryogenesis and postinfection development in meloidogyne konaensis were examined. embryogenesis was evaluated at 5, 8, 10, 13, 16, 20, 22, 24, 26, 28, 30, 35, and 40 c. no embryonic development occurred at 5 c. some development, although incomplete, occurred at 8 and 10 c. the rate of embryogenesis was linear from 13 to 30 c, but decreased at 35 c. the lowest egg mortality occurred at 24 c, whereas all eggs died within 24 hours at 40 c. postinfection development w ...199519277267
spatial-temporal patterns of meloidogyne konaensis on coffee in hawaii.population densities ofmeloidogyne konaensis were determined in march and july of 1991 and 1992 on coffee cultivars guatemalan and 502, and on four rootstocks (purpuree, congensis, deweveri, and kaffe) with guatemalan or 502 as a scion. three-dimensional spatial patterns were characterized on roots of guatemalan and deweveri. population densities differed among rootstocks (p < 0.05) and times (p < 0.01). the greatest number of second-stage juveniles (j2) occurred on guatemalan and fewest j2 on p ...199519277268
the surface coat of plant-parasitic nematodes: chemical composition, origin, and biological role-a review.chemical composition, origin, and biological role of the surface coat (sc) of plant-parasitic nematodes are described and compared with those of animal-parasitic and free-living nematodes. the sc of the plant-parasitic nematodes is 5-30 nm thick and is characterized by a net negative charge. it consists, at least in part, of glycoproteins and proteins with various molecular weights, depending upon the nematode species. the lability of its components and the binding of human red blood cells to th ...199519277272
infection, reproduction potential, and root galling by root-knot nematode species and concomitant populations on peanut and tobacco.single populations of meloidogyne arenaria races 1 (ma1) and 2 (ma2) and m. hapla (mh), and mixed populations of ma1 + ma2 and ma1 + mh with four inoculum levels of eggs were tested on peanut cv. 'florigiant' and m. incognita-resistant tobacco cv. 'mcnair 373' in a greenhouse experiment. root infection, female development, and reproduction of ma2 on peanut and ma1 on resistant tobacco were limited at 2 and 6 weeks. ma1, mh, and ma1 + mh on peanut had similar root infection (total parasitic forms ...199519277277
atomic force microscopy of plant-parasitic nematodes.a simple method for atomic force microscopy (afm) of nematode cuticle was developed to visualize the external topography of helicotylenchus lobus, meloidogyne javanica, m. incognita, and xiphinema diversicaudatum. endospores of two isolates of the nematode parasite, pasteuria penetrans, adhering to m. incognita and x. diversicaudatum were also visualized and measured by this technique. scanning procedures were applied to specimens killed and dehydrated in air or dehydrated and stored in glycerol ...199519277280
temporal efficacy of selected nematicides on meloidogyne species on tobacco.aldicarb, ethoprop, and fenamiphos were evaluated for their efficacy in controlling various species of root-knot nematodes on flue-cured tobacco and for their residual activity, as determined through periodic sampling and bioassays of soil taken from field plots. field experiments were conducted at five locations over 2 years with flue-cured tobacco. soil in plots treated with nematicides were formed into high, wide beds before transplanting with 'coker 371-gold' or 'k 326' tobacco. residual con ...199519277289
enzymes of the phenylpropanoid pathway in soybean infected with meloidogyne incognita or heterodera glycines.transcription of genes encoding several enzymes and the activity of some of these enzymes of the phenylpropanoid pathway leading to synthesis of chemical and physical barriers for defense of plants against root pathogens was estimated in susceptible and resistant soybean infected with heterodera glycines race 3 or with meloidogyne incognita race 3. transcription of genes encoding phenylalanine ammonia lyase (pal) and the activity of this enzyme increased in resistant, but not susceptible, soybea ...199519277292
occurrence of pasteuria-like organisms on selected plant-pamsitic nematodes of pineapple in the hawaiian islands.soils from 320 sites representing diverse undisturbed habitats from five hawaiian islands were assessed for occurrence of pasteuria-like organisms. mean annual rainfall at sites ranged from 125-350 cm, elevation from 69-2,286 m, and annual mean temperature from 12-24 c. seven different natural communities were represented: wet lowland, mesic lowland, wet montane, mesk montane, dry montane, mesic subalpine, and dry alpine. pasteuria spp. in a soil sample was detected by baiting with infective sta ...199519277305
rotations with coastal bermudagrass and fallow for management of meloidogyne incognita and soilborne fungi on vegetable crops.the efficacy of fallow and coastal bermudagrass (cynodon dactylon) as a rotation crop for control of root-knot nematode (meloidogyne incognita race 1) and soilborne fungi in okra (hibiscus esculentus cv. emerald), squash (cucurbita pepo cv. dixie hybrid), and sweet corn (zea mays cv. merit) was evaluated in a 3-year field trial. numbers of m. incognita in the soil and root-gall indices were greater on okra and squash than sweet corn and declined over the years on vegetable crops following fallow ...199519277312
responses of some common cruciferae to root-knot nematodes.ten cultivated plants of the family cruciferae were evaluated for susceptibility to meloidogyne arenaria race 1, m. incognita races 1 and 3, and m. javanica in a series of four separate greenhouse tests. after 62-64 days, or 1,032-1,072 degree days (10 c base), several of the crops evaluated showed moderate to severe levels of galling (> 3.0 on 0-5 scale) and moderate numbers of egg masses (>2.0 on 0-5 scale) in response to each of the nematode species and races. among the plants tested, collard ...199519277321
mixtures of olive pomace with different nitrogen sources for the control of meloidogyne spp. on tomato.the efficacy of mixtures of dry olive (olea europea) pomace with biuret, guanidine, and melamine for control of root-knot nematodes (meloidogyne spp.) on tomato (lycopersicon esculentum) was studied in greenhouse experiments. olive pomace (op) applied pre-plant at 10 g/kg soil was phytotoxic. mixtures of op (10 g/kg soil) with biuret or guanidine at 200-300 mg/kg soil reduced or eliminated the phytotoxic effect, controlled root-knot nematodes, and increased soil esterase activity indicative of m ...199519277325
comparison of crop rotation and fallow for management of heterodera glycines and meloidogyne spp. in soybean.the effects of cropping systems (fallow, rotation with sorghum-sudangrass hybrid [sorghum bicolor x s. sudanense], and continuous soybean [glycine max]), nematicide (aldicarb) treatment, and soybean cultivar on yield and nematode population densities were studied in a field infested with a mixture of meloidogyne spp. and heterodera glycines. soybean following sorghum-sudangrass yielded 111 kg/ha more than soybean following fallow and 600 kg/ha more than continuous soybean. aldicarb treatment inc ...199519277326
effect of cutting age on the resistance of prunus cerasifera (myrobalan plum) to meloidogyne arenaria.the response of softwood cuttings of myrobalan plum infested after 50 and 105 days with 3,000 second-stage juveniles (j2) of meloidogyne arenaria was compared to 15-month-old hardwood cuttings in 13 genotypes ranging from highly resistant to susceptible. gall index and number of galls were recorded 30 days after infestation. fifty-day-old cuttings rooted in perlite developed many rootlets, but had only incipient galls after infestation. in sand, rooting of 50-day-old cuttings not treated with in ...199519277333
co-infection of wilt-resistant chickpeas by fusarium oxysporum f. sp. ciceri and meloidogyne javanica.fusarium oxysporum f. sp. ciceri and meloidogyne javanica are important pathogens of chickpea. interrelationships between fusarium oxysporum f. sp. ciceri and m. javanica were investigated on 53 fusarium wilt-resistant chickpea genotypes in pot experiments. all of the genotypes were susceptible to m. javanica. fusarium wilt resistance in one genotype (icc 12275) was ineffective in the presence of m. javanica, and all the plants completely wilted. resistance in four genotypes (iccs 11319, 11322, ...199519277336
mapping a new nematode resistance locus in lycopersicon peruvianum.accessions of the wild tomato species l. peruvianum were screened with a root-knot nematode population (557r) which infects tomato plants carrying the nematode resistance gene mi. several accessions were found to carry resistance to 557r. a l. peruvianum backcross population segregating for resistance to 557r was produced. the segregation ratio of resistant to susceptible plants suggested that a single, dominant gene was a major factor in the new resistance. this gene, which we have designated m ...199524169835
effects of irrigation, nitrogen, and a nematicide on pearl millet.pearl millet is used mainly as a temporary forage crop in the southern united states. a new pearl millet hybrid has potential as a major grain crop in the united states. the effects of nematodes, irrigation, a nematicide, and nitrogen rates on a new pearl millet grain hybrid, hgm-100, and nematode population changes were determined in a 2-year study. root-knot nematodes (meloidogyne incognita race 1) entered the roots of pearl millet and caused minimal galling, but produced large numbers of eggs ...199519277324
effects and carry-over benefits of nematicides in soil planted to a sweet corn-squash-vetch cropping system.the effects of irrigation on the efficacy of nematicides on meloidogyne incognita race 1 population densities, yield of sweet corn, and the carry-over of nematicidal effect in the squash crop were determined in a sweet corn-squash-vetch cropping system for 3 years. fenamiphos 15g and aldicarb 15g were applied at 6.7 kg a.i./ha and incorporated 15 cm deep with a tractor-mounted rototiller. ethylene dibromide (edb) was injected at 18 kg a.i./ha on each side of the sweet corn rows (total 36 kg a.i. ...199519277323
effect of simulated rainfall on leaching and efficacy of fenamiphos.there is increasing concern in the united states about the pesticide movement in soil, groundwater contamination, and pesticide residue in food. the objective of this study was to determine the efficacy, degradation, and movement of fenamiphos (nemacur 15g) in the soil and residues in squash fruit as influenced by four simulated rainfall treatments (2.5 or 5.0 cm each applied 1 or 3 days after nematicide application) under field conditions. in 1990, concentrations of fenamiphos were greater in t ...199519277322
influence of meloidogyne incognita on the water relations of cotton grown in microplots.the effects of meloidogyne incognita on the growth and water relations of cotton were evaluated in a 2-year field study. microplots containing methyl bromide-fumigated fine sandy loam soil were infested with the nematode and planted to cotton (gossypium hirsutum l.). treatments included addition of nematodes alone, addition of nematodes plus the insecticide-nematicide aldicarb (1.7 kg/ha), and an untreated control. meloidogyne incognita population densities reached high levels in both treatments ...199519277313
effect of ammonium ions on egg hatching and second-stage juveniles of meloidogyne incognita in axenic tomato root culture.eggs, either dispersed or in masses, and second-stage juveniles (j2) of meloidogyne incognita were exposed to different concentrations of ammonium ions in a nutrient agar medium upon which excised tomato roots were growing. egg hatch and j2 penetration of the roots was slowed or inhibited at high (54 and 324 mg/liter) but not at low (1.5 and 9 mg/liter) concentrations of ammonium nitrate. the effect of ammonium on j2 appeared to be temporary and reversible. high potassium nitrate concentration ( ...199519277298
root cortical cell spherical bodies associated with an induced resistance reaction in monoxenic cultures of meloidogyne incognita.the root-knot nematode meloidogyne incognita was monoxenically cultured on excised roots of soybean cv. pickett and tomato cv. rutgers in agar media containing either 0 to 1,600 mug/ml ammonium nitrate or 0 to 100 mug/ml urea. observations with scanning and transmission electron microscopy indicated that an elevated concentration of ammonium nitrate or urea inhibited giant cell formation and suppressed nematode development in the infected soybean roots. in the tomato roots, concentrations of amm ...199519277295
description of the kona coffee root-knot nematode, meloidogyne konaensis n. sp.meloidogyne konaensis n. sp. is described from coffee from kona on the island of hawaii. the perineal pattern of the female is variable in morphology, the medial lips of the female are divided into distinct lip pairs, and the excretory pore is 2-3 stylet lengths from the base of the stylet. mean stylet length is 16.0 mum, and the knobs gradually merge with the shaft. the knobs are indented anteriorly and rounded posteriorly and the dorsal esophageal gland orifice (dego) is long, 3.5-7 mum. the m ...199419279905
methods for the study of verticillium chlamydosporium in the rhizosphere.methods for screening isolates of the nematophagous fungus, v. chlamydosporium, for their ability to colonize the surface of plant roots are described. significant differences in the extent of colonization were observed in sterile conditions and in soil; plant species and cultivars also differed in their ability to support a selected isolate of the fungus. although fungal density could be estimated using a semi-selective medium, it was not possible to separate differences in vegetative growth fr ...199419279929
occurrence of pasteuria spp. in florida.two years of data collected from the florida nematode assay laboratory of the florida cooperative extension service and 4 years of data from the florida department of agriculture and consumer services, division of plant industry, were compiled to find out the distribution of pasteuria spp. on nematodes in florida soils. information recorded came from 335 samples and included nematode genera with pasteuria endospores attached, host plants associated with the samples, and the origins of the sample ...199419279936
response of meloidogyne spp. to pasteuria penetrans, fungi, and cultural practices in tobacco.the response of a mixed population of meloidogyne incognita and m. javanica to three cultural practices, tobacco cultivars (two cultivars, differing in resistance to m. incognita), cover treatments (three treatments), and inorganic nitrogen fertilizer (two treatments), pasteuria penetrans, and soil-borne fungi was investigated in a tobacco field in 1991. on all sampling dates, higher densities of root-knot nematodes were observed on tobacco cv. coker 371 gold than on k-326. initially, forage sor ...199419279937
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